LOCUS       KO-first_condition_ready_22832_MGI:1921054        20340 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Kif2c targeting project(s): 22832
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_gt1
COMMENT     design_id: 44460
COMMENT     backbone: L3L4_pZero_DTA_kan
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     promoter        85..598
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             612..1268
                     /note="DTA"
     misc_feature    1393..1651
                     /note="BGH pA"
     CDS             complement(2147..2941)
                     /note="KanR"
     misc_feature    4053..4073
                     /note="B3 Gateway"
     misc_feature    4079..4086
                     /note="AsiSI"
     misc_feature    1..4111
                     /note="Synthetic Backbone"
     exon            7720..7839
                     /db_xref="ENSEMBL:ENSMUSE00000181266"
                     /rank="6"
                     /note="ENSMUSE00000181266"
     exon            6433..6555
                     /db_xref="ENSEMBL:ENSMUSE00001235990"
                     /rank="5"
                     /note="ENSMUSE00001235990"
     misc_feature    4112..8580
                     /note="5 arm"
     misc_feature    8608..8632
                     /note="B1 Gateway"
     misc_feature    8645..8692
                     /note="Frt"
     intron          8693..9567
                     /note="mouse En2 intron"
     misc_feature    9565..9567
                     /note="Splice Acceptor"
     exon            9568..9606
                     /note="mouse En2 exon"
     misc_feature    9607..9623
                     /note="Frame 1 insert"
     misc_feature    9624..9677
                     /note="'self-cleaving' 2A peptide"
     gene            9678..12755
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    12756..12809
                     /note="'self-cleaving' 2A peptide"
     gene            12810..13601
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    13800..14042
                     /note="SV40 polyadenylation site"
     misc_feature    14059..14106
                     /note="Frt"
     misc_feature    14113..14146
                     /note="loxP"
     misc_feature    9582..9697
                     /note="FCHK std_gt1"
     misc_feature    9582..9599
                     /note="FCHK QC prefix region"
     misc_feature    9600..9679
                     /note="FCHK QC critical region"
     misc_feature    9680..9697
                     /note="FCHK QC suffix region"
     misc_feature    14162..14182
                     /note="B2 Gateway"
     misc_feature    8581..14212
                     /note="Synthetic Cassette"
     exon            15138..15233
                     /db_xref="ENSEMBL:ENSMUSE00000181263"
                     /type="targeted"
                     /rank="8"
                     /note="target exon 1 ENSMUSE00000181263"
     exon            14565..14665
                     /db_xref="ENSEMBL:ENSMUSE00000181253"
                     /type="targeted"
                     /rank="7"
                     /note="target exon 2 ENSMUSE00000181253"
     misc_feature    14213..15419
                     /note="Critical Region"
     misc_feature    15443..15476
                     /note="Downstream LoxP"
     misc_feature    15420..15499
                     /note="synthetic loxP region"
     exon            19956..20047
                     /db_xref="ENSEMBL:ENSMUSE00000510423"
                     /rank="13"
                     /note="ENSMUSE00000510423"
     exon            19816..19879
                     /db_xref="ENSEMBL:ENSMUSE00000181258"
                     /rank="12"
                     /note="ENSMUSE00000181258"
     exon            19544..19634
                     /db_xref="ENSEMBL:ENSMUSE00000181267"
                     /rank="11"
                     /note="ENSMUSE00000181267"
     exon            16931..17093
                     /db_xref="ENSEMBL:ENSMUSE00000181251"
                     /rank="10"
                     /note="ENSMUSE00000181251"
     exon            16789..16843
                     /db_xref="ENSEMBL:ENSMUSE00000181262"
                     /rank="9"
                     /note="ENSMUSE00000181262"
     misc_feature    15500..20340
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagataaacg
       61 gatcccctag tttgtgatag gatcgaattc taccgggtag gggaggcgct tttcccaagg
      121 cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc
      181 tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc
      241 cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc
      301 gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac
      361 agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc
      421 tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc
      481 gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt
      541 caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc
      601 aggtcctcgc catggatcct gatgatgttg ttgattcttc taaatctttt gtgatggaaa
      661 acttttcttc gtaccacggg actaaacctg gttatgtaga ttccattcaa aaaggtatac
      721 aaaagccaaa atctggtaca caaggaaatt atgacgatga ttggaaaggg ttttatagta
      781 ccgacaataa atacgacgct gcgggatact ctgtagataa tgaaaacccg ctctctggaa
      841 aagctggagg cgtggtcaaa gtgacgtatc caggactgac gaaggttctc gcactaaaag
      901 tggataatgc cgaaactatt aagaaagagt taggtttaag tctcactgaa ccgttgatgg
      961 agcaagtcgg aacggaagag tttatcaaaa ggttcggtga tggtgcttcg cgtgtagtgc
     1021 tcagccttcc cttcgctgag gggagttcta gcgttgaata tattaataac tgggaacagg
     1081 cgaaagcgtt aagcgtagaa cttgagatta attttgaaac ccgtggaaaa cgtggccaag
     1141 atgcgatgta tgagtatatg gctcaagcct gtgcaggaaa tcgtgtcagg cgatctcttt
     1201 gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa
     1261 agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt
     1321 gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgca
     1381 gatcctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     1441 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     1501 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     1561 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     1621 gtgggctcta tggcttctga ggcggaaaga accagctggg gctcgacgcg ccgtacccag
     1681 cttttgttcc ctttagtgag ggcctgaatg gcgaatggac gcgccctgta gcggcgcatt
     1741 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     1801 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     1861 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     1921 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     1981 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     2041 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     2101 ctattggtta aaaaatgagc tgatttaaca aaaattttaa caaaattcag aagaactcgt
     2161 caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga
     2221 ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta
     2281 tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc
     2341 cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcctcgc
     2401 cgtcgggcat gctcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct
     2461 cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga
     2521 tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc
     2581 gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat
     2641 cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga
     2701 gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtctt
     2761 gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg
     2821 ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc
     2881 cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca
     2941 tgcgaaacga tcctcatcct gtctcttgat cagatcttga tcccctgcgc catcagatcc
     3001 ttggcggcga gaaagccatc cagtttactt tgcagggctt cccaacctta ccagagggcg
     3061 ccccagctgg caattccggt tcgcttgctg tccataaaac cgcccagtct agctatcgcc
     3121 atgtaagccc actgcaagct acctgctttc tctttgcgct tgcgttttcc cttgtccaga
     3181 tagcccagta gctgacattc atccggggtc agcaccgttt ctgcggactg gctttctacg
     3241 tgaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     3301 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     3361 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     3421 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     3481 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     3541 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     3601 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     3661 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     3721 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     3781 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     3841 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     3901 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     3961 ttttacggtt cctgggcttt tgctggcctt ttgctcacat gtaatacgac tcactatagg
     4021 gcgaattgga gctaggcggc cgtttatcgt gccaacttta ttatacaaag ttgcggacgc
     4081 gatcgccggc ggataacaat ttcacacagg aacaataata atcataaact taaaggtgaa
     4141 gggctggcaa gataattcag gtaaaaggat tgtgtgccac agtcctgaca acctgagttt
     4201 aatcttaaat ggagaacgag aaaaggtaac agagtgtctt cggctctcca cacgtgctcc
     4261 acgtgtgcag cttcacacac acacacacac acacacacac acacacacac acacacacac
     4321 acacacacac acggggcctg caggaagagc tcgggtacca tcccagtgca agtctcacag
     4381 gatgtagatc ctttcctggt tggttggttc ttgttgatct tagcggcatg gtcccacaca
     4441 ggcagaagta ctactcactt ttggaagtca gtgccctcct tccaccttgg gctccgtggt
     4501 cagactcagg tcagcaggct gtgcagcaag cgccccctga tgagttgtgg gttttttttt
     4561 tttttattta ttttttaatg ttttagattc ttcttatttt ttttaaattc tgtatttttt
     4621 tagtatatgc tacatacaaa tgcaggtgcc caaagaggct agaggcatca gaatcttgta
     4681 gagctagagt tagaagccat tgtgagtcac tttagtctta actgctgaac catctcagct
     4741 ctggtatcca ggttttttga gaaagggtct cttgttcagc ctgtcctgga actcactgtg
     4801 taacccaggc tgacctctaa gttgtaggcc tactatctca gccttctcta agtgttagga
     4861 ttgtaggtgt tggcattcat tcagcctgac ttggaaaaaa agattatttt gtttgtttgg
     4921 catttcaaga cagggtttct ctgtgtagcc ctggctgtcc tggaacttat tctgtagacc
     4981 aggctggcct cgaactcaga gatcccccct gcctctgcct cccaggtact agaattaaat
     5041 gtttgtatca ctaacttgaa gggctttata gatgactaat tgtttctctg tcttactaca
     5101 gctacaagaa ttcaggtgtt ggggctggag agatggctca gtggttaaga gcattggctg
     5161 atctaccaga ggaattaggt tcaattccca tcacctatat ggcagctcac gactgtctat
     5221 aactgcagtt ccaagggatt tgacactctc acacagacat gtgccagcaa aacactaatg
     5281 cccataaaat aaataacata catacataca tacatacata cacacacacg cacacacaca
     5341 tatatatatg tatgtatgta tatatatatg tatgtatgta tatatatata tatatatatg
     5401 ttcagtaaca gcttacctat gtctcagtta taacctttcc ttgagatttc cgaatagttg
     5461 gtccatgggc ttctgaagag gtggcctgtc tcagtcctga taaagcaccc taggacagtg
     5521 gggaaagccc agattttgtc atgacagccc atcacaccct ggctttagcc attctggctt
     5581 cttctcattc tctggctcct gacttcacct gtgtctagct tgttctcttc aacctgtgtc
     5641 tttaaactcc ccagtcaaac tgtctcctcc tcctctccct ttctccccat tgctccctaa
     5701 gcagcttccc tttcccttct cttctagtgg cagatcctgg tctgttaagt ctttctctga
     5761 ttcatcactt tgtctgcccc tcaatcggaa gtcattttca aacatgcgtg cttccttcta
     5821 caaactaact ttactctcat tgtttgggat taaaggtgtg tactaagggt atgtctgcac
     5881 tccagccaga gggattaaag aacaagaaac agttttccag taaacaacac aatcttggca
     5941 ttcacaatgt gatcaagata tcctgcaaca gtcacgttaa ccctgagcca tctctccatc
     6001 cctaaaaata taaagtgaat cttttaaaaa gccccccccc ccccccgcat caagacttag
     6061 aagaataggt tttcctctcc agaaatgatt ttagatttgc tgtaggtcct tccatttatt
     6121 tattcatccc ctccttaccc tcctgactgc ctcccatact aggaattgaa cccagggcca
     6181 catacattct agattagcac ttgaccactg agctatattg ccagcccaag acaaatgttc
     6241 ttaagggaga taactttgcc cacaggagca caggtctacc aactgcacaa atagtttggg
     6301 gtagcagttc ttggtcttcc aaagtgcttg ctttcccgga ctcatttccg ggtggctagg
     6361 atctggcctg ccttgtgtgg aagctgctgc tttagctcat cttgtagcac tgattgatcc
     6421 cttcattctt aggccttcga agccgctcca ctcgcatgtc cactgtctca gaagttcgaa
     6481 tccctgctca ggagaatgaa atggaggtgg agctgcctgt gcctacaaac tctcgcaagc
     6541 agtttgccat tcccagtgag tgatgagtgt gttcttgtgt aagccctcag cagctccttt
     6601 tgcttcacat gagcccactg cattcccagg gtcccggcat gcgtacttca ggatgggctt
     6661 tgtcctgaag ggaaaagcct ccgaggctgc ctgactctac agacttgagc caagcatggc
     6721 ttctcaggcc aagccagccc tttctgactt ttccaaagcc ccagaaacca cgtggttagc
     6781 agaagtggcg aaggtggggg agaagaaaac aacatggatg acgatgactg ctgcttgggc
     6841 aacagttaac tctatgggct cagtgaacca gtgactgtgg ttgtcgcaat taagatgcca
     6901 gtacttaaga gattgccacg ggtttgagat cagtctgggc tacagggtga aactctgtct
     6961 ccatatatac acatgagcac tcatgccgtg cacaggcaca cacacccacc aagaacaaca
     7021 aaaattcaaa ctggaaaaaa aaaaaacagc agaaaggatg atctatcacc ccttcctgcc
     7081 ctcatgtgtg gtcctgatcc tttcatgtag atggatttaa tacatacaac ataatctagt
     7141 gcctcgtaaa atcttcatct actttaccca accagtggag agagcccttt aattaaaatt
     7201 attattgggt tggggttata gctcaattgt tagactgctt gcctgggttc cattcctggt
     7261 ggcacataag atggcatagt ggacctcatc ggagagtcta gcgctcacgc ggcaggagga
     7321 ttacaagttt gacttaacct cagttcaatc atagtgagtt ggaggtcagc ctaggctatg
     7381 tgattccctg tttcaaaaca attgttttgg gacagggtct ctctttcctt ctttaccctg
     7441 tgatagccta gaatttaccc taggctgccc ttggacttgg aaaaaaatca tcctccctta
     7501 gccttccgag tgctgggatt acaggtggga gctgctttga tgtttagttt atggttcttg
     7561 agccaggtgt ggtccctggc tggcaggtgg ttggattcgg gatgagggca gaggaaaagc
     7621 ctaaatacgg cccttggccc cccaccccca cccccgagat tcaaggacaa ctgctccatg
     7681 ctgcccgctt ctccagtgac tcttgttctt ccactgcagg ccaccctcgg gcctcctgct
     7741 ccacagtgac agaattgccg ttgttgatgg tcagtgagga ggcagaagag caagcccact
     7801 ccacccgcag tacctcatct gcaaaccctg ggaactcagg tagataggct gggccctgtg
     7861 tgcttgccct ctagcacagg acaggagtta acactgggcc tgcctgggtc caagttcaaa
     7921 cccttttgtt accttgaagg tatgttaccc agtgcttatg gactgagtct tccacaagac
     7981 acagtttgtt agaagccatt tggtgagata gatggactta caacccagag gactttccct
     8041 agtgcagtgg gaatggcttc tttttttttt tttttttaaa ttaagattta ttgattttat
     8101 gattttagcc agatgtagtg gctcatgcct ttgatcccac catttgggga gcagaggcag
     8161 gtgaattgtg ggttcgaggc cagcctggtc tatagaggga gtttcaggac agccacagct
     8221 acacagagaa atcttgtctg gaaaaacaaa caaaaagatt tattgatttt tattgtattt
     8281 tttttaatgt gtatgagtgt tttaattgta tgcatgtctg tgtacctggt gcctgtggaa
     8341 gtcagaagag ggcatcagat ctcctcggtt gtgagctacc atgtagatgc tgggaatcaa
     8401 tcctgggtct tctgcaagag caaatggtct tagtcaccga gccacctctc cagccctgcc
     8461 tcattttgat tctacaggaa ctagctgcat aagcataaag agagcttagg tcaaaaccag
     8521 agtgggatga ttatcactct gacgtcactc tagcccagtt agtgctccat ggactcgtcc
     8581 aaggcgcata acgataccac gatatcaaca agtttgtaca aaaaagcagg ctggcgccgg
     8641 aaccgaagtt cctattccga agttcctatt ctctagaaag tataggaact tcgaaccctt
     8701 tcccacacca ccctccacac ttgccccaaa cactgccaac tatgtaggag gaaggggttg
     8761 ggactaacag aagaacccgt tgtggggaag ctgttgggag ggtcacttta tgttcttgcc
     8821 caaggtcagt tgggtggcct gcttctgatg aggtggtccc aaggtctggg gtagaaggtg
     8881 agagggacag gccaccaagg tcagcccccc ccccctatcc cataggagcc aggtccctct
     8941 cctggacagg aagactgaag gggagatgcc agagactcag tgaagcctgg ggtaccctat
     9001 tggagtcctt caaggaaaca aacttggcct caccaggcct cagccttggc tcctcctggg
     9061 aactctactg cccttgggat ccccttgtag ttgtgggtta cataggaagg gggacgggat
     9121 tccccttgac tggctagcct actcttttct tcagtcttct ccatctcctc tcacctgtct
     9181 ctcgaccctt tccctaggat agacttggaa aaagataagg ggagaaaaca aatgcaaacg
     9241 aggccagaaa gattttggct gggcattcct tccgctagct tttattggga tcccctagtt
     9301 tgtgataggc cttttagcta catctgccaa tccatctcat tttcacacac acacacacca
     9361 ctttccttct ggtcagtggg cacatgtcca gcctcaagtt tatatcacca cccccaatgc
     9421 ccaacacttg tatggccttg ggcgggtcat cccccccccc acccccagta tctgcaacct
     9481 caagctagct tgggtgcgtt ggttgtggat aagtagctag actccagcaa ccagtaacct
     9541 ctgccctttc tcctccatga caaccaggtc ccaggtcccg aaaaccaaag aagaagaacg
     9601 cagatcgcag atcgcagatc tccgagggca gaggaagtct tctaacatgc ggtgacgtgg
     9661 aggagaatcc cggccctggg atctggactc tagaggatcc cgtcgtttta caacgtcgtg
     9721 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     9781 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     9841 atggcgaatg gcgctttgcc tggtttccgg caccagaagc ggtgccggaa agctggctgg
     9901 agtgcgatct tcctgaggcc gatactgtcg tcgtcccctc aaactggcag atgcacggtt
     9961 acgatgcgcc catctacacc aacgtgacct atcccattac ggtcaatccg ccgtttgttc
    10021 ccacggagaa tccgacgggt tgttactcgc tcacatttaa tgttgatgaa agctggctac
    10081 aggaaggcca gacgcgaatt atttttgatg gcgttaactc ggcgtttcat ctgtggtgca
    10141 acgggcgctg ggtcggttac ggccaggaca gtcgtttgcc gtctgaattt gacctgagcg
    10201 catttttacg cgccggagaa aaccgcctcg cggtgatggt gctgcgctgg agtgacggca
    10261 gttatctgga agatcaggat atgtggcgga tgagcggcat tttccgtgac gtctcgttgc
    10321 tgcataaacc gactacacaa atcagcgatt tccatgttgc cactcgcttt aatgatgatt
    10381 tcagccgcgc tgtactggag gctgaagttc agatgtgcgg cgagttgcgt gactacctac
    10441 gggtaacagt ttctttatgg cagggtgaaa cgcaggtcgc cagcggcacc gcgcctttcg
    10501 gcggtgaaat tatcgatgag cgtggtggtt atgccgatcg cgtcacacta cgtctgaacg
    10561 tcgaaaaccc gaaactgtgg agcgccgaaa tcccgaatct ctatcgtgcg gtggttgaac
    10621 tgcacaccgc cgacggcacg ctgattgaag cagaagcctg cgatgtcggt ttccgcgagg
    10681 tgcggattga aaatggtctg ctgctgctga acggcaagcc gttgctgatt cgaggcgtta
    10741 accgtcacga gcatcatcct ctgcatggtc aggtcatgga tgagcagacg atggtgcagg
    10801 atatcctgct gatgaagcag aacaacttta acgccgtgcg ctgttcgcat tatccgaacc
    10861 atccgctgtg gtacacgctg tgcgaccgct acggcctgta tgtggtggat gaagccaata
    10921 ttgaaaccca cggcatggtg ccaatgaatc gtctgaccga tgatccgcgc tggctaccgg
    10981 cgatgagcga acgcgtaacg cgaatggtgc agcgcgatcg taatcacccg agtgtgatca
    11041 tctggtcgct ggggaatgaa tcaggccacg gcgctaatca cgacgcgctg tatcgctgga
    11101 tcaaatctgt cgatccttcc cgcccggtgc agtatgaagg cggcggagcc gacaccacgg
    11161 ccaccgatat tatttgcccg atgtacgcgc gcgtggatga agaccagccc ttcccggctg
    11221 tgccgaaatg gtccatcaaa aaatggcttt cgctacctgg agagacgcgc ccgctgatcc
    11281 tttgcgaata cgcccacgcg atgggtaaca gtcttggcgg tttcgctaaa tactggcagg
    11341 cgtttcgtca gtatccccgt ttacagggcg gcttcgtctg ggactgggtg gatcagtcgc
    11401 tgattaaata tgatgaaaac ggcaacccgt ggtcggctta cggcggtgat tttggcgata
    11461 cgccgaacga tcgccagttc tgtatgaacg gtctggtctt tgccgaccgc acgccgcatc
    11521 cagcgctgac ggaagcaaaa caccagcagc agtttttcca gttccgttta tccgggcaaa
    11581 ccatcgaagt gaccagcgaa tacctgttcc gtcatagcga taacgagctc ctgcactgga
    11641 tggtggcgct ggatggtaag ccgctggcaa gcggtgaagt gcctctggat gtcgctccac
    11701 aaggtaaaca gttgattgaa ctgcctgaac taccgcagcc ggagagcgcc gggcaactct
    11761 ggctcacagt acgcgtagtg caaccgaacg cgaccgcatg gtcagaagcc gggcacatca
    11821 gcgcctggca gcagtggcgt ctggcggaaa acctcagtgt gacgctcccc gccgcgtccc
    11881 acgccatccc gcatctgacc accagcgaaa tggatttttg catcgagctg ggtaataagc
    11941 gttggcaatt taaccgccag tcaggctttc tttcacagat gtggattggc gataaaaaac
    12001 aactgctgac gccgctgcgc gatcagttca cccgtgcacc gctggataac gacattggcg
    12061 taagtgaagc gacccgcatt gaccctaacg cctgggtcga acgctggaag gcggcgggcc
    12121 attaccaggc cgaagcagcg ttgttgcagt gcacggcaga tacacttgct gatgcggtgc
    12181 tgattacgac cgctcacgcg tggcagcatc aggggaaaac cttatttatc agccggaaaa
    12241 cctaccggat tgatggtagt ggtcaaatgg cgattaccgt tgatgttgaa gtggcgagcg
    12301 atacaccgca tccggcgcgg attggcctga actgccagct ggcgcaggta gcagagcggg
    12361 taaactggct cggattaggg ccgcaagaaa actatcccga ccgccttact gccgcctgtt
    12421 ttgaccgctg ggatctgcca ttgtcagaca tgtatacccc gtacgtcttc ccgagcgaaa
    12481 acggtctgcg ctgcgggacg cgcgaattga attatggccc acaccagtgg cgcggcgact
    12541 tccagttcaa catcagccgc tacagtcaac agcaactgat ggaaaccagc catcgccatc
    12601 tgctgcacgc ggaagaaggc acatggctga atatcgacgg tttccatatg gggattggtg
    12661 gcgacgactc ctggagcccg tcagtatcgg cggaattcca gctgagcgcc ggtcgctacc
    12721 attaccagtt ggtctggtgt caggggatcc cccgggaggg cagaggaagt cttctaacat
    12781 gcggtgacgt ggaggagaat cccggcccta ttgaacaaga tggattgcac gcaggttctc
    12841 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
    12901 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
    12961 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca
    13021 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
    13081 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
    13141 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
    13201 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
    13261 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
    13321 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
    13381 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
    13441 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
    13501 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
    13561 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga
    13621 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt
    13681 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
    13741 cggggatctc atgctggagt tcttcgccca ccccccggat ctaagctcta gataagtaat
    13801 gatcataatc agccatatca catctgtaga ggttttactt gctttaaaaa acctcccaca
    13861 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
    13921 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
    13981 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
    14041 ccgggggtac cgcgtcgaga agttcctatt ccgaagttcc tattctctag aaagtatagg
    14101 aacttcgtcg agataacttc gtatagcata cattatacga agttatgtcg agatatctag
    14161 acccagcttt cttgtacaaa gtggttgata tctctatagt cgcagtaggc ggtaggaggg
    14221 gagaactgac ttctaaaaat taaacctgac ttccacatgt atggtgtgca tgagcgtgcg
    14281 tacaaatata cactctaatt tacaaataag ccggacatgg tggctcacac ctttgacccc
    14341 agaatgtggg aggcaaagga gggcaaatct gatctataaa ggagatcagc ctgatgtatc
    14401 tacagtgagt tctgagatag ccagagctac atggaggaaa aagccaaaat aagcagaagc
    14461 agagagggtc tagagaaaga tgacctgttt tatgaaagat ccagtgctca gggaatagcc
    14521 agagactgac agagggaacc cggtgtgttt ccttctcttc ttagttcgga ggaaatcgtg
    14581 tatcgtgaag gagatggaga aaatgaagaa caagcgtgaa gagaagaggg cccagaactc
    14641 ggagctaaga ataaagcgag cacaggtgac tgtcgggaga tgtggtcgag agtttccgca
    14701 caggtcccta acagatgtgc agtcagctga tgaccagctg agccatactt ggtgctgatt
    14761 ggccagtggg gtagcccatc ttgtcctcac aaggtgtaat gcttttgctg ggcacaccta
    14821 cttgctttct tgtccatccc ctgtaagctg tattatcaca gggtgaaatc ggtggcaaag
    14881 aaggctcctt agctgggtaa ctgctctgag gaaacccatt ctgttgcttt gcctcctaag
    14941 atggtgaaag cagctctcgt ccttggtgca tgcctatgct cccccacagt cctgcctcag
    15001 aactttgtgc ctggcttctc agactggagc cggggtgagg cactccttag ctgcggagct
    15061 gttgctgcct ggtagctctg ggtctaagag cacccgcacc tgaacttttg tccttctgtc
    15121 cctcctgcct cacacaggaa tatgacagca gctttccaaa ctgggaattt gcccggatga
    15181 tcaaagaatt tcgggttact atggaatgta gcccgcttac tgtgacagac cctgtgagta
    15241 taccagaaag tctcctcctc ttccctgagc aggtaaacca ctgaaggaca aagagaaagc
    15301 atgcagggat caccatgcag tcttgagtgt tatctgaggg agtgcttctc tctctctctc
    15361 tctctctctt tttctatgtg tgtgtgtgtt aatggggatt gaatttggta ctccatatag
    15421 agatggcgca acgcaattaa tgataacttc gtatagcata cattatacga agttatggtc
    15481 tgagctcgcc atcagttcaa cttgttctcc caaaacaccg gggttcgatt ccctacaggt
    15541 gtctcacaac cagctgtaaa tcccctgctt cctgggcact gctcccatgt gatacacaga
    15601 catgtacagg ccggcactct tacatattta caaaaacgaa agggtcacac tttagaactg
    15661 cctggcctag aacttgctgt gtagactaaa cttgccttaa actcacacaa atctgcttgc
    15721 ctgtctccca ggtgctagaa ttacagtgac atggcagaac acccagtaaa caatgtcctt
    15781 aaattagctt tgtttcccct ggcagggtgc ttgtgctcac tcccatatgg atgacaggac
    15841 agcttgcagg actcagttct ctaacgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg
    15901 tgtgtgtgtg tatgtgtatg tgcgggtgcg cgtgtgcacg tgccagggat tgaacttggg
    15961 tcttcaggct tggtgtcctg actgcctgta tctgctgagc catctcccag gctggttttt
    16021 gtttgtttgg ctggattttt ttccccttct tgagacagag tctcaatgta tggtgagatt
    16081 acaggtatat gctgtcaaac tttgccttaa aaaaacaaag caaggcctag agacatgact
    16141 cagcagttaa gagcacaggc tgctcttcca gaggtcaagt cccactaacc tcatggtggc
    16201 tcgcaactat ctataatggg atctgatgcc ctcttctggt gtgtctgaag atagcaacaa
    16261 tgtattcata caatagaata caataaacaa ataattctct aaaaaaataa aaaccaaaaa
    16321 ccaaacaaaa ttctgggggt attgcacctg tgtggccatg ccgtggagaa ggtcaggaac
    16381 agcttactga gttgggcctc ctgtcttgtg ggttccaggt atggaactga ggtcatccgg
    16441 cttggcaaag gagctcttgc ccacccggcc atctcagcag ctagattttt tgtttgtttt
    16501 taaatacttt aggggagaac attagttaag gctcagatga ggcactcgct ctctgcttta
    16561 ggctggcctg actgctaagg gctagttgag tagtgctgaa tccttgaagg ccatggagta
    16621 agagaacgag ggccattcag aatgactttc ttgtccccta gagtggttaa cagggttcta
    16681 gtatcaggtt tctttgtccc ttgctagagc caagaaaggg gttgtggata aactctgctt
    16741 tggtactgtt ctgacctccc gttggctgtc tccctcattc tcttgcagat cgaagagcac
    16801 aggatctgtg tctgtgtcag gaaacgccca ctgaataaac aaggtgagct gttgagtcag
    16861 aagcagaggg ctgactgaga ggaaggtcct gtgttcagag agcagcccct gaaatcttct
    16921 ccttctgcag aactggccaa gaaagaaatc gatgtgattt ctgttcccag caagtgtctc
    16981 ctcctggtgc atgaacccaa gttaaaagtg gacttaacga agtacctgga gaaccaagct
    17041 ttctgctttg actttgcatt tgatgagaca gcttccaatg aagttgtcta caggtgagcc
    17101 ctgtgctctc gcttatcctc ctcatgccac tttctccccc aagtctctct ctgtctgaca
    17161 gcggacgtcg aactcctccc tgatctgtcg agttttactg ggttagctct ctgattcagg
    17221 agcactggcc cctctaactg tgaggtcttg tttgggacct gccatgtcac cttttaagct
    17281 atagcagccc agccttagat gactcatgag tgagacatta ccactgcact gaggatcatg
    17341 ggtaggcacc agcagttctc ttcgatgttt tgttgggaca agagctcatc tgtagcccag
    17401 gctatcctag acctgtctgt gctgctcgct cacatggtct tgaaccatag cagtcctgcc
    17461 tcagtctccc aagtgctgag attacacttt ccaatggttt atgcagctac ctcatgaccc
    17521 acatgagctt agcagcctca ggtatcttca gatctactcg gtgcagtgtg ccactcagca
    17581 cctaagctta catctttggt tgtgagctta gtctttcatg tctgagccat ctctccgtcc
    17641 cagcatttaa ttcacagtga aggtttcagg gagggggttg gtggtggggt gtgtttgtgt
    17701 ggatgcatga ggagaaaatg gacatggttg tgccatggtg tgtgtgtaga agtcaggaca
    17761 gctttgagag gtcagctctc cttccacgag tgcagactta gacagaaagc actttttatc
    17821 agctaagccc cagccttcaa ggctttgagt ttgtcagaca ttttttttta aaagaggttt
    17881 ggaggtgcag tcttacactt ctggtgctgg ggaagctgag gtaataggtc ttgacattgg
    17941 ccaggcagtg gtgacacaca cctttaatcc cagcactcgg gaggcagagg caggcagatt
    18001 tctgagttcg aggccagcct ggtctacaga gtgagttcca ggacagccag ggctatacag
    18061 agaaaccctg tctcgaaaaa acaaaacaaa acaaaaaacg gacacaggag ggtttggaac
    18121 ttaaggccag cctaggctat agtgtgagat cctaattatg acagacattc tatgtagttc
    18181 acatacgaca tattttcggc ctggttttct tttgcccttt gtaatacatc ctgagcagtg
    18241 gggtcagagg aggggctgtt tggcttaaag tctggatcat cagcttgttc ctggtaggtg
    18301 agttggacac tgcctgcacc cttttattta gctagtagta cagattggtt agtgggatgg
    18361 atcagggtag accttgagag gaatgctcta cttaaggaat taagacttgc agcttgaccc
    18421 gggtgtggtg gcgcacgcct ttaatccagc actcgggagg cagaggcagg caaatttctg
    18481 agtttgaggc cagcctggtc tacagagtga gttccaggac agccagggct atacagagaa
    18541 accctttctc aaaaaaaaaa aaaaaaaaaa aaagacttgc agcttgacag gcagtggtgg
    18601 tcacaccttt aattccttta atttcttgag ttggaagcca gcctggtcta cagagagttc
    18661 caggacagcc agggctgtac agagaaaccc tgtcttttgg tttttgtttg tttggttttt
    18721 gtttttccag acagtttctc cgtacagtcc tggctgtcct agaactcact ctgtagatca
    18781 gactggtctc aaactcagat ctgcctgcct ctgcctccga agtgctggga ttaaaggtgt
    18841 gcaccaccgc tgcccagctg aaaccctgtc ttaaaaaaat gaaccaaaca aaaagacttg
    18901 gagctctaca aaggaccatg ttttagagag cacattttgc agtttttcct tcatgttctc
    18961 gagttgctgt ggactcaacc acaaagggaa aatacttact tccgaaagag cggcctgtga
    19021 gacggcttac caagcctggc gacctaagcc ggggccccag gttagggtgg caggaggaga
    19081 aaactgactt ccacacgtgg tctgctgacc ttgccatgtg tcacaccagg tcaccaaccc
    19141 atgtgcacac agagaaatac atggaattga aaagttagaa agaaccgttt gtcctaaaca
    19201 tagttctttt catttttccc cctgaacagt agagtgtaag gaatatatca tctttctatc
    19261 ttattaggat atagataatc tagagatatg taatagtgta tgtaagtact gtgcaaacat
    19321 gatgccagtt tagataatgg acctgagtaa gagtcaactc agtgacagtg tttgctcagc
    19381 atgctaggcc ctgggttctg cccagaagaa agaaagaaaa gaaagggaag tgatttacca
    19441 gcagccatgt gctgagtgtc cgggggttcc cgtagccagt acctcttgga ctctaaggga
    19501 tagctacaca gatgcacttc ctccacactg tcttttctcc taggttcaca gcaaggccac
    19561 tggtacagac gatttttgaa gggggaaaag caacatgctt tgcctatgga cagacaggca
    19621 gtgggaagac acatgtgagt atttcagtca ggagagaaag ctttactaag ttcttgagaa
    19681 ggaagacaga tgcccagtca actagcattt gccatgccag atgcagagag gggctttaga
    19741 taggtccatc ctcctgactc tggcatgggc cactgtgttc tactgggctg tgtcacatgc
    19801 ggtttccctt tgcagacaat gggtggagat ctgtctggta aaagtcagaa cgcatctaaa
    19861 gggatctacg ccatggcttg tgagtaaact gtttggctac attggggttg ggtatggaca
    19921 gcaagctgct tactgaggga ggctcttcct tgcagcccgg gatgtcttcc ttctgaagaa
    19981 ccagcctcgg taccggaact taaacctgga agtttatgtg acattcttcg agatctacaa
    20041 tgggaaggta aagtgactta aaaccttgtg tgccagccgc ccttttgttt gtttgtttct
    20101 ctgtgtagcc ctggctgtcc tggaactcac tctgtagacc aggctggcct cgaactcagc
    20161 aatccacctg tctctgcctc ctgaatgctg cgattaaagg catgcagcac caccacgtgg
    20221 tcggctgctg ttgtagttag ctttttgaat ttgtttgttt gcttttagta gtctcagctt
    20281 tatagacgga acaggctaga gtggttcagt aacttgctca gattggtaca gctagtaaaa
//