LOCUS       KO-first_condition_ready_31068_MGI:1917542        19881 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Afap1 targeting project(s): 31068
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_gt0
COMMENT     design_id: 43194
COMMENT     backbone: L3L4_pZero_DTA_kan
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     promoter        85..598
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             612..1268
                     /note="DTA"
     misc_feature    1393..1651
                     /note="BGH pA"
     CDS             complement(2147..2941)
                     /note="KanR"
     misc_feature    4053..4073
                     /note="B3 Gateway"
     misc_feature    4079..4086
                     /note="AsiSI"
     misc_feature    1..4111
                     /note="Synthetic Backbone"
     misc_feature    4112..8540
                     /note="5 arm"
     misc_feature    8568..8592
                     /note="B1 Gateway"
     misc_feature    8605..8652
                     /note="Frt"
     intron          8653..9527
                     /note="mouse En2 intron"
     misc_feature    9525..9527
                     /note="Splice Acceptor"
     exon            9528..9567
                     /note="mouse En2 exon"
     misc_feature    9562..9569
                     /note="Frame 0 insert"
     misc_feature    9570..9623
                     /note="'self-cleaving' 2A peptide"
     gene            9624..12701
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    12702..12755
                     /note="'self-cleaving' 2A peptide"
     gene            12756..13547
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    13746..13988
                     /note="SV40 polyadenylation site"
     misc_feature    14005..14052
                     /note="Frt"
     misc_feature    14059..14092
                     /note="loxP"
     misc_feature    9542..9643
                     /note="FCHK std_gt0"
     misc_feature    9542..9559
                     /note="FCHK QC prefix region"
     misc_feature    9560..9625
                     /note="FCHK QC critical region"
     misc_feature    9626..9643
                     /note="FCHK QC suffix region"
     misc_feature    14108..14128
                     /note="B2 Gateway"
     misc_feature    8541..14158
                     /note="Synthetic Cassette"
     exon            14509..14617
                     /db_xref="ENSEMBL:ENSMUSE00000185452"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 1 ENSMUSE00000185452"
     misc_feature    14159..14773
                     /note="Critical Region"
     misc_feature    14797..14830
                     /note="Downstream LoxP"
     misc_feature    14774..14853
                     /note="synthetic loxP region"
     exon            17334..17545
                     /db_xref="ENSEMBL:ENSMUSE00000185456"
                     /rank="5"
                     /note="ENSMUSE00000185456"
     misc_feature    14854..19881
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagataaacg
       61 gatcccctag tttgtgatag gatcgaattc taccgggtag gggaggcgct tttcccaagg
      121 cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc
      181 tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc
      241 cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc
      301 gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac
      361 agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc
      421 tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc
      481 gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt
      541 caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc
      601 aggtcctcgc catggatcct gatgatgttg ttgattcttc taaatctttt gtgatggaaa
      661 acttttcttc gtaccacggg actaaacctg gttatgtaga ttccattcaa aaaggtatac
      721 aaaagccaaa atctggtaca caaggaaatt atgacgatga ttggaaaggg ttttatagta
      781 ccgacaataa atacgacgct gcgggatact ctgtagataa tgaaaacccg ctctctggaa
      841 aagctggagg cgtggtcaaa gtgacgtatc caggactgac gaaggttctc gcactaaaag
      901 tggataatgc cgaaactatt aagaaagagt taggtttaag tctcactgaa ccgttgatgg
      961 agcaagtcgg aacggaagag tttatcaaaa ggttcggtga tggtgcttcg cgtgtagtgc
     1021 tcagccttcc cttcgctgag gggagttcta gcgttgaata tattaataac tgggaacagg
     1081 cgaaagcgtt aagcgtagaa cttgagatta attttgaaac ccgtggaaaa cgtggccaag
     1141 atgcgatgta tgagtatatg gctcaagcct gtgcaggaaa tcgtgtcagg cgatctcttt
     1201 gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa
     1261 agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt
     1321 gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgca
     1381 gatcctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     1441 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     1501 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     1561 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     1621 gtgggctcta tggcttctga ggcggaaaga accagctggg gctcgacgcg ccgtacccag
     1681 cttttgttcc ctttagtgag ggcctgaatg gcgaatggac gcgccctgta gcggcgcatt
     1741 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     1801 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     1861 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     1921 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     1981 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     2041 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     2101 ctattggtta aaaaatgagc tgatttaaca aaaattttaa caaaattcag aagaactcgt
     2161 caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga
     2221 ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta
     2281 tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc
     2341 cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcctcgc
     2401 cgtcgggcat gctcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct
     2461 cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga
     2521 tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc
     2581 gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat
     2641 cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga
     2701 gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtctt
     2761 gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg
     2821 ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc
     2881 cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca
     2941 tgcgaaacga tcctcatcct gtctcttgat cagatcttga tcccctgcgc catcagatcc
     3001 ttggcggcga gaaagccatc cagtttactt tgcagggctt cccaacctta ccagagggcg
     3061 ccccagctgg caattccggt tcgcttgctg tccataaaac cgcccagtct agctatcgcc
     3121 atgtaagccc actgcaagct acctgctttc tctttgcgct tgcgttttcc cttgtccaga
     3181 tagcccagta gctgacattc atccggggtc agcaccgttt ctgcggactg gctttctacg
     3241 tgaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     3301 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     3361 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     3421 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     3481 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     3541 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     3601 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     3661 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     3721 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     3781 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     3841 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     3901 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     3961 ttttacggtt cctgggcttt tgctggcctt ttgctcacat gtaatacgac tcactatagg
     4021 gcgaattgga gctaggcggc cgtttatcgt gccaacttta ttatacaaag ttgcggacgc
     4081 gatcgccggc ggataacaat ttcacacagg atttggatga gaatgttccc ccaagctggt
     4141 taatgttttc ctgacgtaaa gagacaccac caccatgaca accccactcc ttcctggcaa
     4201 gtcttaactt accagtctct ggcttacagt tcagaggttt agaccatttt tgtcctaata
     4261 ggacgcatgg tgccacacag gcagacgtgg tgctggagag gtagctaaga gaaaccctgt
     4321 ctcgaaaaac aaacaaacaa acaaacaaaa aaccagcaaa aaaaaaaaga gttctaaata
     4381 tggattggca gaagagagtg agagccattg ggtctgcctt gagtcacacc tactcaatga
     4441 ccaagcattc taatctatgg acttataggg ccattcctat tcaaaccacc acagctctga
     4501 tatttgaaga caatgtctcc agttgggggt gctgtttggg gaggcttggt tggggacatc
     4561 cttgctaaag gaaatgtgtc actgagtgtg ggctttgaga gcttataacc tgactcacag
     4621 agatccacct tcccctgcct gaggttgtca ccacaccctg ctttctcagc cattcttaag
     4681 tgtgcagttg gttgctgaat ccccgtggca cccttatttt gtctgacaga tgggattaga
     4741 gaacgcgttg ttatgatgtg ttagagttag gccttggcag tcagagtcag gcctggagct
     4801 actgattctg tgtgtggaca catagattct ctgtgtgacc ttaccatccc ctacctctct
     4861 tgtctttcca gcgtctcagc atgccatctc catgtggtct ggtggcatct ttcctttgca
     4921 ttcaacctgt ggaattttag gcatgcacct ggatcccatg ccttcagtct cctctgcttt
     4981 tctactcgga tgctacctcc taatggctct gcttctgcac tgtcataggt taaggctgag
     5041 aaagcataag aaaatgcgtg ctgggtcttg ctgggtagtc actgttgcat gcagatcaga
     5101 gagtgggcgg actggtgaac tctgtctaag gactgtctgt caagactaga attcttggtg
     5161 ttaggatgca atctatctat ctatctatct atctatctat ctatctatct atctatctat
     5221 ctactgatct accaatctat caatctattg atctatcttc agttttatag cccatgggag
     5281 aaaattcctg gactctaatc cccttggccg gggaaactca gactttgaca tgtttctctg
     5341 tgctgccaag catccctgct gctggtgtgg ggcagatctc cccagaactc tgattttctt
     5401 atttcagatt ggataattgt tgtctgttat tctataaata cattgatttt tttttttgag
     5461 ccaggaaagc aatatggatg catccctgga tgtgtagaat tggagtcaga acgtggtttc
     5521 tcagctatag tcagtttggg ggcttctcca gtgtttctta tgcaaagact tctgtgatgt
     5581 gtgaggtcat gtcatagaca gggggatggt gggggaggga gagctccagc tctgagaagt
     5641 gtttgggtgt aatggtgttg tgtaagatgg gggtggggag gtatgcctct ccttaggaag
     5701 caggaggtct ggatgtggtg atatggactc tgtagccccc tccctactcc cacagccctt
     5761 ctctgcttaa catcacaatg atgataccga ggggctcatc cacctgtaac tctagctcag
     5821 ggtccgatgt cctgttctct ctgcaggcac tgacatttat aacacataca ggtgtgcatg
     5881 tgtgcacata tgcgcatgcg ctcacactat caaaaaacaa tttttttttt taaaagtggt
     5941 agtattaaaa atgtaagcta tgcagatata ggtaggaaag gcaactttgt gctaacactt
     6001 ttggtaagca gtcagcttta gcagagacac tcaagaaatc tagacagagg ttggggccgc
     6061 agtgttcttt cacttagtgg tgctgactag ggttttttta aagtcagcag ctccaggctg
     6121 tgctgctgtg tgtggcagca catctgagca ttcttgtttt ttttaaacat taatttcttc
     6181 cttttttttt gttttttttt tgagacaggg tttctctgta tagccctggc tgtcctggaa
     6241 ctcactttgt agacccagct ggccttgaac tcagaaatcc gcctacatct gcctcccgag
     6301 tgctagcccg gctaaaacat taatttctat ttattattat ttttgtgtgt gagtgtggca
     6361 gaggtaccac agcatgcatg ggggaagtca gaggacaagg tctctcctct ggggatccgg
     6421 ggatcacact caggcagtcg gacttgcaca gtggcctggt tagggttttg ttgttgttgt
     6481 taacttgaag atcagcttgt aggcaggcct ccttgactga tgatggaagt ggatgatctc
     6541 agcctacacc gtacaccggt gccactcctg ggtaggagga cctgagaagg ataaagcagg
     6601 ctgagcaagc ctgcaagcca ccttcctccg gggcctttgc aggggggggg ggggttgtgt
     6661 tagctgctgc ccccaggctc ccgccccgcc ttttcttcct ggtgaactgt aggctgtgga
     6721 tagaatcagc aacctctcag agttgctttt ggccatggtg attatcgcac agcagaaagc
     6781 agaccaagat gcctcccagg cccttctacc tgctgggcca gcttgctggc cagttcttct
     6841 gacgcttgtt tatattttcc cagaaacttt tgctgagttt cttgagtttt tttttttttt
     6901 tctcctcatc tgcccaaagg accttcctgt tgtgcttgta gagatgaaac cctgaggtct
     6961 gcaggctatg tccttgctgg cttgactgtg tctgttcaca aacccctcag tacacactag
     7021 ccttttcccc tcttccctat tcgcagctcc ccggagggag gcctgacctg gatacagaca
     7081 gagacttagc tccagtccaa ggcgtgagca gtcagttttc cggcactcag aggaaggggg
     7141 aatccctgct ttctcatata ccttcaagca ctcagtgaat caaatggctc cccctgaagc
     7201 agcctccgat tccaggctgg atgtttagtg tcacccggtg ccaagcacag catatcaggt
     7261 ctcttcttag gacaggagag ccttggctga gccagcatgg gccctggggc agctttagag
     7321 gacatcccct ccagtgagtg ctggctgcct acaaagctgg tcctttatca tgtaggagga
     7381 tggcgaccac caggggacta ggtacatgca taggagggaa tggaagctgg tttctgcagg
     7441 gaacaggcct gggcagttgg gtaatagcat tccgctagct gaactgggat tcaagcctct
     7501 ccccagctgc aggaggagga agcagagtgg gtgtgcaatg gcccttagtc actcacaaag
     7561 gacttcttgg actcagagtt agggaaaatg acggagtcac attctggagg atttgcagaa
     7621 ggataaacac aaaggccatg atgtcagtgg agcagtgttc gggcaggatg gagcttgtca
     7681 cttctaccct gtcacttcct ggttatttac agaatctaga tagtgtgaat tcgctatgca
     7741 cagaggggga gagatcatta aagaaggtct ttaatagatg ccgggatgtc tggaaaccca
     7801 accatggcag gaacaagtct gattttgagc agcgtgcttt agattctggg ccttgctctc
     7861 tgcgcctgta tggtatgagg ctatccatgc tagccttccc gcagggctcc catgatacat
     7921 tgccaggcaa ggagcagggg ccctggtgca tcagacctca ttcccttcat ccttttctag
     7981 tggcagagga ccagcaagga caatcaccgg cagggtccca cagatgtcag agaagaggct
     8041 ggcatggact agttgtcagg aaagttcagt aagaggagac ctgagctgca tcttgcaggc
     8101 tggtaggatc tggtttggtc tgaggctaag gcactgctac ctctaatggt tgctgcacag
     8161 cctgtgtttg tagggaattc tgatggaatg gcttaagagc agaccctgct tccaaagagt
     8221 ggcaggccag gatggctcat ggaccctact gctcagccca acactgagag ggaatacaca
     8281 cactttttct gttcttggtg ctgcctgtag gggcgcttgg ccatggagtg tgttggggcg
     8341 ggaggggggg ggcagttctt agggttttac tgttgtgaac agacaccgtg accaaggcaa
     8401 atcttataaa aacatttaat tggggctggc ttacaggttc agaggttaag tccattatca
     8461 tcaaggtggg agcatggcag tatccattca gacatggcgc aggcagagct gagagttcta
     8521 cgtcttcatt caaagacttc aaggcgcata acgataccac gatatcaaca agtttgtaca
     8581 aaaaagcagg ctggcgccgg aaccgaagtt cctattccga agttcctatt ctctagaaag
     8641 tataggaact tcgaaccctt tcccacacca ccctccacac ttgccccaaa cactgccaac
     8701 tatgtaggag gaaggggttg ggactaacag aagaacccgt tgtggggaag ctgttgggag
     8761 ggtcacttta tgttcttgcc caaggtcagt tgggtggcct gcttctgatg aggtggtccc
     8821 aaggtctggg gtagaaggtg agagggacag gccaccaagg tcagcccccc ccccctatcc
     8881 cataggagcc aggtccctct cctggacagg aagactgaag gggagatgcc agagactcag
     8941 tgaagcctgg ggtaccctat tggagtcctt caaggaaaca aacttggcct caccaggcct
     9001 cagccttggc tcctcctggg aactctactg cccttgggat ccccttgtag ttgtgggtta
     9061 cataggaagg gggacgggat tccccttgac tggctagcct actcttttct tcagtcttct
     9121 ccatctcctc tcacctgtct ctcgaccctt tccctaggat agacttggaa aaagataagg
     9181 ggagaaaaca aatgcaaacg aggccagaaa gattttggct gggcattcct tccgctagct
     9241 tttattggga tcccctagtt tgtgataggc cttttagcta catctgccaa tccatctcat
     9301 tttcacacac acacacacca ctttccttct ggtcagtggg cacatgtcca gcctcaagtt
     9361 tatatcacca cccccaatgc ccaacacttg tatggccttg ggcgggtcat cccccccccc
     9421 acccccagta tctgcaacct caagctagct tgggtgcgtt ggttgtggat aagtagctag
     9481 actccagcaa ccagtaacct ctgccctttc tcctccatga caaccaggtc ccaggtcccg
     9541 aaaaccaaag aagaagaacg cagatctccg agggcagagg aagtcttcta acatgcggtg
     9601 acgtggagga gaatcccggc cctgggatct ggactctaga ggatcccgtc gttttacaac
     9661 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     9721 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     9781 gcctgaatgg cgaatggcgc tttgcctggt ttccggcacc agaagcggtg ccggaaagct
     9841 ggctggagtg cgatcttcct gaggccgata ctgtcgtcgt cccctcaaac tggcagatgc
     9901 acggttacga tgcgcccatc tacaccaacg tgacctatcc cattacggtc aatccgccgt
     9961 ttgttcccac ggagaatccg acgggttgtt actcgctcac atttaatgtt gatgaaagct
    10021 ggctacagga aggccagacg cgaattattt ttgatggcgt taactcggcg tttcatctgt
    10081 ggtgcaacgg gcgctgggtc ggttacggcc aggacagtcg tttgccgtct gaatttgacc
    10141 tgagcgcatt tttacgcgcc ggagaaaacc gcctcgcggt gatggtgctg cgctggagtg
    10201 acggcagtta tctggaagat caggatatgt ggcggatgag cggcattttc cgtgacgtct
    10261 cgttgctgca taaaccgact acacaaatca gcgatttcca tgttgccact cgctttaatg
    10321 atgatttcag ccgcgctgta ctggaggctg aagttcagat gtgcggcgag ttgcgtgact
    10381 acctacgggt aacagtttct ttatggcagg gtgaaacgca ggtcgccagc ggcaccgcgc
    10441 ctttcggcgg tgaaattatc gatgagcgtg gtggttatgc cgatcgcgtc acactacgtc
    10501 tgaacgtcga aaacccgaaa ctgtggagcg ccgaaatccc gaatctctat cgtgcggtgg
    10561 ttgaactgca caccgccgac ggcacgctga ttgaagcaga agcctgcgat gtcggtttcc
    10621 gcgaggtgcg gattgaaaat ggtctgctgc tgctgaacgg caagccgttg ctgattcgag
    10681 gcgttaaccg tcacgagcat catcctctgc atggtcaggt catggatgag cagacgatgg
    10741 tgcaggatat cctgctgatg aagcagaaca actttaacgc cgtgcgctgt tcgcattatc
    10801 cgaaccatcc gctgtggtac acgctgtgcg accgctacgg cctgtatgtg gtggatgaag
    10861 ccaatattga aacccacggc atggtgccaa tgaatcgtct gaccgatgat ccgcgctggc
    10921 taccggcgat gagcgaacgc gtaacgcgaa tggtgcagcg cgatcgtaat cacccgagtg
    10981 tgatcatctg gtcgctgggg aatgaatcag gccacggcgc taatcacgac gcgctgtatc
    11041 gctggatcaa atctgtcgat ccttcccgcc cggtgcagta tgaaggcggc ggagccgaca
    11101 ccacggccac cgatattatt tgcccgatgt acgcgcgcgt ggatgaagac cagcccttcc
    11161 cggctgtgcc gaaatggtcc atcaaaaaat ggctttcgct acctggagag acgcgcccgc
    11221 tgatcctttg cgaatacgcc cacgcgatgg gtaacagtct tggcggtttc gctaaatact
    11281 ggcaggcgtt tcgtcagtat ccccgtttac agggcggctt cgtctgggac tgggtggatc
    11341 agtcgctgat taaatatgat gaaaacggca acccgtggtc ggcttacggc ggtgattttg
    11401 gcgatacgcc gaacgatcgc cagttctgta tgaacggtct ggtctttgcc gaccgcacgc
    11461 cgcatccagc gctgacggaa gcaaaacacc agcagcagtt tttccagttc cgtttatccg
    11521 ggcaaaccat cgaagtgacc agcgaatacc tgttccgtca tagcgataac gagctcctgc
    11581 actggatggt ggcgctggat ggtaagccgc tggcaagcgg tgaagtgcct ctggatgtcg
    11641 ctccacaagg taaacagttg attgaactgc ctgaactacc gcagccggag agcgccgggc
    11701 aactctggct cacagtacgc gtagtgcaac cgaacgcgac cgcatggtca gaagccgggc
    11761 acatcagcgc ctggcagcag tggcgtctgg cggaaaacct cagtgtgacg ctccccgccg
    11821 cgtcccacgc catcccgcat ctgaccacca gcgaaatgga tttttgcatc gagctgggta
    11881 ataagcgttg gcaatttaac cgccagtcag gctttctttc acagatgtgg attggcgata
    11941 aaaaacaact gctgacgccg ctgcgcgatc agttcacccg tgcaccgctg gataacgaca
    12001 ttggcgtaag tgaagcgacc cgcattgacc ctaacgcctg ggtcgaacgc tggaaggcgg
    12061 cgggccatta ccaggccgaa gcagcgttgt tgcagtgcac ggcagataca cttgctgatg
    12121 cggtgctgat tacgaccgct cacgcgtggc agcatcaggg gaaaacctta tttatcagcc
    12181 ggaaaaccta ccggattgat ggtagtggtc aaatggcgat taccgttgat gttgaagtgg
    12241 cgagcgatac accgcatccg gcgcggattg gcctgaactg ccagctggcg caggtagcag
    12301 agcgggtaaa ctggctcgga ttagggccgc aagaaaacta tcccgaccgc cttactgccg
    12361 cctgttttga ccgctgggat ctgccattgt cagacatgta taccccgtac gtcttcccga
    12421 gcgaaaacgg tctgcgctgc gggacgcgcg aattgaatta tggcccacac cagtggcgcg
    12481 gcgacttcca gttcaacatc agccgctaca gtcaacagca actgatggaa accagccatc
    12541 gccatctgct gcacgcggaa gaaggcacat ggctgaatat cgacggtttc catatgggga
    12601 ttggtggcga cgactcctgg agcccgtcag tatcggcgga attccagctg agcgccggtc
    12661 gctaccatta ccagttggtc tggtgtcagg ggatcccccg ggagggcaga ggaagtcttc
    12721 taacatgcgg tgacgtggag gagaatcccg gccctattga acaagatgga ttgcacgcag
    12781 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
    12841 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
    12901 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
    12961 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
    13021 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
    13081 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
    13141 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
    13201 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
    13261 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg
    13321 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
    13381 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
    13441 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
    13501 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
    13561 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
    13621 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
    13681 ccagcgcggg gatctcatgc tggagttctt cgcccacccc ccggatctaa gctctagata
    13741 agtaatgatc ataatcagcc atatcacatc tgtagaggtt ttacttgctt taaaaaacct
    13801 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
    13861 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
    13921 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
    13981 ctggatccgg gggtaccgcg tcgagaagtt cctattccga agttcctatt ctctagaaag
    14041 tataggaact tcgtcgagat aacttcgtat agcatacatt atacgaagtt atgtcgagat
    14101 atctagaccc agctttcttg tacaaagtgg ttgatatctc tatagtcgca gtaggcggct
    14161 ccctggtcca aggatataca aaccatcaca gggggtcact atgaggatga agaggaacac
    14221 atagaccctg tctctgctgg agatggggct caggaggaga tgcaagtcag cccagatgga
    14281 ctggggggga gaggtttcta atggctcaga gtgtgagcca aatagtaggt gagatgccca
    14341 tgtgtgaagg tgcgactccc taacaggtac cagtaggagc tgagtgatct atgaccagcc
    14401 agggtgaggc tgagggatgg acatgagcag agaacgtggt ccaggtcctg gtgactttga
    14461 gtctctagca gccactctaa tctagcacgg ttttcctttc gccttcagcc tcctgacagc
    14521 gggcctccgc ctttaccaac gtcctctctc ccagagggtt actatgagga agccgtgccg
    14581 ctgagccctg ggaaagctcc agagtacatc acctcaagtg agtggcagct gcccacatca
    14641 gggtggttcc ttcattctca ggaatggtgg tgagccaagg cactggtgtg gaggaggcag
    14701 ggctaaacat caccacagaa atgtaggagc aatgtgtctg ggatctaagg aaggcattta
    14761 atacaggagg ggtgagatgg cgcaacgcaa ttaatgataa cttcgtatag catacattat
    14821 acgaagttat ggtctgagct cgccatcagt tcaattctct gaggtcggga gagaaactgt
    14881 tctgtagttc attggtgcac gctctaccct gtcccctcct ggcctctaca gagcacagtc
    14941 ctgcaggttg aggtttctgc agaaatgcct gttgggtgca ggcttctctt ctgtgaagag
    15001 ggcaggcttc actgaacagt ttctgctgaa gtcatgatcc accagctccc ggtgccgacc
    15061 tcagaaatcc ttcttttaaa ctgagatgca cactgatgcc caggacctcc tcttactgcc
    15121 cctgggattc agattcatta ctgatgctat tctgtcccct gagtgtcagg ccctttcacc
    15181 tctcagcagg atgtcacgtc aagctgtgga atcctgtgtt tctgtgctca gctctgaaac
    15241 tcctctcatt gtcaagtact ttctcattaa aaagagtgtc gtggaaaaag tgtcactgta
    15301 ggacacttgt tctccagtct gatgatctga attggttcac caggacccac atagtggaaa
    15361 gagcgaatca accccccaag ctgtcctcta acctccacgc atgtaccacg acacacgtgc
    15421 ctccgcaaaa tagttgttta aagcgttgta agtgtcagtc tttgtgacag gctgctaggg
    15481 cagagtagag cacgggctaa cagtgctatc cactcccctg tgatgctcac cggcttccat
    15541 accattgcca acaccgagtg tccacatgct ctggaagctg gtttcccaga gtgcattggc
    15601 ttatagcact tttcttagac ctgtggcggg ctgccagcat cttctccagg ggccaggtga
    15661 caatctcctt ctcccagggt ctggcatttc tggctcttag gtccctgttt gacagcatga
    15721 gcctctctga tggtatgctc cccccatctg cccctccagt cagagcctgc cttctgtctt
    15781 tgccttgctg tatggtatcc acacagccat aggctttggg gccctggaac catgtgtgac
    15841 ttaattcctt catgaaaatg aaggttgaaa agttcctttg aggctgccat gtaaagaggg
    15901 ggtgttaggc agatgcagac actgggatag ccacgtgctg gtgtgtagga ggggagagga
    15961 gaaagagagg ctgctgatct gaagatggca ggagcctggc ctgactgaca agttattcta
    16021 ctgcaacctc ctgtctgcat cctcagcact tcagactggc agctctcaca ctctcaggtt
    16081 accactgcct caagtgctct gtctgttact tctcttccca gccaccccag gcctgaatgt
    16141 gccattgtcc ctctgaagga tccttccctg acagtgcact ggccatgttc ctgcaatagg
    16201 agctttgcta ggtgctggct gggagagcta ccacatacaa caggatgctc tcttcccagg
    16261 gttcccagct tggcgtaaag agctgacctg tgcacacagt gaggagtctg ttggagtgtg
    16321 tacagtgtgt cacaagggcc tatgacacag tgtgctgttg gcctgctcag gctgctgcct
    16381 caaatcactc tcagctcaga ggctggaatg ctgaaattct tgggccatgc tagagttatc
    16441 gtaccagtgc tttatcttac agatggcctg tcttagaggt gtgccttctg gacatgtcct
    16501 caccccctcc cttcttcccc cctctttcct cttgtccctt tctctcacca ctcccattgg
    16561 attaggactc taccttatgg tcgtattggt aacagatttc atctgaatcg taatccaaaa
    16621 gctccatcag atgcagtcat gttagcctaa agacctcact cagcatgaga gtctcaggga
    16681 atcttgagcc catggtactg gccagaggga gccatagctt aatgctagac atgaacacac
    16741 attatcccag gagagaggag ggagatgggg cagctcgggt gtgtacagag agcccagagg
    16801 aagagggagc aggctatgtg tggagaaagt gtaagaactg gggtgtcctt gagtgagatt
    16861 tgttctgtta gagagatggg accagtcctg cccagatcca aggcaagcca gtgggaagag
    16921 gtggcctctg gtggatccca agtgtactga agggtctgtg ggtggtagtg tcaacaagaa
    16981 gactggggtg ggggtggatt taatcaaggc cagataaaga ggagcagagg actcttcacg
    17041 gacatggttc ccgagtgacc acccaaggca gggcacagtt aatgctgcca ttggggtgac
    17101 atcatcctgt tgatgtgaac gaatctagag atttaaaaaa aaatgaatct caatgtttta
    17161 gtcgttgcat ttgaagcatt ttgatgtgtt ctcagtgcct gacttgtgca agttttatcc
    17221 ccagtgccag ggcggaagcc ctttctcact gaagcctacg gtggctggaa ataaggccag
    17281 gtgaggtgag ctggagggtc ggcctctcac ctctaccctt ttctcctcca cagattatga
    17341 ctctgatgcc atgagcagtt cctatgagtc atatgatgaa gaggaagagg acgggaaggg
    17401 gaagaaaact cggcatcagt ggccgtcaga ggaggcctct atggacctgg tgaaagacgc
    17461 caagatctgt gccttcctgc tgcgcaagaa acgctttggc cagtggacca agctgctctg
    17521 cgtcatcaaa gacaccaagc tgctggtgag gagcccagtg gggctgtgcc gccattggca
    17581 gtccgaagta caggaccaca actggtacat gcagacgcta ccacagggga ggcaagcgac
    17641 ggcccagccc agctcagccc agcccgctgg cacctcttca gggagggctc atggttcccc
    17701 cacacccaag tccttccacc ccgcggggcc tgtggcttgt gtcagtggaa atgagcagtg
    17761 ttgagacttg ccagaaactg caagatagac tttctcattt gtagcgagtc agtaaattcc
    17821 tcccagccag gtcacagcag aggccggagc ctgtggaggc gcctagggga gggcagaggg
    17881 aaaggacatt cttttggaaa gtcaaaagct ttccaaaaag aagggtgggt ttttttgttt
    17941 tgttttgttt tgttttggag gcaaaacagg aaggtggagt acatccagaa gcagggcgta
    18001 gaagattcag gactctggct acaggtgctg tccccttggg ggttccaagg agaggagcct
    18061 tggaggcggc attcctgccc ccagtccccc cagctcccaa ggccacttcc ggtaaaaacc
    18121 aaggctggtc actgggtcag gagttttact gtctgctttt tgccttttgt ctttcaaatt
    18181 aacctcagga agcaattgct caaaagtgag tccttaaagg agaaaatctg ggtttctcct
    18241 ttccccacgt gatggagcag ataacactgt aagagagacc atggccccca gtgaattatg
    18301 ggagtcttgc tcgcattggc tcttagatca ctggatgtgc ttcttcttct tcttcttctt
    18361 cttcttcttc ttcttcttct tcttcttctt cttcttcttc ttcttcttct tctttttttt
    18421 tttgttcagc ctttttaaac gaagtgcata ttttttccac atgtgtggcc tgaatttctt
    18481 ccaaatatag aatatgccaa aaatgatgcc attggggatt ttaaagcatg aaaaatatta
    18541 acatagccct tctttataat ctgatgcaat cgctgcaaga tggtgatatg ctatatgcag
    18601 tgatttaaaa tgtactccac actcatgaga aagagccgtt ttatttccag tgcagacagc
    18661 gggagggctg atgcagagtc agcagtttgg ggcccaggtt cctggcccca cacccgttgg
    18721 tcctcttcct cctctcttgc ttttctctgc agtgagagcc ctctccctgc tctatgatgg
    18781 tgagagcttt catagctttc atagagccta cctttaaatt gttgaaggaa taaatggaaa
    18841 atgagggaaa acatcatgta agacaaaatg caaggtgaaa cttggccttt tatttgattt
    18901 tggctttgtt atgacagaat cttgctagat agctcaggtt agcctcaaat tcactatgta
    18961 gcccaggcta gtcttgagct ctgaatcctt tctcttccat ctcctgaatg atgagattat
    19021 gagcaagtac tacacacatt tggcttaaag tagtagtagt agtaattgtt gttttgtttg
    19081 tttgtttgtt gttgtgctgt gtgtatgtat gtatgtatgt gtgtgtatgt atgtatgtgt
    19141 gtgtgtatgt atgtatatgt atgtatgtat gtatttttat gtatatgtgt gtatgcatta
    19201 tcttccttca taattaaccc atgatcaact actcagatga gagaaagcat acttttcttt
    19261 cctggaacac cttgcctggc ctccccctgt gggtgtcacg ctacctactc tattgactcc
    19321 atctctagac cttttatttc catgcccagg caattccaga cctacccagt cacccccaga
    19381 cactctgccc actcactgaa gtatctgtct cctagctggt gggtatcact ggaagagcct
    19441 ctccactgga tggactctga ggccaagtca cctttcttcc tggggtctgc ctgtacctag
    19501 tgctgaggga gtcaaggggc agagccagcg ggcttccagc tccagggccc tgggctctct
    19561 atgaatttcg cttgaactga gtcctgcctg gtcttccttc ctcaggctct ttgcagcagc
    19621 cttgggggac tcctgtttgg aggtggcctg ggcttgcctc ccaggatctc cagacagtac
    19681 tagcattcca ggtggttgtg agccaactgc tgtgggtgct ggggacaggg cttaggctcg
    19741 ctacaagggc agtgcgcact tttaagtgct gagccatttc tccagcacca agtgtcgtta
    19801 tcattaccta gtctgcccac agtagctcta tgcagttgca acagatctag taaccctttc
    19861 atgtatgtta tattcacatg a
//