LOCUS       KO-first_condition_ready_29130_MGI:1916835        23545 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Hfe2 targeting project(s): 29130
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_Bact_P
COMMENT     design_id: 96308
COMMENT     backbone: L4L3_pD223_DTA_spec
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    complement(4153..4190)
                     /note="Transcription Terminator"
     misc_feature    3826..4079
                     /note="BGH pA"
     CDS             3045..3701
                     /note="DTA"
     promoter        2519..3031
                     /note="PGK promoter"
     promoter        complement(2368..2518)
                     /note="spectinomycin promoter"
     CDS             complement(1357..2367)
                     /translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQA
                     RYAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLL
                     AVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVE
                     VTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVAL
                     VGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIA
                     PKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVV
                     GK"
                     /note="SpecR"
     misc_feature    complement(617..1209)
                     /note="pUC ori"
     misc_feature    complement(319..346)
                     /note="rrnB T2"
     misc_feature    complement(144..187)
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            8364..8509
                     /db_xref="ENSEMBL:ENSMUSE00000332133"
                     /rank="1"
                     /note="ENSMUSE00000332133"
     exon            9608..9781
                     /db_xref="ENSEMBL:ENSMUSE00000249056"
                     /rank="2"
                     /note="ENSMUSE00000249056"
     misc_feature    4275..9963
                     /note="5 arm"
     misc_feature    9991..10015
                     /note="B1 Gateway"
     misc_feature    10028..10075
                     /note="Frt"
     intron          10076..10950
                     /note="mouse En2 intron"
     misc_feature    10948..10950
                     /note="Splice Acceptor"
     exon            10951..11123
                     /note="mouse En2 exon"
     misc_feature    11135..11728
                     /note="ECMV IRES"
     gene            11729..14789
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    14828..15071
                     /note="SV40 polyadenylation site"
     misc_feature    15075..15108
                     /note="loxP"
     promoter        15135..15654
                     /note="human beta actin promoter"
     gene            15680..16474
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    16674..16915
                     /note="SV40 polyadenylation site"
     misc_feature    16932..16979
                     /note="Frt"
     misc_feature    16986..17019
                     /note="loxP"
     misc_feature    10965..11042
                     /note="FCHK std_gpr"
     misc_feature    17035..17055
                     /note="B2 Gateway"
     misc_feature    9964..17085
                     /note="Synthetic Cassette"
     exon            17336..17883
                     /db_xref="ENSEMBL:ENSMUSE00000249046"
                     /type="targeted"
                     /rank="3"
                     /note="target exon 1 ENSMUSE00000249046"
     exon            18365..19227
                     /db_xref="ENSEMBL:ENSMUSE00000378482"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 2 ENSMUSE00000378482 5' fragment"
     misc_feature    17086..19227
                     /note="Critical Region"
     misc_feature    19251..19284
                     /note="Downstream LoxP"
     misc_feature    19228..19307
                     /note="synthetic loxP region"
     exon            19308..19597
                     /db_xref="ENSEMBL:ENSMUSE00000378482"
                     /rank="4"
                     /note="ENSMUSE00000378482 3' fragment"
     misc_feature    19308..23545
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatttaag
       61 actggccgtc gttttacaac gtcgtgactg ggaaaacatc catgctagcg ttaacgcgag
      121 agtagggaac tgccaggcat caaataaaac gaaaggctca gtcggaagac tgggcctttc
      181 gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg
      241 atttgaacgt tgtgaagcaa cggcccggag ggtggcgggc aggacgcccg ccataaactg
      301 ccaggcatca aactaagcag aaggccatcc tgacggatgg cctttttgcg tttctacaaa
      361 ctcttcctgg ctagcggtac gcgtattaat tgcgttgcgc tcactgcccg ctttccagtc
      421 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
      481 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
      541 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
      601 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
      661 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
      721 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      781 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
      841 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
      901 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
      961 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     1021 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     1081 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     1141 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     1201 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     1261 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg acgcgtaact
     1321 cacgttaagg gattttggtc atgggtggct cgagggttat ttgccgacta ccttggtgat
     1381 ctcgcctttc acgtagtgga caaattcttc caactgatct gcgcgcgagg ccaagcgatc
     1441 ttcttcttgt ccaagataag cctgtctagc ttcaagtatg acgggctgat actgggccgg
     1501 caggcgctcc attgcccagt cggcagcgac atccttcggc gcgattttgc cggttactgc
     1561 gctgtaccaa atgcgggaca acgtaagcac tacatttcgc tcatcgccag cccagtcggg
     1621 cggcgagttc catagcgtta aggtttcatt tagcgcctca aatagatcct gttcaggaac
     1681 cggatcaaag agttcctccg ccgctggacc taccaaggca acgctatgtt ctcttgcttt
     1741 tgtcagcaag atagccagat caatgtcgat cgtggctggc tcgaagatac ctgcaagaat
     1801 gtcattgcgc tgccattctc caaattgcag ttcgcgctta gctggataac gccacggaat
     1861 gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg agaatctcgc tctctccagg
     1921 ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc cgcgttgttt catcaagcct
     1981 tacggtcacc gtaaccagca aatcaatatc actgtgtggc ttcaggccgc catccactgc
     2041 ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga tggcgctcga tgacgccaac
     2101 tacctctgat agttgagtcg atacttcggc gatcaccgct tccctcataa tgtttaactt
     2161 tgttttaggg cgactgccct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg
     2221 acccacggcg taacgcgctt gctgcttgga tgcccgaggc atagactgta ccccaaaaaa
     2281 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
     2341 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagcttac gaaccgaaca
     2401 ggcttatgtc cactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac
     2461 cttgggtagc agcgaagtcg aggcatttct gtcctggctg gtctagagct gtttatcgaa
     2521 ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc
     2581 ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc
     2641 ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc
     2701 ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa
     2761 gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta
     2821 ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg
     2881 ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga
     2941 aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc
     3001 tcctcttcct catctccggg cctttcgacc tgcaggtcct cgccatggat cctgatgatg
     3061 ttgttgattc ttctaaatct tttgtgatgg aaaacttttc ttcgtaccac gggactaaac
     3121 ctggttatgt agattccatt caaaaaggta tacaaaagcc aaaatctggt acacaaggaa
     3181 attatgacga tgattggaaa gggttttata gtaccgacaa taaatacgac gctgcgggat
     3241 actctgtaga taatgaaaac ccgctctctg gaaaagctgg aggcgtggtc aaagtgacgt
     3301 atccaggact gacgaaggtt ctcgcactaa aagtggataa tgccgaaact attaagaaag
     3361 agttaggttt aagtctcact gaaccgttga tggagcaagt cggaacggaa gagtttatca
     3421 aaaggttcgg tgatggtgct tcgcgtgtag tgctcagcct tcccttcgct gaggggagtt
     3481 ctagcgttga atatattaat aactgggaac aggcgaaagc gttaagcgta gaacttgaga
     3541 ttaattttga aacccgtgga aaacgtggcc aagatgcgat gtatgagtat atggctcaag
     3601 cctgtgcagg aaatcgtgtc aggcgatctc tttgtgaagg aaccttactt ctgtggtgtg
     3661 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
     3721 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
     3781 ggaactgatg aatgggagca gtggtggaat gcagatccta gagctcgctg atcagcctcg
     3841 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     3901 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     3961 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     4021 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     4081 agaaccagct ggggctcgag atccactagt tctagcctcg aggctagagc ggccgagttt
     4141 aaacagataa acggaaaaaa accgggccaa tgttggcccg gtttttttgg atttaggtga
     4201 cactatagat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggacacggg atgccatttt taacccacat acctgaacct ctctagcctt
     4321 ccaagtgttg ggactaaaca ctccatgcag tgtgacatct tcatttattt actgagtgat
     4381 tctagaattt ttccatcagt ttaagatatg gttttcataa gaaaatcaga ataaagctag
     4441 gaatagtggc tcacacctgg aacgcaagtg tgtgggaggt ggaggcagag ggatcatcct
     4501 aaactacgtt ttaggttgga agctaacctt tgaaactttg ttttcaaaaa tacagaaaac
     4561 aaaacaacaa aaaaaaaagc tggtgaggtg gctgggtggg cataggtgct tgccaccaag
     4621 cccaacagca ttggttggat tccccggacc cacatagtag aagggcctga aagttgtcct
     4681 gtgactttca catctgcact gtgacacaca ctcactccct ccacccacaa atacataaat
     4741 aaaagtagtg aaaatttgag aacaaaaaaa aataattaac tattttttcc catttaccag
     4801 cacttctcaa aatggacgat acaacagctt tctccaatac tagccgatca ccttttactt
     4861 tattatttaa tcttttttta tagtccagtc attatcctcc tcccagtctg ccttccaaca
     4921 gttcctcacc ccagtactcc tccccatgct ttttactttt aagtataatt gtgaattgag
     4981 gcttaaaata cttggtgtgt gtcaacccaa tatactcagt ttctattttg ataactaatt
     5041 tgtcctgtct ttggagagcg acaactatct ttttgtttgc ttgcttgatt tgagacagga
     5101 cctcactatg tagccttggc tagtctggaa ctccttatgt aggctaggtt ggcctgaaat
     5161 tcatagagat gcatcgacct ctgcctccta agtgctgaga ataaaggcat gtgccaccac
     5221 gcccagcttg atacagtatt ttaaagcttc cttgtttagc ttggataatt cctggctcca
     5281 aatgggaata ggttatttct ccaaagaatt ctgatttctt gtagtgggac atcatatttc
     5341 tgttccacaa cttggacatt agaagtgttc actaccaatg atcactgctt tgaggtattt
     5401 tcataataaa ttaggaatag ttaaagacaa aatgtcatga acacacagtt atctttgtat
     5461 ttaaattttt ttttttttaa atgtgtatgg gtgttttgcc tgcatgtacg tctctgcgca
     5521 tcttcatgca atgcctgagg agaccagaag gaggctccag gtcccctgga gcagtttaga
     5581 tttgaattac gggagttttt acttatcaac atgaatcagt atttctatcc cacatcaaat
     5641 atcatggctc tctaaagtcc aagaattggc cacctgtaat tatagcaccc aggaagttga
     5701 gacaggagga tcgcaagttt caggccagcc taggctatat ggtgaaactc tgtttcaaac
     5761 aaaaccaaac caaaacccca ccattattac ttaaaaataa ggttttattt ttgaatgtgt
     5821 gtctgtgtgt gggttgagta gttcccaagg ggaccagaag agagcattga ctctcctgga
     5881 gcccctgacg tggattcggg gaaccaaact caggtccctt gtaaaaccag caggtgctct
     5941 caactgtggc tccatctctc cagccttcct taatttcttt ttaagcccac agcaaactca
     6001 aaatagttcc agaataatac caacgccact gtcaatgata gtgaggactg taaaatactt
     6061 tggtactagt tgggcacagt ggcccctgcc tataacccag caccttcagg gtaattatct
     6121 aaatcatctg gccaacataa aaggttctaa gccagctggg accacacata taacaacact
     6181 ctgtttcaaa acaactgatt ccttaaaaat atttttattt tttaaatttt atgtgcatat
     6241 gtgtatgcct gagtgtatgt gtgtatgtgc accacatgta tgtgtatgtg gcctgaatgt
     6301 atgtatgtct gtgcactaac tgtgtgcaga agccattaga ggtcaactga gggtattgga
     6361 gctcctaaac tggaattaca gttagtaatg agctatcatg tgggtgatag aagtggaacc
     6421 tgaattccct gtaggagcag caagtgcttc aaccactaaa ccatccttcc agcctcaaga
     6481 aactgttttt tttttgcacg tattttgggt ttcagagtat atgccacttt gcattttata
     6541 gacaacctag tatgtcttaa agtcacttga ttaatttagt tgggggatgt cttagtcagg
     6601 gtgtgtattc ctacacaaaa catcatgacc aagaagcaaa ttggggagga aagggtttat
     6661 tcagcttaca cttccacatt gctgttcatc accaaaggaa gtcaggactg gaactcaagc
     6721 aggtcaggaa gcaggagctg atgcagaggc catggaggga tgttacttac tggcttgctt
     6781 cccctggctt gcccagcttg ctctcttata gaactaagac taccagccca gagatggtcc
     6841 cacccacaat gggcagggct cgccccactt gatcactaat tgagaaaatg cttacagctg
     6901 gatctcatgg aggcacttcc tcaagggagg ctcctttctc tgtgataact ccagcctgtg
     6961 tcaagttgac acacccaacc atccagtgca gggaaatttt atggaataaa agttcttggg
     7021 atgtgggctg gtgaaatggc tcagcaggta ggtagaggaa ctagctacca aacctcacat
     7081 ttggccccta acgcccacat ggtgggagga aagaattgac tcctataacg tttctgacat
     7141 ccacataggt acacacagac acacagagac agacatgttc taaataactt aatgtaaaag
     7201 attattgtga tatggtcttg tatagttgag aaaaagctaa attcattata aaaaatacat
     7261 gtgctcccac acttctcttg taaagtgtgt gtgtgtgtgt gtgtgtgtgt gtgtatgcgt
     7321 gcctgagaag ggaccatgta ccatgtggac ctgtagaact tcatgtagag ctgacagggc
     7381 cagaagcttt tgtgacacac tttgtaatca gtcctgtgct gctttccctt ggcaagtact
     7441 gtgggacagt gtcccagaaa tgccaccagg tgaaaagacg gacatagcta aaaccctgat
     7501 tataattgaa tcttcaaaca gtttgtctat aaaagtatat tcccaaatgt cttccttctg
     7561 aattcaccta tctaacgcca ctcctctgtg gatcagtagt gttcaaaact gactccgatt
     7621 cttctctctg cctctgttac taccccggcc ccggcccctg caacctggaa gcttctgttt
     7681 tttgtgtttg tattgatgag gaggagggag gagggattgt tgcacaaact agtgtaatgg
     7741 aaaaggtgga gagagagtag acagctgcag agatctgatc tgggcagacg gaataaaccc
     7801 ttctcgaaac aattctgctc tcggatttct cgggacagct ggacctttca gcttctgaaa
     7861 tagttggcag agatggggga gggtctctga actatgcttg ccgtccatct tcaaagccgg
     7921 ccacctcagt gtattatgtg tagaagaaat tcaatggaat cctcagtaaa gagaggatga
     7981 gagcagtgag tgactggcat cacacaaaat caccaccagc agtcttaaag gcattactgg
     8041 gaatactgag ttcattactg ggaatgctgg agggcctgag tggcagctta taagctacag
     8101 ttggatatat gacttggcca tcatctttct ttatactctc cctcccttgg tatccaatat
     8161 ttccatattc catccttatt ttgctcatcg ttcctgctgc cttagctccc acaccccact
     8221 gccaccaacg ttcctggaat tttggaccta gctattttta aaactgtcaa ctcagtaggc
     8281 acctccctcc tcctctcagc tgtccagtac ttgggccaac catatactct ccctcccccc
     8341 tccccccaca ccaaacctcc tctggctctc tgacctgagt gagactgcag ccattccggg
     8401 gcaatcatgg agaaagagat gggggacccc ctggctggag cagaccaaca gaataggcaa
     8461 ctatggctcg agaacccagt atcagagtaa tgcttgacct cgggaaacag taagtccaga
     8521 tgaaatgtcg atcactttga taagcttttg ggtcaaggcc agaacttcat aaagttacag
     8581 acatctgttc tgaaaactaa gatctcccct tagcaactac cccagtcttc acttttcctc
     8641 ttgcctagat gttttctaat ccatggccat ttgatgttta atgctgcctc tctctggctt
     8701 tcctattttc ctctccatct tattacacca agaccttggg agagggggag ggggcagtgc
     8761 taaggactgg gcccagggcc ttgagcatgg taaacactca gctaccatcg agttacactc
     8821 ccatccctac ctcaagacct ctctgacctc ctgctcatat gcttatccca aagtagggtt
     8881 ttgacaggag aaaaggagac agaccctccc ccatatctgt tctcctcaaa agaccaggag
     8941 tgtctggatt gggcttttca gctgggtctg gaaaaacaca tgaactgtta gacagaaaca
     9001 tatattaatt tgaatgcttg atttgtaccc catcctgtga gagggatcgt actgtcagcc
     9061 ctctggagag gctgagagac cggctctcct ttcactggga aggtgatatc gtagacaggg
     9121 cgtctgccta ccatgttacc ctttgtgccc ttctaacaca gtgaagaaaa tgaaacggca
     9181 ggtggtttct ggtgggaagg atccataaga agtatgttct taaagcgtga tcagatcctg
     9241 gtgctactgg ggacccactg acatggtcag aacctcactt tctctagggg tcctccattt
     9301 ttggcacatc tgctatttgg tagtcaggat ggggcagtac actggactct gcacactgag
     9361 gtcactcaac cttccctcca ccaccacccc gcccccgtag gcatattgca caagggacct
     9421 gaaggtggcc gcaggggcag ggcacagagg ctcaccagcc acgctcaccc tctggaagcc
     9481 tccttcttcc caagatccag tctgtctcat ttttgttttg gtctcttcag atgctctctc
     9541 tacccaccat cttgactggt ctccaaccct tctctttccc cacactcagc caaatttctt
     9601 cttccagtca cagaagtacc cagagaaatt cactaggtag gaggctcatc atctgggaag
     9661 aaccggtgcc tggggggacc tggctggata ggtatgggcc agtcccctag tccccggtcc
     9721 ccccacggca gccctccaac tctaagcacc ctcactctcc tgctgctcct ctgtggacag
     9781 ggtaaggaag ggccagggaa gggttggaca tatccagagg ttaggatgct gggtaggaat
     9841 gagcgcgcgg gggtgggggt gggtgggggt gtgggggtgt ggggggtggg gggagagtga
     9901 ggagggatgt gtgctgagtc ccagcacctc ccaactctga aagaggcctg tcttgtttcc
     9961 cataaggcgc ataacgatac cacgatatca acaagtttgt acaaaaaagc aggctggcgc
    10021 cggaaccgaa gttcctattc cgaagttcct attctctaga aagtatagga acttcgaacc
    10081 ctttcccaca ccaccctcca cacttgcccc aaacactgcc aactatgtag gaggaagggg
    10141 ttgggactaa cagaagaacc cgttgtgggg aagctgttgg gagggtcact ttatgttctt
    10201 gcccaaggtc agttgggtgg cctgcttctg atgaggtggt cccaaggtct ggggtagaag
    10261 gtgagaggga caggccacca aggtcagccc ccccccccta tcccatagga gccaggtccc
    10321 tctcctggac aggaagactg aaggggagat gccagagact cagtgaagcc tggggtaccc
    10381 tattggagtc cttcaaggaa acaaacttgg cctcaccagg cctcagcctt ggctcctcct
    10441 gggaactcta ctgcccttgg gatccccttg tagttgtggg ttacatagga agggggacgg
    10501 gattcccctt gactggctag cctactcttt tcttcagtct tctccatctc ctctcacctg
    10561 tctctcgacc ctttccctag gatagacttg gaaaaagata aggggagaaa acaaatgcaa
    10621 acgaggccag aaagattttg gctgggcatt ccttccgcta gcttttattg ggatccccta
    10681 gtttgtgata ggccttttag ctacatctgc caatccatct cattttcaca cacacacaca
    10741 ccactttcct tctggtcagt gggcacatgt ccagcctcaa gtttatatca ccacccccaa
    10801 tgcccaacac ttgtatggcc ttgggcgggt catccccccc cccaccccca gtatctgcaa
    10861 cctcaagcta gcttgggtgc gttggttgtg gataagtagc tagactccag caaccagtaa
    10921 cctctgccct ttctcctcca tgacaaccag gtcccaggtc ccgaaaacca aagaagaaga
    10981 accctaacaa agaggacaag cggcctcgca cagccttcac tgctgagcag ctccagaggc
    11041 tcaaggctga gtttcagacc aacaggtacc tgacagagca gcggcgccag agtctggcac
    11101 aggagctcgg tacccggaag atctggactc tagagaattc cgcccctctc cctccccccc
    11161 ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt
    11221 attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt
    11281 cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa
    11341 tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac
    11401 cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg
    11461 tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt
    11521 tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca
    11581 gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt
    11641 ttagtcgagg ttaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga
    11701 aaaacacgat gataagcttg ccacaaccat ggaagatccc gtcgttttac aacgtcgtga
    11761 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
    11821 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
    11881 tggcgaatgg cgctttgcct ggtttccggc accagaagcg gtgccggaaa gctggctgga
    11941 gtgcgatctt cctgaggccg atactgtcgt cgtcccctca aactggcaga tgcacggtta
    12001 cgatgcgccc atctacacca acgtgaccta tcccattacg gtcaatccgc cgtttgttcc
    12061 cacggagaat ccgacgggtt gttactcgct cacatttaat gttgatgaaa gctggctaca
    12121 ggaaggccag acgcgaatta tttttgatgg cgttaactcg gcgtttcatc tgtggtgcaa
    12181 cgggcgctgg gtcggttacg gccaggacag tcgtttgccg tctgaatttg acctgagcgc
    12241 atttttacgc gccggagaaa accgcctcgc ggtgatggtg ctgcgctgga gtgacggcag
    12301 ttatctggaa gatcaggata tgtggcggat gagcggcatt ttccgtgacg tctcgttgct
    12361 gcataaaccg actacacaaa tcagcgattt ccatgttgcc actcgcttta atgatgattt
    12421 cagccgcgct gtactggagg ctgaagttca gatgtgcggc gagttgcgtg actacctacg
    12481 ggtaacagtt tctttatggc agggtgaaac gcaggtcgcc agcggcaccg cgcctttcgg
    12541 cggtgaaatt atcgatgagc gtggtggtta tgccgatcgc gtcacactac gtctgaacgt
    12601 cgaaaacccg aaactgtgga gcgccgaaat cccgaatctc tatcgtgcgg tggttgaact
    12661 gcacaccgcc gacggcacgc tgattgaagc agaagcctgc gatgtcggtt tccgcgaggt
    12721 gcggattgaa aatggtctgc tgctgctgaa cggcaagccg ttgctgattc gaggcgttaa
    12781 ccgtcacgag catcatcctc tgcatggtca ggtcatggat gagcagacga tggtgcagga
    12841 tatcctgctg atgaagcaga acaactttaa cgccgtgcgc tgttcgcatt atccgaacca
    12901 tccgctgtgg tacacgctgt gcgaccgcta cggcctgtat gtggtggatg aagccaatat
    12961 tgaaacccac ggcatggtgc caatgaatcg tctgaccgat gatccgcgct ggctaccggc
    13021 gatgagcgaa cgcgtaacgc gaatggtgca gcgcgatcgt aatcacccga gtgtgatcat
    13081 ctggtcgctg gggaatgaat caggccacgg cgctaatcac gacgcgctgt atcgctggat
    13141 caaatctgtc gatccttccc gcccggtgca gtatgaaggc ggcggagccg acaccacggc
    13201 caccgatatt atttgcccga tgtacgcgcg cgtggatgaa gaccagccct tcccggctgt
    13261 gccgaaatgg tccatcaaaa aatggctttc gctacctgga gagacgcgcc cgctgatcct
    13321 ttgcgaatac gcccacgcga tgggtaacag tcttggcggt ttcgctaaat actggcaggc
    13381 gtttcgtcag tatccccgtt tacagggcgg cttcgtctgg gactgggtgg atcagtcgct
    13441 gattaaatat gatgaaaacg gcaacccgtg gtcggcttac ggcggtgatt ttggcgatac
    13501 gccgaacgat cgccagttct gtatgaacgg tctggtcttt gccgaccgca cgccgcatcc
    13561 agcgctgacg gaagcaaaac accagcagca gtttttccag ttccgtttat ccgggcaaac
    13621 catcgaagtg accagcgaat acctgttccg tcatagcgat aacgagctcc tgcactggat
    13681 ggtggcgctg gatggtaagc cgctggcaag cggtgaagtg cctctggatg tcgctccaca
    13741 aggtaaacag ttgattgaac tgcctgaact accgcagccg gagagcgccg ggcaactctg
    13801 gctcacagta cgcgtagtgc aaccgaacgc gaccgcatgg tcagaagccg ggcacatcag
    13861 cgcctggcag cagtggcgtc tggcggaaaa cctcagtgtg acgctccccg ccgcgtccca
    13921 cgccatcccg catctgacca ccagcgaaat ggatttttgc atcgagctgg gtaataagcg
    13981 ttggcaattt aaccgccagt caggctttct ttcacagatg tggattggcg ataaaaaaca
    14041 actgctgacg ccgctgcgcg atcagttcac ccgtgcaccg ctggataacg acattggcgt
    14101 aagtgaagcg acccgcattg accctaacgc ctgggtcgaa cgctggaagg cggcgggcca
    14161 ttaccaggcc gaagcagcgt tgttgcagtg cacggcagat acacttgctg atgcggtgct
    14221 gattacgacc gctcacgcgt ggcagcatca ggggaaaacc ttatttatca gccggaaaac
    14281 ctaccggatt gatggtagtg gtcaaatggc gattaccgtt gatgttgaag tggcgagcga
    14341 tacaccgcat ccggcgcgga ttggcctgaa ctgccagctg gcgcaggtag cagagcgggt
    14401 aaactggctc ggattagggc cgcaagaaaa ctatcccgac cgccttactg ccgcctgttt
    14461 tgaccgctgg gatctgccat tgtcagacat gtataccccg tacgtcttcc cgagcgaaaa
    14521 cggtctgcgc tgcgggacgc gcgaattgaa ttatggccca caccagtggc gcggcgactt
    14581 ccagttcaac atcagccgct acagtcaaca gcaactgatg gaaaccagcc atcgccatct
    14641 gctgcacgcg gaagaaggca catggctgaa tatcgacggt ttccatatgg ggattggtgg
    14701 cgacgactcc tggagcccgt cagtatcggc ggaattccag ctgagcgccg gtcgctacca
    14761 ttaccagttg gtctggtgtc aaaaataata ataaccgggc aggggggatc taagctctag
    14821 ataagtaatg atcataatca gccatatcac atctgtagag gttttacttg ctttaaaaaa
    14881 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
    14941 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
    15001 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
    15061 tgtctggatc cggaataact tcgtatagca tacattatac gaagttatgt ttaaacggcg
    15121 cgccccggaa ttcgccttct gcaggagcgt acagaaccca gggccctggc acccgtgcag
    15181 accctggccc accccacctg ggcgctcagt gcccaagaga tgtccacacc taggatgtcc
    15241 cgcggtgggt ggggggcccg agagacgggc aggccggggg caggcctggc catgcggggc
    15301 cgaaccgggc actgcccagc gtgggcgcgg gggccacggc gcgcgccccc agcccccggg
    15361 cccagcaccc caaggcggcc aacgccaaaa ctctccctcc tcctcttcct caatctcgct
    15421 ctcgctcttt ttttttttcg caaaaggagg ggagaggggg taaaaaaatg ctgcactgtg
    15481 cggcgaagcc ggtgagtgag cggcgcgggg ccaatcagcg tgcgccgttc cgaaagttgc
    15541 cttttatggc tcgagcggcc gcggcggcgc cctataaaac ccagcggcgc gacgcgccac
    15601 caccgccgag accgcgtccg ccccgcgagc acagagcctc gcctttgccg atcctctaga
    15661 gtcgagatcc gccgccacca tgattgaaca agatggattg cacgcaggtt ctccggccgc
    15721 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
    15781 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
    15841 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
    15901 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
    15961 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
    16021 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
    16081 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
    16141 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
    16201 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
    16261 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
    16321 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
    16381 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
    16441 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc
    16501 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa
    16561 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
    16621 ctcatgctgg agttcttcgc ccaccccccg gatctaagct ctagataagt aatgatcata
    16681 atcagccata tcacatctgt agaggtttta cttgctttaa aaaacctccc acacctcccc
    16741 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
    16801 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
    16861 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccggggg
    16921 taccgcgtcg agaagttcct attccgaagt tcctattctc tagaaagtat aggaacttcg
    16981 tcgagataac ttcgtatagc atacattata cgaagttatg tcgagatatc tagacccagc
    17041 tttcttgtac aaagtggttg atatctctat agtcgcagta ggcggttaac ttgaagaagg
    17101 acacagatgg cgtccttaga ccccagggaa aagaagtctg actgctttac acgtttttag
    17161 tagaggaaag gagggaagga ggcaaggatt gggagaatga aggttgagat tgggaaagcg
    17221 gccacgctga gtggtaggaa cggtgggatg gcggatggtt tttggcagtt aggtgcatac
    17281 tggacttgga tagaacctgt gtctcatttc actgccctct cccttccgtc cccagctcac
    17341 tcccagtgca agatcctccg ctgcaatgcc gagtatgtct cgtccactct gagtcttcgg
    17401 ggaggtggct caccggacac gccgcgtgga ggcggccgtg gtgggctggc ctcaggtggc
    17461 ttgtgtcgcg ccctgcgctc ctacgctctc tgcacgcggc gcacggcccg cacctgccgc
    17521 ggggaccttg ctttccactc tgcggtgcat ggcatagagg acctgatgat ccagcacaac
    17581 tgctcacgcc agggtcccac ggccccgccc ccggcccggg gccccgccct gcccggggcc
    17641 gggccagcgc ccctgacccc agatccctgt gactatgagg cccggttttc caggctgcac
    17701 ggtcgagccc cgggcttctt gcattgcgca tcctttggag atccccatgt gcgcagtttc
    17761 cacaaccaat ttcacacatg ccgtgtccaa ggagcttggc ccttgctaga taacgacttc
    17821 ctctttgtcc aggccaccag ctccccggtt tcgtcgggag ccaacgctac caccatccgg
    17881 aaggtcagag attcgatttt catcccaaca caatttcttg agccctctca cggtctgtcc
    17941 gccataccac tccttcttct tcccactatt cagccaccca gctccctgtt cttgtctttc
    18001 ccaaacattc ccttccatcc tgaatggctt ccttccatca aacatttgct cttgtaaatg
    18061 taattcagga atttctttca aagcaagtaa cttgggagtt gctgagactg agacaaaaag
    18121 gagagagaga gagggaggga gagggagaga ggcagataga gagaggcata gggagagaga
    18181 tattaggagg ttgagtgggg gtcaggcact attaatggaa cacagtaggg gataggatca
    18241 aggactgagg acccggaaga aactcgcatt tctgctggga tcggatccct gactctctat
    18301 gcagcttgat tggcgtgagg atgctttggg ccctaatgaa gtattttctc tcttgtcctc
    18361 atagatcact atcatattta aaaacatgca ggaatgcatt gaccagaaag tctaccaggc
    18421 tgaggtggac aatcttcctg cagcctttga agatggttct atcaatgggg gcgaccgacc
    18481 tgggggctcg agtttgtcca ttcaaactgc taaccttggg agtcacgtgg agattcgagc
    18541 tgcctacatt ggaacaacta tcatcattcg acagacagct gggcagctct ccttctccat
    18601 cagggtagca gaggatgtgg cgcgggcctt ctccgcagag caggacctac agctgtgtgt
    18661 tgggggatgc cctccgagcc agcgactctc tcgctcagag cgcaaccgcc gtggggctat
    18721 agccatagat actgccagaa ggctgtgtaa ggaagggctt ccggttgaag atgcctactt
    18781 ccaatcctgc gtctttgatg tttcagtctc cggtgacccc aactttactg tggcagctca
    18841 gacagctctg gacgatgccc gaatcttctt gacggattta gagaacttac atctctttcc
    18901 ctcagatgcg gggcctcccc tctctcctgc catctgccta gtcccgcttc tttcggccct
    18961 ctttgttctg tggctttgct tcagtaagta ggccagcaac ccatgactgg tttggaaacg
    19021 atttgaggat agaggttggt gtgagaaacc acaaagatgt gccaaaggaa acagcgggga
    19081 caggagacaa cacttactca atcagatgag gttgcagtcc agggctgaaa tgaccctaga
    19141 ataaagattc tgggccaggg ttttgcactc cagaccttgg tgtgggctat tcaccatgga
    19201 tttcccagtt agtgatttcc cacttgtgag atggcgcaac gcaattaatg ataacttcgt
    19261 atagcataca ttatacgaag ttatggtctg agctcgccat cagttcaaat gaaattccac
    19321 tctccataca cctgatacca ctcctacaag cctagagatt gtgagagtgc taatgaccag
    19381 tgaaacatta aaggactgag atatcgtaaa ggcaaaaaca tgattctctt tgagaaagtc
    19441 aaaagaggag aagctaatta ggaaaagctt ttggttcaga aacgaagtgg gcattgtctg
    19501 gcagaggaag tcagcttttg gagactggca ccaactcaga aacgggcatt tccatccctt
    19561 cctaatctgt tattaaagcg attagttctc catcctgtcc tctgttgcct cttttaaatc
    19621 cctttgcaga tgtggcatgg agttgaggct gtgtgttcac cccactgaaa gaggccttgc
    19681 agttaccagc tattgtctcc tccctcttgg agtctacagg ttgttggaga gaaaaaaaac
    19741 tggttcatag gaaggagtca tgagtattat tacatttgtt ttttattttc gggggagcct
    19801 catgtagtcc aggctggcct tgcattgctg tccaactgtc tctactcctc aagcgctggg
    19861 gttgaagtgg gtggaggagt gtgtaattct tcactggatt ctatggcttt tggatttctg
    19921 acagaggatt caggttctgt tcccagcgcc cacaatacta acaatggctt gcaattatag
    19981 ttccagggaa cctgatacct tcaggcctcc tctggcattt gtgtgcacat actgcacata
    20041 aattcatgct gaacaaacac acatacatgt aaaaaattta aaatatatca agtttttttg
    20101 cttcaccact ttgtgctccc agatcgcatg cttgcatcca cccaggaact atctttgggt
    20161 cctcaaatga aagacacaca cattagctta tttttaaaat gccaaaaaaa aaaagaaaaa
    20221 aaaagttaaa tgaattgaaa aaaaaatgcc ttgactatgt caaaggctgg acactcctaa
    20281 accttcccct gctatctcag gcccaatcgg ggaagtggcc agtggccaca cggctggttg
    20341 gctttatctc ctgctgctcc tgcatcccat cttctttccc tgagtgaggt ggttctctcc
    20401 tgttcctctc tgtgtcctca cctgcgcaac aaggctctcc caccccatac ccaatcattg
    20461 gctgttggca tctttattaa ttgatcaaaa accacctggg gacaaggacc tttagcattt
    20521 ggacatgcag attcctgatt gaatcaaagc attagaacca atctccagca ccaaacacac
    20581 acacacacac acacacacac acaccttatc tttatgttgg tggtgctggg gggtttctgt
    20641 gcattgggga tgaacctggg cctgtgtata ctgtctaagc actgtatatc tcatttctac
    20701 tgtaaggaag gtgtggtaca gaaagattga gcatctgtcc cacagacaca cagctaataa
    20761 gtagcagagt cagacagtga acagacaccg tttgcatctg aagatgtaac tcttagccag
    20821 ccagcgcatt cctgaggaaa gagagtgaca aggggctgtg attgtggagc aggcagaggc
    20881 aggggattgc ggcgacagat ggcactacga aagctctgct cttttctcac gtgaagccaa
    20941 agaccttcaa agtgttggga aagggaaaca agcccaaagc caccgacctt gtggaggata
    21001 aaactaaggt cagaaggtgt gctatttggg gtgttctaca taggcactgc ttctaaccaa
    21061 acaaggagag tgattccaca cttggtgcct ggcgcccctc tcctgcaggc tctcagtgcc
    21121 tggctcctct gcagttctca actcctcttt gacacctctc tagcaactcg gaccattccc
    21181 agagcacagt ctttatattt tctgaatcaa cctttagctc atctaggaaa taatctattc
    21241 caatggggac gtttctgtgt gtgtgtgtct ctctgtctct gtctctatct ctgtctctct
    21301 ctctctctgt gtctgtgtgt gcgtgtgtct gtgtgtgcct ctgtgtgcgt ctgtgtgtat
    21361 ctgtgtgtgt actgtattta ctcctgtacc ttaacttata gatactgtgg gcactttaat
    21421 gacatttgtt tagttttgtt ttttacagcg tcttatgtag gctgagctgg gcctgaactt
    21481 gctactgctt ccattttcca agttttggga ttggcctgca ccaccatgca atttattcag
    21541 tactggggac cgaactccag gcttcgtgca tgctagacaa gtacggtgcc aaccgaactg
    21601 catctccagc cttgccaaca tgttagaaca aaaatcgctc attcttaaag agcaacttta
    21661 ttcctcagag gtgggactct caaagaaaaa cttcataacc aattggctaa tgtcattgtt
    21721 ttatggcatc agtcatataa aactcttcct tgctgagact cgggatttag ccctttgccc
    21781 catggaatgt ttaaaactga gtagctcaaa tgaacacaag aaaagactac cagcattatt
    21841 agtaataggg tgatgcaaat ctaactaaaa ctttgatgag aaaagctggg gagatagtca
    21901 gtgggtaaag tgtttgtctc gtgaggatct gagcttgggt cccccgcacc cacttaaagg
    21961 ttgcacacag tgattcatgt ctgtaacttc cttccgtctg gcggactgag acaggcagat
    22021 ctctggggct tactggtcaa ccagtgtagc agaaatggtg actttcaggc tcagtcttaa
    22081 aaaataaggt ggagggccag tgagatgttc aatgggccca cgtatgagtg tggtgggaaa
    22141 gcctgggctt ctaaaattcc ttcccaggag agaccaagtg caggttaccc ctttccccta
    22201 aggtttcact gacagacaaa ccaaggcacg gttctgcaaa agttcactct ggggagccag
    22261 tgagtctatt gggctttctt acagagcaca gataacttag gaagttaaca tgcaggagca
    22321 cgggcacccc tcaaacctta cccagcaggg atcagaggag cctccctccc caagcttttc
    22381 ccagcctctc tacactctag cccaatgtct ctcaccttcc gaatgctgca acctttaata
    22441 cagttcctcg tggtatggtg acccccaacc ataaagttat tttattgcta ctttcataat
    22501 tgtaattttg ctgctattat gaattatagt ataaataaat atctgatgtt tgggttatct
    22561 gatacgtgac ccagaagtgg gtcaccagcc acaagttgag aaccacggct ctagccccac
    22621 cgtgaggcca agtgtacagc tgccaggtgg cagctaaata atcagacaag agattctccc
    22681 actcagctcc tctacgtggg ggatttcgct gggggtgggg gtgtaaactg ccaacaaccc
    22741 cattgggatg accttttgca agggggtgca gctgaactcc ctaggacggc agctgcctgg
    22801 ctgcaaaacc agcactcagg acagactgac aacttgatcc tgaagtctgc aaaggtggag
    22861 ggagagaagc aactcaacaa aactgtcccc tgacctccac atctgggatc tgccagatgt
    22921 gtgccatcgt gagcatggct acaagctcac aatacctgat aagatggaga gccaagctgg
    22981 agagatgctt agcagcgagg agcacgtgtt gctctcgcag ggacccagct cagtctccag
    23041 cacatacacg gctgctaaca cctgtctata acatccattc caaaggatcc catgccatcc
    23101 ttggcctctg agggccctgc acataagtgg ggtacagaca tgtatacagg caaaacaccc
    23161 gtaacataaa ataaagcttt taaaaaaaac tttaaaaatg gaaaattaaa gctagaaata
    23221 acaagtgtgg tgacccagtg agaaagtcac ctgccatcaa cccctggctt cctcatgtga
    23281 gggcagtggc ggcccacatg gtcatcccca gcacggggtg gggtggggag tagggtaggg
    23341 tggggggtgg ggggaagagg taggtagatc tctaggactt tgagaccagt ctggcctaca
    23401 tcctacatag tgagttccag gtcagccagg ggtatatagt gaggtcttgt ctaaaacaaa
    23461 aaacaaacaa aacaaaacaa aaaaaacaag caagcaaacg aaaccagtac aaactcaaaa
    23521 atttaataga attctgacaa tcttt
//