LOCUS       KO-first_condition_ready_36532_MGI:1915598        21666 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Serpina1f targeting project(s): 36532
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_Bact_P
COMMENT     design_id: 45786
COMMENT     backbone: L4L3_pD223_DTA_spec
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    complement(4153..4190)
                     /note="Transcription Terminator"
     misc_feature    3826..4079
                     /note="BGH pA"
     CDS             3045..3701
                     /note="DTA"
     promoter        2519..3031
                     /note="PGK promoter"
     promoter        complement(2368..2518)
                     /note="spectinomycin promoter"
     CDS             complement(1357..2367)
                     /translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQA
                     RYAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLL
                     AVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVE
                     VTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVAL
                     VGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIA
                     PKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVV
                     GK"
                     /note="SpecR"
     misc_feature    complement(617..1209)
                     /note="pUC ori"
     misc_feature    complement(319..346)
                     /note="rrnB T2"
     misc_feature    complement(144..187)
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            7187..7815
                     /db_xref="ENSEMBL:ENSMUSE00000363337"
                     /rank="3"
                     /note="ENSMUSE00000363337"
     exon            6520..6612
                     /db_xref="ENSEMBL:ENSMUSE00000406977"
                     /rank="2"
                     /note="ENSMUSE00000406977"
     exon            5680..5819
                     /db_xref="ENSEMBL:ENSMUSE00000610125"
                     /rank="1"
                     /note="ENSMUSE00000610125"
     misc_feature    4275..8760
                     /note="5 arm"
     misc_feature    8788..8812
                     /note="B1 Gateway"
     misc_feature    8825..8872
                     /note="Frt"
     intron          8873..9747
                     /note="mouse En2 intron"
     misc_feature    9745..9747
                     /note="Splice Acceptor"
     exon            9748..9920
                     /note="mouse En2 exon"
     misc_feature    9932..10525
                     /note="ECMV IRES"
     gene            10526..13586
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    13625..13868
                     /note="SV40 polyadenylation site"
     misc_feature    13872..13905
                     /note="loxP"
     promoter        13932..14451
                     /note="human beta actin promoter"
     gene            14477..15271
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    15471..15712
                     /note="SV40 polyadenylation site"
     misc_feature    15729..15776
                     /note="Frt"
     misc_feature    15783..15816
                     /note="loxP"
     misc_feature    9762..9839
                     /note="FCHK std_gpr"
     misc_feature    15832..15852
                     /note="B2 Gateway"
     misc_feature    8761..15882
                     /note="Synthetic Cassette"
     exon            16298..16571
                     /db_xref="ENSEMBL:ENSMUSE00000114445"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 1 ENSMUSE00000114445"
     misc_feature    15883..16772
                     /note="Critical Region"
     misc_feature    16796..16829
                     /note="Downstream LoxP"
     misc_feature    16773..16852
                     /note="synthetic loxP region"
     exon            18415..18702
                     /db_xref="ENSEMBL:ENSMUSE00000740774"
                     /rank="6"
                     /note="ENSMUSE00000740774"
     exon            17426..17570
                     /db_xref="ENSEMBL:ENSMUSE00000114444"
                     /rank="5"
                     /note="ENSMUSE00000114444"
     misc_feature    16853..21666
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatttaag
       61 actggccgtc gttttacaac gtcgtgactg ggaaaacatc catgctagcg ttaacgcgag
      121 agtagggaac tgccaggcat caaataaaac gaaaggctca gtcggaagac tgggcctttc
      181 gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg
      241 atttgaacgt tgtgaagcaa cggcccggag ggtggcgggc aggacgcccg ccataaactg
      301 ccaggcatca aactaagcag aaggccatcc tgacggatgg cctttttgcg tttctacaaa
      361 ctcttcctgg ctagcggtac gcgtattaat tgcgttgcgc tcactgcccg ctttccagtc
      421 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
      481 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
      541 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
      601 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
      661 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
      721 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      781 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
      841 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
      901 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
      961 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     1021 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     1081 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     1141 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     1201 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     1261 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg acgcgtaact
     1321 cacgttaagg gattttggtc atgggtggct cgagggttat ttgccgacta ccttggtgat
     1381 ctcgcctttc acgtagtgga caaattcttc caactgatct gcgcgcgagg ccaagcgatc
     1441 ttcttcttgt ccaagataag cctgtctagc ttcaagtatg acgggctgat actgggccgg
     1501 caggcgctcc attgcccagt cggcagcgac atccttcggc gcgattttgc cggttactgc
     1561 gctgtaccaa atgcgggaca acgtaagcac tacatttcgc tcatcgccag cccagtcggg
     1621 cggcgagttc catagcgtta aggtttcatt tagcgcctca aatagatcct gttcaggaac
     1681 cggatcaaag agttcctccg ccgctggacc taccaaggca acgctatgtt ctcttgcttt
     1741 tgtcagcaag atagccagat caatgtcgat cgtggctggc tcgaagatac ctgcaagaat
     1801 gtcattgcgc tgccattctc caaattgcag ttcgcgctta gctggataac gccacggaat
     1861 gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg agaatctcgc tctctccagg
     1921 ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc cgcgttgttt catcaagcct
     1981 tacggtcacc gtaaccagca aatcaatatc actgtgtggc ttcaggccgc catccactgc
     2041 ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga tggcgctcga tgacgccaac
     2101 tacctctgat agttgagtcg atacttcggc gatcaccgct tccctcataa tgtttaactt
     2161 tgttttaggg cgactgccct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg
     2221 acccacggcg taacgcgctt gctgcttgga tgcccgaggc atagactgta ccccaaaaaa
     2281 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
     2341 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagcttac gaaccgaaca
     2401 ggcttatgtc cactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac
     2461 cttgggtagc agcgaagtcg aggcatttct gtcctggctg gtctagagct gtttatcgaa
     2521 ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc
     2581 ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc
     2641 ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc
     2701 ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa
     2761 gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta
     2821 ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg
     2881 ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga
     2941 aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc
     3001 tcctcttcct catctccggg cctttcgacc tgcaggtcct cgccatggat cctgatgatg
     3061 ttgttgattc ttctaaatct tttgtgatgg aaaacttttc ttcgtaccac gggactaaac
     3121 ctggttatgt agattccatt caaaaaggta tacaaaagcc aaaatctggt acacaaggaa
     3181 attatgacga tgattggaaa gggttttata gtaccgacaa taaatacgac gctgcgggat
     3241 actctgtaga taatgaaaac ccgctctctg gaaaagctgg aggcgtggtc aaagtgacgt
     3301 atccaggact gacgaaggtt ctcgcactaa aagtggataa tgccgaaact attaagaaag
     3361 agttaggttt aagtctcact gaaccgttga tggagcaagt cggaacggaa gagtttatca
     3421 aaaggttcgg tgatggtgct tcgcgtgtag tgctcagcct tcccttcgct gaggggagtt
     3481 ctagcgttga atatattaat aactgggaac aggcgaaagc gttaagcgta gaacttgaga
     3541 ttaattttga aacccgtgga aaacgtggcc aagatgcgat gtatgagtat atggctcaag
     3601 cctgtgcagg aaatcgtgtc aggcgatctc tttgtgaagg aaccttactt ctgtggtgtg
     3661 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
     3721 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
     3781 ggaactgatg aatgggagca gtggtggaat gcagatccta gagctcgctg atcagcctcg
     3841 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     3901 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     3961 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     4021 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     4081 agaaccagct ggggctcgag atccactagt tctagcctcg aggctagagc ggccgagttt
     4141 aaacagataa acggaaaaaa accgggccaa tgttggcccg gtttttttgg atttaggtga
     4201 cactatagat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggatacagt gaccacgtac tagcaaagac ataaatagtt aggcatatat
     4321 tttaaattag ctttccttaa tctgaataga cctttgtaac cttgaaacct catcatcata
     4381 taaagaatat tttagaatat tttaagccag gtttgaatca cacacacaca ggatgttata
     4441 aaaaaaatgt aactttgaat ttctaacatc agcacacaaa aatccatacc aacttaaact
     4501 atgagattgt tttgcttttt agtctaaagg agacacaata ataaacagag ttcattggga
     4561 ctaaggcatt ttataattat tgctatggac ccagtgactg accttctcca actaggaagt
     4621 tcctcacttc ccccaaaatg gtgtcatatg actccatcca cagatcaggc caaagatgaa
     4681 gtcaaagccc tcatgatgta atcagctcta gaaaaaccct cagacacacc actagctgtc
     4741 tgagccccgc caattcccta gggctttcaa agcccaatca agctgaccgt cagaaccaac
     4801 caacacaccc catgctccag caatgtggtc tctcctttat cgcagaagga atgagaggta
     4861 tgttcacact cctgctcagg acagcatggt gcacacaagt tgaaataaac ccctaagcaa
     4921 gcatccaaag gaatattgtc catttgtttt aaaaggccga gaactgtgtc ttcacggagt
     4981 gagtgtggag tatgcggttt accaggtgac ggcctctgca gcctcaagac cctcgcaggc
     5041 ctgccagaca cagtatggta aaggttagag aggcaggaag tgctgcttgc atgtatgctt
     5101 tacatctggg tctgggactg ggtctgtggc cctgtgtgtg tattttaacg tgtgcatctg
     5161 gaagggtatt atgtgggtta cagggatcat atgtacacac gaggatgaag ggattttatc
     5221 ctctctgtcc tgaaggcaaa gccaattctc cttggagtgg acaccaacac ttttccctct
     5281 gctacaccga agacctctag acctcgtctg aagctccggc tattatccca gtctgtccta
     5341 gacaaccagg cttgaatgca atgcagcaca ggttctagta cacgcgtgca caagacttcc
     5401 caggttggga gtgggactga tatcaggcag gtaatgatta ctgaaaatac caaggcttgg
     5461 catatgtatg aactcatgac ctcctttcag caactcccag aggtaggctg caatagccac
     5521 agggctccct ccgtctccct tcaaaaaggt tggcactgga cagttctgct tcactaagtc
     5581 agttcagaga aagataacca ggtgcttagg tcacagcaac acacggcaca ccatcttcta
     5641 gtcaggatta caagctgccc aaagctctcc tccagcgtga aatgtcatca tcacacacag
     5701 ggacggagcg tgccccacgc tctgctatgc ctataagtcc ccagggtgac agctacaggg
     5761 gaagtcactg ctgcatgatc agcccacaag cttctcactg cctccacgga cgctactcag
     5821 taagtccttc accagcctcc ttctgagccc atgggctggg cactgccaac cttactacct
     5881 gtctccatac atcctccaca cagaagaagg agaggcagag attcaactcc tgagggcaaa
     5941 cccagaggag aacaaagacc ttgttctggg atcatctgag cacacagaat acccactact
     6001 gaacactggg tcgggatgcc tggttgtttt ccctccttga ggaggtcctg ggctggctct
     6061 gggtctctta cctttgtgtg tacaccttta actggggtgg aggggactaa ggagacacct
     6121 ccggtagtcc tatccatctc tctcattccc acaccccata tatgttcatc ttggacatcc
     6181 ttccataccc agtcccatgc ccctctaccc aaagtcatag acctcaacct ctgcttgtga
     6241 attcaaggac ctacacagct ccacacacaa ctctcttaaa ggagaaaaat gggctgcttc
     6301 ttcttctttt tttttttgta aaaaaaaaaa agctgctacc cttaaaggtg tgtttcccaa
     6361 gactttctat ggacagccca ggaccctagg cctgcctgcc ccagggtcat ctgcccttga
     6421 tcacctggga agaacttctg atctacaagg gaagatatgc tcattaattc ttcactgagc
     6481 tgtcgccctt ccaactcctc caccctcttt gtcatttagg ctcctgctct gattctgacc
     6541 tcacagtact ccggctggtc ctagaggctg aagcttggca aacctacaaa cttcccccaa
     6601 gatcatttcc aggtaccagt aaccatatct atatttatgt aactaatccc attatgtttc
     6661 tgcttgtaaa atgaagctca aaaattgatc tttattacag ggaaaattga tctttattac
     6721 aggtcacatg ggttctggtg acacggcttt ttgttaaatg gggcagtttc tcttgcattg
     6781 gattattgtt gatgggagtg gagtaggacc caggcagcct gctatgtatg ggacctggct
     6841 ctgccaaaat tcctgctcat ggaccttgag catattcttt aacctttccg ccttattatg
     6901 ttctcatcat aataagtgga aaactgtgta gctccacagc tgggactgag acaggaaggc
     6961 aaagtacttt tatatatatc agggaaacac agaccaggaa gacatgacct gccctgagag
     7021 gtatcactgg gtctttcaat aatgaagcta tagaaagtgc cgggcccagg ttgggcacac
     7081 agcacaggaa taggaaactg tggttgcatt tacacatgcc aatcattggt tagtggaagt
     7141 tgtcatggct ctgactctgg gctgtgttct tccctctgtc ctgcaggatg acaacaccct
     7201 tttcctccca tggactcctc ctgctagtgg gcttgtgctg tctgcttctc ataaccaaga
     7261 ctaaacatga aaaactctat gaagacccaa gcattgatcc cttccagtgc cggaaggtgg
     7321 ctcttaccat ctgcaatgtc tccatcacct tgttcaaaaa gatggcccaa ctgtcaggaa
     7381 atggaaatat tttattctcc ccgattagag tcattgctgc catttcaatg ctgtccctgg
     7441 gatccaatgg caacttaagc aaacacatcc tggagactct aaggttcaac aaaacaggct
     7501 tgcctgaagc ggagatccac aagtgtttct ggtatctgct ccattccatc caccagactg
     7561 aagagccatc atcactacaa actggtagca gcgtgttcat ccaccaggac ctgacatcag
     7621 tagacaaatt tgtgaaagga gtcaaggacc tataccattc tgacatgatc tccatcaact
     7681 ttacagacag cagtcaggcc aagacacaga tcaacaatta cgtgatggag aaaagccaga
     7741 aggaaatagt gaacattgtc aagaacctgg agagtgatac attcctggct gtggtgaact
     7801 acattatctg gaatggtaag ggtgtgtcac ttggctgagc tggctccagg ggatttatcc
     7861 aattagatgc tcacattcca atgttctgtc tccttggaat cctaaaatgt aggaaggtag
     7921 gatgcatgca tgtatcttct tcagtctttc accattaaac agtacaacac tttccagcag
     7981 tcagtaagtt tggaggatgt atttaagtaa cccctaggct gggcttgatg gatcattcta
     8041 tatacaaatg ctcttttgaa gtctgtgtgc gtgaaacaag agactatgta gtattttcta
     8101 aaaacaaatg atagatcagt atacataggg ctgtttccgt aggatacgaa acagtaagca
     8161 ccgtagagag ggtcagcttt ggagtctgaa aacattggct aatgtggcct ccatcattca
     8221 tttcctgtct acactagaca agccatttta tctgtctgtg gtatgactgt ccatttcaca
     8281 aaggtagccc atggccctga ggtaatgaaa gaatctgtga ggttgcacag tgcatggaac
     8341 ttggtggagc acatacactt gatcaactaa acttgacccc aagcatgacc aggtcctgct
     8401 tggaactggt aaggtcagga gcagctgttc ccccaaagag tctacagcct gggtaagaat
     8461 tcaaggctaa gggctgtacc tcttcaccag agctcccttc tcctgggcaa agtcctgcca
     8521 ggcacagcac tcagaccaca gattcattcc cttcccatga tcccttaatt ttccaatgag
     8581 aacatggggt aagggtctat gaggaagtct actcattttg atgacctcaa tgatgggctc
     8641 ccaaggcacc tacctactct gatgaccaaa atggtcatca tcttatacag tatcagggag
     8701 gctaccaagg catatgcact gtatctgtgg gctcaggaaa gggctcaagg tgccataaag
     8761 aaggcgcata acgataccac gatatcaaca agtttgtaca aaaaagcagg ctggcgccgg
     8821 aaccgaagtt cctattccga agttcctatt ctctagaaag tataggaact tcgaaccctt
     8881 tcccacacca ccctccacac ttgccccaaa cactgccaac tatgtaggag gaaggggttg
     8941 ggactaacag aagaacccgt tgtggggaag ctgttgggag ggtcacttta tgttcttgcc
     9001 caaggtcagt tgggtggcct gcttctgatg aggtggtccc aaggtctggg gtagaaggtg
     9061 agagggacag gccaccaagg tcagcccccc ccccctatcc cataggagcc aggtccctct
     9121 cctggacagg aagactgaag gggagatgcc agagactcag tgaagcctgg ggtaccctat
     9181 tggagtcctt caaggaaaca aacttggcct caccaggcct cagccttggc tcctcctggg
     9241 aactctactg cccttgggat ccccttgtag ttgtgggtta cataggaagg gggacgggat
     9301 tccccttgac tggctagcct actcttttct tcagtcttct ccatctcctc tcacctgtct
     9361 ctcgaccctt tccctaggat agacttggaa aaagataagg ggagaaaaca aatgcaaacg
     9421 aggccagaaa gattttggct gggcattcct tccgctagct tttattggga tcccctagtt
     9481 tgtgataggc cttttagcta catctgccaa tccatctcat tttcacacac acacacacca
     9541 ctttccttct ggtcagtggg cacatgtcca gcctcaagtt tatatcacca cccccaatgc
     9601 ccaacacttg tatggccttg ggcgggtcat cccccccccc acccccagta tctgcaacct
     9661 caagctagct tgggtgcgtt ggttgtggat aagtagctag actccagcaa ccagtaacct
     9721 ctgccctttc tcctccatga caaccaggtc ccaggtcccg aaaaccaaag aagaagaacc
     9781 ctaacaaaga ggacaagcgg cctcgcacag ccttcactgc tgagcagctc cagaggctca
     9841 aggctgagtt tcagaccaac aggtacctga cagagcagcg gcgccagagt ctggcacagg
     9901 agctcggtac ccggaagatc tggactctag agaattccgc ccctctccct cccccccccc
     9961 taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt
    10021 ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt
    10081 gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt
    10141 cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct
    10201 ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt
    10261 ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt
    10321 ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa
    10381 ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta
    10441 gtcgaggtta aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa
    10501 acacgatgat aagcttgcca caaccatgga agatcccgtc gttttacaac gtcgtgactg
    10561 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
    10621 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
    10681 cgaatggcgc tttgcctggt ttccggcacc agaagcggtg ccggaaagct ggctggagtg
    10741 cgatcttcct gaggccgata ctgtcgtcgt cccctcaaac tggcagatgc acggttacga
    10801 tgcgcccatc tacaccaacg tgacctatcc cattacggtc aatccgccgt ttgttcccac
    10861 ggagaatccg acgggttgtt actcgctcac atttaatgtt gatgaaagct ggctacagga
    10921 aggccagacg cgaattattt ttgatggcgt taactcggcg tttcatctgt ggtgcaacgg
    10981 gcgctgggtc ggttacggcc aggacagtcg tttgccgtct gaatttgacc tgagcgcatt
    11041 tttacgcgcc ggagaaaacc gcctcgcggt gatggtgctg cgctggagtg acggcagtta
    11101 tctggaagat caggatatgt ggcggatgag cggcattttc cgtgacgtct cgttgctgca
    11161 taaaccgact acacaaatca gcgatttcca tgttgccact cgctttaatg atgatttcag
    11221 ccgcgctgta ctggaggctg aagttcagat gtgcggcgag ttgcgtgact acctacgggt
    11281 aacagtttct ttatggcagg gtgaaacgca ggtcgccagc ggcaccgcgc ctttcggcgg
    11341 tgaaattatc gatgagcgtg gtggttatgc cgatcgcgtc acactacgtc tgaacgtcga
    11401 aaacccgaaa ctgtggagcg ccgaaatccc gaatctctat cgtgcggtgg ttgaactgca
    11461 caccgccgac ggcacgctga ttgaagcaga agcctgcgat gtcggtttcc gcgaggtgcg
    11521 gattgaaaat ggtctgctgc tgctgaacgg caagccgttg ctgattcgag gcgttaaccg
    11581 tcacgagcat catcctctgc atggtcaggt catggatgag cagacgatgg tgcaggatat
    11641 cctgctgatg aagcagaaca actttaacgc cgtgcgctgt tcgcattatc cgaaccatcc
    11701 gctgtggtac acgctgtgcg accgctacgg cctgtatgtg gtggatgaag ccaatattga
    11761 aacccacggc atggtgccaa tgaatcgtct gaccgatgat ccgcgctggc taccggcgat
    11821 gagcgaacgc gtaacgcgaa tggtgcagcg cgatcgtaat cacccgagtg tgatcatctg
    11881 gtcgctgggg aatgaatcag gccacggcgc taatcacgac gcgctgtatc gctggatcaa
    11941 atctgtcgat ccttcccgcc cggtgcagta tgaaggcggc ggagccgaca ccacggccac
    12001 cgatattatt tgcccgatgt acgcgcgcgt ggatgaagac cagcccttcc cggctgtgcc
    12061 gaaatggtcc atcaaaaaat ggctttcgct acctggagag acgcgcccgc tgatcctttg
    12121 cgaatacgcc cacgcgatgg gtaacagtct tggcggtttc gctaaatact ggcaggcgtt
    12181 tcgtcagtat ccccgtttac agggcggctt cgtctgggac tgggtggatc agtcgctgat
    12241 taaatatgat gaaaacggca acccgtggtc ggcttacggc ggtgattttg gcgatacgcc
    12301 gaacgatcgc cagttctgta tgaacggtct ggtctttgcc gaccgcacgc cgcatccagc
    12361 gctgacggaa gcaaaacacc agcagcagtt tttccagttc cgtttatccg ggcaaaccat
    12421 cgaagtgacc agcgaatacc tgttccgtca tagcgataac gagctcctgc actggatggt
    12481 ggcgctggat ggtaagccgc tggcaagcgg tgaagtgcct ctggatgtcg ctccacaagg
    12541 taaacagttg attgaactgc ctgaactacc gcagccggag agcgccgggc aactctggct
    12601 cacagtacgc gtagtgcaac cgaacgcgac cgcatggtca gaagccgggc acatcagcgc
    12661 ctggcagcag tggcgtctgg cggaaaacct cagtgtgacg ctccccgccg cgtcccacgc
    12721 catcccgcat ctgaccacca gcgaaatgga tttttgcatc gagctgggta ataagcgttg
    12781 gcaatttaac cgccagtcag gctttctttc acagatgtgg attggcgata aaaaacaact
    12841 gctgacgccg ctgcgcgatc agttcacccg tgcaccgctg gataacgaca ttggcgtaag
    12901 tgaagcgacc cgcattgacc ctaacgcctg ggtcgaacgc tggaaggcgg cgggccatta
    12961 ccaggccgaa gcagcgttgt tgcagtgcac ggcagataca cttgctgatg cggtgctgat
    13021 tacgaccgct cacgcgtggc agcatcaggg gaaaacctta tttatcagcc ggaaaaccta
    13081 ccggattgat ggtagtggtc aaatggcgat taccgttgat gttgaagtgg cgagcgatac
    13141 accgcatccg gcgcggattg gcctgaactg ccagctggcg caggtagcag agcgggtaaa
    13201 ctggctcgga ttagggccgc aagaaaacta tcccgaccgc cttactgccg cctgttttga
    13261 ccgctgggat ctgccattgt cagacatgta taccccgtac gtcttcccga gcgaaaacgg
    13321 tctgcgctgc gggacgcgcg aattgaatta tggcccacac cagtggcgcg gcgacttcca
    13381 gttcaacatc agccgctaca gtcaacagca actgatggaa accagccatc gccatctgct
    13441 gcacgcggaa gaaggcacat ggctgaatat cgacggtttc catatgggga ttggtggcga
    13501 cgactcctgg agcccgtcag tatcggcgga attccagctg agcgccggtc gctaccatta
    13561 ccagttggtc tggtgtcaaa aataataata accgggcagg ggggatctaa gctctagata
    13621 agtaatgatc ataatcagcc atatcacatc tgtagaggtt ttacttgctt taaaaaacct
    13681 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
    13741 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
    13801 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
    13861 ctggatccgg aataacttcg tatagcatac attatacgaa gttatgttta aacggcgcgc
    13921 cccggaattc gccttctgca ggagcgtaca gaacccaggg ccctggcacc cgtgcagacc
    13981 ctggcccacc ccacctgggc gctcagtgcc caagagatgt ccacacctag gatgtcccgc
    14041 ggtgggtggg gggcccgaga gacgggcagg ccgggggcag gcctggccat gcggggccga
    14101 accgggcact gcccagcgtg ggcgcggggg ccacggcgcg cgcccccagc ccccgggccc
    14161 agcaccccaa ggcggccaac gccaaaactc tccctcctcc tcttcctcaa tctcgctctc
    14221 gctctttttt tttttcgcaa aaggagggga gagggggtaa aaaaatgctg cactgtgcgg
    14281 cgaagccggt gagtgagcgg cgcggggcca atcagcgtgc gccgttccga aagttgcctt
    14341 ttatggctcg agcggccgcg gcggcgccct ataaaaccca gcggcgcgac gcgccaccac
    14401 cgccgagacc gcgtccgccc cgcgagcaca gagcctcgcc tttgccgatc ctctagagtc
    14461 gagatccgcc gccaccatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
    14521 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
    14581 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
    14641 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt
    14701 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
    14761 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
    14821 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
    14881 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
    14941 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
    15001 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
    15061 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
    15121 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
    15181 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
    15241 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
    15301 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
    15361 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
    15421 atgctggagt tcttcgccca ccccccggat ctaagctcta gataagtaat gatcataatc
    15481 agccatatca catctgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
    15541 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
    15601 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
    15661 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat ccgggggtac
    15721 cgcgtcgaga agttcctatt ccgaagttcc tattctctag aaagtatagg aacttcgtcg
    15781 agataacttc gtatagcata cattatacga agttatgtcg agatatctag acccagcttt
    15841 cttgtacaaa gtggttgata tctctatagt cgcagtaggc ggactaaacc attgtcaaca
    15901 aaggacaaag atcctccaaa ggcaaggagt acacctgtga cttagggacc tctggtcatt
    15961 tgcattgttt atcacttagc atctccagag gaaccattta ataaacgtaa cagtttttat
    16021 ttaacattta aataaaataa aaatatttaa tattaaaata aaatattgaa cctttgccca
    16081 aggtgcttta cattggaatc taccagaata taccagattc tacacacatc ccaagttcaa
    16141 gtgaccctca caaagctctt gtgccatgtt atatccattc ctattctatt tttagctcat
    16201 gatactgggg ctccaagagg aggctccggg ctcctttctt ctgacataca cagacctagg
    16261 cccacccctg tctgacagcc ttcttcatcc tctccagcca aacttgacag caactttggc
    16321 tgccggtccg tcaaagtgaa ggactaccac ttaggatacg gcatgacaat caaggtaccg
    16381 atgatccata acatggccat gcattacttg ttccgagtcg aggatctgtc tagcacggtg
    16441 ctgatgctta ctctgctcac aggcaacttc gcaacctact tcatcatacc cgatccaggg
    16501 aagatgcaga aggtggaaca gagcctaacc tacccacact tccgccggat gagacgacaa
    16561 ttattgacaa ggtgatcccc agtcccagga actacccaga ggcatccaac ctcttattca
    16621 ttaaataaca aaggccctag aagactggcc agaagctggg gccacagcca ctttgagctt
    16681 ccaagcagac tctgtgttcc tttcagtaat gcaggccagg ttcactgaca aaagcagtgc
    16741 tcaatataaa gaggtaaaca tgtgatccat tggagatggc gcaacgcaat taatgataac
    16801 ttcgtatagc atacattata cgaagttatg gtctgagctc gccatcagtt cagccacagt
    16861 tgtagtgtat attatggctg agctaagaag atgaagatga ctctcaggac ctggccacat
    16921 taaaggttag aggtcaaagt ggacagtcca cgagggtgtt ttggcgacag gggaagaagc
    16981 tatatggaaa gcctcaggaa tagagaaccc agggcatgac tacaagagac tcaatttatc
    17041 tgatgctctt gcatgggttt attgggtccc tagagccatg ctccacaaat cagtgcttct
    17101 ggtgctctgt aaacaagcag ggccttgcta ccagttgcat gacacaggct acatgaaaca
    17161 cagggacact cgggcctgaa ctatagaccc tcaccagtga gcagttgtac tcggctgctc
    17221 tgactcccac tctctcgaga gtcaggtctt gggggaatgt ctggctgagt aagaggggtg
    17281 gccaaacact gcaggaacat gattgcatgt gaacaccctg agtctgcctg aagggtttct
    17341 ctgaggagat ggcattttag ggacaactag gacaaaggta ttggttcttc cagactaaag
    17401 aaaagcttcc ttttctacac accaggttgg ttgatttaga gataccagaa ctctcattgt
    17461 ctgagaccca tgacttggag tcaatgatga gtctactagg aatcacctat gttttcaaca
    17521 gtggcaccaa tagctctgac atgaatgaca cacttcagaa gtccttcaag gtaaggtcac
    17581 ctcacagtga cagtcctgtg tctgttggag tcaagtaggg tagaggtgca gctcagatgt
    17641 gaggctaagg gtagctagcg gaaaggatac agaccaaggc acggctgagc tcctccagag
    17701 ggacctttgg gatttatcag gaggatgggg tcagaggtca aaactacaac agggagagtc
    17761 ttgtgcttca gataataaaa gtagctctcc tgaatggcct tcctgagcct cagtcttaca
    17821 gttgcccagg ccagttgaga ccttggggtt caatttgcct atatttgtac agagaggatt
    17881 gggcccactg acctaggata ttatcctaga tttttttttc acctgtggaa tttagagcaa
    17941 ctcaagagat cggagtgtta ttgagttttc caaacatacc actggttcgg acaattaaag
    18001 atagatcagg atgagcaccc ttggtgtctg gacactcgag gtcgtcttca gaaaccctat
    18061 ccccaaagag ttcacagcta tacactggaa aggggaccct tgtgccaagg acgtgaaata
    18121 caaatgcccc ctcaacatac tcactgtaac gctgtgaaat gtatctaaga ctatgagaga
    18181 ttgagtcagg aaaggaacat gaaagggaat cggccaaagc aggcaaagaa taggagggtg
    18241 gggtgggaag ggctttgggt caccaccatg gatcaggctg agggctgggc agtaccataa
    18301 aggatggaca taagccattg gagggtcttc ctttgtaagt tccacatatg atcataccct
    18361 aggccagagc aggtgttgcc tgcttctgac aagctcctgc ttctcttcct acaggtggta
    18421 agtaaggctg tgctgaccat cgatgaaaag gggtcaaagc ctagtacaaa ctcatgcttt
    18481 aaaaagctcg gatcgactga tatgggccgg atgcagctca acagaccttt cctcatcttc
    18541 atccaggatc acacaaatga tgtccccctc tttttaggca gagtggtaaa tccccagaac
    18601 taagtgctct caggaatcag cctctctcct gaaagccatg tccccttctg ggtaacatta
    18661 aacatggggc cgtttcaccc atgcctgagt gcaaacagca aatccctctc cttgtggtgt
    18721 ctgtgtcttc ctctgtcccc tgaggtatgt ggggtctcca ggtcacagtg catcacactg
    18781 tctcctgcat ctgtgttgag gaggtcccca tgatgtccca gctccagcac tgaaggcaga
    18841 gggccatgtc cccttctggt tgtactgtgt catctcactg ggtctggcct ctccatctca
    18901 ccacaatttt cctgagaatg gctagaggaa atagttggta gaactcttcc tccacaggac
    18961 tggagcagga ccccagcctg ttgctgtcat tcgttccctg ctagaaggga ctcacaaatc
    19021 ctccaatgat cagagaggtc tctgggtgat tcccctcggg cttctcctgc caatcaggcc
    19081 actagacaca aagccacttt gtcctggctg accgtgaagc catgctgtat gagtggctct
    19141 gaaaagccat aaggataggt acctacatga gaaggacacc tttcctttcg ctagggtccc
    19201 cctgagatct tcctgaagtc atatacaact gcacctaccc catctccagg ccctcaacat
    19261 agtattccag ggataaatat aaatccagtg cctcatagta gatctcagga ttcatcattg
    19321 tcatcggggc caggcagtct accccatcct cctcaaccct tgttatgccc agatcttggg
    19381 aaccccagaa gaactctagg aattgctagt ccaatgtaat ataactgaag agcctttatt
    19441 cgagtcatac aaggatcaaa aacctttccc actgcacagg ttaggaacgg gatgctcgct
    19501 gttccagttc agaggcttgg agtttttata cgtgcatatg cataagcagg gatccttggg
    19561 ttacatgaca ggggaggtga caagaggaag tcattctttc aaacctgatg tcgtttgttc
    19621 agacagtgca gaattgtgcg actgggaggg ggctcttctg acctgggaag taactttatg
    19681 gtgctatcag gaactagtgt gtttttacaa actggccagg gctgtctggg gacacttaat
    19741 tgcccttctc tcatggccca agggtggggc acttatcttt tcaaggatgt ttgatcctta
    19801 tttctctcaa ggacaggagc catctgcacc agagaaatgt tatcaaggag agtgccagcc
    19861 ttggtgacac agaggctggg atatgttgcc tttgtctgta cccaaagcca ccatctgctg
    19921 ctcccctggt ggtgggagag ggaggggagt gttgaaaggg tcattaggga gaagagagaa
    19981 ctaccaggag aaggggaatt gcaatattgt ggagccaggc tgggccctct ctgtttgccc
    20041 tttctgaaag gctggaggca tctatgctgg gagaaggggg aggggattgg agtggtggga
    20101 gagtgcagag gcaggtttcc tgcatctctt caccctcagc cttgctaact gcctgcagca
    20161 gacagaatcc ccttcccagg aaatgcacct actgctcaga acacttgtgt actgtgggag
    20221 tgctgcctgc ttcatctgca gatcataccc caccccaacc ctaactccct gaataaatgt
    20281 ggacagaaag cactgacttg aatcataaga ttgttacatt ttagataaaa aagaatgtca
    20341 tagttgtgtg atttccaagg actcagtaat caacacacac caagtatttc tttaaaagcc
    20401 acacttctcc ttatatcaat gacctagtcg ctccgacctt ccccagctcc accaaagttg
    20461 agaccatcct aacggctgca ctgactctgg actgaagggt gtcaggacct gggttctagt
    20521 tgagactgat acacaccaga tgtgtgactt ctgtgaaatc atcacactca tgacctctgt
    20581 attcttcttg gggatgataa agtctatttt gatttggtat gttttttttt ccatctcagt
    20641 agcccatgtg ccattgtgag ggatggtgtc cttctgttga gactattcac tgggagtcac
    20701 acacacactc acacacacac tcacacacac acacacacac acacacacac acacacacac
    20761 acacacacac acatacacac acactcacac acacacacac aaagaggtac tggagatcat
    20821 tctttccctg actgtccata gtagcacttc acagatctga tgagaaagga ggcaccattc
    20881 tctttgtgag taaaccgaag ccaagcaagc caaattgagc tgtcacttgc cagtggttaa
    20941 ggggctgctc accgggacac aaggagaagc tggtggttag ggttctatgg tgggctaaag
    21001 tttggccctt gttcaaactt tatgtcattc tgagcattgt ttcaaaatgt ctcatcctgg
    21061 agcactctgg ccttgaagat cttaggacac catgcttccc tgatcctcgc agctgtcact
    21121 gggagccagg aattaatcat tgtcctcaac atccgacatc cgacaggagt acagagcaag
    21181 gtcactgggt ggcagccggc attatttgaa cagcttttcc ttctgttcta gaactagggc
    21241 tctggggaga aagtcggtgt gttccctgtt gtgtttgtgt gacctccgat gagagaggtc
    21301 tccacattgg ggtgtatgtg cacatgtgtt tctatagcat atctgtatgt gctagtgtgt
    21361 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gcgcgcgcgc acgcgcatgc
    21421 ttgcacatgt gcaagtgttt ctgtcattgt cagtctttgt gcatacgtat atctgtgtca
    21481 gagaacatgc gtctgtccag ggggtgtgat acctggtgca gatggatatg gcacaatgaa
    21541 tggcctttac tctctccacc ctcgaggcaa aagtcccctt ccctggcatg gacaccacca
    21601 gtcttttcct caggcacacc aaggacccct tttgtaacat tagacacttg agttatctct
    21661 tctctg
//