LOCUS       KO-first_condition_ready_28383_MGI:1354961        21831 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Synj1 targeting project(s): 28383
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_Bact_P
COMMENT     design_id: 46942
COMMENT     backbone: L3L4_pD223_DTA_T_spec
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            7149..7235
                     /db_xref="ENSEMBL:ENSMUSE00001237810"
                     /rank="3"
                     /note="ENSMUSE00001237810"
     misc_feature    4275..8938
                     /note="5 arm"
     misc_feature    8966..8990
                     /note="B1 Gateway"
     misc_feature    9003..9050
                     /note="Frt"
     intron          9051..9925
                     /note="mouse En2 intron"
     misc_feature    9923..9925
                     /note="Splice Acceptor"
     exon            9926..10098
                     /note="mouse En2 exon"
     misc_feature    10110..10703
                     /note="ECMV IRES"
     gene            10704..13764
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    13803..14046
                     /note="SV40 polyadenylation site"
     misc_feature    14050..14083
                     /note="loxP"
     promoter        14110..14629
                     /note="human beta actin promoter"
     gene            14655..15449
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    15649..15890
                     /note="SV40 polyadenylation site"
     misc_feature    15907..15954
                     /note="Frt"
     misc_feature    15961..15994
                     /note="loxP"
     misc_feature    9940..10017
                     /note="FCHK std_gpr"
     misc_feature    16010..16030
                     /note="B2 Gateway"
     misc_feature    8939..16060
                     /note="Synthetic Cassette"
     exon            16564..16831
                     /db_xref="ENSEMBL:ENSMUSE00001229090"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 1 ENSMUSE00001229090"
     misc_feature    16061..17040
                     /note="Critical Region"
     misc_feature    17064..17097
                     /note="Downstream LoxP"
     misc_feature    17041..17120
                     /note="synthetic loxP region"
     exon            20829..20912
                     /db_xref="ENSEMBL:ENSMUSE00001227494"
                     /rank="6"
                     /note="ENSMUSE00001227494"
     exon            19885..20110
                     /db_xref="ENSEMBL:ENSMUSE00001218733"
                     /rank="5"
                     /note="ENSMUSE00001218733"
     misc_feature    17121..21831
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggattgtgg aacttctagt ctatacgcat attcagggag taataacatt
     4321 cattattatt tcctttgttg cttatactct acatttttca gtaccattga acatgtagca
     4381 atggaaccta ccctaagttc aagagcagga agtaggtagc tcttccaggg tcccacactg
     4441 tacccctggg accaggttca cctgcctctg catctccaat gctgggatta aagtattagt
     4501 cactgtgccc agaaaagagg ttccctctaa tgctggctcc ttagacttag acaattagta
     4561 actgacttaa gccaatttcc tcaggtcaaa tggaaggtgt agctttgtcc ttagggtact
     4621 gtgaaaatta aatagtgcac ataaacacct ggccttgtgt tagtgccata atcatcgcag
     4681 agatgatgaa ctaatactgt agctgtaaga ccatttctaa attgctggta atagaaactc
     4741 atggcagtaa aaatgtaacc tcgggctggt gagatggctc agtgggtaag agcacctgac
     4801 tgttcttccg aaggtccaga gttcaaatcc cagcaaccac atggtggctt acaaccatcc
     4861 gtaacaagat ctgactccct cttctggtgt gtctgaagac agctacagtg tacttacata
     4921 taataataaa taaatcttaa aaaaaaagaa agaaaaaaaa acaataataa aaaaaaaatg
     4981 taacctcatc tgctttagtc agagagagag agaactcaga agataactct gaaatacaaa
     5041 taattaagtg gcttggtgta tgcttacatg tttaagctag atgacggtgc aatagaagga
     5101 tggtcactgg gcctgagaga cagtgtttat ttgttgtgca attgagaaga aaatagttat
     5161 gaggagagaa tatttatttc atgataattt aggttacaga gactattcat atggggagag
     5221 aattacttaa tattaagtta cattttcttt ttaactcttg ctggctgtgg tcattgaacc
     5281 taatggtgat tttttgctaa ctgctaattt aacctaattg ctgctgacac taatacatca
     5341 gtaattcctt cattccaatg acagcaataa tattttaaat tcacacagca tgtagcctga
     5401 gggactccgt agagcagtct gacctggaaa ccgcattttc tgccatgaaa aattgagacc
     5461 cactcccagc cccacccatg gtacgctgaa tgaggaagtg cagcacttaa gaaaggcagc
     5521 tgtagtttcc tttagaaaac aattcagttt ttgttgtaaa ttttaaattt gaattgacca
     5581 taaacacata gaacaatata atgctaggac ggctagatat gtgaaagaag acacgattcc
     5641 cctagcatgc taaaagtctt ccccactcta gtttcatact ggttacagaa aggcccacac
     5701 ttaataatga acatattaga gaatgtgatg gtgttagttg agtcatttag ctttagcctg
     5761 tgctgccagc taactgacca taagtccccg gctggccaaa gtgagccgcg gaggagaagc
     5821 ttgagtagac agggtggaga gaggcagatg gggaagtgag atactatgtg gaactctggg
     5881 gaagagcttt aagataagag tctggaatcc aaggaattgg caaggaatct tggataagat
     5941 gtagattgag aagagcctgt gtctgcagag aagatagaca ggaaatggga ggtcagagca
     6001 atctgcttac ttgctttcct agagcagcat acaggagccc tgccaagggt tctgtggttg
     6061 tggtgtggac agaggggagg gagaaggcta agtgtgatga ggacagggaa tcctgtgacc
     6121 tagcaagcct cctcgagctg gggagaggtg ggacagggaa cggagggcag tggctaagtt
     6181 tgaatttcac acttcagaat gtgctatttg aaattctttg aaataccaga tgtggtttat
     6241 gttccttggt tctttgttgt atcttaaatg tggatctgct aagtgctagg aaggttctgc
     6301 ctttaaacat ccgggatttt tgttgttgtt gtcttttatt ttattttatt tttttatcag
     6361 tagactaacc atagtagcct gctctaggga aattattgcc tggcttgagc tcattagcaa
     6421 gtttgcctca gcttagagca aaggaagtga gaggaaggtc agtaaagact gccccactgc
     6481 catgctaaac cgtgtggtca agcagtggct tgcagcattt accagtttat tcagcttctg
     6541 tgtccaggat aaacgagtga gtaaaacagg ctatcaacaa gaagacagac tccttagatg
     6601 ggcagaattc tgtaactcca gcaagaaagt tcttctacta gttggacaga atacctaata
     6661 taatacactg gtgtttggag aggtcaccta tatcaaacat ggagatactg agctggtgcc
     6721 cacagagact tcatccctat gttccagcat ccttgataca gaaaggtatt ctgcacatta
     6781 tcaagggtaa tgtaaatacc aagccagcta caaaaccttt attcacaaca gtgtccagcc
     6841 tgtaagatat gctaaggtaa tggtggcaca gggcttgcag aagtaaccca ccaacaactg
     6901 atttgactcg aggctcactt cacgaggtga acccagatct gacacagctt gagtggtcaa
     6961 gaaccagaga ctagataacc cagtgacata gggggaaacc aagtcatgct ggtctaaaga
     7021 cagagagaga aaaaagagaa aatagataga tagataaatg gggaaaacga tcccattttg
     7081 aagctttgtt tcaagtctgg cattacaact aaatagattc taatgttttc aattattatt
     7141 tttaacagca tctgcagaaa aggaggctat taaaggcaca tatgccaaag tactggatgc
     7201 atacggactt ttgggagttt tacgattaaa tcttggtaag tctgttattc cgtgtggtta
     7261 atctactttt tcatttctgt ttttatagtt ctgaagatga gagagactac ctgtatatca
     7321 tttcaaaccc agtatttgag ccttaactag tatatacctt ttaaacattc attaggataa
     7381 tctttttaaa tatttttatt aactgagaat ttcatgtaat gtatttgatt atattcaacc
     7441 ccccccaact cctcccagat ctatgcccac atcctcaccc aacatactaa ctctttctct
     7501 ctccctccct tcctctctct caatctctct ttgtccctcc ctcccccact cctctctcaa
     7561 acctatctat gcattggtca agcactcttg gctgtgggat ccattcctga tgtgtgatcg
     7621 gtacatcaga gatcacacca ttaaaggaag cagactgtct ttgctggcag ctgtctggct
     7681 cagtggtcaa gagcacttgg tcctcttgca gaggacccag gtttgcttcc caggaccgat
     7741 atagtagctc acaaccatat ataacttcag ggttacatgt agtgaacata gatttatgca
     7801 gagaaaatgc gcctacacac acaataaata tgcttcttcc tagatagagt tttaggatcc
     7861 aaggagcgtg ttctattgaa atacatcagc agacaatgaa agcagtatct ttcttctcag
     7921 tgtattaggg aataggtgtc tgtgtggtaa acacactgat tcccctagtg tattgtggga
     7981 aaggcatctg ttttagattc agaaatgtct gctatgtaat gttctttttt aaacttaaaa
     8041 tcatttattc ttatgtgtgt gaatgtttga tgcatgtcat gtatgtctgt aaaagatgtg
     8101 cacatagccc actgaattaa gaagagggtg tcagatcccc taggactaga gttcagacag
     8161 ttgaaagcca ccatgtgggt gctggacatt gaacttgggt cccctgaaag agcaggactc
     8221 ttaatcactg agcaatctct ccagccctac ttaatgttac catggtcttc agggtgactt
     8281 ttatgattag aagtttgtgt ataaaggcat gacatgaagt gtggttcaga gactgggcct
     8341 taaccatgtt tttaatggtc tgtgtatgac agtaagctcg cgttatcatg catatataac
     8401 atgtggtttt cttccttgat gaagtctaac tatgaaaatt gtttttaaaa gtaagtaaac
     8461 aaaagtatgg atttagttat acagtaggct ttcattacga ttcaagcact tttcttattg
     8521 tgtgctgcca agagttgatg gagtctcttt gagcagcact ggctatcaag tagaggttta
     8581 ccctcgttag taatctgtca tatgcaaaag ggcgtgctgg atcgtttact gttcttgtct
     8641 tgagttccat gaaataaatg ttttaatccc atttcatagg gggttatgtt acaggttgat
     8701 catgctagca tactcagttt agtctggatt ataagagcca aggttcagag ctatgtctgc
     8761 ctgtgccagt gcttatgttg ctaactctta atttcataag atttttgttg ccattacatg
     8821 aaaaatttga aaatcctggt gttcaggagt taggtccaca gaatgaaccc caatatcatc
     8881 cctttgccac aaaaaggata gaaaacaaaa ccacacaaag cctgaaagtt ttcatgaaaa
     8941 ggcgcataac gataccacga tatcaacaag tttgtacaaa aaagcaggct ggcgccggaa
     9001 ccgaagttcc tattccgaag ttcctattct ctagaaagta taggaacttc gaaccctttc
     9061 ccacaccacc ctccacactt gccccaaaca ctgccaacta tgtaggagga aggggttggg
     9121 actaacagaa gaacccgttg tggggaagct gttgggaggg tcactttatg ttcttgccca
     9181 aggtcagttg ggtggcctgc ttctgatgag gtggtcccaa ggtctggggt agaaggtgag
     9241 agggacaggc caccaaggtc agcccccccc ccctatccca taggagccag gtccctctcc
     9301 tggacaggaa gactgaaggg gagatgccag agactcagtg aagcctgggg taccctattg
     9361 gagtccttca aggaaacaaa cttggcctca ccaggcctca gccttggctc ctcctgggaa
     9421 ctctactgcc cttgggatcc ccttgtagtt gtgggttaca taggaagggg gacgggattc
     9481 cccttgactg gctagcctac tcttttcttc agtcttctcc atctcctctc acctgtctct
     9541 cgaccctttc cctaggatag acttggaaaa agataagggg agaaaacaaa tgcaaacgag
     9601 gccagaaaga ttttggctgg gcattccttc cgctagcttt tattgggatc ccctagtttg
     9661 tgataggcct tttagctaca tctgccaatc catctcattt tcacacacac acacaccact
     9721 ttccttctgg tcagtgggca catgtccagc ctcaagttta tatcaccacc cccaatgccc
     9781 aacacttgta tggccttggg cgggtcatcc ccccccccac ccccagtatc tgcaacctca
     9841 agctagcttg ggtgcgttgg ttgtggataa gtagctagac tccagcaacc agtaacctct
     9901 gccctttctc ctccatgaca accaggtccc aggtcccgaa aaccaaagaa gaagaaccct
     9961 aacaaagagg acaagcggcc tcgcacagcc ttcactgctg agcagctcca gaggctcaag
    10021 gctgagtttc agaccaacag gtacctgaca gagcagcggc gccagagtct ggcacaggag
    10081 ctcggtaccc ggaagatctg gactctagag aattccgccc ctctccctcc ccccccccta
    10141 acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt
    10201 ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga
    10261 cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg
    10321 tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt
    10381 gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat
    10441 aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg
    10501 aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg
    10561 taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt
    10621 cgaggttaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac
    10681 acgatgataa gcttgccaca accatggaag atcccgtcgt tttacaacgt cgtgactggg
    10741 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
    10801 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
    10861 aatggcgctt tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg ctggagtgcg
    10921 atcttcctga ggccgatact gtcgtcgtcc cctcaaactg gcagatgcac ggttacgatg
    10981 cgcccatcta caccaacgtg acctatccca ttacggtcaa tccgccgttt gttcccacgg
    11041 agaatccgac gggttgttac tcgctcacat ttaatgttga tgaaagctgg ctacaggaag
    11101 gccagacgcg aattattttt gatggcgtta actcggcgtt tcatctgtgg tgcaacgggc
    11161 gctgggtcgg ttacggccag gacagtcgtt tgccgtctga atttgacctg agcgcatttt
    11221 tacgcgccgg agaaaaccgc ctcgcggtga tggtgctgcg ctggagtgac ggcagttatc
    11281 tggaagatca ggatatgtgg cggatgagcg gcattttccg tgacgtctcg ttgctgcata
    11341 aaccgactac acaaatcagc gatttccatg ttgccactcg ctttaatgat gatttcagcc
    11401 gcgctgtact ggaggctgaa gttcagatgt gcggcgagtt gcgtgactac ctacgggtaa
    11461 cagtttcttt atggcagggt gaaacgcagg tcgccagcgg caccgcgcct ttcggcggtg
    11521 aaattatcga tgagcgtggt ggttatgccg atcgcgtcac actacgtctg aacgtcgaaa
    11581 acccgaaact gtggagcgcc gaaatcccga atctctatcg tgcggtggtt gaactgcaca
    11641 ccgccgacgg cacgctgatt gaagcagaag cctgcgatgt cggtttccgc gaggtgcgga
    11701 ttgaaaatgg tctgctgctg ctgaacggca agccgttgct gattcgaggc gttaaccgtc
    11761 acgagcatca tcctctgcat ggtcaggtca tggatgagca gacgatggtg caggatatcc
    11821 tgctgatgaa gcagaacaac tttaacgccg tgcgctgttc gcattatccg aaccatccgc
    11881 tgtggtacac gctgtgcgac cgctacggcc tgtatgtggt ggatgaagcc aatattgaaa
    11941 cccacggcat ggtgccaatg aatcgtctga ccgatgatcc gcgctggcta ccggcgatga
    12001 gcgaacgcgt aacgcgaatg gtgcagcgcg atcgtaatca cccgagtgtg atcatctggt
    12061 cgctggggaa tgaatcaggc cacggcgcta atcacgacgc gctgtatcgc tggatcaaat
    12121 ctgtcgatcc ttcccgcccg gtgcagtatg aaggcggcgg agccgacacc acggccaccg
    12181 atattatttg cccgatgtac gcgcgcgtgg atgaagacca gcccttcccg gctgtgccga
    12241 aatggtccat caaaaaatgg ctttcgctac ctggagagac gcgcccgctg atcctttgcg
    12301 aatacgccca cgcgatgggt aacagtcttg gcggtttcgc taaatactgg caggcgtttc
    12361 gtcagtatcc ccgtttacag ggcggcttcg tctgggactg ggtggatcag tcgctgatta
    12421 aatatgatga aaacggcaac ccgtggtcgg cttacggcgg tgattttggc gatacgccga
    12481 acgatcgcca gttctgtatg aacggtctgg tctttgccga ccgcacgccg catccagcgc
    12541 tgacggaagc aaaacaccag cagcagtttt tccagttccg tttatccggg caaaccatcg
    12601 aagtgaccag cgaatacctg ttccgtcata gcgataacga gctcctgcac tggatggtgg
    12661 cgctggatgg taagccgctg gcaagcggtg aagtgcctct ggatgtcgct ccacaaggta
    12721 aacagttgat tgaactgcct gaactaccgc agccggagag cgccgggcaa ctctggctca
    12781 cagtacgcgt agtgcaaccg aacgcgaccg catggtcaga agccgggcac atcagcgcct
    12841 ggcagcagtg gcgtctggcg gaaaacctca gtgtgacgct ccccgccgcg tcccacgcca
    12901 tcccgcatct gaccaccagc gaaatggatt tttgcatcga gctgggtaat aagcgttggc
    12961 aatttaaccg ccagtcaggc tttctttcac agatgtggat tggcgataaa aaacaactgc
    13021 tgacgccgct gcgcgatcag ttcacccgtg caccgctgga taacgacatt ggcgtaagtg
    13081 aagcgacccg cattgaccct aacgcctggg tcgaacgctg gaaggcggcg ggccattacc
    13141 aggccgaagc agcgttgttg cagtgcacgg cagatacact tgctgatgcg gtgctgatta
    13201 cgaccgctca cgcgtggcag catcagggga aaaccttatt tatcagccgg aaaacctacc
    13261 ggattgatgg tagtggtcaa atggcgatta ccgttgatgt tgaagtggcg agcgatacac
    13321 cgcatccggc gcggattggc ctgaactgcc agctggcgca ggtagcagag cgggtaaact
    13381 ggctcggatt agggccgcaa gaaaactatc ccgaccgcct tactgccgcc tgttttgacc
    13441 gctgggatct gccattgtca gacatgtata ccccgtacgt cttcccgagc gaaaacggtc
    13501 tgcgctgcgg gacgcgcgaa ttgaattatg gcccacacca gtggcgcggc gacttccagt
    13561 tcaacatcag ccgctacagt caacagcaac tgatggaaac cagccatcgc catctgctgc
    13621 acgcggaaga aggcacatgg ctgaatatcg acggtttcca tatggggatt ggtggcgacg
    13681 actcctggag cccgtcagta tcggcggaat tccagctgag cgccggtcgc taccattacc
    13741 agttggtctg gtgtcaaaaa taataataac cgggcagggg ggatctaagc tctagataag
    13801 taatgatcat aatcagccat atcacatctg tagaggtttt acttgcttta aaaaacctcc
    13861 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
    13921 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
    13981 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
    14041 ggatccggaa taacttcgta tagcatacat tatacgaagt tatgtttaaa cggcgcgccc
    14101 cggaattcgc cttctgcagg agcgtacaga acccagggcc ctggcacccg tgcagaccct
    14161 ggcccacccc acctgggcgc tcagtgccca agagatgtcc acacctagga tgtcccgcgg
    14221 tgggtggggg gcccgagaga cgggcaggcc gggggcaggc ctggccatgc ggggccgaac
    14281 cgggcactgc ccagcgtggg cgcgggggcc acggcgcgcg cccccagccc ccgggcccag
    14341 caccccaagg cggccaacgc caaaactctc cctcctcctc ttcctcaatc tcgctctcgc
    14401 tctttttttt tttcgcaaaa ggaggggaga gggggtaaaa aaatgctgca ctgtgcggcg
    14461 aagccggtga gtgagcggcg cggggccaat cagcgtgcgc cgttccgaaa gttgcctttt
    14521 atggctcgag cggccgcggc ggcgccctat aaaacccagc ggcgcgacgc gccaccaccg
    14581 ccgagaccgc gtccgccccg cgagcacaga gcctcgcctt tgccgatcct ctagagtcga
    14641 gatccgccgc caccatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
    14701 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
    14761 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
    14821 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
    14881 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
    14941 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
    15001 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
    15061 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
    15121 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
    15181 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
    15241 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
    15301 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
    15361 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
    15421 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
    15481 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
    15541 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
    15601 gctggagttc ttcgcccacc ccccggatct aagctctaga taagtaatga tcataatcag
    15661 ccatatcaca tctgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
    15721 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
    15781 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
    15841 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcc gggggtaccg
    15901 cgtcgagaag ttcctattcc gaagttccta ttctctagaa agtataggaa cttcgtcgag
    15961 ataacttcgt atagcataca ttatacgaag ttatgtcgag atatctagac ccagctttct
    16021 tgtacaaagt ggttgatatc tctatagtcg cagtaggcgg aaccatttat aagcaactgg
    16081 gacatttact atcttctctg aacttcctgt ccatcagtaa agtagtgaag gcaacttttt
    16141 ctttttcttt cctttttttt ttatttttat aaattttgta accttagatt aggtataaaa
    16201 taactttgta aatgtgacat taatctcatt aatgttttag tgcctcacat actgaatgta
    16261 tttaaacaag atttttcccc ctagaaaaaa aacctgtagt tgtaaattta aacatattga
    16321 taaataatca gaatggctag gcatggtggt ctcacctcta atccctgtgt gtggaaggtt
    16381 taggccagag gattagacgt ttatacagtg tagaaagact agctcaaaaa aagaaacaaa
    16441 aaaattacaa tgtaagattt taaagataga aacacaggaa gaagtcagaa gaaactatgt
    16501 aacttgaagc tataggcttg caaacattga tgttctatcc agtaaaaccg ttggtttttg
    16561 caggtgatac catgctgcac tatctggtcc tagtcactgg atgcatgtct gtggggaaaa
    16621 tccaagaatc tgaagttttc cgagttactt ccactgagtt tatatcattg cgagttgatg
    16681 cttcagatga ggaccgcatt tcggaagtac gaaaagtttt gaactcggga aacttttatt
    16741 ttgcatggtc tgcatctgga gtcagcttag atctgagtct gaatgcacat cgaagcatgc
    16801 aagagcacac aaccgacaat aggtttttct ggtgagtctg tggtatcttt cttcctttgt
    16861 gatgatttac tgtgcggtag ctccgagatc ggggcataat gggctccagg gcggcagttt
    16921 ctggagagaa cagaggctgg aaatggttgc ctactttttc ctgccccgcg cctgcagcag
    16981 agcatggtgg ccccttggtg gagctgccag atgggatgaa gcaattaggt gctcgatctt
    17041 gagatggcgc aacgcaatta atgataactt cgtatagcat acattatacg aagttatggt
    17101 ctgagctcgc catcagttca tcatctctgg catttgtagt gtaggtgtca ctcgttcgtt
    17161 cgctagctta gttccgaggt ctcatctctg acaggaccga cattctaagc agcactttct
    17221 tactttcctc ttaagactgt aaggagctgg tcctctttct tttttggtgc tggaaattga
    17281 acatgctttg tgcatagtag gcggctgagt gctcctctag cccgagtaga tgatcttggt
    17341 atgtgaacag caggctctga agaaatcctg cactaggaca cagtctagca ctgcagtaac
    17401 gaaaacctga gtgggtattc aggctatgtt gcatgacact gctaacgtgc tatgctgtag
    17461 gcaagcacaa atggtgagca gacacatcag actgaaagtt gtgtggcttc attgtagcag
    17521 actgcatttc agtgcttcca gttatgttgc ttctgttgtc tgtggcccgc agcagtgatg
    17581 acttctcttt aatgcagata gaaattactt ttcaccatat taccatacat tgtaaggcta
    17641 aagcccctcg gacagtgctc ttgacccacc tcggtcttca gattccaggc atttgcttct
    17701 gaggccatcc cccgtctccc gctcagcatc atgcgtggtt cttggcttgt acattcaggt
    17761 ctgacacatt tgccccggct ctgagagggc tccaagttaa ggacagcttg aggagagaag
    17821 ccaaacgtca tagcttatgt ggaagaggct tgactcaaag gagagagagt ggagcagagg
    17881 ggacagaata aacaaaggct gtagagcact gcctgtcagg gactcgggct tggcaaacta
    17941 gaagaagaca gatgtaatgt gcttttgtca agcatgcaca gagaggcttt ccagagtgaa
    18001 acctttgcag atcctatgtt ttttctagtg ggaaagaaac cctgaagccc ttgaagtaaa
    18061 tagtcatagc ccagctgggg cagctggcca tgccctctgt cctcactcct atctccatct
    18121 cagatggaca gcacgggaga gatccaagca ggtgcgattg catttacagt ttaattagta
    18181 gactaattct gttttctttt ctgtttgtta attaaaggtg gggatcagga catatttaga
    18241 tcttagcccg agggaggttg ttgtccagtg ggtcaataga ttgaacttgt gttttgctaa
    18301 agattgaaat acttcagttt aattttgcct agaaaaaatg ggaagtttta gacttcacat
    18361 cattgtagct ttgtcttttt ttttcctttg aaagatatac agtaagcttt gcagctagat
    18421 tgccaagtac ctctctcaag aactgtatgg ttatatttca ttggagagtt taagctgcta
    18481 ctactcaaga cttggcatac atgtaaatag aataatacat caatacattg acaggaaggg
    18541 gaaatgtaaa ttgacaacag agcagagaaa agctacctgc tcccaatgcc tcagatctaa
    18601 gctccactta agtgaggaca accgcatcac ttgtctgcac catggcatag cccttgcttg
    18661 atctttggac atattgggtc ataattggtt gaggtttgcc tcagatcagt gtaaataata
    18721 aatagtaaag tatgagagcg taagtcacgg tattctaaat aagtaatggc tgtcggacat
    18781 cgcatgatgc tggtagtggt cgcatctctc agttgcattc cagtttgctt tgtaaatagc
    18841 aggaaggttt aggatggtag ctggtctata acaagtgtgc atggcctcca cctagtgtta
    18901 actgaaaggc agctgtgttc cttgttactg tgttcaagag ggcgcgatca catttatttt
    18961 tctctctttg taagatacca gggtattgta aaacacttaa agtccattta agatgggtca
    19021 acatcttaaa actctgagct tacaataata tagaaaagta acttttgaac tggcagagtc
    19081 gtttgctgaa aggtgtcttt ttttagtggg ttccttagaa tactccagag tcttccatat
    19141 gtgttctgtg gaagactgag gttccaatag agacagactt ctgaagagta ttgtgcagca
    19201 ggagaagact aacttgctag ggctcactcc tccatccatg ctttagtcat tgcgacacta
    19261 aatattttta tcttttggtt ttgaccattg ggctgcgtct ttgcttgaat aaaacatttt
    19321 tcccttcaca tttgtacact aggccttatc tgatacctgc agtactccag tgtttagatg
    19381 ggtctatggg tgtgctcaga gataaagcat ttactatcca atgagtgagg tcctagatgc
    19441 cgtgaccaaa actgaaacaa aaacagtttt tggagacata gaaaacactg tgccttacat
    19501 tgtgtgatat gacagctact aactatgctg gttatttaaa cttcattaaa atatagcctt
    19561 atatataagt gtccttatat atcttatata taagtctctt taaagtaggg tgtgtttgca
    19621 aaactccatt tgacaaggta agttctgtgc ttgttgtagt ttatacacca gaaatgtttt
    19681 accaatacta aagccacagg tgtttacttc actagcttac ctgattaagc atctgagttt
    19741 ccttttataa ccttgctttt agcatagcaa atgtaaatgt atcctttatt tggggaacat
    19801 tttaacagta atactctaat gttataacat ttgttctgtg gtcttttgtc gttagtgacc
    19861 taatgcagtt ccttcttccc ataggaatca gtctctgcat ttgcatctca agcactatgg
    19921 tgtgaactgc gatgactggt tattacggct catgtgtggg ggagtagaga ttagaaccat
    19981 ttatgctgcc cataaacagg caaaagcttg cctcatctca agactaagct gtgaacgagc
    20041 tgggacaagg ttcaatgtcc ggggaaccaa cgatgatggt catgttgcca actttgtaga
    20101 gacagagcag gtaacagagt gctgtatatt tcttactgtg atgtaagagg ctcagggagt
    20161 cctaggtaat gggcttgtga gggacacggg acgtgctagg tgatagctac ctggagtttg
    20221 caaatacatt ttggcatgta tagtgttgat gttcttgact acttatgggc cttaagaaag
    20281 gctttcctag aacacaatca cacaggtata aaataatact aattaagtat ttattgttac
    20341 atagtcagtg ctctacattt gacagtgaat tttaaaatct tcaattatag agcaagatgg
    20401 cttaataaaa atattttatg tgaaaataaa ttcaactctt aaagataaat atgtgctacc
    20461 taaaatttta aagttatgac atttggctgt ggaactatgt aactataaag tttcaggcta
    20521 taacccagcc caagtcagat ctttaaatca ggttttagaa actataaaaa ggctaaggaa
    20581 caaattaaaa gaaatataag ggtatatgta taaaacagtg agattaaaaa ggagtttctt
    20641 gtttaaatgt gatttttaca ttttactgtt ttcttgttca atattatcat taactgaaaa
    20701 tgttagtatc ctgttgtggt tgctttgatc aggacagttc tatataaggg gttatcaagt
    20761 gcagagactt cagtagctac ttttctaata cagaattaac aggttttcct ttggattttt
    20821 ttttgtaggt tatatactta gatgactgtg tctcttcctt catacaaatc cgaggatctg
    20881 ttccattgtt ctgggagcaa ccagggttgc aagtatgtgc ctgacggcca gttgttttgt
    20941 tgttattgtt gttttctttt gaaaatttcc acattgtcca aattttcagc atagtccttc
    21001 agtttccctg atgatgctat tgtgtggagt gtaagtgagg ctctgcagaa catggtatct
    21061 tagacatggt tccttcagtt ctgtattagc tgcttttaga gcactgcagt gatttatcgt
    21121 aatgaggcca attttataaa gaaaaaagtc ttatttttat tttggctcat aattttagag
    21181 atcggaagtc acgattggac aggcccattc ctttggcaac tggcaaagca gtgtgacact
    21241 tgacggcatt accacaggtt atatagggaa atgtcgttaa acaggaagca tatagaaagt
    21301 cgcatatata gctcaggctt ctgtaactac tggctccctt aagagtgcac cacaggaaac
    21361 cagcattctc tcccagggca tttctcccca tagtctaatg actcccacaa ggccctactt
    21421 cttaacagtt ccacaccatc accaacactg ccgccctggg caccaaacct ccaaagcatg
    21481 aagacttaag ggacagacca ctttcaaacc atgtaactgt agcaactcag gagcctgttt
    21541 aagtttttaa atttcaagga ctaaaaaatg ttttcattat tattttctca ttattttagt
    21601 atagaatgag ttactctttt aaagcaggag agtttggtag ctagaaagca gtctctgttc
    21661 ctggacttag ttacacccct ttccctatgt accattgcta gctgggtgat cttgcacaag
    21721 agatcgaatt cctctgtctg tctcagtctc ctcatctgta aatgagaatg atggcagtac
    21781 tgtctccttc ctgggattgc tgtgtagact ttaaagattt actttattaa t
//