LOCUS       91578#pL1L2GT1_LF2A_nEGFPOT2A_CreERT2_pAbActNeopA#L3L4_pD223_DTA_T_spec        21937 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2GT1_LF2A_nEGFPOT2A_CreERT2_pAbActNeopA
COMMENT     design_id: 91578
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000027012
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            8691..8945
                     /db_xref="ENSEMBL:ENSMUSE00000305800"
                     /rank="2"
                     /note="ENSMUSE00000305800"
     misc_feature    4275..9789
                     /note="5 arm"
     misc_feature    9817..9841
                     /note="B1 Gateway"
     misc_feature    10808..10825
                     /note="FCHK QC critical region"
     misc_feature    10792..10807
                     /note="FCHK QC prefix region"
     misc_feature    10826..10831
                     /note="FCHK QC suffix region"
     misc_feature    10954..11005
                     /note="F2A"
     misc_feature    10831..10953
                     /note="1Dleader"
     primer          10677..10698
                     /vntifkey="27"
                     /dnas_title="seq primer FCHK2"
                     /label=seq primer FCHK2
                     /note="seq primer FCHK2"
     exon            10777..10830
                     /vntifkey="61"
                     /dnas_title="mouse En2 exon"
                     /label=mouse En2 exon
                     /note="mouse En2 exon"
     misc_feature    11877..11877
                     /note=" A to G to kill RsRII site"
     misc_feature    11827..11880
                     /note="self-cleaving' 2A peptide"
     misc_feature    11881..12906
                     /note="Cre"
     misc_feature    11011..11100
                     /note="3X NLS"
     misc_feature    11101..11817
                     /note="EGFP"
     primer          complement(9970..9991)
                     /vntifkey="27"
                     /dnas_title="LRPCR LAR3_EN2"
                     /label=LRPCR LAR3_EN2
                     /note="LRPCR LAR3_EN2"
     intron          9902..10776
                     /vntifkey="21"
                     /dnas_title="mouse En2 intron"
                     /label=mouse En2 intron
                     /note="mouse En2 intron"
     primer          complement(9866..9889)
                     /vntifkey="27"
                     /dnas_title="FRT3_oligo"
                     /label=FRT3_oligo
                     /note="FRT3"
     misc_feature    9854..9901
                     /vntifkey="21"
                     /dnas_title="Frt"
                     /label=Frt
                     /note="Frt"
     misc_feature    14167..14174
                     /vntifkey="21"
                     /dnas_title="polylinker"
                     /label=polylinker
     misc_recomb     14135..14166
                     /note="Rox(inv)"
     misc_feature    14127..14134
                     /vntifkey="21"
                     /dnas_title="RE sites"
                     /label=RE sites
     primer          14067..14085
                     /vntifkey="27"
                     /dnas_title="seq primer NF"
                     /label=seq primer NF
     primer          13941..13964
                     /vntifkey="27"
                     /dnas_title="LRPCR RAF5"
                     /label=LRPCR RAF5
     misc_feature    13884..14126
                     /vntifkey="21"
                     /dnas_title="SV40 pA"
                     /label=SV40 pA
     misc_feature    12916..13860
                     /note="ERT2"
     misc_feature    15374..15374
                     /note="G to C conservative mutation to kill RsrII site"
     misc_feature    15722..15965
                     /note="SV40pA"
     misc_feature    15780..15803
                     /note="RAF5"
     misc_feature    15529..15548
                     /note="neoBstB1_5"
     misc_feature    15236..15254
                     /note="neoSphI_5"
     misc_feature    14899..14918
                     /note="neoPstI_5"
     misc_feature    14730..15715
                     /note="neomycin resistance "
     misc_feature    14580..14599
                     /note="betact5"
     promoter        14184..14704
                     /note="human beta actin promoter"
     insert          13881..15960
                     /source="pL1L2_GTIRES_BetactP FLFL MapVerified"
     misc_feature    15961..15965
                     /note="for bActNeo"
     misc_recomb     15967..15998
                     /note="Rox"
     misc_feature    16011..16031
                     /note="B2 Gateway"
     misc_feature    9790..16061
                     /note="Synthetic Cassette"
     exon            16440..16537
                     /db_xref="ENSEMBL:ENSMUSE00001312458"
                     /type="targeted"
                     /rank="3"
                     /note="target exon 1 ENSMUSE00001312458"
     exon            17245..17418
                     /db_xref="ENSEMBL:ENSMUSE00001263181"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 2 ENSMUSE00001263181"
     misc_feature    16062..17879
                     /note="Critical Region"
     misc_feature    17903..17936
                     /note="Downstream LoxP"
     misc_feature    17880..17959
                     /note="synthetic loxP region"
     exon            18350..18584
                     /db_xref="ENSEMBL:ENSMUSE00001292356"
                     /rank="5"
                     /note="ENSMUSE00001292356"
     exon            20802..20945
                     /db_xref="ENSEMBL:ENSMUSE00001281655"
                     /rank="6"
                     /note="ENSMUSE00001281655"
     misc_feature    17960..21937
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggatatttc gatattttta tatctgctga tgcctgtttg gcgactgatt
     4321 gtatggtaaa ttttggagaa ggtaccatga ggtcctgagt agaaggtata tccttttgtt
     4381 ttaggataaa atgttgtgta gatacctgtt caattcattt gtttcataac ttctgaagga
     4441 agttttcttc tgtaattttg ttgaagatat ttactgtccc tttaagttga aaatcttcct
     4501 tcttgtctat gcctattatc cttatgcttg gtcttttcgt tgtgtcttgg gttttctaga
     4561 tgtttggggt taggatcttt ttgcatttag cattttcggt gattgtagtg tcaatgcttt
     4621 ttatggtatc ttctgtacct gaaattcact cttctatctc ttgtattctg ttggttatgc
     4681 ttgcatcaat gtctcctgac ttctttccta ggttttcgag agtggtctcc ctttgtgatt
     4741 tctttattgt tttacttcca tttttagatc ctggatggtt ttgttaaatt ctttcacctg
     4801 attggatgtg ttctcctgta tttctttaag ggagttattt atttccttct tatagtcatc
     4861 catcgccatc atgagaagtg actttagatc catatcttgc ttttctggtg tgatggtata
     4921 tccaggaatt gctatggtgg gagagtttgg ttctgatgat acaaagtaac cttggtttct
     4981 gttgcttctg ttctgatgct tgcctcctac catctgatta tctcaaatgc ttgctgccct
     5041 caatatatct gattggagcc tgtccttcct ataatccagg ttgattccaa acacctcaga
     5101 gtccagcttt ctctgggatc ctgtgattct gggatcctgt gaacctgaga ttctgggtgt
     5161 gtcagagttc ttagcagtca agcttcctct gagaccctga gttcctggtg tgaccaagct
     5221 cctgggatcc tggaatccta agacccttgt catattacag tgcctggaag tgttaactcc
     5281 tctgggtaca atggggttgt ctgctgtgtt caaaacctag gtagaccagc accaactaaa
     5341 aggaactcta tacgctggtc agtcaggggg cacctgtgcc cttgcttctg ctgtcacatg
     5401 cccctcgtga ttgttttggg accaatgttg agttccactc accagtgatc ctaggatcct
     5461 ggaagttcta ggtctcctgc agcatggaga gttctctggg gactgtggga ccatctgctg
     5521 actttgcacc caaggtggtc tggagctggc gctgactaga gggaacccaa acagcttctt
     5581 taatgggttt cctgtgtccc tgttcctgct tgcacaggcc cctcgctatt ttttttggaa
     5641 cagatgttgc atttcactca ccaatgatcc taagaacctg ggtgtggaga gtcctctggt
     5701 aactgtgggc ctgtccacag agtttgcaca caaggcggtc taggaccggt gcagactgga
     5761 gtgaacctct ctatctcttt ctaaaccaaa aaaaaaaaaa accaaaaaaa aaaaaaaaaa
     5821 aaaacctccc cccaaaatgt caagcgaaat aaatctaact agaaaataaa aaactagaaa
     5881 gaagcgcaaa aaaacatatg cacacaagta aatctaatat aaaaataaaa ttaaaagcta
     5941 taatataatc aaaatataag taatattaaa aaaagcaata tggaacaaaa tgtcaaaata
     6001 tatacacttg aatttatttt gtattggaca ctgacagctg ggcatgtgat gaaccttaag
     6061 tgtggtttgt atatccaatg agagtccatt ggagaaagct attttttgtg tggggggtta
     6121 gttttattaa ttttatttat atatcataga atacacgtat acatacacaa actcataaga
     6181 aagatgcaag ctttgtaaaa atgaattgaa agggccttct tccctttttt aagatatttt
     6241 tattagatat tttcttcatt tacatttcaa atgctatccc taaagtcccc tatactctcc
     6301 ccccaccttg ttcccctacc cactcactcc cacttcttgg ccctggtgtt ccactctact
     6361 ggggtatata aagtttgcta gaccaagggg cctctcttcc cactagggag aaagctaatt
     6421 tttaagtcat ataatctact agtagaataa gtagttcgtt atttattatt attattatta
     6481 tgtggtaaga atgttgaaac aggttctccc tatatatcca agtctgcctc atatctgtag
     6541 ccatccttct gtctcagttt tccaagtttg agaattctga tgcgaaaatc attacaccta
     6601 ttagtttcta tagcttttgt aaaatgcttg aagttcctgt ttataagacc caatctgttt
     6661 ctctagaatc agctgcttcc atttttttct gatttctaga attctcaata ttttatggaa
     6721 caaaagtata gagggtaact ttttttctgg tcatagtaaa atttaggtta tttgtgacca
     6781 tcgaatatga tgttaaacat ggcgaattac acgccacaga catatatgta ttttttttat
     6841 aattttaatc tctttagtcc ctactattga tcacccattc agcactgttc ttcttctttt
     6901 gaatataaaa attactataa cagagaacca aagctcaggc caaatagatg cttcaacact
     6961 aacctgagtt ttatttacat tatcaaagtg tgagatgaca tttatagact atcatccaag
     7021 accaacatct cttcatctct tctatataat agagacactt taaacacagt ttttcctctt
     7081 ttctatgtcc tgaaacatat tactagtatc tttccaagta tctctttacc aaaagacatg
     7141 gaacaccata tgctgttata aaagagtgtc tctaacatgt gagctaacat gttctgttta
     7201 atttatagta tttttatgat ttgatcaatt aatagctaag ttctctgtgt atagggtgtc
     7261 ttactaatat tcttttcaca aagatttttt tgtgtgacat ttttggaaaa acattcatct
     7321 aaattaattg gtgcaatggc aaatcagcaa atttttattg ttatagagcc tatgaaatgg
     7381 ttgagtatgt attatctttt tttaaaatat tttttattag gtattttcct tatttacatt
     7441 tccaatgcta tcccaaaagt cccactcccc tagagtatgt attatcttaa tttatatact
     7501 ttatagtaac ttgttaatga actaatatta tatttatttg tgaagatcat aaaataagta
     7561 atattattac tgtacaggct tactgtgaag gtccggtata tcaatatgaa caaaaatctc
     7621 aacctacaca ttttacctca cagtggtttt gttgtctttt attgtttatt ttttgggggg
     7681 tgggtgggta cttccacgtt tagtcataag tgtagagtag tgttatacct attgagttaa
     7741 ctgatattag gtactttttt atgtattata attcctatgt gtctttttgc agaataaatg
     7801 cccagaaaat gctgaaaaga aaatacaaaa caataaagaa aataagcatt gtgatctggg
     7861 ccaaagccct tgtttactat gtagttaaat attcttttac tatgccattt tcatgtgaaa
     7921 gttattttga cattttcact aagtaattaa tatggcaatc ttaagtgaga attacttaaa
     7981 tattctgtct ttatatatac ttcatgctca aatcttattt taaaaattaa aattaaaaat
     8041 ggtactttga tatctctgtt ttactaccct ataaaaatgt atccatgcta aggagtaata
     8101 tataactctt aattttgcta taccagaata ttaatattta taatatattg atttctaatt
     8161 tctgtttatg aacaaaatga aataattaga aattttgatg catttgttta taattaatgt
     8221 ttaaaaataa atcaattaat gtattaagca caaacagctg agtacaatat aagtttaata
     8281 taattagaat aaaaagcata ctgctttaac tgaactaaat acaaaggagt gactctacag
     8341 actagtgaga acaatttcct gtcttgacaa gagctttgcc ttcccccaaa aacacacatt
     8401 ctcttcagaa ttgaccaaga cattggtaaa aatcaaacca atgaatgacc aatgctatac
     8461 aataaagaga agcagctact gaaaagcctg catagtgatg ccctaataag catagactgc
     8521 tgcaaacttg caaggttaac ccatttctcc aggtacaatg aaataggcat gtagcttcct
     8581 ggtaaatggg aaaagagagc ttgtttttta gaaaagttgt tagctcacaa agactaagtt
     8641 gattccaagc tctacctgtt gtagttgaac aagtcttttg tctctgacag aaaacaaaca
     8701 ccctgacaaa ccacttaagg tgttggaaca gctgggcaaa gaagtcctta cggagtacct
     8761 agaaaaatta gtacaaagca atgtactgaa attaaaggag gaagataaac aaaaatttaa
     8821 caatgctgaa cgcagtgaca agcgttgggt ttttgtagat gccatgaaaa agaaacacag
     8881 caaagtaggt gaaatgcttc tccagacatt cttcagtgtg gacccaggca gccaccatgg
     8941 tgaaggtaag agcagaatgt actgtgaaca acagctcagg gtgctcatga tctgttcgat
     9001 tttcaggccc aacacttgct ttggtctgct aatctttgtg tcactatcac ttttctccaa
     9061 aatagataat actttagaaa gtgtgttgac tttcataggt aggtctcaga atccaacgtg
     9121 gtaaaaaagc taagtccttt gttcaagcta ttttgagaaa tagctagtca tctgacaatt
     9181 ataaaatgga acactaatcc taaagtttat aaattttgtg gctctcatca gttttagctt
     9241 atggaaaatg ggtgccttct aatactgtct ctcatgtttt tccaaagtta gaaacatagt
     9301 caagccatga acacaacttc ccaaatgctg gctctcatcc aaggtaattt tctggactac
     9361 atactcactg aacactgcct ccatagttaa tatttctgag cacatgttcc atttttttaa
     9421 tgaaatgaac acctgtaaaa ctaaacagtc ttcttccatt aagccaatta gatgcattca
     9481 cacaaactga aagaatctca agcataaaac taaactaaaa aaatattata tccaataagg
     9541 atcagaattg gaggaaggtg ttcaaaatac ttttagtaat ttcaggaatt ccttacatgt
     9601 tatatcaatt aatagggttt cttatcagtt tatacgaatc aaaaattaaa aatttatcta
     9661 cattcctatg ttttcaaatt tcttcccctc caagactgta gctaggagcc acttgagcat
     9721 agtaacgcat gccaaaatgg caaaaatctg tgatgcattg cactgaagag taagagtatt
     9781 actaaaatta aggcgcataa cgataccacg atatcaacaa gtttgtacaa aaaagcaggc
     9841 tggcgccgga accgaagttc ctattccgaa gttcctattc tctagaaagt ataggaactt
     9901 cgaacccttt cccacaccac cctccacact tgccccaaac actgccaact atgtaggagg
     9961 aaggggttgg gactaacaga agaacccgtt gtggggaagc tgttgggagg gtcactttat
    10021 gttcttgccc aaggtcagtt gggtggcctg cttctgatga ggtggtccca aggtctgggg
    10081 tagaaggtga gagggacagg ccaccaaggt cagccccccc cccctatccc ataggagcca
    10141 ggtccctctc ctggacagga agactgaagg ggagatgcca gagactcagt gaagcctggg
    10201 gtaccctatt ggagtccttc aaggaaacaa acttggcctc accaggcctc agccttggct
    10261 cctcctggga actctactgc ccttgggatc cccttgtagt tgtgggttac ataggaaggg
    10321 ggacgggatt ccccttgact ggctagccta ctcttttctt cagtcttctc catctcctct
    10381 cacctgtctc tcgacccttt ccctaggata gacttggaaa aagataaggg gagaaaacaa
    10441 atgcaaacga ggccagaaag attttggctg ggcattcctt ccgctagctt ttattgggat
    10501 cccctagttt gtgataggcc ttttagctac atctgccaat ccatctcatt ttcacacaca
    10561 cacacaccac tttccttctg gtcagtgggc acatgtccag cctcaagttt atatcaccac
    10621 ccccaatgcc caacacttgt atggccttgg gcgggtcatc ccccccccca cccccagtat
    10681 ctgcaacctc aagctagctt gggtgcgttg gttgtggata agtagctaga ctccagcaac
    10741 cagtaacctc tgccctttct cctccatgac aaccaggctc ccaggtcccg aaaaccaaag
    10801 aagaagaacg cagatcgcag atcgcagatc tctagagtca ccgagttgct ttaccggatg
    10861 aagagggccg aaacatactg tccaaggccc ttgctggcaa tccacccaac tgaagccaga
    10921 cacaaacaga aaattgtggc accggtgaaa cagactttga attttgacct tctcaagttg
    10981 gcgggagacg tcgagtccaa ccccggaccg gtgcatgtgg accccaagaa aaagcggaag
    11041 gtggacccta aaaagaaacg caaagtggat cctaagaaaa aaagaaaggt gcacgtgtcc
    11101 atggtgtcca agggcgagga actgttcacc ggcgtggtgc ccatcctggt ggaactggat
    11161 ggcgacgtga acggccacaa gttcagcgtg tccggcgagg gcgaaggcga cgccacatac
    11221 ggaaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ttggcctacc
    11281 ctcgtgacca cactgaccta cggcgtgcag tgcttcagca gataccccga ccatatgaag
    11341 cagcacgact tcttcaagag cgccatgccc gagggctacg tgcaggaaag aaccatcttc
    11401 tttaaggacg acggcaacta caagaccagg gccgaagtga agttcgaggg cgacaccctc
    11461 gtgaacagaa tcgagctgaa gggcatcgac ttcaaagagg acggcaacat cctgggccac
    11521 aagctggagt acaactacaa cagccacaac gtgtacatca tggccgacaa gcagaaaaac
    11581 ggcatcaaag tgaacttcaa gatccggcac aacatcgagg acggctccgt gcagctggcc
    11641 gaccactacc agcagaacac ccctatcggc gacggccctg tgctgctgcc tgacaaccac
    11701 tacctgagca cccagagcgc cctgagcaag gaccccaacg agaagaggga ccacatggtg
    11761 ctgctggaat tcgtgaccgc cgctggcatc accctgggca tggacgagct gtacaagcct
    11821 gcaggcgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccgggccg
    11881 tccaatttac tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc aacgagtgat
    11941 gaggttcgca agaacctgat ggacatgttc agggatcgcc aggcgttttc tgagcatacc
    12001 tggaaaatgc ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg
    12061 aaatggtttc ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc
    12121 ggtctggcag taaaaactat ccagcaacat ttgggccagc taaacatgct tcatcgtcgg
    12181 tctgggctgc cacgaccaag tgacagcaat gctgtttcac tggttatgcg gcggatccga
    12241 aaagaaaacg ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga acgcactgat
    12301 ttcgaccagg ttcgttcact catggaaaat agcgaccgct gccaggatat aaggaatctg
    12361 gcatttctgg ggattgctta taacaccctg ttacgtatag ccgaaattgc caggatcagg
    12421 gttaaagata tctcacgtac tgacggtggg agaatgttaa tccatattgg cagaacgaaa
    12481 acgctggtta gcaccgcagg tgtagagaag gcacttagcc tgggggtaac taaactggtc
    12541 gagcgatgga tttccgtctc tggtgtagct gatgatccga ataactacct gttttgccgg
    12601 gtcagaaaaa atggtgttgc cgcgccatct gccaccagcc agctatcaac tcgcgccctg
    12661 gaagggattt ttgaagcaac tcatcgattg atttacggcg ctaaggatga ctctggtcag
    12721 agatacctgg cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc
    12781 gctggagttt caataccgga gatcatgcaa gctggtggct ggaccaatgt aaatattgtc
    12841 atgaactata tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct gctggaagat
    12901 ggcgatctcg agccatctgc tggagacatg agagctgcca acctttggcc aagcccgctc
    12961 atgatcaaac gctctaagaa gaacagcctg gccttgtccc tgacggccga ccagatggtc
    13021 agtgccttgt tggatgctga gccccccata ctctattccg agtatgatcc taccagaccc
    13081 ttcagtgaag cttcgatgat gggcttactg accaacctgg cagacaggga gctggttcac
    13141 atgatcaact gggcgaagag ggtgccaggc tttgtggatt tgaccctcca tgatcaggtc
    13201 caccttctag aatgtgcctg gctagagatc ctgatgattg gtctcgtctg gcgctccatg
    13261 gagcacccag tgaagctact gtttgctcct aacttgctct tggacaggaa ccagggaaaa
    13321 tgtgtagagg gcatggtgga gatcttcgac atgctgctgg ctacatcatc tcggttccgc
    13381 atgatgaatc tgcagggaga ggagtttgtg tgcctcaaat ctattatttt gcttaattct
    13441 ggagtgtaca catttctgtc cagcaccctg aagtctctgg aagagaagga ccatatccac
    13501 cgagtcctgg acaagatcac agacactttg atccacctga tggccaaggc aggcctgacc
    13561 ctgcagcagc agcaccagcg gctggcccag ctcctcctca tcctctccca catcaggcac
    13621 atgagtaaca aaggcatgga gcatctgtac agcatgaagt gcaagaacgt ggtgcccctc
    13681 tatgacctgc tgctggaggc ggcggacgcc caccgcctac atgcgcccac tagccgtgga
    13741 ggggcatccg tggaggagac ggaccaaagc cacttggcca ctgcgggctc tacttcatcg
    13801 cattccttgc aaaagtatta catcacgggg gaggcagagg gtttccctgc cacagcttga
    13861 tgaagatctg agctcttaat taatgatcat aatcagccat atcacatctg tagaggtttt
    13921 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
    13981 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
    14041 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
    14101 caatgtatct tatcatgtct ggatccgttt aaactaactt taaataattg gcattattta
    14161 aagttacata tggcggccaa ttcgccttct gcaggagcgt acagaaccca gggccctggc
    14221 acccgtgcag accctggccc accccacctg ggcgctcagt gcccaagaga tgtccacacc
    14281 taggatgtcc cgcggtgggt ggggggcccg agagacgggc aggccggggg caggcctggc
    14341 catgcggggc cgaaccgggc actgcccagc gtgggcgcgg gggccacggc gcgcgccccc
    14401 agcccccggg cccagcaccc caaggcggcc aacgccaaaa ctctccctcc tcctcttcct
    14461 caatctcgct ctcgctcttt ttttttttcg caaaaggagg ggagaggggg taaaaaaatg
    14521 ctgcactgtg cggcgaagcc ggtgagtgag cggcgcgggg ccaatcagcg tgcgccgttc
    14581 cgaaagttgc cttttatggc tcgagcggcc gcggcggcgc cctataaaac ccagcggcgc
    14641 gacgcgccac caccgccgag accgcgtccg ccccgcgagc acagagcctc gcctttgccg
    14701 atcctctaga gtcgagatcc gccgccacca tgattgaaca agatggattg cacgcaggtt
    14761 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
    14821 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
    14881 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
    14941 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
    15001 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
    15061 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
    15121 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
    15181 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
    15241 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
    15301 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
    15361 ggctgggtgt ggccgaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
    15421 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
    15481 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
    15541 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
    15601 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
    15661 gcgcggggat ctcatgctgg agttcttcgc ccaccccccg gatctaagct ctagataagt
    15721 aatgatcata atcagccata tcacatctgt agaggtttta cttgctttaa aaaacctccc
    15781 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
    15841 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
    15901 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
    15961 gatccctaac tttaaataat gccaattatt taaagttagg aattaattca cccagctttc
    16021 ttgtacaaag tggttgatat ctctatagtc gcagtaggcg gtctggtaag taaattattt
    16081 ggtaaaggta aagttgaaat gaacaagaat gaggattgaa ataaatgaac aagagtaaaa
    16141 tcatcagaaa tttaaagttg tcgagagtgg aagtcaggtt tgattccaaa ttacaatggt
    16201 gttctcccaa gaattaccag aactcagggt ctcattgttg ggctgttctg cttttcacct
    16261 caaccatctc cttctgccat cttagttgta caatatcctg tgctattgat taccaatagt
    16321 aattgttata taccaaagtg ctatcttcac ttagttttct taaagaccat tctgcccagc
    16381 caacaaatga gggatctaca tcagactgaa acatttacat catatgatgt attttacagc
    16441 taatctggaa atggaggaac cagaagaatc attgaacact ctcaagcttt gttcccctga
    16501 agagttcaca aggctttgca gagaaaagac acaagaagta ctctcctttg ttgttgaaat
    16561 agagacacca aaccaattgt tcatactaaa gacaatttct tagatctctg agcccaagag
    16621 atggtgatat aactgctata gattatgatc ttgattataa gcaagatcaa tttttctttt
    16681 aatgcagtga aaaaaatcat ggtgttaatt attttattta ttttttctct cctgttcatg
    16741 agttatgaaa attttttctg cagtcatggt atcaaaatat ctataaaact acacagttga
    16801 ctaatagtta tctgtatact ggtttaaaaa acacaaatac tgaagaaggt ttattgttac
    16861 agtatataga atataatatt gatatatcaa aactggtatc acaataatag tagtagatgc
    16921 aattttttaa taattttctt gacatgagct gatatctttg gattttatag ttaagctatc
    16981 ccttacattc tagggaactt tgactaggta ctaaggaaga gagtagtaat tagcctggat
    17041 tttttttaag acatgtgtta actatcccag ttatattctt gttctgtctt tagcccttga
    17101 gaaggacaca gacagtatct ttcagttata gtttgaggga gaatgaagct atataagacc
    17161 ttaaggaagc aatacttatg ataatcaatt aggctttcaa acaactcaag taagccaaat
    17221 tcattgtatg tctaatttaa acagatttac ccaataaagg aggccaatgg ccgtacacga
    17281 aaggctctta tcatatgcaa tacagagttc aaacatctct cactgaggta tggggctaac
    17341 tttgacatca ttggtatgaa aggccttctt gaagacttag gctacgatgt ggtggtgaaa
    17401 gaggagctta cagcagaggt aagagccact gcaacatcct atactgggaa cacgaagagt
    17461 tttaatcaca gatgagcatt ataatgttaa ggagaaactc aagtgaaggg gtgtgtgtgt
    17521 gagagagaga gagagagaga aagagagaga gagagagaga gagagagaga gagagagaag
    17581 aagaagaaag aaagaaagaa agaaagaaag aaagaaagaa agaaagaaag aaagaaagaa
    17641 agaaagaagg aaagaaggaa agaaggaaag aaggaaagaa ggaaagaaag aaagaaagaa
    17701 agaaagaaag aaagaaagaa agaaagaaag atcaaaatag tcaaattagg taaatgaaat
    17761 ccgtgccttt taaagttaca aatatttcat gtaccataaa aaagcgcctg gcagtgtggg
    17821 tttatgcagt atttttaatt ccaaagagga gcaattgaga ctcagtggcc gaacatgatg
    17881 agatggcgca acgcaattaa tgataacttc gtatagcata cattatacga agttatggtc
    17941 tgagctcgcc atcagttcag ctataagaaa aacttcaaag aatggagtat gtgtttatct
    18001 acataactaa aattgaatga acattcattc aaactgatag gtgctaaagg aaagggatgg
    18061 gagttggaaa gtagagtgca ataaaagata agaccatcag aaaaccttag tgaagttaat
    18121 gaagatactg aaatgcatgt actgagagca aggggctctg aaaaggtgtg agcagagaga
    18181 ctttcaagtt gagaggtggt gcataagcta catctagact gcatggtgca agaagaaagg
    18241 gaaagtatca gatttttttt gtaaatttcc aatgttgatt ttaccgaaat gaggaggtta
    18301 agatttatcc taactgtcat cccactaacg aagtcatcga tttttccagg gcatggagtc
    18361 agagatgaaa gactttgctg cactctcaga acaccagaca tcagacagca cattcctggt
    18421 gctaatgtct catggcacac tgcatggcat ttgtggaaca atgcacagtg aaaaaactcc
    18481 agatgtgcta cagtatgata ccatctatca gatattcaac aattgccact gtccaggtct
    18541 acgagacaaa cccaaagtca tcattgtgca ggcctgcaga ggtggtaagt tctttggtgg
    18601 aaccacgaac tcccacgctg ctggctatgc aggtgctggc agggtaatgc cacatgggct
    18661 ttgtgtctag aagaattaac tttaggctta atttctattt agttttcaga aattaatgga
    18721 tctaatagga aatgtgccat tacaaaaatt tgcctggtta gttgaaagat tttcatgtaa
    18781 cttttaccag tagaagtgat taagaacttt tccagaatcc ccaagtcaat tgagacaagt
    18841 atacaaatat tagagcaaaa tggttttatt ttctactata cttgtggttt ttgtattatc
    18901 attttgaaat ttatgtttga aagtgagaca atacaattgt tgtatctgag ttcagtaccc
    18961 tactcttact aatgaacaga aaataggacc atacagaaat aaggtcagca atgaaacacc
    19021 agggttatag taaatcaagt gggaatcaat aatatctaca gaatattgta ataaacatat
    19081 acagaatata catttacaat tccagctttg gggagaaaag gaatggtaga ttccagtgga
    19141 agaccccaca gcccaatggc caaatatcac aatgtatttg ttaagttttt ggctagtata
    19201 taaaatgttt gtataataca tttactactc accaagatta ccattcaaaa tatccctcac
    19261 actcatgttt ttttaaggac atagccaatt atacaattag cctatgaaac catcaacaca
    19321 tgcctggata aagaaaatgt ctttatgcat aattaagtac tataaaacca taaaaaaaat
    19381 aatttcctga aatttgaagg aaaataaaaa tggaactgta tatcttcatg ttaagcaaga
    19441 tgaatcaatc acagaatgat gactgtccaa tgttattttt cagtttctta tacatggaaa
    19501 ctaaataaaa gtataaaaat gtgatctgaa gatataagag tgtttgtaag taaggaattg
    19561 aggtcgtgga gctggggaat atagcaagaa gggagagtga aaagagatag agggagtcaa
    19621 ggcaaatgag agcagcatgg tcaagggaaa tgtgtccatg tgtgaaagta cactatgtta
    19681 cattcctgct gcagtcactg cacgaataaa ccagtatata aaaatccaat acttgaatgt
    19741 gctcagatgt aattctgtct tttactttat cctgtacagg taatataaaa taagtgcttc
    19801 attatgatat tctatacact tatacttgac ttgatcagtc tataccctac caacctttgc
    19861 tttctctatt cctctcttgc tcctccatat cttttattga aacattttca ctttcacctt
    19921 tgtgtgtctc tttagttttt aatctatgaa aagatgaaaa ctaatctaag actagattta
    19981 agtaaaatca tctaaaattg tcttcccaat ctggcttatt ttgcttaaca tgatgacccc
    20041 aagtttaacc catttacatt caaataataa aatttggatc tcttttcatt gcataatgct
    20101 ttcttgtcca tattaacctc attgttctta tacattcaat tattaatcgg tacctatgct
    20161 gatgtcataa tttggatatg atgaatagta aaacaataca tatgggtgta tacatatgtc
    20221 tattgtatat tttattttac tgagtttcac tgtatatttc caacttgtca ggaacttaca
    20281 gtaatctcct accttagtct cttgggagct gagattatag ttgtgtgctt tcacatccaa
    20341 attgtaggat gcagataggc ctatatgact ttgaattcat atagccatgg gcctataagt
    20401 aactaaagta gaaataactt ttatataacc aaatggtata aaatgatgca gatataaaac
    20461 tcctctgctg cttttagtaa acacgtcctt acaactttat gactctgcct ttattttttc
    20521 tcatcatgta acatttctca atgatcctag agggaaagaa attctctctg ctctgtctct
    20581 gtctctgtca ctctctgtgt gtgtgtatgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt
    20641 gtgtgcgcgc gcgcgcatgc gcgcgcgtgc atatgctcct acttacagtg cttgtatgta
    20701 tatatatgtg aaactgatct gagctcttaa aatggctgag gtaggaaact ggaaatctta
    20761 ctcagtgtga tcagtgctag attgcacttt ttttccccca gggaactctg gagaaatgtg
    20821 gatcagagag tcttcaaaac cccagttgtg cagaggtgta gatctaccta ggaatatgga
    20881 agctgatgct gtcaagctga gccacgtgga gaaggacttc attgccttct actctacaac
    20941 cccacgtatg tattttctaa accatatagc tttgcaggtc aaaagacatc tttatatagc
    21001 aatggtctaa cacaaatttt gagggtaaca atttacatta attaaatcat atatttgttt
    21061 tgagtacatc tactcctaga cacaactttc aagactggcc ttttcctata gttaaggatt
    21121 tgtatagtga aagatatata tattccagtc cacggatcca ggggatcctc ttcacagctt
    21181 ttaacataca tgattaattt gattcatttc ttttaatcaa attattttag cattcttaca
    21241 taaaaagtta tagatcaaag aattatcagc ctcttcccaa atattcagcc ccaaggtcat
    21301 tttggccaac atcctgccaa attctagata taccaatttt atgacatggt ccactaggaa
    21361 tggggtatcc tgttaaagaa gttgtagaaa acacattcac aggtctcaag gtggggtata
    21421 ttgctgagct tttgatctct gtatgtgttt cctaaagtgc tttatgtcta ctttttgaat
    21481 ttgtatattt ttcatggctt gttttaaaaa actattttgt gaaggcaaac ttgataaact
    21541 caaacagcac aggtttaatg tctacactta atgaatttga aaatatatgt aagacaccat
    21601 caggacagtg tatgacatct acttacctct ttcttctcaa agtatatcag tcttacgtat
    21661 tttccttgct gagcatattt tgtatgaaaa cattatacat ctaacctact gatcacactt
    21721 tacagatcag tattattatt ggtgatgcaa atgtaataca tgtaaataca acagacccta
    21781 tgacttattc atcttctgta cctcaaatat tggttctgta tagtattttg ttaattatat
    21841 agtaaaaaag caaagaacca atcaacaaaa cacaatgaca taatctatag acataataaa
    21901 tgttatctat cagtaggaag tgggttactg tacaaaa
//