LOCUS       45104#pL1L2_GT1_LF2A_nEGFPO_T2A_CreERT_puro#L3L4_pD223_DTA_T_spec        20987 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT1_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 45104
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000058921
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            7292..7385
                     /db_xref="ENSEMBL:ENSMUSE00001220884"
                     /rank="3"
                     /note="ENSMUSE00001220884"
     misc_feature    4275..8590
                     /note="5 arm"
     misc_feature    8618..8642
                     /note="B1 Gateway"
     misc_feature    8655..8702
                     /note="Frt"
     misc_feature    8703..9202
                     /note="mouse En2 intron"
     primer          complement(8771..8792)
                     /note="LRPCR LAR3_EN2"
     primer          complement(8667..8690)
                     /note="FRT3"
     primer          12868..12886
                     /note="seq primer NF"
     misc_feature    12928..12935
                     /note="RE sites"
     misc_feature    14728..14761
                     /note="loxP"
     misc_feature    complement(12936..12967)
                     /note="Rox"
     misc_feature    complement(14688..14719)
                     /note="Rox"
     misc_feature    12968..12989
                     /note="polylinker"
     CDS             13548..14144
                     /note="puromycin N-acetyl transferase"
     misc_feature    13663..13668
                     /note="Mutated RsrII site"
     intron          9203..9577
                     /note="mouse En2 intron"
     misc_feature    9575..9577
                     /note="Splice Acceptor"
     exon            9578..9631
                     /note="mouse En2 exon"
     primer          9478..9499
                     /note="seq primer FCHK2"
     misc_feature    9632..9754
                     /note="1Dleader"
     misc_feature    9755..9806
                     /note="F2A"
     misc_feature    9902..10618
                     /note="EGFP"
     misc_feature    9812..9901
                     /note="3X NLS"
     misc_feature    12684..12922
                     /note="SV40 polyadenylation site"
     misc_feature    11717..12661
                     /note="ERT2"
     promoter        13000..13511
                     /note="PGK promoter"
     misc_feature    14255..14687
                     /note="PGK pA"
     primer          12742..12765
                     /note="LRPCR RAF5"
     misc_feature    10628..10681
                     /note="T2A"
     misc_feature    10678..10678
                     /note="A to G to kill RsRII site"
     misc_feature    10682..11707
                     /note="Cre"
     misc_feature    9593..9632
                     /note="FCHK lf2a_lacz_gt1"
     misc_feature    9593..9608
                     /note="FCHK QC prefix region"
     misc_feature    9609..9626
                     /note="FCHK QC critical region"
     misc_feature    9627..9632
                     /note="FCHK QC suffix region"
     misc_feature    14784..14804
                     /note="B2 Gateway"
     misc_feature    8591..14834
                     /note="Synthetic Cassette"
     exon            15242..15410
                     /db_xref="ENSEMBL:ENSMUSE00001206850"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 1 ENSMUSE00001206850"
     exon            15755..15833
                     /db_xref="ENSEMBL:ENSMUSE00001240128"
                     /type="targeted"
                     /rank="5"
                     /note="target exon 2 ENSMUSE00001240128"
     misc_feature    14835..16004
                     /note="Critical Region"
     misc_feature    16028..16061
                     /note="Downstream LoxP"
     misc_feature    16005..16084
                     /note="synthetic loxP region"
     exon            17633..17893
                     /db_xref="ENSEMBL:ENSMUSE00000692630"
                     /rank="6"
                     /note="ENSMUSE00000692630"
     misc_feature    16085..20987
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggactaatt tccttgcact ttctgattgt tggtttatta ttttatgatc
     4321 ctaagtcacc agcgtagtgc atatggagcc tcaggacttg cgtcagtcac tccctacctt
     4381 gctgtgaagt tgcccactgt gtccaccttc cctcagcaga ccagcgcctg cctctgaagc
     4441 ttctctgctc tgctctgctc gagcactctc acctctggct tttttgtttt gttggtcttt
     4501 tgctgctcaa tatactcctt ttaaaattta aaggtatgat taacattcta ggcagctttg
     4561 ctgcctcatg aatacctatt gcatcctttc catattttag ttctttgcta aatttttcaa
     4621 gatctttcat ctccttgaac atagaaagca gacctattag tgactattta tggtggttct
     4681 ctttcatgta accttacctc tgtatgtttg gctggtgtgt gtgtgtgtgt gtgtgtgtgt
     4741 gtgtgtgtgt gagagagaga gagagagaga gagagagaga gaatgtgtgt tgtaatttac
     4801 atttacaatt gtacaagtaa gttgaagtct ataatacatt taggagaata tgttattctt
     4861 ctacaaagcc tttaattctg taatagaatt aaactgatgc tctgtttgcc aggcaaaaca
     4921 gaaattttaa ttttagatga ataatgcatt taatataagg tctaaatatt acataagatt
     4981 cattttaaaa ttacatcaca aggctggtca atggtagagg gaatatgcaa agctcctgat
     5041 ttggtttcca gcactggaaa caaattttac atttataatt caagtttaac tgagtgttgt
     5101 ttatttgtat ttgctaaatc tggcacacca agccatgcgt cattagttaa ctaggctctg
     5161 tgcatctcga atgacactaa ctgtgggaga atgggagcat tgggaagcag gctctccggt
     5221 ttcttcttgg acagtagacc ctgctgtgag aggcccaggc tgagaaaggc ttctcccagg
     5281 agctgtaagc tccagcagtt ctgcatctga tgcactccag ggactggggc ggctgaggcc
     5341 agctgcagcc cctcgagagc tgctccactt ttgtcagaag ctttgctggg ctttccctcc
     5401 ttcgtctttc ttgaattggc aggtgccctt ggggaagaaa tggcctacat gtcggggcca
     5461 cctctttgag tttttgtata gatcttggcc tcgtagtttc tcactatcct gctagttctt
     5521 tgatgcattt gtacagattt ttttttttta actgtcgttt tccaagttct ttcagcagag
     5581 agttggccta aattactgaa tctgccatta ctggaagcag tgtaaaattt aaaaacatgc
     5641 cagaaagggg catggttgga ctcgaactcg aggaccaggc ttcaggaata tgaaatactc
     5701 tccttgggta ggaaaggaag catatatttt agactgtgct taaattatgt tgttctaaag
     5761 gaaagtataa attcagttta ggtaaggaaa gagtgtgtgt atatgtgtat gtttgtgtgt
     5821 gtgtgtgtgt gtgtgtgtgt gtgtgtatgt gcccacctcc ccccactgca cgcgcatgta
     5881 tggtgtattt gtacatgtat gtggctgcaa atagcaaata cacaaatttg cataagccag
     5941 aaggggatgt catgtgtcct gctctgcttt tctcttcctt atttttttaa gacagagttg
     6001 ctcactgaat ctggagctag gctgatggcc agcggcaagc ccagtactgg ggttacagac
     6061 atgtctggcc gtgcctgact ttctgtatga aatctaggaa tttgaactca gttcctaatg
     6121 cagtgctctt accactgagc tatctccctg acctagttaa cagattattt atttttatta
     6181 ttttatttat ttacagttca aatattatcc cccttcccag ttttccttcc acaagctcct
     6241 tacactctcc cccttccctc ttgcctctgt cagggtgctc cctcacccac ccacccattt
     6301 ccaccttacc accctaacat tcccctatgc tggggcatca agccttcaca ggaccaaagg
     6361 gttccccttc cactgatgct agataaggca gtcctctgct gcatatgcag ctggagccat
     6421 gggtcacccc atgtatatac tctttggttg gtggtttagt ccctgggagc tttggaaggt
     6481 ctggttggtt gatattgttg ttcttcctat gaggttgcaa accccttcag ctccttcagt
     6541 ccttgcccta attcctccat tggggtctct gtgtcagtcc catgtttggc tgcatgcatc
     6601 cacatctgta ttggtcaggc tcaggcagag cctctcagga cagcaatatc agactcctta
     6661 atgtggaaac tagcaagctt gagttctaac cagttaggaa atatgttaac cacttaggtt
     6721 tcagcccttg gactgatata atctagttgt ttggatagaa cagtaaataa ggtaatgatt
     6781 tttgttggta atatttaaat cagaatatag tgatgctgcc tttatatatt ttctggctcg
     6841 tctacagtga atgtaatgtg aagacatgaa ttaggaaatt ggttgtcacc tacatggtat
     6901 tctacgttac tgtgtggacc tctctgctat ctacaaaata gctcccagct tcttctagag
     6961 ttacactaat gcatgtgaga gcattgccag gtcatttcct aaaaagaatt gtccctagtt
     7021 ctcagttttt agttctttga cactgctctc attttctatc cctaaggact actaactgat
     7081 ttccagacca cactcagaac ttcaggagga atggtttcac tccttccgtt tcagtctttt
     7141 gccccagaga gtagattggt ttctgtggtg accaatctct aagtggaaca gtgataaaac
     7201 aaaccattaa acacttttaa ttttttacta gctgtaacaa aaatcatttc aagcatgtct
     7261 tatgttcgtg tttctgaaaa ttctttttaa gagttacgaa attgcaactg ccctagaaaa
     7321 tcgaagccac aaggttcggt attctgatac actggaaagt ggatcgattg tattttctct
     7381 gtctggtaca gtatatttgt taattgtctt tactcagggt ttttgtttgt ttgtttggta
     7441 aaatatgtgg atgcacatgc taatttttgc agttcagtag tgacataatc tcttagtgtg
     7501 ttatcttttg aaagagtaaa tggttgcact ttgtgcttgc tgcagatgta gtgccaactg
     7561 tgggatagat ttgttccttc agttaaatgg tgtctggtag aagatgggga aagcacttgc
     7621 tttggaacca aggtaaaaat agaaacgtcc acttgcttag caataaatat aagttgttaa
     7681 cagaggccaa cagtactact ctggccataa tttacatacc caaaccaata tatgatatac
     7741 tcctattact ttgatttact gaaaaaataa atattgaaga actttatagg gacaaataaa
     7801 tgttcctaga attacaaaat tatgtatgag acatgaacaa agttcaggag ttaagttagg
     7861 ctaaaaaagt ctggtgatat ggagggacag cctccatctg tgcatgggga gtaacaggtg
     7921 tgacagaagt gatgaaggaa ggcaggcatc agtctgtgcc ctctcactca caagctagcg
     7981 accctaatgc cgagcctgcg ccacagggag accatcgcag aatctcaaat agttctgtta
     8041 ggtttaccat tctagcagag attttagaag gcaggtgatt tctgaatcat ctaaaaaggc
     8101 atgctgaaaa ttatccagac tccaaagcat tcctcctagt tgaatagaaa atactccaga
     8161 aaaaaaagag catttttggt gacttgtatg tttggttatc atctgtatgt ttacaaagag
     8221 gtactctttc catgttcttg aggaggacaa tgatgttcag agaggttata taaaatgtat
     8281 aaatgcattc agtcactgtg taatcacaca cagctttcta ttgatcgtgt ctttttactt
     8341 tctgaacagc tattactaag ctcaagtgtc ctttaagaca attctgatag ttggtcccat
     8401 ttcagacacc aggtatatgc ttgtttgaat acattcagtt agttacggct cctcctgagg
     8461 gaggaaggtt tcttcaataa gaaataattg aagatttgct ttaaactccc tttctcttat
     8521 tgattctcac tttgacagtc attacagaac cttctgagtc gctgaacact tgcttgtaag
     8581 gtagtggttg aaggcgcata acgataccac gatatcaaca agtttgtaca aaaaagcagg
     8641 ctggcgccgg aaccgaagtt cctattccga agttcctatt ctctagaaag tataggaact
     8701 tcgaaccctt tcccacacca ccctccacac ttgccccaaa cactgccaac tatgtaggag
     8761 gaaggggttg ggactaacag aagaacccgt tgtggggaag ctgttgggag ggtcacttta
     8821 tgttcttgcc caaggtcagt tgggtggcct gcttctgatg aggtggtccc aaggtctggg
     8881 gtagaaggtg agagggacag gccaccaagg tcagcccccc ccccctatcc cataggagcc
     8941 aggtccctct cctggacagg aagactgaag gggagatgcc agagactcag tgaagcctgg
     9001 ggtaccctat tggagtcctt caaggaaaca aacttggcct caccaggcct cagccttggc
     9061 tcctcctggg aactctactg cccttgggat ccccttgtag ttgtgggtta cataggaagg
     9121 gggacgggat tccccttgac tggctagcct actcttttct tcagtcttct ccatctcctc
     9181 tcacctgtct ctcgaccctt tccctaggat agacttggaa aaagataagg ggagaaaaca
     9241 aatgcaaacg aggccagaaa gattttggct gggcattcct tccgctagct tttattggga
     9301 tcccctagtt tgtgataggc cttttagcta catctgccaa tccatctcat tttcacacac
     9361 acacacacca ctttccttct ggtcagtggg cacatgtcca gcctcaagtt tatatcacca
     9421 cccccaatgc ccaacacttg tatggccttg ggcgggtcat cccccccccc acccccagta
     9481 tctgcaacct caagctagct tgggtgcgtt ggttgtggat aagtagctag actccagcaa
     9541 ccagtaacct ctgccctttc tcctccatga caaccaggct cccaggtccc gaaaaccaaa
     9601 gaagaagaac gcagatcgca gatcgcagat ctctagagtc accgagttgc tttaccggat
     9661 gaagagggcc gaaacatact gtccaaggcc cttgctggca atccacccaa ctgaagccag
     9721 acacaaacag aaaattgtgg caccggtgaa acagactttg aattttgacc ttctcaagtt
     9781 ggcgggagac gtcgagtcca accccggacc ggtgcatgtg gaccccaaga aaaagcggaa
     9841 ggtggaccct aaaaagaaac gcaaagtgga tcctaagaaa aaaagaaagg tgcacgtgtc
     9901 catggtgtcc aagggcgagg aactgttcac cggcgtggtg cccatcctgg tggaactgga
     9961 tggcgacgtg aacggccaca agttcagcgt gtccggcgag ggcgaaggcg acgccacata
    10021 cggaaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cttggcctac
    10081 cctcgtgacc acactgacct acggcgtgca gtgcttcagc agataccccg accatatgaa
    10141 gcagcacgac ttcttcaaga gcgccatgcc cgagggctac gtgcaggaaa gaaccatctt
    10201 ctttaaggac gacggcaact acaagaccag ggccgaagtg aagttcgagg gcgacaccct
    10261 cgtgaacaga atcgagctga agggcatcga cttcaaagag gacggcaaca tcctgggcca
    10321 caagctggag tacaactaca acagccacaa cgtgtacatc atggccgaca agcagaaaaa
    10381 cggcatcaaa gtgaacttca agatccggca caacatcgag gacggctccg tgcagctggc
    10441 cgaccactac cagcagaaca cccctatcgg cgacggccct gtgctgctgc ctgacaacca
    10501 ctacctgagc acccagagcg ccctgagcaa ggaccccaac gagaagaggg accacatggt
    10561 gctgctggaa ttcgtgaccg ccgctggcat caccctgggc atggacgagc tgtacaagcc
    10621 tgcaggcgag ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccgggcc
    10681 gtccaattta ctgaccgtac accaaaattt gcctgcatta ccggtcgatg caacgagtga
    10741 tgaggttcgc aagaacctga tggacatgtt cagggatcgc caggcgtttt ctgagcatac
    10801 ctggaaaatg cttctgtccg tttgccggtc gtgggcggca tggtgcaagt tgaataaccg
    10861 gaaatggttt cccgcagaac ctgaagatgt tcgcgattat cttctatatc ttcaggcgcg
    10921 cggtctggca gtaaaaacta tccagcaaca tttgggccag ctaaacatgc ttcatcgtcg
    10981 gtctgggctg ccacgaccaa gtgacagcaa tgctgtttca ctggttatgc ggcggatccg
    11041 aaaagaaaac gttgatgccg gtgaacgtgc aaaacaggct ctagcgttcg aacgcactga
    11101 tttcgaccag gttcgttcac tcatggaaaa tagcgaccgc tgccaggata taaggaatct
    11161 ggcatttctg gggattgctt ataacaccct gttacgtata gccgaaattg ccaggatcag
    11221 ggttaaagat atctcacgta ctgacggtgg gagaatgtta atccatattg gcagaacgaa
    11281 aacgctggtt agcaccgcag gtgtagagaa ggcacttagc ctgggggtaa ctaaactggt
    11341 cgagcgatgg atttccgtct ctggtgtagc tgatgatccg aataactacc tgttttgccg
    11401 ggtcagaaaa aatggtgttg ccgcgccatc tgccaccagc cagctatcaa ctcgcgccct
    11461 ggaagggatt tttgaagcaa ctcatcgatt gatttacggc gctaaggatg actctggtca
    11521 gagatacctg gcctggtctg gacacagtgc ccgtgtcgga gccgcgcgag atatggcccg
    11581 cgctggagtt tcaataccgg agatcatgca agctggtggc tggaccaatg taaatattgt
    11641 catgaactat atccgtaacc tggatagtga aacaggggca atggtgcgcc tgctggaaga
    11701 tggcgatctc gagccatctg ctggagacat gagagctgcc aacctttggc caagcccgct
    11761 catgatcaaa cgctctaaga agaacagcct ggccttgtcc ctgacggccg accagatggt
    11821 cagtgccttg ttggatgctg agccccccat actctattcc gagtatgatc ctaccagacc
    11881 cttcagtgaa gcttcgatga tgggcttact gaccaacctg gcagacaggg agctggttca
    11941 catgatcaac tgggcgaaga gggtgccagg ctttgtggat ttgaccctcc atgatcaggt
    12001 ccaccttcta gaatgtgcct ggctagagat cctgatgatt ggtctcgtct ggcgctccat
    12061 ggagcaccca gtgaagctac tgtttgctcc taacttgctc ttggacagga accagggaaa
    12121 atgtgtagag ggcatggtgg agatcttcga catgctgctg gctacatcat ctcggttccg
    12181 catgatgaat ctgcagggag aggagtttgt gtgcctcaaa tctattattt tgcttaattc
    12241 tggagtgtac acatttctgt ccagcaccct gaagtctctg gaagagaagg accatatcca
    12301 ccgagtcctg gacaagatca cagacacttt gatccacctg atggccaagg caggcctgac
    12361 cctgcagcag cagcaccagc ggctggccca gctcctcctc atcctctccc acatcaggca
    12421 catgagtaac aaaggcatgg agcatctgta cagcatgaag tgcaagaacg tggtgcccct
    12481 ctatgacctg ctgctggagg cggcggacgc ccaccgccta catgcgccca ctagccgtgg
    12541 aggggcatcc gtggaggaga cggaccaaag ccacttggcc actgcgggct ctacttcatc
    12601 gcattccttg caaaagtatt acatcacggg ggaggcagag ggtttccctg ccacagcttg
    12661 atgaagatct gagctcttaa ttaatgatca taatcagcca tatcacatct gtagaggttt
    12721 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
    12781 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
    12841 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
    12901 tcaatgtatc ttatcatgtc tggatccgtt taaactaact ttaaataatt ggcattattt
    12961 aaagttacat atggcggccg ccctaaggct cgacctcgaa attctaccgg gtaggggagg
    13021 cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct
    13081 acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc
    13141 cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc
    13201 ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc
    13261 tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc
    13321 aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg
    13381 gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcttcc ggaggcccgg
    13441 cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg
    13501 gcctttcgac ctgcatccat ctagatctcg agcagctgaa gcttaccatg accgagtaca
    13561 agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg
    13621 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccagatcgc cacatcgagc
    13681 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
    13741 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
    13801 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
    13861 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
    13921 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
    13981 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
    14041 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
    14101 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc ccgccccacg
    14161 acccgcagcg cccgaccgaa aggagcgcac gaccccatgc atcgatgata tcagatcccc
    14221 gggatgcaga aattgatgat ctattaaaca ataaagatgt ccactaaaat ggaagttttt
    14281 cctgtcatac tttgttaaga agggtgagaa cagagtacct acattttgaa tggaaggatt
    14341 ggagctacgg gggtgggggt ggggtgggat tagataaatg cntgctcttt actgaaggct
    14401 ctttactatt gctttatgat aatgtttcat agttggatat cataatttaa acaagcaaaa
    14461 ccaaattaag ggccagctca ttcctcccac tcatgatcta tagatctata gatctctcgt
    14521 gggatcattg tttttctctt gattcccact ttgtggttct aagtactgtg gtttccaaat
    14581 gtgtcagttt catagcctga agaacgagat cagcagcctc tgttccacat acacttcatt
    14641 ctcagtattg ttttgccaag ttctaattcc atcagaagct ggtcgagtaa ctttaaataa
    14701 ttggcattat ttaaagttag gcgcgccata acttcgtata gcatacatta tacgaagtta
    14761 tgtcgagata tttaaatatt agacccagct ttcttgtaca aagtggttga tatctctata
    14821 gtcgcagtag gcggacttat atcctttggg atagcttttc taataagcca gaaaatattt
    14881 tcaaaaaata ttctagtatg aaaacttttt aaagttttca ttctgtagtc ctggatggtt
    14941 gtttctatag cctggaatgg ccttggactc accacagttc tcctgtccaa gcttccatat
    15001 gctgagatta tagatgtgta tcaccacacc tggcttgaaa ggttaacttt taaaatagac
    15061 ttatggcaga aaaaaattac tggccaatgt ttttccttta aatatttaga acaggtcatt
    15121 ctgttttcta gagcatgtca aagaaatatc caaaccattt tgttatatac aaattgtttg
    15181 aggtccctga tattattgag cttgaggctc tgtgtaactt catcatattc accctttcta
    15241 ggagttgcat tcttgttgat ggatgctaag gagtgtatga catcggctga ggaaatattt
    15301 gtaaccaaaa ttgagaagtt tattaacatt caccaaaata gtttcttggt tctatttgct
    15361 ccactccatg ggcctgaaga atggagtctc atgttcagga tacatcagag gtatggaagc
    15421 aaagcacagc cagtctttct tagttttgtt tacttcagtt cttaatgctt ttaggcagtc
    15481 aaactttata acctgttata tgtaacccaa cagggcagac aggtgttcct taagtcaggg
    15541 tgaccagagt tggtgttgac atggggcctg cctattttac ataagcaaaa caaagtgagc
    15601 tctaaaacct ggttacatag ccacctcaaa tcttctaagg cacaaagcag gatgtgactg
    15661 aatgaacatg tgtacagcag agctctgtcc cctcctcgtg ctgcctgtga cagcaaacag
    15721 cagtcagtca gttacgtaac cactttcttt tcaggttctt gggcagtaac ttacgaatac
    15781 ttcctgtaca caacacagta aacgctcttg accttatgtg cactatagcc aaggtaagcc
    15841 acctggacaa aggatactca tacacaaata tgtacatctt ggccagagat tttaaggttt
    15901 tgattttaaa taaagttttg agataataga gaatttcccc cctccatttg cttcttaatg
    15961 cccccccccc atgctctttt agatgtggcc tctttttgtt acgtgagatg gcgcaacgca
    16021 attaatgata acttcgtata gcatacatta tacgaagtta tggtctgagc tcgccatcag
    16081 ttcaggtttc ctgtagttct tgtcagacct ggggcttttt acttacatgt tatcattcct
    16141 attgctggtg actttttgag tcatgtttag ccatctatgt tgagacttca tatgtatagc
    16201 ttctgacatt tctaggagac acaatctcac agccaatgcc ctattcttga atcaaaatct
    16261 ttctgacctc ttttgcaatg atcactgagc cttaggtgta ggagttgtgt tgtagttgta
    16321 tcagttaaaa ctagtaggac ccacaaccct gcattttgat tggttgtggt tttctgtaat
    16381 gttcttttct attttgcaga gacaagtttc cttgatgagg ggtgaggact atacttacct
    16441 gtggatatga caaatattta aagtgtagtt aggtatactg ctggtttagt aaagtggtgg
    16501 ttgtatgttt ccctccaaga tccatgacat cactagtcct gggtagtttt cagggttccc
    16561 agtacaggca tgatttccct cttgtttagc aggtcttaaa tctaattaga gacctgttcg
    16621 ttcctgctaa gatatgcatg ccactgctgt acccttaggg ttatcatgtc atgctagtca
    16681 ctgttgtggt tcatagacat actgggtagg attgttggtt ccttccctca tttggaagct
    16741 tgcaccgccc cttctagggt cataaaggta gtcttcagaa ataaggactt accttccacc
    16801 cgtgggcaac caagagcaat agcaatagct tctaatgttt tgggagtctc ttggacaact
    16861 ctgacaacca atgcaaagga gggcttctca tgtctagtgt tgggttgtgt tagatggcct
    16921 atggctcttc aatgactgtc agccccggtg ggaaaacttc attaaaatta tagatttata
    16981 taccgactta agtgcaatat aggtatttta aggtagactc tttatgactt tttaagatac
    17041 tgttattgtt actaagggct tttgttttta aatatgtctt caagttgtgt accctcttga
    17101 ggattgtcaa gctatccacc ttctactcat ttctttcctg agcctgacat cttcgcagtt
    17161 tattcctggt aaccttgtga actgttcccc accagttcag tctcttctca ggtggcttct
    17221 ccttgacagt gttcatagac acgtcaactt cccttcctaa gttttgcact tggaaatcct
    17281 agagcatttc tcatactgca ttttagggag ttacagtctg aaattccttg aagacacagt
    17341 tcagttactc tatcactcta attcatacac aatgttttgg cacatccatg tcgagagatg
    17401 tttgctgaat tatcatttga ggcagaattt cattctgcag ccctgggtgg cctaaaattc
    17461 tccatgtaga caatactggc ttcagtcaaa ctcagacatc cacatgcctc taacacctaa
    17521 gttctgggat caaaggtgtg tgtcatcctc cagcagaaag aattttgcaa aatacagtcc
    17581 taccaaaata ctctttctat aaataatgct gtatgtttac atcttttttc agactacgtc
    17641 caaaccacac atagacagca tctgctacag aatgataaca actaaagcgt acatcataga
    17701 gcagagccct gtctggagaa cacttcaaaa gataaagttg agtagtgact cagttagtgc
    17761 agactcagga gagtgatcac actttttcaa atggaggatt agttaaactt attaacttgt
    17821 aatgtccaaa tgtagattta agggcgtctg tatttaaaat aatatgtata caattaaact
    17881 tattttattt gggaagcttg gttgaatggt tcctttgatg tttaggagtg cagctgcaca
    17941 acacagtgct accctagcat atactggacc ttttaaacac tacagggaaa aaaaaaagag
    18001 aaaataatgg atgcatagat aaaactcata gttttgtttg ttttgttttg ttttagagac
    18061 agggtttggt gtttttgttt gcttgtttgt ttttctcatg tataacagcc ctggctgtcc
    18121 ttaagctcac tttgtagatt aggccgacct tgaactctct acctctgctg ggattagagg
    18181 tgtacaccac catgcctgtg tgtatataca tgatatatac atgtcccaca tgggtaccag
    18241 ctgtcgtgga ggccagagga gggtgttgcc tcctctgcat ctgagttgca gttgtaacct
    18301 ccacgtgggt gctgagaact ggaactaggt tctctggaag agcaggaagt actcttaacc
    18361 accgagtgat ctctccaggc ttttaaaagc aaagactatt tactactgat atgtatcaga
    18421 aacttgcaag ccaaaaaaaa aaaaaaaaaa gctgtattat taaaaagaat tatgcaattt
    18481 ttctagtagt aaaatttctg taaatatttt tatggtggta taaagtataa tgaaaaacag
    18541 cattttgttg gtttttattt atgaaataga acctttactt tatcccaaca ttttcttcag
    18601 aggtagactt tgatttgatt tcaatgagtt cttctactcg agccagaaca ctttcaatca
    18661 agtcaaatgt gaagagagcg gaatgtaaga cctgaaaagt caagagcatc atgttgtcat
    18721 cactggtcta gtgttaaaca acccagacca gccttcagct cacagtcctc ctgcatcaga
    18781 ctggccactg caggggttcc aggaccatga tcccacccca gcagacatct gtgttattag
    18841 tgctgcctct aaaacaaagc ataccagaga ctttaggctg gagatagctt aaaatttgtg
    18901 ggttacctta agctgcattt caggaggcat agaagggatt tcttctccac ctacagtcac
    18961 ggaacaaaca cctttacaca ttgaggctac tgtagggaag aaataaagca tctcacattt
    19021 agccattaca acagttgagg gtttcagcac cgtgtgtgtg cgcgcgcgcg tgtgtgtgtg
    19081 tgtgtgtgtg tgtgtgtagt actgggaatc aaacccaggg tgtgttgaac atactaggcc
    19141 aatgctttac tgctgattcc cagccccagc tctcagactg tagtacatgt atttattctg
    19201 tgtgtacatg tgtggcatgg tgcatgtgta gtatgacatg gatttattct gtgtgtacat
    19261 gtgtggcatg gtgcatgcgt agtatgacat ggatttattc tgtgtgtaca tgtgtggcat
    19321 ggtgcatgcg tagtatgaca tggatttatg ctgtgtgtac atgtgtggca tggtgcatgc
    19381 gtagtatgac atggatttat tctgtgtgta catgtgtggc atggtgcatg tgtagtatga
    19441 catggattta ttctgtgtgt acatgtgtgg catggtgcat gtgtagtatg acatggattt
    19501 attctgtgtg tacatgtgtg gcatggtgca tgcgtagtat gacatggatt tattctgtgt
    19561 gtacatgtgt ggcatggtgc atgcgtaggg gtcagagggc gacttggagg agttggttct
    19621 cttcttccac cacttgggtc ccagggattg aatccaggtt ttgggcatga ctgatggcag
    19681 gcacctttac ccactgagct atcttgtcat tttaattgta catagataag ctactttaat
    19741 tgttactaat tgtaaaatgt gctaatgagg taacactttg ttataaacaa ataaaattaa
    19801 gcactagaat taagcgatat gtgactgaat cttcagagaa atggctttat tttggcatgt
    19861 ataggacaca taaaacattt ttctttagta tactcaagag cctataccag gagtgtaaga
    19921 ttactgtacc agaattgcca actttaaaga agtaattctg aatctatatt tcttttttac
    19981 aatttgtaca aatttactct gggacaatga ggggtggacc atgtgatata agagcgtaca
    20041 gtcgggcccg aggagagatg cttgtaatac acccactcac attcagagcc tgggctctaa
    20101 acacagagtt tcaatgtggt ttaagccttc attaacccaa tttcagtact ggctaagaat
    20161 catattaatt acacaaatat tgcttttatg gccctcattt ttgcataaag tgaacatgta
    20221 ctaaaatgtg atagtttggg gctatagaga cgcctcagtg gctaagagta cttactgctc
    20281 taatacagag cctagggtgt ttcccagcac ccacatggag gttcaaaatc acttgtaact
    20341 cctgttccac gtgatcaagc aacctcttct gacctccaca ggttcatgca cacatacagt
    20401 acacataaaa taaatattta aaaaatatgg tcattccaga atttgaatgg ttagacctaa
    20461 tggcaaataa aattgcatat taccaacctt ggttattttg ttggctgttt ttaatttctg
    20521 cttcataatg tgcttttgac atattaagtc ctatatgaag tggctttaga aagttatttt
    20581 caatttttcc aagttctgtc cataatccag tctgttcaaa agatttaatg aacaaattag
    20641 ttacttggtt cagttaataa acatccacca attgtgagaa ataatgatcg tgccaccgtg
    20701 ctgctccgca gtgagttctg atatgtcagt cagctgcacc caccaccccc ccccacacac
    20761 acacacatct tttaaacaac ttctcttaca ctgctgggaa atgcagaggt ctaacaagaa
    20821 actagatata aattcaaact tcaaaaatat acctatctag tccaccagca tctatagatt
    20881 tggaaaaaca aagattaatc tgtaatgctt gcattgtaga gaagacttta aaagcaatgt
    20941 ttaatgaaag agaatgcaag aatatgtctc ataacaggaa aatgtta
//