LOCUS       294#pL1L2GT0_LF2A_nEGFPOT2A_CreERT2_pAbActNeopA#L3L4_pD223_DTA_T_spec        18629 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2GT0_LF2A_nEGFPOT2A_CreERT2_pAbActNeopA
COMMENT     design_id: 294
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000074127
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            6429..6514
                     /db_xref="ENSEMBL:ENSMUSE00001262969"
                     /rank="3"
                     /note="ENSMUSE00001262969"
     exon            5465..5696
                     /db_xref="ENSEMBL:ENSMUSE00001227627"
                     /rank="2"
                     /note="ENSMUSE00001227627"
     misc_feature    4275..7769
                     /note="5 arm"
     misc_feature    7797..7821
                     /note="B1 Gateway"
     misc_feature    8788..8791
                     /note="FCHK QC critical region"
     misc_feature    8772..8787
                     /note="FCHK QC prefix region"
     misc_feature    8792..8797
                     /note="FCHK QC suffix region"
     misc_feature    8920..8971
                     /note="F2A"
     misc_feature    8797..8919
                     /note="1Dleader"
     primer          8657..8678
                     /vntifkey="27"
                     /dnas_title="seq primer FCHK2"
                     /label=seq primer FCHK2
                     /note="seq primer FCHK2"
     exon            8757..8796
                     /vntifkey="61"
                     /dnas_title="mouse En2 exon"
                     /label=mouse En2 exon
                     /note="mouse En2 exon"
     misc_feature    9843..9843
                     /note=" A to G to kill RsRII site"
     misc_feature    9793..9846
                     /note="self-cleaving' 2A peptide"
     misc_feature    9847..10872
                     /note="Cre"
     misc_feature    8977..9066
                     /note="3X NLS"
     misc_feature    9067..9783
                     /note="EGFP"
     primer          complement(7950..7971)
                     /vntifkey="27"
                     /dnas_title="LRPCR LAR3_EN2"
                     /label=LRPCR LAR3_EN2
                     /note="LRPCR LAR3_EN2"
     intron          7882..8756
                     /vntifkey="21"
                     /dnas_title="mouse En2 intron"
                     /label=mouse En2 intron
                     /note="mouse En2 intron"
     primer          complement(7846..7869)
                     /vntifkey="27"
                     /dnas_title="FRT3_oligo"
                     /label=FRT3_oligo
                     /note="FRT3"
     misc_feature    7834..7881
                     /vntifkey="21"
                     /dnas_title="Frt"
                     /label=Frt
                     /note="Frt"
     misc_feature    12133..12140
                     /vntifkey="21"
                     /dnas_title="polylinker"
                     /label=polylinker
     misc_recomb     12101..12132
                     /note="Rox(inv)"
     misc_feature    12093..12100
                     /vntifkey="21"
                     /dnas_title="RE sites"
                     /label=RE sites
     primer          12033..12051
                     /vntifkey="27"
                     /dnas_title="seq primer NF"
                     /label=seq primer NF
     primer          11907..11930
                     /vntifkey="27"
                     /dnas_title="LRPCR RAF5"
                     /label=LRPCR RAF5
     misc_feature    11850..12092
                     /vntifkey="21"
                     /dnas_title="SV40 pA"
                     /label=SV40 pA
     misc_feature    10882..11826
                     /note="ERT2"
     misc_feature    12928..13510
                     /note="synthetic fragment mutates RsRII"
     misc_feature    13340..13340
                     /note="G to C conservative mutation to kill RsrII site"
     misc_feature    13688..13931
                     /note="SV40pA"
     misc_feature    13746..13769
                     /note="RAF5"
     misc_feature    13495..13514
                     /note="neoBstB1_5"
     misc_feature    13202..13220
                     /note="neoSphI_5"
     misc_feature    12865..12884
                     /note="neoPstI_5"
     misc_feature    12696..13681
                     /note="neomycin resistance "
     misc_feature    12546..12565
                     /note="betact5"
     promoter        12150..12670
                     /note="human beta actin promoter"
     insert          11847..13926
                     /source="pL1L2_GTIRES_BetactP FLFL MapVerified"
     misc_feature    13927..13931
                     /note="for bActNeo"
     misc_recomb     13933..13964
                     /note="Rox"
     misc_feature    13977..13997
                     /note="B2 Gateway"
     misc_feature    7770..14027
                     /note="Synthetic Cassette"
     exon            14414..14622
                     /db_xref="ENSEMBL:ENSMUSE00001276554"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 1 ENSMUSE00001276554"
     misc_feature    14028..14872
                     /note="Critical Region"
     misc_feature    14896..14929
                     /note="Downstream LoxP"
     misc_feature    14873..14952
                     /note="synthetic loxP region"
     exon            17950..18067
                     /db_xref="ENSEMBL:ENSMUSE00001301111"
                     /rank="5"
                     /note="ENSMUSE00001301111"
     misc_feature    14953..18629
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggacttttt gttgacagtt ctttttattt cctataatct tcagcactga
     4321 aattccccat atgactttgt gtgacattaa tcatatacat gattgacctc cagtatacag
     4381 acaatcaccc tggggacctg ataaaataca aatcctgact tagtcctcgt gagactgtgt
     4441 ctgagatttc taatgagggc ccaggtgata acatatggcc tgtttagaag caagctttga
     4501 gtaccaaggt tttaagtagt gttcaaggta caactatggt ctgttgtact ctctagtaat
     4561 attatttctc ccttttactt tctatctgta ttccaggcat ggtaagacct ctattttcag
     4621 attttcaatc aaagactgct tcaaaaagtg gtaacagtag ttgtagtaga atctagaaac
     4681 cttccttcag agtacttctg gtaaagaggg gctgtggtaa ttagtaaaca agataggata
     4741 gtgaatatta aaactctgtg aatcatccac tcctcaaaca tcagatagtt tgtgtatgtt
     4801 tgttcataca tagcgttcct gaagaaaata tattaagtcc agaatttaaa atctggctcc
     4861 aaagtagaac atatgcaggt gtgactctgg ttataaacca gtgcgttaag gatgaagtat
     4921 caagatgctg ctcaaaagca aagttgccac ggtacctaca tcgtgacttc gggaggctgt
     4981 tattaatcgg gaggtgatgc tgaaagggat catttttaac cctattttaa aaggcttgga
     5041 tcggtagtga agacgttaga ggctctgaat gtattagaga ggaagaatca tcctgagtat
     5101 ccagggtgtg ccttattgag aacttggaaa taaaaaaaaa aatctgatag caaaccgggg
     5161 gagggggtgg tgtctaataa taccagttgt cactgcaagt atacttggtc tgagcgttgt
     5221 gacatcctaa gttagtggtt ctcaaccttc taatgcagcc accctccaaa cagttgtggt
     5281 gacctccaaa cataaaatta ttttcattgc ttcttcatca ctgtaatttt gctgttgtta
     5341 tgaatcataa tgtaaaatgt ctgatacgag acccctgtga gagtcacaat ccacagagtg
     5401 agaaccgctg tcctaagcca agtattacac agtttgtgta agagtaactg accttctctt
     5461 ccagagtgaa ctcaatgcat acagtcatga agtcattacc atccttggag agattgattt
     5521 tatgttgggg aatcttcctg aattggcttc tgcgagaccg gctaagaaga acctgctaac
     5581 catgatggat gaggcctacg ttcagcccga gcctctggga gtcgtactga ttattggagc
     5641 ttggaattac cctttcgttc ttaccatgca accgctggtg ggagccattg ctgcaggtct
     5701 ggcgccagct gtggtgtctt tattccttgg tcgagagttg tattttgtct tagtgtttgt
     5761 aagtgtctag aatacatttt ctctagtggt cggcagcagc aatttattga gaatccattc
     5821 gtgtatgtac gtggatgagt gctgctgaga agttgaagtt ttccatgact actgaagtac
     5881 ggggggcatg aatctgtgag actcagaggc atctgattga gtcagtagtc ccaagtcttt
     5941 ggagtcaggc attcatattc taatgtggtt tcagtttact ggcctccgtc tttacacaaa
     6001 ccagtctaaa atccaagtct gaccagtttc tttatttaca tgaaggtatt gggcgtaatg
     6061 ctagctagag tgtccagtga atcccaacag gtggaagttc cagatcaacg cttatgttga
     6121 cctagcctgt tagctcaatg agtctgagca gagaggaaac cctccaggca gagctacaaa
     6181 gaaagagata gaaatctggg ggactgtgag gaactggcag atcctgttgt cagtgagatg
     6241 ctgagagcta acgggttagc acttgagaag gaaaacatga acagaaagag gacaggagat
     6301 ctggggggtt ggggggcaga cctaagagct gcaaaaataa gtgaaactta gcctggacct
     6361 gtacatgggg tagctatcaa agttactcaa gtcctggatg atgtcattgt gctatttgct
     6421 ttatttagga aatgctgcca ttgttaagcc ctcagaactc agtgaaaaca cggccaagat
     6481 cttggctgaa ctcctccctc agtacttaga ccaggtaaga gtatcttgat ccatcttcaa
     6541 cacacctgtt tatggtagaa aacacagata caattcttgt gctagtggat gtcattgctg
     6601 gctggggatc aatacgattt ccttaagtcc acaacagttg gcttaataaa ggaagccagg
     6661 ttccaaaaca ccctgtagaa tgacccagca actgtgcttg ctcagtacag tgtaccttgg
     6721 cctgtggctt ttatacatat ctttcatctc tttctaccct ctgccacttg ggaggataca
     6781 attttagaca tggtttattt agtgaaatag ttaatgagaa gctagcatgt tttgatcact
     6841 tggttttttt cctaaccaaa tatcactgtg ttattacatg agtacttctg ttttgtaaag
     6901 aaagtgcctt cctgacccag cactccgagt taacatgcgg gtattgagaa catcaaaggc
     6961 cgactagggg aagtgtccta cccccagttt ttgggtaggg gatcaaattc agaagctctc
     7021 ctgttctcta gagaggaagt tgattgcatt gtgactgcgg ggtcccacgt gcatctgcag
     7081 gggatgttat ctacttagct tttatcaaaa ggcaacttgg aaacccctga tttgtgttat
     7141 atttattaat ttgtaatttc cctgcacccg acttcagtcc ctagagtaat tttgttctgt
     7201 tcattgccgt agcaccattg tcatagctca cagaacagct ctgttctcta gcttggttat
     7261 ccaatgtaga agaatgaaat ggaagatagt gaaggtcata gatcaagtgg gacttggaag
     7321 cagaagacac agggttatta aggccttgat ttgccagatt acctatggag taaggcatgg
     7381 atacattaaa acttacactc gtctgctgga ctctaaagtg aataagatgg aatcagcagg
     7441 agatcagatg agaagcagag attgccaggc cagctcattc atcctccatc tgacagctca
     7501 gacgcccttg tgtatctcat gcttgccttg ccatttcctc tcggtcttta cttgtgtttc
     7561 catgctgtta taattcaagg tcttttctct cggttagcta cctaacggag gcgtagtgca
     7621 tgtaccgggt agggtacaga cgttatgtga catggaactt ttcccaataa caagctcaga
     7681 gagtgtctga agtcactgat gctagtgagg gacagagcct agatccaatc cagatctact
     7741 tcagaaccaa actcctcaga tctggtgcta aggcgcataa cgataccacg atatcaacaa
     7801 gtttgtacaa aaaagcaggc tggcgccgga accgaagttc ctattccgaa gttcctattc
     7861 tctagaaagt ataggaactt cgaacccttt cccacaccac cctccacact tgccccaaac
     7921 actgccaact atgtaggagg aaggggttgg gactaacaga agaacccgtt gtggggaagc
     7981 tgttgggagg gtcactttat gttcttgccc aaggtcagtt gggtggcctg cttctgatga
     8041 ggtggtccca aggtctgggg tagaaggtga gagggacagg ccaccaaggt cagccccccc
     8101 cccctatccc ataggagcca ggtccctctc ctggacagga agactgaagg ggagatgcca
     8161 gagactcagt gaagcctggg gtaccctatt ggagtccttc aaggaaacaa acttggcctc
     8221 accaggcctc agccttggct cctcctggga actctactgc ccttgggatc cccttgtagt
     8281 tgtgggttac ataggaaggg ggacgggatt ccccttgact ggctagccta ctcttttctt
     8341 cagtcttctc catctcctct cacctgtctc tcgacccttt ccctaggata gacttggaaa
     8401 aagataaggg gagaaaacaa atgcaaacga ggccagaaag attttggctg ggcattcctt
     8461 ccgctagctt ttattgggat cccctagttt gtgataggcc ttttagctac atctgccaat
     8521 ccatctcatt ttcacacaca cacacaccac tttccttctg gtcagtgggc acatgtccag
     8581 cctcaagttt atatcaccac ccccaatgcc caacacttgt atggccttgg gcgggtcatc
     8641 ccccccccca cccccagtat ctgcaacctc aagctagctt gggtgcgttg gttgtggata
     8701 agtagctaga ctccagcaac cagtaacctc tgccctttct cctccatgac aaccaggctc
     8761 ccaggtcccg aaaaccaaag aagaagaacg cagatctcta gagtcaccga gttgctttac
     8821 cggatgaaga gggccgaaac atactgtcca aggcccttgc tggcaatcca cccaactgaa
     8881 gccagacaca aacagaaaat tgtggcaccg gtgaaacaga ctttgaattt tgaccttctc
     8941 aagttggcgg gagacgtcga gtccaacccc ggaccggtgc atgtggaccc caagaaaaag
     9001 cggaaggtgg accctaaaaa gaaacgcaaa gtggatccta agaaaaaaag aaaggtgcac
     9061 gtgtccatgg tgtccaaggg cgaggaactg ttcaccggcg tggtgcccat cctggtggaa
     9121 ctggatggcg acgtgaacgg ccacaagttc agcgtgtccg gcgagggcga aggcgacgcc
     9181 acatacggaa agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccttgg
     9241 cctaccctcg tgaccacact gacctacggc gtgcagtgct tcagcagata ccccgaccat
     9301 atgaagcagc acgacttctt caagagcgcc atgcccgagg gctacgtgca ggaaagaacc
     9361 atcttcttta aggacgacgg caactacaag accagggccg aagtgaagtt cgagggcgac
     9421 accctcgtga acagaatcga gctgaagggc atcgacttca aagaggacgg caacatcctg
     9481 ggccacaagc tggagtacaa ctacaacagc cacaacgtgt acatcatggc cgacaagcag
     9541 aaaaacggca tcaaagtgaa cttcaagatc cggcacaaca tcgaggacgg ctccgtgcag
     9601 ctggccgacc actaccagca gaacacccct atcggcgacg gccctgtgct gctgcctgac
     9661 aaccactacc tgagcaccca gagcgccctg agcaaggacc ccaacgagaa gagggaccac
     9721 atggtgctgc tggaattcgt gaccgccgct ggcatcaccc tgggcatgga cgagctgtac
     9781 aagcctgcag gcgagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc
     9841 gggccgtcca atttactgac cgtacaccaa aatttgcctg cattaccggt cgatgcaacg
     9901 agtgatgagg ttcgcaagaa cctgatggac atgttcaggg atcgccaggc gttttctgag
     9961 catacctgga aaatgcttct gtccgtttgc cggtcgtggg cggcatggtg caagttgaat
    10021 aaccggaaat ggtttcccgc agaacctgaa gatgttcgcg attatcttct atatcttcag
    10081 gcgcgcggtc tggcagtaaa aactatccag caacatttgg gccagctaaa catgcttcat
    10141 cgtcggtctg ggctgccacg accaagtgac agcaatgctg tttcactggt tatgcggcgg
    10201 atccgaaaag aaaacgttga tgccggtgaa cgtgcaaaac aggctctagc gttcgaacgc
    10261 actgatttcg accaggttcg ttcactcatg gaaaatagcg accgctgcca ggatataagg
    10321 aatctggcat ttctggggat tgcttataac accctgttac gtatagccga aattgccagg
    10381 atcagggtta aagatatctc acgtactgac ggtgggagaa tgttaatcca tattggcaga
    10441 acgaaaacgc tggttagcac cgcaggtgta gagaaggcac ttagcctggg ggtaactaaa
    10501 ctggtcgagc gatggatttc cgtctctggt gtagctgatg atccgaataa ctacctgttt
    10561 tgccgggtca gaaaaaatgg tgttgccgcg ccatctgcca ccagccagct atcaactcgc
    10621 gccctggaag ggatttttga agcaactcat cgattgattt acggcgctaa ggatgactct
    10681 ggtcagagat acctggcctg gtctggacac agtgcccgtg tcggagccgc gcgagatatg
    10741 gcccgcgctg gagtttcaat accggagatc atgcaagctg gtggctggac caatgtaaat
    10801 attgtcatga actatatccg taacctggat agtgaaacag gggcaatggt gcgcctgctg
    10861 gaagatggcg atctcgagcc atctgctgga gacatgagag ctgccaacct ttggccaagc
    10921 ccgctcatga tcaaacgctc taagaagaac agcctggcct tgtccctgac ggccgaccag
    10981 atggtcagtg ccttgttgga tgctgagccc cccatactct attccgagta tgatcctacc
    11041 agacccttca gtgaagcttc gatgatgggc ttactgacca acctggcaga cagggagctg
    11101 gttcacatga tcaactgggc gaagagggtg ccaggctttg tggatttgac cctccatgat
    11161 caggtccacc ttctagaatg tgcctggcta gagatcctga tgattggtct cgtctggcgc
    11221 tccatggagc acccagtgaa gctactgttt gctcctaact tgctcttgga caggaaccag
    11281 ggaaaatgtg tagagggcat ggtggagatc ttcgacatgc tgctggctac atcatctcgg
    11341 ttccgcatga tgaatctgca gggagaggag tttgtgtgcc tcaaatctat tattttgctt
    11401 aattctggag tgtacacatt tctgtccagc accctgaagt ctctggaaga gaaggaccat
    11461 atccaccgag tcctggacaa gatcacagac actttgatcc acctgatggc caaggcaggc
    11521 ctgaccctgc agcagcagca ccagcggctg gcccagctcc tcctcatcct ctcccacatc
    11581 aggcacatga gtaacaaagg catggagcat ctgtacagca tgaagtgcaa gaacgtggtg
    11641 cccctctatg acctgctgct ggaggcggcg gacgcccacc gcctacatgc gcccactagc
    11701 cgtggagggg catccgtgga ggagacggac caaagccact tggccactgc gggctctact
    11761 tcatcgcatt ccttgcaaaa gtattacatc acgggggagg cagagggttt ccctgccaca
    11821 gcttgatgaa gatctgagct cttaattaat gatcataatc agccatatca catctgtaga
    11881 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
    11941 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
    12001 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
    12061 actcatcaat gtatcttatc atgtctggat ccgtttaaac taactttaaa taattggcat
    12121 tatttaaagt tacatatggc ggccaattcg ccttctgcag gagcgtacag aacccagggc
    12181 cctggcaccc gtgcagaccc tggcccaccc cacctgggcg ctcagtgccc aagagatgtc
    12241 cacacctagg atgtcccgcg gtgggtgggg ggcccgagag acgggcaggc cgggggcagg
    12301 cctggccatg cggggccgaa ccgggcactg cccagcgtgg gcgcgggggc cacggcgcgc
    12361 gcccccagcc cccgggccca gcaccccaag gcggccaacg ccaaaactct ccctcctcct
    12421 cttcctcaat ctcgctctcg ctcttttttt ttttcgcaaa aggaggggag agggggtaaa
    12481 aaaatgctgc actgtgcggc gaagccggtg agtgagcggc gcggggccaa tcagcgtgcg
    12541 ccgttccgaa agttgccttt tatggctcga gcggccgcgg cggcgcccta taaaacccag
    12601 cggcgcgacg cgccaccacc gccgagaccg cgtccgcccc gcgagcacag agcctcgcct
    12661 ttgccgatcc tctagagtcg agatccgccg ccaccatgat tgaacaagat ggattgcacg
    12721 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
    12781 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
    12841 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
    12901 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
    12961 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
    13021 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
    13081 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
    13141 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
    13201 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
    13261 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
    13321 gtggccggct gggtgtggcc gaccgctatc aggacatagc gttggctacc cgtgatattg
    13381 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
    13441 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
    13501 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
    13561 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
    13621 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccccggatc taagctctag
    13681 ataagtaatg atcataatca gccatatcac atctgtagag gttttacttg ctttaaaaaa
    13741 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
    13801 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
    13861 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
    13921 tgtctggatc cctaacttta aataatgcca attatttaaa gttaggaatt aattcaccca
    13981 gctttcttgt acaaagtggt tgatatctct atagtcgcag taggcggaca ctgtgcaaaa
    14041 cagaatgaga gagccacaaa attcccttag agcatccgcc cctcgtctcc cacgtgaccc
    14101 ctgtggggcg cattggctct cagctcccac gtgacctctg tggggcgcat tggctatcag
    14161 tagtgctttc attgatgagg tctcatagga aatactgaac tttccgtcct gcgtagcttg
    14221 gtggttccca gttgccagca gatacgttgt cctccatttt tcttggatca ccgctctgat
    14281 cggtcgttct caggagggga ttttaatctt ttggttttgc tgagtgctac ttatttatgc
    14341 tgggggaaaa aaatctctga catgaggcaa taagattagt ttgacaaagc aggaatctgt
    14401 ttctctcttt aaggacctgt atgcgattgt taatggcggt atcccggaaa ccacggagct
    14461 tctgaagcag cggtttgacc acattctcta tacagggaac actgcagttg gaaaaattgt
    14521 catggaagct gctgccaagc atctgacccc tgtgaccctg gaactcggcg ggaaaagccc
    14581 ttgttacatt gacagagact gtgatctgga cgtggcttgc aggtgggact ggctttgatc
    14641 ctgaggtttt ccctagcggg tggaaggttt gctaatggct tgttttcctt catgcttgaa
    14701 cctcatgact tacagcctca atggtcatca gactttagtg gttggtgtgc tcaggaataa
    14761 ctggagcctc tcgaactgtc ttctgatgcc acacataggg cctgccagca tggcattttc
    14821 cttaggaagt ttggaaatat tcttatggat ataaacctgg caagacattt tggagatggc
    14881 gcaacgcaat taatgataac ttcgtatagc atacattata cgaagttatg gtctgagctc
    14941 gccatcagtt caaatcctga tgttttgctg gttgatgaaa tagatagttc aaagggttaa
    15001 gtgtattagt aaagtgttta aattctcagt tggaagggca ttgtgttcac tactctgctg
    15061 agatctttta ctaacaaaac aatatgacag atattttgaa tagtggtttg catctgggaa
    15121 ccagtatagc ttgttctgcc ggcaggaaac tggaagcccc acctcttcca accagcgatt
    15181 ggctgtgggt atagttattg attgatcaag aaccaaatga ggaacaggac cttcagcatt
    15241 cacaggcagg ttcctgatca gagcatcaga actaacccta catctcatct tttctgtcca
    15301 attacaaaca aacaaacaaa caaacaatct tctcccagac ataaatggaa caaaaccata
    15361 acaatcatgt aaatcacaca gttagtatga cacaccacta acatccagcc agcccatcat
    15421 gtttgtcagt tagtatgaca caccgctaac atccagccag cccatcatgt ttgtcagtta
    15481 gtatgacaca ccgctaacat ccagccagcc catcatgttt gtcagttagt atgacacacc
    15541 gctaacatcc agcccatcat gtttgtcagt tagtatgaca caccgctaac atccagccca
    15601 tcatgtttgt cagttagtat gacacaccgc taacatccag cccatcatgt ttgtcagtta
    15661 gtatgacaca ccgctaacat ccagcccatc atgtttgtca gttagtatga cacaccacta
    15721 acatccagcc agcccatcat gtttgtcagt tagtatgaca caccgctaac atccagccag
    15781 cccatcatgt ttgtcagtta gtatgacaca ccgctaacat ccagccagcc catcatgttt
    15841 gtcagttagt atgacacacc gctaacatcc agccagccca tcatgtttgt cagttagtat
    15901 gacacaccgc taacatccag cccatcatgt ttgtcagtta gtatgacaca ccactaacat
    15961 ccagcccatc atgtttgtca gttagtatga cacaccgcta acatccagcc catcatgttt
    16021 gtcagctaga taaagtactc tactatcatt cctaacttta aaaattatga tcctatccct
    16081 ggattatgtt tagattttag cctgttacac catctgaaaa acacatcttc cactctataa
    16141 cctctctcaa tggtccccat caaagcatca gaaacaaccc gtacaaacca gtggcacacg
    16201 cctttagtcc cagcagaggc aggcggattt ctgagttcga ggccagcctg gtctacaaag
    16261 tgagttccag gacagccagg gctacacaga gaaaccctgt ctcaaacaaa caaacaaaca
    16321 aaaaaaagtt cagcccctct tcactaaatt aggtttgact gcaagggaat gactgtctcc
    16381 atgctctggg tgataaaatt ttctcttctt cagagctgtg gcaccgtgga ggagataggg
    16441 agttaggtca tggttgccgc ttggcctctt agaatagagt agaacatcaa gaaaaagaga
    16501 ccagtggtga gtgctaggtt ttgtgctagg gctttgtctg tatgtaggaa gggggagggt
    16561 ctacaaagag gagcctggta gaggatgctg gctatgcctt ctgcacctgc acagctggcc
    16621 agcaatactg cagccacagt ggaaaactgg aacctggtct gtgctcactc actatcccaa
    16681 atggcctttt ggctggataa gcaagggagg agtgttggga ggccactctc ttttcctgca
    16741 ggccaagtct tcatagcttg ttaggtactg tttaccagga agactcgttt tcttgctact
    16801 tagaatattt tacctccgtt gaaaatgcgt tctaaaggct ggcgagatgg ttcagtgggt
    16861 caacatactt gatgcacaaa cctgaccatc tgagtttgat acctggagct catgtttcta
    16921 aaaagccaga ttctgagtag atgggaggcc tcaagtatgg agcgagcaga gcagcagaaa
    16981 tgagagatgc tgcatcagtg gggtagaatg agagaaccag ctcccaggat gttgtcgtct
    17041 ggcctacaca tgtgtgtgtg tgtgtgtgtg tgtgtacgtg tgtgtgtgtg tgtgtgtgtg
    17101 tgcgcgcgtg cgcatacaca ctggcatgca tgcttccatg tacacacatg cacacacaca
    17161 aaatcgaaaa gaaacctgaa aatgcattct aagttttaac tagatgatct acagctccag
    17221 tttttattta tcttgttttt aacaattact gagtatacag aaccctatcg gttggcatga
    17281 tggttaacct tagctgtcaa ctggatggca tttagaatta ccgtgaaaac aaatctctgg
    17341 gcttgtcttt gagggattat ccagatcagg ttgattgaag tagggagacc caccctaagt
    17401 ctggggttat gggtgaaaca aaagtcaaac tgagcataag aattggccgc tcgctccttc
    17461 ctggcggtgg gtgtcatgtg accagacatc aagctcctgc caccatgact tcctgccctg
    17521 atgtagtacc cttcaactgt gagccaaagc cagctcttcc ttcctgaagt tacctttgtc
    17581 agatgttttg ttaaagcaac gtgtaaagta actaatccat tggaagacat taaaatggaa
    17641 acaatggggg cgggagggtg gctctgcagt tatgagcact cttggttaag aagctgctct
    17701 tcccagcgat cctgagttta attcccaaca cccacatgat ggctcacagc catctgtaat
    17761 ggaatctgat tccctcttct ggtgtgtctg aagacagatc actcatatac attaaaaaaa
    17821 gaaaggaagg aaggaaggaa ggaaggaagg aaggaaggaa ggaaggaagg aaggaaggaa
    17881 ggaaggaaaa gagactgaat tgtatagata atctgttttt ggtttgtttt tgtttttttt
    17941 tctccttaga cgcatagcct ggggaaagta catgaattgt ggtcaaacct gcattgctcc
    18001 tgactatatc ctgtgcgaag cctccctcca gaatcaaatc gtacagaaga ttaaggaaac
    18061 ggtgaaggtc tgtgtccagc aattgcgatc ccactgattt cagtagggtg atcgtcgagt
    18121 gaagtacttg cagacagcat ctctgttcac ctctactccc aacccagaca accccagtca
    18181 cacgtgtgac tgcattcact cccgggctag ctgagaaccc tgcatctgca ggggtcaagg
    18241 ctgagttgat gcatgtccac ttaaatcagg ttattgattt gttgacaatt agtggaggca
    18301 tatagttaaa tgtcagtgtt gcctgtggct ttgagagaat gtttccaaat cactggaaga
    18361 gagcccttat tgcttcttgc tgatgaagtt tatcaagttg tcatgtctgt agaggaccag
    18421 gtggccagta gtttgaagga ctgaagtaaa tgccttctta aagtccaccc tgtagagcag
    18481 tggtgccaac cttcctaatg ctgtgaccct ttaatacagt tcctcatttt atgtgactcc
    18541 caaccataaa attatttttt attgctattc ataactgtaa ttttgctgct attaggaatt
    18601 gtaatgtaaa tatctgatac acaggatat
//