LOCUS       278188#pL1L2GT0_LF2A_nEGFPOT2A_CreERT2_pAbActNeopA#L3L4_pD223_DTA_T_spec        21874 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2GT0_LF2A_nEGFPOT2A_CreERT2_pAbActNeopA
COMMENT     design_id: 278188
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000022616
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            6919..7039
                     /db_xref="ENSEMBL:ENSMUSE00001206274"
                     /rank="2"
                     /note="ENSMUSE00001206274"
     exon            8512..8660
                     /db_xref="ENSEMBL:ENSMUSE00001233196"
                     /rank="3"
                     /note="ENSMUSE00001233196"
     misc_feature    4275..9580
                     /note="5 arm"
     misc_feature    9608..9632
                     /note="B1 Gateway"
     misc_feature    10599..10602
                     /note="FCHK QC critical region"
     misc_feature    10583..10598
                     /note="FCHK QC prefix region"
     misc_feature    10603..10608
                     /note="FCHK QC suffix region"
     misc_feature    10731..10782
                     /note="F2A"
     misc_feature    10608..10730
                     /note="1Dleader"
     primer          10468..10489
                     /vntifkey="27"
                     /dnas_title="seq primer FCHK2"
                     /label=seq primer FCHK2
                     /note="seq primer FCHK2"
     exon            10568..10607
                     /vntifkey="61"
                     /dnas_title="mouse En2 exon"
                     /label=mouse En2 exon
                     /note="mouse En2 exon"
     misc_feature    11654..11654
                     /note=" A to G to kill RsRII site"
     misc_feature    11604..11657
                     /note="self-cleaving' 2A peptide"
     misc_feature    11658..12683
                     /note="Cre"
     misc_feature    10788..10877
                     /note="3X NLS"
     misc_feature    10878..11594
                     /note="EGFP"
     primer          complement(9761..9782)
                     /vntifkey="27"
                     /dnas_title="LRPCR LAR3_EN2"
                     /label=LRPCR LAR3_EN2
                     /note="LRPCR LAR3_EN2"
     intron          9693..10567
                     /vntifkey="21"
                     /dnas_title="mouse En2 intron"
                     /label=mouse En2 intron
                     /note="mouse En2 intron"
     primer          complement(9657..9680)
                     /vntifkey="27"
                     /dnas_title="FRT3_oligo"
                     /label=FRT3_oligo
                     /note="FRT3"
     misc_feature    9645..9692
                     /vntifkey="21"
                     /dnas_title="Frt"
                     /label=Frt
                     /note="Frt"
     misc_feature    13944..13951
                     /vntifkey="21"
                     /dnas_title="polylinker"
                     /label=polylinker
     misc_recomb     13912..13943
                     /note="Rox(inv)"
     misc_feature    13904..13911
                     /vntifkey="21"
                     /dnas_title="RE sites"
                     /label=RE sites
     primer          13844..13862
                     /vntifkey="27"
                     /dnas_title="seq primer NF"
                     /label=seq primer NF
     primer          13718..13741
                     /vntifkey="27"
                     /dnas_title="LRPCR RAF5"
                     /label=LRPCR RAF5
     misc_feature    13661..13903
                     /vntifkey="21"
                     /dnas_title="SV40 pA"
                     /label=SV40 pA
     misc_feature    12693..13637
                     /note="ERT2"
     misc_feature    14739..15321
                     /note="synthetic fragment mutates RsRII"
     misc_feature    15151..15151
                     /note="G to C conservative mutation to kill RsrII site"
     misc_feature    15499..15742
                     /note="SV40pA"
     misc_feature    15557..15580
                     /note="RAF5"
     misc_feature    15306..15325
                     /note="neoBstB1_5"
     misc_feature    15013..15031
                     /note="neoSphI_5"
     misc_feature    14676..14695
                     /note="neoPstI_5"
     misc_feature    14507..15492
                     /note="neomycin resistance "
     misc_feature    14357..14376
                     /note="betact5"
     promoter        13961..14481
                     /note="human beta actin promoter"
     insert          13658..15737
                     /source="pL1L2_GTIRES_BetactP FLFL MapVerified"
     misc_feature    15738..15742
                     /note="for bActNeo"
     misc_recomb     15744..15775
                     /note="Rox"
     misc_feature    15788..15808
                     /note="B2 Gateway"
     misc_feature    9581..15838
                     /note="Synthetic Cassette"
     exon            16202..16372
                     /db_xref="ENSEMBL:ENSMUSE00001272117"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 1 ENSMUSE00001272117"
     exon            16940..17351
                     /db_xref="ENSEMBL:ENSMUSE00000497161"
                     /type="targeted"
                     /rank="5"
                     /note="target exon 2 ENSMUSE00000497161"
     misc_feature    15839..17566
                     /note="Critical Region"
     misc_feature    17590..17623
                     /note="Downstream LoxP"
     misc_feature    17567..17646
                     /note="synthetic loxP region"
     exon            18289..18393
                     /db_xref="ENSEMBL:ENSMUSE00000123109"
                     /rank="6"
                     /note="ENSMUSE00000123109"
     exon            21034..21263
                     /db_xref="ENSEMBL:ENSMUSE00000123110"
                     /rank="7"
                     /note="ENSMUSE00000123110"
     misc_feature    17647..21874
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggacctttc agttcctggg acagcgcata ataaataaga tgttgtgtat
     4321 ttaaagacgt gctgcagaca tccagacccg ttttgctgag ctgactgcat tcatccagtt
     4381 ctttagagaa atgctccacc aggccgagac gtctgatccc accggatcac tttatagatg
     4441 gtaatctgag gcctggagac cattagaggc tcagcctcca aacgttatat gaataagaaa
     4501 ctgaagccct ttggcttttc cagaatttct gggcccagtt ttaaaaatgg tgctggtgct
     4561 gtttttaaag gaagaagtta ggaattcaag agtctataca agttctctta acaggatcat
     4621 acaatttagt gcccgaccct gtgagaatag taaaacaaac tgcaaatggt agtcaatcaa
     4681 tggaatatta ggcagcagca aatgtgtgac ttttaaaaat atttaaaaac aggacctatg
     4741 aagggatggt ttataaaaat gcattggaaa aaaatggaga aaatacgcca caatgggaga
     4801 ggctatgttt tcctactaga taaggaggtc cttggccctg gtggggctga tgcacccagg
     4861 gtagttggca gccaggcagt aggccagcct ccaaaagaag acaggaagta agtcagtgta
     4921 gagggaaggc agcgggctga actgagcagc gttgtccttg gaacaacaac accacagcgc
     4981 cgggaaactt gggagactag cagattctga cccggagtcc atattaccag cagacctaga
     5041 gggagtctgt tcttagcttt actgaataga ggatcgcttc tttgaggtct gtatcaggtc
     5101 cattttacag ataaagaaac caaggccgag agatttagga ctttttattg tcaaggaaag
     5161 aatattaaaa tgaggtttca gagggctgca ttcttcctgg ggggtggaga gtgggggtgg
     5221 gggttggcta ctggcttgca gtgtgtcttg gggagcctgg caggtgtctg ggcgttggca
     5281 ttctctagaa gggcccaggc tgaagagcaa gcaaccctcc ctttcttatt tgggaacttg
     5341 gtggctctcc agaccccagg tctcctttgg caagccccaa gctccctgct ccctgtagct
     5401 catcttgata ctgcttcgcc tcccattcaa tggtcttcct tggctgccct gccctgctgc
     5461 ctcttcctgc ctcggatcag aagccatttc caaggctgtt aaatggcttt tcccaaaatg
     5521 gtcatgcttg aggctactcc cacctacaga tatttctgtg gctttgtctc tgatgtgtca
     5581 aggggtctgt ccctgcctgg agcatttcag gcctcagtga gcacctggga ggtctatgag
     5641 atcagggcct tcctagtgga tcatgaacca ggctggctga gccacctacc tatccctggg
     5701 aaagtctctt ctcccacacc actcctcctc tgctctgcgc gcgtaattaa gccctcagca
     5761 gaggccttgc ctattaatga tctgcccagt gtgttgatgg gcctgcctag ctgagagcag
     5821 gttttaagag tgagccattc caggaagaga gatcgctccc ttttccctgt tcctgtgggt
     5881 ataggaagtt ggtgagccca gataagctat tctgggcagc gttggagatg caaggggtgt
     5941 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgttt atgcaaccaa
     6001 tccagcttct gggaaaggat ccattgtgta atactggggc ttcagcctgt gtcccctggc
     6061 tcagggcagc cggcccagtg gtggcagtgg ccccttggca gagtatgcta gttcaggctt
     6121 agaggcaaca ggcatgtgaa aggaatttgc tggagccaat gggcttgtcc tcctgaaagg
     6181 gctttctggg gcggggggtg ggggtgggga tgggggctca ctgctaatct caggctttgt
     6241 ccttcccctt tctagttctg ccttctctgc agtcctaaat tccttccctt cccattttgc
     6301 ttttacccca ccccctcccc aatccatact cagcctggtg gcacaagcgt tgcccagaga
     6361 tggtgagatg gattgaattt cccgtgctga tggaagggtg tgcatattac cacaggctca
     6421 cttggatagc cttgggaggc tgagggcccc ctaaggggag cccagggctg tgcagtgagt
     6481 ggggatgcag taccatggaa ggggataggc gagggatgtt tgcatgtttc gggagaggta
     6541 tctttagtat ttggcatgtt aaatatgctc tggagacaca ggaaattcat ctgccctgcc
     6601 tcaggctctt atgggaagaa agggactcag cttggagctc ggcccctcca actccagata
     6661 gcctggctcc tctgtgcagc cgggccatgg ttcttcccag ggcctcgctg tctccagtgt
     6721 ctatagaagg ggtcagttta atcttggctt tcttccccag ttcttgagaa gtagattgca
     6781 gtgatgccag atgaggagac agatgagggc cgcgtgttcc aatcccaccc cacctcccct
     6841 ctcagggagc tgtagcaggg tgcagaaaac cctgaagctg cccaggggtt gccatgacac
     6901 atctgctgtt gtttccagga ctccagattc caaggaggcc acgccatgaa gattctcctg
     6961 ctgtgcgtgg cactgctgct gatctgggac aatggcatgg tcctgggaga gcaggaggtc
     7021 tctgacaatg agctccaagg taggtaggct gagactgttc ctctggtgga tgctataggg
     7081 cccagggcag ggccattcct caaagctcag tccccaagtg ctgagggctg ttttcattct
     7141 acatcctggg tctgtccatg ccctctgccc tctctaggtc ctgtcttgat gtttttgtgg
     7201 tcaggtgact tctagacatt gccctctgtc tcaagcccct ctgttggctg cctgtcattc
     7261 ctttgtcatt actggtcccc tcacctggct ctcttactct tctcccaaag ccgtgcccta
     7321 gaccctttga aactttgtca gtcttccctt tgtgtgacat ccagggttct taccgatgtg
     7381 tctccacacc aacccctcct ggaaagccat atccaccaat ttcctcctct ggaaaaaggg
     7441 caatgcaatt tgacatgaag tagtcacctg agagcaccct ctcccttcct ccccatcttc
     7501 caaggctttt ctggaaaccc accttttctc ccttcttctc tcctaacctc ttgctcatag
     7561 ctgaccagtt ctgggggggg gggtcttcgt taaagacaat gtgatcagat tctttgatgt
     7621 ggatacacac ggtgaaatga ttgctgttgt caagctatta gctgtccatc acctcaactt
     7681 atcattgggt tcagacactt aaaatatgtt ctctcagcac acttcaaggg tagaacacat
     7741 tatcaaccac gagctctgct tggaccattg accttcgggc ttacttgtgt tgctacatca
     7801 ttgaagctct tgcccttggc tcagcagtcc cccctcccgc ttctctttgg atgtgtctgg
     7861 caaccgcatc aggtagaaaa cagcagaggt gtgggaaatg gcacttgaca caggataagt
     7921 aagtggtacc accgtgacat gatgtgtcat tgccttcgtc acctggggcc gtgtcatggc
     7981 tatggtctcc atggtgaatg cagctcttca tttatcgtcc cgtctccatg gtgtctggtg
     8041 ctatcctgtc acctgaccct gtgcgttgta gcaaagaagt gccaggattg ggttaacctg
     8101 agcttcacag agataatttc caaaagagca cttttattac accttgatgc ttaggaggtt
     8161 gttttgtttt gttttctgtt ttccttgaaa tattctctgt ggctgttggc tctccagggt
     8221 ggcatggtgg ggtcagtcat agtatggctg cccctctctg caccactcac tcctgagttt
     8281 ctgagctgtg acttggagtc taccatgagc cctttgatgg gacatagagg caggactgag
     8341 gccataagga ctgccacagg cttggggtaa tagtgatggt gtctcagctt gacgccccca
     8401 gcactcccca tcaaagcatg gctttctcac cgtccccctg gcacccaggc tatttctcca
     8461 gccattcctc cccgcccccc tcaactcact gtaccttcct ctctctcaca gaactgtcca
     8521 ctcaagggag taggtatatt aataaggaga ttcagaacgc cgtccaggga gtgaagcaca
     8581 taaaaactct catagaaaaa accaacgcag agcgcaagtc cttgctcaac agtttagagg
     8641 aagccaagaa gaagaaagag gtagggccaa gccttggctg agcccaggct ctccctgctg
     8701 gaggggagga ggggacatgg tgagcaggtg cctttctggg ttgccatggt gtcctaaggc
     8761 ggtcctcgaa gggttccgct aatgctggac tgaggagcca gggaaagcac attcgcttgt
     8821 gggagcctca gtgccgaggc cttgaatttg gtttccctgc aactctggtc cagggcctga
     8881 agcctagatt catttcaata ggtttttcta acccttgctg tgggtagggc atggtgcagg
     8941 actcagcggt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgatatg agcatccaga
     9001 catttcccat tcttttcttc tgtattcaaa atgactttta acatgaaagt tcttattgaa
     9061 atattttaaa atcaatgtaa aattcttgta atctatcctt agacatccat ccattcattc
     9121 attcccactc agatataggg gaggggggca gttttcaaaa cccttagtag aagctgcagt
     9181 tgtggctagc accaggccct gcagagattg tgcttctgta atttataaat tacagacagt
     9241 aaggtatgag cagccacaat aaaatagagc aattataatg ctataccagt tataccatac
     9301 agaaggctac gcagatgtga tttctcccac acaaaatatc tcaattcctc agcttccaca
     9361 atactatttt tagactggtt gaccatggat agctaagaac tgataacaag ggactgccct
     9421 gttaccattt gtttaatttg tcatctacat ttgaacatca atgttgactc tggtacacac
     9481 acacacacac acacacacac acacacacac acacacacac aaacttatcc acagattcat
     9541 ccatccactt aattattttc catattgata tgtagacaca aaggcgcata acgataccac
     9601 gatatcaaca agtttgtaca aaaaagcagg ctggcgccgg aaccgaagtt cctattccga
     9661 agttcctatt ctctagaaag tataggaact tcgaaccctt tcccacacca ccctccacac
     9721 ttgccccaaa cactgccaac tatgtaggag gaaggggttg ggactaacag aagaacccgt
     9781 tgtggggaag ctgttgggag ggtcacttta tgttcttgcc caaggtcagt tgggtggcct
     9841 gcttctgatg aggtggtccc aaggtctggg gtagaaggtg agagggacag gccaccaagg
     9901 tcagcccccc ccccctatcc cataggagcc aggtccctct cctggacagg aagactgaag
     9961 gggagatgcc agagactcag tgaagcctgg ggtaccctat tggagtcctt caaggaaaca
    10021 aacttggcct caccaggcct cagccttggc tcctcctggg aactctactg cccttgggat
    10081 ccccttgtag ttgtgggtta cataggaagg gggacgggat tccccttgac tggctagcct
    10141 actcttttct tcagtcttct ccatctcctc tcacctgtct ctcgaccctt tccctaggat
    10201 agacttggaa aaagataagg ggagaaaaca aatgcaaacg aggccagaaa gattttggct
    10261 gggcattcct tccgctagct tttattggga tcccctagtt tgtgataggc cttttagcta
    10321 catctgccaa tccatctcat tttcacacac acacacacca ctttccttct ggtcagtggg
    10381 cacatgtcca gcctcaagtt tatatcacca cccccaatgc ccaacacttg tatggccttg
    10441 ggcgggtcat cccccccccc acccccagta tctgcaacct caagctagct tgggtgcgtt
    10501 ggttgtggat aagtagctag actccagcaa ccagtaacct ctgccctttc tcctccatga
    10561 caaccaggct cccaggtccc gaaaaccaaa gaagaagaac gcagatctct agagtcaccg
    10621 agttgcttta ccggatgaag agggccgaaa catactgtcc aaggcccttg ctggcaatcc
    10681 acccaactga agccagacac aaacagaaaa ttgtggcacc ggtgaaacag actttgaatt
    10741 ttgaccttct caagttggcg ggagacgtcg agtccaaccc cggaccggtg catgtggacc
    10801 ccaagaaaaa gcggaaggtg gaccctaaaa agaaacgcaa agtggatcct aagaaaaaaa
    10861 gaaaggtgca cgtgtccatg gtgtccaagg gcgaggaact gttcaccggc gtggtgccca
    10921 tcctggtgga actggatggc gacgtgaacg gccacaagtt cagcgtgtcc ggcgagggcg
    10981 aaggcgacgc cacatacgga aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
    11041 ccgtgccttg gcctaccctc gtgaccacac tgacctacgg cgtgcagtgc ttcagcagat
    11101 accccgacca tatgaagcag cacgacttct tcaagagcgc catgcccgag ggctacgtgc
    11161 aggaaagaac catcttcttt aaggacgacg gcaactacaa gaccagggcc gaagtgaagt
    11221 tcgagggcga caccctcgtg aacagaatcg agctgaaggg catcgacttc aaagaggacg
    11281 gcaacatcct gggccacaag ctggagtaca actacaacag ccacaacgtg tacatcatgg
    11341 ccgacaagca gaaaaacggc atcaaagtga acttcaagat ccggcacaac atcgaggacg
    11401 gctccgtgca gctggccgac cactaccagc agaacacccc tatcggcgac ggccctgtgc
    11461 tgctgcctga caaccactac ctgagcaccc agagcgccct gagcaaggac cccaacgaga
    11521 agagggacca catggtgctg ctggaattcg tgaccgccgc tggcatcacc ctgggcatgg
    11581 acgagctgta caagcctgca ggcgagggca gaggaagtct tctaacatgc ggtgacgtgg
    11641 aggagaatcc cgggccgtcc aatttactga ccgtacacca aaatttgcct gcattaccgg
    11701 tcgatgcaac gagtgatgag gttcgcaaga acctgatgga catgttcagg gatcgccagg
    11761 cgttttctga gcatacctgg aaaatgcttc tgtccgtttg ccggtcgtgg gcggcatggt
    11821 gcaagttgaa taaccggaaa tggtttcccg cagaacctga agatgttcgc gattatcttc
    11881 tatatcttca ggcgcgcggt ctggcagtaa aaactatcca gcaacatttg ggccagctaa
    11941 acatgcttca tcgtcggtct gggctgccac gaccaagtga cagcaatgct gtttcactgg
    12001 ttatgcggcg gatccgaaaa gaaaacgttg atgccggtga acgtgcaaaa caggctctag
    12061 cgttcgaacg cactgatttc gaccaggttc gttcactcat ggaaaatagc gaccgctgcc
    12121 aggatataag gaatctggca tttctgggga ttgcttataa caccctgtta cgtatagccg
    12181 aaattgccag gatcagggtt aaagatatct cacgtactga cggtgggaga atgttaatcc
    12241 atattggcag aacgaaaacg ctggttagca ccgcaggtgt agagaaggca cttagcctgg
    12301 gggtaactaa actggtcgag cgatggattt ccgtctctgg tgtagctgat gatccgaata
    12361 actacctgtt ttgccgggtc agaaaaaatg gtgttgccgc gccatctgcc accagccagc
    12421 tatcaactcg cgccctggaa gggatttttg aagcaactca tcgattgatt tacggcgcta
    12481 aggatgactc tggtcagaga tacctggcct ggtctggaca cagtgcccgt gtcggagccg
    12541 cgcgagatat ggcccgcgct ggagtttcaa taccggagat catgcaagct ggtggctgga
    12601 ccaatgtaaa tattgtcatg aactatatcc gtaacctgga tagtgaaaca ggggcaatgg
    12661 tgcgcctgct ggaagatggc gatctcgagc catctgctgg agacatgaga gctgccaacc
    12721 tttggccaag cccgctcatg atcaaacgct ctaagaagaa cagcctggcc ttgtccctga
    12781 cggccgacca gatggtcagt gccttgttgg atgctgagcc ccccatactc tattccgagt
    12841 atgatcctac cagacccttc agtgaagctt cgatgatggg cttactgacc aacctggcag
    12901 acagggagct ggttcacatg atcaactggg cgaagagggt gccaggcttt gtggatttga
    12961 ccctccatga tcaggtccac cttctagaat gtgcctggct agagatcctg atgattggtc
    13021 tcgtctggcg ctccatggag cacccagtga agctactgtt tgctcctaac ttgctcttgg
    13081 acaggaacca gggaaaatgt gtagagggca tggtggagat cttcgacatg ctgctggcta
    13141 catcatctcg gttccgcatg atgaatctgc agggagagga gtttgtgtgc ctcaaatcta
    13201 ttattttgct taattctgga gtgtacacat ttctgtccag caccctgaag tctctggaag
    13261 agaaggacca tatccaccga gtcctggaca agatcacaga cactttgatc cacctgatgg
    13321 ccaaggcagg cctgaccctg cagcagcagc accagcggct ggcccagctc ctcctcatcc
    13381 tctcccacat caggcacatg agtaacaaag gcatggagca tctgtacagc atgaagtgca
    13441 agaacgtggt gcccctctat gacctgctgc tggaggcggc ggacgcccac cgcctacatg
    13501 cgcccactag ccgtggaggg gcatccgtgg aggagacgga ccaaagccac ttggccactg
    13561 cgggctctac ttcatcgcat tccttgcaaa agtattacat cacgggggag gcagagggtt
    13621 tccctgccac agcttgatga agatctgagc tcttaattaa tgatcataat cagccatatc
    13681 acatctgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
    13741 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
    13801 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
    13861 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccgtttaaa ctaactttaa
    13921 ataattggca ttatttaaag ttacatatgg cggccaattc gccttctgca ggagcgtaca
    13981 gaacccaggg ccctggcacc cgtgcagacc ctggcccacc ccacctgggc gctcagtgcc
    14041 caagagatgt ccacacctag gatgtcccgc ggtgggtggg gggcccgaga gacgggcagg
    14101 ccgggggcag gcctggccat gcggggccga accgggcact gcccagcgtg ggcgcggggg
    14161 ccacggcgcg cgcccccagc ccccgggccc agcaccccaa ggcggccaac gccaaaactc
    14221 tccctcctcc tcttcctcaa tctcgctctc gctctttttt tttttcgcaa aaggagggga
    14281 gagggggtaa aaaaatgctg cactgtgcgg cgaagccggt gagtgagcgg cgcggggcca
    14341 atcagcgtgc gccgttccga aagttgcctt ttatggctcg agcggccgcg gcggcgccct
    14401 ataaaaccca gcggcgcgac gcgccaccac cgccgagacc gcgtccgccc cgcgagcaca
    14461 gagcctcgcc tttgccgatc ctctagagtc gagatccgcc gccaccatga ttgaacaaga
    14521 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
    14581 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
    14641 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc
    14701 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
    14761 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
    14821 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
    14881 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
    14941 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
    15001 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt
    15061 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
    15121 attcatcgac tgtggccggc tgggtgtggc cgaccgctat caggacatag cgttggctac
    15181 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
    15241 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
    15301 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat
    15361 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
    15421 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccccggat
    15481 ctaagctcta gataagtaat gatcataatc agccatatca catctgtaga ggttttactt
    15541 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
    15601 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
    15661 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
    15721 gtatcttatc atgtctggat ccctaacttt aaataatgcc aattatttaa agttaggaat
    15781 taattcaccc agctttcttg tacaaagtgg ttgatatctc tatagtcgca gtaggcgggg
    15841 catggtaatg agtttgatgg ttgatttgac tggatagctt cgatttgcat gtcagaggcc
    15901 acagttctaa cagaaagata agactgtcct ctttggggag atgagaggag acagtggggt
    15961 ggtttaaagg agaggtgggg cctaaggaag agacggtcag agcagcagat cagagtggac
    16021 agggaagctt agggaatggc tgggccttga agaaggagag gctcctgaga gcgtggctgg
    16081 ggaggcccaa cagaagggct agagcgaagc tgtgggcacc aggagcaacc ttgtactcag
    16141 atcggggaga ccggctgggt ctctgtgtgg gtcacctggc cttcttgtca tctccctgaa
    16201 ggatgctctg gaggacacta gggattctga aatgaagctg aaggcattcc cggaagtgtg
    16261 taacgagacc atgatggccc tctgggagga gtgcaagccc tgcctgaagc atacctgcat
    16321 gaagttctat gcacgtgtct gcaggagcgg ctcagggctg gtcggccagc aggtgagaag
    16381 gagagatgtc cacagcctgg gttctggatg gggaatgtgg agaatccatg aaataggcct
    16441 cattccatat gctgggtaca gttgattagc attgcttgac tgcacatcgt tctagtgtca
    16501 tggtcgattg gtgatgtctg cttggcaggt accatctctt tcaagacctt taactggttg
    16561 ggtaaaatgg ccttatggga tggtagaatc ctgtgttaga ataggaaaag ggaattaaaa
    16621 aatatttagt ctatttgcat taattctatc cctacctgtg agattcactt ggttggaact
    16681 cagtaaaatt cactgtttcc tcaaggctga gcagctaagc aggagtggct atttattctt
    16741 tatggcccaa ggtgagactt gggaacctcc gagtttcgcc atgagaaatg gatttggggg
    16801 gcggcggcgg ggggacgggg ggtgctgaca gggtacccag gaggcagaac agtgggaact
    16861 tgggtcagtt tctgtgtggc cacaggggga tacaggctgg agtacacaca atgccttact
    16921 ctacttctct gggtcccagc tagaggagtt tctaaaccag agctcaccct tctacttctg
    16981 gatgaacggc gaccgcatcg actccctgct ggagagtgac cggcagcaga gccaagtcct
    17041 ggatgccatg caggacagct ttgctcgggc atctggcatc atagacacgc tctttcagga
    17101 ccggttcttc gcccgtgagc ttcatgaccc ccactacttc tcccccatcg gcttcccaca
    17161 caagaggcct catttcttat atcccaagtc ccgcttggtc cgcagcctca tgtctccctc
    17221 ccactatggg cctccgagct tccacaacat gttccagcct ttctttgaga tgatccacca
    17281 ggctcaacag gccatggatg tccagctcca cagcccagcc ttccagttcc cagacgtgga
    17341 tttcttaaga ggttagaaag caattgcttt ctttctaagt aggaatgtga atgtcccaga
    17401 tgtctaggct cttccagggc ttgacctgtg ctggttttat gttcctagaa gcctcagagg
    17461 ctggtatttc ctccatttgt agggtaaagg agctaggatg ggggcaaaga aatgcaggta
    17521 aatgtggggg ggcgcagttc aattagcatt tgtagagatt ggagctgaga tggcgcaacg
    17581 caattaatga taacttcgta tagcatacat tatacgaagt tatggtctga gctcgccatc
    17641 agttcatccc agatattatt tcttgggaac ttttgagggt gtggtgtgag aggcccgccc
    17701 ttccaggcaa tgagtctgtg tcatcttaaa gaagatgttc atggctgtga ccttagcgtg
    17761 taatgcctgt aaggatgaaa tatcacgtgg acacgagaaa ccggtgtctg aggagtgatt
    17821 ttttttttcc attagtgcct tgggtcttaa taccatgtcc tacatggtat ggtttgcatt
    17881 agaaatagaa gaagtcttgg catccaacac acatctactg tagctccatt gtctaaagat
    17941 ggtttatgtt gtcaggacac acttgtgctc tctggggctt gaaggccagt tagggagtac
    18001 catcagtcaa ggagaacaaa gaacagccat gatgtcagaa ggcacacagc tgaggggatg
    18061 tggcaggaga gatctcctca gatggagatg gtgggaaggc aggcaggacc tgctgagagc
    18121 cgtgggaata tcctcagcca cagggaagtc ccatggctgc aagcaaacaa ataagctgca
    18181 tcgtgccctg accatggcac tgagcagagg tgtggaaggc tgtcgggtgg gcatccttct
    18241 gatgtgccat gtgtctaatc ctgcccctct ttcccctcat gtgtccagaa ggtgaagatg
    18301 accgcactgt gtgcaaggag atccgccgca actccacagg atgcctgaag atgaagggcc
    18361 agtgtgaaaa gtgccaggag atcttgtctg tgggtgagtc cgggaaccag tggtcatctg
    18421 actgtgggtc tccagtgact cactggcact atccttttcc aaagactagc tctctgcctg
    18481 ggaactctta atccccggga gactcctcac cctggtgcac agcctactct cctcttatct
    18541 agagcttcat tatttgggag ctggggtagg acccagtgcc tcctgcatgt taggtgaacg
    18601 ctctaccact gagccacatc aattttactc agaatcaagt tcaggtgcag attgttctca
    18661 tatagatgag gtggagctaa agtgcccacc accacccctg tggctcactg gcaaacctgc
    18721 agtccacggt ggaattacac tgtatcgacc agtaagccac agctcgttcc cctggctact
    18781 ttgttccaca gtgccaagta ctgtagaccc tgattttgaa cttctgatta tgaaatcttt
    18841 gacaacatgc gttttgttta acaagtgctc gccacatgcc aggcactgct gcaaacacgt
    18901 cacaggtgtc tgttcgcaga atgatcctgt gagctgggta cagctctctt tccgtcttgc
    18961 tggatggcta agtgacttat ctaagattac gtgacacgag gcttctcact taggctgtgc
    19021 tcataaacag caccctgggg tttctccctg gggaccttga gctatgtcct tgtgatggtg
    19081 gtccttggta tcctggccgc attctttttg gtgaactagt ccctcagaaa aggaactatt
    19141 tttccttcat cagttcccca ggcaatctgg gtatggtgac atatccctat aatcccaaca
    19201 tttgggaggc tgaggtagga gggtggtgaa tccgaggcca gcggcgaggt tctgtctcaa
    19261 tgaaaacaaa acaaaataca gcaaacccaa aactgttccc cacagatttg tcaggtctgg
    19321 ctatcaagcc aatgccccgc cttcagcctg gtctttacag tggctcacag tacacggtgc
    19381 tacctgctca gatcaccctc tttgttggcc gggacagacc tttagagtct ggttaaatat
    19441 tcccagcctc attcctgcaa tgttaggagg ctaggaaaaa aaaaaaaata ctagtgcttt
    19501 caaaggtcat agtgtgacat tataaacaaa tatgggccac acctgtaggc tttcccttat
    19561 agttgaaaga tggggtgcct gagcacagag aagccagtca ccttatagac tggatctctt
    19621 acatcagact tagcgatcct ctcaacagtc acccgttagc tcttatttac ggtgcttata
    19681 atggtatcct ctcagcagtc accgtttctg tcacccgtta gctcttatgt acagtgctta
    19741 caatggtatg gataggcttc acagagacct ctcgcctggg acatcttgcc tcagccctgt
    19801 gggcagacct gaccatggct gagcatttaa caacattttt ggctccaacc taccatctac
    19861 caagagcaac cctccctcca ctgtgacaat cagaaacatc ttcagattgt ctggactgtc
    19921 tcctggtggg tggtgtggtc ccaggttgag aaccaccgtt ctactctgtc cttctctcag
    19981 gactttcttg tttgggctaa tcagtggtta gagtgctccg gttattcctg gaagatttct
    20041 tctcatttct gcttctggtc tccaatcctg tgtcttgccc catgcaacat tatatcttat
    20101 tttaagtatg aacaattgta gtagccacat agggcccaga aggcatctct cttgtcttgc
    20161 ttagtttgga tatagcctct catagatgtt taaaaaataa ttgaggaggg gggaggcgtt
    20221 gttaaacact ggttttcaga aagcatgagc actttgttgg tctcagtagc ctgatgcctg
    20281 cagtgcactc ttgcagaaga gaggatgcca gagttcctat ttcctcctgg tcatcctggg
    20341 tggacaaggg caggcaagga ctgacccttg ggcaccaaac acctgtgttt tgtagactcg
    20401 ggacctggct gtctcttctg caggtttggt tctgggaaaa tggtcttgcc gaatctgtca
    20461 aattatgttc aaaaataagt ttggagaata ttctagaacc ctggtctcag gggctcaggt
    20521 tgaggagtga aagatggggc ttcaatcctg gttccacaga tcgcttcctc tatagatctg
    20581 agcaaggtcc acctaagtaa ataatgatcc tgttctttgt ggggtgtcca gtgccacagt
    20641 agcttcctcc tattttggaa atgagtcctg ggaggagctc agggagcaag agtaagtcca
    20701 ggaacatggg gtagtcagca taggtgctcc ccagagcacc gtcagcagca ttgtgacagg
    20761 tcaccaagac tggtcatgtc taccgcccaa agaagactca gccacgctgc tctgacacag
    20821 tttagcacag ctaagccatt gtatcccaaa gcacgaaggg ggcagcacct tggtttccgt
    20881 gtccggggaa ctgtaagagc tgatctgtag ccctccctgg aagagttcct ctgcttcaga
    20941 ttgtggctgc tgcagctggt tcccaggggc gtgtccgtcc cctttaccct ccctttcatg
    21001 cttctgcctt ctgcttcttt cttctctggg cagactgttc aaccaacaat cctgcccagg
    21061 ctaacctgcg ccaggagctg aacgactcgc tccaggtggc cgagaggctg acagagcagt
    21121 acaaggagct gctgcagtcc ttccagtcga agatgctcaa cacctcatcc ctgctggagc
    21181 agctgaacga ccagttcaac tgggtgtccc agctggctaa cctcacacag ggcgaagaca
    21241 agtactacct tcgggtctcc accgtgagtt gtatcccagt catttgatga ggctgagacc
    21301 cgggtgtgat ccagaggtga tgtgtgtctc ttagagatgt ggtgggacaa aggtcccacc
    21361 taggatttac accagcaggg actaggaaaa ccgtatgcac gttgtagcat tgaaatgcct
    21421 ttgccccgtc aaatatccaa gtctcattgt caagatgagg aaagagagag gttctgtggt
    21481 ttgctaagaa cagacagttt tgggcaactt ccctgggtca gcctgtcagc ctggctgtaa
    21541 agctgcttcc tgcccagggc cactctgagt gccacatgaa ttggttcact gttagccagt
    21601 gagaactcgg tttcctttag tttgtggacc ccacccctac cccctttgac cacagactgg
    21661 ctaacatttg tacaaagcaa atgttggggc caggtgttgt ggtgcgtgcc tgtaagccag
    21721 cactgaagaa actgaggcag gagagtagag agcttgagcc tgggctggac tatatagtga
    21781 cagcctctct caaacaaagc aaaacaagag aacacaacaa gaagaaaaca ttcaaaaatc
    21841 atttcaagtt tctggagtgc ctacttggct agtc
//