LOCUS       50415#pL1L2GT1_LF2A_nEGFPOT2A_CreERT2_pAbActNeopA#L3L4_pD223_DTA_T_spec        20966 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2GT1_LF2A_nEGFPOT2A_CreERT2_pAbActNeopA
COMMENT     design_id: 50415
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000034756
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     misc_feature    4275..8231
                     /note="5 arm"
     misc_feature    8259..8283
                     /note="B1 Gateway"
     misc_feature    9250..9267
                     /note="FCHK QC critical region"
     misc_feature    9234..9249
                     /note="FCHK QC prefix region"
     misc_feature    9268..9273
                     /note="FCHK QC suffix region"
     misc_feature    9396..9447
                     /note="F2A"
     misc_feature    9273..9395
                     /note="1Dleader"
     primer          9119..9140
                     /vntifkey="27"
                     /dnas_title="seq primer FCHK2"
                     /label=seq primer FCHK2
                     /note="seq primer FCHK2"
     exon            9219..9272
                     /vntifkey="61"
                     /dnas_title="mouse En2 exon"
                     /label=mouse En2 exon
                     /note="mouse En2 exon"
     misc_feature    10319..10319
                     /note=" A to G to kill RsRII site"
     misc_feature    10269..10322
                     /note="self-cleaving' 2A peptide"
     misc_feature    10323..11348
                     /note="Cre"
     misc_feature    9453..9542
                     /note="3X NLS"
     misc_feature    9543..10259
                     /note="EGFP"
     primer          complement(8412..8433)
                     /vntifkey="27"
                     /dnas_title="LRPCR LAR3_EN2"
                     /label=LRPCR LAR3_EN2
                     /note="LRPCR LAR3_EN2"
     intron          8344..9218
                     /vntifkey="21"
                     /dnas_title="mouse En2 intron"
                     /label=mouse En2 intron
                     /note="mouse En2 intron"
     primer          complement(8308..8331)
                     /vntifkey="27"
                     /dnas_title="FRT3_oligo"
                     /label=FRT3_oligo
                     /note="FRT3"
     misc_feature    8296..8343
                     /vntifkey="21"
                     /dnas_title="Frt"
                     /label=Frt
                     /note="Frt"
     misc_feature    12609..12616
                     /vntifkey="21"
                     /dnas_title="polylinker"
                     /label=polylinker
     misc_recomb     12577..12608
                     /note="Rox(inv)"
     misc_feature    12569..12576
                     /vntifkey="21"
                     /dnas_title="RE sites"
                     /label=RE sites
     primer          12509..12527
                     /vntifkey="27"
                     /dnas_title="seq primer NF"
                     /label=seq primer NF
     primer          12383..12406
                     /vntifkey="27"
                     /dnas_title="LRPCR RAF5"
                     /label=LRPCR RAF5
     misc_feature    12326..12568
                     /vntifkey="21"
                     /dnas_title="SV40 pA"
                     /label=SV40 pA
     misc_feature    11358..12302
                     /note="ERT2"
     misc_feature    13816..13816
                     /note="G to C conservative mutation to kill RsrII site"
     misc_feature    14164..14407
                     /note="SV40pA"
     misc_feature    14222..14245
                     /note="RAF5"
     misc_feature    13971..13990
                     /note="neoBstB1_5"
     misc_feature    13678..13696
                     /note="neoSphI_5"
     misc_feature    13341..13360
                     /note="neoPstI_5"
     misc_feature    13172..14157
                     /note="neomycin resistance "
     misc_feature    13022..13041
                     /note="betact5"
     promoter        12626..13146
                     /note="human beta actin promoter"
     insert          12323..14402
                     /source="pL1L2_GTIRES_BetactP FLFL MapVerified"
     misc_feature    14403..14407
                     /note="for bActNeo"
     misc_recomb     14409..14440
                     /note="Rox"
     misc_feature    14453..14473
                     /note="B2 Gateway"
     misc_feature    8232..14503
                     /note="Synthetic Cassette"
     exon            15753..15847
                     /db_xref="ENSEMBL:ENSMUSE00000217973"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 1 ENSMUSE00000217973"
     misc_feature    14504..16017
                     /note="Critical Region"
     misc_feature    16041..16074
                     /note="Downstream LoxP"
     misc_feature    16018..16097
                     /note="synthetic loxP region"
     exon            19354..19467
                     /db_xref="ENSEMBL:ENSMUSE00000217975"
                     /rank="5"
                     /note="ENSMUSE00000217975"
     misc_feature    16098..20966
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggattttaa aatagaacca cctaagattc ccagcaaggc tacaagacca
     4321 ctctcctcct atgcagcgga gaagggcaga acgtctttct tgtaagattg gggtgcctct
     4381 gtgggctgca ccccataaag aagtccttca taactcattg gcccagggcc atattgttcg
     4441 actagaaggg aaactggccc cactcttccc tttgcagagg cctctcgctt gaggctccgg
     4501 gtcactttgc aaaccagccc tcatctgaca gctgttctca gagtcagacc ctgttgagcc
     4561 ttcacagctg ctttaactcc tgagcacagc attcctgtct aagagcattg tctgcatagc
     4621 tgtgcaggaa ccaggtggct gcaaagcact ggggagggag tgcccagggc tctttcattg
     4681 ctggcttcat tggctgcctc tccctcctca gccctttctt ttttctactg aggggatcca
     4741 aaaaccagtt tcacccacga acgttggaag aaaccagatt ctaatctggc atctgcacgg
     4801 tgggctgagt gaagggtgag cctgcagggt tccgttgttg cagctgttca ggtttttcct
     4861 ttcctgttct ctgaggcaac tagggtacca gggcataaaa aggtgcccac attgtacaca
     4921 tgttactcca tttttgtttt tttgtttttt tgtttttatt tctttgtgtt ttcctactgg
     4981 ttctaggtgg aattttagaa acacactaga ttattctctg agaattagaa gggctttttt
     5041 ttttttttta attattttta agtcagggtc tcttgtattg cagaacttgc catgtagcca
     5101 cagatgactt taagtttcta atcctctggt tcccacctcc caagtgctag gattacaagg
     5161 atgtgccatg acacccagtt tatgtggcac caggttccaa actcaggact tacatgctag
     5221 acaaccattc tacctactga gttatacccc caagcctggg tttttagatg acaaatgcat
     5281 cccataccga taacaaagga gactaggcct taggtcctat ctaagaaacc tagccgaggt
     5341 gacatcagaa cagatgggaa tagaagtttg atctttgggc taaagtgctt tcttgggtat
     5401 tccgttgagc taggccatcc ttgaactggg tctgtgtccc tcaactatag cacacctgtc
     5461 agaggcctga tgctcctcag cccctcccct gggtatccaa ttcttgtctc agctttcagg
     5521 agtttctgtt gttcacctgg aaactgcact gttaacctca tgaaagctat tagcaaacac
     5581 ggcctgggct tctctctgcc ctgtcttttc ataggccaat agttgaatcc aggcagtggc
     5641 tgggtctggg gctgagaggc caagggaggg acttgcctcc catcattcag tcagtctttt
     5701 ttgtgtccta gcaacataag tcttacgatg gttcctgttt tatatctggg aaagccaggt
     5761 gcagagagag aagcattgaa ccccaaggtc gtatatgctt gagtctcagg tacccttcag
     5821 aatatcgcct cctctgctta cacagagacc tcctgtattc ggcactgtct catcccgggg
     5881 ccaaaatgcc aaacaaaagc gagttaaggg agagagggtt tactctggct catagtcgga
     5941 tagcccagcc atagtgcacc aggcatggag cagagcgagg aatactggtg ctcccctcac
     6001 agtgctccat tttccgtagt ccaggacctc cgccaatggg atggtgctgt ccatatttac
     6061 agtgggcttt cccacctcag tcaaccctac ctaggtaacc cttcactagt catgcataga
     6121 agtttgtctc ctaggtgatt tcagatcctg tcaagtcagt aataacaact atcacagtat
     6181 ccctgactcc ctggcccggt cctctctggc atactttagg cccttttgtg aagacagagg
     6241 atttcttcta agccaaagat atcaaagata gccagcaatc tgagctgtag tcctatcctc
     6301 ccgcccccaa ctagataagg caattttcct tttctttcca accagacagc tcccaccccg
     6361 aggcctcttg agccctcatc agacagacag aatctactct atgtgcagtc agcatgtctc
     6421 tggtgttgtg aatcagcatc ctgttccaga gggttcttgg ttccctgcca gagacagtgg
     6481 agacacgcat tctccatgga atgccgaagt ccagagcaga ccaaggattt cagcccgcag
     6541 agaggaagag agcaggggcg ggctttgtgg gtttggattc aatgcttgaa accttttaat
     6601 gcttgaaaaa tatggtgggc agctgagaag actcagcccc tcaaagtcac tgctggaact
     6661 cagctgtcag ttatgcaaag acctccctca ggtagactct gcttagaaca atgatggacc
     6721 tttttttttt ttaaccatgt cttcaattgg aatattgaaa atatgcaggt gattagacta
     6781 gccaaccatc ttcatccaag gacaacatgc ttaactgcga ccctgcaccc gtgtgtcact
     6841 acatcttggg tcttagttgg gcttaggcca cttgacttct gtaaaatttg agtctgttgg
     6901 ataagttttt acaaagatca cctgagtggg gcccagcagg taagaatgtt tgctgctctc
     6961 gcagaggact caagtttggt tcctagtacc caagttgagc tattcacagc cgcctgtaga
     7021 tgcagctgta ggggatctgt ggacgctttc gtctggccca cagtctcttt agatctcaga
     7081 tccttctacc tagccagtcc ttccttaaat ggggggaagt gagaagttgg aaagaaccca
     7141 gtgcacggat gcagagcctt acctgctaag cttctggagc taaccttgaa ggcatggccg
     7201 tcagtattga ttacaatgcc cacctggatg gctgagtgtt tgccccacct ccttcatctg
     7261 tagttagact gtggtggcca atggccgctt ggtccctgca gcttcaagct atgctggata
     7321 gttttccaca ggaacaaact gtttcatcca ctgggacatc aggtcaagga agcaatagtg
     7381 gtctaaagat aacatcaccg ggagagatgg ctcagaggtt aagagcactg tctcttcttc
     7441 caggggtccc aagttcaatt cccagcaact gcacggtggc tcacagccat ttgtaatgag
     7501 atctggtgtc ttctactgga ttgcagtcat acatgcaggc agaatgatgt atacataata
     7561 aataaatctt ttttttaaag ataatatcac caatgggtat tctggtgctt tagcataggt
     7621 tatgccttat gtgccagctc aaggcacata agcccagaga atgctgggct tcttgagaca
     7681 tgccagcaac tacctgtgac acagcactga tgcagatgag ggtttagagg gtttagggtc
     7741 cagagctgcc cacttgtaga ggatctctgt tcagtttctc tgcattgact gtcatcagca
     7801 tgatactgcg aaacctttga caaatctgct tctagatcca gccttctaca cagatccatc
     7861 catattcctt tgttggtttt gcttgtttgt ttttcgagac agggtttctt tgtatagtcc
     7921 atacagcttt aatctcagca ggcaggaggc aggaggcagg aggcaggagg caggaggcag
     7981 gaggcaggag gcaggaggca ggaggcagga ggcaggaggc aggaggcagg aggcaggagg
     8041 caggaggcag gaggcaggca gatttctgtg aatttgagac cagctgggtg aatccctgtc
     8101 tcaaaaccaa aaaccaccaa aaaactgaaa aaacaaacaa acaaaagcac aacaacaaca
     8161 aaaaatcctc aaaaaagaat aaaaataatt caccttgctt tattttaaat gaccacatac
     8221 atagcatagt aaaggcgcat aacgatacca cgatatcaac aagtttgtac aaaaaagcag
     8281 gctggcgccg gaaccgaagt tcctattccg aagttcctat tctctagaaa gtataggaac
     8341 ttcgaaccct ttcccacacc accctccaca cttgccccaa acactgccaa ctatgtagga
     8401 ggaaggggtt gggactaaca gaagaacccg ttgtggggaa gctgttggga gggtcacttt
     8461 atgttcttgc ccaaggtcag ttgggtggcc tgcttctgat gaggtggtcc caaggtctgg
     8521 ggtagaaggt gagagggaca ggccaccaag gtcagccccc cccccctatc ccataggagc
     8581 caggtccctc tcctggacag gaagactgaa ggggagatgc cagagactca gtgaagcctg
     8641 gggtacccta ttggagtcct tcaaggaaac aaacttggcc tcaccaggcc tcagccttgg
     8701 ctcctcctgg gaactctact gcccttggga tccccttgta gttgtgggtt acataggaag
     8761 ggggacggga ttccccttga ctggctagcc tactcttttc ttcagtcttc tccatctcct
     8821 ctcacctgtc tctcgaccct ttccctagga tagacttgga aaaagataag gggagaaaac
     8881 aaatgcaaac gaggccagaa agattttggc tgggcattcc ttccgctagc ttttattggg
     8941 atcccctagt ttgtgatagg ccttttagct acatctgcca atccatctca ttttcacaca
     9001 cacacacacc actttccttc tggtcagtgg gcacatgtcc agcctcaagt ttatatcacc
     9061 acccccaatg cccaacactt gtatggcctt gggcgggtca tccccccccc cacccccagt
     9121 atctgcaacc tcaagctagc ttgggtgcgt tggttgtgga taagtagcta gactccagca
     9181 accagtaacc tctgcccttt ctcctccatg acaaccaggc tcccaggtcc cgaaaaccaa
     9241 agaagaagaa cgcagatcgc agatcgcaga tctctagagt caccgagttg ctttaccgga
     9301 tgaagagggc cgaaacatac tgtccaaggc ccttgctggc aatccaccca actgaagcca
     9361 gacacaaaca gaaaattgtg gcaccggtga aacagacttt gaattttgac cttctcaagt
     9421 tggcgggaga cgtcgagtcc aaccccggac cggtgcatgt ggaccccaag aaaaagcgga
     9481 aggtggaccc taaaaagaaa cgcaaagtgg atcctaagaa aaaaagaaag gtgcacgtgt
     9541 ccatggtgtc caagggcgag gaactgttca ccggcgtggt gcccatcctg gtggaactgg
     9601 atggcgacgt gaacggccac aagttcagcg tgtccggcga gggcgaaggc gacgccacat
     9661 acggaaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccttggccta
     9721 ccctcgtgac cacactgacc tacggcgtgc agtgcttcag cagatacccc gaccatatga
     9781 agcagcacga cttcttcaag agcgccatgc ccgagggcta cgtgcaggaa agaaccatct
     9841 tctttaagga cgacggcaac tacaagacca gggccgaagt gaagttcgag ggcgacaccc
     9901 tcgtgaacag aatcgagctg aagggcatcg acttcaaaga ggacggcaac atcctgggcc
     9961 acaagctgga gtacaactac aacagccaca acgtgtacat catggccgac aagcagaaaa
    10021 acggcatcaa agtgaacttc aagatccggc acaacatcga ggacggctcc gtgcagctgg
    10081 ccgaccacta ccagcagaac acccctatcg gcgacggccc tgtgctgctg cctgacaacc
    10141 actacctgag cacccagagc gccctgagca aggaccccaa cgagaagagg gaccacatgg
    10201 tgctgctgga attcgtgacc gccgctggca tcaccctggg catggacgag ctgtacaagc
    10261 ctgcaggcga gggcagagga agtcttctaa catgcggtga cgtggaggag aatcccgggc
    10321 cgtccaattt actgaccgta caccaaaatt tgcctgcatt accggtcgat gcaacgagtg
    10381 atgaggttcg caagaacctg atggacatgt tcagggatcg ccaggcgttt tctgagcata
    10441 cctggaaaat gcttctgtcc gtttgccggt cgtgggcggc atggtgcaag ttgaataacc
    10501 ggaaatggtt tcccgcagaa cctgaagatg ttcgcgatta tcttctatat cttcaggcgc
    10561 gcggtctggc agtaaaaact atccagcaac atttgggcca gctaaacatg cttcatcgtc
    10621 ggtctgggct gccacgacca agtgacagca atgctgtttc actggttatg cggcggatcc
    10681 gaaaagaaaa cgttgatgcc ggtgaacgtg caaaacaggc tctagcgttc gaacgcactg
    10741 atttcgacca ggttcgttca ctcatggaaa atagcgaccg ctgccaggat ataaggaatc
    10801 tggcatttct ggggattgct tataacaccc tgttacgtat agccgaaatt gccaggatca
    10861 gggttaaaga tatctcacgt actgacggtg ggagaatgtt aatccatatt ggcagaacga
    10921 aaacgctggt tagcaccgca ggtgtagaga aggcacttag cctgggggta actaaactgg
    10981 tcgagcgatg gatttccgtc tctggtgtag ctgatgatcc gaataactac ctgttttgcc
    11041 gggtcagaaa aaatggtgtt gccgcgccat ctgccaccag ccagctatca actcgcgccc
    11101 tggaagggat ttttgaagca actcatcgat tgatttacgg cgctaaggat gactctggtc
    11161 agagatacct ggcctggtct ggacacagtg cccgtgtcgg agccgcgcga gatatggccc
    11221 gcgctggagt ttcaataccg gagatcatgc aagctggtgg ctggaccaat gtaaatattg
    11281 tcatgaacta tatccgtaac ctggatagtg aaacaggggc aatggtgcgc ctgctggaag
    11341 atggcgatct cgagccatct gctggagaca tgagagctgc caacctttgg ccaagcccgc
    11401 tcatgatcaa acgctctaag aagaacagcc tggccttgtc cctgacggcc gaccagatgg
    11461 tcagtgcctt gttggatgct gagcccccca tactctattc cgagtatgat cctaccagac
    11521 ccttcagtga agcttcgatg atgggcttac tgaccaacct ggcagacagg gagctggttc
    11581 acatgatcaa ctgggcgaag agggtgccag gctttgtgga tttgaccctc catgatcagg
    11641 tccaccttct agaatgtgcc tggctagaga tcctgatgat tggtctcgtc tggcgctcca
    11701 tggagcaccc agtgaagcta ctgtttgctc ctaacttgct cttggacagg aaccagggaa
    11761 aatgtgtaga gggcatggtg gagatcttcg acatgctgct ggctacatca tctcggttcc
    11821 gcatgatgaa tctgcaggga gaggagtttg tgtgcctcaa atctattatt ttgcttaatt
    11881 ctggagtgta cacatttctg tccagcaccc tgaagtctct ggaagagaag gaccatatcc
    11941 accgagtcct ggacaagatc acagacactt tgatccacct gatggccaag gcaggcctga
    12001 ccctgcagca gcagcaccag cggctggccc agctcctcct catcctctcc cacatcaggc
    12061 acatgagtaa caaaggcatg gagcatctgt acagcatgaa gtgcaagaac gtggtgcccc
    12121 tctatgacct gctgctggag gcggcggacg cccaccgcct acatgcgccc actagccgtg
    12181 gaggggcatc cgtggaggag acggaccaaa gccacttggc cactgcgggc tctacttcat
    12241 cgcattcctt gcaaaagtat tacatcacgg gggaggcaga gggtttccct gccacagctt
    12301 gatgaagatc tgagctctta attaatgatc ataatcagcc atatcacatc tgtagaggtt
    12361 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
    12421 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
    12481 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
    12541 atcaatgtat cttatcatgt ctggatccgt ttaaactaac tttaaataat tggcattatt
    12601 taaagttaca tatggcggcc aattcgcctt ctgcaggagc gtacagaacc cagggccctg
    12661 gcacccgtgc agaccctggc ccaccccacc tgggcgctca gtgcccaaga gatgtccaca
    12721 cctaggatgt cccgcggtgg gtggggggcc cgagagacgg gcaggccggg ggcaggcctg
    12781 gccatgcggg gccgaaccgg gcactgccca gcgtgggcgc gggggccacg gcgcgcgccc
    12841 ccagcccccg ggcccagcac cccaaggcgg ccaacgccaa aactctccct cctcctcttc
    12901 ctcaatctcg ctctcgctct tttttttttt cgcaaaagga ggggagaggg ggtaaaaaaa
    12961 tgctgcactg tgcggcgaag ccggtgagtg agcggcgcgg ggccaatcag cgtgcgccgt
    13021 tccgaaagtt gccttttatg gctcgagcgg ccgcggcggc gccctataaa acccagcggc
    13081 gcgacgcgcc accaccgccg agaccgcgtc cgccccgcga gcacagagcc tcgcctttgc
    13141 cgatcctcta gagtcgagat ccgccgccac catgattgaa caagatggat tgcacgcagg
    13201 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
    13261 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
    13321 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct
    13381 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
    13441 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
    13501 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
    13561 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
    13621 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
    13681 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
    13741 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
    13801 ccggctgggt gtggccgacc gctatcagga catagcgttg gctacccgtg atattgctga
    13861 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
    13921 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
    13981 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc
    14041 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
    14101 cagcgcgggg atctcatgct ggagttcttc gcccaccccc cggatctaag ctctagataa
    14161 gtaatgatca taatcagcca tatcacatct gtagaggttt tacttgcttt aaaaaacctc
    14221 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
    14281 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
    14341 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
    14401 tggatcccta actttaaata atgccaatta tttaaagtta ggaattaatt cacccagctt
    14461 tcttgtacaa agtggttgat atctctatag tcgcagtagg cggtatcctc aaacccacct
    14521 cagtgtctag caatagggta ttcacactgt attcacactg tattcacact gactactcta
    14581 aaatctaagt ggagtgctag acatttatta gtacgtgacc ccacttggaa atgagtacat
    14641 gaatgtttta aagcatcttt ctttttatac ccggaaatta tcacgttgta aatgcgcgtg
    14701 aagcatcagg atgcttccac tgtccaactg ttcattgttc tgccgcccaa acatcatagt
    14761 tctgagctcc aagtgctttg ggatgcctct gactttctgt aagacaagtt gggtgcagtc
    14821 tgtaagtgac cttttgtgct tagggactct ggaattgccc accagtcatg gcaaaggctt
    14881 ttccttttat tgagcaaagt tcatagtcag acagcggaaa gctctcttgc tgcctgaatc
    14941 ctcaaaactg agctttgctg agttaaatga catgtgagga atatttctga tataaaaatt
    15001 ggaaactttc agatacctcg aaccttgctt atctttgtaa atacacatgt tccaattaaa
    15061 ataggtactg aaacatttgg gtggcacttc aaaactccca atttagattt aatttagatt
    15121 aaagcactta ttctttttta atacagttgt aaaattgatt attaaagtca cctattgaag
    15181 attgaaagca caggatgtat actttccttg tgagacaatc tcatcttcaa taagtgtggt
    15241 tctattaatc aattagctat taaaatataa ctgccatatt aagcctgact catttgtcat
    15301 gacagctggt ataattataa aaattatacc tagccattta tacagccata atcttgcaat
    15361 aaatataaat aattgtctct attaattcat tttgaaggtt ctgtctatta attctccagt
    15421 taagtggcaa tttggggacc ttcatttaaa atctttgttg ttcgttccat ctgactgcca
    15481 gagttccaca cgtgtacctc ccactgttga ttttggaaac caatttatga ataaaatagc
    15541 tttttaagtg caaatattta aagggttgag gcccaaggtc caaggctttc tggggaagaa
    15601 ggtaatgagg gtgcctgtgt ttggtcagga agagggatga gctagtcgtg acattctagt
    15661 attgggttgg ggagagagta tgtggatttc actgtgtgaa cgtctcctaa agcgtgtggt
    15721 ggggtaactg ttcataactt gattctccac aggagtggat gaggtcacca ttgtcaacat
    15781 cctgacaaac cgcagcaatg tgcagaggca ggacattgcc ttcgcctatc agagaaggac
    15841 caaaaaggta ctggaacctt agtgtggagg gcaggccttg ttgagttaat gttctcagat
    15901 attgcctgtt ctgttcttaa ttaatatgtg tgtgtgtgtg tcccttgttt gtgttgccct
    15961 gcataccgta gggtggcagg ccctaaagct tccagatgat tcccctgtct tcaatttgag
    16021 atggcgcaac gcaattaatg ataacttcgt atagcataca ttatacgaag ttatggtctg
    16081 agctcgccat cagttcaatt ctccacccct accccgtcca acattacttt ttctttttgt
    16141 gagttgtgga tcttctttaa ccaaaaggat ccttctagaa ctcttggatg tccaaggttt
    16201 ccctcttata tccttgagag ggttgctgct ttcataggta cagccgtggg aggtagaatg
    16261 gaaaggtgac atcctggaac acacgaggga ggcattctgg aatagacgac aaggaagaga
    16321 agcaatggtc ccacgggtta tgtgctgact ctgtcattgc tgtgcttagg tagcaactca
    16381 cttcctctca tggcttctct ggcttcttcg tcagatgttg tgaatgtaat ttcagaatgg
    16441 tttcctccac tgggcgtgag tcctaacgtg tgcttctcat ttacactgag gggctgggga
    16501 gacagctctg ttggtaaagt gcttgtcaca caagcatgag tatctgagtt caatcttcag
    16561 catccacgtt aaaagagcta aataccccag tgctagggac gctagatagg attcctgggg
    16621 ctccccagac agtcaattta gctgattcag agagctctaa atctcacaca cacacacaca
    16681 cacacacaca cacacacaca cacacagctt ctttatcaga ttcatcgtgg ctgaagtgct
    16741 gtggtccagc ctgctatctt caataggctt cgctcctagt gtttatggag ttttctgtgt
    16801 gtgttttccc tttgctcgtc cactcgtggc taaagcaggc cccgtcttgt ccaccttagt
    16861 ttcccacagc actaggacat tttaccttta aggatatgct acttaatctc taacgctttg
    16921 ctattaaaag cttttgactt atattcggac agtgccatca gggtgacctc atgcggctcc
    16981 cgcctgactt gaggctttca ctccccgcta cagtagcatt gacctgaaag aatgccagga
    17041 gaaactgtgt gtccctggag ttggcgtgac caccaggtcc tgggagttcc aggtcaagcc
    17101 tggctgaagc tctgtcctcc gtgctccaaa gcagttggga aaggacgtaa atgggaaagc
    17161 agctagctcc cttcatccca catgcattac aattctcctt tcacgggatg acgtgtgtta
    17221 atcttctgca gtgaggctca gaagggagtg ttttatgtct ctggctgtcc caaaggatct
    17281 gagagctgca agagccaagt tcccgttgga acaggagatc cactattctg gactgaagga
    17341 aataactgct cattcagtgt ggccttggaa ctgtgtgtgc acactgagct tggcttcatg
    17401 ttaggtagct ggagaacgca gatcctgcag agcctgttgg agtaaacaca ttattagaat
    17461 catgagaagg gtcagcaaac acacaggcat tagcgcgtgc taaggacagc ccactgccct
    17521 gatgatactt aatggtccag ggtgattatc tgtcacagga agatgccgca actgcctgag
    17581 gtaattttct aaaaggacgt cctagtattg ttttaggtgc actgtttgtc aagactggtc
    17641 tgcagcagtg gttcacagtc tgtgggtcat gatctctttc aggggttgag caaccctttc
    17701 acaggactcg cctaagacca ccggaaaaca gatgtttacc tgatgactca gagtagcagc
    17761 aaacacacag ttatgaagta gcaacaaaaa tacttttgtg gctgggatca ccgtggcctt
    17821 tctgaccagg acaaagaacc ctattaaagg gttgcagcac taggaaggtt gcgaaccact
    17881 ggtctagagg gacagaggga ccctgcccag gtggtgagct gctgcagata ctgtcacagc
    17941 tagtaaaaga gggacacagg gaggaagttt gcattttatg ctttgtccct tcaaactagt
    18001 gctagacaca aggtttctct atgcagagaa cagttgttgt tctattgatc ccttctggga
    18061 tttttatttg tgctgagtcc tatactgggc attctgagca attcaagtca aagtggaaaa
    18121 atccagtagg tgccccagat gtcccctgag gtgagagcag agatgggggt gggggcggga
    18181 gggggagtgt caaggacaga ccaagttggg agtcacttaa cctcagggtt ccaatttgga
    18241 aagctggtgt tataattggt atgtgttgtt ttagaaagac cttcctacct cccatgcatg
    18301 gttagggcat cagcagccag aaggaggagg tgaatgggct gggcctaatg cttgttcgca
    18361 tccatgccag gtctgacttt cttgacactt gctgtatgtt acctttagag gcttgtggag
    18421 cctcagaaat tgttggccag ccagcctaca gggccaagtt ccaggccaac aagagacctt
    18481 atctcaatca gaaagtggag ggcacctgag gaatgacaac taagcttgtt ctgtgacctt
    18541 aacccgcata tgctcctaca cacacacaca cacacacaca catgcaacac gcacacccca
    18601 cataaaaata aatgcagttc agttcagtgc agctgtggcc tagtccattc ccatggctgg
    18661 tgagtatcca gtcgccgagg aaatatcttt aagagccacc ctgaattaag gatatctgtc
    18721 tacatatctg agtgctgcct cagacctccg actttattaa aattgtgtgt tcatgatgat
    18781 agtggcgtga tgatgacact gtccaacagg aagttgaaag ggactctgag gggctggagt
    18841 tcacatcata ttcatcaccg ccccaacctg gaggagatgg gagatggctc agggcgaggt
    18901 gcggtgtttt acatagggaa agctcagcac cctccccccc acccccggcc tcgctagcac
    18961 agctagctcc aggctacaag gtcccaccct agagcagcat cacaaacgcc ttccctggga
    19021 cctctataga cctgcaggac tccactggtc tttctacctc acttcttttc agcttgactt
    19081 ggaaatctcc ctgagactcc actcttagct ctcctctcag gagctatcag ctacagctac
    19141 ctagtgatca gcctgggtgt tagtggattc ccttaccatg tggtttctgc cctcctggtc
    19201 tgatgtaggg gacgaggaag ttctccccaa acggcagagt ggtcaaagac tcacacacag
    19261 cctgcttttc agagagagcc atcttatttc tgtctcgctg gttccagagt ggacgttggt
    19321 tccaagcagc tctgagacat ctccctttta caggagctcc cgtcagcgct gaagtcagcc
    19381 ttatctggcc acctggagac ggtgattttg ggcctattga agacacctgc ccagtatgat
    19441 gcttcggaac taaaagcttc catgaaggta aacacgtgtg gggtgtcatc cgtcaacctg
    19501 cccattagcg tctgtgaatt acagcctttt gagtgtaact aggtgattgt cagttgtgtt
    19561 ggagtcttat ttgaagtcat ttccccccaa tgctataagt tgtcaggatt tgaaccaaaa
    19621 atatttcaat ctctctcttt tttaacgacg gcaaagaaat ttcctgatgc ttagcagctt
    19681 agtgatggtt agcagctgct gagactcggc acctgggcga ttggaatgcc tcttgggaaa
    19741 actgcttcct tgattacact tcctttttaa ggaggatatg tgtggtatta aggagaaaga
    19801 aaaacagctt ttgttcagta aagcttgcag attctgccca tgccctcctg gatgctgctt
    19861 cctctggctt ccccctgccc ccccccaatc cgttgctggg ccaaaatttg gatttggtcc
    19921 aaaaatcagc aatgcagtgc aggtgagggt cgatatcagg gtgggactgg cacctgaatc
    19981 caatgggaag tttctgcagg acataatatt gttatctaag ttttgatttt gcaaagctat
    20041 tttgaacatg ggaattgagg ttaaattagt aaattctagt ttagaaacgt ccttggattt
    20101 tgtgctcacc taagctaccc aatattactc aaatgtaaga gatagacaaa tgtctgccac
    20161 agcaaagctc agggcccctt ctctgtgtac ttgtgacatg ttggataatc tgaggctgcc
    20221 ctctgctggt actttttggt aaggcatctt caatgagggg aagctgtatg tcaccctgtt
    20281 gggttgtcat cctttctgga gaacacacta ggaatgaggc cccttcacac atagagtacc
    20341 accagaaccc tggaactgat agcccagagc cactgtagga tttgtcaaat ctctctctgt
    20401 ctctttttta gttcatcagc atagctcaag tggcatccta ggcaggctgt actgcagagg
    20461 aggtaccagt cagtgccgtg acacaccagg cacagggtgc aggctaataa tcctagcact
    20521 ccagaagtgg aggcaggggg atcaaaagct caaggaagtt taaggccagg gtgggatatc
    20581 tgagaccttg tctagagaga gagagagaga gagagagaga gagagagaag aagaagaaga
    20641 aggagaagga gaaggagaag gagaaggaga aggagaagga gaaggagaag gagaaggaga
    20701 agaagaagaa gaagaagaag aagaagaaga agaagaaaga agaaaaggag agtgtacttt
    20761 catgccaaca acagctttga tgatagtagc attcctgaga ttgccaagcc tttgggccat
    20821 tggggtcatg tgtaagccaa ttttttccca ggtttcttta actatcaaca cttggatcag
    20881 ttcatcttat ttttgtcaag aatccactta gttgagattt atgtgatttc actggtatct
    20941 atggtcgggt attattgact taaagc
//