LOCUS       214270#pL1L2GTK_LF2A_nEGFPOT2A_CreERT2_pAbActNeopA#L3L4_pD223_DTA_T_spec        21501 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2GTK_LF2A_nEGFPOT2A_CreERT2_pAbActNeopA
COMMENT     design_id: 214270
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000029521
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            9438..9509
                     /db_xref="ENSEMBL:ENSMUSE00000174322"
                     /rank="1"
                     /note="ENSMUSE00000174322"
     misc_feature    4275..9987
                     /note="5 arm"
     misc_feature    10015..10039
                     /note="B1 Gateway"
     misc_recomb     15999..16030
                     /note="Rox"
     misc_feature    15993..15997
                     /note="for bActNeo"
     insert          13917..15992
                     /source="pL1L2_GTIRES_BetactP FLFL MapVerified"
     promoter        14216..14736
                     /note="human beta actin promoter"
     misc_feature    14612..14631
                     /note="betact5"
     misc_feature    14762..15747
                     /note="neomycin resistance "
     misc_feature    14931..14950
                     /note="neoPstI_5"
     misc_feature    15268..15286
                     /note="neoSphI_5"
     misc_feature    15561..15580
                     /note="neoBstB1_5"
     misc_feature    15812..15835
                     /note="RAF5"
     misc_feature    15754..15997
                     /note="SV40pA"
     misc_feature    15406..15406
                     /note="G to C conservative mutation to kill RsrII site"
     misc_feature    14994..15576
                     /note="synthetic fragment mutates RsRII"
     misc_feature    12952..13896
                     /note="ERT2"
     misc_feature    13920..14162
                     /vntifkey="21"
                     /dnas_title="SV40 pA"
                     /label=SV40 pA
     primer          13977..14000
                     /vntifkey="27"
                     /dnas_title="LRPCR RAF5"
                     /label=LRPCR RAF5
     primer          14103..14121
                     /vntifkey="27"
                     /dnas_title="seq primer NF"
                     /label=seq primer NF
     misc_feature    14163..14170
                     /vntifkey="21"
                     /dnas_title="RE sites"
                     /label=RE sites
     misc_recomb     14171..14202
                     /note="Rox(inv)"
     misc_feature    14203..14210
                     /vntifkey="21"
                     /dnas_title="polylinker"
                     /label=polylinker
     misc_feature    10052..10099
                     /vntifkey="21"
                     /dnas_title="Frt"
                     /label=Frt
                     /note="Frt"
     primer          complement(10064..10087)
                     /vntifkey="27"
                     /dnas_title="FRT3_oligo"
                     /label=FRT3_oligo
                     /note="FRT3"
     intron          10100..10975
                     /vntifkey="21"
                     /dnas_title="mouse En2 intron"
                     /label=mouse En2 intron
                     /note="mouse En2 intron"
     primer          complement(10168..10189)
                     /vntifkey="27"
                     /dnas_title="LRPCR LAR3_EN2"
                     /label=LRPCR LAR3_EN2
                     /note="LRPCR LAR3_EN2"
     misc_feature    11137..11853
                     /note="EGFP"
     misc_feature    11917..12942
                     /note="Cre"
     exon            10975..11037
                     /note="mouse En2 exon"
     misc_feature    10875..10896
                     /note="seq primer FCHK2"
     misc_feature    11027..11038
                     /note="Kosak"
     misc_feature    11863..11916
                     /note="self-cleaving' 2A peptide"
     misc_feature    11913..11913
                     /note=" A to G to kill RsRII site"
     misc_feature    11038..11057
                     /note="FCHK QC suffix region"
     misc_feature    10989..11024
                     /note="FCHK QC prefix region"
     misc_feature    11025..11037
                     /note="FCHK QC critical region"
     misc_feature    11047..11136
                     /note="3X NLS"
     misc_feature    16043..16063
                     /note="B2 Gateway"
     misc_feature    9988..16093
                     /note="Synthetic Cassette"
     exon            16372..17017
                     /db_xref="ENSEMBL:ENSMUSE00000174323"
                     /type="targeted"
                     /rank="2"
                     /note="target exon 1 ENSMUSE00000174323"
     misc_feature    16094..17181
                     /note="Critical Region"
     misc_feature    17205..17238
                     /note="Downstream LoxP"
     misc_feature    17182..17261
                     /note="synthetic loxP region"
     misc_feature    17262..21501
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggaaattta atagtaaaac cccttacata aataggatga ggaaagagtt
     4321 acaaagagtc aatgcaaaac acagatcact aaaactaata catagtaaaa attaaaggga
     4381 agaaaatagt aaaatatttg agaatgggat tacaagtgac attattttca tgagaatggt
     4441 ctttgtatac cctcattcac caatgacaca caaatacttt taatcttttg ttatcagaga
     4501 acacatgtga catcagaact gttgtgttat atgctaggca ttattccaag agcttaatga
     4561 tgattggctc aatctgcaaa tggccctttg agataagcat tccttcttca tctcatatag
     4621 ttttaggata caattataga tgaaatggga tctatagtga ttttgttatc tttacttctg
     4681 aaattccata gcttttcata catttttgtt tttaatttga tacttaaaaa cataaagatt
     4741 atgtgcattg atgggtgtca tttggcacat cagcacattg atgcagattc tttttacagt
     4801 ttcattgtgt gttcacgggc cgagctctgc tctgagatct ttatccgtat tgccttacct
     4861 agggagctag ggatggttat tagtccgatt ttacagagac aattaagcaa gttgcttgag
     4921 aatgcttggg tggagagagc aaagctggaa actggtcctg gaatgtgggt taagactgtg
     4981 catggtgttt cccactacag cccatccacc caggaatgga ccccagacac tgctgctctc
     5041 cccatctcca gttctttccc tgtacctttc attatgaact gtttacttac tgacagctct
     5101 cccagctcaa acaatgttgg tgacttccca ggggatttaa tctacccagg gagcattctt
     5161 tgtagggaaa atacgttttg tcttccatca taggtgcata aaagcttctt gaacaatccc
     5221 tcaaccccct tccccagcat tggaagaaaa gtctctacct tagatcatct atagaaaaga
     5281 atctctcggc cttccccttt gggatggttg ttatagctga agttccactt ctggcaactt
     5341 cagctgaatt ctctcctctc actttaccct ctatatacac atggtccctc tacatttggc
     5401 cagccagagg tctactcttt aggaccatat gtagaaatag ggccttcaat gatttctgcc
     5461 tacttggagg aatttatcaa tgtcaaatgt tcatggattt tttttggcat gtcaaattca
     5521 gacaagaaga gaatcccaac cacctctatc atcttcatta gacctacctg aaatagcatc
     5581 aatagaatat ccagcattct tggtcaaggt tatggcacca tctaagaatg aaaagagcca
     5641 cattagttca ttggcttggg cagggtttta ttttgggttc ttgtccatat tataaaaggg
     5701 ccatttaatt tcctctactt tctattctga agaagaaaca gatgttatct ttaccctctg
     5761 agctctagaa ttgaactggc tcatctcagg aaatagggac catagcagag ctgcttagga
     5821 gactgacaga caagaactgt atgtaatgtg caatgttcta tcaccatcct acaggcttgc
     5881 aattctcaga ggcttttctg tgcattaata cctcagatgc ttatagaggg aggaattaga
     5941 tctttgggat gtaaagggag ggagtcatac atacccctca tgaaagatgt tatcagtatc
     6001 ccacacatgg ggatctcagg tcagagcaga actgtataaa aggcaggcgg ttctcatagc
     6061 acctcaatcc attactcgct gagtgatcta ctccagtgcc tgaggcaggt agaacatctc
     6121 ccagaccaga gagactctga tgctagtttt ctgggcagaa aggggctctg gttggaactg
     6181 agggtaatct tgaagatgaa caaggggaaa agagggttag gagggaaggg tttctctgca
     6241 agtgaaaggt aatggaagat tgtactagta tctttttgac tttacagtga atttatttgg
     6301 aaggaagaag tgcttataca gggatcattt gtacttttta ttctcgaaat aaatttaaga
     6361 gattgggttt tcaaacttct aactatttta gagataagat actgtaaatt catagttcag
     6421 tcttcaaagc aacatttgag ctgttgacct tatatttttt tgtattattt ttcactttta
     6481 tccagttcct ttggagatgt cctgtcagca gagccagcaa cagtgccagc cccctcccaa
     6541 atgcaagacc ccaaagtgcc ctccaacctc ttcctgctgt agcctgggtt ctgggggctg
     6601 ctgtggttcc agctccgggg gctgctgcag ctctggatgt ggtggctgct gtctgagcca
     6661 ccacaagccc cgattctctc tccgtcgccg acgtcacagc tctggatgct gtagcagcgg
     6721 tggcagcagt tgctgtggca gcagcggtgg cagcagttgc tgtggcagca gcggtggcag
     6781 cagcggtggc agcagcggtg gcagcagcgg tggcagcagt tgctgtggca gcagcggtgg
     6841 cagcagcggt ggcagcagtt gctgtggcag cagcagcggt ggcagcagtt gctgtggcag
     6901 cagcagtggt ggcagtagcg gtggcagtag cggtggcagc agctgctgtg gtagcagtgg
     6961 gggcagcagt ggtggcagca gcagtggcag caacagccat cagcagtctg ggggctctag
     7021 ctgctgctct ggaggttgct gctgacctgg ccacgaagaa catggaaagc agcattgact
     7081 gaagacctgc aagcctaggg tctcctcttc tctggttttc ccgcagaagt acttagaact
     7141 ctctgtacgt cctcttcatc tccctaaagc tcatctacca cgaggtcttc aggtcccagc
     7201 tctgagatgc attggctttg ctttttttct ctgacacaac taattgagaa tgagatattg
     7261 aaaaattagc tttgaaaaat ctttttctgg gcaataataa agcttttgat tcctgacacc
     7321 atggcctccc aatgtacttt tgtttccctc ttccaattcc ctccctccac accaagtcat
     7381 agtccctttc gttatctaaa tcacacattt tgacactgaa tccacttaaa acttagtact
     7441 ctgaggcctg tgccccttat accaacttgc acagggggga caaggcatag gttaggacaa
     7501 gcagtaccca gaaaagctcc atgatgggac ggtgacactc aaaagggacc catgatttga
     7561 aaaagtttat ctcttaatca tggagaaaaa gattatctgg agtgcacatt gtgggctctc
     7621 atgagtagac acactgttgt ctgattatcg ccagctggcc aggcagggaa gctgagtgca
     7681 gagcatttct agagaagtaa gctgaggaga cagctgtgaa atgttgggag tccactattc
     7741 ttggaacctt agaattttag gtttgggttt gaatagagaa taaagaaacc atctcagcca
     7801 aaaattaccc ccgcctggtg gtagtggtgg tggtgcatgt gtgcatgcct gtgtatgtgt
     7861 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgatggagt caagggtact
     7921 taagtcactg agtttggatg ttcatgtaat attcctttag ggttgggatg gcttcctcag
     7981 gagatactaa aaatatttat gatagaatag ataactgcaa gtctgccttt ctatacaaga
     8041 taagcagtta gctcctcggt tgataagagt taacagtgat gcataaagtt ggggtaagga
     8101 gtccatacag tgagtatcta cttgatctct ccataatgat tcctatcacc ctggttttct
     8161 tcaccttttg cataattttc cagaggagaa cccacatcta acttagtact tcattacttc
     8221 cttctctttt ctaacattga tctctcttct tggaatgtct tacctcttct tgccaggtgc
     8281 tctggttcag taagcaacct gttaatcagg ctctaaagcc atggtaaatg aaatgggtat
     8341 cttctatgac ttgtctttcc ttgtctgttt cctcatgcca cacccagcaa tgcaccccaa
     8401 aacctatctc actcttaatc cctgaattct taatcttaga ttctcttatg tctcatactc
     8461 atggttggtg tgaccaggta gttccaatga ttgaatcaga ggcagttgac ctccaaagtt
     8521 tcctccagtt ttataaatca gctaatcgaa acgcaacaat tactatggga actttcaggc
     8581 cgttccccct tttattttcc tcctttttct tctttgctct tttattttat ccttttcatt
     8641 ttgttttgtt ttattttgtc tttttcctat ttgtcagtag ttctagggac aatctatcct
     8701 taaggagatg atatcagaaa caggagctcc ataggctaac cagcttacta ttttccaata
     8761 ggcctatact cctcaaccat ttttgtctgc gatccatcag ggttacataa tgggaatatg
     8821 gtcaagtgac ttcttttggt cttattggtc ccttaacctt gcctgggcta ttcttcctgt
     8881 taatggtgac aggaagaaac caccgttgtg atttccatcc ccaccatcat cttccttcag
     8941 tgctctctag aagcatctgg ataaaagcca gagggttcat cacatagcct gcagcctgct
     9001 atgaaagaca agaaccaagg gccatagaga tgcaaataag acaataaatg ataaggagat
     9061 agctatacat gaataagtgt gagaaagtag aacagtgcag gtgttctcct cccctcctgc
     9121 tgtgtgtgtg tatacagtgt ttaagagaga accccggacc tctcctcatt tgaccccccc
     9181 cccccaggcc ttctctaagc acatacagcc ctcccagcta tgaacagtat ccggtttgaa
     9241 aagtaaacac accacagcaa tcctggcacc tctgtgaagt gacttggttg tccacgtgca
     9301 ccacctacag tgctatctga gtgatgaatg cttctgggat gtaagtgtca gctgagcatg
     9361 tctgttgaga aatgggctcc tcccaaggac ctggacttgt tggaatctta taaaagcttc
     9421 tctggccata cactgttcag tcttctgcct agcaggtgtc aagttccagt tggagaactg
     9481 agtgagtctt cctgtggagc caaggtctag taagtatcta agcggggtgt ctgggggagg
     9541 aagctgggga gaatggaaga cagaaaggag gaagtaggat gcagccagaa tgtagctgtt
     9601 ggaatctggc ttccagtgag ccagcctttt gtgttgccta tgaagatgca aaggcttggc
     9661 atggcgctga cctttgaaag gttttccaga ctgtaaagag ctttgagtaa cccgggtctt
     9721 taggttccct ggttaagtta ttcctattct ctgccttttc cttataatgt gaaggacagg
     9781 caagaaagaa ggtgcagagc acgcttttgt gttttcgaat tctctatacc caagacagag
     9841 ggacacaggt aatacttgtg tgttttctga ccgatgtgat gcttatacga tattaactga
     9901 gattaccact tgaaagagct gcgtggtcaa agtacagtta ggaaaaggct gaagccaaga
     9961 gatttttgat tgagtcatat agataaaaag gcgcataacg ataccacgat atcaacaagt
    10021 ttgtacaaaa aagcaggctg gcgccggaac cgaagttcct attccgaagt tcctattctc
    10081 tagaaagtat aggaacttcg aaccctttcc cacaccaccc tccacacttg ccccaaacac
    10141 tgccaactat gtaggaggaa ggggttggga ctaacagaag aacccgttgt ggggaagctg
    10201 ttgggagggt cactttatgt tcttgcccaa ggtcagttgg gtggcctgct tctgatgagg
    10261 tggtcccaag gtctggggta gaaggtgaga gggacaggcc accaaggtca gccccccccc
    10321 cctatcccat aggagccagg tccctctcct ggacaggaag actgaagggg agatgccaga
    10381 gactcagtga agcctggggt accctattgg agtccttcaa ggaaacaaac ttggcctcac
    10441 caggcctcag ccttggctcc tcctgggaac tctactgccc ttgggatccc cttgtagttg
    10501 tgggttacat aggaaggggg acgggattcc ccttgactgg ctagcctact cttttcttca
    10561 gtcttctcca tctcctctca cctgtctctc gaccctttcc ctaggataga cttggaaaaa
    10621 gataagggga gaaaacaaat gcaaacgagg ccagaaagat tttggctggg cattccttcc
    10681 gctagctttt attgggatcc cctagtttgt gataggcctt ttagctacat ctgccaatcc
    10741 atctcatttt cacacacaca cacaccactt tccttctggt cagtgggcac atgtccagcc
    10801 tcaagtttat atcaccaccc ccaatgccca acacttgtat ggccttgggc gggtcatccc
    10861 cccccccacc cccagtatct gcaacctcaa gctagcttgg gtgcgttggt tgtggataag
    10921 tagctagact ccagcaacca gtaacctctg ccctttctcc tccatgacaa ccaggtccca
    10981 ggtcccgaaa accaaagaag aagaacgcag atcataactg actaggaggc caccatgggc
    11041 ggaccggtgc atgtggaccc caagaaaaag cggaaggtgg accctaaaaa gaaacgcaaa
    11101 gtggatccta agaaaaaaag aaaggtgcac gtgtccatgg tgtccaaggg cgaggaactg
    11161 ttcaccggcg tggtgcccat cctggtggaa ctggatggcg acgtgaacgg ccacaagttc
    11221 agcgtgtccg gcgagggcga aggcgacgcc acatacggaa agctgaccct gaagttcatc
    11281 tgcaccaccg gcaagctgcc cgtgccttgg cctaccctcg tgaccacact gacctacggc
    11341 gtgcagtgct tcagcagata ccccgaccat atgaagcagc acgacttctt caagagcgcc
    11401 atgcccgagg gctacgtgca ggaaagaacc atcttcttta aggacgacgg caactacaag
    11461 accagggccg aagtgaagtt cgagggcgac accctcgtga acagaatcga gctgaagggc
    11521 atcgacttca aagaggacgg caacatcctg ggccacaagc tggagtacaa ctacaacagc
    11581 cacaacgtgt acatcatggc cgacaagcag aaaaacggca tcaaagtgaa cttcaagatc
    11641 cggcacaaca tcgaggacgg ctccgtgcag ctggccgacc actaccagca gaacacccct
    11701 atcggcgacg gccctgtgct gctgcctgac aaccactacc tgagcaccca gagcgccctg
    11761 agcaaggacc ccaacgagaa gagggaccac atggtgctgc tggaattcgt gaccgccgct
    11821 ggcatcaccc tgggcatgga cgagctgtac aagcctgcag gcgagggcag aggaagtctt
    11881 ctaacatgcg gtgacgtgga ggagaatccc gggccgtcca atttactgac cgtacaccaa
    11941 aatttgcctg cattaccggt cgatgcaacg agtgatgagg ttcgcaagaa cctgatggac
    12001 atgttcaggg atcgccaggc gttttctgag catacctgga aaatgcttct gtccgtttgc
    12061 cggtcgtggg cggcatggtg caagttgaat aaccggaaat ggtttcccgc agaacctgaa
    12121 gatgttcgcg attatcttct atatcttcag gcgcgcggtc tggcagtaaa aactatccag
    12181 caacatttgg gccagctaaa catgcttcat cgtcggtctg ggctgccacg accaagtgac
    12241 agcaatgctg tttcactggt tatgcggcgg atccgaaaag aaaacgttga tgccggtgaa
    12301 cgtgcaaaac aggctctagc gttcgaacgc actgatttcg accaggttcg ttcactcatg
    12361 gaaaatagcg accgctgcca ggatataagg aatctggcat ttctggggat tgcttataac
    12421 accctgttac gtatagccga aattgccagg atcagggtta aagatatctc acgtactgac
    12481 ggtgggagaa tgttaatcca tattggcaga acgaaaacgc tggttagcac cgcaggtgta
    12541 gagaaggcac ttagcctggg ggtaactaaa ctggtcgagc gatggatttc cgtctctggt
    12601 gtagctgatg atccgaataa ctacctgttt tgccgggtca gaaaaaatgg tgttgccgcg
    12661 ccatctgcca ccagccagct atcaactcgc gccctggaag ggatttttga agcaactcat
    12721 cgattgattt acggcgctaa ggatgactct ggtcagagat acctggcctg gtctggacac
    12781 agtgcccgtg tcggagccgc gcgagatatg gcccgcgctg gagtttcaat accggagatc
    12841 atgcaagctg gtggctggac caatgtaaat attgtcatga actatatccg taacctggat
    12901 agtgaaacag gggcaatggt gcgcctgctg gaagatggcg atctcgagcc atctgctgga
    12961 gacatgagag ctgccaacct ttggccaagc ccgctcatga tcaaacgctc taagaagaac
    13021 agcctggcct tgtccctgac ggccgaccag atggtcagtg ccttgttgga tgctgagccc
    13081 cccatactct attccgagta tgatcctacc agacccttca gtgaagcttc gatgatgggc
    13141 ttactgacca acctggcaga cagggagctg gttcacatga tcaactgggc gaagagggtg
    13201 ccaggctttg tggatttgac cctccatgat caggtccacc ttctagaatg tgcctggcta
    13261 gagatcctga tgattggtct cgtctggcgc tccatggagc acccagtgaa gctactgttt
    13321 gctcctaact tgctcttgga caggaaccag ggaaaatgtg tagagggcat ggtggagatc
    13381 ttcgacatgc tgctggctac atcatctcgg ttccgcatga tgaatctgca gggagaggag
    13441 tttgtgtgcc tcaaatctat tattttgctt aattctggag tgtacacatt tctgtccagc
    13501 accctgaagt ctctggaaga gaaggaccat atccaccgag tcctggacaa gatcacagac
    13561 actttgatcc acctgatggc caaggcaggc ctgaccctgc agcagcagca ccagcggctg
    13621 gcccagctcc tcctcatcct ctcccacatc aggcacatga gtaacaaagg catggagcat
    13681 ctgtacagca tgaagtgcaa gaacgtggtg cccctctatg acctgctgct ggaggcggcg
    13741 gacgcccacc gcctacatgc gcccactagc cgtggagggg catccgtgga ggagacggac
    13801 caaagccact tggccactgc gggctctact tcatcgcatt ccttgcaaaa gtattacatc
    13861 acgggggagg cagagggttt ccctgccaca gcttgatgaa gatctgagct cttaattaat
    13921 gatcataatc agccatatca catctgtaga ggttttactt gctttaaaaa acctcccaca
    13981 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
    14041 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
    14101 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
    14161 ccgtttaaac taactttaaa taattggcat tatttaaagt tacatatggc aattcgcctt
    14221 ctgcaggagc gtacagaacc cagggccctg gcacccgtgc agaccctggc ccaccccacc
    14281 tgggcgctca gtgcccaaga gatgtccaca cctaggatgt cccgcggtgg gtggggggcc
    14341 cgagagacgg gcaggccggg ggcaggcctg gccatgcggg gccgaaccgg gcactgccca
    14401 gcgtgggcgc gggggccacg gcgcgcgccc ccagcccccg ggcccagcac cccaaggcgg
    14461 ccaacgccaa aactctccct cctcctcttc ctcaatctcg ctctcgctct tttttttttt
    14521 cgcaaaagga ggggagaggg ggtaaaaaaa tgctgcactg tgcggcgaag ccggtgagtg
    14581 agcggcgcgg ggccaatcag cgtgcgccgt tccgaaagtt gccttttatg gctcgagcgg
    14641 ccgcggcggc gccctataaa acccagcggc gcgacgcgcc accaccgccg agaccgcgtc
    14701 cgccccgcga gcacagagcc tcgcctttgc cgatcctcta gagtcgagat ccgccgccac
    14761 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
    14821 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
    14881 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
    14941 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
    15001 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
    15061 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
    15121 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
    15181 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
    15241 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
    15301 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
    15361 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggccgacc gctatcagga
    15421 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
    15481 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
    15541 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
    15601 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
    15661 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
    15721 gcccaccccc cggatctaag ctctagataa gtaatgatca taatcagcca tatcacatct
    15781 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
    15841 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
    15901 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
    15961 tccaaactca tcaatgtatc ttatcatgtc tggatcccta actttaaata atgccaatta
    16021 tttaaagtta ggaattaatt cacccagctt tcttgtacaa agtggttgat atctctatag
    16081 tcgcagtagg cggtatgctc tttattttcc atcatggagc tcttggatgg tcctaatgtc
    16141 agtcagggag actgcactga tccttttcaa attctgtttc ctgctttgtg cctccaccac
    16201 tggaagctcc tgtttgagtt tgtttctttg tttcccttct ttttctttct ttcttttatt
    16261 cctcttgtcc caaagccaga cagacttttc tctaaggcaa atacttaaag ttctgtgttc
    16321 cactataggg aagaaaaggc ggggttttaa aaaagattgt ctttctttca gattcatctt
    16381 gacaaacgcc gagatgtctc aacagggcgc caaaggcttt tccaaagggt cttcccagag
    16441 ttccgccccc tgccccgccc cgactcctgc ccccgcgtcc tcctcctcct cttgctgtgg
    16501 cggaggctgc tgcaggggct gcgggggcgg ctgcggtggt gactctggct gctgcggctc
    16561 cagttccacc ggctgctgct gctttcccag gagacgccgc cgacagcgct ctggcggctg
    16621 cggctgctgc tgcggaggaa gccaaaggtc tcaacgctcc tgcaacaatc agagttcagg
    16681 ctgctgctcc ggtggctgct gaaagcctac agagcttctc cggaacagct ggcatcgcac
    16741 agcgtctttg ctgctctcac ccagtcctcc atagtagtaa caggaaggac cggcctagga
    16801 gtttggtttc ccttaagcta actgcacttt gatgttcaga aacttcaccc tgttcttaga
    16861 cctgtcctct aacgttgatt ttcctcccag gggatacaag tcaggggtgg gacttcagat
    16921 cacgctatgg gcaggaggtt gtgcacagca gtgttcaaaa caggcctcct gatttgattg
    16981 tcgcctggag acataataat aaagcttcta cctcctatga acgcttcttt tcctatttat
    17041 tcctccatcc gtgccttcag ttttcttatt ttatactacc tacccctcaa aggctcatgc
    17101 ctgaaggaga acaaacacat taactctctg cgagttctca ggatttgggg taggcagtct
    17161 catctacttt gtgtaatgac tgagatggcg caacgcaatt aatgataact tcgtatagca
    17221 tacattatac gaagttatgg tctgagctcg ccatcagttc aaagattccg gagatcattt
    17281 tgttaattgg tgtgcttagc ttggtctact cgaagtgatt tggaaactcg gagttcctcc
    17341 tagagcaagc cttgcccagt ccccaggccc agacaaccga gggagtctgg tttgaaatgg
    17401 caagtttagg aatttgaatt cagttctgtt gaatgttttc tcttcgttcc tcttttcttt
    17461 tcctcccttc ttttctactt ctcccccccc ccttccctat ccttttctca tttttctttt
    17521 tctggtggaa aacaattgac ttttgcttgc tggatgagaa aattttctat gcttaccagg
    17581 agcctggaac tcaccatctt ttatgtctga tctatgaagc tgaggcatga ccaggaacca
    17641 caggactcct ctgtttgtgg cagttggaac aaactgtact agaatagaag tctttgtaag
    17701 catagagttt actgttaagg ttaggtaaga cctcttagcc tccctcatgt cttttagctt
    17761 cagaagagat tgtataataa tgataaaaaa aataggacaa aactagctaa catttgttga
    17821 atattaattt tgttccagac acaaggtata gcacagaatg tatgtatgta tattcattta
    17881 attttaataa tctcaagaat aggttttgat tttcctagtt ttaaaagtgt gagtggattg
    17941 gggtatctgt gccaagtttc acaggcagtg agtgtcctag gcctctgatg gtgaatcatg
    18001 gggctaagac ttaaactgag atctacaggc attgaagtcc acggaatcca ctcagggcca
    18061 ctgaatccac tctactgcaa tcctggaaac caccacttcc tgaaagaagg ggcacttggg
    18121 cttctatgga cacacccgag ctcttagaaa attgcagctt gactgctttg catcagtgat
    18181 tgctgagctc tttgtggcac ctgcccagca gccatctgct gaagtatttg ttcagaggca
    18241 aaggaagaaa tgcatcactg attcatccat gctgtttaaa gctgagacta gatgacatcc
    18301 tatgttgggg atggttggaa ggacactcag tccttctctg ccactcactt cgggttctga
    18361 gttcggtgtt tccgtgcttt aattgcactg tttacaaata gatgatctca ttaaggacct
    18421 gcctcctcca taccagtcag tgacgtgttg tgaagcaaca ggcctcatga cttgttggac
    18481 agcaaggcag gaagggtggg cttgcctgac tcagaacata ctggaatggc agtgtgagga
    18541 acacagaatg caaggacttg agaaagagaa atggtaactc attatgaaat atgtgagtct
    18601 ctagcaatac atgtctttga ccattgatag atcaattggt cagttgtgag gatggcagcc
    18661 tcactggttc cctcttatct gtgaaataca gacatgtggg agatacgcaa gaaaacatgg
    18721 ttatggattc ttccttccca agcagcccaa ggcttcaggt gtttaaggta tcgttctggt
    18781 tatataggca gttgaccatt gtcttcacta aaaaagaaac ctgggattcc agctgcttgg
    18841 catacagatt tgctactaat atcagtctta cactgtgaac ttggcactct ttcatttact
    18901 gcttaaaaca ctgccacaag cgaagtgttt ccattgtttc ttgataagga aagggaagca
    18961 gagaggcatc ttaagatgtc acaacttcta ataagaggac aaagttttga acagggtgtt
    19021 tctgatacca aaatcatcat tatggacacc tttttgtact atgcaattct aagatggatg
    19081 aagtcttctg atggaagcaa gagaaagaat ccaaggtgtt gctggaatta aaaaatagat
    19141 ctctgaaaac atgtcctcag aggagagagg agaggccagg ctacaggtgg ctccaaatgc
    19201 caattttagg aatttaacag tgttggattt ggcatagaaa aacggtttct aaagaagaat
    19261 ttggaaagac aaaaattgac ctacagaata tccacagcaa taattaggat gctctggaaa
    19321 cttagaaaga ctatgtatca ataggcagct gcagtgaccc aggcctgaga taagaatctg
    19381 aacatggcat tgggcaaggg aaggaaatga caggtgagaa aagcatttgc ctagagaaaa
    19441 aaaaaataga gagaactggg agtagcattg gggtatatga agacaaacca attaaggtgt
    19501 cattgtggct ttgtgcctgt cactagaaaa tgacagtaac attgacagag aagtggagag
    19561 tgaaagaaga ggtgtgggta gaaaggggaa tcccacccct aacatgctgg atttgaggca
    19621 ctgccagagt atgaacaaaa atcagtgctg tgaagcaaaa aaaaaaaaag cagataatct
    19681 ttgaaaattg atttgacaac atctggataa agaggaataa actatgtgga ggtaggaacc
    19741 caatctttgg catgaatgta gcttcacttc tgcaatgaaa tttatgaaga agaagaagaa
    19801 gaagaagaag aagaagaaga agaagaagaa gaagaagaag gaggaggagg aggaggagga
    19861 ggaggaggag gaggaggagg aggaggaaga ggaggaggag ggagaggagg aggaggagga
    19921 ggaggaagaa aaggagagga agaagaagaa agacctggta tttctaactc atcctagaaa
    19981 gcttgtgtaa aactagaaaa ttagacactg aacactggat atggcctgac tgatatgtat
    20041 tgcttagtca accttggcac cataagacac ctaaggtgaa ttgaggggaa tgagcaggaa
    20101 gaagtggaat tccaaaaggg ggtcaaagat gaaggcagaa aactcactca ggctttcctt
    20161 gggcagctca tcaggctgct ttcaagtctt gggttctaga aattcaggct gaagaagagc
    20221 aggacagagc aaggttgcag aaggaaaagg ttgtcaccta gtaaggtcag tcccatgggg
    20281 cttctttccc attgcttagt caactctgtc accattagaa acagcaagaa gttccatttg
    20341 gagggacagc agttccttcc tccttgattt ggtagaactt gttaaacagg caaattggta
    20401 tcacagtgag taaggagaaa aatcctctct gctgtggatg acactgcagc agactcaaat
    20461 gatttggctg tgataaatct ctactctgtc agcttgcctc aggataaatg gcagccctag
    20521 aaaggcaagt atcacctcct ctcttataaa tactgaccag ggtgaggaca aaggcttcta
    20581 atctatctta ttctgtgatg catcttgtta agaatgtcgt tagaaggatg agggagatgg
    20641 ttcactggaa aagaggaacg gtgttcaaat cccctcctat gtaaaagaaa acaaaagcaa
    20701 aatctgggca tggctgtgca ttcctgtaac cccagcactg gggagcagaa acaggcagat
    20761 cctgggagag cttgctagcc agctggctta ggtgaaacgg taagcttcca gttttcaagg
    20821 caggaagaag aaagcaataa agagagtaga catcttgctt tgacctctac attcatggat
    20881 ccatacatct gcatgttcat acatctgcat gtgtgcctca tatataccac agcactcact
    20941 caccacagac agacagtgtg tgtctgttag ttttattgtc agtgatgagt tgacaataaa
    21001 gctgtgttgg atctacttat ttgtgaattg aatttgcagt gttcaaagac catttttctt
    21061 gcttccaacc cccaaaccac ttttttttcc cctgccggtt tgaagtataa ttacatatac
    21121 ctgaaaacta gaagaaatta acctccttct gtttcatgcc cttatgtgat tttctgttca
    21181 ttcatcactg taatctttca ttcattgaaa atggattgat tggtcaccat tcccttcata
    21241 cttaatgatg taaaaaaaaa agttaatggt gagagttaaa tagtagtgtg ataagtgctt
    21301 aaagccatca ccacatgaca tcatgactat acacaagtga cagttcacac tggtctcaac
    21361 ggaaagagtt gtttagactt aatgctggag atggtgttgg tcaagctgag aacatagaaa
    21421 aaatatttaa gttaaagaaa acagcaccaa gggtgaagac agagagataa acacatattt
    21481 tatagcaaat tctaaatgaa a
//