LOCUS       33765#pL1L2GT0_LF2A_nEGFPOT2A_CreERT2_pAbActNeopA#L3L4_pD223_DTA_T_spec        20676 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2GT0_LF2A_nEGFPOT2A_CreERT2_pAbActNeopA
COMMENT     design_id: 33765
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000020706
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     misc_feature    4275..9101
                     /note="5 arm"
     misc_feature    9129..9153
                     /note="B1 Gateway"
     misc_feature    10120..10123
                     /note="FCHK QC critical region"
     misc_feature    10104..10119
                     /note="FCHK QC prefix region"
     misc_feature    10124..10129
                     /note="FCHK QC suffix region"
     misc_feature    10252..10303
                     /note="F2A"
     misc_feature    10129..10251
                     /note="1Dleader"
     primer          9989..10010
                     /vntifkey="27"
                     /dnas_title="seq primer FCHK2"
                     /label=seq primer FCHK2
                     /note="seq primer FCHK2"
     exon            10089..10128
                     /vntifkey="61"
                     /dnas_title="mouse En2 exon"
                     /label=mouse En2 exon
                     /note="mouse En2 exon"
     misc_feature    11175..11175
                     /note=" A to G to kill RsRII site"
     misc_feature    11125..11178
                     /note="self-cleaving' 2A peptide"
     misc_feature    11179..12204
                     /note="Cre"
     misc_feature    10309..10398
                     /note="3X NLS"
     misc_feature    10399..11115
                     /note="EGFP"
     primer          complement(9282..9303)
                     /vntifkey="27"
                     /dnas_title="LRPCR LAR3_EN2"
                     /label=LRPCR LAR3_EN2
                     /note="LRPCR LAR3_EN2"
     intron          9214..10088
                     /vntifkey="21"
                     /dnas_title="mouse En2 intron"
                     /label=mouse En2 intron
                     /note="mouse En2 intron"
     primer          complement(9178..9201)
                     /vntifkey="27"
                     /dnas_title="FRT3_oligo"
                     /label=FRT3_oligo
                     /note="FRT3"
     misc_feature    9166..9213
                     /vntifkey="21"
                     /dnas_title="Frt"
                     /label=Frt
                     /note="Frt"
     misc_feature    13465..13472
                     /vntifkey="21"
                     /dnas_title="polylinker"
                     /label=polylinker
     misc_recomb     13433..13464
                     /note="Rox(inv)"
     misc_feature    13425..13432
                     /vntifkey="21"
                     /dnas_title="RE sites"
                     /label=RE sites
     primer          13365..13383
                     /vntifkey="27"
                     /dnas_title="seq primer NF"
                     /label=seq primer NF
     primer          13239..13262
                     /vntifkey="27"
                     /dnas_title="LRPCR RAF5"
                     /label=LRPCR RAF5
     misc_feature    13182..13424
                     /vntifkey="21"
                     /dnas_title="SV40 pA"
                     /label=SV40 pA
     misc_feature    12214..13158
                     /note="ERT2"
     misc_feature    14260..14842
                     /note="synthetic fragment mutates RsRII"
     misc_feature    14672..14672
                     /note="G to C conservative mutation to kill RsrII site"
     misc_feature    15020..15263
                     /note="SV40pA"
     misc_feature    15078..15101
                     /note="RAF5"
     misc_feature    14827..14846
                     /note="neoBstB1_5"
     misc_feature    14534..14552
                     /note="neoSphI_5"
     misc_feature    14197..14216
                     /note="neoPstI_5"
     misc_feature    14028..15013
                     /note="neomycin resistance "
     misc_feature    13878..13897
                     /note="betact5"
     promoter        13482..14002
                     /note="human beta actin promoter"
     insert          13179..15258
                     /source="pL1L2_GTIRES_BetactP FLFL MapVerified"
     misc_feature    15259..15263
                     /note="for bActNeo"
     misc_recomb     15265..15296
                     /note="Rox"
     misc_feature    15309..15329
                     /note="B2 Gateway"
     misc_feature    9102..15359
                     /note="Synthetic Cassette"
     exon            15769..15887
                     /db_xref="ENSEMBL:ENSMUSE00000447959"
                     /type="targeted"
                     /rank="3"
                     /note="target exon 1 ENSMUSE00000447959"
     misc_feature    15360..16064
                     /note="Critical Region"
     misc_feature    16088..16121
                     /note="Downstream LoxP"
     misc_feature    16065..16144
                     /note="synthetic loxP region"
     misc_feature    16145..20676
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggatcttct ttttcctcat ccatgtcaga atttgatata attcggtttt
     4321 taacctacgc cattctaact taggtaagat gaaatctcga agtaatttta atttgcattc
     4381 ccacatatct aaggattttg aatacttaaa atatttctta gccatttgca tttttccttc
     4441 agcaactctg tttcattcct tgctttttta tttttacttt ttctggttat agttttttct
     4501 ttgactattc taaatatcaa cttttgttta gatgtagcgc tggtaaaact ttttcctatt
     4561 gtgtggctgc cacctcactt gagtggtggt gtcctttgct ctacagaagt ttttagtttc
     4621 gtgaggtcca tttgtcagtt gttggtcttc atgcctgtgc agtcctgctc agaaagccct
     4681 ttcctgtgtc catcagttgc cgggtacaca ctgcttcttc ctctatcaga ttcagggaat
     4741 caggtcttac gtgtagtcct tggtccattt ggagttgagt attgtacagg gtcagagata
     4801 aggatctagt ttcattcttc tacactcgaa taatcagttt gatcagccct acttgttaaa
     4861 gatgctatct ttcctctaat gtgtatttct ggcctctgtc taaaaatcag tttcccccta
     4921 ttttttgaga taggctttct cattgaacct ggagctctgg atggtcagag gaccctccct
     4981 tatgggatgc atctattttc tgcctctctc ccacctctca cactgctggg cttacagatg
     5041 catgtcacca tgcctgtctt ctatgtgggt gctaggatct gacctgtaat cttcgtgttt
     5101 gtgcaccatg cccttcacct gctgagtcat cttccagcct ctctttagtc acccttgcca
     5161 tgactgcttt aaagtgtgtg ttaattgttc tggcgtctgt cctcttaatg tcagtgtgtg
     5221 ctcgttgtct ttgttcactg aacactgagt gcatgttgcc tagttttggt tattatgaat
     5281 tacatcctgc tggcatctag acatctagat tatattgtat gaaactctat tttatttggg
     5341 tcttgtgttg ggatgctttt ctatgacatc atcctggctg agagaggagt tgttctgata
     5401 ctgtcaggtg aagaaagaga tccctctgtg gccttctctg acatcatccc atgttaagga
     5461 ccagatggag ctctggcctt ggtgcttaac ttttgccaga atggattgat catggcatca
     5521 agttttcaga gtctgtggcc tggagtagca cacttaacca tccaaaagtt tcttcttgag
     5581 aactgccagg ttctgggact tgtctagagt aggcagcttt tgaccatctt tgttgtttgt
     5641 tttgtttgtg cccagtggaa ttccaagttc cttcttctcc ggctccgtgt ttgggaaatg
     5701 aaccccagcg gcctcattcc cagagcatgt cttggcctct gccttcttcc ttccaacttt
     5761 cagactcatg atgttagata tgaatatcca tgacttttac tggtgttctc tgagagaaac
     5821 agggagagat ccttctccat tttccccaga aacagagccc tcacaatcat gtaagaatct
     5881 gatttgggga cagagagatg acacagttag taaagtccct gatttataag catgcagatc
     5941 tgagtttgga tccctagtac ccatgtaaga aaggtgtgtg tggttcagtc ctctgtaacc
     6001 ctgagactgg agactggggg agtagacagg cagatctcta gagctctttt ccaggtagtc
     6061 ttgccgttgg tgagtgctgg gttccgtgag agtctgtttc aaaagataag tggagaatgg
     6121 ttaaagaaat caggctgttg gcctctagcc tccatacaca atgcacatgt gtatgcacaa
     6181 aatgtgcgcg tgtgcacaca cacaccccga cacccacacg tatctaattt ttattgagag
     6241 taacaagtaa tgcaggtcac agttggaata catgcatatt aaggtttgaa catggttttc
     6301 gtggggcact agtatgcttt ctctgataca catttgcact agcttttgtc tgtctgatat
     6361 tttgcttttg atcttatttt ctttgttagg ttttattgct gtcataacct gttctgatgt
     6421 tggttgtgtt gaggcttcag ggttggactt gagattggaa attaacttca gatattgatt
     6481 atctttataa tgtgaatggg ctactttatc cttgaagatt tactcttgag tcagaatttt
     6541 atcttcttgt ccacatgtag ctgctcgggc atctggagaa ccctggtaac caagagtccc
     6601 catgctaagg atgctagcag tagagcagcg taatgggaca gccaactgac caccgactgc
     6661 catagctact tcttgtagtg cccaggacac tggtctagtt ttccaaacta gtgctaggtc
     6721 tagccgcttt tctgttttcc ctctgggttg tccccattgg ccccaggaca ctctagttcc
     6781 tggagggcag tctccccact gactgctcca gccttgtggc ctgcagtctg tttgcttgta
     6841 ctgatgatat ctgcaggcag tgacgctata gccttaagtt tccctatagg cttatggtga
     6901 gagcactgtg gggttgtcag gagggatgtg ggagaatatt aattgtggag gaagttgtgt
     6961 gtggattctg ggtgaaggat agaggtttgt tttgtttgtt tgtttatggt ttttttttct
     7021 ggcttgattt cactttgagc cactttcaca tttaggaatt gcagattagt gacatctgag
     7081 aagaagtaag cccagtgctg gagtggtatg ggggtgcctg atgggagcag tggccacaca
     7141 ataattgtgg ggctgcctag ggggagcagt ggctgcacac tgaatgtggg ggtgcctgga
     7201 gggagcagta gccccacact gaatgtgggg gtgcctggtg ggagcagtgg ccccacactg
     7261 aatgtggggg ctcctggggg agtagtagct gcccactgaa tttgggggta tctgcacact
     7321 gaatatgggg tgtgtggtgg gagcagtggc cccacactga atggggggtg cctggggtga
     7381 gcacttcctg cacactgaat tgtgggggtg cctggtggga gcagtgtcca cacactgaat
     7441 gataaattat gcaaggacaa ggcctaagca gtgatcttgg ttacagtcat aggttttcca
     7501 ccatgtattt tcaggacgta caggctgtac taggatttgg attcaagact taggggtctc
     7561 ctatgtggat ctcctatgct gcctaggtgc tgcctccaga gcctgtctct gcctcatcag
     7621 ctcaaggggc cttccgtttc ctccgtgctc cttgggttgc cctgaagagc acagaatctg
     7681 cctgttcctt tctggtagcc tttccctgtg gccttcttag tctgcagcta tctttgtgat
     7741 cctccttggc ctgtgccttt gaatttcctg gctgttaagt ctctgatcac tcccaggtag
     7801 gttctttgtt catttctgac agatcaatga atattgccct ggggtggcgt gtcctttcca
     7861 ctcagcacag ctgatggctg ccacttggcc acctctgaag gcctcatttc tggctgtatc
     7921 tctagagacc tgtggtgtag gaggggtcct ggtgactcct cctcatgttt cctctccatg
     7981 tttgatgtcc ctgagtgtgt ggcctggact gtgtcctctt tctgtgtgag caaatctgta
     8041 aacaggtggt cctgctttct acagcctcct tcctgagtgt ctgggtccat ccatcaagga
     8101 gatgcacagg aactgtagcg ggagatggtg tggggctgga gtggtaggtc cttagtacag
     8161 ccctagacct tgctcatata gatggttttg gggcaggacc ctggtggctt ctctcagatg
     8221 cacagattca ggccctaacc atcctccagc gctgtgcctg gtttcctgct gcctatgcat
     8281 gcgtatgttg gcagctttct ctgaggtttc tctgagctgt gtggattcac tgctgtccat
     8341 catgtggcgc agaagggtga ggtccttaca ctacttcctg gagcctctag gctctcaggg
     8401 actgtgcctg tcctcttagt tgttgcccgg atggactatg tggactcaga ctttccttga
     8461 aactcaccag ggatgcagct atcttcagtc agaggagtgg gctctcactg tcacagtgtg
     8521 tcacagtcta ggagagactc agagaatggc agcaaggaaa gcatcccatg tgcatgccca
     8581 cagaagttcc cccagagaga ggacggccat gatactagac atgttagggg cctggggaag
     8641 agcctgcagc atatcccagc agaaactgag ccttcttcaa cctcctccgc ccatgccagt
     8701 agaatgttct ttccttttct tgcaatttaa cagccatctt tgtcttcctt tgcaaggcaa
     8761 catgggatgg ggcatgggag gtctgaggga agatgagggc cctgaaactt cagatctggc
     8821 acacagcaac tcaagcctgg caggtcagaa caagccaggg ctgaggtcag ggaaagtcac
     8881 atctggaaga tgtgagaaag gccagctcat actgtccatc cagacaggaa gcaaaggcct
     8941 gggagtgtga agggatgcat tctttggggc atttcagccc ttcttgaaac aaagcaaagc
     9001 aaagcaaagc aaaaacaaga acccgtgctt cttttctctt cttggggaga gaaaaaaatt
     9061 gcttactcta cccaagtcag ggagacacaa ggagttaatg aaaggcgcat aacgatacca
     9121 cgatatcaac aagtttgtac aaaaaagcag gctggcgccg gaaccgaagt tcctattccg
     9181 aagttcctat tctctagaaa gtataggaac ttcgaaccct ttcccacacc accctccaca
     9241 cttgccccaa acactgccaa ctatgtagga ggaaggggtt gggactaaca gaagaacccg
     9301 ttgtggggaa gctgttggga gggtcacttt atgttcttgc ccaaggtcag ttgggtggcc
     9361 tgcttctgat gaggtggtcc caaggtctgg ggtagaaggt gagagggaca ggccaccaag
     9421 gtcagccccc cccccctatc ccataggagc caggtccctc tcctggacag gaagactgaa
     9481 ggggagatgc cagagactca gtgaagcctg gggtacccta ttggagtcct tcaaggaaac
     9541 aaacttggcc tcaccaggcc tcagccttgg ctcctcctgg gaactctact gcccttggga
     9601 tccccttgta gttgtgggtt acataggaag ggggacggga ttccccttga ctggctagcc
     9661 tactcttttc ttcagtcttc tccatctcct ctcacctgtc tctcgaccct ttccctagga
     9721 tagacttgga aaaagataag gggagaaaac aaatgcaaac gaggccagaa agattttggc
     9781 tgggcattcc ttccgctagc ttttattggg atcccctagt ttgtgatagg ccttttagct
     9841 acatctgcca atccatctca ttttcacaca cacacacacc actttccttc tggtcagtgg
     9901 gcacatgtcc agcctcaagt ttatatcacc acccccaatg cccaacactt gtatggcctt
     9961 gggcgggtca tccccccccc cacccccagt atctgcaacc tcaagctagc ttgggtgcgt
    10021 tggttgtgga taagtagcta gactccagca accagtaacc tctgcccttt ctcctccatg
    10081 acaaccaggc tcccaggtcc cgaaaaccaa agaagaagaa cgcagatctc tagagtcacc
    10141 gagttgcttt accggatgaa gagggccgaa acatactgtc caaggccctt gctggcaatc
    10201 cacccaactg aagccagaca caaacagaaa attgtggcac cggtgaaaca gactttgaat
    10261 tttgaccttc tcaagttggc gggagacgtc gagtccaacc ccggaccggt gcatgtggac
    10321 cccaagaaaa agcggaaggt ggaccctaaa aagaaacgca aagtggatcc taagaaaaaa
    10381 agaaaggtgc acgtgtccat ggtgtccaag ggcgaggaac tgttcaccgg cgtggtgccc
    10441 atcctggtgg aactggatgg cgacgtgaac ggccacaagt tcagcgtgtc cggcgagggc
    10501 gaaggcgacg ccacatacgg aaagctgacc ctgaagttca tctgcaccac cggcaagctg
    10561 cccgtgcctt ggcctaccct cgtgaccaca ctgacctacg gcgtgcagtg cttcagcaga
    10621 taccccgacc atatgaagca gcacgacttc ttcaagagcg ccatgcccga gggctacgtg
    10681 caggaaagaa ccatcttctt taaggacgac ggcaactaca agaccagggc cgaagtgaag
    10741 ttcgagggcg acaccctcgt gaacagaatc gagctgaagg gcatcgactt caaagaggac
    10801 ggcaacatcc tgggccacaa gctggagtac aactacaaca gccacaacgt gtacatcatg
    10861 gccgacaagc agaaaaacgg catcaaagtg aacttcaaga tccggcacaa catcgaggac
    10921 ggctccgtgc agctggccga ccactaccag cagaacaccc ctatcggcga cggccctgtg
    10981 ctgctgcctg acaaccacta cctgagcacc cagagcgccc tgagcaagga ccccaacgag
    11041 aagagggacc acatggtgct gctggaattc gtgaccgccg ctggcatcac cctgggcatg
    11101 gacgagctgt acaagcctgc aggcgagggc agaggaagtc ttctaacatg cggtgacgtg
    11161 gaggagaatc ccgggccgtc caatttactg accgtacacc aaaatttgcc tgcattaccg
    11221 gtcgatgcaa cgagtgatga ggttcgcaag aacctgatgg acatgttcag ggatcgccag
    11281 gcgttttctg agcatacctg gaaaatgctt ctgtccgttt gccggtcgtg ggcggcatgg
    11341 tgcaagttga ataaccggaa atggtttccc gcagaacctg aagatgttcg cgattatctt
    11401 ctatatcttc aggcgcgcgg tctggcagta aaaactatcc agcaacattt gggccagcta
    11461 aacatgcttc atcgtcggtc tgggctgcca cgaccaagtg acagcaatgc tgtttcactg
    11521 gttatgcggc ggatccgaaa agaaaacgtt gatgccggtg aacgtgcaaa acaggctcta
    11581 gcgttcgaac gcactgattt cgaccaggtt cgttcactca tggaaaatag cgaccgctgc
    11641 caggatataa ggaatctggc atttctgggg attgcttata acaccctgtt acgtatagcc
    11701 gaaattgcca ggatcagggt taaagatatc tcacgtactg acggtgggag aatgttaatc
    11761 catattggca gaacgaaaac gctggttagc accgcaggtg tagagaaggc acttagcctg
    11821 ggggtaacta aactggtcga gcgatggatt tccgtctctg gtgtagctga tgatccgaat
    11881 aactacctgt tttgccgggt cagaaaaaat ggtgttgccg cgccatctgc caccagccag
    11941 ctatcaactc gcgccctgga agggattttt gaagcaactc atcgattgat ttacggcgct
    12001 aaggatgact ctggtcagag atacctggcc tggtctggac acagtgcccg tgtcggagcc
    12061 gcgcgagata tggcccgcgc tggagtttca ataccggaga tcatgcaagc tggtggctgg
    12121 accaatgtaa atattgtcat gaactatatc cgtaacctgg atagtgaaac aggggcaatg
    12181 gtgcgcctgc tggaagatgg cgatctcgag ccatctgctg gagacatgag agctgccaac
    12241 ctttggccaa gcccgctcat gatcaaacgc tctaagaaga acagcctggc cttgtccctg
    12301 acggccgacc agatggtcag tgccttgttg gatgctgagc cccccatact ctattccgag
    12361 tatgatccta ccagaccctt cagtgaagct tcgatgatgg gcttactgac caacctggca
    12421 gacagggagc tggttcacat gatcaactgg gcgaagaggg tgccaggctt tgtggatttg
    12481 accctccatg atcaggtcca ccttctagaa tgtgcctggc tagagatcct gatgattggt
    12541 ctcgtctggc gctccatgga gcacccagtg aagctactgt ttgctcctaa cttgctcttg
    12601 gacaggaacc agggaaaatg tgtagagggc atggtggaga tcttcgacat gctgctggct
    12661 acatcatctc ggttccgcat gatgaatctg cagggagagg agtttgtgtg cctcaaatct
    12721 attattttgc ttaattctgg agtgtacaca tttctgtcca gcaccctgaa gtctctggaa
    12781 gagaaggacc atatccaccg agtcctggac aagatcacag acactttgat ccacctgatg
    12841 gccaaggcag gcctgaccct gcagcagcag caccagcggc tggcccagct cctcctcatc
    12901 ctctcccaca tcaggcacat gagtaacaaa ggcatggagc atctgtacag catgaagtgc
    12961 aagaacgtgg tgcccctcta tgacctgctg ctggaggcgg cggacgccca ccgcctacat
    13021 gcgcccacta gccgtggagg ggcatccgtg gaggagacgg accaaagcca cttggccact
    13081 gcgggctcta cttcatcgca ttccttgcaa aagtattaca tcacggggga ggcagagggt
    13141 ttccctgcca cagcttgatg aagatctgag ctcttaatta atgatcataa tcagccatat
    13201 cacatctgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
    13261 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
    13321 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
    13381 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccgtttaa actaacttta
    13441 aataattggc attatttaaa gttacatatg gcggccaatt cgccttctgc aggagcgtac
    13501 agaacccagg gccctggcac ccgtgcagac cctggcccac cccacctggg cgctcagtgc
    13561 ccaagagatg tccacaccta ggatgtcccg cggtgggtgg ggggcccgag agacgggcag
    13621 gccgggggca ggcctggcca tgcggggccg aaccgggcac tgcccagcgt gggcgcgggg
    13681 gccacggcgc gcgcccccag cccccgggcc cagcacccca aggcggccaa cgccaaaact
    13741 ctccctcctc ctcttcctca atctcgctct cgctcttttt ttttttcgca aaaggagggg
    13801 agagggggta aaaaaatgct gcactgtgcg gcgaagccgg tgagtgagcg gcgcggggcc
    13861 aatcagcgtg cgccgttccg aaagttgcct tttatggctc gagcggccgc ggcggcgccc
    13921 tataaaaccc agcggcgcga cgcgccacca ccgccgagac cgcgtccgcc ccgcgagcac
    13981 agagcctcgc ctttgccgat cctctagagt cgagatccgc cgccaccatg attgaacaag
    14041 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
    14101 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
    14161 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
    14221 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
    14281 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
    14341 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
    14401 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
    14461 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
    14521 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg
    14581 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
    14641 gattcatcga ctgtggccgg ctgggtgtgg ccgaccgcta tcaggacata gcgttggcta
    14701 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
    14761 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
    14821 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
    14881 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
    14941 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccccgga
    15001 tctaagctct agataagtaa tgatcataat cagccatatc acatctgtag aggttttact
    15061 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
    15121 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
    15181 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
    15241 tgtatcttat catgtctgga tccctaactt taaataatgc caattattta aagttaggaa
    15301 ttaattcacc cagctttctt gtacaaagtg gttgatatct ctatagtcgc agtaggcggg
    15361 aagacagact tattataaac caatcttaag gacaaattgt ggatccagtt agagcagtgg
    15421 aggaggggag agggacctag tggcatcttg gtcagagatg gtggaatttg ctgggctgaa
    15481 gctcagaatg taccataggc cagaagagta gcatgcaagg actgcagagc atgtctgagt
    15541 gcaggccagg ctcacctatg ctggcctagg agtctggagg caattgcttg ctcattagta
    15601 ttccacattt gagtgtcagc acatggggct ctggggactg ggcagtatct gcaggcctgg
    15661 gcatgtgttg gtttgcctga gcaggatcta tagaccctca ctcctctcct aagccagcag
    15721 gaatagggaa gtcctcactg tttgacaaag tatgtctctt ctgtccagga acggttgctc
    15781 atgagcctct tgccccggaa tgtcgccatg gagatgaagg aagacttcct gaagccccct
    15841 gagaggattt ttcacaagat ttacatccag aggcatgaca atgtgaggta ggggaatgac
    15901 agcaacccag ctcaacagtg agtttactga gggggagggg ggctgcaggg tagaggtgac
    15961 aaggttgtga gtgggtagag gagctgggaa gtggatggaa ggagtgagga ggggaattag
    16021 aagatagctc tcgttttgag ggagagagga ggtatggtga aaaagagatg gcgcaacgca
    16081 attaatgata acttcgtata gcatacatta tacgaagtta tggtctgagc tcgccatcag
    16141 ttcatgcatt tcctgatagg gggatagaat gacagttgat agggggacag aactgtgtcc
    16201 caaagactgc gttttttgta gtggtgttta gtgtggggag tctccatttc tgaataatgg
    16261 ctgaacattg cttgagatga gctcttaccc ctcactctcc tcatctcatc atccctagga
    16321 ggtcggagct cttgttccta ttttgcaggc aagggaccct gagtcttccg tatgaaccaa
    16381 cttgtgaata ctttgaagtt tgcttgcctg cttagagagt ctgctgaaac tatttgtcct
    16441 aatgcattgt atagtatttt ttaaaaaata ctttttatta tagttcaatg ctcttagaat
    16501 agttgagtgt cacctgttgg ggagtgccac catgtaaatg ccactcagga gatgactggg
    16561 tggacacaaa gcactttctg ctcagttgtc ttttgctggc tctcctgggt gttaggtgtc
    16621 cggacttcta gccttcagct cctggagaca cagaagcatg ttgaaaaaaa ctcactcatg
    16681 ttggctagca caggctggct catcccaaat ttgttaaggc ttgtgaacca tcacggtaca
    16741 agccatacct gtaagggaga gcagacatgg ctaatcagct cacagctact gctctgtgca
    16801 tatccgtgaa ttcttccact ctctgccatc atgggctttg atagggacca gtgctgtcct
    16861 ccctccacag aggaggatgc agtatgggct tcagacccaa ctgtttggaa aggagccaca
    16921 gagccagccc ttgagcctgg atgctctcca cagagccagc ccttgagcct cgatgctctc
    16981 cacagagcca gcccttgagc ctcgatgctc tccagaatta gggcccagaa gctccttgct
    17041 gagctcatgc ttgtttgctg tcctttcact cccgtgagca gctggcactg ctgcctgcat
    17101 tgtagacatc aggaaattga gactcagcca tgtgaaacaa acacgcagga agccctgggc
    17161 agtgggcaaa gggctggaca caggaccagc tgatgcttgc atttgctgta cgttcctctc
    17221 cgcacctgct gctcctcctt agctggaaag gacacaattc agtgccccaa gggcctgtgg
    17281 aggagcagca gccaccagaa tgtctcagag agaaccggga ggtcgtgttg taggagggtg
    17341 tcatggtgga gggcctcctg gtaactcgtg tgagtaggca tctttggctt ttgcttgctc
    17401 aacaaagcaa acagataaac aaatattaga actagctctt cagcctcctc cctccgtggc
    17461 tttgttaggt ttctgttccc gtgataaaca ctatgactga aagcaaattg gggaggaagg
    17521 ggttaatttg gcttacactt ccacgttgta gtccatcatt gaaggaagtc agggcaggga
    17581 gctgatgcag aggctgcgga gaggtgctgc ttactagtgg cttgctcctc atggcttgct
    17641 cagtctgatt tcttagagaa cctaggccca gcagcccagg agacagcgcc acccacaatg
    17701 gtctgggcct ctccccatca attgctaatt aagaaaatgt cttgcaggct accctacagc
    17761 cctatcttat ggagacatat tattaattga gattccctcc tcacggatgc ctttagcttg
    17821 tgtcaagttg acattaaatt atccagcaca ccctgggagg gagagcactg atcatggtga
    17881 ccaggtcccc agcttctctg gggctgctga aacaaccagc atccgcctct gtcctgactt
    17941 tctgagaggg ctgacttagc ctggcttgct ggggaaatag agtttgggtg tacactggaa
    18001 aacaccacag ttttcacaca catgtcccct gctcaggaca gagtgaacac atggcaccca
    18061 cagctggtta ctggggacac agagcattga taaagggccc gaacatcata gttgaaataa
    18121 gtgatggagt cgctctcttc tgtgaggctg gtctctgaag gtcgtgctga gccaggcaga
    18181 ggcgctcaaa caaaggagga ggagagatct gtagatgcag acctaaggaa atgcatgtaa
    18241 atggatcgct gcacaagagg agagaaataa ctctcaggga gacagagaag aattacgcac
    18301 atgaaagatg agaatttcaa tgaggacata aaagcataaa ggagtaacaa atcagaaggc
    18361 tcacgactgt agcaaccaaa tataaaactc cccacaggag aagagagaat caagcagaac
    18421 aaaagatcgg caaactggaa gaggggtctt cggaagggtc caaaaagaaa gaatggaaag
    18481 aagaaagctt caggcataca ggaagtccgc gctgggacaa cgcacacgtt ataggtgttg
    18541 ctgaagaaga gtgggaaggg caaggtttct ttttttcttt aaagcttttc agacggacat
    18601 gtgaaacaga aatccgcatc cagagtagca aacatatacg acaccaaata agatgaactt
    18661 aaagagattt acatgataaa gttctataca gttagagacc aagggagaac tgtgaaggca
    18721 atagcaggaa agtgttttcc tggaagcaac agaatcccca ttacgtattc agtggatctt
    18781 aggggcaaac catgcagggc agagggagca aatggcgtat tccagatgtc taaaacaaac
    18841 cacaaaaaca ccacaaacca agggtgctgt gcctggcaaa attcttcttc agaaataaag
    18901 gcaaggcaga gattctgtca gacagtgcaa agctgtgggt gcttgtggct accagacctg
    18961 cattatgaaa aaaaaaatgc taaaaaaaag gaaaaaaatg ctaaggggat tctcaggatg
    19021 aaataagact cctctgcagc aacacagggc ataagaaacc atgaaacaca ctgacaaaga
    19081 tgtacatata tgtatgtata tatatatata catatatata catatataca catatacata
    19141 tacacataca tatacatata tatatacata cacacacaca cacacataca tgttatctgt
    19201 gtgtatgaag gtgaatagtt ttttattgtg gttgcgtgat agttaaaagc ctggctagta
    19261 gagatgctct gcggtagagg tgctggatgt gaagcttggt cacctgagtt tgattcccca
    19321 gaacccacaa ggtgggagga gaaaaccaac tctaaccaca acacattcat tatggcatat
    19381 tgtgcaccta tggtatacgt atgtgtttta aaaataactg taatggggaa ataatttaat
    19441 atatgacatg agcacatgta aattgtggtt agtaaattat ggcagggtgt taaaatataa
    19501 atttttttcc ctctgtgact gatcagcaat atgataaata actaattaag gaagttaatt
    19561 gtaagaaaaa aagagaaaat attgaaagca cttcaattca gaaaacccac agatgcagca
    19621 ggacacagat gagaacaaaa acaagattcc caagtctggg aggaaacatg taccccaaag
    19681 gcagtgccag tacaccccat caatacacaa catgtaaatg tcataaaatg tcctaccaat
    19741 caaaaggcag ggtagcaaca tcagccaaac aaacacacat cagacacagt gacaaccagc
    19801 ccgactacat ctgtacaaga agcttgcttc agatgtgagg ctgtaccctg tgctggagga
    19861 gcagagatgg gaaagaacat ccatatgaat gagaatcaag gtggggcagg ggtggccatg
    19921 ccctcatcat cccaggtaca cttatgtcag aactcttaga agacttggca taacgcacag
    19981 ccaactcaaa atgaagtatg ccaagtgagt gtccataaaa ctgaaagggc aaatgtccat
    20041 caaatcagca ttaatgtaat gtacctctga taaatgaata gaacatctag acagaagaac
    20101 agagagagag agaaggaaga ggaggaggag gaggaggagg aggaagagga ggaggaagag
    20161 gagacaatga cgacgatgac aatgaccact ctgtgagcac tatggacctg gtgctaatag
    20221 acacaacaga ccataccact cattcttctg aagtgcttgt ggttctccct agcacaggtt
    20281 atgcaaggtc acagaaaaat cttaatgaat ttgagagaag taaatcatac tgtatttcct
    20341 gacccaatgg caccgtacgt tcagatttca aaccatattg caaagctgtc atgattgaag
    20401 cagcatggtg tgaaagcaga gacttaggat agactagaga aaggagccca gaagtgaacc
    20461 tacatacgtg tactcagcct gtcctcaagg gtggcaaaat ggaataaaga aaggtttatc
    20521 ttcaatagat cgtgctagaa atactgaaca tccacaggtt ggacagtaca attggaccct
    20581 catccgatac aatacgcaga atcaattaaa atcgattcta acttgaaaag tataaaatcc
    20641 tagaaagaga aacccgtgct aagttctctg acattg
//