LOCUS       232916#pL1L2GT0_LF2A_nEGFPOT2A_CreERT2_pAbActNeopA#L3L4_pD223_DTA_T_spec        22607 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2GT0_LF2A_nEGFPOT2A_CreERT2_pAbActNeopA
COMMENT     design_id: 232916
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000108180
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     misc_feature    4275..10026
                     /note="5 arm"
     misc_feature    10054..10078
                     /note="B1 Gateway"
     misc_feature    11045..11048
                     /note="FCHK QC critical region"
     misc_feature    11029..11044
                     /note="FCHK QC prefix region"
     misc_feature    11049..11054
                     /note="FCHK QC suffix region"
     misc_feature    11177..11228
                     /note="F2A"
     misc_feature    11054..11176
                     /note="1Dleader"
     primer          10914..10935
                     /vntifkey="27"
                     /dnas_title="seq primer FCHK2"
                     /label=seq primer FCHK2
                     /note="seq primer FCHK2"
     exon            11014..11053
                     /vntifkey="61"
                     /dnas_title="mouse En2 exon"
                     /label=mouse En2 exon
                     /note="mouse En2 exon"
     misc_feature    12100..12100
                     /note=" A to G to kill RsRII site"
     misc_feature    12050..12103
                     /note="self-cleaving' 2A peptide"
     misc_feature    12104..13129
                     /note="Cre"
     misc_feature    11234..11323
                     /note="3X NLS"
     misc_feature    11324..12040
                     /note="EGFP"
     primer          complement(10207..10228)
                     /vntifkey="27"
                     /dnas_title="LRPCR LAR3_EN2"
                     /label=LRPCR LAR3_EN2
                     /note="LRPCR LAR3_EN2"
     intron          10139..11013
                     /vntifkey="21"
                     /dnas_title="mouse En2 intron"
                     /label=mouse En2 intron
                     /note="mouse En2 intron"
     primer          complement(10103..10126)
                     /vntifkey="27"
                     /dnas_title="FRT3_oligo"
                     /label=FRT3_oligo
                     /note="FRT3"
     misc_feature    10091..10138
                     /vntifkey="21"
                     /dnas_title="Frt"
                     /label=Frt
                     /note="Frt"
     misc_feature    14390..14397
                     /vntifkey="21"
                     /dnas_title="polylinker"
                     /label=polylinker
     misc_recomb     14358..14389
                     /note="Rox(inv)"
     misc_feature    14350..14357
                     /vntifkey="21"
                     /dnas_title="RE sites"
                     /label=RE sites
     primer          14290..14308
                     /vntifkey="27"
                     /dnas_title="seq primer NF"
                     /label=seq primer NF
     primer          14164..14187
                     /vntifkey="27"
                     /dnas_title="LRPCR RAF5"
                     /label=LRPCR RAF5
     misc_feature    14107..14349
                     /vntifkey="21"
                     /dnas_title="SV40 pA"
                     /label=SV40 pA
     misc_feature    13139..14083
                     /note="ERT2"
     misc_feature    15185..15767
                     /note="synthetic fragment mutates RsRII"
     misc_feature    15597..15597
                     /note="G to C conservative mutation to kill RsrII site"
     misc_feature    15945..16188
                     /note="SV40pA"
     misc_feature    16003..16026
                     /note="RAF5"
     misc_feature    15752..15771
                     /note="neoBstB1_5"
     misc_feature    15459..15477
                     /note="neoSphI_5"
     misc_feature    15122..15141
                     /note="neoPstI_5"
     misc_feature    14953..15938
                     /note="neomycin resistance "
     misc_feature    14803..14822
                     /note="betact5"
     promoter        14407..14927
                     /note="human beta actin promoter"
     insert          14104..16183
                     /source="pL1L2_GTIRES_BetactP FLFL MapVerified"
     misc_feature    16184..16188
                     /note="for bActNeo"
     misc_recomb     16190..16221
                     /note="Rox"
     misc_feature    16234..16254
                     /note="B2 Gateway"
     misc_feature    10027..16284
                     /note="Synthetic Cassette"
     exon            16639..18228
                     /db_xref="ENSEMBL:ENSMUSE00000675858"
                     /type="targeted"
                     /rank="5"
                     /note="target exon 1 ENSMUSE00000675858"
     misc_feature    16285..18414
                     /note="Critical Region"
     misc_feature    18438..18471
                     /note="Downstream LoxP"
     misc_feature    18415..18494
                     /note="synthetic loxP region"
     misc_feature    18495..22607
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggaatgaac tgtttattta atagctatgg gttctttgac caccatcttc
     4321 caatctcttc tgccatctag ccatcccatc cgcattttct tttctgtgcc tatgaaatct
     4381 acgctagact gtacatgtga gtgaactact gcaatcgtgc ctctccgtgc ctggctgatt
     4441 tgacttgtac aacttcccat gtgaccatct atgtagttac aaatagcagg gtttgtgcgt
     4501 tttatgctct attgtattct actgtgtgca tatcctgtac ttcctctatc tattatagag
     4561 aattagctac tgagcattgt gctgcagcag taagtacagg gatacaaaca tactgtattt
     4621 atttcctttg gaaacacacc cagcagtggg gtttcatttt ttacttttta gagaattctc
     4681 aatggctcct tttctctgca gcctcaccaa cacatactaa gtttcaccct tctaataaca
     4741 gcaatcctga tgggtttttt ataatttttt gagacaggct ctcactatgt agccctggct
     4801 agcataggta gacttcctta tgtagaccaa gctggtttca aattcacata gatctatctg
     4861 cctgcctctg cttctggagt gctgaggtta agggtcatag ctgctctctt actatttttt
     4921 acatttggtc cttagtaaat gtagaacttg cagacatgtt cccacatctc tttgtactct
     4981 gtaattcctt tgtaattcct taatccctaa gtagaacctg tctttattat gatacatgcc
     5041 ccttggtcta gttttgcttt tgttccttca taccccaaat gaggcagtat atccagtatc
     5101 caaaaccatc atagtccaga ccaacacaat ggaattctgt gcacactccc atgcattctc
     5161 tgtagttcga gatcccatgt ttgagtcttt ctttcataca ctttgagtga atatttgtat
     5221 ctggtgagag acacagatct gttttcatgc tgtgaagtcg tacagttttc ccagtagcac
     5281 ttacagaagt gattttcttt ctctgctgtg tgtttttagc acctttgtca gatcagctgt
     5341 ctgtaaatgt atgggtttat ttctgggctc tctgttccat tctcctggtc tggctctgtg
     5401 tgatgccatg acagtgctgc ttatgggcta atgcagttta tgttatgttt gtaaaggggg
     5461 taagacaatg cttcttgctt tatttttctt gtagatttct ttggttcttc gggatctttt
     5521 gtggttccat actgttgata gaactctctc ccagggagag acataaagca ttgtgtagtc
     5581 ctcctctcag agtcctaatg accaacggaa acgtgaccca cctaaagctc cctctagaga
     5641 gccatgagtt tacagcttcc ttacagagta taagtgagag gtcacttaca gggtacggga
     5701 ctccttcccc aactagctgt acctgataac ccttacccag cagtggtgtg ggcttcctgt
     5761 agccctatct agtatttctt gactgataca taataagtat atatacgtat agtcagagat
     5821 atagagaata tagaatcttt ctacatatac tatatataca tactctttct atttccatat
     5881 atactatatc tctgaatgat acacacacac acacactaac ccctccccta acccacatgc
     5941 agagtcccca gatgggagga agcagttgga tgtagctgca tactccagca agaatcgcct
     6001 tgctatgctg gggcataaac caccaacaag cttagctgag atgaaatatt gcaggggaac
     6061 acagctgaat gccccaagat ggggcagttg cttgacatag aagacagtca ctcacaacac
     6121 attccaaaaa ataataataa taattaagag gcataaccag atttgaaaaa gccagctact
     6181 gccatggaag tcccttaaaa tatataaata tacatatatc aaaagtttaa atggagttac
     6241 cctatgttcc agttagtttc tattgctgtg attaaaacca tgaccaaaaa caacttgggg
     6301 aggaaaaatg tttaacctta caacttaaag ttcctcatga ggggaaatca ggacagaaac
     6361 taaagcagac catgttcaga gatttgttca gcaaccttct ccccaggggt agcaccgccc
     6421 acaaggaggc tgaaccctct cacatcaatc aagaacattc ttcacaaacc tgcctacagg
     6481 ctgaactgat gaggcatttc tttaatcaat gtcccctttt cctaggaaat tcaaccttat
     6541 gtcaagttga caaccacggc atcctataac agggaaatac tactcaaact agatagcaca
     6601 ggctagcaaa tacaaaccct aagagtaggc caccattttt gaggtgttac ccaaacaatg
     6661 atatctattg tcattcagaa cttggtggta ggactctagt gctaatgaca ccacctgctt
     6721 gagtcatgag catggaagtt tgatcaatac tgcctcgctt tcatagtgct aaaaggtgct
     6781 gtgtacacta atggaagaga agagttatca tcagtctcac ctaacagacc ccagagagct
     6841 tcaggaatga cctaagagct acagtaatgg cctgcctggc aactatgtcc actggtacaa
     6901 tagcggcaca gatgttatgg cgataactca ccaccttctg attgtattca agacccaccc
     6961 catgagatgg aactcatact tggcactgtt aatgaaagca agaacttcta gctaaaaatt
     7021 tcacggccct tagagaacaa cctactaata tcagtctaca tctaagcgga atgatattaa
     7081 aatgatgacc cccaataact taaggccatg cctgtagatc agtgcatctc ttcaactctc
     7141 atcagacagg tttctccttg caacagatgg cgattaacac agagacacac agctggtcaa
     7201 catacagaaa aattagagac ttcagagttc caagccctaa atgggacttc tgccactcct
     7261 tccagggatt aaggctcttc atggaacatg gggtgggaag actataagag ccccaagtga
     7321 tggatgactt caaggaggca atgttttcca gacacaagaa gacagtgcac atatgaactc
     7381 acagcattgc taacaacatg aacaaaacct gggcaagacc aaaccagaca aagtccaagc
     7441 atgggacgga gaggtgggca caaagtccta cccctctctg aggaactatt ggcatttgat
     7501 ggcttctcag aggaggagag tcaggcttct gtaatggtca ttaaacggtc aaccatactt
     7561 catagcagcc tcaaaacatc ggacattttt ggtggcagtg gaggtgatac gttttgtttt
     7621 taagagtggg gtggaggaga acaagatatt gactgggtgg gaagttagta ggggagggtc
     7681 tagaatagtg gtctctggct gtgggttaaa atgctttttg ggtcacatca tacatccttc
     7741 atatcagata tttacattat atttcataaa aatagtaaaa ttatagctat gaagtagcaa
     7801 tgaaataatt ttatggacgg agtcagcaca ccatgaggga ctgaatgaaa aggtcgcagc
     7861 atcaggaagg ttgagaatca ctgctctagg aggagttggt ggaggaggtg aatatcatca
     7921 aaatagattt gtgaaattct cagagaatga ataaaaatat tgtttttgta aaaagaggca
     7981 gaaccaggcc atgtgtggtg atgtacagat ataattctag aacttggaag gcagaggaag
     8041 tcagagttta aagacagcct ggatatatag tgaggagaga ggggggggag agggagaggg
     8101 ggagagagag aggggagaga gagagagaga gagagaggga gagagagaga gaagagagag
     8161 agaagagaga gagagggggg gagggagaaa gagaggagag agagggagag agagagaggg
     8221 agagagagag ggagagagag ggagagagag ggagagagag agaaagaaga atgttgccca
     8281 ggatgacctc tatctaattt cagacaaccc tctcagcttt tcaggtagcg agaactataa
     8341 ggcatatgtc accaaaactg taaggtattt ttgacttcta taaataatat cattcatact
     8401 tcagtactgg ttgaatcaga ttgtatatag atttgcctag acaatatgaa tgtattaaca
     8461 atattaattc ttgtagccca cgtatacaga gtatccgtct gtttgtctct atgtctccag
     8521 cttctttctt caatgtttta ttggttttcc ctgtagagat ctttaacctc gttggttaga
     8581 tttgttcctc catgtttcat gtagctctcg aaaatgggat ttccacttca gacaactcac
     8641 tgctagtata taaagtgctg cttggtttcg tatgttgatt tttgtgttta ataagtttat
     8701 tcttagttca tgatcataaa cactggactc ttcagggtta cttgttctta aaaatcatgt
     8761 catctgaaag ttgtaacaat ttaacttctc cctttctggc cttttattta gttcctcctt
     8821 ccatacagga tttctctgtc tttattccaa atgggcttgt tactgatggc tgaatccctc
     8881 tgaactaaca gattgtttat tttatttatt tatttaccta acaggttatt tatatttggt
     8941 ttttatcaag tacttccaca acatcgacat ttaaaatccc attcggtgga gactccagga
     9001 tctttgctct ctctggctcc aagagttttg agagcacagt gggttgaatc ttgtgcagtg
     9061 gagacaggtc tcttaatgag cttgggctgg ctccctgtgc tgcaggaagt ttaagacatg
     9121 cagaagtcag ccacccagct taccgctcca tcttcctccc actccctccc ccgcagaaac
     9181 ttttgatcac tgccactgta gctgcagcaa gcacccaaga cagctgctct tgtttgatgt
     9241 gttcacatga tttgtagatc tgtctccctt cccccggggt cagcagcggg cttcataaga
     9301 atctacggga aatcaaatag tatatgcagg gttaggaagc ctgccctgag acccacagtt
     9361 tagacatttc tatcttttca acaacaacaa caaaggcagc tagacacagg caacatactt
     9421 ttaattgtct aagtcattgt ctttgtgtgt gtctgtgtac acacatatgt ggaagctatg
     9481 catgagtaca tttttcagcc tgtaaaatat actggagaag cagcaagaca tagactgagc
     9541 tgcagaaggt tttagacatc tccatggcaa agctgctgat gataattagg gaaggaatgc
     9601 gtccgtgccc tttactactt ggaggctaca gttattgaag tgacagctga ctgtgtagat
     9661 ctgtacactc tcaccctact ctctttgaac caacagttag tttcttgtaa gcaatcatca
     9721 tcaactttgt cattataaga tcagctatga cacgctaatt aaaaaatccc aagtagggcc
     9781 aggcacagtg gtgcgcatct ttaatcccag cccttgggag agagaagcag gcagatctct
     9841 gttgcatttg agggcagtct agtctacata gtgagtttca ggacagccag gtctacatag
     9901 tgagaccctg tctcaaaaaa aaaaaaaaga aaagaaaaaa gaaaaaaaaa tcccaagtag
     9961 aagctgtgaa ggttgagctg agcaaaagag ggattttcag tgtttctttt aacattgtaa
    10021 tgaaaaaagg cgcataacga taccacgata tcaacaagtt tgtacaaaaa agcaggctgg
    10081 cgccggaacc gaagttccta ttccgaagtt cctattctct agaaagtata ggaacttcga
    10141 accctttccc acaccaccct ccacacttgc cccaaacact gccaactatg taggaggaag
    10201 gggttgggac taacagaaga acccgttgtg gggaagctgt tgggagggtc actttatgtt
    10261 cttgcccaag gtcagttggg tggcctgctt ctgatgaggt ggtcccaagg tctggggtag
    10321 aaggtgagag ggacaggcca ccaaggtcag cccccccccc ctatcccata ggagccaggt
    10381 ccctctcctg gacaggaaga ctgaagggga gatgccagag actcagtgaa gcctggggta
    10441 ccctattgga gtccttcaag gaaacaaact tggcctcacc aggcctcagc cttggctcct
    10501 cctgggaact ctactgccct tgggatcccc ttgtagttgt gggttacata ggaaggggga
    10561 cgggattccc cttgactggc tagcctactc ttttcttcag tcttctccat ctcctctcac
    10621 ctgtctctcg accctttccc taggatagac ttggaaaaag ataaggggag aaaacaaatg
    10681 caaacgaggc cagaaagatt ttggctgggc attccttccg ctagctttta ttgggatccc
    10741 ctagtttgtg ataggccttt tagctacatc tgccaatcca tctcattttc acacacacac
    10801 acaccacttt ccttctggtc agtgggcaca tgtccagcct caagtttata tcaccacccc
    10861 caatgcccaa cacttgtatg gccttgggcg ggtcatcccc ccccccaccc ccagtatctg
    10921 caacctcaag ctagcttggg tgcgttggtt gtggataagt agctagactc cagcaaccag
    10981 taacctctgc cctttctcct ccatgacaac caggctccca ggtcccgaaa accaaagaag
    11041 aagaacgcag atctctagag tcaccgagtt gctttaccgg atgaagaggg ccgaaacata
    11101 ctgtccaagg cccttgctgg caatccaccc aactgaagcc agacacaaac agaaaattgt
    11161 ggcaccggtg aaacagactt tgaattttga ccttctcaag ttggcgggag acgtcgagtc
    11221 caaccccgga ccggtgcatg tggaccccaa gaaaaagcgg aaggtggacc ctaaaaagaa
    11281 acgcaaagtg gatcctaaga aaaaaagaaa ggtgcacgtg tccatggtgt ccaagggcga
    11341 ggaactgttc accggcgtgg tgcccatcct ggtggaactg gatggcgacg tgaacggcca
    11401 caagttcagc gtgtccggcg agggcgaagg cgacgccaca tacggaaagc tgaccctgaa
    11461 gttcatctgc accaccggca agctgcccgt gccttggcct accctcgtga ccacactgac
    11521 ctacggcgtg cagtgcttca gcagataccc cgaccatatg aagcagcacg acttcttcaa
    11581 gagcgccatg cccgagggct acgtgcagga aagaaccatc ttctttaagg acgacggcaa
    11641 ctacaagacc agggccgaag tgaagttcga gggcgacacc ctcgtgaaca gaatcgagct
    11701 gaagggcatc gacttcaaag aggacggcaa catcctgggc cacaagctgg agtacaacta
    11761 caacagccac aacgtgtaca tcatggccga caagcagaaa aacggcatca aagtgaactt
    11821 caagatccgg cacaacatcg aggacggctc cgtgcagctg gccgaccact accagcagaa
    11881 cacccctatc ggcgacggcc ctgtgctgct gcctgacaac cactacctga gcacccagag
    11941 cgccctgagc aaggacccca acgagaagag ggaccacatg gtgctgctgg aattcgtgac
    12001 cgccgctggc atcaccctgg gcatggacga gctgtacaag cctgcaggcg agggcagagg
    12061 aagtcttcta acatgcggtg acgtggagga gaatcccggg ccgtccaatt tactgaccgt
    12121 acaccaaaat ttgcctgcat taccggtcga tgcaacgagt gatgaggttc gcaagaacct
    12181 gatggacatg ttcagggatc gccaggcgtt ttctgagcat acctggaaaa tgcttctgtc
    12241 cgtttgccgg tcgtgggcgg catggtgcaa gttgaataac cggaaatggt ttcccgcaga
    12301 acctgaagat gttcgcgatt atcttctata tcttcaggcg cgcggtctgg cagtaaaaac
    12361 tatccagcaa catttgggcc agctaaacat gcttcatcgt cggtctgggc tgccacgacc
    12421 aagtgacagc aatgctgttt cactggttat gcggcggatc cgaaaagaaa acgttgatgc
    12481 cggtgaacgt gcaaaacagg ctctagcgtt cgaacgcact gatttcgacc aggttcgttc
    12541 actcatggaa aatagcgacc gctgccagga tataaggaat ctggcatttc tggggattgc
    12601 ttataacacc ctgttacgta tagccgaaat tgccaggatc agggttaaag atatctcacg
    12661 tactgacggt gggagaatgt taatccatat tggcagaacg aaaacgctgg ttagcaccgc
    12721 aggtgtagag aaggcactta gcctgggggt aactaaactg gtcgagcgat ggatttccgt
    12781 ctctggtgta gctgatgatc cgaataacta cctgttttgc cgggtcagaa aaaatggtgt
    12841 tgccgcgcca tctgccacca gccagctatc aactcgcgcc ctggaaggga tttttgaagc
    12901 aactcatcga ttgatttacg gcgctaagga tgactctggt cagagatacc tggcctggtc
    12961 tggacacagt gcccgtgtcg gagccgcgcg agatatggcc cgcgctggag tttcaatacc
    13021 ggagatcatg caagctggtg gctggaccaa tgtaaatatt gtcatgaact atatccgtaa
    13081 cctggatagt gaaacagggg caatggtgcg cctgctggaa gatggcgatc tcgagccatc
    13141 tgctggagac atgagagctg ccaacctttg gccaagcccg ctcatgatca aacgctctaa
    13201 gaagaacagc ctggccttgt ccctgacggc cgaccagatg gtcagtgcct tgttggatgc
    13261 tgagcccccc atactctatt ccgagtatga tcctaccaga cccttcagtg aagcttcgat
    13321 gatgggctta ctgaccaacc tggcagacag ggagctggtt cacatgatca actgggcgaa
    13381 gagggtgcca ggctttgtgg atttgaccct ccatgatcag gtccaccttc tagaatgtgc
    13441 ctggctagag atcctgatga ttggtctcgt ctggcgctcc atggagcacc cagtgaagct
    13501 actgtttgct cctaacttgc tcttggacag gaaccaggga aaatgtgtag agggcatggt
    13561 ggagatcttc gacatgctgc tggctacatc atctcggttc cgcatgatga atctgcaggg
    13621 agaggagttt gtgtgcctca aatctattat tttgcttaat tctggagtgt acacatttct
    13681 gtccagcacc ctgaagtctc tggaagagaa ggaccatatc caccgagtcc tggacaagat
    13741 cacagacact ttgatccacc tgatggccaa ggcaggcctg accctgcagc agcagcacca
    13801 gcggctggcc cagctcctcc tcatcctctc ccacatcagg cacatgagta acaaaggcat
    13861 ggagcatctg tacagcatga agtgcaagaa cgtggtgccc ctctatgacc tgctgctgga
    13921 ggcggcggac gcccaccgcc tacatgcgcc cactagccgt ggaggggcat ccgtggagga
    13981 gacggaccaa agccacttgg ccactgcggg ctctacttca tcgcattcct tgcaaaagta
    14041 ttacatcacg ggggaggcag agggtttccc tgccacagct tgatgaagat ctgagctctt
    14101 aattaatgat cataatcagc catatcacat ctgtagaggt tttacttgct ttaaaaaacc
    14161 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
    14221 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
    14281 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
    14341 tctggatccg tttaaactaa ctttaaataa ttggcattat ttaaagttac atatggcggc
    14401 caattcgcct tctgcaggag cgtacagaac ccagggccct ggcacccgtg cagaccctgg
    14461 cccaccccac ctgggcgctc agtgcccaag agatgtccac acctaggatg tcccgcggtg
    14521 ggtggggggc ccgagagacg ggcaggccgg gggcaggcct ggccatgcgg ggccgaaccg
    14581 ggcactgccc agcgtgggcg cgggggccac ggcgcgcgcc cccagccccc gggcccagca
    14641 ccccaaggcg gccaacgcca aaactctccc tcctcctctt cctcaatctc gctctcgctc
    14701 tttttttttt tcgcaaaagg aggggagagg gggtaaaaaa atgctgcact gtgcggcgaa
    14761 gccggtgagt gagcggcgcg gggccaatca gcgtgcgccg ttccgaaagt tgccttttat
    14821 ggctcgagcg gccgcggcgg cgccctataa aacccagcgg cgcgacgcgc caccaccgcc
    14881 gagaccgcgt ccgccccgcg agcacagagc ctcgcctttg ccgatcctct agagtcgaga
    14941 tccgccgcca ccatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
    15001 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
    15061 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
    15121 ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
    15181 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
    15241 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
    15301 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
    15361 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
    15421 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
    15481 cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
    15541 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggccgac
    15601 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
    15661 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
    15721 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag
    15781 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg
    15841 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
    15901 tggagttctt cgcccacccc ccggatctaa gctctagata agtaatgatc ataatcagcc
    15961 atatcacatc tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
    16021 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
    16081 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
    16141 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatccct aactttaaat
    16201 aatgccaatt atttaaagtt aggaattaat tcacccagct ttcttgtaca aagtggttga
    16261 tatctctata gtcgcagtag gcggtggctc tcaacaggac tgacagggac agacatctat
    16321 aggcttccca ttgttctccg catttttgcc aggatatttc attagtgtga acagtgaacc
    16381 ctttgtcatc ttgcctcacc acttgacctt ggcgattctc aattctgctg gtgccgtgaa
    16441 ggacggaggt ggcaggcacg agacaaccac taattattgg aagcagaagc cttgctgacc
    16501 cagtatgaga ttaactgctc ctttgcagtt tttattttag ggcattagca actgtctggt
    16561 tatcttgctt tggttgagaa attcaggaca atagcagtgc tgatggtaac gttggcgatt
    16621 tccctgtttg ttttgcaggt cacggccagg ggctttgggc cactgctaca gtttgcctac
    16681 actgccaagc tgttactcag cagagagaac atccgggagg tcatccgctg tgctgagttc
    16741 ctgcgcatgc acaatctgga ggactcctgc ttcagcttcc tgcagaccca gctcctgaac
    16801 agggaggatg gcctgtttgt ttgcagaaag gacagtgcgt gccagcgccc ccaagaggac
    16861 catgggaatt ctgcaggaga agaggaggaa gaagaggaaa caatggactc agagacagct
    16921 aggatggctt gcgccacaga ccagatgctc ccagacccca tcagctttga ggccactgcc
    16981 atcccagtag cagagaaaga agaagccctg ctccctgagt ctgaggtacc cacagacacc
    17041 aaggaaaact cagaaaaggg tgcattgacc cagtacccta gatataagaa gtaccagctc
    17101 gcgtgtacca agaatgtcta cagtgcaccc tcacacggta cctcagggtt tgcgagcaca
    17161 ttcagtgaag acagtcctgg caatagcctc aagccggggc tccccatggg acagattaaa
    17221 agtgagccac ccagtgagga gaccgaggag gagagcatca ccctctgcct gtccggagat
    17281 gagaccgaca tcaaagacag accaggagat gtcgagatgg accgaaaaca acccagccct
    17341 gcccctaccc ccagtacccc tactggagcc gcctgcctgg acaggtccag gagtgtgtcc
    17401 tcgccttcct gcctgcggtc tctgttcggt ataacgaaag gtgtggagtc cactggcctg
    17461 cccagtacat ctcagcaacc cttagtcagg agctcagcgt gccctttcaa caaggggatc
    17521 tctcagggtg accttaaaac tgattacacc cctttggcag ggaattacgg acagccccac
    17581 gtgggccaga aggatgtgtc caacttcgca atggggtcgc cactcagggg gcccgggccg
    17641 gagactctct gtaagcagga gggagagctg gaccgccgga gcgtcatctt ctccgcaagc
    17701 gcgtgtgacc aaccgaacac tccagtgcat tcatactctg cggtgagcaa tttggacaaa
    17761 gacctttcgg agccggtgcc aaaaagtcta tgggtgggag ctggccagtc cctccccagc
    17821 tcccaggctt actcccacag cggactgatg gctgaccact tgccaggaag gatacggccc
    17881 aacaccagct gcccggtgcc aatcaaagtc tgtcctcgct cacctccgct cgagaccagg
    17941 actaggacct ccagctcctg ctcctcgtat tcctacgcag aggacgggag tgggggctcc
    18001 ccctgcagcc tccctctctg tgagttctcc tcttcgccct gttcccaggg agccagattc
    18061 cttgccacgg aacatcagga accaggcctg atgggagatg gaatgtacaa ccaagtccga
    18121 ccccagatta aatgtgagca gtcttacgga accaattcca gtgacgagtc tggatcattc
    18181 tcggaagcag acagtgagtc gtgtcctgtg caggacaggg gccaggaggt agggaatcct
    18241 aagtttcaag cgtgtgacct actatctcag tcctttccct ctacccaaac atcactctca
    18301 ggtcgttctt ttatgttgtc atagttaggg tttctgttgc tgtgactaaa cgccatgacc
    18361 aaaaaccaat ctgaggagga aagagtttat ctggtttacg cgtgcatgtt tctggagatg
    18421 gcgcaacgca attaatgata acttcgtata gcatacatta tacgaagtta tggtctgagc
    18481 tcgccatcag ttcaagcccg cattctttta gaactaagga gcaccagctc agggatggca
    18541 ccacccacaa tgggatggac cctcccccac caatccctaa tttaagaaat taccctccag
    18601 ccagatctca tggaggcatt ttctcaatta agattccctc cttccagata gctctagttt
    18661 atgtgtccag ctgacatgag atcagccagc acaggtgtca aggttaaagg tgaccataaa
    18721 tggtgtggta actgtgaatg gcctgtctct tccttggcgg caggatgctg cagtgtgagt
    18781 cagaatcaac catgcagcct tagaacgaac tccctttgca tctaacctgg attcctgtgc
    18841 tcagtgagca gaggctggct gaatgcagtg catgtaaggt ccatatactt ttggtctcct
    18901 tcccttttcc tctgctgcca tcatacccct ctcttccacc tactttggaa gagtgccctc
    18961 gagtttgctc tgtatccctt cggttcaaag ggaaatgaag aagctcattc aaattgactg
    19021 attgggactg agtagggcct gacaaacagg tgcctctccc tggccttcta agtatttctt
    19081 gtcaatagaa gactccgggg tgaccttgtg tctgtcacag tgcatgacag aatgctcatc
    19141 atccgtctta gacatgggat ccattcagaa atgtcttgtt ggcacacata actgtgtcag
    19201 ggcttgtttc tttcccaggg tccacaaatg agaagaagtc ctgtatgtgg tggtgagtgg
    19261 ttttatgcag cctgaaagtg ctgtgctttg tagagaaaaa gcttataagg cttcatctgc
    19321 gggtcactgt ttctgggagt ggaaatgggg acagcctgac gacagcagaa gcaagcctca
    19381 catgactatg agaaagagga atgggactcc cagaggtccc tagttcgctg gtaatttgaa
    19441 tgttccctag acaaacctaa gcatgtctag gtcatgacct ggtacccacg gcagtgtctt
    19501 actgattgac gaagaaccac ctaaagaccg agactatgtc tcacacccgt ctcaggagca
    19561 gaagtgagca aacccagtgg cagatcttgc ccctgtgtct gatgaacaca ggaaagagaa
    19621 gcacaggttg ggaatatgac tccatgatat ggggctaacc taccaggacc tggccctgga
    19681 aatccattta aaaaacgaaa tgaggggagg gaagagaggg aggacagaaa gggaggtggc
    19741 tgcccaggag gccagcacgc tcatggttgg tctgctcttc agttatcagc aaagatgaac
    19801 tctaccagtc ggtagaagtg tctcgagggg tgaggagagg tagccacaga gcaggtgtat
    19861 tctgcatcca caagctggtg ggcagaactc gcaagagctt taacttcaga cactgaaaat
    19921 ggactccaga gctgcctcga gctagctgcg gacacaccct gggttcactt cctcttcttt
    19981 gctagtctgc ctggtccctg ctttcctgtt actgtctggc agaaatagag agacagaaaa
    20041 ccccctgaaa agggtttaat ttaagaaaga gaaattgtga aggaagtccg gatgggatct
    20101 gttcctgttt gaatcttacc ccttcactgc gagattgagt tgctatcaat gcccaagaca
    20161 cggcagccct ggtccaacct ttaggagcaa ggcttcctaa aaccaaggat aagtgtgtgt
    20221 tctctctgtt cttccttccc acagccttcc tgaggtttgc cttttgtccc taactgtcct
    20281 taagtgtgcc taatgcagaa aactagtcat tgagtcatga ccatcccaca aatgatcgta
    20341 cttaccaagg actggcagag cacgtgcaga tgcctccgtg tcttccttgg gtctcgaaaa
    20401 gagccttaca cttagcccaa gaaattacct tccttaggaa ctgtcagttt accaacgcca
    20461 tccacatgca tgtctgttgt tcttgttgtt gtttattttt gttttgtttt gttttttctg
    20521 attgggctct gtcaatctcc accttagaac tgaaggctat gttacagggt gaggagtcat
    20581 tcagcctgtg aaacagccac aaacaggatt aatctctcct taaaataccc ttgcatttct
    20641 tttttttttg gggggggggg gggggaagtg gttttttttt gagacagggt ttctctgtat
    20701 tgccctggct gtcctggaac tcactttgta gaccaggctg gcctcgaact cagaaatctg
    20761 cctgcctctg cctcccgagt gctgggatta aaggcatgca ccaccatgcc cagctttgca
    20821 tttcattttt atctttactt ttcaaaacaa acctgttgtt tattcataag cagtaaaaga
    20881 cacatttgcc gaaactcact ctgcccctct tgttagcttg tgttagacaa gaggatgata
    20941 acaaagctga gggtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtggttcc
    21001 gctcccccaa cctcacccca ctccccagaa aaggaattca ctttcccaga agcacccaca
    21061 ccctcagcac acggcccctc tgggagtcag ttacttgttt caagagttac ttggtctggg
    21121 tagacagtct gcctcaaacg tacactgaaa ttaacttgat tacttgaact gatccatttt
    21181 aaaacaccag catctatctg ggattctaca aatactgctc ttaggtgggg tttaaccaag
    21241 taaaccggga aagacaaccg agtctacctc agcccggggc cctctctgaa gcagctacca
    21301 gcaagtacag cagtttctct gcagcttctg tagggagcca aaatgttgga ccagaagaaa
    21361 aacgttagca tatctttcct tctgtaaaat ctatcacttg ttaaatgact ctcctagttc
    21421 ataaaacata gacactccac ccccccatcc tttttcaccc ttccctagct ttgaaaaagc
    21481 ttaaattatc gggttggcga gctggctcag cagttacaag tatgggctgc tgtttcagag
    21541 gacccaggtt cagttcccag cgcccacact catgggtggc tcacagtctt ctgtaaccct
    21601 ggcctcggca aaacactgca catacatggt acaccgacca gacatacaaa taggcagaac
    21661 aactatacac ataaaaaata aatagcatag tgcacgcctt taatcccagc acttgggagg
    21721 cagaggcagg tggatttctg agttcaagac cagcctggtc tacaaagtga gttccaggac
    21781 agccagggct atacagagaa accctttctc aggagaagaa agagagagag agagagagag
    21841 agagagagag agaataaata gatagataga tacatagata gatagataga tagatagcaa
    21901 atttttaaat gaaatataat ttccatgcca taccataaaa tttcccctat tagaatgaaa
    21961 gtttcagtgt ttttagtatt ttcacagtgt taggcaactg tcttccgttt ttggctctag
    22021 cgtattttct ttgacctagg aagagtcctg tccccagtag caggttctcc ccacggcttc
    22081 tttctgtcca ccccaggcaa ccagtaacca cctctgtcct gtactagatg ttttgtataa
    22141 atggtatttg gttttttttc aaaaaggtat cattttcaaa atgtatccct tccatagtta
    22201 cacacaccca tcatatgtag ctccacatgc tgattgctga tacattgtgt ctaggcctgc
    22261 cacattttat tgatccgtca ttgcttgagg gagtttggcg ggtcctacct tctgaatgtt
    22321 tctgaagaat ggtgctatga gcatttgcat gcaagggttt ttaggacggg cttccagttc
    22381 attcactcag tacctctgag tggaactgct gtgtcacgtg gggactccat gtggcctttg
    22441 agggactatt cagccatttc ccaaggcagc ccttccaact ttgcattccc cctatcaggc
    22501 tagttcttct tctcactgct aatacttaac tcttctccgt gtgtctatct gatggatgtg
    22561 acatggcatc tccttaggtt tacaagtttg tttttagatt ttgaaaa
//