LOCUS       209370#pL1L2GT1_LF2A_nEGFPOT2A_CreERT2_pAbActNeopA#L3L4_pD223_DTA_T_spec        22245 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2GT1_LF2A_nEGFPOT2A_CreERT2_pAbActNeopA
COMMENT     design_id: 209370
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000087883
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     misc_feature    4275..10279
                     /note="5 arm"
     misc_feature    10307..10331
                     /note="B1 Gateway"
     misc_feature    11298..11315
                     /note="FCHK QC critical region"
     misc_feature    11282..11297
                     /note="FCHK QC prefix region"
     misc_feature    11316..11321
                     /note="FCHK QC suffix region"
     misc_feature    11444..11495
                     /note="F2A"
     misc_feature    11321..11443
                     /note="1Dleader"
     primer          11167..11188
                     /vntifkey="27"
                     /dnas_title="seq primer FCHK2"
                     /label=seq primer FCHK2
                     /note="seq primer FCHK2"
     exon            11267..11320
                     /vntifkey="61"
                     /dnas_title="mouse En2 exon"
                     /label=mouse En2 exon
                     /note="mouse En2 exon"
     misc_feature    12367..12367
                     /note=" A to G to kill RsRII site"
     misc_feature    12317..12370
                     /note="self-cleaving' 2A peptide"
     misc_feature    12371..13396
                     /note="Cre"
     misc_feature    11501..11590
                     /note="3X NLS"
     misc_feature    11591..12307
                     /note="EGFP"
     primer          complement(10460..10481)
                     /vntifkey="27"
                     /dnas_title="LRPCR LAR3_EN2"
                     /label=LRPCR LAR3_EN2
                     /note="LRPCR LAR3_EN2"
     intron          10392..11266
                     /vntifkey="21"
                     /dnas_title="mouse En2 intron"
                     /label=mouse En2 intron
                     /note="mouse En2 intron"
     primer          complement(10356..10379)
                     /vntifkey="27"
                     /dnas_title="FRT3_oligo"
                     /label=FRT3_oligo
                     /note="FRT3"
     misc_feature    10344..10391
                     /vntifkey="21"
                     /dnas_title="Frt"
                     /label=Frt
                     /note="Frt"
     misc_feature    14657..14664
                     /vntifkey="21"
                     /dnas_title="polylinker"
                     /label=polylinker
     misc_recomb     14625..14656
                     /note="Rox(inv)"
     misc_feature    14617..14624
                     /vntifkey="21"
                     /dnas_title="RE sites"
                     /label=RE sites
     primer          14557..14575
                     /vntifkey="27"
                     /dnas_title="seq primer NF"
                     /label=seq primer NF
     primer          14431..14454
                     /vntifkey="27"
                     /dnas_title="LRPCR RAF5"
                     /label=LRPCR RAF5
     misc_feature    14374..14616
                     /vntifkey="21"
                     /dnas_title="SV40 pA"
                     /label=SV40 pA
     misc_feature    13406..14350
                     /note="ERT2"
     misc_feature    15864..15864
                     /note="G to C conservative mutation to kill RsrII site"
     misc_feature    16212..16455
                     /note="SV40pA"
     misc_feature    16270..16293
                     /note="RAF5"
     misc_feature    16019..16038
                     /note="neoBstB1_5"
     misc_feature    15726..15744
                     /note="neoSphI_5"
     misc_feature    15389..15408
                     /note="neoPstI_5"
     misc_feature    15220..16205
                     /note="neomycin resistance "
     misc_feature    15070..15089
                     /note="betact5"
     promoter        14674..15194
                     /note="human beta actin promoter"
     insert          14371..16450
                     /source="pL1L2_GTIRES_BetactP FLFL MapVerified"
     misc_feature    16451..16455
                     /note="for bActNeo"
     misc_recomb     16457..16488
                     /note="Rox"
     misc_feature    16501..16521
                     /note="B2 Gateway"
     misc_feature    10280..16551
                     /note="Synthetic Cassette"
     exon            16920..17119
                     /db_xref="ENSEMBL:ENSMUSE00001308813"
                     /type="targeted"
                     /rank="2"
                     /note="target exon 1 ENSMUSE00001308813"
     exon            17365..17415
                     /db_xref="ENSEMBL:ENSMUSE00001238656"
                     /type="targeted"
                     /rank="3"
                     /note="target exon 2 ENSMUSE00001238656"
     exon            17829..17942
                     /db_xref="ENSEMBL:ENSMUSE00000700263"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 3 ENSMUSE00000700263"
     misc_feature    16552..18063
                     /note="Critical Region"
     misc_feature    18087..18120
                     /note="Downstream LoxP"
     misc_feature    18064..18143
                     /note="synthetic loxP region"
     exon            19194..19268
                     /db_xref="ENSEMBL:ENSMUSE00000700261"
                     /rank="5"
                     /note="ENSMUSE00000700261"
     exon            20831..20884
                     /db_xref="ENSEMBL:ENSMUSE00000700256"
                     /rank="6"
                     /note="ENSMUSE00000700256"
     exon            21276..21329
                     /db_xref="ENSEMBL:ENSMUSE00000700252"
                     /rank="7"
                     /note="ENSMUSE00000700252"
     exon            22042..22095
                     /db_xref="ENSEMBL:ENSMUSE00000700249"
                     /rank="8"
                     /note="ENSMUSE00000700249"
     misc_feature    18144..22245
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggatccgaa ttactaatca catagaacca atggaataaa atgtattaca
     4321 aagtcacggt agtaacctta cttctaacag aagaaaatac acaattatgt acatatttaa
     4381 atatcagtca tgtaacctac agtgtgtttg taaagcttaa aaacaggaat ggatgaatag
     4441 tttagtactg aaactaaaat ttaatgaaat actacatgat ttgagaacaa aagcataaac
     4501 cttgaacatc tgcctttttt tacttttgta agtatttgta aataatagag attccaagtt
     4561 ccataccatt tttgaaaatt tatttataaa gttttttttt aatggtagtc cttttttcta
     4621 ttccttactc aatttttaac aaattaatct ggtttattaa gcaaaaggct caggagctaa
     4681 tgacttaacc agaaattatc attctttctg taaaaatgta cattgtaagt aaataaagta
     4741 ttaccaatac aagattttgt tgtttctatt cataaccccc ttttatttcc tcagcatctc
     4801 aacagtactc agaatgatgg gtaaagggcc tataacagac cctgcatcac atttagatct
     4861 aggatggacc agtcaagcat tgtagaagag agcaagtctt cggaaaccat gctgggaggg
     4921 agaagtggag aggaaaggcc cattaatgag tgcagattct tcggtgactt gcctttggat
     4981 ttctttttta aaagatttgt ccagaactat aggagatgct aaaagaaagc aaggcctcat
     5041 tagaaaggcc tgtttcatat ctacaggctc agaccaggcc tggagtggta actcagaaaa
     5101 gactgccaac cactgaagct ctgtggtgac cactgaggat gactgtccct agtgccgggt
     5161 tcctatgtgt actttgtgac aggcaataac tgataacgcc accttgaagc cattaaagtg
     5221 attgcttgta agtcagagcc gggaagatca aagatgagct tgcctttgct ggacctggca
     5281 gacactaaag cttcgggtac actgtgactt tctggcaatg tgtgtgagct aattctcata
     5341 aaatgtttcg ttctgtttct tagagctaaa agcttagtgt cattattttc catgcttagg
     5401 aaaaaaaaaa atccacattg tttttttttt taatccaaaa gttttaaagg aggaaacttt
     5461 gcctgtcagg aaagcagtgg aaactctgag ctagctctgc acagcagtcg tttgttagct
     5521 gcactttgga gagttacatt aaccactaga agttctgtct gtgcctaaca aaaacatagc
     5581 ccaagtggat tcctttcaga ttaactaatc aaagccttgc tttttccatg gctgaggtca
     5641 agccctgcca tttcaaatgt aatctcaatg aacaaaacgg aaagttcaaa tggaagaaat
     5701 actaaaattg ccccaaagaa gaaattgcca caactaatcc aaaacttatt aatagttaaa
     5761 ttagctttga taattagcat tgaaggatga ctttctcagt taacatagtt gtgagacatt
     5821 ttttgttctt atgtttgtta ttgtagaggc caacaagcta tgtttctcac agaaacactt
     5881 tatgatgttg cacagaattt atagtgccca agtagcattc aaaggagact tgtgctcaag
     5941 ttagaagtta acaaatgcaa atcaagcaaa aactatagtt tttgaatagt agtgccttag
     6001 ccatcctaaa tacatctatg tatctgatga agaatgttta tgtttgtttt tgtcagtctt
     6061 agtgcctggc ttccaagtca cacattcatc ttgctggtgg tctggatccc atcagggtgg
     6121 ccttggaaat acaagccttt ttcataaggt caagagaaat aaacagtagg attccactcc
     6181 agatggtatc ttcccatttt aagattagag aaaaaaaaga cattttggag atcactgtta
     6241 ttccttaaaa aaaaaaaaat ctgtctgcat actcgagagg gtacagagtt cacaaaccat
     6301 ctgggacagg gcactgaaac aggtctcttt cattctatag tatccttgaa agcagtaaaa
     6361 acataatgga gttaggagta tcagatttta gaaaagcata ctagattcaa atctgaattc
     6421 ctttaagtat tttacctgga taattactca gtttgaccca gattctttgt gtgcattcag
     6481 gattgtgaca gcattcagct caaaacatgc aggaaggaga tcataaaatt gatataaacc
     6541 atcaggtata gtgatgaaca cctgcattcc cagcagtcga atgcagtgat acacaacggc
     6601 atttcgagca gtttgatatt agtgatgcat acctgcattg ctagccgtct gcacctatgt
     6661 tcccagcaat ctgaaacctt ggagcaagga ctgtggtttc aaggctagcc tggtggcatc
     6721 tataatttga aagaaaagaa aaagatgtca gccactcaag agtagtacct agaacagagg
     6781 cttgatctat cattcgtttg ccaagtactg tcaactacca tgacaccgtg aaaagaatta
     6841 aagtatcatg taatgatagg aaagattaca tgaacatctg cattctcaaa gtataaaact
     6901 aaagttgcta cacagtaaat taaatatatt aaatattttc ataatttggt aggatccatt
     6961 atagatgtat ttcgatgatt atacatactt tacttttcca tttatctatt tttattttgg
     7021 caaaataaat atcaatcaag ttgtaggaaa agtttaggga cataaaaagc atgtgcctat
     7081 acagaataaa acaaagaaac acctaagcat cattcattta gttttcttgg tggaaagtag
     7141 aataaccaga atctacctag ccatgtaaaa caacacttaa aaatgcactt catttctatt
     7201 tcaaaagact gtctcttcag catctctgtg ctgatgctag aaaggtggct caggagtgca
     7261 gagtgcgtcc tgcacttgca gaggtcccaa ggtcagcttt gagcatctta ctttattacg
     7321 ttgtccagaa ttgaaaatgc aattgtattt atttaatatt tcttacaaaa gtattcagct
     7381 tcctctttat caaagtttct gtgaacacag caccctaata agtgaaaggg acactgtgag
     7441 gtaacttagt cccgcatggc aggtgttatc ctatctttaa atgctgcaaa gcatagagca
     7501 ttccaggaaa tgtcctcttt ccctgcttta caataacttc ccaagttaga tactgaagct
     7561 ggagtcatga ggtacatata tcataggctc agcttctggt atcctacatt ggtaacagta
     7621 gattgccttg tatgaggtga tgaatcagaa gaagttgtgc aacgccccct ttttaggcaa
     7681 agatgactaa gataaatagg aagtgctagc tttcagatgc aagatctact tgtgactttt
     7741 cagaatctga tgtttcaagc atgccatctt tgtttcaagg ggacatgaag taagtctggg
     7801 caacaaatta gattcattat ttcttatttt ttagacattc caagtttatt tatgtagaac
     7861 aacacaactt tctaaagcaa aagaccatat gcaatatcat tgtaaatgtt ctgagcagat
     7921 taaaagaaag aactcacccc aaaaattgag gttacattag gattacaatg gtggtctcta
     7981 gaggcaaagg gcaggagaag aatcgaggat tgcgcagaat gggtgccaat gagaattgac
     8041 cattagataa caccaaaaat attctggtgt agaactacac agtaggacaa cttctagcaa
     8101 taaaagtgtg tagtgtattt taaaggctag gtggaagtat tttcaaaggt tttaccataa
     8161 attacaaatt tttgaggaga taaatatgct taatctggat taaacataat attggtttat
     8221 atgtgtcaat ataatacatg agactccatt aaggtgtagg gtttttacaa cttgatatat
     8281 taattaaagt gaagtaaaaa tgtttttaaa atataagaag taaagatgta ctaagatcca
     8341 ctgaccatta tgttgaaatc tctacacttt aaaaaccact caataagatt tctaagaaaa
     8401 acttagaaac aggaagccaa ataacagttt tctatcatgt aaccctgggt ttaacatatt
     8461 catttaagct tttaatgagt cagcgtaaac attccaaaca aataacaaac actgtctttc
     8521 ttctgatgtg agaatacctt caatgaggta tacacttgtg aagaagaatg catgagaaaa
     8581 acgtttcatg gtatttctaa ggtcatataa aatatatgat gttttttaaa atatgaggaa
     8641 tttaaatcta ttttaattat attttaaata aaataataat tttaataatt attataatga
     8701 aataataaaa tgattttaaa tttattttaa tgtattttat tgtagtttta ctttttaaag
     8761 tatccattag ttccttttaa taacctgcac actttattaa atgtggcaaa atatttctca
     8821 tgcaaaaata aatttttgaa agaatcatta gctcaattta ctagtatttt aagagttgag
     8881 atcaggcagt ttgatgtcca catgttcatg tggaatatcc taaattgaag ctaatcaaat
     8941 gagtatgtgt cagtctaata cagagcatca ttttttattc acattaaaga aggaggaccc
     9001 atgagaagat tttagtgttt tcactgataa tcttttattt cagcacttta atttcatttt
     9061 ttcttaaata caaaatatca gttctaggta aatatacatg gggttaggac ttagcttttt
     9121 tctttaaaac agcaccccag acagccctgc atcaattact ctgaggtatt cagtttggta
     9181 tttagctttt acaatcatta agaatgaagt tgtggtcttt aaagccttag gaatatctgt
     9241 cctgttctca cagtctgtta cagacatcag aggaggatct aagacaactt tgctatctaa
     9301 cacagtttgg ttagtttgtc ttggtttggt ttggtttggt ttggtttggt ttggtttggt
     9361 ttggtttggt ttggtttgac tgtattgtct ttgttactta ttgaaaatgt ctaaactgtc
     9421 tttgactggc tgttaaatca gtacattgtt aagataaaaa ataaatctta cttcatacaa
     9481 gaagcttgag ctatttaatg acatgattaa agtagtattg gcatacatac ataaaagaga
     9541 attagaagca actctgtagt ctatacagac tggatgttct gaaatttgtc ttattgtatt
     9601 taagatttag attattaatg gttttagatt actcaaagta tctaaatatg aagataggga
     9661 attagaaatt ttaaattata attgggctta tgagaaaata aagttttact ctctgagaaa
     9721 atgtaaaaga actcagaaag gcattgctta tattcaaaat tatttgcaga tgtttgaaaa
     9781 cttattattt attcattcat tattggcaaa ctctcatagc aattttcaag ctgcatttaa
     9841 ccatactaca cagttaacat ttcaagaatt caggccaggt tttattttgg catgacttaa
     9901 gttgattcac tgtaaatgac tgctgaaatt tcaatttctt atatataatt aagaaccaag
     9961 tctaaaattt tggttgaagg tatttctctt gccatagtaa gccacaaata cacaaaccat
    10021 cattcgtact tcccctatta tttttactta ttcattgtca atatttgttt taaagtatca
    10081 ctttattttg gggcatttgg aaaactcttt tgttcttgta tgatatacaa cacattatgt
    10141 gatataatac tgacccacta atgtccaaca gagtgagcag tgacattcaa cacaaatcag
    10201 ctttgttagt tctcccagga caatatggaa gaggtttata ctgccaagat tagctcaatt
    10261 tttccatgga tttttgaaga aggcgcataa cgataccacg atatcaacaa gtttgtacaa
    10321 aaaagcaggc tggcgccgga accgaagttc ctattccgaa gttcctattc tctagaaagt
    10381 ataggaactt cgaacccttt cccacaccac cctccacact tgccccaaac actgccaact
    10441 atgtaggagg aaggggttgg gactaacaga agaacccgtt gtggggaagc tgttgggagg
    10501 gtcactttat gttcttgccc aaggtcagtt gggtggcctg cttctgatga ggtggtccca
    10561 aggtctgggg tagaaggtga gagggacagg ccaccaaggt cagccccccc cccctatccc
    10621 ataggagcca ggtccctctc ctggacagga agactgaagg ggagatgcca gagactcagt
    10681 gaagcctggg gtaccctatt ggagtccttc aaggaaacaa acttggcctc accaggcctc
    10741 agccttggct cctcctggga actctactgc ccttgggatc cccttgtagt tgtgggttac
    10801 ataggaaggg ggacgggatt ccccttgact ggctagccta ctcttttctt cagtcttctc
    10861 catctcctct cacctgtctc tcgacccttt ccctaggata gacttggaaa aagataaggg
    10921 gagaaaacaa atgcaaacga ggccagaaag attttggctg ggcattcctt ccgctagctt
    10981 ttattgggat cccctagttt gtgataggcc ttttagctac atctgccaat ccatctcatt
    11041 ttcacacaca cacacaccac tttccttctg gtcagtgggc acatgtccag cctcaagttt
    11101 atatcaccac ccccaatgcc caacacttgt atggccttgg gcgggtcatc ccccccccca
    11161 cccccagtat ctgcaacctc aagctagctt gggtgcgttg gttgtggata agtagctaga
    11221 ctccagcaac cagtaacctc tgccctttct cctccatgac aaccaggctc ccaggtcccg
    11281 aaaaccaaag aagaagaacg cagatcgcag atcgcagatc tctagagtca ccgagttgct
    11341 ttaccggatg aagagggccg aaacatactg tccaaggccc ttgctggcaa tccacccaac
    11401 tgaagccaga cacaaacaga aaattgtggc accggtgaaa cagactttga attttgacct
    11461 tctcaagttg gcgggagacg tcgagtccaa ccccggaccg gtgcatgtgg accccaagaa
    11521 aaagcggaag gtggacccta aaaagaaacg caaagtggat cctaagaaaa aaagaaaggt
    11581 gcacgtgtcc atggtgtcca agggcgagga actgttcacc ggcgtggtgc ccatcctggt
    11641 ggaactggat ggcgacgtga acggccacaa gttcagcgtg tccggcgagg gcgaaggcga
    11701 cgccacatac ggaaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
    11761 ttggcctacc ctcgtgacca cactgaccta cggcgtgcag tgcttcagca gataccccga
    11821 ccatatgaag cagcacgact tcttcaagag cgccatgccc gagggctacg tgcaggaaag
    11881 aaccatcttc tttaaggacg acggcaacta caagaccagg gccgaagtga agttcgaggg
    11941 cgacaccctc gtgaacagaa tcgagctgaa gggcatcgac ttcaaagagg acggcaacat
    12001 cctgggccac aagctggagt acaactacaa cagccacaac gtgtacatca tggccgacaa
    12061 gcagaaaaac ggcatcaaag tgaacttcaa gatccggcac aacatcgagg acggctccgt
    12121 gcagctggcc gaccactacc agcagaacac ccctatcggc gacggccctg tgctgctgcc
    12181 tgacaaccac tacctgagca cccagagcgc cctgagcaag gaccccaacg agaagaggga
    12241 ccacatggtg ctgctggaat tcgtgaccgc cgctggcatc accctgggca tggacgagct
    12301 gtacaagcct gcaggcgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa
    12361 tcccgggccg tccaatttac tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc
    12421 aacgagtgat gaggttcgca agaacctgat ggacatgttc agggatcgcc aggcgttttc
    12481 tgagcatacc tggaaaatgc ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt
    12541 gaataaccgg aaatggtttc ccgcagaacc tgaagatgtt cgcgattatc ttctatatct
    12601 tcaggcgcgc ggtctggcag taaaaactat ccagcaacat ttgggccagc taaacatgct
    12661 tcatcgtcgg tctgggctgc cacgaccaag tgacagcaat gctgtttcac tggttatgcg
    12721 gcggatccga aaagaaaacg ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga
    12781 acgcactgat ttcgaccagg ttcgttcact catggaaaat agcgaccgct gccaggatat
    12841 aaggaatctg gcatttctgg ggattgctta taacaccctg ttacgtatag ccgaaattgc
    12901 caggatcagg gttaaagata tctcacgtac tgacggtggg agaatgttaa tccatattgg
    12961 cagaacgaaa acgctggtta gcaccgcagg tgtagagaag gcacttagcc tgggggtaac
    13021 taaactggtc gagcgatgga tttccgtctc tggtgtagct gatgatccga ataactacct
    13081 gttttgccgg gtcagaaaaa atggtgttgc cgcgccatct gccaccagcc agctatcaac
    13141 tcgcgccctg gaagggattt ttgaagcaac tcatcgattg atttacggcg ctaaggatga
    13201 ctctggtcag agatacctgg cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga
    13261 tatggcccgc gctggagttt caataccgga gatcatgcaa gctggtggct ggaccaatgt
    13321 aaatattgtc atgaactata tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct
    13381 gctggaagat ggcgatctcg agccatctgc tggagacatg agagctgcca acctttggcc
    13441 aagcccgctc atgatcaaac gctctaagaa gaacagcctg gccttgtccc tgacggccga
    13501 ccagatggtc agtgccttgt tggatgctga gccccccata ctctattccg agtatgatcc
    13561 taccagaccc ttcagtgaag cttcgatgat gggcttactg accaacctgg cagacaggga
    13621 gctggttcac atgatcaact gggcgaagag ggtgccaggc tttgtggatt tgaccctcca
    13681 tgatcaggtc caccttctag aatgtgcctg gctagagatc ctgatgattg gtctcgtctg
    13741 gcgctccatg gagcacccag tgaagctact gtttgctcct aacttgctct tggacaggaa
    13801 ccagggaaaa tgtgtagagg gcatggtgga gatcttcgac atgctgctgg ctacatcatc
    13861 tcggttccgc atgatgaatc tgcagggaga ggagtttgtg tgcctcaaat ctattatttt
    13921 gcttaattct ggagtgtaca catttctgtc cagcaccctg aagtctctgg aagagaagga
    13981 ccatatccac cgagtcctgg acaagatcac agacactttg atccacctga tggccaaggc
    14041 aggcctgacc ctgcagcagc agcaccagcg gctggcccag ctcctcctca tcctctccca
    14101 catcaggcac atgagtaaca aaggcatgga gcatctgtac agcatgaagt gcaagaacgt
    14161 ggtgcccctc tatgacctgc tgctggaggc ggcggacgcc caccgcctac atgcgcccac
    14221 tagccgtgga ggggcatccg tggaggagac ggaccaaagc cacttggcca ctgcgggctc
    14281 tacttcatcg cattccttgc aaaagtatta catcacgggg gaggcagagg gtttccctgc
    14341 cacagcttga tgaagatctg agctcttaat taatgatcat aatcagccat atcacatctg
    14401 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
    14461 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
    14521 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
    14581 ccaaactcat caatgtatct tatcatgtct ggatccgttt aaactaactt taaataattg
    14641 gcattattta aagttacata tggcggccaa ttcgccttct gcaggagcgt acagaaccca
    14701 gggccctggc acccgtgcag accctggccc accccacctg ggcgctcagt gcccaagaga
    14761 tgtccacacc taggatgtcc cgcggtgggt ggggggcccg agagacgggc aggccggggg
    14821 caggcctggc catgcggggc cgaaccgggc actgcccagc gtgggcgcgg gggccacggc
    14881 gcgcgccccc agcccccggg cccagcaccc caaggcggcc aacgccaaaa ctctccctcc
    14941 tcctcttcct caatctcgct ctcgctcttt ttttttttcg caaaaggagg ggagaggggg
    15001 taaaaaaatg ctgcactgtg cggcgaagcc ggtgagtgag cggcgcgggg ccaatcagcg
    15061 tgcgccgttc cgaaagttgc cttttatggc tcgagcggcc gcggcggcgc cctataaaac
    15121 ccagcggcgc gacgcgccac caccgccgag accgcgtccg ccccgcgagc acagagcctc
    15181 gcctttgccg atcctctaga gtcgagatcc gccgccacca tgattgaaca agatggattg
    15241 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
    15301 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
    15361 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta
    15421 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
    15481 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
    15541 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
    15601 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
    15661 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
    15721 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc
    15781 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
    15841 gactgtggcc ggctgggtgt ggccgaccgc tatcaggaca tagcgttggc tacccgtgat
    15901 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
    15961 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
    16021 ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt
    16081 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
    16141 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccccg gatctaagct
    16201 ctagataagt aatgatcata atcagccata tcacatctgt agaggtttta cttgctttaa
    16261 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
    16321 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
    16381 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
    16441 atcatgtctg gatccctaac tttaaataat gccaattatt taaagttagg aattaattca
    16501 cccagctttc ttgtacaaag tggttgatat ctctatagtc gcagtaggcg gcccatatgt
    16561 tattagaaca tagaagagca attgggtaaa actgttcatt tccagctgca taagtaatag
    16621 acaagagttt gaatgttatc tactggtgtt aagcttttta atgtaaatga agtaaaaaca
    16681 attgatttga tgaaaaattg cactgtgcca attttatgat aacaagtagt tccttggtac
    16741 atagaaaata tagaggggaa acattatcta ttctgaatga aagaagagca tttcttgttc
    16801 caaactttct tcactttaac tctttttgta aatagtcatt actgcacaac caaaaccaaa
    16861 tgtacatttt gtcaaaacat ttcttaaatt gccatatctt ctcatttatc ttcccacaga
    16921 tgaattggga tgcagccacc ttggtcagtc ctatgagtct agagatgtct ggaagccaga
    16981 accatgtcaa atatgtgtct gtgactcagg atctgtcctt tgcgatgaca taatctgtga
    17041 tgaggagcca ctagactgcc ccaacccaga gatcccattt ggagaatgtt gtgcaatatg
    17101 cccacagcct tctacacctg tgagtctgta gaatatataa actcttcaaa atctacatct
    17161 tcaagaacca tatttaaaca tgtaaatagt ctctactgca tagaggtctg aaagaaatgc
    17221 aagccatgtc tgttgccatg acaagtgaat ggtgtcactt ttaacagaaa gcaggagatt
    17281 gggtataatc agatttaaga aataaagatg taaattgtgc tacagtttac atatattaat
    17341 ctctatattc tttatatgtt ataggctcct gtgcttcctg atggccatgg acctcaaggc
    17401 cccaagggag atccggtaag taacatctcc ccaaggacaa tagttgctaa cgacatttct
    17461 agaaaaggct ggttttcctt gttccaactg aattttcatc attctgaatt ttcttctgtc
    17521 ctaagtatat gaaaatgatg gcaaactcat actgaatgtc tataactgat cacatatgta
    17581 aaggttcagt tttcccaagt ttgaaagtct tcaacccttt ttccatttta aagagaaaat
    17641 gtaggtcatg atataaagga aaacgataaa ccattattca taagacttag tttgaaatat
    17701 acttatgcct aaggacagat tattgtactg ataactagtt tggtaaagca tagaaaaatt
    17761 cctaataatg attctgaacc acttcatgtt ttctctgttt tgttgttttg ttttccttgt
    17821 tttgttaggg tcctcctggc attcctggga gaaatggtga ccctggcctt ccaggacaac
    17881 caggtctccc tgggcctcct ggctcccctg gaatctgtga atcatgtcca actggtggcc
    17941 aagtaagaaa ttacagaatt aaattctctt ctatataagt gcctgagttt gtcattgttt
    18001 ggtctatatt tttatctcat aatggcaact tctgtcctct agttttctat atttataaaa
    18061 cccgagatgg cgcaacgcaa ttaatgataa cttcgtatag catacattat acgaagttat
    18121 ggtctgagct cgccatcagt tcatattcaa ctgtcatcat gtaatacaat tgctactata
    18181 tccttctaaa gtttcccaat ttctcaacat gtccacatta cactcactag gtttgataaa
    18241 cagactatta gctcctaaaa tagttatctt agagtattta ggaagtgcga taattacagg
    18301 caattcccta atcttatcca agcacaaatg gcaaagactt acaaggctgg gcactaaaca
    18361 gctgctccaa aaactgccta ttgaattcat ataaacagag ttgcccgtgc ttctgtgcag
    18421 cctttaatgc acatcttttt cccttctgat ttagggcaac caatcagtct aggaataaaa
    18481 taaataacta agaacataaa cattgtaaag ttctgctgta tgggtttaca cgaacttata
    18541 aaactgtaca aaccaatatg tatgatttat aaaaggtatt ttccaaaaca attcagcagc
    18601 aaggagcatc ctagaaatca aatagagccg agctagccct agaactcaaa gcaagctctg
    18661 agcctttctg atttgtttta ttgtttacaa tgattcatga ctgaagttca gcttttattt
    18721 tattaaatca taggcatggc atatatataa tatatatata tatataattt tattgacttc
    18781 ttgaaaaata attttaatac ccatttttcg tcagatatta gaaacttgga ttttgttgct
    18841 tcaaagttat tttgtcctca aatattggag gattctgtgt atagtgctat aacaattgtg
    18901 tagaaataat ggtttcagta ataacatttg agaccaaatt gaagaggaca gttttgttta
    18961 atatctaaag ttaaaatctc ctccatacat tacacaagca agcatttcta tagtgtgtct
    19021 aatatttaaa gccataaaat gaaacaataa gcaagatata catatcatat atgacatgca
    19081 gaaatgtcag tgaacagtac atgttacaca cactaactac aaatgctttg gaaaataact
    19141 atgatctttg tattaatgtg atctgatcta tgtacttaac ccttttgcta cagaattatt
    19201 ctccccaatt cgactcatat gatgtcaagt ctggagtggg aggaatgggt ggctatccgg
    19261 gaccagctgt aagtacaaat gtttctgagt gttttcaagt atcacactgc ctttgtttct
    19321 tctagaaata caatgttggc atgcaattgt acaaaaagat tcattgacta ctaaggttgg
    19381 aagagaaaac agcaacctgt ccatgatttt tctatcctaa ctcattccct ccctgtggcg
    19441 ttttatgtca ttccatattg ctgcttccta atgcccacct gtactagggc tttttccacc
    19501 accatgctct ggtccgttct tttttcatct aatgtgtaac taaatgtagt tttgttaggg
    19561 attgcttcta ctttacatcc tctcacccaa gttgcatctt ttttcatctt gttcactgat
    19621 ttttacattc taaatgaagt ttaagtcatg aatgtcctat atcacagtct gtttttcatt
    19681 cgggttttaa atccagcatt agcatcttta gaatgcattg atcttttatg ttccactacc
    19741 ataaacaata cctcaacctt gacagtaatg ggctttcaaa actttctaaa tgaccgagaa
    19801 ttttagtaac agaaaagagc aaaaagtttg aaaactggat ttgaagtcac cgatgttggt
    19861 gacttttgct ttggaatagc tctcaggaag tacaacaata ttgaacccta taattcagag
    19921 aaagatcagc ataaataaat acagtatagc atatatttgt caaggtaaag aaaaaatttt
    19981 tatcagtaaa tccaaagtac ctacaaaaga gtactgtatt tggtataaaa gaaattgaaa
    20041 tatatatttc ttctacagtt tttgatatga tttttaaaac tatttgaaaa tcaaatggag
    20101 attatattat atttgtaaat attggttatt attacactca tgttataaat cctcccttaa
    20161 aagattaaca aaaccaaata aaactacaat ttattgacat ttcagagaga taacctaaag
    20221 ttaagttaaa attttgttat cagaatataa aaatagtata aatctgtttt tctagtttgg
    20281 tgtgaatttc tgaataatgt ctagataaat ttgttttaag ttgtgagtgc cccatgcata
    20341 atgattcatg tttatatcta aatcttcaca tcaacattac tgtggtatat catgatagct
    20401 atgaggtttg gagttgactg acatggttat tgtagaaact tataccataa tatctatagt
    20461 atgaagatat ttccacaata tgcttatcca aaaaaattta taacctagag aggtatctat
    20521 gaaatattat aaagcttatc atcacatttt atactgactt tctctgaatt ccctttctta
    20581 tgtgttaggg ctaacatatt taagattaat tggtaatcta gactgtctct ctaatttcat
    20641 ctgaagtaaa gttttcatgc aaaaatcttt attgaaaatg tcccagaact aaatatggta
    20701 tgttatcaga taacaagata taaagttgta tatgttataa tacatactgg cattatatgg
    20761 gaatagacac tactctacaa tgtggacatt tatctactgg gtttgtttat catacaaatt
    20821 tacattccag ggtccaccag gccctccagg accccctggt tcttctggac atcctggatc
    20881 tcctgtaagt atcattcttt ttggtgcttt cttcttcatg aatatatgaa gcaagctaat
    20941 gcagttatgg atataaatca gccatttcag tatccataat cccaaccaat aaataattca
    21001 ctccccaaca attattagca attaaatctt tggtaaaatt aaaatacata ttacatgtgt
    21061 atacaacaga ctttggagtc cataaccact caatttcggt gaaataaaag aaaactaact
    21121 ttattgtgat cctgaaaacc tcttaaatca tcataaaata taataaaatt tctgcctaca
    21181 tccactaacc atgacttctt acaataatcc agatgtgatt tactcatgtg aagctcttgt
    21241 tacctaaata tatttacact attcttctat tttagggttc tcctggatac caaggtcccc
    21301 ctggtgaacc tggtcaagct ggtccggcag taagtaacac ttgagaattg ctaaacacat
    21361 tgaaaattca tttcccacat gaattctatc atcagtttct aagtcattca ttgttcatct
    21421 agagtgtaga ttgcacacat gcttgaattt tccatgacaa tatagctttt tttctgtgag
    21481 gaacttgagg tgaactcaaa gactttagag attcctgact ggttaaccac caaaaatcat
    21541 ccacttccca gaaataacaa aatttcctat ctaggaaata catgtctaat gtttctgtag
    21601 attcgtttta tatgaattct accacaatgc ctacaaatat acatacatct gcatagtgca
    21661 gagagaaaga caagaatatc caagaataat atgccatata ggtatcaaaa attaaaataa
    21721 atgttatttt aagacaatat tttgaaatat tgcaaaatgt ttttatattt ttgtatagtc
    21781 attatctcaa aatatagaaa tacaaatata aatatacata tttagtatga catagataca
    21841 tgatatttaa atatttcact aaaatttatt caacactaaa acttaaatat aatgaaggta
    21901 taatattaat tcagaataat tttcatggtg aggacagagg gatgtttcaa agacacattt
    21961 gcttccagaa tgcaaatatc atacaacaag aaaatgtaac acaaaatatt gaatatttct
    22021 gtttctattt tcttatttca gggccctcca ggacctcctg gtgctcttgg tccagctggt
    22081 cctgctggaa aggatgtaag tacttataag ttaaatagaa atacagcaac attcatgttg
    22141 aatgacagac tgccttaaat gtatccactt ttgaagaatc ttagtaatgg aagcctcttt
    22201 caaaaaaatt gtacccaaca aacattttag ctcatattag aggtt
//