LOCUS       210262#pL1L2GT1_LF2A_nEGFPOT2A_CreERT2_pAbActNeopA#L3L4_pD223_DTA_T_spec        20959 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2GT1_LF2A_nEGFPOT2A_CreERT2_pAbActNeopA
COMMENT     design_id: 210262
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000068584
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     misc_feature    4275..10665
                     /note="5 arm"
     misc_feature    10693..10717
                     /note="B1 Gateway"
     misc_feature    11684..11701
                     /note="FCHK QC critical region"
     misc_feature    11668..11683
                     /note="FCHK QC prefix region"
     misc_feature    11702..11707
                     /note="FCHK QC suffix region"
     misc_feature    11830..11881
                     /note="F2A"
     misc_feature    11707..11829
                     /note="1Dleader"
     primer          11553..11574
                     /vntifkey="27"
                     /dnas_title="seq primer FCHK2"
                     /label=seq primer FCHK2
                     /note="seq primer FCHK2"
     exon            11653..11706
                     /vntifkey="61"
                     /dnas_title="mouse En2 exon"
                     /label=mouse En2 exon
                     /note="mouse En2 exon"
     misc_feature    12753..12753
                     /note=" A to G to kill RsRII site"
     misc_feature    12703..12756
                     /note="self-cleaving' 2A peptide"
     misc_feature    12757..13782
                     /note="Cre"
     misc_feature    11887..11976
                     /note="3X NLS"
     misc_feature    11977..12693
                     /note="EGFP"
     primer          complement(10846..10867)
                     /vntifkey="27"
                     /dnas_title="LRPCR LAR3_EN2"
                     /label=LRPCR LAR3_EN2
                     /note="LRPCR LAR3_EN2"
     intron          10778..11652
                     /vntifkey="21"
                     /dnas_title="mouse En2 intron"
                     /label=mouse En2 intron
                     /note="mouse En2 intron"
     primer          complement(10742..10765)
                     /vntifkey="27"
                     /dnas_title="FRT3_oligo"
                     /label=FRT3_oligo
                     /note="FRT3"
     misc_feature    10730..10777
                     /vntifkey="21"
                     /dnas_title="Frt"
                     /label=Frt
                     /note="Frt"
     misc_feature    15043..15050
                     /vntifkey="21"
                     /dnas_title="polylinker"
                     /label=polylinker
     misc_recomb     15011..15042
                     /note="Rox(inv)"
     misc_feature    15003..15010
                     /vntifkey="21"
                     /dnas_title="RE sites"
                     /label=RE sites
     primer          14943..14961
                     /vntifkey="27"
                     /dnas_title="seq primer NF"
                     /label=seq primer NF
     primer          14817..14840
                     /vntifkey="27"
                     /dnas_title="LRPCR RAF5"
                     /label=LRPCR RAF5
     misc_feature    14760..15002
                     /vntifkey="21"
                     /dnas_title="SV40 pA"
                     /label=SV40 pA
     misc_feature    13792..14736
                     /note="ERT2"
     misc_feature    16250..16250
                     /note="G to C conservative mutation to kill RsrII site"
     misc_feature    16598..16841
                     /note="SV40pA"
     misc_feature    16656..16679
                     /note="RAF5"
     misc_feature    16405..16424
                     /note="neoBstB1_5"
     misc_feature    16112..16130
                     /note="neoSphI_5"
     misc_feature    15775..15794
                     /note="neoPstI_5"
     misc_feature    15606..16591
                     /note="neomycin resistance "
     misc_feature    15456..15475
                     /note="betact5"
     promoter        15060..15580
                     /note="human beta actin promoter"
     insert          14757..16836
                     /source="pL1L2_GTIRES_BetactP FLFL MapVerified"
     misc_feature    16837..16841
                     /note="for bActNeo"
     misc_recomb     16843..16874
                     /note="Rox"
     misc_feature    16887..16907
                     /note="B2 Gateway"
     misc_feature    10666..16937
                     /note="Synthetic Cassette"
     misc_feature    16938..20959
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggaatagtg aaaccaccaa actagactac tccaagggaa taaaaaggtt
     4321 attttggaac aaaactgtct cagagagtag agggcaatga cttggcagga aagcaagcag
     4381 attttatatg acagggtagg agtcttgaat gcagagatca gcatgcctgt ctcctgggat
     4441 taaaggtgtg taccaccatg cctggatcta aatttagctg ggcaggatct tgcccgaagg
     4501 tcccacttcc ttaatctgtt atctcctaga acacaggatt ttgctccatt tcacttcctg
     4561 gtaccccttt aatactcaaa ccacatatta tatatttttt ttcctttcta agcttgctat
     4621 gtttgttcta acttcttttc ataagactta accagagaac aaagtctctg ctgggctttt
     4681 ttgaaacttc ctttgtcaaa gcaattaatc tgaatagcct caccttagcc tcaggcagac
     4741 ttttcagaca agggcaaaag tagccacatt cttcgacaaa ataccacaaa aatagtcttt
     4801 atgccacatt ctgagattct tctcctttga aatctcttgg gccaggtcaa caccgttcaa
     4861 attactccca gtaacagtct tccatattcc tactaggatg acccattaag ccccatttaa
     4921 agcattccac tgtgttccaa atccaaagtc cccaaatcta cattctttca attaaaagca
     4981 tggtcaggcc tattgcagca ataccccact cccagtacca acttctgtct tagttagagt
     5041 tttactgctg taaacagaca ccatgactaa ggcaagtctt attaaaaaaa aaaaaccaac
     5101 atttaaatgg gacgggctta caggttcaga ggttcagtcc attatcatca aggtgggagc
     5161 atggcagtat ccaggcaggc atggcgcagg cagagctgag agttctacgt cttcatccaa
     5221 aggctatagt gcaagactgg cttacaggta gctagggtga gagtattaag cccacaccca
     5281 catgacacac ctactccaac caggtcacac ctattccaat aaggccacac ctccaaatga
     5341 tgtcactccc tggcccaaga atatacaaac tatcacaagt ctctagcaga cataggttgt
     5401 ttgagctaat tgggaacaag gtatactttg cttgcattag gctcagagaa tggcaatagt
     5461 ggctttccct gacaaatagg gacccaaatc acaaggtgtc tgaggcctgg ctgggtttag
     5521 gtttcagttt acctggtaag agttctgttc acctggcttg ggcctgcggc catcatctta
     5581 ggacatcatc agtagcattg ccttttgtgg gccctgcttt gtgacataac agataagttc
     5641 tcaacaaatg gtggagtgga tagatggact ggaaggatga tggaatactg aaatggaggc
     5701 ttgaatgaat tttgaaagtt ctggagccat agatcctttt atgtttggga tctgtcttct
     5761 gatatcttct tcaatttctt tagtgacttg aagtttttat catccaagta tttcgcttgc
     5821 ttgattagca ttaacacaag ataccttata ttatttgagg cttttgtgaa gggtgtagtt
     5881 tcccttacct gtttctctgt ctgtttgcca ttggtacata ggagagctac tgacttaatt
     5941 ttgtatccag ctactttgct tgaaactgtt tatcagatat tggagttccc agtggaattt
     6001 ttaggatcac ttatatatac tgttatatca tctgcaaata aagaaacttt gacttcttct
     6061 gttctaattt gtattccctt gatctctgtc agttgcctta ttgctctagc taagacttca
     6121 agtactatat tgaataggta tggagagagc agacaacctt gttttgtttc gaattttagt
     6181 ggaaatgctt tgaatttatc tacatttaat ttgatgttgg ctactggctt gctataaact
     6241 cactttactt atgtttaggt acgtttcttg tagctctagt ctctccagga cttttatcag
     6301 gaaggacttt ggattttgtc aaagggcttt ctgcatctaa agagatgatc atgtggtttt
     6361 ttttaaattt gagtttattt atatggtggg atatatctat atctatctat ctatctatct
     6421 atctatctat ctatctatct atctatctaa tctctatcta tctatctatc tatctatcta
     6481 tctatctatc tatctatgat atattgatat atgtatgttg aaccatccct gtatctctgg
     6541 gcttaagcct actttatcat ggtggatgat atcttttgat gtgtcttgga ttcagtttgt
     6601 aagtatttta ttgaattttt tacatgaata ttcataaggg aaactgacct gtaattatct
     6661 ttctttgttg agtctttcta tggtttgagt atcagggtat ttgtggcctc acaaatatga
     6721 attggacaat ggtccttctg cttctatttt tttttttgga ataatttaag gagtattagc
     6781 attaactctt ctttgaaaat ctggtaaaat tctgagccat gactggccat gatcattttt
     6841 ttggttggaa gattttttaa tccctgagac tgcttctatt tcactaggtt tgtttaattt
     6901 tcttatcttg atttaacttt gttaaaggta atgtataaaa aaatatccat ttcttttaga
     6961 ttttccaatt tggtggagtg taggtcttta aagtattttc ttgtgatttg tctccttttt
     7021 gtttctgatt ttgttcattt gggcattcac ccttagcttt ttagttaatt tggataaaaa
     7081 atatgtcaaa tttctcaaag taccaactct ttgtttcatt gatttgttta ttttcctttc
     7141 tttctatttc attgatttca gccctgagtt tctttcttgc cttctactcc tcttgggtgt
     7201 gattactttt ttttttcttt tctaaagctt tcaggtgttc tgttaagtta ctagaatgag
     7261 attgctccac attttttttc atttttctta tagacattta ctgctataaa cttgactctt
     7321 tgcactgctt taactgtgtt ccagaagttt gggtacgctg tgtactcatt ttcactcaat
     7381 tctagaaagg ttttaattta tttcttaatt ctctcttggt ctattttcca tctagtagag
     7441 agttgttcag tttccatgaa tttgtcactt tttgttgttg atatctatct ttaatccatg
     7501 gtggtaagag aggatgcacg gagttatttc aatttcttta atatcttttg tatcttttat
     7561 tgctttgtgt ctgaaagtcc catgaggtgc agagaagaag gtgcattctt tttctgtcta
     7621 gttgaaatgt tctgtagaca tgtattggct tcatttagtt tttaatgtct cttaactcca
     7681 gtattttttt gttgttaagt ttttgtctgg atgacctgtc cattgtgaga gtttggtatg
     7741 gaagcctccc actattggtg tgtgaaggtt aaactgtagt agtgttgctt ttacaaatgt
     7801 gggtgtactt gtgtttgtac aagataacaa aatggctaca ccagcttgct tcttaggtct
     7861 atttccttgg aatatatttt cccatgcttt tacactgagg tgatgtctat ccctgatgtt
     7921 gaggtgtgtt tcttagctgc agcaggatgg atcctgtttt gcctccattc tgttattctg
     7981 tgtcttttat tgggggaatt gagaccactg ataatgagag atatcaatga atagcacttg
     8041 ttgatgccta ttattttgtt gttgttgata ctgtgttttt tccttctttt gactttgcat
     8101 gtctgggatt acttattccc tatattttct tggatgtagt tatcctcttt aggttgatgt
     8161 tttctttcta gcatcttctg aaaggttgga gtcttagaca gatactgatt aactttggat
     8221 tttctcgtga aatatcttat tttctccagg tattgtaatt gaaagtgttt atgagtataa
     8281 tagtcagggt ctcttggagt cttgcagcac actgtccaga tacttctggc ttttagagtc
     8341 tccactgagt agtcagtata agtctaatat gtctgctttt ataaattacg tattctttag
     8401 ctcctttgaa gcttctaaca ttgctttaac aattttaaca atttttgttt gttctgaaca
     8461 tttagtgttt tgattattat gtgccaagga agtctttctt ttgtggtcga gtctatttgg
     8521 agttctgtga gcttctaata ctttgctagg catctccttc tttagattat ggaaactttc
     8581 ctctattatt tcattgaaaa tatattctat gcctttgacc tatgtttctt ctcctacctc
     8641 tatttctatt attcatagat atgctctttt cgaagtattc caggtttctt gaatgttttg
     8701 tgtcaggagt tttttagttt taatactttc tttgaatgat gtatccactt aatctattgt
     8761 gtctttgatt gcgatcctct cttccatctc ttatattctg tttgctgaag cttgccactt
     8821 tgcttcctgt tcgtgttcct aaatttttca ttttctgatt tcctacagat tggtttgatt
     8881 ctttttccac tttcaggtct tgaagggttt gattcatttc tttccactgt ttgttggtgt
     8941 ttacatagac ttcttcaaga gacatattca ttttgtcttt actctgtcac attcataaat
     9001 gctgttttga tctttttctt gtgcctcaga taggttgcaa tattcagtat tcaatattct
     9061 gggctctagt ggagtcatgt tgtcttggct attattggtt gtggttttac agtggcatct
     9121 aggcatctga gatttggaag actgtagctc ttggtgctgt tatctggtct tgtctttgtt
     9181 gggtgggtgt cttgttcctc ggtttttttt tttcacctct ttattttagg agggtgtggt
     9241 atctgtgtgt tgcctgggtg gtgtggtcac tggagatttc aagtaaaatg gaatcttttt
     9301 tatgggtatt gggagctgac acttatacct ttggagaggg tttggactgg caagactgag
     9361 tggggtccat agactgagga aagcaaggtg ttccacaagg atttacttag tcccctggaa
     9421 atgggtacgg actgggggaa ggccacagca gaaggtctgc tacagagctg gggatgagac
     9481 tgggaggttg gacttggagg aggaatggag agagaataaa gatctatagt cagtctactt
     9541 gcttccctgg tcagagtggc cttgggttct caggaagtgc ctgctggagt tggagactgg
     9601 gacaaagcaa tgggttgtgg taaggaagtc tggatgggaa tatctgtgcg atccatgggt
     9661 gatagggggg aaagtgttct gctgcagatc tggagatgaa cctgggggat ttgaactgta
     9721 ggagcagaag gagacttaaa ggtcttcagt cagctagctg cttccctggc aggaatattt
     9781 acttcttagc tcaagggcct ctgatgatta acactctgac atattcccac cttctcatat
     9841 agtgactgac acatgattct caacaagtat taggtcttaa acttcttcta attcacatta
     9901 gtggctatgg ctccaaagag atttccctag ctttcctcca ttctctattc tatactccaa
     9961 agagtggctg agtccaaacc taccctgtcc ctttctttac tatagtaaca agatattata
    10021 cagttaatga ttttttgaga caggggcctc aagcttacta tgttgacaaa gatgaccttg
    10081 aacttgtgat acttgagata gtgcctatat ctcctgaagg ctagtattat aggcaaacac
    10141 tctatcaact aagcagcaca ccgccaccac cccagtccat gtttatctgt ttttatgctc
    10201 aggtgcacac ttgtgtatta aatatgttag attataatta tataataacg atttcagtga
    10261 ctaatgtgct aattgaatac aactgaacaa ataggaaaca ggcccataga gggatactta
    10321 ggactgattt atttcaaatt ctgtgagaga gagacagaga gagagagaga gagagagaga
    10381 gacagagaga cagagagaca gagagacaga gagacagaga gacagagaga cagagacaga
    10441 gacagagaca cagacacaca gagagacaca gagagacaga gaggggggaa aggagagagc
    10501 ctgagaggct atgaatgaga tagtatcagg atcatcttag tgtccttcgt cttcttgtat
    10561 aacagcagaa atttgggatt ataattggta agttctgttc caccctgtcc tgccttaccc
    10621 catttcatag gctctaactt cttcctcttc taccagctca agttaaaggc gcataacgat
    10681 accacgatat caacaagttt gtacaaaaaa gcaggctggc gccggaaccg aagttcctat
    10741 tccgaagttc ctattctcta gaaagtatag gaacttcgaa ccctttccca caccaccctc
    10801 cacacttgcc ccaaacactg ccaactatgt aggaggaagg ggttgggact aacagaagaa
    10861 cccgttgtgg ggaagctgtt gggagggtca ctttatgttc ttgcccaagg tcagttgggt
    10921 ggcctgcttc tgatgaggtg gtcccaaggt ctggggtaga aggtgagagg gacaggccac
    10981 caaggtcagc cccccccccc tatcccatag gagccaggtc cctctcctgg acaggaagac
    11041 tgaaggggag atgccagaga ctcagtgaag cctggggtac cctattggag tccttcaagg
    11101 aaacaaactt ggcctcacca ggcctcagcc ttggctcctc ctgggaactc tactgccctt
    11161 gggatcccct tgtagttgtg ggttacatag gaagggggac gggattcccc ttgactggct
    11221 agcctactct tttcttcagt cttctccatc tcctctcacc tgtctctcga ccctttccct
    11281 aggatagact tggaaaaaga taaggggaga aaacaaatgc aaacgaggcc agaaagattt
    11341 tggctgggca ttccttccgc tagcttttat tgggatcccc tagtttgtga taggcctttt
    11401 agctacatct gccaatccat ctcattttca cacacacaca caccactttc cttctggtca
    11461 gtgggcacat gtccagcctc aagtttatat caccaccccc aatgcccaac acttgtatgg
    11521 ccttgggcgg gtcatccccc cccccacccc cagtatctgc aacctcaagc tagcttgggt
    11581 gcgttggttg tggataagta gctagactcc agcaaccagt aacctctgcc ctttctcctc
    11641 catgacaacc aggctcccag gtcccgaaaa ccaaagaaga agaacgcaga tcgcagatcg
    11701 cagatctcta gagtcaccga gttgctttac cggatgaaga gggccgaaac atactgtcca
    11761 aggcccttgc tggcaatcca cccaactgaa gccagacaca aacagaaaat tgtggcaccg
    11821 gtgaaacaga ctttgaattt tgaccttctc aagttggcgg gagacgtcga gtccaacccc
    11881 ggaccggtgc atgtggaccc caagaaaaag cggaaggtgg accctaaaaa gaaacgcaaa
    11941 gtggatccta agaaaaaaag aaaggtgcac gtgtccatgg tgtccaaggg cgaggaactg
    12001 ttcaccggcg tggtgcccat cctggtggaa ctggatggcg acgtgaacgg ccacaagttc
    12061 agcgtgtccg gcgagggcga aggcgacgcc acatacggaa agctgaccct gaagttcatc
    12121 tgcaccaccg gcaagctgcc cgtgccttgg cctaccctcg tgaccacact gacctacggc
    12181 gtgcagtgct tcagcagata ccccgaccat atgaagcagc acgacttctt caagagcgcc
    12241 atgcccgagg gctacgtgca ggaaagaacc atcttcttta aggacgacgg caactacaag
    12301 accagggccg aagtgaagtt cgagggcgac accctcgtga acagaatcga gctgaagggc
    12361 atcgacttca aagaggacgg caacatcctg ggccacaagc tggagtacaa ctacaacagc
    12421 cacaacgtgt acatcatggc cgacaagcag aaaaacggca tcaaagtgaa cttcaagatc
    12481 cggcacaaca tcgaggacgg ctccgtgcag ctggccgacc actaccagca gaacacccct
    12541 atcggcgacg gccctgtgct gctgcctgac aaccactacc tgagcaccca gagcgccctg
    12601 agcaaggacc ccaacgagaa gagggaccac atggtgctgc tggaattcgt gaccgccgct
    12661 ggcatcaccc tgggcatgga cgagctgtac aagcctgcag gcgagggcag aggaagtctt
    12721 ctaacatgcg gtgacgtgga ggagaatccc gggccgtcca atttactgac cgtacaccaa
    12781 aatttgcctg cattaccggt cgatgcaacg agtgatgagg ttcgcaagaa cctgatggac
    12841 atgttcaggg atcgccaggc gttttctgag catacctgga aaatgcttct gtccgtttgc
    12901 cggtcgtggg cggcatggtg caagttgaat aaccggaaat ggtttcccgc agaacctgaa
    12961 gatgttcgcg attatcttct atatcttcag gcgcgcggtc tggcagtaaa aactatccag
    13021 caacatttgg gccagctaaa catgcttcat cgtcggtctg ggctgccacg accaagtgac
    13081 agcaatgctg tttcactggt tatgcggcgg atccgaaaag aaaacgttga tgccggtgaa
    13141 cgtgcaaaac aggctctagc gttcgaacgc actgatttcg accaggttcg ttcactcatg
    13201 gaaaatagcg accgctgcca ggatataagg aatctggcat ttctggggat tgcttataac
    13261 accctgttac gtatagccga aattgccagg atcagggtta aagatatctc acgtactgac
    13321 ggtgggagaa tgttaatcca tattggcaga acgaaaacgc tggttagcac cgcaggtgta
    13381 gagaaggcac ttagcctggg ggtaactaaa ctggtcgagc gatggatttc cgtctctggt
    13441 gtagctgatg atccgaataa ctacctgttt tgccgggtca gaaaaaatgg tgttgccgcg
    13501 ccatctgcca ccagccagct atcaactcgc gccctggaag ggatttttga agcaactcat
    13561 cgattgattt acggcgctaa ggatgactct ggtcagagat acctggcctg gtctggacac
    13621 agtgcccgtg tcggagccgc gcgagatatg gcccgcgctg gagtttcaat accggagatc
    13681 atgcaagctg gtggctggac caatgtaaat attgtcatga actatatccg taacctggat
    13741 agtgaaacag gggcaatggt gcgcctgctg gaagatggcg atctcgagcc atctgctgga
    13801 gacatgagag ctgccaacct ttggccaagc ccgctcatga tcaaacgctc taagaagaac
    13861 agcctggcct tgtccctgac ggccgaccag atggtcagtg ccttgttgga tgctgagccc
    13921 cccatactct attccgagta tgatcctacc agacccttca gtgaagcttc gatgatgggc
    13981 ttactgacca acctggcaga cagggagctg gttcacatga tcaactgggc gaagagggtg
    14041 ccaggctttg tggatttgac cctccatgat caggtccacc ttctagaatg tgcctggcta
    14101 gagatcctga tgattggtct cgtctggcgc tccatggagc acccagtgaa gctactgttt
    14161 gctcctaact tgctcttgga caggaaccag ggaaaatgtg tagagggcat ggtggagatc
    14221 ttcgacatgc tgctggctac atcatctcgg ttccgcatga tgaatctgca gggagaggag
    14281 tttgtgtgcc tcaaatctat tattttgctt aattctggag tgtacacatt tctgtccagc
    14341 accctgaagt ctctggaaga gaaggaccat atccaccgag tcctggacaa gatcacagac
    14401 actttgatcc acctgatggc caaggcaggc ctgaccctgc agcagcagca ccagcggctg
    14461 gcccagctcc tcctcatcct ctcccacatc aggcacatga gtaacaaagg catggagcat
    14521 ctgtacagca tgaagtgcaa gaacgtggtg cccctctatg acctgctgct ggaggcggcg
    14581 gacgcccacc gcctacatgc gcccactagc cgtggagggg catccgtgga ggagacggac
    14641 caaagccact tggccactgc gggctctact tcatcgcatt ccttgcaaaa gtattacatc
    14701 acgggggagg cagagggttt ccctgccaca gcttgatgaa gatctgagct cttaattaat
    14761 gatcataatc agccatatca catctgtaga ggttttactt gctttaaaaa acctcccaca
    14821 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
    14881 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
    14941 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
    15001 ccgtttaaac taactttaaa taattggcat tatttaaagt tacatatggc ggccaattcg
    15061 ccttctgcag gagcgtacag aacccagggc cctggcaccc gtgcagaccc tggcccaccc
    15121 cacctgggcg ctcagtgccc aagagatgtc cacacctagg atgtcccgcg gtgggtgggg
    15181 ggcccgagag acgggcaggc cgggggcagg cctggccatg cggggccgaa ccgggcactg
    15241 cccagcgtgg gcgcgggggc cacggcgcgc gcccccagcc cccgggccca gcaccccaag
    15301 gcggccaacg ccaaaactct ccctcctcct cttcctcaat ctcgctctcg ctcttttttt
    15361 ttttcgcaaa aggaggggag agggggtaaa aaaatgctgc actgtgcggc gaagccggtg
    15421 agtgagcggc gcggggccaa tcagcgtgcg ccgttccgaa agttgccttt tatggctcga
    15481 gcggccgcgg cggcgcccta taaaacccag cggcgcgacg cgccaccacc gccgagaccg
    15541 cgtccgcccc gcgagcacag agcctcgcct ttgccgatcc tctagagtcg agatccgccg
    15601 ccaccatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
    15661 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
    15721 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
    15781 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
    15841 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
    15901 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
    15961 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
    16021 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
    16081 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc
    16141 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
    16201 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcc gaccgctatc
    16261 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
    16321 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
    16381 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
    16441 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
    16501 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
    16561 cttcgcccac cccccggatc taagctctag ataagtaatg atcataatca gccatatcac
    16621 atctgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
    16681 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
    16741 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
    16801 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cctaacttta aataatgcca
    16861 attatttaaa gttaggaatt aattcaccca gctttcttgt acaaagtggt tgatatctct
    16921 atagtcgcag taggcggggt ttctttcgaa ggaagaaagg tgtgaggttc ctactccacc
    16981 ttctgacaga gagcgttgtg tcgagtctca aaggtgtcac tgacattcga gtgacttggc
    17041 ttcttctgtc tgactccaca gccacctacc agcagccagc tcactttatc ttttccaaga
    17101 gttgagtcct gaataattat aggacagtct gagatatcag aaccgctagg aaagtagaaa
    17161 aacggtagag tagttcaggc aagaaagtaa ggaaagtgga tgagtgagag agagcaagag
    17221 tctcatccac aggtagagac tgtgccagta tgatgctcct tggtaaaggt aggaaagaaa
    17281 ctctaggctt catgattcag acttctctgt gttgcctaga agcatctgtt cacatagcgt
    17341 cactcccctt taattcagac ctacacgggt accattgaag tcctagattt tagagtcagt
    17401 cacatctggg accactcctg atatagtcac tgtattagtt atttttattg tcccacacta
    17461 tagtgataag aatgtgcttt tgttttaatc ccaggtgtgg ggacgtgggg ctcctttgga
    17521 ctgttcacag cagctgacta tgatttgcct catgccctag cagaggtgtg gttttgccac
    17581 ttacagatag tttctgcaac tgtgtaatgt ttagaattct aggtaatttt ttagaaggac
    17641 tttcatatag atgctaggtc ccagggagtc aaggtttgtg gttggtggta gtttagaaaa
    17701 gttgtttttg gtttgttagt agttgtgctc aaagaagaaa caaaagggaa aaaaaattag
    17761 attgttagat tcagggatct ctctctctct ctctctctct ctctctctct ctctctctct
    17821 ctctctctct ctctctctct ctctctctct ctctctctct ctctccccta tcatttttct
    17881 ctcttctatc tagtgttagg gggtaaaatt ggcaggaggt agataaggcg gtgaaaagaa
    17941 gaacccacaa agtagctaag accagctaca ccataccaaa acagcataag ataggacatg
    18001 taagaataca gtccatcacg acaggcaagg cgtggcaatg gaagtgtcag atggcttttc
    18061 acaccatgtc cacagtcagg aagcagagaa atgaatgttg gtgttcaaag gcttccttct
    18121 tttcaccttc attaagttgg aggcctcagc ttatggaatg gcattgccca cattgagtag
    18181 agaccttccc tactcattta aatagctctg gaaacatcct taacagatgc tcacagatgt
    18241 gtctcttagg tgattccata gcccaccaaa ctgacaaccg agtttaatta taacagcctg
    18301 agtttaactg tcatatctat ggctaactaa gatgtttaat ttctccaagc tatgctttct
    18361 taaatgtaag gcaaaataat tgttcttaca atgctataag gaataaatga atgatgcacc
    18421 taatccatat aacacagatc ctggcaaatt aatcacaaat ggtaaatagc aatggtaagg
    18481 taatagcatc taagttagtt ttggtttctc taactctgga aacaactgga cttgggggac
    18541 catttaatga aattgtctac aacttccaga actctagcta ccccatggtc catatttaaa
    18601 agtgctatcc ttttgcccca atgtacacag ttttccagaa tagttcaaac ctacagtagc
    18661 aatttttttc tgtgaccatg ttatggtcag ataactctcc tggcaggcat aaagatattc
    18721 ttaagaggca aatatactct ccatttgggg atagataatt tcaatattag ctgttgaaag
    18781 ggaaacacgt tggctcatct aatcccagca cttgggagac taaggcagga ggataaaata
    18841 ctttggagcc agtatagtct tcctagtgag accctgtctc aaagttaaca actaaaatgg
    18901 gagtaacttc cactaatcct tccaaaagta actaaagtca agggcttatg gagaaattat
    18961 tcttatgccc aattacctga cactcactgt cttctcaccg tggctcctat ttatattata
    19021 tttgtgatat ttgtttcaga tgcaagaatg tttcacctca agactgatgt ctcaagtagc
    19081 tatgcagaag aggaggcaga aaggttgtaa gaggcagtag ggatgggaga caccaaggaa
    19141 gcaatgcctt ccagacataa caggattgat gcacatatga actcatagag accatgtgtc
    19201 ttagtttggg ttttactgct gtgaagagac actatgacca aggcaactct tataaaggac
    19261 aatatttaac taaggctggc ttacaggttc agaggttcaa tccatttaca tcaaggaagt
    19321 agcatggcag catccaagca ggcatggtac aggaagagct aagagttcta catcttgttc
    19381 tgaaggcaaa caggagcaga ctggctaact gacagctagg agtagggtct taaagctcac
    19441 acccacagtg acacacatac tccaacaagg tcacatctcc taatagtgcc attccttagg
    19501 ccaagcatat acaaaccacc acaccatgac agcatgtgca gggccaggac aggtctaagc
    19561 cagatgggat cccagcactg atagggggaa gtggacatga gcctccatcc ctaacccaaa
    19621 agctatttcc aagtgtcaat ctcttttttt taaagaattt atttatttgt ttaatgtata
    19681 tgagtacacc attgccctct tcagacacac cagaagaggg catcagaccc cattgcagat
    19741 ggttgtgagc caccatgtgg tttctgggaa ttgaactcaa gacctccaga agagcatcag
    19801 tgctcttaac cactgagcct aagtgcaatg gaaaatttag ttttgtccag tgaagcctta
    19861 ctgggtatac aaaccatact gaaggacagg cccatgccta gcagtaggtg gccaacacaa
    19921 aatgaactca gtggtattct ggaaagtttt gttgttttgt gtgtgtgtgt gtttgtgtgt
    19981 gtgtgtgtgt gtgtgtctca taatgatttg tttggacatt tataaaaaaa actttgtctg
    20041 gtcttttgtt aatatattat ggcttctgat tttatgtatc tgtatgtgta tgtgtttctc
    20101 attctttttc tttgtcttgt catttgtttg ttttattctg gtttgtttgt ttgcctgttt
    20161 tctaaagaaa tagagaatga agatgcaggg ttggatgggt ggggagatgg ggaagatctg
    20221 ggagaagata aaagagggaa catcatgatc agaatatatt atatgaaaca ccaatgacaa
    20281 caaaaacacc gaggtgtaaa aactaagaga agggcaatgt aacactcaag atactgtaac
    20341 cttcattttg gatatcagac cattaaaaca atgggcactg caatggtttg aatagagata
    20401 ccccccctca gtcccctgcc caggcacatg tatttgaatg cttggttagg gagtgttgct
    20461 tctgtacagg gattaggaag aacagccttg ttagaggaag tgtgtcactg ggggtggggg
    20521 tgggggtggg gagcttttgt agtttcaaat gctcaagcca tgtcaagtct ctctctcttc
    20581 ttgttgcctg gcagtccaga tatacagtcc ttctgcaaca ccatttctgc ctgcatgctg
    20641 acatgcttcc tgctatgcgg ataatgggct aaacctcaga aactataagc cagccccaag
    20701 ttaatgcttt tctttataag aatttctgtg aacatgatgt ctcttcacag caatagaaca
    20761 ctaagacaga ccccaagaaa ttggaatttc tccaggaatc tgatattggt agagtaaaag
    20821 gctagagaaa ggacttgttg ccagggaaga gatttcacat ttatcacaac ttgccagaac
    20881 accttgcggg agagattaaa accatgtggg accatgcttt gattaagact gcttacttat
    20941 gtgtacctct tgctttcta
//