LOCUS       239330#pL1L2GT0_LF2A_nEGFPOT2A_CreERT2_pAbActNeopA#L3L4_pD223_DTA_T_spec        20428 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2GT0_LF2A_nEGFPOT2A_CreERT2_pAbActNeopA
COMMENT     design_id: 239330
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000042755
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            4729..4880
                     /db_xref="ENSEMBL:ENSMUSE00000390049"
                     /rank="1"
                     /note="ENSMUSE00000390049"
     exon            5713..5764
                     /db_xref="ENSEMBL:ENSMUSE00000325553"
                     /rank="2"
                     /note="ENSMUSE00000325553"
     exon            6665..6785
                     /db_xref="ENSEMBL:ENSMUSE00000325547"
                     /rank="3"
                     /note="ENSMUSE00000325547"
     misc_feature    4275..9496
                     /note="5 arm"
     misc_feature    9524..9548
                     /note="B1 Gateway"
     misc_feature    10515..10518
                     /note="FCHK QC critical region"
     misc_feature    10499..10514
                     /note="FCHK QC prefix region"
     misc_feature    10519..10524
                     /note="FCHK QC suffix region"
     misc_feature    10647..10698
                     /note="F2A"
     misc_feature    10524..10646
                     /note="1Dleader"
     primer          10384..10405
                     /vntifkey="27"
                     /dnas_title="seq primer FCHK2"
                     /label=seq primer FCHK2
                     /note="seq primer FCHK2"
     exon            10484..10523
                     /vntifkey="61"
                     /dnas_title="mouse En2 exon"
                     /label=mouse En2 exon
                     /note="mouse En2 exon"
     misc_feature    11570..11570
                     /note=" A to G to kill RsRII site"
     misc_feature    11520..11573
                     /note="self-cleaving' 2A peptide"
     misc_feature    11574..12599
                     /note="Cre"
     misc_feature    10704..10793
                     /note="3X NLS"
     misc_feature    10794..11510
                     /note="EGFP"
     primer          complement(9677..9698)
                     /vntifkey="27"
                     /dnas_title="LRPCR LAR3_EN2"
                     /label=LRPCR LAR3_EN2
                     /note="LRPCR LAR3_EN2"
     intron          9609..10483
                     /vntifkey="21"
                     /dnas_title="mouse En2 intron"
                     /label=mouse En2 intron
                     /note="mouse En2 intron"
     primer          complement(9573..9596)
                     /vntifkey="27"
                     /dnas_title="FRT3_oligo"
                     /label=FRT3_oligo
                     /note="FRT3"
     misc_feature    9561..9608
                     /vntifkey="21"
                     /dnas_title="Frt"
                     /label=Frt
                     /note="Frt"
     misc_feature    13860..13867
                     /vntifkey="21"
                     /dnas_title="polylinker"
                     /label=polylinker
     misc_recomb     13828..13859
                     /note="Rox(inv)"
     misc_feature    13820..13827
                     /vntifkey="21"
                     /dnas_title="RE sites"
                     /label=RE sites
     primer          13760..13778
                     /vntifkey="27"
                     /dnas_title="seq primer NF"
                     /label=seq primer NF
     primer          13634..13657
                     /vntifkey="27"
                     /dnas_title="LRPCR RAF5"
                     /label=LRPCR RAF5
     misc_feature    13577..13819
                     /vntifkey="21"
                     /dnas_title="SV40 pA"
                     /label=SV40 pA
     misc_feature    12609..13553
                     /note="ERT2"
     misc_feature    14655..15237
                     /note="synthetic fragment mutates RsRII"
     misc_feature    15067..15067
                     /note="G to C conservative mutation to kill RsrII site"
     misc_feature    15415..15658
                     /note="SV40pA"
     misc_feature    15473..15496
                     /note="RAF5"
     misc_feature    15222..15241
                     /note="neoBstB1_5"
     misc_feature    14929..14947
                     /note="neoSphI_5"
     misc_feature    14592..14611
                     /note="neoPstI_5"
     misc_feature    14423..15408
                     /note="neomycin resistance "
     misc_feature    14273..14292
                     /note="betact5"
     promoter        13877..14397
                     /note="human beta actin promoter"
     insert          13574..15653
                     /source="pL1L2_GTIRES_BetactP FLFL MapVerified"
     misc_feature    15654..15658
                     /note="for bActNeo"
     misc_recomb     15660..15691
                     /note="Rox"
     misc_feature    15704..15724
                     /note="B2 Gateway"
     misc_feature    9497..15754
                     /note="Synthetic Cassette"
     exon            16152..16363
                     /db_xref="ENSEMBL:ENSMUSE00000325541"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 1 ENSMUSE00000325541"
     misc_feature    15755..16582
                     /note="Critical Region"
     misc_feature    16606..16639
                     /note="Downstream LoxP"
     misc_feature    16583..16662
                     /note="synthetic loxP region"
     exon            17408..17540
                     /db_xref="ENSEMBL:ENSMUSE00000325531"
                     /rank="5"
                     /note="ENSMUSE00000325531"
     exon            18476..18573
                     /db_xref="ENSEMBL:ENSMUSE00000325522"
                     /rank="6"
                     /note="ENSMUSE00000325522"
     exon            19811..19940
                     /db_xref="ENSEMBL:ENSMUSE00000325515"
                     /rank="7"
                     /note="ENSMUSE00000325515"
     misc_feature    16663..20428
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggaaaatat acttgaccga tggttttgag cgaaataata actggataat
     4321 caagaaatat atccactaat gaatagcctg aactactgaa acaatttgtt cagtgcctag
     4381 catatggtgt gcattttatt atttctttca aaaagaatgt atttggagtt acatagtaag
     4441 tctgctacct tttctttatg gctatatcta tgtcttatgt tgagatgaat gaattattct
     4501 tcaggggaaa taatctattt gaacagttta gatggtgaag aacatttgca gcatttgcaa
     4561 gatttttttc cactctgaag tggtctttgt ccttgaacat aggatacaag tgacccctgc
     4621 tctgttaatt attggcaaat tgcctaactt caacgtaagg aaatagagtc atatgtttgc
     4681 tcactgaagg ttactagtta acaggcatcc cttaaacagg atataaaagg acttcagcag
     4741 gactgctcga aacatcccac ttccagcact gcctgcggtg aaggaaccag cagccatgaa
     4801 gtggatcaca cccgcttccc tcatcctcct gctacatttc gctgcgtcca aagcattgca
     4861 cgaaaatgag tttgggatag gtaagatgct ttgtgacttg ggagctattt tcctataaca
     4921 aaattcttaa aaattcccat atttgcatct ccaggtatct tttttttttt tttttttttt
     4981 tttttttgag acagggtttc tctgtgtagc cctggctgtc ctggagctca ctttgtagac
     5041 caggctggcc tcgaactcag aaatccgcct gcctctgcct cccgagtgct gggattaaag
     5101 gtgtgcgcca ccacacccgg ctctccaggt atcttgactt aagcattcta tatactgttg
     5161 aagaccaggt atagatctaa gtggtagata gatttagatg taatgtaatt ttatgtaaga
     5221 ggcttataaa ataacagcag tgttcaacac aatcaatttt ggatgtgttc catgctcaat
     5281 ttttttttga tattaattat ttagtagggt ctcatgtcta gttccttcgg gttgacagaa
     5341 ataacatttt cactctcaat ctttttaaaa gtgctaattg ttcgactctc tggaaagcct
     5401 aatgtgaaca ggccgactgt agcataatta tgtccaaagc tactaaatga agccattttg
     5461 aatattatac tgtgagggag ccaagtagta aggggacctg aaagatgagt attagtacta
     5521 agtctgcaac tgatcattct ggttaacttt gttccttaat ttttcagtgt ccccaagtgt
     5581 aaaattaaga tgtctggaca ggtggttcct aacaacttgg cagttctaag acttcatggc
     5641 ttgggtttaa attccacaac gattgctttc ccggttaaaa tcctcacttg tccaagtctg
     5701 ttttcctttc agcttccacg ttagattcct cccagtgcgt gacggagaag aatgtgctta
     5761 gcatgtaagt ttcgcttcca tcaatgtgct gcagaaatgg cgtgcagaga taagtctagg
     5821 caaccgaatg tgaggtttga aattacagga gcaaattggg cagctttaag gctttgcttg
     5881 tgctgaatat cacactgata agcaacatgt ttaacttcgg tcaacgcttt agatcttagc
     5941 cttgacattg actggagttc cagaaagcaa gccaaatcag attttcaaaa ctgaagaatt
     6001 aatttctctg cagctttgta tgggctcagg agcgtgtctt gtcagaacca ataggaagat
     6061 attgcgatat cctctatttt gtaagctctt taatatcaag tcataggaag taaaatattc
     6121 ttagtttagt tattgtggag aatattttat tcctttaaat attgtctctg ttctacttct
     6181 ttgacattta tagtaaggaa tttaaacgtg tgtcactggt aatgtctgca tgtagaaagc
     6241 tggacacgga tcactttgag tcaatcggac actagtgcaa tctaggattg gggctcagtc
     6301 acgctgggag caaatataac tgaaggtcca gtgaccttca tctttgctta aattaagtaa
     6361 gaaaaatagt gtgaaaaatg tgggccttcc caggtataga tgctcagtga gcagtgaccc
     6421 caaaagagga agtatatttg ggggaaatct atttattttg acttttaaga cttgcacctg
     6481 tatttatggg tgaagatgac agaaagaagg ctagcatttc cctctgagtg actcgaaccc
     6541 agtttcccgt agatctttag caaagggctc cttctaaaca gatcagagca gaggaagctg
     6601 tacttgaaag gcacgcgtga ctgggtctca gcctcaagcc gctctgcatg tggtctcctt
     6661 gcagagctac catcaccttt acccagtttg ttccggaagc caccgaggag gaagtgaaca
     6721 aaatgactag cgatgtgttg gctgcaatga agaaaaactc tggcgatggg tgtttagaaa
     6781 gccaggtgag tgattagaga gaaagccaca ttgacactta accaaaattt aggctgcaga
     6841 aagatgtcag gaagttgtgg gtttaattat ctgaaaactg ttttctggaa gtagggttcg
     6901 attagttctg gaatcccgcc gaacagcact gttgaagcag agcaagcggc tgcttaaagg
     6961 agctgagatt aatacgagac tcagagctaa ggctccggag taacgctatc tgagcaacca
     7021 caaacacatt ctcttctttg agcttccata cctgcaatta tggagtgaca ttagatccac
     7081 tcagagctgt cacggaggtt tatgttgatg acgagtggca gtatattgcc tcttatgcag
     7141 acattgtggc tgacagcatt ctagggatca ctgcaaacac actgataatc aaaacgctgc
     7201 atggaaggtg acacccgcag aaaatctgtc cagtcaacta agagaaattg gctgcagcgt
     7261 atcatcagaa ttcctatctt atttataaat gttaatttat taaattataa tatttatatt
     7321 ttatacatat attacagttt acatactatg ttatattata tatggagaca tagtacatat
     7381 tatattgtat attatatatt acatattata ttatatatga acaatccata tttgtcatgt
     7441 agttataatt ttatatagtg ttatatcaca ttatacaata ataatttaat taattgacag
     7501 attaattaaa tctctatgat aatcctggaa agttgaggac aattttctca tggaggaaac
     7561 ttaggaacag ggaaatcact cctttggaga attatagttt ggtagacctg aggattaaat
     7621 gaagtcttct ggctccagaa ttaggtcctt agcaaggtgc tcacactgtt cagaacagca
     7681 cggtgagtgt gaggcctgca gcaggttatt cgagttgctg gatatagcac gtacgcagac
     7741 aggaacatca gtgtctgata tccaagtaag tcagaacttg agaaaaacaa gcacgtttac
     7801 acacccagtt gccctggtat agaaacaaaa tagctaccat tctgtagttt actcctgtat
     7861 ggaaatgtgc tttatacttg ttggagggat ttaagtatgg aatacataca gcagagcatg
     7921 ggcacagggc cttccgacgg tgcatatgcc aagggccgca ccctaaagaa agccactctt
     7981 taccttccag cagccagcaa ctgcccgtca ctcctcggca aggacccagg gtttgtgagc
     8041 ccttctccat ccacacaggc gttttgtgta caattgcctt gttatgtatg aaaagttttt
     8101 gcttcaatct tcctgcagtc tctggccctt aaaatccttt ctcccttctt ctccaatgga
     8161 ccctgattct tgaggggaga gagagagcct ttcctttctg tagacatccc atttggggct
     8221 gagcacatca tggctactta tcctctgtat tttggccagt tttgaacctc tgcatccatt
     8281 accatcccct gcaaatagag cctcctggga tgaagactgg ggctgcacaa agttctgcca
     8341 ctctgtgttt agaaggcact ctagtattat gcccattcag taaaacaaat agagataggt
     8401 tcactcttag ggcctatacc atactcagcc atgggttctt ggcccagctt gcagtgccag
     8461 gcctgagtgc caccctgtca gagagtactg tttgtcactc tcaggaatct atgtcactgt
     8521 tgtatcaatg gctatacctt agccaggctg actgtagctc ttagggttta cagctggggt
     8581 aaccccgtag atgacatcac atgacttttc tccccctagc ggtttgcaca tgcccttctg
     8641 gaattatgaa atctagccag taagaaggca acctcagacg cttctggctc tagaatttat
     8701 gggttaataa gaatctccgg tttgtatgtt agtttcagtc actctttgtt agtcagttaa
     8761 aaaggcgtgt ataaattatg cccaaagcac ttcttattgc gcctttcgta ttcagttagt
     8821 gttcagggta atgttgcaaa tggggtggct cgtcactaca agttcaaaag gtttctttta
     8881 aaaggttgac tgattctgta gccagaagat ggcctagtcg gccatcattg ggaagacagg
     8941 ccccttggtt ttgcaaactt tatagcctca gtacagggga ccgccagggc caagaagtgg
     9001 gagtgtgtgg gtaggggagt ggggggggga gggtatgggg aacttttggg atagcatttg
     9061 aaatgtaaat gaagaaaata cctaatttta aaaaagacaa taataaaaca aacaaacaaa
     9121 caaaacccaa ccaaccaacc aaataaaaaa aggttgcgtg aaacaaacat ggcggtgcac
     9181 cactatggtc tcagggcttg ggaggctgag gtaagtggat ctgagtttgg ggcgagtctt
     9241 gtttccatcg tgagtttcag gctagccgtg acttcatcag gaagaaaccc tatctcatcc
     9301 tcgtagagaa tatcacaaac caaaaatgaa tatagagaat gagtcttcag taagcctgtt
     9361 gtaatagtta aataaagcga ttcctaaggt gggtgacttc cttcaaaata ctggtcctgt
     9421 ccaaatctca gtgaatttag gtgctaagga agaaagcatt ttaaaatacc gtagctaaat
     9481 ctgtaattat gactaaaagg cgcataacga taccacgata tcaacaagtt tgtacaaaaa
     9541 agcaggctgg cgccggaacc gaagttccta ttccgaagtt cctattctct agaaagtata
     9601 ggaacttcga accctttccc acaccaccct ccacacttgc cccaaacact gccaactatg
     9661 taggaggaag gggttgggac taacagaaga acccgttgtg gggaagctgt tgggagggtc
     9721 actttatgtt cttgcccaag gtcagttggg tggcctgctt ctgatgaggt ggtcccaagg
     9781 tctggggtag aaggtgagag ggacaggcca ccaaggtcag cccccccccc ctatcccata
     9841 ggagccaggt ccctctcctg gacaggaaga ctgaagggga gatgccagag actcagtgaa
     9901 gcctggggta ccctattgga gtccttcaag gaaacaaact tggcctcacc aggcctcagc
     9961 cttggctcct cctgggaact ctactgccct tgggatcccc ttgtagttgt gggttacata
    10021 ggaaggggga cgggattccc cttgactggc tagcctactc ttttcttcag tcttctccat
    10081 ctcctctcac ctgtctctcg accctttccc taggatagac ttggaaaaag ataaggggag
    10141 aaaacaaatg caaacgaggc cagaaagatt ttggctgggc attccttccg ctagctttta
    10201 ttgggatccc ctagtttgtg ataggccttt tagctacatc tgccaatcca tctcattttc
    10261 acacacacac acaccacttt ccttctggtc agtgggcaca tgtccagcct caagtttata
    10321 tcaccacccc caatgcccaa cacttgtatg gccttgggcg ggtcatcccc ccccccaccc
    10381 ccagtatctg caacctcaag ctagcttggg tgcgttggtt gtggataagt agctagactc
    10441 cagcaaccag taacctctgc cctttctcct ccatgacaac caggctccca ggtcccgaaa
    10501 accaaagaag aagaacgcag atctctagag tcaccgagtt gctttaccgg atgaagaggg
    10561 ccgaaacata ctgtccaagg cccttgctgg caatccaccc aactgaagcc agacacaaac
    10621 agaaaattgt ggcaccggtg aaacagactt tgaattttga ccttctcaag ttggcgggag
    10681 acgtcgagtc caaccccgga ccggtgcatg tggaccccaa gaaaaagcgg aaggtggacc
    10741 ctaaaaagaa acgcaaagtg gatcctaaga aaaaaagaaa ggtgcacgtg tccatggtgt
    10801 ccaagggcga ggaactgttc accggcgtgg tgcccatcct ggtggaactg gatggcgacg
    10861 tgaacggcca caagttcagc gtgtccggcg agggcgaagg cgacgccaca tacggaaagc
    10921 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccttggcct accctcgtga
    10981 ccacactgac ctacggcgtg cagtgcttca gcagataccc cgaccatatg aagcagcacg
    11041 acttcttcaa gagcgccatg cccgagggct acgtgcagga aagaaccatc ttctttaagg
    11101 acgacggcaa ctacaagacc agggccgaag tgaagttcga gggcgacacc ctcgtgaaca
    11161 gaatcgagct gaagggcatc gacttcaaag aggacggcaa catcctgggc cacaagctgg
    11221 agtacaacta caacagccac aacgtgtaca tcatggccga caagcagaaa aacggcatca
    11281 aagtgaactt caagatccgg cacaacatcg aggacggctc cgtgcagctg gccgaccact
    11341 accagcagaa cacccctatc ggcgacggcc ctgtgctgct gcctgacaac cactacctga
    11401 gcacccagag cgccctgagc aaggacccca acgagaagag ggaccacatg gtgctgctgg
    11461 aattcgtgac cgccgctggc atcaccctgg gcatggacga gctgtacaag cctgcaggcg
    11521 agggcagagg aagtcttcta acatgcggtg acgtggagga gaatcccggg ccgtccaatt
    11581 tactgaccgt acaccaaaat ttgcctgcat taccggtcga tgcaacgagt gatgaggttc
    11641 gcaagaacct gatggacatg ttcagggatc gccaggcgtt ttctgagcat acctggaaaa
    11701 tgcttctgtc cgtttgccgg tcgtgggcgg catggtgcaa gttgaataac cggaaatggt
    11761 ttcccgcaga acctgaagat gttcgcgatt atcttctata tcttcaggcg cgcggtctgg
    11821 cagtaaaaac tatccagcaa catttgggcc agctaaacat gcttcatcgt cggtctgggc
    11881 tgccacgacc aagtgacagc aatgctgttt cactggttat gcggcggatc cgaaaagaaa
    11941 acgttgatgc cggtgaacgt gcaaaacagg ctctagcgtt cgaacgcact gatttcgacc
    12001 aggttcgttc actcatggaa aatagcgacc gctgccagga tataaggaat ctggcatttc
    12061 tggggattgc ttataacacc ctgttacgta tagccgaaat tgccaggatc agggttaaag
    12121 atatctcacg tactgacggt gggagaatgt taatccatat tggcagaacg aaaacgctgg
    12181 ttagcaccgc aggtgtagag aaggcactta gcctgggggt aactaaactg gtcgagcgat
    12241 ggatttccgt ctctggtgta gctgatgatc cgaataacta cctgttttgc cgggtcagaa
    12301 aaaatggtgt tgccgcgcca tctgccacca gccagctatc aactcgcgcc ctggaaggga
    12361 tttttgaagc aactcatcga ttgatttacg gcgctaagga tgactctggt cagagatacc
    12421 tggcctggtc tggacacagt gcccgtgtcg gagccgcgcg agatatggcc cgcgctggag
    12481 tttcaatacc ggagatcatg caagctggtg gctggaccaa tgtaaatatt gtcatgaact
    12541 atatccgtaa cctggatagt gaaacagggg caatggtgcg cctgctggaa gatggcgatc
    12601 tcgagccatc tgctggagac atgagagctg ccaacctttg gccaagcccg ctcatgatca
    12661 aacgctctaa gaagaacagc ctggccttgt ccctgacggc cgaccagatg gtcagtgcct
    12721 tgttggatgc tgagcccccc atactctatt ccgagtatga tcctaccaga cccttcagtg
    12781 aagcttcgat gatgggctta ctgaccaacc tggcagacag ggagctggtt cacatgatca
    12841 actgggcgaa gagggtgcca ggctttgtgg atttgaccct ccatgatcag gtccaccttc
    12901 tagaatgtgc ctggctagag atcctgatga ttggtctcgt ctggcgctcc atggagcacc
    12961 cagtgaagct actgtttgct cctaacttgc tcttggacag gaaccaggga aaatgtgtag
    13021 agggcatggt ggagatcttc gacatgctgc tggctacatc atctcggttc cgcatgatga
    13081 atctgcaggg agaggagttt gtgtgcctca aatctattat tttgcttaat tctggagtgt
    13141 acacatttct gtccagcacc ctgaagtctc tggaagagaa ggaccatatc caccgagtcc
    13201 tggacaagat cacagacact ttgatccacc tgatggccaa ggcaggcctg accctgcagc
    13261 agcagcacca gcggctggcc cagctcctcc tcatcctctc ccacatcagg cacatgagta
    13321 acaaaggcat ggagcatctg tacagcatga agtgcaagaa cgtggtgccc ctctatgacc
    13381 tgctgctgga ggcggcggac gcccaccgcc tacatgcgcc cactagccgt ggaggggcat
    13441 ccgtggagga gacggaccaa agccacttgg ccactgcggg ctctacttca tcgcattcct
    13501 tgcaaaagta ttacatcacg ggggaggcag agggtttccc tgccacagct tgatgaagat
    13561 ctgagctctt aattaatgat cataatcagc catatcacat ctgtagaggt tttacttgct
    13621 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
    13681 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
    13741 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
    13801 tcttatcatg tctggatccg tttaaactaa ctttaaataa ttggcattat ttaaagttac
    13861 atatggcggc caattcgcct tctgcaggag cgtacagaac ccagggccct ggcacccgtg
    13921 cagaccctgg cccaccccac ctgggcgctc agtgcccaag agatgtccac acctaggatg
    13981 tcccgcggtg ggtggggggc ccgagagacg ggcaggccgg gggcaggcct ggccatgcgg
    14041 ggccgaaccg ggcactgccc agcgtgggcg cgggggccac ggcgcgcgcc cccagccccc
    14101 gggcccagca ccccaaggcg gccaacgcca aaactctccc tcctcctctt cctcaatctc
    14161 gctctcgctc tttttttttt tcgcaaaagg aggggagagg gggtaaaaaa atgctgcact
    14221 gtgcggcgaa gccggtgagt gagcggcgcg gggccaatca gcgtgcgccg ttccgaaagt
    14281 tgccttttat ggctcgagcg gccgcggcgg cgccctataa aacccagcgg cgcgacgcgc
    14341 caccaccgcc gagaccgcgt ccgccccgcg agcacagagc ctcgcctttg ccgatcctct
    14401 agagtcgaga tccgccgcca ccatgattga acaagatgga ttgcacgcag gttctccggc
    14461 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
    14521 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
    14581 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac
    14641 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
    14701 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
    14761 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
    14821 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
    14881 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
    14941 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
    15001 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
    15061 tgtggccgac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
    15121 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
    15181 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
    15241 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
    15301 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
    15361 gatctcatgc tggagttctt cgcccacccc ccggatctaa gctctagata agtaatgatc
    15421 ataatcagcc atatcacatc tgtagaggtt ttacttgctt taaaaaacct cccacacctc
    15481 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
    15541 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
    15601 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatccct
    15661 aactttaaat aatgccaatt atttaaagtt aggaattaat tcacccagct ttcttgtaca
    15721 aagtggttga tatctctata gtcgcagtag gcggggccaa aaggaatgtg tatctataaa
    15781 acaatgatta tataaaaaga ggaaaatttc tattatcttg gggccaaact tttcatgaaa
    15841 taaaagagtg aaattcactt aacctaaaac aagataggaa agataaatca gtgcataacc
    15901 tttttacttt taaaataatt atatctccca cacagaatgt gtgctaccaa cagatataag
    15961 tttaaagccc ttcaccctaa aagctaccaa agaaactcct cccatcagac tgagaggaaa
    16021 tacactgaac gcgtatgggg atctgtccta atttactggt ctcattttgg acactaggaa
    16081 cctttacaca ctagttcccg tggagttaga tagtcgcctt tcattaacaa taaaagattt
    16141 tcttttctca gctatctgtg tttctggatg aaatttgtca tgagacggaa ctctctaaca
    16201 agtatggact ctcaggctgc tgcagccaaa gtggagtgga aagacatcag tgtctgctgg
    16261 cacgcaagaa gactgctccg gcctctgtcc cacccttcca gtttccagaa cctgccgaga
    16321 gttgcaaagc acatgaagaa aacagggcag tgttcatgaa caggtaagga ccatccttgg
    16381 gcatccagtg ggcagccagt gtttctgcag tccacatgtg cactccaaag tgtatacaag
    16441 tagggaatat agccagagta tgtctgctga ggtctgtgca ggggcttttg ttagacacac
    16501 actgtgagat gcagaacaca ccgcatgcag actcagcctc tgatccgtca gcctccggaa
    16561 acatagctct ttctcacctt tagagatggc gcaacgcaat taatgataac ttcgtatagc
    16621 atacattata cgaagttatg gtctgagctc gccatcagtt caagagccaa gtgttcatgt
    16681 acacccacga aactgataat taagaccatg cagtcagctt ctgggataaa tgtgcttgtc
    16741 acaaatgctt gcgggctttg atatcaccct ctacccttcc tttcaccttt aaaatagttc
    16801 attttatttt tgaaacaagg tcctagagga ccaggttggt cttgaactaa gtatgcatta
    16861 agggtgggtt tgagcttctt atcctcctgt ctctacctta caggtgccaa ggggacaggc
    16921 atatacatac atgcatacat acaacatata tttacatgtc tatctgtcta tactgataga
    16981 catactgaga gacagagaca gagaggacac cttagggtag tcatcgaact cttcactgat
    17041 aaagatttga gctgtcttac gtagaagcta gcctggctga ttccctgata tgtctgacag
    17101 gcttactgaa aaagacatct cagaaggaat catagcgtca ttgtttgttt tcataaaaga
    17161 ctctggatta acggattgga tctttttttt ctcctgaggc tgacatgtag gaacgttttg
    17221 acagcttttg aaaaaagcct gcacattttc ccgtaacagc acagtagaga aaaagtccat
    17281 tggaccctgt tgcctttgtg gttggttacc ccattcatta aataaattcc agagcttagt
    17341 tcagggaggt ggcagtccct tcatctgtta catgtttgca aatctccaag tgttgtccat
    17401 tccacaggtt catctatgaa gtgtcaagga ggaacccctt catgtatgcc ccagccattc
    17461 tgtccttggc tgctcagtac gacaaggtcg ttctggcatg ctgcaaagct gacaacaagg
    17521 aggagtgctt ccagacaaag gtggtatctt tgtgaagcta tctgaagcta aatggggttt
    17581 agccatggct tgggaaaaca gaattaaagg cagaaatgtg cctcctgtgg gaaataccat
    17641 cttctttctc atcctagatg aaagtcattt ttacttgatc ttttcaatta tcgatgaaat
    17701 ttcattttct atttatagtt agaaacaata ctccataata gtaaggtata actttaggcc
    17761 gaagtaaaaa tgtatttgga tcagcattta ctgaatcttt aaagagagca gagataacag
    17821 caatgcatgt acccagagga ggtgatttag tgcttataag cccattagag tgataggatt
    17881 gtccctctct ccttcagagc agaaaacaat gttgatacag agtaaccagc ctttcggtaa
    17941 ttgacaagtg gaaaacatac tacaagttca accagagcca aggaagctgg aaattagtgg
    18001 ggttgctaat gtgtccgcaa aatggagcat attccccact tctggcttgt tttattttgc
    18061 cttttgaggc agactcttgt tatatagccc caactgggcc caaactccta agaattatga
    18121 cggtagtgca attctttttt tttttttttc ttgtcttttc ctttcttttt aactgagcag
    18181 gagccaacag gctttacccc acctggagac tggacaggtg cagctgtcca tgtggtccca
    18241 gtatcaaaat gatgacgtat tggtttgaag atgtttaaga gatctcaggc atgcatacat
    18301 aagaattaat ctaaaagtca tgaaaataga atgacatgcc taaacgtcct gagctctaag
    18361 tttagtagaa gctattttat actttcctgt gtcatctgta ctgagcgttt ctagttctta
    18421 gatctgaaga gagaatgaat gggttttcta aacacctcac ttcttctgca tctagagagc
    18481 atccattgca aaggaattaa gagaaggaag catgttaaat gagcatgtat gttcagtgat
    18541 aagaaaattt ggatcccgaa acctccaggc aacgtaagta taagcgtcta aggaggcaat
    18601 gtgatgtgct caatgtcaat gtcactcctc cagagtaaag cctcctttgg gaggcagact
    18661 ctgattagaa tcatgagatc taaggatcag aacagaacta gcagatcagc ctgctattta
    18721 tgaaagtctc actggcatgg tattcaagga agagttttta agtaagtttt tattgtttgt
    18781 tctagacact tcagtttctg tccacactca aattatactg ccccattaga ccatatttcc
    18841 ccagctcttc tacaaagtag atcaagcttt atggagaggc atctccgact tgcagcctaa
    18901 taagaaatcc ttcttgttaa ccaggcttcc caatcaaccc cattgtgtca attgttgttt
    18961 ctcggtaaaa tattcccctg aggcaacaac tttagaagtc agtgacaggt gggcagaggt
    19021 ccttagtgcc tggaagccta ggtattgtgc acagccaaga tccaatgcac atttcagccc
    19081 tgtagtttct tccaaagact ggtttcacct ttcttctgga agaggtcaga cttatgctac
    19141 aattacctcc caattctatt agaaacttag tcatcaattc atatggcgcc ttaaatattt
    19201 gtcttaatag taaaatagaa atgggaggac acaatgacag caatggcatc atgtatctgt
    19261 tagcaagaaa agccacacac gccacaatga ttcccaagtt atcagtaaca aagactcaat
    19321 cctttattca aggaagcagg gtctttgcac aggcacctct tcccatgaat tctggcagct
    19381 ttgttttata cttccttctg ctgtggtctg atgccttgaa gcagaatctg gtagttcagg
    19441 ctggacttga gctcccgatt ctcctgcctc cacttctggt ttgctggaat tgtagccgag
    19501 tgcttctgtg tctgcaacaa acgctcagga tcagggctca acaagaaagg gcgacattta
    19561 gcttgagttc ctagaaagtc ttcaggaagc tacaaatgac ttccaaatta cttgagcacc
    19621 cagtccccca gtctacagag cttgtgcctg gtggagcaga agctgacgag acctaagatg
    19681 tgtttccgtc tcttttaaaa gaacacattt ctgttctttc ccgctggagg taactcccca
    19741 gatcctctac agacaatgcg atttcatcct gataaacaag ataatttttt tttaaatgat
    19801 cctcttccag aaccattatt aagctaagtc aaaagttaac tgaagcaaat tttactgaga
    19861 ttcagaagct ggccctggat gtggctcaca tccacgagga gtgttgccaa ggaaactcgc
    19921 tggagtgtct gcaggatggg gtaggagtct ccctctctcc ctttttgtct caattaatgt
    19981 ttcactatgc ctggcttggc tggggtgcag tgaacttaca gactttcgaa gagtaaaatt
    20041 tgagctacgt catgattgct ttggggtctc acctcaggca ttagcaatat ctgatccctt
    20101 tcatgagact acgagacatt cagttcttat ttgactgaga gactgagtct ctcccactag
    20161 ctagctagct gctggtttac agtgagggcc acttgccggg aaaacttata aactgacatc
    20221 aaagaatgta tagtttaact ggagagataa aacaattggc cttcttgaag ttatagactg
    20281 tcttttgttt accattgtct ctacagggca atgtgtggca cattgaaggg ttctgtcata
    20341 gtttaatgac agacttataa gtaaaaataa tgcaagaaac tgttgcaaga gagaaaatgt
    20401 ctatttatta ctaagcagag gtataaat
//