LOCUS       42446#pL1L2GT1_LF2A_nEGFPOT2A_CreERT2_pAbActNeopA#L3L4_pD223_DTA_T_spec        20969 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2GT1_LF2A_nEGFPOT2A_CreERT2_pAbActNeopA
COMMENT     design_id: 42446
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000047045
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            4438..4681
                     /db_xref="ENSEMBL:ENSMUSE00000421258"
                     /rank="2"
                     /note="ENSMUSE00000421258"
     exon            5441..5651
                     /db_xref="ENSEMBL:ENSMUSE00000233168"
                     /rank="3"
                     /note="ENSMUSE00000233168"
     misc_feature    4275..8924
                     /note="5 arm"
     misc_feature    8952..8976
                     /note="B1 Gateway"
     misc_feature    9943..9960
                     /note="FCHK QC critical region"
     misc_feature    9927..9942
                     /note="FCHK QC prefix region"
     misc_feature    9961..9966
                     /note="FCHK QC suffix region"
     misc_feature    10089..10140
                     /note="F2A"
     misc_feature    9966..10088
                     /note="1Dleader"
     primer          9812..9833
                     /vntifkey="27"
                     /dnas_title="seq primer FCHK2"
                     /label=seq primer FCHK2
                     /note="seq primer FCHK2"
     exon            9912..9965
                     /vntifkey="61"
                     /dnas_title="mouse En2 exon"
                     /label=mouse En2 exon
                     /note="mouse En2 exon"
     misc_feature    11012..11012
                     /note=" A to G to kill RsRII site"
     misc_feature    10962..11015
                     /note="self-cleaving' 2A peptide"
     misc_feature    11016..12041
                     /note="Cre"
     misc_feature    10146..10235
                     /note="3X NLS"
     misc_feature    10236..10952
                     /note="EGFP"
     primer          complement(9105..9126)
                     /vntifkey="27"
                     /dnas_title="LRPCR LAR3_EN2"
                     /label=LRPCR LAR3_EN2
                     /note="LRPCR LAR3_EN2"
     intron          9037..9911
                     /vntifkey="21"
                     /dnas_title="mouse En2 intron"
                     /label=mouse En2 intron
                     /note="mouse En2 intron"
     primer          complement(9001..9024)
                     /vntifkey="27"
                     /dnas_title="FRT3_oligo"
                     /label=FRT3_oligo
                     /note="FRT3"
     misc_feature    8989..9036
                     /vntifkey="21"
                     /dnas_title="Frt"
                     /label=Frt
                     /note="Frt"
     misc_feature    13302..13309
                     /vntifkey="21"
                     /dnas_title="polylinker"
                     /label=polylinker
     misc_recomb     13270..13301
                     /note="Rox(inv)"
     misc_feature    13262..13269
                     /vntifkey="21"
                     /dnas_title="RE sites"
                     /label=RE sites
     primer          13202..13220
                     /vntifkey="27"
                     /dnas_title="seq primer NF"
                     /label=seq primer NF
     primer          13076..13099
                     /vntifkey="27"
                     /dnas_title="LRPCR RAF5"
                     /label=LRPCR RAF5
     misc_feature    13019..13261
                     /vntifkey="21"
                     /dnas_title="SV40 pA"
                     /label=SV40 pA
     misc_feature    12051..12995
                     /note="ERT2"
     misc_feature    14509..14509
                     /note="G to C conservative mutation to kill RsrII site"
     misc_feature    14857..15100
                     /note="SV40pA"
     misc_feature    14915..14938
                     /note="RAF5"
     misc_feature    14664..14683
                     /note="neoBstB1_5"
     misc_feature    14371..14389
                     /note="neoSphI_5"
     misc_feature    14034..14053
                     /note="neoPstI_5"
     misc_feature    13865..14850
                     /note="neomycin resistance "
     misc_feature    13715..13734
                     /note="betact5"
     promoter        13319..13839
                     /note="human beta actin promoter"
     insert          13016..15095
                     /source="pL1L2_GTIRES_BetactP FLFL MapVerified"
     misc_feature    15096..15100
                     /note="for bActNeo"
     misc_recomb     15102..15133
                     /note="Rox"
     misc_feature    15146..15166
                     /note="B2 Gateway"
     misc_feature    8925..15196
                     /note="Synthetic Cassette"
     exon            15566..15773
                     /db_xref="ENSEMBL:ENSMUSE00000400031"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 1 ENSMUSE00000400031"
     misc_feature    15197..15927
                     /note="Critical Region"
     misc_feature    15951..15984
                     /note="Downstream LoxP"
     misc_feature    15928..16007
                     /note="synthetic loxP region"
     exon            20257..20400
                     /db_xref="ENSEMBL:ENSMUSE00000412236"
                     /rank="5"
                     /note="ENSMUSE00000412236"
     misc_feature    16008..20969
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggaattgtt agggctaagc tggtgacatg cagaattaag aaacattcag
     4321 tagtggggac actgatcagt ggctaatggt gacagaccta tgcgaggatg gagcgttctt
     4381 caggaatcca ctcccagtct tcagctaaca ctctcccctc ctctctcttc cctacaggca
     4441 tttcccactt gctaccagaa aataaagttt ttcaggaacc atgaaggttc tcctccactg
     4501 tcagagactt agattcatct ggcttgccaa gccagccggc cggcacttcc acagagattc
     4561 ccagctctgg gcacctctga cactggatga ctttgaagcc atcaatcgct gtgagaagcc
     4621 attacccaag aactttaact ttgcagcaga tgttctggac cagtggtccc taaaggagaa
     4681 ggtatcaaag agcagggccc cagtgttcct gttgctctca tctctgtctt ccaggtcctc
     4741 tcactgtgtt ctacctgtca agtttaaatc aattttttta tctcagtgag ggcaggaacc
     4801 tggatggctt catggagact cataagtaca agagcccata aacacctttc attccaatca
     4861 gtttctgtct tttgattctt ctcagaaaac ttagagtcta gggagatgaa ttcagattaa
     4921 ctcttccttg gtatatgctt atcccctctt taaaaagggc agaccccacc aagaggtgat
     4981 tctccaagac tgttctccag tgttccactg gagagaaaca tttctcccct aggcacactg
     5041 gggaagcagc tctggaggag tagcaataga cgtcctatct gccttctgtg ttagtcagtg
     5101 tgccatcact gtgacaaaac acctaagaca cacaatttca agtgaatgaa ggttgcttcg
     5161 gttcatgggt gcaacgatat cagcttcggt tcacttagcc ctgtggcagc cctgagcagc
     5221 acagggaaca tgcatggtga ggaagactgc ctgctcccct ccaggacagc tcagagaggt
     5281 ggaaggaatg agtgagagga agggcctagg gtctgatcgt tttcttcttt aagccctaac
     5341 gccacaggat aggggactat gccttttaac atgtgggtct tcaggtgaca gctctaaacc
     5401 ataatacatt atcttctctt tcccatgtgt tcctggccag tctggagaga ggccagccaa
     5461 cccagccctg tggtgggtga atgggaaagg agatgaggtg aagtggagct tccaagaact
     5521 gggttccttg tcccgaaagg ctgccaatgt gctcaccaag ccctgcggcc tgcagagagg
     5581 agaccgggtg gctgtgattc tgccccgtat ccctgaatgg tggctcatca atgtggcttg
     5641 catgagaaca ggtgagtatc acaaatgagt gtcaaaactt gagacttttg tgaagggtca
     5701 gctttgacta aagcaatcct tacaaaaagt agagaaagaa agttaacctt aatagcagtt
     5761 gtgatgaaat aataacaaca aacttaagaa attgggaaca ctgtgataac cattttttga
     5821 gagaggaaat caaagcacag tgatattaag acatttgcca aaggtcacat cacttgaaaa
     5881 ttatgtaatt ggggacctgg gaagatgact cagtggataa agtacttgct gtacaaacat
     5941 gtggccctgc attcccacat caaaagcttg gcatggaggg acaggtttct atgatcccag
     6001 cactgtgggc gcagagacag gcacatcccc tggtctcact ggccagccac acctagtcaa
     6061 gacagaaagt ctcaggttca cacacatata cccacacaca caaataaaaa tgaaaaagag
     6121 agttgcacta ttcagagttg cagaaaaaag ctctataaag caaatgcaag tgtgagtgtt
     6181 tctccacagc acagggtgtg tggaatatcc cccccataca cacacacaca cacacacaca
     6241 cacacacaca cacacacaca cgcgcgcaca cacgcgcgca cacacacaca cacgcacaca
     6301 cacggttctc tcttctgggt gtgtatctag attctctgca acacaaaaat acagaagaag
     6361 tggaagtctt tgaggatgca aagatttgta agaacataag ggactctgga cccaagtttg
     6421 gaaaaaaaaa aaaaactcca gtcagaagaa ctaatgtatc cagttacaca ttttccccaa
     6481 agtagtaaca tgtaaagtaa agacatgtct ttcagatagg ggtctgtgag ccatgaacta
     6541 tggttcctct gcttgggtaa atagacgtgt gatggaatac agctgactcc ttttgctcca
     6601 cagcaaacgt gctgactgag accaagaaca atcctacagg ccacagagtc taacacactc
     6661 tccaggcctt ttaccaaaag actctctgat ttcttatctt taaaaaaaaa aaatcatgcc
     6721 gggcatggtg gcacatgcct ttaattccag cactcaggag gcagaggcag tcagacttct
     6781 gagttcaagg ccagcctggt ctacaaagtg agttctagga cagccagggc tatacagaga
     6841 aaccctgcct cgaaaaacca acaaaaaatt aaaaaataaa aaaaatcatc agacatccat
     6901 taaaataaat aagaatgcca aagtgtagac tactacagag aacagaaaga tatctgtgca
     6961 atgttattag caggtataca aaaccaaact taaatagaaa tgattaaaca atgcataacc
     7021 tagctaaggt gtaagtttag ttgagttgtt gattgatggt tgaatggctt gatcttgatt
     7081 aaaaaataat ttatactagc aataataaca tcaggagaat ttcagagaat ttaagatgct
     7141 tttaatgcca ataaaataca tgaaagcagg agtataaaaa tgtttaaatt caccatataa
     7201 aaagacagag ataaattgga aatcagccgt ggttggaaga agattaagga tggtttatct
     7261 gttatgaaat atacccttaa tgtaatttaa agtaaattat ttaacttagg aaaatctgtg
     7321 agtacaaaca cacaagctag tgttcaaaaa catgaaataa aacaataaca acagttcatc
     7381 aagtgaagaa agccagctgc agacctgctt attccccaaa atgtcaaacc cttcatgtga
     7441 atttttaaaa ttgccttcac tcaccacata acaaactatt tgattttgct ggaaggcata
     7501 tgcaataaag tctaaaatac ttgccatcca acaaacattt gctgtctaaa acccattaga
     7561 agaaatcttc ctctgactcc caaggtggta atctactact gaacacattc ataaatccac
     7621 cacggagact gtaactggga atcattaaga gcaaataact tgtaagagaa atcattttat
     7681 atttttatat taattgatcg ttctgagact ctctaacgtg ttttcttaag ggaataactc
     7741 agctaactca gttaaaatat actcagtaaa atagccttgg gatggcaaga tggctctgca
     7801 ggtaaaggtg cttgctatgc aagtctggcc accagagctt ctgagtccat gagaccaacc
     7861 taaaggtgga aggagagcgt tgatattaca aaaggtgtcc tctgcccata tgcgtaccac
     7921 agcatgagag ctccccacaa taacactgat gacagtaaaa aaagagcctc cttcctagga
     7981 attcaactcc acaacttcct cctatttagc tgatttcaga atacccagca aggagtacat
     8041 acatagacat acaacacagg tcttgggagt gatagaaatg tctctgggga ctaaccttgc
     8101 tattggcatt gccatttatt ggctttgtga cccgtaggtg cgttatcatt cactggcatg
     8161 tgatatgtgc tctgtgcacg gcagtggcta tctgtaatgt tttgagtatc actgtcttcc
     8221 catggattca ctttagagag tcaaaccacc tgcttacatc ttatccttac atctaatcct
     8281 tacaccatac atgtaatcct gccctgtgca gtcttctcat aaggagcttt gaaatcctgt
     8341 ctctggctgc tgtttgggat gaagagggat aaattaagca tgaccatcgt catctaggaa
     8401 aatcttggcg gtaccagacc acggcatcaa tgatttgtag gctggctctg ccactcacca
     8461 gctatcccca aagactgcat gcacctcgtg gggatgctta aaacagtagt ttcaggttac
     8521 taaggtggct tggttactaa agatgcttgc tgcacgagcc tagctacatg agttagattc
     8581 ctaggaccca caaaaaggta caaggaaaga accacatcca caatggtatc atctgacctc
     8641 catgcaaaca acacacacac atacacacac acacacacac aaatgataga ttaaaaaaaa
     8701 tccttttaag tttttgaaga atagtctctg agcatctggg gctatgcaat ctatcaaaaa
     8761 aaattaatga gcaggcattt tcctagctgc atataatttt tcactctgtc ctgttgcact
     8821 gatgtagtat gcatcttaca aagcatttca acaaagagaa gaattctagt gtctctgtac
     8881 cttttacata tctattattt gcttttctca ttcccagata aattaaggcg cataacgata
     8941 ccacgatatc aacaagtttg tacaaaaaag caggctggcg ccggaaccga agttcctatt
     9001 ccgaagttcc tattctctag aaagtatagg aacttcgaac cctttcccac accaccctcc
     9061 acacttgccc caaacactgc caactatgta ggaggaaggg gttgggacta acagaagaac
     9121 ccgttgtggg gaagctgttg ggagggtcac tttatgttct tgcccaaggt cagttgggtg
     9181 gcctgcttct gatgaggtgg tcccaaggtc tggggtagaa ggtgagaggg acaggccacc
     9241 aaggtcagcc ccccccccct atcccatagg agccaggtcc ctctcctgga caggaagact
     9301 gaaggggaga tgccagagac tcagtgaagc ctggggtacc ctattggagt ccttcaagga
     9361 aacaaacttg gcctcaccag gcctcagcct tggctcctcc tgggaactct actgcccttg
     9421 ggatcccctt gtagttgtgg gttacatagg aagggggacg ggattcccct tgactggcta
     9481 gcctactctt ttcttcagtc ttctccatct cctctcacct gtctctcgac cctttcccta
     9541 ggatagactt ggaaaaagat aaggggagaa aacaaatgca aacgaggcca gaaagatttt
     9601 ggctgggcat tccttccgct agcttttatt gggatcccct agtttgtgat aggcctttta
     9661 gctacatctg ccaatccatc tcattttcac acacacacac accactttcc ttctggtcag
     9721 tgggcacatg tccagcctca agtttatatc accaccccca atgcccaaca cttgtatggc
     9781 cttgggcggg tcatcccccc ccccaccccc agtatctgca acctcaagct agcttgggtg
     9841 cgttggttgt ggataagtag ctagactcca gcaaccagta acctctgccc tttctcctcc
     9901 atgacaacca ggctcccagg tcccgaaaac caaagaagaa gaacgcagat cgcagatcgc
     9961 agatctctag agtcaccgag ttgctttacc ggatgaagag ggccgaaaca tactgtccaa
    10021 ggcccttgct ggcaatccac ccaactgaag ccagacacaa acagaaaatt gtggcaccgg
    10081 tgaaacagac tttgaatttt gaccttctca agttggcggg agacgtcgag tccaaccccg
    10141 gaccggtgca tgtggacccc aagaaaaagc ggaaggtgga ccctaaaaag aaacgcaaag
    10201 tggatcctaa gaaaaaaaga aaggtgcacg tgtccatggt gtccaagggc gaggaactgt
    10261 tcaccggcgt ggtgcccatc ctggtggaac tggatggcga cgtgaacggc cacaagttca
    10321 gcgtgtccgg cgagggcgaa ggcgacgcca catacggaaa gctgaccctg aagttcatct
    10381 gcaccaccgg caagctgccc gtgccttggc ctaccctcgt gaccacactg acctacggcg
    10441 tgcagtgctt cagcagatac cccgaccata tgaagcagca cgacttcttc aagagcgcca
    10501 tgcccgaggg ctacgtgcag gaaagaacca tcttctttaa ggacgacggc aactacaaga
    10561 ccagggccga agtgaagttc gagggcgaca ccctcgtgaa cagaatcgag ctgaagggca
    10621 tcgacttcaa agaggacggc aacatcctgg gccacaagct ggagtacaac tacaacagcc
    10681 acaacgtgta catcatggcc gacaagcaga aaaacggcat caaagtgaac ttcaagatcc
    10741 ggcacaacat cgaggacggc tccgtgcagc tggccgacca ctaccagcag aacaccccta
    10801 tcggcgacgg ccctgtgctg ctgcctgaca accactacct gagcacccag agcgccctga
    10861 gcaaggaccc caacgagaag agggaccaca tggtgctgct ggaattcgtg accgccgctg
    10921 gcatcaccct gggcatggac gagctgtaca agcctgcagg cgagggcaga ggaagtcttc
    10981 taacatgcgg tgacgtggag gagaatcccg ggccgtccaa tttactgacc gtacaccaaa
    11041 atttgcctgc attaccggtc gatgcaacga gtgatgaggt tcgcaagaac ctgatggaca
    11101 tgttcaggga tcgccaggcg ttttctgagc atacctggaa aatgcttctg tccgtttgcc
    11161 ggtcgtgggc ggcatggtgc aagttgaata accggaaatg gtttcccgca gaacctgaag
    11221 atgttcgcga ttatcttcta tatcttcagg cgcgcggtct ggcagtaaaa actatccagc
    11281 aacatttggg ccagctaaac atgcttcatc gtcggtctgg gctgccacga ccaagtgaca
    11341 gcaatgctgt ttcactggtt atgcggcgga tccgaaaaga aaacgttgat gccggtgaac
    11401 gtgcaaaaca ggctctagcg ttcgaacgca ctgatttcga ccaggttcgt tcactcatgg
    11461 aaaatagcga ccgctgccag gatataagga atctggcatt tctggggatt gcttataaca
    11521 ccctgttacg tatagccgaa attgccagga tcagggttaa agatatctca cgtactgacg
    11581 gtgggagaat gttaatccat attggcagaa cgaaaacgct ggttagcacc gcaggtgtag
    11641 agaaggcact tagcctgggg gtaactaaac tggtcgagcg atggatttcc gtctctggtg
    11701 tagctgatga tccgaataac tacctgtttt gccgggtcag aaaaaatggt gttgccgcgc
    11761 catctgccac cagccagcta tcaactcgcg ccctggaagg gatttttgaa gcaactcatc
    11821 gattgattta cggcgctaag gatgactctg gtcagagata cctggcctgg tctggacaca
    11881 gtgcccgtgt cggagccgcg cgagatatgg cccgcgctgg agtttcaata ccggagatca
    11941 tgcaagctgg tggctggacc aatgtaaata ttgtcatgaa ctatatccgt aacctggata
    12001 gtgaaacagg ggcaatggtg cgcctgctgg aagatggcga tctcgagcca tctgctggag
    12061 acatgagagc tgccaacctt tggccaagcc cgctcatgat caaacgctct aagaagaaca
    12121 gcctggcctt gtccctgacg gccgaccaga tggtcagtgc cttgttggat gctgagcccc
    12181 ccatactcta ttccgagtat gatcctacca gacccttcag tgaagcttcg atgatgggct
    12241 tactgaccaa cctggcagac agggagctgg ttcacatgat caactgggcg aagagggtgc
    12301 caggctttgt ggatttgacc ctccatgatc aggtccacct tctagaatgt gcctggctag
    12361 agatcctgat gattggtctc gtctggcgct ccatggagca cccagtgaag ctactgtttg
    12421 ctcctaactt gctcttggac aggaaccagg gaaaatgtgt agagggcatg gtggagatct
    12481 tcgacatgct gctggctaca tcatctcggt tccgcatgat gaatctgcag ggagaggagt
    12541 ttgtgtgcct caaatctatt attttgctta attctggagt gtacacattt ctgtccagca
    12601 ccctgaagtc tctggaagag aaggaccata tccaccgagt cctggacaag atcacagaca
    12661 ctttgatcca cctgatggcc aaggcaggcc tgaccctgca gcagcagcac cagcggctgg
    12721 cccagctcct cctcatcctc tcccacatca ggcacatgag taacaaaggc atggagcatc
    12781 tgtacagcat gaagtgcaag aacgtggtgc ccctctatga cctgctgctg gaggcggcgg
    12841 acgcccaccg cctacatgcg cccactagcc gtggaggggc atccgtggag gagacggacc
    12901 aaagccactt ggccactgcg ggctctactt catcgcattc cttgcaaaag tattacatca
    12961 cgggggaggc agagggtttc cctgccacag cttgatgaag atctgagctc ttaattaatg
    13021 atcataatca gccatatcac atctgtagag gttttacttg ctttaaaaaa cctcccacac
    13081 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
    13141 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
    13201 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
    13261 cgtttaaact aactttaaat aattggcatt atttaaagtt acatatggcg gccaattcgc
    13321 cttctgcagg agcgtacaga acccagggcc ctggcacccg tgcagaccct ggcccacccc
    13381 acctgggcgc tcagtgccca agagatgtcc acacctagga tgtcccgcgg tgggtggggg
    13441 gcccgagaga cgggcaggcc gggggcaggc ctggccatgc ggggccgaac cgggcactgc
    13501 ccagcgtggg cgcgggggcc acggcgcgcg cccccagccc ccgggcccag caccccaagg
    13561 cggccaacgc caaaactctc cctcctcctc ttcctcaatc tcgctctcgc tctttttttt
    13621 tttcgcaaaa ggaggggaga gggggtaaaa aaatgctgca ctgtgcggcg aagccggtga
    13681 gtgagcggcg cggggccaat cagcgtgcgc cgttccgaaa gttgcctttt atggctcgag
    13741 cggccgcggc ggcgccctat aaaacccagc ggcgcgacgc gccaccaccg ccgagaccgc
    13801 gtccgccccg cgagcacaga gcctcgcctt tgccgatcct ctagagtcga gatccgccgc
    13861 caccatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
    13921 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
    13981 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
    14041 actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
    14101 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
    14161 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
    14221 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
    14281 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
    14341 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc
    14401 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
    14461 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggccg accgctatca
    14521 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
    14581 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
    14641 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc
    14701 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
    14761 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
    14821 ttcgcccacc ccccggatct aagctctaga taagtaatga tcataatcag ccatatcaca
    14881 tctgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
    14941 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
    15001 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
    15061 ttgtccaaac tcatcaatgt atcttatcat gtctggatcc ctaactttaa ataatgccaa
    15121 ttatttaaag ttaggaatta attcacccag ctttcttgta caaagtggtt gatatctcta
    15181 tagtcgcagt aggcggatta aatatcacac aatcattaca caatcacctt tcaagcctga
    15241 catgaaagtg gttcttacta ctcagcccat cattgtcaac cagctgctgt cattatcccc
    15301 atgataaaag taaagaaggg aaggccagag aaacccaagt catagggcta gcaagttgca
    15361 agaccaagac tagacccaag gcctgtcaga ttccaaagcc agagctctac tgatccctca
    15421 tgccagggcc cagcaggaca gcccagggaa ccaagttaca ggtagctgag ggctgacatc
    15481 tgaagttctc attcatccag acacaagacc tagatttccc aataatctcc agagctgtta
    15541 gctcaggaat cactttttgt tccagggctt gtattcatgc caggaacgat ccaactgacc
    15601 agaaaagaca tcctctaccg gctgcaagca tccaaggcca agtgcattgt ggctagtgag
    15661 gaagtggccc ccgcagtgga ctccattgta tcagagtgtc ccagcctgaa gactaaactc
    15721 ctggtgtctc cacaccactg ggatggatgg ctcaactttc aggagttact tcagtgagta
    15781 tttgggtggg aggcactagc aacagcacaa atcacaggta cagctctgaa tctgtccgtc
    15841 caaagactca gcaatagcca gagttgatac cgggctcctt cccaaaacag aatgttttta
    15901 agagtatgag cttatgagta catgtgtgag atggcgcaac gcaattaatg ataacttcgt
    15961 atagcataca ttatacgaag ttatggtctg agctcgccat cagttcagaa catatgtcct
    16021 ctgtaataat aataggtact cttagaccct agaccatctc tccagctccc taaaaagtca
    16081 tttaatgaaa ttatttacag acagaagaaa tatgtacagc tctactctga cttttctgtc
    16141 tcctctgtgg cctatgatga gtgtgctata gagatgggcc tttgggacac aactatcatg
    16201 atctctcttt tactctttga acatgctcta gctcagaacc ttggcatcca ctgtctctcc
    16261 tgccccgatg ttctttccta gtcctttcta tagttgttct atcttgtcag gtttcagttc
    16321 aaatgtttta tcctacgtga tgctgctcct gaccatcttg atggaagccg taaggctttt
    16381 ttctccatgc atggactgtt tcttcgcgtt gctaggtcct ctgagtgatc agactagcct
    16441 cgtgttcttt tagattcagt ttttctactc caaggtttgg actggaaaat taggctccaa
    16501 agccaaatcg ggtccaccac ttgcttttgt aaataaagtg tcattggcac acagaggtgc
    16561 tcatttgttt gtatattgtc tatgtctgct ttcatttaga aatggtatgc ctgctatctg
    16621 gccctttgca gaaagcatga cagccgctgc cttaagttaa actctctaag tatgccaagg
    16681 gttttcttgt gtgttcgggt gataccttta tcttcaacac ataacaaaac acagctgggt
    16741 gctgaaggaa tagttaataa gcagacaaaa aaaaaaaaaa cagatcactt aacctctgtt
    16801 ttcttcatat gtaaaatggg gataactata ctgcaagtat ctagtgacat ttgacagcaa
    16861 aataagctaa tacatgagaa agaatttccc atgttatttt aaaaagtgag aaaatgttca
    16921 ttgacagcat aatccttatt aaaatagcag atgttttata aaaataaaaa aaatccagct
    16981 tatttttttt gtaagttata aactcaggta agctatgtga catttctgaa aatctgcatc
    17041 ttaatctatg agatcaggat aatgacagta tggggttgca gaaggaacaa acgggataaa
    17101 gcaatgtgac atgctcggca taatggctag aacaaatggc acagtagttg gaactgtgtt
    17161 tgaggctgac tcacatggca gacaaaggag cagttgaggc ttggttgact tttggttggg
    17221 agataggtgg ctgggtactg tcattttgga gaagggtgga tgggtactgt catttgagga
    17281 aagatgagac cagtgataca gcttgtcagc acggtacttg gtactgactg aatgacaaga
    17341 ccttggtctc cactctcagt gctacaaaat gaaagctaaa tgtagacatg gggtatattc
    17401 agaaggtcaa agcaggagaa tgaagaactt agaagccagt ttaaggtaca caacaagata
    17461 ctatctaaaa agaaggaaaa taaaaataaa acaggcaggc aggcagacaa gaaggaagga
    17521 aggaagggag ggggacgggt acggggatgg agacggatgt agagcaggaa agaaccagca
    17581 gagactgcta taagatagta agcttagttt taggggctgt taagagcact gactattctt
    17641 ccagaggacc caggtttgag tcccagcacc cacatggcag cccacaacca tctgtaagcc
    17701 aagttctaaa tgttccaatg cccaattctg gccttcatgg gcactgcatt tatggggtac
    17761 acagacatac atgcaggaaa atcacacatt tttaaaaaga caataagcca gtcatatgga
    17821 gatcagggat ctaggaagat ccaggtgtct aacaagtgtg gaatatgtga gtctcaggta
    17881 taatgataaa aatcttgaag ttaccaggaa aaagttatct ctgaaaattc agaactcaaa
    17941 acttagggag ccaaaaagaa agaaaaccca gacttagcat caccccatga atatctttct
    18001 gcctctcaga gactccaatg tctttgtttt ttcagggaaa agtaaacaca tggatgaagc
    18061 cttgtgttag taggtcctct tacttaatgg agaaacacca aagacaagag atgtccccta
    18121 agctaggatg ttagataaag taaactaatc acactctccg gaacctaaaa catccaacat
    18181 cacccaatat ttgttctgaa agaaaaaaaa caaaactcaa tgatcttttc tattttaagg
    18241 aaacagagtt gctaaaaata tctcaatatt ttgagaatcc atttttggca ttatggactg
    18301 aggatgttta ttacctaatg atctctatct tagtgccatc tcgatcaatg gcattcactc
    18361 atcattacaa tcatctgaat gttagagatg tacttcagat gatagaattt gcctagcatg
    18421 tgtgcagctc cagacttctc tagagccttg taacttgaat gtggtggtac aagcgtataa
    18481 tctcagcacc caagaagtag cagcaagagg aagtttaaca tcatcctcag atgcaatttt
    18541 cagccagtct gggtcttatg agataatgtc tcaaaataaa atgataacat aaagttcact
    18601 gaaataaaaa cctatcactt ggaattttcc aaaatgagaa atttgaaaaa tataatacat
    18661 gaggcataca gatagatgta atacagagca caatgatgca taccattcat cccagcatcc
    18721 aggaggcaag gcaagcagat atcatcaatg agtttaaggc cagactggtc tacacgatgt
    18781 tccaggccag tcatgatcta taatgtctac cagaagaagt atgtcagttc acctcatgtg
    18841 gccttctttg tcacacaaga caatctgaat cccagaatcc ctgccacaaa atgtacacca
    18901 gaataagtca taagcctcac ccagatcctt cagtgtggga atggcatgtt tgtccaggga
    18961 tgggaggaat tggaaagctg tgtacaccaa aagtcttttt ctgtcaccta agatacacac
    19021 tttccacctt agtaatccaa gtacagatga aacatacagt actgaggggt gtctaggtgg
    19081 ttcgatgaaa caccaacccc atctttgtta tgatgccagt atgactagga gaattccaga
    19141 catgtacatt gtatacattc tatacatgga caggtatcat gggttgtgat gaaagatgaa
    19201 tgggatggga gacagacaat ggcatcctgt cttcattact cctttttttc ccatgaaggt
    19261 gtgtgtgtgt gtgtgtatgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgttgccat
    19321 gtgtgtaggt acatatatat gtaagagtac acatgtacat gtttgcatgc aaaggcctga
    19381 ggctgatgtc aggtgtcttc ctcgatctct ctgccttact cattgagtca ggagctctcc
    19441 tcaaacccag agcccatcaa tgtcactagt cttgtgctag ggaatcaaat ctgacctttc
    19501 aagcaaagga atcacgatgg gccaccacgg ctccctggca tttacttggg tgctgggata
    19561 caaactttgg tcctcatcta cagcccacat cactcttctt aaaacaagaa aatgtctcct
    19621 gtaggacttt atacctgttg actataattc tcccaagcct attcctaaac caatcactgg
    19681 aaagggaaaa aaccatcctg attggcttag cttaattgtg actaagtgag aagcatgtgg
    19741 ctacctagta cctgaacaaa acctgacttt tgttagttca gtaaaaggat gggatggcta
    19801 tttggtccgc tattagctgt ttctgtcaga gatactgaaa taatggacaa taacaacaaa
    19861 ggttttaagt ttgatctcaa tatatctggc ctttaaatct aaaggtcttg ctctaatccc
    19921 taaatcatga aatggtatca acttagaaag actcaaatct gtttttaatt tttttatata
    19981 aattacaaag acattgaaat aaagtatatg tgcttctgtt attgacatga cgagttacct
    20041 tggctgtctt tttggactac aaagaaatga gcttaaaaag gaggacttta ccctgatctt
    20101 tagagtccct ctaagattct tgggagtttc tgctcaggga agctctgcag taggttcagt
    20161 tcattattcc cccgtggtaa gtgggaccct agtagagaac aatcatacag agtcaactga
    20221 tctcccctca gtctatatct tggggttcat ctgcagatcg gcctctgaag agcacaattg
    20281 tgtggagacg ggaagtcaag aaccaatggc catttacttc accagtggga ccacaggctc
    20341 ccccaagatg gctcagcact cacagggcag ccttggcatt gggtataccc tttgtggaag
    20401 gtaggaaagg aaactatggt tttgttctta ttttcattgc agcctggcct cacactgtag
    20461 ttggctctgg actataactc actctgtacc cttgactggc ctcaaattca tagcaattct
    20521 cctgcctctc ttccctcccc cacaaaaata gttgaatttg caggagtgca ccaccacact
    20581 gcttctttgt tttctatgca gtctcccagg ctgccagtgt gggtgtccaa ctgctgatat
    20641 gacacctctc cttgaatgga tattaagata tctttaacgt agatccataa aacagaactc
    20701 tggatgcctc taccctaccc tagctaaatg gtaccattga agtagctgat caagcaagaa
    20761 tgtaggttta gtgcttgatt catagcactt ctgcctctct ctctctcctt atttccaggt
    20821 gaatttacct tcccaaaagg gtctttgaaa caagtctacc taaaactgcc acattgctct
    20881 gtttaatttt ccttctacct aaagttaaag cattcaatga ttactctcaa tcatcaaccc
    20941 aataaagtct agaataccca agggattgt
//