LOCUS       199013#pL1L2GT0_LF2A_nEGFPOT2A_CreERT2_pAbActNeopA#L3L4_pD223_DTA_T_spec        20605 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2GT0_LF2A_nEGFPOT2A_CreERT2_pAbActNeopA
COMMENT     design_id: 199013
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000090166
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            8663..8884
                     /db_xref="ENSEMBL:ENSMUSE00000635627"
                     /rank="1"
                     /note="ENSMUSE00000635627"
     exon            9066..9326
                     /db_xref="ENSEMBL:ENSMUSE00001072604"
                     /rank="2"
                     /note="ENSMUSE00001072604"
     misc_feature    4275..9829
                     /note="5 arm"
     misc_feature    9857..9881
                     /note="B1 Gateway"
     misc_feature    10848..10851
                     /note="FCHK QC critical region"
     misc_feature    10832..10847
                     /note="FCHK QC prefix region"
     misc_feature    10852..10857
                     /note="FCHK QC suffix region"
     misc_feature    10980..11031
                     /note="F2A"
     misc_feature    10857..10979
                     /note="1Dleader"
     primer          10717..10738
                     /vntifkey="27"
                     /dnas_title="seq primer FCHK2"
                     /label=seq primer FCHK2
                     /note="seq primer FCHK2"
     exon            10817..10856
                     /vntifkey="61"
                     /dnas_title="mouse En2 exon"
                     /label=mouse En2 exon
                     /note="mouse En2 exon"
     misc_feature    11903..11903
                     /note=" A to G to kill RsRII site"
     misc_feature    11853..11906
                     /note="self-cleaving' 2A peptide"
     misc_feature    11907..12932
                     /note="Cre"
     misc_feature    11037..11126
                     /note="3X NLS"
     misc_feature    11127..11843
                     /note="EGFP"
     primer          complement(10010..10031)
                     /vntifkey="27"
                     /dnas_title="LRPCR LAR3_EN2"
                     /label=LRPCR LAR3_EN2
                     /note="LRPCR LAR3_EN2"
     intron          9942..10816
                     /vntifkey="21"
                     /dnas_title="mouse En2 intron"
                     /label=mouse En2 intron
                     /note="mouse En2 intron"
     primer          complement(9906..9929)
                     /vntifkey="27"
                     /dnas_title="FRT3_oligo"
                     /label=FRT3_oligo
                     /note="FRT3"
     misc_feature    9894..9941
                     /vntifkey="21"
                     /dnas_title="Frt"
                     /label=Frt
                     /note="Frt"
     misc_feature    14193..14200
                     /vntifkey="21"
                     /dnas_title="polylinker"
                     /label=polylinker
     misc_recomb     14161..14192
                     /note="Rox(inv)"
     misc_feature    14153..14160
                     /vntifkey="21"
                     /dnas_title="RE sites"
                     /label=RE sites
     primer          14093..14111
                     /vntifkey="27"
                     /dnas_title="seq primer NF"
                     /label=seq primer NF
     primer          13967..13990
                     /vntifkey="27"
                     /dnas_title="LRPCR RAF5"
                     /label=LRPCR RAF5
     misc_feature    13910..14152
                     /vntifkey="21"
                     /dnas_title="SV40 pA"
                     /label=SV40 pA
     misc_feature    12942..13886
                     /note="ERT2"
     misc_feature    14988..15570
                     /note="synthetic fragment mutates RsRII"
     misc_feature    15400..15400
                     /note="G to C conservative mutation to kill RsrII site"
     misc_feature    15748..15991
                     /note="SV40pA"
     misc_feature    15806..15829
                     /note="RAF5"
     misc_feature    15555..15574
                     /note="neoBstB1_5"
     misc_feature    15262..15280
                     /note="neoSphI_5"
     misc_feature    14925..14944
                     /note="neoPstI_5"
     misc_feature    14756..15741
                     /note="neomycin resistance "
     misc_feature    14606..14625
                     /note="betact5"
     promoter        14210..14730
                     /note="human beta actin promoter"
     insert          13907..15986
                     /source="pL1L2_GTIRES_BetactP FLFL MapVerified"
     misc_feature    15987..15991
                     /note="for bActNeo"
     misc_recomb     15993..16024
                     /note="Rox"
     misc_feature    16037..16057
                     /note="B2 Gateway"
     misc_feature    9830..16087
                     /note="Synthetic Cassette"
     exon            16474..16609
                     /db_xref="ENSEMBL:ENSMUSE00000586494"
                     /type="targeted"
                     /rank="3"
                     /note="target exon 1 ENSMUSE00000586494"
     misc_feature    16088..16766
                     /note="Critical Region"
     misc_feature    16790..16823
                     /note="Downstream LoxP"
     misc_feature    16767..16846
                     /note="synthetic loxP region"
     exon            20129..20605
                     /db_xref="ENSEMBL:ENSMUSE00000586493"
                     /rank="4"
                     /note="ENSMUSE00000586493 5' fragment"
     misc_feature    16847..20605
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggataaaat cacgaatgtg aaactatgta gcccagcttt agggagtgta
     4321 ctctgcctct agacttgaag atgtaccata tttttctctg aaagactcaa taattttata
     4381 accaggtatt tttaatacat gcacacagta tattttggtc atattcactc cactcttacc
     4441 ttaactcctc atggcatcac cgcccacctc cccatcctct ccctgcatca tgcccgtttc
     4501 tttcttaaca ccccacaggg tcaggtttat gctgctccaa tgtgcatggt gtggagccat
     4561 cccctggggc acagttgacc tgccaaggat cataccccta aaggaaactg tctcaactag
     4621 gcatgaaggc gtgtgaaccc tgcttcctgg gatgttaaat ggcttgacct tgtgcatgca
     4681 gccacagctg ctgtgagtac agtggcggtg atgtccaggg gacactgttt ctccctggtc
     4741 ttcctcaatc tctgggttct cacaatcttt ccaacccccc ttccaaaata ttgcccagcc
     4801 ctggggagct gggtgacact gatgtcccat ttatggaaag atgtgtaaca cacactatct
     4861 gcaccttaac caattgtggg tttcttcatt aactgtcatc cacagcagaa agaggtgtga
     4921 gttgcagtaa tctacgagtc aatttttatg tctttcgagg acagtttggt atgatgtcta
     4981 gttagcaata aaacagtagt aacaggttta cctctggagc ctatgcgctc cctcccagtc
     5041 atgggttttt ggccagagtt atggtgccag gcacatttcc catttgtgga gtgggcctta
     5101 aatgcaatca gaaagtggtt gatcagcccc atagcatgtc actacgacac ccatgggcac
     5161 atcccaccag tctggtcatt gctgcagctt gcagagttca gaggtaaaac tgttggtgac
     5221 ttttttctcc cagttccaca catagagccc tccagtgcta caaaagtaag ccagcgggga
     5281 gaaagcatcc cagttagaac cagcttaatt tccttctcca gttaaagcat ttcttccctc
     5341 tcccactagt ggcttctttt cattttcctg atgtgttagt taggatttct attgctgtga
     5401 taaaacacca gaatcaaaag caatttggga aggaatggct ttatgtcatc gtcccactct
     5461 cagatgacac tgcatcactg agtgaaatcg gggcaggaat taaaggcagg agctgaagca
     5521 gaggccacag aggagagcca cttagtcact cgccccgccc actaatggct tgctcatcct
     5581 gctttcatat acatctcagg accagcccag gattgcactg cccacagtat acaggaccct
     5641 cccacctcag ttaccaagac aacacaccca cagactttaa tacacaagcc catctgttga
     5701 aaaacatctt tttaattaag atgttcattt ccttgttata tctaggttac atccagttga
     5761 taaaaaccaa cccatacacc caactctaca actttccatg ttaaacatgc aaaactaaaa
     5821 atttggtgcc aagatccaca tattagtgag aacacaatga gtttgttttt ctgacaccag
     5881 gcattttata tccaattatt ttttttcatc tctcagatta tagcctctgg gatctgtggc
     5941 actgataatc ataccttaga agggaaaatg aaaacgccat ttcctgtcat cctgggccac
     6001 gagggagctg gagttgtgga gagtgttgga ccaggtgtaa ccactgtaaa gccaggtggg
     6061 taacgaggca tgatactgat gctatctagg ggtgcaggaa tgggttcagc ttctctgaca
     6121 attaaaacat taacagtcag gagaacggtg ctcccagaaa tctcggggag tcccagcaga
     6181 gccgtcatgt gatacctgag aagtcagggt ccctctggat atgtttagtt ttattatcag
     6241 aaaaaattca agagcaatcg caagcaagca tggggacaaa gattggactc aaatggaagt
     6301 gtgaagacag tttattggag cctaaaaggg aaaccgcagg tcaggcaagc tgggagggag
     6361 aggaggagtg gagaagagaa ggaacaaaag tcagtttgct tgtgctgagg ccgtctggag
     6421 gtcagcctcc tggtcgcaag tcaaggagga acgctttagg ataaagagca gggaacccta
     6481 acgcattaaa gtatcgagtg catgagcatg tataggctat gggtggccag catctgagag
     6541 aaaaagaaga aatagtcagg tcttcctagg ctggcctcag accccaagtc tcaggatggg
     6601 ttagctggta gggatagcat tatctttcag gccttctgga gaggaaaagc atattacctt
     6661 tgggtgaacc tttgggtgcc ctgtgtatta gctcttgtag gatcagacaa gggcctatct
     6721 ctgtatagag gcagattcca ttctttacaa tatagcccca gttcctttcc taattcctac
     6781 tctgattctt cccccaaggt aaaataaaga tctcagaact caagccaggt gtggtggtgc
     6841 atgcctttga tcccagctct tgggaggcag aagcaggcag atctctaagt gttagaggct
     6901 agcctggtct acagagtgag taccaggaca gccagggcta aaatccaaaa acaaaacaaa
     6961 acaaaacaaa acaaaaaaat ttaaagctca tcacatattg acaaaaccat caaacttcat
     7021 ccagacatca gatatcgtac aagatatagg cagcctggga tccagaatcc cagactgaag
     7081 gcggagttca gtggtgtgac acttgcctac catgcacaag actgtgttcc tcacaccagt
     7141 ctattggaaa ttaaactaag aattgcaata tatacaatgt atcagaatat tggtaaacat
     7201 agaatttgtg tggttacaga gcccttttac acacacacta tgtgtcctaa tctttttaag
     7261 tgtgcagagt tgcatcgtgt gtaagaattt cttctagaat ggatatttac ctcccccatg
     7321 ctccagacct tcaggtttca aacacttgat aatgtcatta tgattatcat tgccatagat
     7381 atttttgttt tccttcatgt gtactgtgag catgctatgt cagagagatg ggtgaacata
     7441 tttgtttctt tccacaatgt cagaatatac agaataacaa tatgttcaca tgatatagca
     7501 gttttcttgg cagcaatttg ctagacattg ttgcctacct tgctatcaag cactggctgt
     7561 tgattcccgt cctcgttact aatctgaact ctctgatgac atgttacaca tcacacagta
     7621 aatgcatttg tttgtgtgtg gaagagtacg gactacagaa tgagtagata tgtaggctgc
     7681 agcagcaaga aatttctagg gacctaagag gcaaagctgg aaaactgaag gaaatgcaaa
     7741 taaatagagg ctttgggaag ggtgaggtct accagatggt gcacttcctg tgttctcttg
     7801 ggagcagagt catccctctc cattctcaaa aaggagtcaa aagctacttg taaagtcacg
     7861 tctttggggg caatcaatct gaaagccccg ttttatagag ggcctcaagg caggacacag
     7921 acagatctca accatgtata aagaaataga tactctgaaa aagatcaatt accttgttta
     7981 cctaatttag ctaaacaggg ttaaaggcac aagctgtctt ttctcaacat tacaatacct
     8041 cataatgagt actgatttta atttagctca tgtataattc attcttccaa taaatattga
     8101 attctactaa ggtctaattc tacgtttctc ctggaacatt gggccaaata gaaaagtctt
     8161 ttatatatca tcaatattaa ggtcactctt acatttaact atctcttacc tttgaacctc
     8221 aatgtcatga gatgtaaata cccaagttct tttttatctt gcagatctat tatattaaaa
     8281 ttgattgttt taatctcatg gtgaaatgat agttaacttc aagtaaaaga ctccatgcaa
     8341 ctcttcttat gacaaattga tgacacctgc caaaaacagt tcccagtagt attaactgta
     8401 cttcaggcct gtcatgactt tccccataac tttctttatt ctttttcttc aggagataaa
     8461 gtcttaatgt tccctcttcc ggaatgtcga gaatgcttct actgcctcta ccctaagggc
     8521 aacttctgtg agaaagaaaa gttagtttca atgtctttga cttctcacct cattcccttt
     8581 cctcacacag ggtttggcac aaccctgata atgtctgggg ttttttttgt tttgttttgt
     8641 tcttttcctg ttgttgttgc tcagtattct ctctccaact ggattaatgt tggatggaac
     8701 cagcagattt acctgcagag ggaagaaaat ctataatata atggggacca gcacgttcac
     8761 ggaatatact gtggtgcatg agattgcagt ggcgaagatc gaggctgctg cccctatgga
     8821 tacagtctgc atcatgagct gcgcggtgcc gacaggcttt ggagctgtgt ttaacacagc
     8881 tcaggtgagt catcgttctt cctgtgttct ttccagtgac tgcctacata gtcaaggagg
     8941 agccacatag ctggaaggct ccttcatcat ggacaaaacc gaagggtttt ccccctctct
     9001 ttattcccta caaatacaaa atcactattg aaagtctaac aaaggtttat cctttaaaaa
     9061 tacaggtcac ccctggctct tcctgtgtgg tgtttggact cggaggaatt ggctctgcca
     9121 tcgtcatggc ctgcaaagcc tctggagcat gtagaatcat cggggttgac atcaatgagg
     9181 agaaatttcc tcgggcaaga gccttggggg tcaccgactg tctcaaccct aacaagctga
     9241 agaagcctgt gcaggaggtg gtgaaggaaa tgacaggggt tggtgttgac tttgcctttg
     9301 aggccatcgg actcattgaa accatggtgt gtgtcttggg tacaatgggg gcataacaca
     9361 ctaacacatt agcttcagaa agaaggaatt ttcctgtaat ttgttttatt tgggaggagt
     9421 gttgcaaatt gagcccagag cctctagcat accaaacata attctatacc tctgtagtcc
     9481 taatgtaggg ctataaattt ttccctagca tttttgagca cactgatatg ccttgcattg
     9541 ttctgagcta ctagcctatt ctatcctatt tttagatcac ctttctagat aattaataac
     9601 actatcttca ttttaaagat taaagatatt aatcaaagtg aatttaaaca gatcactcaa
     9661 ggtatcatag caaacatact ccatgctatt tgatcataaa atgtctccct gattccccct
     9721 gaaagtacac attgtcactg ttatcaagtt ctaggacaca aactgtgtac cctgtcacgc
     9781 ctggccacct attctcagta aaccaattca aaagggcaaa ttaaaagtaa aggcgcataa
     9841 cgataccacg atatcaacaa gtttgtacaa aaaagcaggc tggcgccgga accgaagttc
     9901 ctattccgaa gttcctattc tctagaaagt ataggaactt cgaacccttt cccacaccac
     9961 cctccacact tgccccaaac actgccaact atgtaggagg aaggggttgg gactaacaga
    10021 agaacccgtt gtggggaagc tgttgggagg gtcactttat gttcttgccc aaggtcagtt
    10081 gggtggcctg cttctgatga ggtggtccca aggtctgggg tagaaggtga gagggacagg
    10141 ccaccaaggt cagccccccc cccctatccc ataggagcca ggtccctctc ctggacagga
    10201 agactgaagg ggagatgcca gagactcagt gaagcctggg gtaccctatt ggagtccttc
    10261 aaggaaacaa acttggcctc accaggcctc agccttggct cctcctggga actctactgc
    10321 ccttgggatc cccttgtagt tgtgggttac ataggaaggg ggacgggatt ccccttgact
    10381 ggctagccta ctcttttctt cagtcttctc catctcctct cacctgtctc tcgacccttt
    10441 ccctaggata gacttggaaa aagataaggg gagaaaacaa atgcaaacga ggccagaaag
    10501 attttggctg ggcattcctt ccgctagctt ttattgggat cccctagttt gtgataggcc
    10561 ttttagctac atctgccaat ccatctcatt ttcacacaca cacacaccac tttccttctg
    10621 gtcagtgggc acatgtccag cctcaagttt atatcaccac ccccaatgcc caacacttgt
    10681 atggccttgg gcgggtcatc ccccccccca cccccagtat ctgcaacctc aagctagctt
    10741 gggtgcgttg gttgtggata agtagctaga ctccagcaac cagtaacctc tgccctttct
    10801 cctccatgac aaccaggctc ccaggtcccg aaaaccaaag aagaagaacg cagatctcta
    10861 gagtcaccga gttgctttac cggatgaaga gggccgaaac atactgtcca aggcccttgc
    10921 tggcaatcca cccaactgaa gccagacaca aacagaaaat tgtggcaccg gtgaaacaga
    10981 ctttgaattt tgaccttctc aagttggcgg gagacgtcga gtccaacccc ggaccggtgc
    11041 atgtggaccc caagaaaaag cggaaggtgg accctaaaaa gaaacgcaaa gtggatccta
    11101 agaaaaaaag aaaggtgcac gtgtccatgg tgtccaaggg cgaggaactg ttcaccggcg
    11161 tggtgcccat cctggtggaa ctggatggcg acgtgaacgg ccacaagttc agcgtgtccg
    11221 gcgagggcga aggcgacgcc acatacggaa agctgaccct gaagttcatc tgcaccaccg
    11281 gcaagctgcc cgtgccttgg cctaccctcg tgaccacact gacctacggc gtgcagtgct
    11341 tcagcagata ccccgaccat atgaagcagc acgacttctt caagagcgcc atgcccgagg
    11401 gctacgtgca ggaaagaacc atcttcttta aggacgacgg caactacaag accagggccg
    11461 aagtgaagtt cgagggcgac accctcgtga acagaatcga gctgaagggc atcgacttca
    11521 aagaggacgg caacatcctg ggccacaagc tggagtacaa ctacaacagc cacaacgtgt
    11581 acatcatggc cgacaagcag aaaaacggca tcaaagtgaa cttcaagatc cggcacaaca
    11641 tcgaggacgg ctccgtgcag ctggccgacc actaccagca gaacacccct atcggcgacg
    11701 gccctgtgct gctgcctgac aaccactacc tgagcaccca gagcgccctg agcaaggacc
    11761 ccaacgagaa gagggaccac atggtgctgc tggaattcgt gaccgccgct ggcatcaccc
    11821 tgggcatgga cgagctgtac aagcctgcag gcgagggcag aggaagtctt ctaacatgcg
    11881 gtgacgtgga ggagaatccc gggccgtcca atttactgac cgtacaccaa aatttgcctg
    11941 cattaccggt cgatgcaacg agtgatgagg ttcgcaagaa cctgatggac atgttcaggg
    12001 atcgccaggc gttttctgag catacctgga aaatgcttct gtccgtttgc cggtcgtggg
    12061 cggcatggtg caagttgaat aaccggaaat ggtttcccgc agaacctgaa gatgttcgcg
    12121 attatcttct atatcttcag gcgcgcggtc tggcagtaaa aactatccag caacatttgg
    12181 gccagctaaa catgcttcat cgtcggtctg ggctgccacg accaagtgac agcaatgctg
    12241 tttcactggt tatgcggcgg atccgaaaag aaaacgttga tgccggtgaa cgtgcaaaac
    12301 aggctctagc gttcgaacgc actgatttcg accaggttcg ttcactcatg gaaaatagcg
    12361 accgctgcca ggatataagg aatctggcat ttctggggat tgcttataac accctgttac
    12421 gtatagccga aattgccagg atcagggtta aagatatctc acgtactgac ggtgggagaa
    12481 tgttaatcca tattggcaga acgaaaacgc tggttagcac cgcaggtgta gagaaggcac
    12541 ttagcctggg ggtaactaaa ctggtcgagc gatggatttc cgtctctggt gtagctgatg
    12601 atccgaataa ctacctgttt tgccgggtca gaaaaaatgg tgttgccgcg ccatctgcca
    12661 ccagccagct atcaactcgc gccctggaag ggatttttga agcaactcat cgattgattt
    12721 acggcgctaa ggatgactct ggtcagagat acctggcctg gtctggacac agtgcccgtg
    12781 tcggagccgc gcgagatatg gcccgcgctg gagtttcaat accggagatc atgcaagctg
    12841 gtggctggac caatgtaaat attgtcatga actatatccg taacctggat agtgaaacag
    12901 gggcaatggt gcgcctgctg gaagatggcg atctcgagcc atctgctgga gacatgagag
    12961 ctgccaacct ttggccaagc ccgctcatga tcaaacgctc taagaagaac agcctggcct
    13021 tgtccctgac ggccgaccag atggtcagtg ccttgttgga tgctgagccc cccatactct
    13081 attccgagta tgatcctacc agacccttca gtgaagcttc gatgatgggc ttactgacca
    13141 acctggcaga cagggagctg gttcacatga tcaactgggc gaagagggtg ccaggctttg
    13201 tggatttgac cctccatgat caggtccacc ttctagaatg tgcctggcta gagatcctga
    13261 tgattggtct cgtctggcgc tccatggagc acccagtgaa gctactgttt gctcctaact
    13321 tgctcttgga caggaaccag ggaaaatgtg tagagggcat ggtggagatc ttcgacatgc
    13381 tgctggctac atcatctcgg ttccgcatga tgaatctgca gggagaggag tttgtgtgcc
    13441 tcaaatctat tattttgctt aattctggag tgtacacatt tctgtccagc accctgaagt
    13501 ctctggaaga gaaggaccat atccaccgag tcctggacaa gatcacagac actttgatcc
    13561 acctgatggc caaggcaggc ctgaccctgc agcagcagca ccagcggctg gcccagctcc
    13621 tcctcatcct ctcccacatc aggcacatga gtaacaaagg catggagcat ctgtacagca
    13681 tgaagtgcaa gaacgtggtg cccctctatg acctgctgct ggaggcggcg gacgcccacc
    13741 gcctacatgc gcccactagc cgtggagggg catccgtgga ggagacggac caaagccact
    13801 tggccactgc gggctctact tcatcgcatt ccttgcaaaa gtattacatc acgggggagg
    13861 cagagggttt ccctgccaca gcttgatgaa gatctgagct cttaattaat gatcataatc
    13921 agccatatca catctgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
    13981 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
    14041 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
    14101 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat ccgtttaaac
    14161 taactttaaa taattggcat tatttaaagt tacatatggc ggccaattcg ccttctgcag
    14221 gagcgtacag aacccagggc cctggcaccc gtgcagaccc tggcccaccc cacctgggcg
    14281 ctcagtgccc aagagatgtc cacacctagg atgtcccgcg gtgggtgggg ggcccgagag
    14341 acgggcaggc cgggggcagg cctggccatg cggggccgaa ccgggcactg cccagcgtgg
    14401 gcgcgggggc cacggcgcgc gcccccagcc cccgggccca gcaccccaag gcggccaacg
    14461 ccaaaactct ccctcctcct cttcctcaat ctcgctctcg ctcttttttt ttttcgcaaa
    14521 aggaggggag agggggtaaa aaaatgctgc actgtgcggc gaagccggtg agtgagcggc
    14581 gcggggccaa tcagcgtgcg ccgttccgaa agttgccttt tatggctcga gcggccgcgg
    14641 cggcgcccta taaaacccag cggcgcgacg cgccaccacc gccgagaccg cgtccgcccc
    14701 gcgagcacag agcctcgcct ttgccgatcc tctagagtcg agatccgccg ccaccatgat
    14761 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
    14821 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
    14881 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
    14941 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
    15001 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
    15061 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
    15121 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
    15181 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
    15241 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
    15301 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
    15361 cttttctgga ttcatcgact gtggccggct gggtgtggcc gaccgctatc aggacatagc
    15421 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
    15481 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
    15541 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
    15601 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
    15661 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
    15721 cccccggatc taagctctag ataagtaatg atcataatca gccatatcac atctgtagag
    15781 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
    15841 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
    15901 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
    15961 ctcatcaatg tatcttatca tgtctggatc cctaacttta aataatgcca attatttaaa
    16021 gttaggaatt aattcaccca gctttcttgt acaaagtggt tgatatctct atagtcgcag
    16081 taggcggttc taggtcctga aatgagagca aggttcaaaa cagatacaca catctaagca
    16141 gtcctggtca cggcgtgatc tcacctaccc tgagccaatc cttgtctggg cttcagtctc
    16201 atcctgtaag gaagtctaac aagctgttag atctgtgtga aatctttctc caggagacaa
    16261 gtttcccctc tggctggtgc ccttctcttc tcctagcatg agattcatga gaaaacaccc
    16321 atttccttga ggttagttgt ttcaatagtc tgcttgtctg atggagaggt gcacaggcct
    16381 ctttagaaca tcttcactcc tatcgggcca gttacaattt acatgtatgt gtaaatgcca
    16441 ctgagatatt aaccagtctg tttctgtgtt taggttgcgg ctttgaagtc atgcaatagg
    16501 agctacggag tgtgtgtgat tatgggagta gctccgaccg gttcccagct ctcttttgac
    16561 ccattggtac ttctccctgg aagaacccta aaatcttctg ttttgggagg taggtctctc
    16621 agtttcctct ctcttttaga accctaaatc cccctgcatg ctctttggaa ggaaacaagg
    16681 aaggaagacc ggaagaagta gaaggcaagg cagtgtatca aaaatgataa ggtgaggtgg
    16741 gcctggagtc tcttgcatac aagccagaga tggcgcaacg caattaatga taacttcgta
    16801 tagcatacat tatacgaagt tatggtctga gctcgccatc agttcagcag tcaggtatac
    16861 acagaaagac cctcttctaa cttccaaata ggacaagtct atcttcccga aagcagtgtg
    16921 cccctgtaga aacaaacaga gtaccagtcc aacgctgaaa agacatagaa gatgtactcg
    16981 gtgacaagcc atggatggcc tatggaccac tcctccgtag agaggaatat atcaacaagc
    17041 catggatggc ctatggacca ctcctccgta aagaggaata tatcactgat ttggtccctg
    17101 cgttctcatc tttatactca gaccactttt ctattttaaa acaaaacaga tcattacaat
    17161 gtgggtcaca attcatcctc tcttctctaa atacctccag attctattcc atcactggtc
    17221 ataccccata tgcctcccag cctaacatgt ctcggggaac acgtgcagtg ataaatgttc
    17281 tgaggccata ttgtctaatg cagtgacttc tggtcgcatg tggctattgg ctactcagaa
    17341 gatggctagt gggacccgga ggatgatgag tcagttgtgt ttcactcttc tttatttaaa
    17401 ttcgaagaac gacagtggat gtcacagcac atgcctcact gcacgtgcta ggaacacaga
    17461 gggaagtcga tttctgtgag ttcaaggcta ccttgatctg catagtgact aagtttcagg
    17521 gtagccaggg tgacatactg agaccttgcc taaaaagtaa ggaaataaat gcaagcaatc
    17581 aagtatgact agcattgccg tattattaga tagcacggct catattatct tataccctag
    17641 ccttaaccag agtactcaag caagcctgag gattgatgag tggtcattgg tgattgatta
    17701 atgatggatt gattggttga ttgattgatg gttgttaatt taaggtctgg aatcgaaaaa
    17761 cttggctttt ggctgtcaga gttctcgatg ggcactaccc ataaacaagt cattcattct
    17821 cttaatccca gtccagggtg agcagtagct tttcttatag agtgagaact aaatgaaaaa
    17881 tatacacatt aaagcgttta ccagtaaaaa taagccatca gttgaggtat tggttatggt
    17941 agcccacaac ccaagaaaac ctgaggccca agactgccaa agataaccaa agtggcttgg
    18001 ctttgctttg gtggcaatgt gtgtttgttt gttggcttga atctctggga ccctctccat
    18061 tattccttga caggcttttg tgtaagtgca atatgaagtt ggattactgc ccagctttaa
    18121 cagagagaat gtttccactg accagtcttc cactataaaa atttcatctt tcagacactc
    18181 aaaacttatc taactgtgta aaacttccca aactaaccca tgtaaagaga aactgagtgc
    18241 tgagaatagc tatctatcct tgtccatttt ctgttaaggc taagaaaaac acctgtgggt
    18301 agttaatgta taagaaaaag acgtttgttt tgcttttgtt aagatttttt tttattcatt
    18361 tttcttctta tgtgtgagtg ttttgctgca tgtctgtata tgcatcattt gtgtgcttgg
    18421 tgccctcaaa gattacatgg aagagggtct cagagcctct aaatctgaag ttacagatgg
    18481 tcatgagcaa ctatgtgagg gctaggaatt ggtcccaggt ctgctagaag agacactagt
    18541 gctctttagt actaagccac ctctccaacc gaagtcagga tatttttatc agagcaacag
    18601 aaaaaagaat ctgaaattga catcaaatag gaagaggggg gatttgtata gccaagatga
    18661 cctcaagtgt gtggcatagg attcagaccc atgcctgtgg ctgtcgggta cagccttctg
    18721 gttcagcacc aaggtctact ctgcagcagg agcagtaccc actcagcaag agagaagctg
    18781 ggaagtaaac atcaggggaa ggattgcctg ccgggctctc actgggccaa tggctcttcc
    18841 tgtcccaaga caaggttctc tgctgccctc ctctcatgag actgacatcg tccaggttct
    18901 gtagtttctt ttcctgtcag gcactatgct accttagagg cttttccttg cttttaggag
    18961 gaccctagaa acccaagcaa cggctgcaaa agtgagagtg tttctctcca ggtgtctggg
    19021 tttaccagtt ttgtgcctgg ctgccctatt tccagatttc aattttgtac ctttattttc
    19081 accagctacc aatgtcaaaa tctacagttt attcccaaac cttcttgctg tgctgtgtgt
    19141 gtctcctggt gctattgacg acaccaagtg tgtaatggcg tgagagcgtg ctgtagaggt
    19201 acaccagata ctcacccttg gttctcagtt accagtgaat aaaactaata aacttagtcc
    19261 ctttaaaaga attaataaaa ttcccaaaca ataaaaatgt tatggaaaaa aaggaattat
    19321 ttcattatag atgaaagaag ctataataga catagtcttg tttttccagt tttatgctaa
    19381 ccggtttcta ggctagtgaa aaacctgcat accaactggt tgtgcactca tcagttattg
    19441 tattgtatct gtgatatgga tgcttaatgt ccctcaggta tgtaagttaa aggtccccag
    19501 gatggtgcta ctgatatgtt ctgtggtcct ttcggaggta agtcttctgg gaaggcctta
    19561 ggtcactgga tgtacgattt cacatgattc tgtgctggtg ttttttcctc tgtaaaagaa
    19621 aagaacagaa aaaatagact caatgcaatc atgctcgaag tgcatcagcc catggctctc
    19681 cattgttctc aggacactaa ctacttccta taatgttcac cccccagctg atttgatctg
    19741 agttaatctc agaatcttga atgaagtgag actggcaacc cagatcaatg agctagcgag
    19801 ggtgcaggaa atggtcagtg ccctgaagac aaagaagagc tttagctaca tgggttatag
    19861 ccatgagggt gtcaggtagg agcgctctcc tagggccctg gtaatcataa ttctgtccat
    19921 gactggattc ttctatggaa caaactgctt tgacacttgg ataaagaatc aatggggtaa
    19981 tgaatgactt actgcaccca taaacacatt gataattgta taaattatta aatttattat
    20041 agattattaa ttataatatt atcactggaa tcttcagtca gatttgatat gtttcacatc
    20101 ctattaattt tgtcctttcc ttctacaggg tataaaacca gagatgacat ccctaaacta
    20161 gtaactgact acgttcaaaa gaaaattaat atagatcctc ttataaccca tagactgcct
    20221 ttcccaaaaa tcaatgaagg attcagattg ttgcaagagg gaaaatggta agtattcatg
    20281 tgaatggggt gagtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtacaaga cattcctggg
    20341 tgtggtgaca tacatgtgac ttccagtcaa aatcttaaga aaagccctct atgctcccat
    20401 ataactttcc attaccctca tgatgacttg tgacagtaaa tggcaattaa cctgcctgat
    20461 gagtttttga atatcacatt tagatctcta acaccagcct caactgcaat atgtcaagtg
    20521 caggcattgg tcaggaataa gctttggctt aaaagaacaa ataagtagga aggtaaacgg
    20581 ataaaagcaa actttccacc catga
//