LOCUS       27268#pL1L2GT1_LF2A_nEGFPOT2A_CreERT2_pAbActNeopA#L3L4_pD223_DTA_T_spec        20759 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2GT1_LF2A_nEGFPOT2A_CreERT2_pAbActNeopA
COMMENT     design_id: 27268
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000102710
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            7923..8025
                     /db_xref="ENSEMBL:ENSMUSE00000662362"
                     /rank="2"
                     /note="ENSMUSE00000662362"
     misc_feature    4275..8861
                     /note="5 arm"
     misc_feature    8889..8913
                     /note="B1 Gateway"
     misc_feature    9880..9897
                     /note="FCHK QC critical region"
     misc_feature    9864..9879
                     /note="FCHK QC prefix region"
     misc_feature    9898..9903
                     /note="FCHK QC suffix region"
     misc_feature    10026..10077
                     /note="F2A"
     misc_feature    9903..10025
                     /note="1Dleader"
     primer          9749..9770
                     /vntifkey="27"
                     /dnas_title="seq primer FCHK2"
                     /label=seq primer FCHK2
                     /note="seq primer FCHK2"
     exon            9849..9902
                     /vntifkey="61"
                     /dnas_title="mouse En2 exon"
                     /label=mouse En2 exon
                     /note="mouse En2 exon"
     misc_feature    10949..10949
                     /note=" A to G to kill RsRII site"
     misc_feature    10899..10952
                     /note="self-cleaving' 2A peptide"
     misc_feature    10953..11978
                     /note="Cre"
     misc_feature    10083..10172
                     /note="3X NLS"
     misc_feature    10173..10889
                     /note="EGFP"
     primer          complement(9042..9063)
                     /vntifkey="27"
                     /dnas_title="LRPCR LAR3_EN2"
                     /label=LRPCR LAR3_EN2
                     /note="LRPCR LAR3_EN2"
     intron          8974..9848
                     /vntifkey="21"
                     /dnas_title="mouse En2 intron"
                     /label=mouse En2 intron
                     /note="mouse En2 intron"
     primer          complement(8938..8961)
                     /vntifkey="27"
                     /dnas_title="FRT3_oligo"
                     /label=FRT3_oligo
                     /note="FRT3"
     misc_feature    8926..8973
                     /vntifkey="21"
                     /dnas_title="Frt"
                     /label=Frt
                     /note="Frt"
     misc_feature    13239..13246
                     /vntifkey="21"
                     /dnas_title="polylinker"
                     /label=polylinker
     misc_recomb     13207..13238
                     /note="Rox(inv)"
     misc_feature    13199..13206
                     /vntifkey="21"
                     /dnas_title="RE sites"
                     /label=RE sites
     primer          13139..13157
                     /vntifkey="27"
                     /dnas_title="seq primer NF"
                     /label=seq primer NF
     primer          13013..13036
                     /vntifkey="27"
                     /dnas_title="LRPCR RAF5"
                     /label=LRPCR RAF5
     misc_feature    12956..13198
                     /vntifkey="21"
                     /dnas_title="SV40 pA"
                     /label=SV40 pA
     misc_feature    11988..12932
                     /note="ERT2"
     misc_feature    14446..14446
                     /note="G to C conservative mutation to kill RsrII site"
     misc_feature    14794..15037
                     /note="SV40pA"
     misc_feature    14852..14875
                     /note="RAF5"
     misc_feature    14601..14620
                     /note="neoBstB1_5"
     misc_feature    14308..14326
                     /note="neoSphI_5"
     misc_feature    13971..13990
                     /note="neoPstI_5"
     misc_feature    13802..14787
                     /note="neomycin resistance "
     misc_feature    13652..13671
                     /note="betact5"
     promoter        13256..13776
                     /note="human beta actin promoter"
     insert          12953..15032
                     /source="pL1L2_GTIRES_BetactP FLFL MapVerified"
     misc_feature    15033..15037
                     /note="for bActNeo"
     misc_recomb     15039..15070
                     /note="Rox"
     misc_feature    15083..15103
                     /note="B2 Gateway"
     misc_feature    8862..15133
                     /note="Synthetic Cassette"
     exon            15549..15570
                     /db_xref="ENSEMBL:ENSMUSE00000662361"
                     /type="targeted"
                     /rank="3"
                     /note="target exon 1 ENSMUSE00000662361"
     misc_feature    15134..15744
                     /note="Critical Region"
     misc_feature    15768..15801
                     /note="Downstream LoxP"
     misc_feature    15745..15824
                     /note="synthetic loxP region"
     exon            19441..19679
                     /db_xref="ENSEMBL:ENSMUSE00000165654"
                     /rank="5"
                     /note="ENSMUSE00000165654"
     exon            18316..18367
                     /db_xref="ENSEMBL:ENSMUSE00000165678"
                     /rank="4"
                     /note="ENSMUSE00000165678"
     misc_feature    15825..20759
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggatacgta gcatggagtt catctgaatg aagcatataa cacagaagat
     4321 tttagtgcat ctatagatta tgcaaccttc cctgtagttc agaatttgaa tattttcctc
     4381 atccttagta gaacctgtct tcattaccag ctactctttc ttgaactccc ttctacagct
     4441 gctccaggct tagaccatca cttactgtct ctattggttg tccctactgg acatttcata
     4501 cacacaaatg gaaacaggta atatgtgggg gtagaggttg ccactgtctt cattcactga
     4561 gtatgatttg taggtttatc cttggcagtt tgaatcagat gttcaattac acattccata
     4621 actagtagaa catttaggca tagatactta tgagagtgag ttcaacacac ttcatctaca
     4681 cactgaacac tataccttat tgctttagga tttccattgc tgtgaagaca caccatgacc
     4741 aaggcaactc ttatataaag gttaccattt cattggggct ggcttacaga ggtttagtcc
     4801 attatcatca tggtgggaag catggcagtg accagacaga cttgatgctg gaggagccat
     4861 ctatgggggg ctattctatt caaatgacca cacacagttt taatttttgt ctctttgaaa
     4921 taagtaagtt aaactccata ttgagactta atggcctata ctgaggtatt ttgttagttc
     4981 ttatgtttat gcttggttct ttttcataat cctttctagt tactctttac ttctgaagtc
     5041 cactctgtat tagttcactc tgcatcctcc cttcattctc aatgtcagga tactgacaaa
     5101 ctgacttttt tgacccaggt taaaagttat ttttcctcag cactttgaag atgtttatct
     5161 gctgttttcc agtattagtt actgataaaa aatatatctg tcagttatta tcacttattt
     5221 catagattta agacaggttg tagttattct tgatgctcta cactttccat ataaattgtc
     5281 tattgactga tttttgttta tgctgatcaa cccttggaat ggctaacagc tgttgttgct
     5341 gtaacaaaat accttccaca aacaagagta aaggttaatt ttgtctcatg gctaactcta
     5401 cttcttgagg ccctaggcaa ggctgaataa catggtggtg ggtgtgagtg aaggatgaga
     5461 cttctcaccc tgtcatgact aagaaacaga gagagagaca tgaaggagcc aggacatgtt
     5521 ctcagtgact caccttctac agttccacca tcttccaatc gaatattcca attctgggtc
     5581 aggaaatgga ttactcaatt tatgaggaca gaatcctcac tatctaatca tttcctcaaa
     5641 gtgtggggag cgggtgtggc ggcagtccca aaggcgccag agactgcagc taagtcgtat
     5701 gacttgcacc tgacttcctc atataagcca caaacatctt gagtgctgcg caggtgtacc
     5761 aggatacagg tgaatccaat ttggtggaga tttgcccctg ctgccctgat tagctgaagc
     5821 tgcgtgcctg gtgaggtggc gtggcctgcg gtgcgtggat gagagagagt ataaaaggag
     5881 tgagaggccc agggttcgga ggagatataa aaacaaggga gatataaaaa caagggagat
     5941 ataaacaggg gagatataaa aacaagggag atataaaaac aagggagata tatggagaaa
     6001 gaagaaacag gactgaataa atgtgtgcag aaggatcctg tagcagcgtc gttcttcctg
     6061 gccagttgag cgcgcgcaac atcaaagccc cataactgtg catcactcct ttgcacaggg
     6121 accaggcctt caaggcatgc atgacataac atagaatcta gtgttaatta tatcactgct
     6181 tttagaaaat tgctagcaat gatctaatta ctgtatctgt catttccatc ctctgtgatc
     6241 ccaattaggt aaagctgaga gatttttact agtccatctt caactctttt atatgcatag
     6301 tatgcagatg taatcatata tatacacaca gcatgtgtat atgtatatgt aacatatacc
     6361 actgtctcct tatataacct ttatagatct gcatttcatc atgagctata agtccttggg
     6421 caacgattgg cactaagagt tcaggagcct tctagcagtg ctgggggtct agaatagtca
     6481 atagctccac ctagaaagga gctctttaat ctgctttttt caggagctaa actctcaagt
     6541 ctcccatatg tatctagaac ctatagtgaa aataccaact ggggtgctct tttctcttcc
     6601 acttaagctt cattttattt tgattgttta tttttattgt cttgagcctg cttgaatttt
     6661 taaaaacacc actcatggtt gatatgtatt caaagtagag aaaactcata aagagagctg
     6721 tgattaaaac taatgctaac ttcttgtttt attcagggcc ttctctgctt catctagact
     6781 caccttcccc tggggctcta cttacgaaag gccactgaca ctacctgaaa ggtcttgggt
     6841 gttattattg taccatgtat actaaagaat agcatcaact gttttgttct tttagtacta
     6901 taatgatgat ttgttaaaaa taggtaaaac acatataaac agaaagagca cataaattta
     6961 taaacaatca ttgtctactc accaactaaa gaaaatcatc ttctttcttt taactagatg
     7021 gtcttttatt gaaatatttc ctcttgttgc ttcttagcta gctggacact ccactgcacc
     7081 actgttgatg tcatctatga catcatgagg atggcagcca ttcatagttc aacccacaga
     7141 ctatgtagta cccaggttct taatatttcc agaaagctct ctggctaaag accactgtca
     7201 catatgctag gcaatgttga taatctcatt aaaagtaaca tttctattgt gcttaatgtt
     7261 ctcttgtttt tttttctgtc tcttggtggc tctgagaggg ttttggttgt catgaagagg
     7321 cagagggcac cacctaaatc tggttctgtc tgttctagag tgtcagtgtc atgctaatga
     7381 catgtcagtg tccagttact gattgctttg gtaataccat gaccaacatt tttttttttt
     7441 gaggcagacc aaaggaacca atcttgatga ccaaggcaga tgtagcagtg gtccctgcca
     7501 atggtgaacc tcacatgcac aattttcata tccttaggga caaagttggg tggcacggtg
     7561 taggctgttg gtatctgatg aacctgaatt tggaagactg aagaaagctg tactgtagct
     7621 tcctctgagc caaaagctga aaagcaagtc aaccatttca actgggctac cttttgaaaa
     7681 gttggcaaaa gtcatttgca taacaattaa tgaacacatg actctaatcc attctcagtt
     7741 tctttcctaa tatttagtct ctttcattgt tatgttatta aattataaga tctggggggt
     7801 tttgtttgtt tgtttgaggc aaattataga agcttgtttc ttgagccaag tcccttgctt
     7861 atttagctac ttttgttaaa gagaaaatgt ataataattg cagcaactgt tttattttat
     7921 agggttgttg agtgaagatt gcagacacca tgtctgactc agtgattctt cgcagtgtga
     7981 agaaatttgg agaggagaat catgctttcg aatcagatgg tttccgtaag cgagtttcct
     8041 tgatccttgt gtaaagaaaa ctaaagacaa gagtgagaca cgcctagtga ggtttcaaaa
     8101 agcagagaaa aatcagctac aacatggggg gctagggcta ggcatgtgcc tgacatacaa
     8161 ccaaactgga cgggctctct agaattcatg tcacatctga aagctagctc tgtgttaaaa
     8221 tgcacaacat gagtgagtgc taacatctcc catgagcact tccttctcag tagagtgatc
     8281 actgtataac tttagcccca tagcattgcc gatcaccatg gcaacgataa ttgttaatgg
     8341 tttctgctcc ttaaacaggc cctaccaaca ctctcctggc agaaatgagg ggccttgaca
     8401 cattagtcct tggcatgttc taagctgcta ttcatccatt cattcattca ttcacgaatc
     8461 aaaacaatcc atgcaatcat gcttctaatc cagttttcca attttcaagc cctttatcca
     8521 cactataatg tctctttagt ctttttctgc aactgtatta aatgtcattg gaaccaggaa
     8581 atggtggcca tggtaacaaa tattccttag agttcttttc tctgtggctt ccttatgtcc
     8641 ctgtagacac tcacttccat ttcctactga catctgctct acatgggctg acaaaaataa
     8701 tatgtagctc tgtcttatgg attctggtgg cgatgaggta ttaaagacat gttacagtca
     8761 atttgtgatc tgttaagtag ataaattttg acaaatagaa aatgtttcta aggaaccaaa
     8821 caatagatta cttttcatct taatagataa taccaaatga caaggcgcat aacgatacca
     8881 cgatatcaac aagtttgtac aaaaaagcag gctggcgccg gaaccgaagt tcctattccg
     8941 aagttcctat tctctagaaa gtataggaac ttcgaaccct ttcccacacc accctccaca
     9001 cttgccccaa acactgccaa ctatgtagga ggaaggggtt gggactaaca gaagaacccg
     9061 ttgtggggaa gctgttggga gggtcacttt atgttcttgc ccaaggtcag ttgggtggcc
     9121 tgcttctgat gaggtggtcc caaggtctgg ggtagaaggt gagagggaca ggccaccaag
     9181 gtcagccccc cccccctatc ccataggagc caggtccctc tcctggacag gaagactgaa
     9241 ggggagatgc cagagactca gtgaagcctg gggtacccta ttggagtcct tcaaggaaac
     9301 aaacttggcc tcaccaggcc tcagccttgg ctcctcctgg gaactctact gcccttggga
     9361 tccccttgta gttgtgggtt acataggaag ggggacggga ttccccttga ctggctagcc
     9421 tactcttttc ttcagtcttc tccatctcct ctcacctgtc tctcgaccct ttccctagga
     9481 tagacttgga aaaagataag gggagaaaac aaatgcaaac gaggccagaa agattttggc
     9541 tgggcattcc ttccgctagc ttttattggg atcccctagt ttgtgatagg ccttttagct
     9601 acatctgcca atccatctca ttttcacaca cacacacacc actttccttc tggtcagtgg
     9661 gcacatgtcc agcctcaagt ttatatcacc acccccaatg cccaacactt gtatggcctt
     9721 gggcgggtca tccccccccc cacccccagt atctgcaacc tcaagctagc ttgggtgcgt
     9781 tggttgtgga taagtagcta gactccagca accagtaacc tctgcccttt ctcctccatg
     9841 acaaccaggc tcccaggtcc cgaaaaccaa agaagaagaa cgcagatcgc agatcgcaga
     9901 tctctagagt caccgagttg ctttaccgga tgaagagggc cgaaacatac tgtccaaggc
     9961 ccttgctggc aatccaccca actgaagcca gacacaaaca gaaaattgtg gcaccggtga
    10021 aacagacttt gaattttgac cttctcaagt tggcgggaga cgtcgagtcc aaccccggac
    10081 cggtgcatgt ggaccccaag aaaaagcgga aggtggaccc taaaaagaaa cgcaaagtgg
    10141 atcctaagaa aaaaagaaag gtgcacgtgt ccatggtgtc caagggcgag gaactgttca
    10201 ccggcgtggt gcccatcctg gtggaactgg atggcgacgt gaacggccac aagttcagcg
    10261 tgtccggcga gggcgaaggc gacgccacat acggaaagct gaccctgaag ttcatctgca
    10321 ccaccggcaa gctgcccgtg ccttggccta ccctcgtgac cacactgacc tacggcgtgc
    10381 agtgcttcag cagatacccc gaccatatga agcagcacga cttcttcaag agcgccatgc
    10441 ccgagggcta cgtgcaggaa agaaccatct tctttaagga cgacggcaac tacaagacca
    10501 gggccgaagt gaagttcgag ggcgacaccc tcgtgaacag aatcgagctg aagggcatcg
    10561 acttcaaaga ggacggcaac atcctgggcc acaagctgga gtacaactac aacagccaca
    10621 acgtgtacat catggccgac aagcagaaaa acggcatcaa agtgaacttc aagatccggc
    10681 acaacatcga ggacggctcc gtgcagctgg ccgaccacta ccagcagaac acccctatcg
    10741 gcgacggccc tgtgctgctg cctgacaacc actacctgag cacccagagc gccctgagca
    10801 aggaccccaa cgagaagagg gaccacatgg tgctgctgga attcgtgacc gccgctggca
    10861 tcaccctggg catggacgag ctgtacaagc ctgcaggcga gggcagagga agtcttctaa
    10921 catgcggtga cgtggaggag aatcccgggc cgtccaattt actgaccgta caccaaaatt
    10981 tgcctgcatt accggtcgat gcaacgagtg atgaggttcg caagaacctg atggacatgt
    11041 tcagggatcg ccaggcgttt tctgagcata cctggaaaat gcttctgtcc gtttgccggt
    11101 cgtgggcggc atggtgcaag ttgaataacc ggaaatggtt tcccgcagaa cctgaagatg
    11161 ttcgcgatta tcttctatat cttcaggcgc gcggtctggc agtaaaaact atccagcaac
    11221 atttgggcca gctaaacatg cttcatcgtc ggtctgggct gccacgacca agtgacagca
    11281 atgctgtttc actggttatg cggcggatcc gaaaagaaaa cgttgatgcc ggtgaacgtg
    11341 caaaacaggc tctagcgttc gaacgcactg atttcgacca ggttcgttca ctcatggaaa
    11401 atagcgaccg ctgccaggat ataaggaatc tggcatttct ggggattgct tataacaccc
    11461 tgttacgtat agccgaaatt gccaggatca gggttaaaga tatctcacgt actgacggtg
    11521 ggagaatgtt aatccatatt ggcagaacga aaacgctggt tagcaccgca ggtgtagaga
    11581 aggcacttag cctgggggta actaaactgg tcgagcgatg gatttccgtc tctggtgtag
    11641 ctgatgatcc gaataactac ctgttttgcc gggtcagaaa aaatggtgtt gccgcgccat
    11701 ctgccaccag ccagctatca actcgcgccc tggaagggat ttttgaagca actcatcgat
    11761 tgatttacgg cgctaaggat gactctggtc agagatacct ggcctggtct ggacacagtg
    11821 cccgtgtcgg agccgcgcga gatatggccc gcgctggagt ttcaataccg gagatcatgc
    11881 aagctggtgg ctggaccaat gtaaatattg tcatgaacta tatccgtaac ctggatagtg
    11941 aaacaggggc aatggtgcgc ctgctggaag atggcgatct cgagccatct gctggagaca
    12001 tgagagctgc caacctttgg ccaagcccgc tcatgatcaa acgctctaag aagaacagcc
    12061 tggccttgtc cctgacggcc gaccagatgg tcagtgcctt gttggatgct gagcccccca
    12121 tactctattc cgagtatgat cctaccagac ccttcagtga agcttcgatg atgggcttac
    12181 tgaccaacct ggcagacagg gagctggttc acatgatcaa ctgggcgaag agggtgccag
    12241 gctttgtgga tttgaccctc catgatcagg tccaccttct agaatgtgcc tggctagaga
    12301 tcctgatgat tggtctcgtc tggcgctcca tggagcaccc agtgaagcta ctgtttgctc
    12361 ctaacttgct cttggacagg aaccagggaa aatgtgtaga gggcatggtg gagatcttcg
    12421 acatgctgct ggctacatca tctcggttcc gcatgatgaa tctgcaggga gaggagtttg
    12481 tgtgcctcaa atctattatt ttgcttaatt ctggagtgta cacatttctg tccagcaccc
    12541 tgaagtctct ggaagagaag gaccatatcc accgagtcct ggacaagatc acagacactt
    12601 tgatccacct gatggccaag gcaggcctga ccctgcagca gcagcaccag cggctggccc
    12661 agctcctcct catcctctcc cacatcaggc acatgagtaa caaaggcatg gagcatctgt
    12721 acagcatgaa gtgcaagaac gtggtgcccc tctatgacct gctgctggag gcggcggacg
    12781 cccaccgcct acatgcgccc actagccgtg gaggggcatc cgtggaggag acggaccaaa
    12841 gccacttggc cactgcgggc tctacttcat cgcattcctt gcaaaagtat tacatcacgg
    12901 gggaggcaga gggtttccct gccacagctt gatgaagatc tgagctctta attaatgatc
    12961 ataatcagcc atatcacatc tgtagaggtt ttacttgctt taaaaaacct cccacacctc
    13021 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
    13081 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
    13141 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatccgt
    13201 ttaaactaac tttaaataat tggcattatt taaagttaca tatggcggcc aattcgcctt
    13261 ctgcaggagc gtacagaacc cagggccctg gcacccgtgc agaccctggc ccaccccacc
    13321 tgggcgctca gtgcccaaga gatgtccaca cctaggatgt cccgcggtgg gtggggggcc
    13381 cgagagacgg gcaggccggg ggcaggcctg gccatgcggg gccgaaccgg gcactgccca
    13441 gcgtgggcgc gggggccacg gcgcgcgccc ccagcccccg ggcccagcac cccaaggcgg
    13501 ccaacgccaa aactctccct cctcctcttc ctcaatctcg ctctcgctct tttttttttt
    13561 cgcaaaagga ggggagaggg ggtaaaaaaa tgctgcactg tgcggcgaag ccggtgagtg
    13621 agcggcgcgg ggccaatcag cgtgcgccgt tccgaaagtt gccttttatg gctcgagcgg
    13681 ccgcggcggc gccctataaa acccagcggc gcgacgcgcc accaccgccg agaccgcgtc
    13741 cgccccgcga gcacagagcc tcgcctttgc cgatcctcta gagtcgagat ccgccgccac
    13801 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
    13861 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
    13921 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
    13981 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
    14041 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
    14101 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
    14161 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
    14221 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
    14281 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
    14341 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
    14401 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggccgacc gctatcagga
    14461 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
    14521 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
    14581 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
    14641 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
    14701 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
    14761 gcccaccccc cggatctaag ctctagataa gtaatgatca taatcagcca tatcacatct
    14821 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
    14881 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
    14941 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
    15001 tccaaactca tcaatgtatc ttatcatgtc tggatcccta actttaaata atgccaatta
    15061 tttaaagtta ggaattaatt cacccagctt tcttgtacaa agtggttgat atctctatag
    15121 tcgcagtagg cggtagaaat ggtgatttcc atcaagccca gtagctcaca tctgtaattc
    15181 tagtccatgg gagactgaag caggaaaatt accacaagtc tgaatttgag gctactcttg
    15241 tctatgtagc tcccaaacca gcttacatta cagagtgaga gaccctgtat caaaaaggga
    15301 aagacttcct tttgatattc agaaaataca aatattttat aatctggaaa aaatatccaa
    15361 acatatagtc aaagcagcag gaaatgggga gagaaggagg gatgataaag agatagaaag
    15421 agacagttat tagtttataa tatttcatga ataaaagtgc ttcctagaaa tgcttttggt
    15481 tgactaatga ttatgttgcg tttggacaca attattaacc tttttatacg ttgtcttcat
    15541 cctgacagat aacaatgata agaaatcaag gtgagtgacc gtttcaacac cacagcatct
    15601 tctctagaag gatttcagaa tatttctata aatataggac agagtatttt catctacgtt
    15661 actatgtata gaaagtatag aacacctttt cttttttctt tttccaagac agggtctctg
    15721 tgtggctctg gctgtactgg aactgagatg gcgcaacgca attaatgata acttcgtata
    15781 gcatacatta tacgaagtta tggtctgagc tcgccatcag ttcaccttat aggatagccc
    15841 ttcctgatta tgcccagttt cctcccttgc atcccagtct accacagagc ttctagcctg
    15901 aacaaacagg aaagagctgg acaatagtgt gattttatga gttccaggag gtcctccaac
    15961 tccactcctg ccccctcctc caactctaca ccttctgctc actcctccaa attcccccat
    16021 ctcccctatt tcccaacccc tggagactaa taaaaaccta aagttgaatt ctctgagctt
    16081 tctcttcagc actcgtttga gttcacgtgt aaggaagtgt tgaggcagga ttggatgggg
    16141 ctgcattaga acagtggcac cgtgtgagta cctgacatgg aagatggatg gcagaaggtt
    16201 gatttagaaa gtttaaagta tttcaggaca gaaggcagca gtagtactgg taagattagc
    16261 actttgctag aagaaatctg gtgaccctgg tcctccaaga gtcccattgc gagtgcagaa
    16321 aagaaacatc tattataaat ccttcactgc aagaagggca ttttattttt ttcatttttt
    16381 aaatatataa tgtattttga tcatgttttc tgcctccgat tcctaccatg ttctccccca
    16441 tttccctcct acccacccta ccttagttct ctctcaatct caaaaaaaaa accttcaaaa
    16501 aaaatgaaaa taacaaacaa aaacccaaca agaccaagaa tatcaaaaca aaattaaagg
    16561 aatcaaaaag cccacaacag ccgggaggtg gtggcacagg cctttaatcc tagcacttgg
    16621 gaggcagagg caggcggatt tttgagttcg aggccagcct gatctacaaa gtgagtggca
    16681 ggacagccag ggctacacag agaaaccctg tctcaaaaaa tcaagaaaca aaaacaacaa
    16741 caacaacaaa agcccacaac aagcaaacaa ttatggaatt aattttgtgt cagtcagtcc
    16801 tgggcatggg gcctctttgc tctgtactgt gattgataag cccagtgaca ttctgttgga
    16861 gaaaatggat tatccctttg ccagcaggca tcgattgcaa gaggttaaag cccagagcct
    16921 gtgcccactt cttcctctca gtgctgggac cccatctggt ttgaacctgt gcaggtttca
    16981 ggtgtgttga cacagtctct gtgaattccc gtgtgcaccc gtcctcttgt actctctggg
    17041 gtccttcatc ctctctggtt cttacatctt tttgcattat aaatctctga gccccaagga
    17101 gaggggtttg atgagtgctc caaagtctct tcctctttgc aggttttcag ttgtgaatct
    17161 ctgtgttaat tcccagctac tccaagaaga agcttctctg agcagggcca taggatgctc
    17221 tgacccaggg ctttagcagt aggccattag gagtcattac actgctatgt ttctttagca
    17281 gaataatagt ggtacatccc tctaggctca tgaggaaagt atatttatgg gcagatagaa
    17341 taaacattat aaatttagaa aacataatac ccggtagcta ctgagaatgc ataactattt
    17401 tttttcctaa gaaagagact gaattggaga tgaagctctg agaaggaatg tgtgtgtcag
    17461 ggcagatgtc attgaaatct taacgatctt ggctagagaa ggtcctgaca aggagactta
    17521 ggagacttgg agtgagaatc tgaagttgga gtaactgtcc gcatgagggt ctaagaaagg
    17581 acattttgag ctggagcaca tttggcaaag ataaatggat gggtgattgt tttcatgaca
    17641 actagaatat atacactatt actatacatt ataattgttc tatatatatt ctatgtatcc
    17701 atatctctat ctgtatgtat atatagaaca tatattaata tatatagaac atgtaaaata
    17761 tatatagaat atatggtata gtgtatatat atatatttag aataaatata caaatatata
    17821 cacacatata tacaaattat acaatcaata aatatacaaa aatatatata aatattttat
    17881 attgtatctc ctgctgagtc catttaatgt tgcctgtaca catggcctaa ggctgactgt
    17941 tggattatgg gcaatctact aagaatctta ctcctgaatt aaacagagcc cagcccctgc
    18001 tttcagctgt tattgattac ctgtaactct tcaactatgt gtccgacctt gtggactccc
    18061 ctactccccc ccccccaccc cctgtcctca tgctgtaatg tagattggta tggccctata
    18121 caagtcttgt ccaggcacct acagctgttg ggaattcatc agtgcaaaat acattaaaat
    18181 actgggtttg gccagaattc ccagccagag gggaaatgca tacttattaa agaataattc
    18241 agaaaagcac catgtaggga gaatttctca ccagttttgg gaacatgacc tttaaatgcc
    18301 tttccttctc ttcaggttac aagataagaa gaaaggggaa ggcgctcgag ttggcttctt
    18361 tgaactggta aataatgcac tgtgctgctc cccttggcaa ctttgcaact gtgagttatt
    18421 cccacagtag aggaagagcc aactccttag tcatactggc aggacttaac tctagggatc
    18481 agtcttgtag tcatgagctt taccgaacat atgaatactg gttaaaatcc aatgcttagt
    18541 aaacccactg gctaatatca gtggaataga atccatcgac acacaattat aatcttaata
    18601 aggaaagcct ttacagtaac tcccagggca atagatcaca tatatacatt gttatgagaa
    18661 gtctttagaa attccatagc ttatatatct accacaaatt tgtataggat gcaggcattt
    18721 cctctttctt tctttctttc tttctttctt tctttctttc tttctttctt tctttctttc
    18781 tttctttctt tctttccttc cttcctccct ccctccctcc ctccctccct ccctctctcc
    18841 ctccctccct ccctccctct ctctctctct ctctctctct ctctttattt ctttctctct
    18901 ttctccaaaa tagtgttttt ctgtagcctt gcctgccctg gaatttgctc tgtagactag
    18961 gctggcctcc aactcagaga tctgcctgcc tctggctccg gagtgctggg attaaagggg
    19021 tgtgccacca ccgtttagga tggagcattt ttaagcaaca atttgcctgt tgtccggatg
    19081 ggttctcaga gctgagaggc aacttcctgc gctgttaaat gttcactcag tatgtctacc
    19141 tggtaatggc atctgtgatg aagacttgga aggtagtaaa gtcagctcct ggtctgagga
    19201 gaagaggctc aaagatttgg gcgtaatata aggatctgat gttttagaca attgatagct
    19261 agtactttta cataattagt gtgaagacaa ctgctcagag atgaccccca caagcacctg
    19321 gtgttgaatg ttgtttaaca gaatagaggt gaaattaaat agccctcctg cccatccccc
    19381 caccccatct cccatgtgct ttttacatat agataatctc tgaacccttt gtttttcaag
    19441 tttcgatttt cttcatcaaa agacaactgg ctgatgttta tgggaagtgt gtgcgcattg
    19501 ctccatggaa tggcccagcc aggcatgatt attgtgtttg gtatactgac agatattttt
    19561 gttgaatatg acattgaaag acaagaactc agtattccag aaaaagtgtg tatgaataac
    19621 accattgtat ggatcaacag ctccttcaac cagaacatga caaacggaac aagctgtggg
    19681 taagctactt ttcttttttg ttacacctta aattataatt caaaacatcc aaagacaata
    19741 ccgactgagt tggaagtggc atttagtaca gaagaataga aaaacaaact tacccatatt
    19801 gataaaattc tttaactttg tagctatttc catccttcca ggtttcaaag catcaacaga
    19861 ccttgctaat caaataccta catcaacaat tgactacttg gctcactaaa agtccacaca
    19921 tgacaatatc catacttacg accactaaga agataattga atttcacttg agtctcctgg
    19981 acaaaagctc ctttctgctc accacccctg cctttttttt tcttttttct ttttacccct
    20041 tggctttgtt ttctgtccat gtgtttcatg agcatgctat attagaggaa tatgaatcaa
    20101 tagttctagc gaatagttag ttaagttttc atcaagaggg ctgcacctat gacaaagaag
    20161 gtagccctga agaactaggc agaacattat ggaaatcaga caaggtcggt cactgtgcca
    20221 gagttgtctc tgtgacacgc ccaacagccc actttgtaat cactgagtct gtagtacatg
    20281 tgggctgact cactgttgtg gctctgactt tgtacctgcc taatgctgct ttggatacag
    20341 gaccaagttc attggtatgt atgagtccct gtgctcgggt tcatgactca aaggcagagc
    20401 atatcctgcc tagaaagaca cttggatggc tttctcaaat acatctttat ttttcatatt
    20461 aattactgaa atgtggacag ggttggttta aactgggtct ttctaaatca ttaaaatttt
    20521 tccataatat catctacttt gaggattttt cactgcttca atcaaaaaaa tttgaatgtc
    20581 aactaagtaa catgaatgtg tagaagcatc agggaaggac acactgtcct atgagtcaca
    20641 tccttatttt gtagaacttt ggttagtcac tcagactgtc attcactcaa tggtctttta
    20701 taaaatatta gttacaaact agtgagtgct gggtgatgag aaaacataaa attctatgt
//