LOCUS       88436#pL1L2GT1_LF2A_nEGFPOT2A_CreERT2_pAbActNeopA#L3L4_pD223_DTA_T_spec        20955 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2GT1_LF2A_nEGFPOT2A_CreERT2_pAbActNeopA
COMMENT     design_id: 88436
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000005352
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     misc_feature    4275..10090
                     /note="5 arm"
     misc_feature    10118..10142
                     /note="B1 Gateway"
     misc_feature    11109..11126
                     /note="FCHK QC critical region"
     misc_feature    11093..11108
                     /note="FCHK QC prefix region"
     misc_feature    11127..11132
                     /note="FCHK QC suffix region"
     misc_feature    11255..11306
                     /note="F2A"
     misc_feature    11132..11254
                     /note="1Dleader"
     primer          10978..10999
                     /vntifkey="27"
                     /dnas_title="seq primer FCHK2"
                     /label=seq primer FCHK2
                     /note="seq primer FCHK2"
     exon            11078..11131
                     /vntifkey="61"
                     /dnas_title="mouse En2 exon"
                     /label=mouse En2 exon
                     /note="mouse En2 exon"
     misc_feature    12178..12178
                     /note=" A to G to kill RsRII site"
     misc_feature    12128..12181
                     /note="self-cleaving' 2A peptide"
     misc_feature    12182..13207
                     /note="Cre"
     misc_feature    11312..11401
                     /note="3X NLS"
     misc_feature    11402..12118
                     /note="EGFP"
     primer          complement(10271..10292)
                     /vntifkey="27"
                     /dnas_title="LRPCR LAR3_EN2"
                     /label=LRPCR LAR3_EN2
                     /note="LRPCR LAR3_EN2"
     intron          10203..11077
                     /vntifkey="21"
                     /dnas_title="mouse En2 intron"
                     /label=mouse En2 intron
                     /note="mouse En2 intron"
     primer          complement(10167..10190)
                     /vntifkey="27"
                     /dnas_title="FRT3_oligo"
                     /label=FRT3_oligo
                     /note="FRT3"
     misc_feature    10155..10202
                     /vntifkey="21"
                     /dnas_title="Frt"
                     /label=Frt
                     /note="Frt"
     misc_feature    14468..14475
                     /vntifkey="21"
                     /dnas_title="polylinker"
                     /label=polylinker
     misc_recomb     14436..14467
                     /note="Rox(inv)"
     misc_feature    14428..14435
                     /vntifkey="21"
                     /dnas_title="RE sites"
                     /label=RE sites
     primer          14368..14386
                     /vntifkey="27"
                     /dnas_title="seq primer NF"
                     /label=seq primer NF
     primer          14242..14265
                     /vntifkey="27"
                     /dnas_title="LRPCR RAF5"
                     /label=LRPCR RAF5
     misc_feature    14185..14427
                     /vntifkey="21"
                     /dnas_title="SV40 pA"
                     /label=SV40 pA
     misc_feature    13217..14161
                     /note="ERT2"
     misc_feature    15675..15675
                     /note="G to C conservative mutation to kill RsrII site"
     misc_feature    16023..16266
                     /note="SV40pA"
     misc_feature    16081..16104
                     /note="RAF5"
     misc_feature    15830..15849
                     /note="neoBstB1_5"
     misc_feature    15537..15555
                     /note="neoSphI_5"
     misc_feature    15200..15219
                     /note="neoPstI_5"
     misc_feature    15031..16016
                     /note="neomycin resistance "
     misc_feature    14881..14900
                     /note="betact5"
     promoter        14485..15005
                     /note="human beta actin promoter"
     insert          14182..16261
                     /source="pL1L2_GTIRES_BetactP FLFL MapVerified"
     misc_feature    16262..16266
                     /note="for bActNeo"
     misc_recomb     16268..16299
                     /note="Rox"
     misc_feature    16312..16332
                     /note="B2 Gateway"
     misc_feature    10091..16362
                     /note="Synthetic Cassette"
     exon            16714..16921
                     /db_xref="ENSEMBL:ENSMUSE00000186999"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 1 ENSMUSE00000186999"
     misc_feature    16363..17125
                     /note="Critical Region"
     misc_feature    17149..17182
                     /note="Downstream LoxP"
     misc_feature    17126..17205
                     /note="synthetic loxP region"
     misc_feature    17206..20955
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggaaccaca acgtttcaaa ggctgggatc ttacttgctt cttaccactt
     4321 tctttgtgcc tggcacacac caggagtcca acagggattg gtcatgtgag gagagagacc
     4381 tctgtggaag tcagaccaat ggtacatggg gctcctcgga atgaatatac attaggtgag
     4441 aagatgctga tgatgcttgg tgtccgaggc agcgactggt ggaaagggac tggagtgggg
     4501 agtgaggtac atggtgttag agttctgcct tgccgtttgc cagctgtgtg gactcttagg
     4561 gaaacgactc caccttgttt cctcagctgt gaatctgaga taacaagctg taggcagagc
     4621 aaatgaacta acaatgcgtg agactgggct gagaacagtg cccggagggc tatggtcgct
     4681 cagtcaatac tgagcacgaa aatacacaga cagaagcgaa cttgagaatg ttcacaacaa
     4741 tgtcaccata caaagggctg aggaccacat gggaagtagc atccaaatta ataacagact
     4801 ttatgagagg agccaaagga gagattggaa agaagaccaa cattttcatg actttctccc
     4861 caaactactg agcagcactg tgtacagagc ttcacttctt gatttccaga tccacaggct
     4921 ctggagacag ggcaaacaaa tgtagggcca ttgccagggg aaagctctga ggtaaaaccc
     4981 agaacttaaa atgatgcatt ttgggcaagt taaggcaaac taatatctct ggcacaatct
     5041 tctctcctgg tatgatgttc agactaggca ttctttaatt aaatatgcaa gtgtatcaag
     5101 gcaaacagaa gctccaccca cgtttcctgg aaggatatta gacagcagat agtaaaattc
     5161 tatagaaaaa agatttttca tgttttaaaa atttgaccac tcttgcctct catgtgatag
     5221 tgttacatat ttactgtggt tttgttacaa ctttttttta ttaaaaaaaa aaccagattt
     5281 tatttaattt agcaaacatt taaaagaaag cacagttgaa acaaatgtga gtcaaaaata
     5341 aacaaccact tacatcgatc aaaaatgaga ccattttttg atgacagatg ataaagaata
     5401 ctactgataa aatgggaaaa cctagtgcta aatgctatga aatggatctg tttatttcct
     5461 tcccataaac tctcaaattt ccttcccagg caatataggc catgaatatc accatttaaa
     5521 aacttacttc cacttttttt ctttatttat tacgtatttt cctcaattac atttccaacg
     5581 ctatcccaaa agtcccccac aagctccccc accctcccac tcccacttct tggccctgta
     5641 ctgaggcaca taaagtttgc acaaccaatg ggcctctctt tccactgatg gccaactagg
     5701 ccatcttcta ctacatatgc agctagagac acgagctctg gggggtactg tgtagttcat
     5761 attgttgttc cacctatagg gttgcagatc cctttagctc cttggttact ttctctggct
     5821 cctccattgg gggtcctgtg atccatcctg actgtgagca tccacttctg tgtttgctag
     5881 gccatagcat agtctcacaa gagacaacta tatctgggtc ctttcagcaa aatcttgcta
     5941 gtgtatgcag tggtgtttgg aagctgatta tgggatggat ccctggatat ggcagtctct
     6001 agatggtcca ttctttcgtc tcagctccaa actttgtctc tgtaactcct tccatgagtg
     6061 ttttgttccc aattctaaga aggggcaaag tgttcacact ttggtcttca ttcttcttga
     6121 gtttcatgtg ttttgcaaat tgtatcttat atcttgggta ttctaagctt ctgggctaat
     6181 atccacttat cagtgagtac atattatgtg agttcttttg tgattgggtt acctcactca
     6241 ggatgatgga ggaatatcac tttaaggtta gtttctcaga accttagatt tttctatgct
     6301 agtgtaatct atttaaccat ttattcttgt tctgcattta aactagtcac atacatataa
     6361 agtttgactc taattcctga gtcccgaatc ctgaccaaag agtacttatt tttttccttt
     6421 tcttttcttg gatattttat ttatttacat ttgaaatgtt atcccctttc ccagtccccc
     6481 cactccaaac tccctatcct attcccctcc ccctgcttct gtgagggtgt tcctccacct
     6541 actcactcac ccactcctgc ctccttgccc tggcattgcc ctgcactggg gcattgagcc
     6601 ttcatggggc caaagtcctc tcctcccact gatgtctgac aaggccatct tctggtacat
     6661 atgcagctgg agccatgggt tgctccatgt gtactctttg gttggtggtt aagtccctgg
     6721 gagctctggg ggggggggga gggtctggtt ggttgatatt gttgttcttc ctacgaggtt
     6781 gcaaactcct tgagcttcaa attacaagag cactttctac agaagaccaa cctttagttt
     6841 taaactctgg cattgaaatc ccagtgtcta aatccatgag ataggatcag ggacaaaccc
     6901 agtctgatct agaaaactgc tctgctgggc tctgacagtc atgagactgt caggagcacc
     6961 gtgctctcaa agcttagcat cctcttgaat ggaagatctc tgttattcaa tgcatactct
     7021 gacatctgtt attgcaccat ttctaactag aaacagtgtc ttagactgaa ggcaccagtg
     7081 agactcaaca gctcaccaga aatacgctgg atcctgtact agttttcctc cttccgggtc
     7141 caattttcag attctcctcc ccagccttgc acttgatgtg acttcttcaa aaggaaacac
     7201 aataatcaaa ccatgatgtc tataaggaac actaaggcca attatgtctg ccgtgttttg
     7261 tatgtgctct cgtgcgtcag tcctgacctc taatttccat gggactcact actaagttgt
     7321 ctcaggaaag aaaaataggc cctgcttgtt ctctgcccta tgaaaagcaa gtttgatgtt
     7381 gtagtagctt aaatgagccc cttttcaaat ggctggtctg catccccaca gagaagaagt
     7441 gacttttcat ggtgcacagc atgtagctgg cttaggaaat caatttctgc tggagagaat
     7501 gtgtacacac acattttcaa agacaatgga gtattctagg aagaagtaat atttgagttg
     7561 tatgttgagg caggagggat aataagcaat agacaccatt gtgtgcatat gaaagagttg
     7621 aagttaatat ccttagaaaa taattccttt ttatgtttgt tggccatgag tggattctta
     7681 gctaggtctg ttgaatattc ttgcccaacc ttaagtttta cagacttccc tcctcccttg
     7741 caattataaa gcaatctcca tggtcaatgt accagccagc actgtcttcc tagacgccat
     7801 ctgattccaa cctaaaatgt atatgtcttc accatcaaaa tgggcacttt ctcagtgtgt
     7861 ctttcattct tgttctctgt acattaagtt cttgcctatt ttgggtcctt tggggtttgc
     7921 tgtgctatta acaggtatgg aataccaagg aattgggcag agtacttaaa tcccagcttc
     7981 atgcctgtta ctaactttgt atgtttctgt aagccatttt taaatatttt cgtctgccta
     8041 tttatctgcc tatatattat ctttctatct aactacatat gtatggaagc tgtacctttt
     8101 tcatccataa ttagaaaatt tgtgctgtag tgttaggctt gaattttatg atggaattgt
     8161 gctttggaaa attagtctag gggtataaga aattgttctt tatgtctatg taagttattt
     8221 gagattttaa agattttata gtcataattc atcattacta tagaattctg cagagagtac
     8281 caccaacaca aaggccctta ctagatgtga tcctacccta cccccatttt tgagcaccca
     8341 gaaacatggg gcaaaatata cttcttttct ttgtaactta ctctacaaaa tgctggtatc
     8401 agcaacagaa aaatggtctg acagatacac aggcattgtg acatattctt gccagccttt
     8461 cctctctagg gacattaata attagttttt ggaaactact tttaatatat taactctgat
     8521 gtgcatccag gtagaagcta ctcagaagtc tagcatttgg tcttcacagc tgtcctggaa
     8581 ggaaggcttt agtcttctga aattgaattt cctcttattt agtataggcc agatagatgt
     8641 ctcttctctg ggttgtggaa gtgacatagc tatggcccag gaggtatggg tgctctttct
     8701 ggatctcagc acccaccaag ctggttctca gcaaatggca tatgtctaga tggagaaaca
     8761 tttactctca gcttgttcac agaaacttca tgtagagtta tgtgtacaaa gtggatcctc
     8821 tatctccttg ttcttccaac ctacagtgtc tttttttcac actgaagtgt gttttaactt
     8881 tcattcccaa gtagattgag tgatatattc atgaaacctg ttcagaatgt cccatctctg
     8941 ttctgtgtgt atgtgtgtgt gtgtttatgt tccagtatct atttgaacaa tttctttctc
     9001 tgcaagttct tcattctcat cttgtatttt gaagataagg aggcacatga aagtattgtg
     9061 gctttaactt ctttgtcatg tttacattaa ggttctgtgt atagagacat ttatttccag
     9121 ctgccttcca aaatgggaac cacattttga tcttgtgcca ctgagcttag catatttgag
     9181 tgatcaatga atatcctcta aaaatgggag atggtatctt ttgaagaagc atagggccct
     9241 ttgatcaaac aactacattt ggggggaggg cttcctatag gaccttggag ggttgcagag
     9301 tagttggaag aaaagcggga taagaaatgt acttgaaaga agatagaatg atttcaaagt
     9361 agatccgagc tatgtctgaa agagtgggtg acgctaaact agaaagcaca tttatctctc
     9421 acatcttaca gatggagagt ggtgtttggc catcttacta aactgcaggc ttgtctgtca
     9481 acactggttt tgttaatatg aagttaacca tatgtatgga gagaatatta tcataatttc
     9541 tctggaaatg atttggtacc cagaagagat gttccagttt ttgctgaaaa atgtccctcc
     9601 tattttttcc tccattaaaa gaaagttaat catttcacta tatttctgaa gcttgtgaat
     9661 tttgtgtttt aaacattaac aactttaaat taatttaaaa cactaaatgg ttcttaaacc
     9721 aattcaagag tatagaataa aatgaagaaa agcctctcaa aattccattt tgtgaaccca
     9781 tgtcaacatt ttgatttatg taagagcaag cagacaagtt cttggggtgg tagaattgtg
     9841 tgggtcatgg aggagacggt gtctatcaga actgaccatc aaggaaagca agcttagagg
     9901 ttccaacgtg actgggaaac atgctcctag gctggattgc agatccgagc accagcgtat
     9961 gcatttgtta gactggtttc tgtcatcaag tgctgttttt acttattaga ttatgaccac
    10021 cattccatag gattcagtgg tcttccaaag catttcgcat atcgtagagt taagcattcc
    10081 attgtctgta aaggcgcata acgataccac gatatcaaca agtttgtaca aaaaagcagg
    10141 ctggcgccgg aaccgaagtt cctattccga agttcctatt ctctagaaag tataggaact
    10201 tcgaaccctt tcccacacca ccctccacac ttgccccaaa cactgccaac tatgtaggag
    10261 gaaggggttg ggactaacag aagaacccgt tgtggggaag ctgttgggag ggtcacttta
    10321 tgttcttgcc caaggtcagt tgggtggcct gcttctgatg aggtggtccc aaggtctggg
    10381 gtagaaggtg agagggacag gccaccaagg tcagcccccc ccccctatcc cataggagcc
    10441 aggtccctct cctggacagg aagactgaag gggagatgcc agagactcag tgaagcctgg
    10501 ggtaccctat tggagtcctt caaggaaaca aacttggcct caccaggcct cagccttggc
    10561 tcctcctggg aactctactg cccttgggat ccccttgtag ttgtgggtta cataggaagg
    10621 gggacgggat tccccttgac tggctagcct actcttttct tcagtcttct ccatctcctc
    10681 tcacctgtct ctcgaccctt tccctaggat agacttggaa aaagataagg ggagaaaaca
    10741 aatgcaaacg aggccagaaa gattttggct gggcattcct tccgctagct tttattggga
    10801 tcccctagtt tgtgataggc cttttagcta catctgccaa tccatctcat tttcacacac
    10861 acacacacca ctttccttct ggtcagtggg cacatgtcca gcctcaagtt tatatcacca
    10921 cccccaatgc ccaacacttg tatggccttg ggcgggtcat cccccccccc acccccagta
    10981 tctgcaacct caagctagct tgggtgcgtt ggttgtggat aagtagctag actccagcaa
    11041 ccagtaacct ctgccctttc tcctccatga caaccaggct cccaggtccc gaaaaccaaa
    11101 gaagaagaac gcagatcgca gatcgcagat ctctagagtc accgagttgc tttaccggat
    11161 gaagagggcc gaaacatact gtccaaggcc cttgctggca atccacccaa ctgaagccag
    11221 acacaaacag aaaattgtgg caccggtgaa acagactttg aattttgacc ttctcaagtt
    11281 ggcgggagac gtcgagtcca accccggacc ggtgcatgtg gaccccaaga aaaagcggaa
    11341 ggtggaccct aaaaagaaac gcaaagtgga tcctaagaaa aaaagaaagg tgcacgtgtc
    11401 catggtgtcc aagggcgagg aactgttcac cggcgtggtg cccatcctgg tggaactgga
    11461 tggcgacgtg aacggccaca agttcagcgt gtccggcgag ggcgaaggcg acgccacata
    11521 cggaaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cttggcctac
    11581 cctcgtgacc acactgacct acggcgtgca gtgcttcagc agataccccg accatatgaa
    11641 gcagcacgac ttcttcaaga gcgccatgcc cgagggctac gtgcaggaaa gaaccatctt
    11701 ctttaaggac gacggcaact acaagaccag ggccgaagtg aagttcgagg gcgacaccct
    11761 cgtgaacaga atcgagctga agggcatcga cttcaaagag gacggcaaca tcctgggcca
    11821 caagctggag tacaactaca acagccacaa cgtgtacatc atggccgaca agcagaaaaa
    11881 cggcatcaaa gtgaacttca agatccggca caacatcgag gacggctccg tgcagctggc
    11941 cgaccactac cagcagaaca cccctatcgg cgacggccct gtgctgctgc ctgacaacca
    12001 ctacctgagc acccagagcg ccctgagcaa ggaccccaac gagaagaggg accacatggt
    12061 gctgctggaa ttcgtgaccg ccgctggcat caccctgggc atggacgagc tgtacaagcc
    12121 tgcaggcgag ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccgggcc
    12181 gtccaattta ctgaccgtac accaaaattt gcctgcatta ccggtcgatg caacgagtga
    12241 tgaggttcgc aagaacctga tggacatgtt cagggatcgc caggcgtttt ctgagcatac
    12301 ctggaaaatg cttctgtccg tttgccggtc gtgggcggca tggtgcaagt tgaataaccg
    12361 gaaatggttt cccgcagaac ctgaagatgt tcgcgattat cttctatatc ttcaggcgcg
    12421 cggtctggca gtaaaaacta tccagcaaca tttgggccag ctaaacatgc ttcatcgtcg
    12481 gtctgggctg ccacgaccaa gtgacagcaa tgctgtttca ctggttatgc ggcggatccg
    12541 aaaagaaaac gttgatgccg gtgaacgtgc aaaacaggct ctagcgttcg aacgcactga
    12601 tttcgaccag gttcgttcac tcatggaaaa tagcgaccgc tgccaggata taaggaatct
    12661 ggcatttctg gggattgctt ataacaccct gttacgtata gccgaaattg ccaggatcag
    12721 ggttaaagat atctcacgta ctgacggtgg gagaatgtta atccatattg gcagaacgaa
    12781 aacgctggtt agcaccgcag gtgtagagaa ggcacttagc ctgggggtaa ctaaactggt
    12841 cgagcgatgg atttccgtct ctggtgtagc tgatgatccg aataactacc tgttttgccg
    12901 ggtcagaaaa aatggtgttg ccgcgccatc tgccaccagc cagctatcaa ctcgcgccct
    12961 ggaagggatt tttgaagcaa ctcatcgatt gatttacggc gctaaggatg actctggtca
    13021 gagatacctg gcctggtctg gacacagtgc ccgtgtcgga gccgcgcgag atatggcccg
    13081 cgctggagtt tcaataccgg agatcatgca agctggtggc tggaccaatg taaatattgt
    13141 catgaactat atccgtaacc tggatagtga aacaggggca atggtgcgcc tgctggaaga
    13201 tggcgatctc gagccatctg ctggagacat gagagctgcc aacctttggc caagcccgct
    13261 catgatcaaa cgctctaaga agaacagcct ggccttgtcc ctgacggccg accagatggt
    13321 cagtgccttg ttggatgctg agccccccat actctattcc gagtatgatc ctaccagacc
    13381 cttcagtgaa gcttcgatga tgggcttact gaccaacctg gcagacaggg agctggttca
    13441 catgatcaac tgggcgaaga gggtgccagg ctttgtggat ttgaccctcc atgatcaggt
    13501 ccaccttcta gaatgtgcct ggctagagat cctgatgatt ggtctcgtct ggcgctccat
    13561 ggagcaccca gtgaagctac tgtttgctcc taacttgctc ttggacagga accagggaaa
    13621 atgtgtagag ggcatggtgg agatcttcga catgctgctg gctacatcat ctcggttccg
    13681 catgatgaat ctgcagggag aggagtttgt gtgcctcaaa tctattattt tgcttaattc
    13741 tggagtgtac acatttctgt ccagcaccct gaagtctctg gaagagaagg accatatcca
    13801 ccgagtcctg gacaagatca cagacacttt gatccacctg atggccaagg caggcctgac
    13861 cctgcagcag cagcaccagc ggctggccca gctcctcctc atcctctccc acatcaggca
    13921 catgagtaac aaaggcatgg agcatctgta cagcatgaag tgcaagaacg tggtgcccct
    13981 ctatgacctg ctgctggagg cggcggacgc ccaccgccta catgcgccca ctagccgtgg
    14041 aggggcatcc gtggaggaga cggaccaaag ccacttggcc actgcgggct ctacttcatc
    14101 gcattccttg caaaagtatt acatcacggg ggaggcagag ggtttccctg ccacagcttg
    14161 atgaagatct gagctcttaa ttaatgatca taatcagcca tatcacatct gtagaggttt
    14221 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
    14281 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
    14341 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
    14401 tcaatgtatc ttatcatgtc tggatccgtt taaactaact ttaaataatt ggcattattt
    14461 aaagttacat atggcggcca attcgccttc tgcaggagcg tacagaaccc agggccctgg
    14521 cacccgtgca gaccctggcc caccccacct gggcgctcag tgcccaagag atgtccacac
    14581 ctaggatgtc ccgcggtggg tggggggccc gagagacggg caggccgggg gcaggcctgg
    14641 ccatgcgggg ccgaaccggg cactgcccag cgtgggcgcg ggggccacgg cgcgcgcccc
    14701 cagcccccgg gcccagcacc ccaaggcggc caacgccaaa actctccctc ctcctcttcc
    14761 tcaatctcgc tctcgctctt tttttttttc gcaaaaggag gggagagggg gtaaaaaaat
    14821 gctgcactgt gcggcgaagc cggtgagtga gcggcgcggg gccaatcagc gtgcgccgtt
    14881 ccgaaagttg ccttttatgg ctcgagcggc cgcggcggcg ccctataaaa cccagcggcg
    14941 cgacgcgcca ccaccgccga gaccgcgtcc gccccgcgag cacagagcct cgcctttgcc
    15001 gatcctctag agtcgagatc cgccgccacc atgattgaac aagatggatt gcacgcaggt
    15061 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
    15121 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
    15181 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
    15241 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
    15301 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
    15361 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
    15421 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
    15481 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
    15541 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
    15601 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
    15661 cggctgggtg tggccgaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
    15721 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
    15781 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
    15841 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg
    15901 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
    15961 agcgcgggga tctcatgctg gagttcttcg cccacccccc ggatctaagc tctagataag
    16021 taatgatcat aatcagccat atcacatctg tagaggtttt acttgcttta aaaaacctcc
    16081 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
    16141 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
    16201 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
    16261 ggatccctaa ctttaaataa tgccaattat ttaaagttag gaattaattc acccagcttt
    16321 cttgtacaaa gtggttgata tctctatagt cgcagtaggc ggccaggatt attttcttat
    16381 gataagttct tttaagtggc attggaatgt caaagaacat ggatatataa acaccaggaa
    16441 gcatattagc cttcccaggt aggggatata cagcagcttc ctacgggaga attgtctaca
    16501 ttttttaagg aaacgcctgt tcccccttaa taaatatcac taagctgatt tcattgttgt
    16561 actgacatat acttatgtgc ataattgtct atgtgatagt tgtactataa tataggtgta
    16621 aggcatatga agtccttaac ctggcatgtt atgtaagctt acagatacag atttcttatt
    16681 cgacttctaa ctgtgagtgt ctccacttcc caggcacctg catgaacatc actcacagcc
    16741 agtgtcaaat tctgccctac cacagcacgt tggcacctct cttgccaatt gtcaaaaaca
    16801 tggacatgga gaagttcctc aagttcttca cgtacctcca tcgcctcagt tgctatcaac
    16861 atatcctgct cttcggctgt agcctcgcct tccctgagtg cgttgttgat ggcgatgaca
    16921 ggtattttgg agccatatca ttccgtgata agagcgcagt tattctggag acaagaggga
    16981 caaatcatcc tgttattaac tttccttttt tattgctttt agggggagaa gggacccaca
    17041 tctgtcatag ttaattgcga gtctctgtat ttagaaaatc cagattcatg ttttacggga
    17101 agaaattatc catattttag tggttgagat ggcgcaacgc aattaatgat aacttcgtat
    17161 agcatacatt atacgaagtt atggtctgag ctcgccatca gttcaatagc tgaaatgttt
    17221 gttagtgagg tttttttaaa tggaggtatg caaggggggt ggtaggggac aattaagtaa
    17281 taagaagtat caaggcctca tgatgagaca atgtagtcct actgccaaag gcttaaagag
    17341 aagaaaaggt tagaagcaat tggatacttg tagctaagag tgaagagaag ggataggtag
    17401 cccaactccc ttctccaatc atcctttctg ccagtaaatt tactcaccag cagggcacaa
    17461 aaacatccag tgcaatggat gaaacccaga atctcattaa atatcatcag ccaagaagtc
    17521 caaaaatctt atttaaatca attatgtgtg gccttcgggg tggtttttct catcagcatg
    17581 gggacctagt gctggactgg ggagtagatg tggagggaca aacagagact tttcaataag
    17641 atagagatca ttttccatga cagttgatca acccaaataa agcacatgct aagtacctat
    17701 ttaaatataa gataagttat tttctggatt gaaatattag gaactttcca gaatagtaat
    17761 tcgggtatga ctccatccca tgttgtctgt gacaatttca gcagtgtgtg aagtacccta
    17821 agcatcagag aaacacccgt tactttggtt gcaagctgtg gcgctgcctt tgtgtggaga
    17881 tggaacagaa gcagtagagg cagaggcgtg cagaatttgg tgaaacttga atgatcaagg
    17941 tgacttcatg gtacacacac agagagaagg tggattggat ctttcccatg ggttaaaata
    18001 tgagatgcct gtgtgggctg tggttgtgag tcagtgccat agtgcttgtc tcgctcacgg
    18061 agagcccaga actacagaaa caattgaaga tggccaccaa gagatggttt attttcccag
    18121 actgtggcaa cacagttggt ggttgtattc attttcttgg gttgctttaa ccaattgcca
    18181 cctgctgggt ggctcaaaca acagaaatgt cttccccctt agttctgaaa gctgaaagtc
    18241 tgggtgtcgg cacgctgtct ggttcccaga actctatggg gagaatccat tttccctctt
    18301 gcccatccca tttcctatta tttctgttcc tttgtcttgt gcccacgtcc ctccattttt
    18361 ggcctctgcc atcagagaac atgtcctgac tgccgacacc tcttcctctt ctgtctccta
    18421 taaggacact gtcattggat tcagagatca cctaacagtc tatgataata gcttcacatc
    18481 cagactctta attgtatccc acaaagatcc tatgtccaat gtgttctaga gattcccggt
    18541 tctagagatt aagatgtgga cctctcactt catagcagtg gttgggaaag caggctaaag
    18601 gagtcgtgtg tcgtgacctc gtcatccata aaaagagaaa taatagttac acttatgtta
    18661 aaaaattaat gtgagtattt aatgacatgt ggggcttggg acactgcctg gcccttagaa
    18721 agagctcaat aaaggttagc aataactctg aagtggtgat ttgtgaagga caagcagaat
    18781 taatgaagtg gagactacaa aaatgtactt ctaatgtgtg tgtgtgtgaa ggattaccaa
    18841 aaaaaaaaaa gtaaatgaag caaaattaga tgaaatatga aaggattttg gagctcgatt
    18901 ctcattctgt ttctgtttca tttccaaaaa tttccactaa tatatatatg tatcagatta
    18961 actcctttgt gggttcatgc atgcacacac aaagaaaatg gaagggagtg tgttcagagt
    19021 cctggaaccg ctgggtccag catggcgacc ctctttattc taactcagtt ctgcctgggt
    19081 aaacgctttt gagctgccca ctcttttcag ggcactttaa attaagtaca cgtccccccc
    19141 tgacctgatt tagcctttga taactatacc agtatgagag tttttccatt ttctgttata
    19201 atgaataata atttacagtt acttttcaag tgttctttgg ggacttatcc tatttttatt
    19261 attatattta gttttctaat tgtactttta ggccatgttt cctgctgtca tttagtgtgt
    19321 acatgggaac tgcagcctaa tttaataagc agaataagtt cagcaaagta aatgtattat
    19381 ataaagccat tataaggtca acaatatttt tcttgtgttt cactattctg tatcatctat
    19441 tataggctgt attatgaaaa ttaagaaaaa gtgtttgttt tccagcctag aaaaaatagc
    19501 ttttaataac aattaaaatt taattttaaa agattatagt gagatatata aacaggatag
    19561 tttttaaacc tgattctttg gagaccacag ttactctttg actacattgt acgttgaaac
    19621 aatggaattt aattgtcttt attttgaact gtgtttgctt tgaccaaatg ggtatggttg
    19681 tattgcaaca gggtctattg aaaggcagga aagcatatgg attcagggaa gcatataatc
    19741 taaaaatata aaattttaac cgataaacta gtggaacggc tctaaagtac gtaggttaga
    19801 atatatttct taaaaatatc aagtagatag caaaatgttg gttagtgttt agatgacatg
    19861 tttgatttcc tgattttctc aaatggacag tgtgtgtgtg tgtttgtgtg tgtgtgtgtg
    19921 tgtgtgtgtg tgtgtgtgtg tgtagtattg cttgtttgtg gaagccaggg gtcaacctca
    19981 gtatcattcc tcaggaacca gctgccatgg tttctgaacc tgtccttggt ttgggacttg
    20041 ccaagcagac agcctggcca gtcagtgagc tccagacatt cccctgcctc cactcctcag
    20101 ggctaggctt acaagcagga ggcaccacgc ccacttcggt tttttttaaa cactagttcc
    20161 aagaattgag ctgagattct tgtgcttatg tggcaagcaa ttctctggct gagctatttc
    20221 cgcagtctag actagaacta actttttaag tgtaatagtc tttagtaata taaagttata
    20281 caaatttaag aaatagtaaa accttgctga gactaaccct tccccccgca aagtgactta
    20341 cagttctgat cgtgcctccc actttcttac gacttatagg aggtgctctg gtatctaccc
    20401 tgtccagcag ataaattctt tgtaaatcgc agttcttgtt gttgatggag tatgtgactt
    20461 tctgggctga tatccactta atcccgtggt tagaaatgcc agctttctat tttcctcatc
    20521 gtgatcattt cagaacagga tagcatttga aaaatgaaga aaatatctaa taaaaaataa
    20581 attaattaaa aaaaaataaa agaacaaatt gaccgagatg ccatccattt gtggctttcg
    20641 ttagacttag atttcaaact aagctccatg ccacagcttt tgaagtcctt cttgcctctc
    20701 tagcgtcatg gatccagcct caccttcagt gaccttcctt tccatttctc aaacaaacaa
    20761 aaaaacacaa caaaaccctg aaaccgtctt ctgaacagcc ttgtatgtgc atgctgttcc
    20821 ctttgcctac agaggcccct cctctcccat caaggccgtg gcttccaagt cttggctgga
    20881 gtgaagccct ctctaaacac ccggattctc ttgttttctc atggcagcct ggtgaagccc
    20941 cttgttttca ccata
//