LOCUS       211393#pL1L2GT1_LF2A_nEGFPOT2A_CreERT2_pAbActNeopA#L3L4_pD223_DTA_T_spec        23359 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2GT1_LF2A_nEGFPOT2A_CreERT2_pAbActNeopA
COMMENT     design_id: 211393
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000098669
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            9355..9714
                     /db_xref="ENSEMBL:ENSMUSE00001027988"
                     /rank="2"
                     /note="ENSMUSE00001027988"
     exon            8433..8587
                     /db_xref="ENSEMBL:ENSMUSE00000475696"
                     /rank="1"
                     /note="ENSMUSE00000475696"
     misc_feature    4275..10727
                     /note="5 arm"
     misc_feature    10755..10779
                     /note="B1 Gateway"
     misc_feature    11746..11763
                     /note="FCHK QC critical region"
     misc_feature    11730..11745
                     /note="FCHK QC prefix region"
     misc_feature    11764..11769
                     /note="FCHK QC suffix region"
     misc_feature    11892..11943
                     /note="F2A"
     misc_feature    11769..11891
                     /note="1Dleader"
     primer          11615..11636
                     /vntifkey="27"
                     /dnas_title="seq primer FCHK2"
                     /label=seq primer FCHK2
                     /note="seq primer FCHK2"
     exon            11715..11768
                     /vntifkey="61"
                     /dnas_title="mouse En2 exon"
                     /label=mouse En2 exon
                     /note="mouse En2 exon"
     misc_feature    12815..12815
                     /note=" A to G to kill RsRII site"
     misc_feature    12765..12818
                     /note="self-cleaving' 2A peptide"
     misc_feature    12819..13844
                     /note="Cre"
     misc_feature    11949..12038
                     /note="3X NLS"
     misc_feature    12039..12755
                     /note="EGFP"
     primer          complement(10908..10929)
                     /vntifkey="27"
                     /dnas_title="LRPCR LAR3_EN2"
                     /label=LRPCR LAR3_EN2
                     /note="LRPCR LAR3_EN2"
     intron          10840..11714
                     /vntifkey="21"
                     /dnas_title="mouse En2 intron"
                     /label=mouse En2 intron
                     /note="mouse En2 intron"
     primer          complement(10804..10827)
                     /vntifkey="27"
                     /dnas_title="FRT3_oligo"
                     /label=FRT3_oligo
                     /note="FRT3"
     misc_feature    10792..10839
                     /vntifkey="21"
                     /dnas_title="Frt"
                     /label=Frt
                     /note="Frt"
     misc_feature    15105..15112
                     /vntifkey="21"
                     /dnas_title="polylinker"
                     /label=polylinker
     misc_recomb     15073..15104
                     /note="Rox(inv)"
     misc_feature    15065..15072
                     /vntifkey="21"
                     /dnas_title="RE sites"
                     /label=RE sites
     primer          15005..15023
                     /vntifkey="27"
                     /dnas_title="seq primer NF"
                     /label=seq primer NF
     primer          14879..14902
                     /vntifkey="27"
                     /dnas_title="LRPCR RAF5"
                     /label=LRPCR RAF5
     misc_feature    14822..15064
                     /vntifkey="21"
                     /dnas_title="SV40 pA"
                     /label=SV40 pA
     misc_feature    13854..14798
                     /note="ERT2"
     misc_feature    16312..16312
                     /note="G to C conservative mutation to kill RsrII site"
     misc_feature    16660..16903
                     /note="SV40pA"
     misc_feature    16718..16741
                     /note="RAF5"
     misc_feature    16467..16486
                     /note="neoBstB1_5"
     misc_feature    16174..16192
                     /note="neoSphI_5"
     misc_feature    15837..15856
                     /note="neoPstI_5"
     misc_feature    15668..16653
                     /note="neomycin resistance "
     misc_feature    15518..15537
                     /note="betact5"
     promoter        15122..15642
                     /note="human beta actin promoter"
     insert          14819..16898
                     /source="pL1L2_GTIRES_BetactP FLFL MapVerified"
     misc_feature    16899..16903
                     /note="for bActNeo"
     misc_recomb     16905..16936
                     /note="Rox"
     misc_feature    16949..16969
                     /note="B2 Gateway"
     misc_feature    10728..16999
                     /note="Synthetic Cassette"
     exon            20080..20355
                     /db_xref="ENSEMBL:ENSMUSE00000965665"
                     /type="targeted"
                     /rank="5"
                     /note="target exon 1 ENSMUSE00000965665"
     exon            18101..18355
                     /db_xref="ENSEMBL:ENSMUSE00001033065"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 2 ENSMUSE00001033065"
     exon            17349..17633
                     /db_xref="ENSEMBL:ENSMUSE00001040600"
                     /type="targeted"
                     /rank="3"
                     /note="target exon 3 ENSMUSE00001040600"
     misc_feature    17000..20635
                     /note="Critical Region"
     misc_feature    20659..20692
                     /note="Downstream LoxP"
     misc_feature    20636..20715
                     /note="synthetic loxP region"
     misc_feature    20716..23359
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggaaatatt taaacaatat gcaataacat tatgcctaca gagagagtgt
     4321 gggggggaag agaaacacac actcattgct ggtgggtgtg caaattaata gccattatac
     4381 aaagctgtgt ggaagtctct taaaaaatag caataccata taacgcagct atttcactgg
     4441 acatataccc agagaacctg taccctacca ttgagatatc tgcactccca tgattacagc
     4501 tgctccatcc catatagcaa ggaagtagaa ccaacctaga tctccatcaa tatatgaatg
     4561 cacaatacaa gtctaccaca cactcacaca tacacacaca cacacacaca cacacactca
     4621 cacatacaca cacacacaca cacacacaca cacactgtga agtattgttc gtctctacag
     4681 aaagatgaat cacaaagttt acagggatgt ggatagactt agaatatatc atgttaaatg
     4741 agatcatttg atctcaggaa gaaaacccca tatgtccttt cttgtttgtg gatcctagct
     4801 ctaatgtctg catgtacaca tgtaattaca tatataacat taggaaggag aacatgaagg
     4861 ggtttggtct ggtttggttt ggtttggttt ggtttggttt tcaagacagg gtttctctgt
     4921 gtagccctgg ctgtcctgga actcactctg tagaccaggc tggcctgaaa ctcacagaga
     4981 tcctccttcc tctgcctccc gagtgttgga ggtgtgcgcc accgcctggc aatggtgtgg
     5041 tagttctaag gagacaaagt gctccctcat gggctcacac atatgatgta cctggtgtgg
     5101 gccctttgga tgctctctcg catgtaaccg gttttgacgt aaaacaggcc cttagcaagc
     5161 agtgtccacg tgggactcca tgcggggctg ctgccttact gggaggcctg gatctgaagt
     5221 tctgtctttc atcctttgaa tattcctgta cagtgtagtt cttgtaagta ccaacaatta
     5281 gggcccatag ttctttaaac aagaatttca cctataaaaa ctatagagag tgaagacact
     5341 cataagtatg cttaaataat cataattatg aatttataaa acaaattcca aggaatgctg
     5401 actcactgct actgcatctc aaatactaac acaccaggaa ttacacacac taagaagctt
     5461 cctttttgtg gtttttaatg ttacaattct ttttttaata tatgtatata atttttatta
     5521 ggtattttcc tcatttacat ttccaatgct atcccaaaag tcccccatac catccccccg
     5581 actcccactt tttggccctg gcgttcccct gtactggagc atataaagtt tgcaagtcca
     5641 atgggcctct ctttccagtg atggccgact aggccatctt ttgatacata tgcagctaga
     5701 gtcaagagct ccggggtact ggttagttca taatgttgtt ccacctatag ggttgcagat
     5761 ccctttagct ccttgggtac tttctctagc tcctccattg ggggccctgt gatccatcca
     5821 atagctgact atgagcatcc acttctgtgt tttctaggcc ccggcacagt ctcacaagag
     5881 acagctatat cagggtcctt tcagcaaaat cttggtagtg tatgcaatgg tgtcagtgtt
     5941 tggaggctga ttatgggatg gctccctgga tatggcaatc tctagatggt ccatcctttt
     6001 gtctcagctc caaactttgt ctctgtaatg ttacaattct aaaatatgct attgttggtg
     6061 aatgaaagtg ctttgcttta aaatttagaa gtcagaataa agcaattata gagaaagcac
     6121 agttctagga tattgctgcc caggaacaag atgtacaact aagttactag acacatggga
     6181 atgggagatc aacctcatta gtcaccgtgg aactgcagat caaatggcag tcagtctcat
     6241 ttcacagata taatagtctc tagctaaaaa aaaaaaaaaa aggaatggat cctgaaaaga
     6301 tctatacact tgggatcttt cctggaaacc ttagtgctca actcctccac agttttgaaa
     6361 gccttgttac catccttagg gcaacccaga tgtgagctgc aggtctcctc ctgaacacca
     6421 gggggcgaaa ttggaccata taatcaacag aaaaaactcc tgagccatga ccaccccttg
     6481 tatattttct ttatttacat ttcaaatgtt ttcccctttc caggtctccc cttcagaacc
     6541 cccctatcct tacctccctc cccctacctc tatgaggatg ctcccacatc cacccattct
     6601 cattctcctg ccctggcatt cccctacact ggagtattga acagcctcag gcctgagggc
     6661 ctctcctccc actgatatcc aataaggcca tcttctgcca catgtttggc cagggccatg
     6721 tgtcactaat ggatacagaa aatgtggtac agccgggcgg tagtggcgca cacctttaat
     6781 cccagcactt gggaggcaga gacaggcgga attctgagtt cgaggccagc ctggtctaca
     6841 aagtgagttc caggacagcc tagactatac agagaaaccc tgtctcgaaa aacaaaaaag
     6901 gaagaaagaa agaaagaaag aaagaaagaa agaaagaaag aaagaaagaa agaaagaaag
     6961 aaagaaagaa agaaagaaaa tgtggtacat ttacacaaca gactactaat cagccattaa
     7021 aaacaatgac ttcatgaaat tcataggcaa atggatggaa cttgagaata tcatcctgag
     7081 tgaggtaact cagtcacaaa ggaacaaaca aacaagatat gtactcactg ataagtggat
     7141 attagtccaa aagttcagaa tacccaagat tcaattcaca gactatatga agcctaagaa
     7201 gaagaaagac caaagtgtgg atgcttcact gcttcttaga agggtgaaca aaatcttcac
     7261 aggaggaaat acggagacaa agtgtggagc aaagactgaa ggaaaggcca tccagagact
     7321 gccccacctg gggatccatt caacatatag ccaccaaacc tgtacacgat tgtggatgtc
     7381 aggaagtgct agctgatgga agccggatct gtctgtctcc taagaagctt taccagagcc
     7441 tgacaagtac agaggcaagg cgaaagctca cagtcaacca ttggactgag catggggtcc
     7501 cagatggagg agttggagaa gggactgaag gagccgaggg gggtttgcag cccagtggag
     7561 ggagcaacag tgccaagagg ccagaccccc acagagctcc cgggatctgg atgaccaacg
     7621 aaagaatacg caagaaccta tcttgcaacc tggattgcag tttccatccc agttgacaca
     7681 cctggagcca gctccctcac ctaaccatac tctgtcctcc tggctctttc ctgtatctct
     7741 gagaactcca ttctgcaagg acagccgatg tggtcccttc catcctccat catccatggg
     7801 agagatggga aatcccaggg cattcaccaa ggagaacaga gccatcctgg gacggttacg
     7861 gagggcacgg gcagacctga atcacatttg gccagcacag gaagctgggg aggtctccct
     7921 ggcaccctcc ataggaaggt ggagcacaca gtcctctttc caggacacac aggtcacctc
     7981 ctcctgcaca cccaggatat gaagcccctg agacaacttg tatcctagga tcagacacat
     8041 gactggtgtc atcagtgacg atggatcagg tcctacccag tcatcactca gctaggcctt
     8101 tccttaaccc tccagataac tctgccactt cctgcctgga gtaaacccca cctctgtgag
     8161 cattgagagc agggcacaga gggctcccat gggggtttgt gtcactctag gctacaggaa
     8221 atgctggaac tcctgctgca gttgacagcc ccaaggccag ggcacagggc actcctcagc
     8281 cttgctgctc ggagtatgtt ctagaacact gaactggaaa gaggaataga aggacgggag
     8341 gcccacactg acaggagttc agcattgtca gactcacagg ctccaccccc agcccacgtg
     8401 gatctgggag gtgccctccc ctgggaggag acaaagctcc tttaagaaaa gcagggcaga
     8461 tatcagggca gcctggctta gcagtagtgt tggagaagaa gctagcaggc aggcagcaga
     8521 gacatggagc tggcctcagc acatctccac aaagggcagg ttccctgggg aggactactg
     8581 ctcacaggta aggagatatt ccttcccagt agagagcagg ggagctcaga gactggctgg
     8641 gctcttctgg gagaggaaag gaacctgaga agggacatct ggcttctgct tgaagcttga
     8701 cggcaacagg aagctctcag tgagtgtgaa ttggctagtg gtgaggagta actcagttct
     8761 ttgctattgt taagtttatc gcactggcta ggatatcctg aagactgaag tccacaactc
     8821 tgtcagggtg actctccacg taaaaaccaa actggggcaa gtaaacagaa ttattgcata
     8881 ctacactgag aaatttgcaa atacactagg ctctgggaca ctgatttgca aacaggtata
     8941 tgagcatgtt ccaggcagca gggattaagc caaaacccac ctagccctgc acacaaagcc
     9001 ctagttctat gtgtaaaacg gcagacctgc gagggcatct aggtggggtc aggttggcaa
     9061 gcacttcaga aaaaatcaaa gcagagaaag gccagaaaat gaaggtgcag ggtctttaga
     9121 ggagggggtc agagaagata ggcctacact cagcaaagac tctgagtgtg agctggggtc
     9181 tgagggcagt gagaggtgag ctgtgtgaat gccggagaag agcgtttcct acagtggaaa
     9241 gatgaggaat ggaggtgatc tgctggccac cacccaatag gacacaggca cagcaaggct
     9301 gagaggtttt gcaaaggtcc taagattgat aggtctttct ctcttccctc ttagcctcac
     9361 ttttagcctc ctggagccct gccaccactg ctgaagtcac cattgaggct gtgccgcccc
     9421 aggttgctga agacaacaat gttcttctac ttgttcacaa tctgcccctg gcgcttggag
     9481 cctttgcctg gtacaaggga aacactacgg ctatagacaa agaaattgca cgatttgtac
     9541 caaatagtaa tatgaatttc acggggcaag catacagcgg cagagagata atatacagca
     9601 atggatccct gctcttccaa atgatcacca tgaaggatat gggagtctac acactagata
     9661 tgacagatga aaactatcgt cgtactcagg cgactgtgcg atttcatgta caccgtaagt
     9721 aattatctgt aacctcggcg tcatgggtag agttaatcct gcttcccaca cagcactgtc
     9781 aatccttgat gactcaacta tgtcccctac attgtggtgg gagcatttag ttcggggcac
     9841 acagagggga gaaatacagc aataaaccgc gatgcctcac ttggacccac tccttcagag
     9901 aagagattgt gaggtaactc agtgtgagga tgtcagactc tgcacagtaa cttaggctgc
     9961 ttatcctgag catggcctcg agaaagaccc ttcagggctc aagcagggcc tgcctgagga
    10021 aaccatgaga atctcacgga gaggtgagca ccaggaaact ttgctccaga cccagcctgg
    10081 actccaggaa ccctgggaag ttgttagcct gggttgagat ttgccctctt gttagagctg
    10141 acagggacaa cggttgattg taggctggta ggctgttgac agtcagccta gactagagcc
    10201 atgtctgagg taggtcacct gggtatctcc ttctgagacc cagtgtgctc tgcgtacatg
    10261 gagaggtatg aaagacaact agccaactgt tgtgatgttc ctgtgagact tcccaggaaa
    10321 atcgagagcc ctaaaatgat aggaggaaac cgtgtacaaa tggcagctcc ttgctctcca
    10381 gggtgtcaag cccagggatt atttcctgca gcaaatagta acagactctg gtctatggaa
    10441 ggtcagatca ttgctccttt taaggttcat ttgcaggcat ggcaatgatc atttaccata
    10501 tgtcctatta tggggaaact gaggcagtag atacagtcac tgagtcaggg tcacataggc
    10561 cctgggtggc atgagtttat ctgtccagag ccacatctcc agcctcccca ccctggagat
    10621 cttattaggc acttgttatt cttttagata atttcttgca ctcaggcttc tcttttgggg
    10681 gatctcttct tactcatgtc agtcaacacc aaacacccga caaaattaag gcgcataacg
    10741 ataccacgat atcaacaagt ttgtacaaaa aagcaggctg gcgccggaac cgaagttcct
    10801 attccgaagt tcctattctc tagaaagtat aggaacttcg aaccctttcc cacaccaccc
    10861 tccacacttg ccccaaacac tgccaactat gtaggaggaa ggggttggga ctaacagaag
    10921 aacccgttgt ggggaagctg ttgggagggt cactttatgt tcttgcccaa ggtcagttgg
    10981 gtggcctgct tctgatgagg tggtcccaag gtctggggta gaaggtgaga gggacaggcc
    11041 accaaggtca gccccccccc cctatcccat aggagccagg tccctctcct ggacaggaag
    11101 actgaagggg agatgccaga gactcagtga agcctggggt accctattgg agtccttcaa
    11161 ggaaacaaac ttggcctcac caggcctcag ccttggctcc tcctgggaac tctactgccc
    11221 ttgggatccc cttgtagttg tgggttacat aggaaggggg acgggattcc ccttgactgg
    11281 ctagcctact cttttcttca gtcttctcca tctcctctca cctgtctctc gaccctttcc
    11341 ctaggataga cttggaaaaa gataagggga gaaaacaaat gcaaacgagg ccagaaagat
    11401 tttggctggg cattccttcc gctagctttt attgggatcc cctagtttgt gataggcctt
    11461 ttagctacat ctgccaatcc atctcatttt cacacacaca cacaccactt tccttctggt
    11521 cagtgggcac atgtccagcc tcaagtttat atcaccaccc ccaatgccca acacttgtat
    11581 ggccttgggc gggtcatccc cccccccacc cccagtatct gcaacctcaa gctagcttgg
    11641 gtgcgttggt tgtggataag tagctagact ccagcaacca gtaacctctg ccctttctcc
    11701 tccatgacaa ccaggctccc aggtcccgaa aaccaaagaa gaagaacgca gatcgcagat
    11761 cgcagatctc tagagtcacc gagttgcttt accggatgaa gagggccgaa acatactgtc
    11821 caaggccctt gctggcaatc cacccaactg aagccagaca caaacagaaa attgtggcac
    11881 cggtgaaaca gactttgaat tttgaccttc tcaagttggc gggagacgtc gagtccaacc
    11941 ccggaccggt gcatgtggac cccaagaaaa agcggaaggt ggaccctaaa aagaaacgca
    12001 aagtggatcc taagaaaaaa agaaaggtgc acgtgtccat ggtgtccaag ggcgaggaac
    12061 tgttcaccgg cgtggtgccc atcctggtgg aactggatgg cgacgtgaac ggccacaagt
    12121 tcagcgtgtc cggcgagggc gaaggcgacg ccacatacgg aaagctgacc ctgaagttca
    12181 tctgcaccac cggcaagctg cccgtgcctt ggcctaccct cgtgaccaca ctgacctacg
    12241 gcgtgcagtg cttcagcaga taccccgacc atatgaagca gcacgacttc ttcaagagcg
    12301 ccatgcccga gggctacgtg caggaaagaa ccatcttctt taaggacgac ggcaactaca
    12361 agaccagggc cgaagtgaag ttcgagggcg acaccctcgt gaacagaatc gagctgaagg
    12421 gcatcgactt caaagaggac ggcaacatcc tgggccacaa gctggagtac aactacaaca
    12481 gccacaacgt gtacatcatg gccgacaagc agaaaaacgg catcaaagtg aacttcaaga
    12541 tccggcacaa catcgaggac ggctccgtgc agctggccga ccactaccag cagaacaccc
    12601 ctatcggcga cggccctgtg ctgctgcctg acaaccacta cctgagcacc cagagcgccc
    12661 tgagcaagga ccccaacgag aagagggacc acatggtgct gctggaattc gtgaccgccg
    12721 ctggcatcac cctgggcatg gacgagctgt acaagcctgc aggcgagggc agaggaagtc
    12781 ttctaacatg cggtgacgtg gaggagaatc ccgggccgtc caatttactg accgtacacc
    12841 aaaatttgcc tgcattaccg gtcgatgcaa cgagtgatga ggttcgcaag aacctgatgg
    12901 acatgttcag ggatcgccag gcgttttctg agcatacctg gaaaatgctt ctgtccgttt
    12961 gccggtcgtg ggcggcatgg tgcaagttga ataaccggaa atggtttccc gcagaacctg
    13021 aagatgttcg cgattatctt ctatatcttc aggcgcgcgg tctggcagta aaaactatcc
    13081 agcaacattt gggccagcta aacatgcttc atcgtcggtc tgggctgcca cgaccaagtg
    13141 acagcaatgc tgtttcactg gttatgcggc ggatccgaaa agaaaacgtt gatgccggtg
    13201 aacgtgcaaa acaggctcta gcgttcgaac gcactgattt cgaccaggtt cgttcactca
    13261 tggaaaatag cgaccgctgc caggatataa ggaatctggc atttctgggg attgcttata
    13321 acaccctgtt acgtatagcc gaaattgcca ggatcagggt taaagatatc tcacgtactg
    13381 acggtgggag aatgttaatc catattggca gaacgaaaac gctggttagc accgcaggtg
    13441 tagagaaggc acttagcctg ggggtaacta aactggtcga gcgatggatt tccgtctctg
    13501 gtgtagctga tgatccgaat aactacctgt tttgccgggt cagaaaaaat ggtgttgccg
    13561 cgccatctgc caccagccag ctatcaactc gcgccctgga agggattttt gaagcaactc
    13621 atcgattgat ttacggcgct aaggatgact ctggtcagag atacctggcc tggtctggac
    13681 acagtgcccg tgtcggagcc gcgcgagata tggcccgcgc tggagtttca ataccggaga
    13741 tcatgcaagc tggtggctgg accaatgtaa atattgtcat gaactatatc cgtaacctgg
    13801 atagtgaaac aggggcaatg gtgcgcctgc tggaagatgg cgatctcgag ccatctgctg
    13861 gagacatgag agctgccaac ctttggccaa gcccgctcat gatcaaacgc tctaagaaga
    13921 acagcctggc cttgtccctg acggccgacc agatggtcag tgccttgttg gatgctgagc
    13981 cccccatact ctattccgag tatgatccta ccagaccctt cagtgaagct tcgatgatgg
    14041 gcttactgac caacctggca gacagggagc tggttcacat gatcaactgg gcgaagaggg
    14101 tgccaggctt tgtggatttg accctccatg atcaggtcca ccttctagaa tgtgcctggc
    14161 tagagatcct gatgattggt ctcgtctggc gctccatgga gcacccagtg aagctactgt
    14221 ttgctcctaa cttgctcttg gacaggaacc agggaaaatg tgtagagggc atggtggaga
    14281 tcttcgacat gctgctggct acatcatctc ggttccgcat gatgaatctg cagggagagg
    14341 agtttgtgtg cctcaaatct attattttgc ttaattctgg agtgtacaca tttctgtcca
    14401 gcaccctgaa gtctctggaa gagaaggacc atatccaccg agtcctggac aagatcacag
    14461 acactttgat ccacctgatg gccaaggcag gcctgaccct gcagcagcag caccagcggc
    14521 tggcccagct cctcctcatc ctctcccaca tcaggcacat gagtaacaaa ggcatggagc
    14581 atctgtacag catgaagtgc aagaacgtgg tgcccctcta tgacctgctg ctggaggcgg
    14641 cggacgccca ccgcctacat gcgcccacta gccgtggagg ggcatccgtg gaggagacgg
    14701 accaaagcca cttggccact gcgggctcta cttcatcgca ttccttgcaa aagtattaca
    14761 tcacggggga ggcagagggt ttccctgcca cagcttgatg aagatctgag ctcttaatta
    14821 atgatcataa tcagccatat cacatctgta gaggttttac ttgctttaaa aaacctccca
    14881 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
    14941 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
    15001 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
    15061 atccgtttaa actaacttta aataattggc attatttaaa gttacatatg gcggccaatt
    15121 cgccttctgc aggagcgtac agaacccagg gccctggcac ccgtgcagac cctggcccac
    15181 cccacctggg cgctcagtgc ccaagagatg tccacaccta ggatgtcccg cggtgggtgg
    15241 ggggcccgag agacgggcag gccgggggca ggcctggcca tgcggggccg aaccgggcac
    15301 tgcccagcgt gggcgcgggg gccacggcgc gcgcccccag cccccgggcc cagcacccca
    15361 aggcggccaa cgccaaaact ctccctcctc ctcttcctca atctcgctct cgctcttttt
    15421 ttttttcgca aaaggagggg agagggggta aaaaaatgct gcactgtgcg gcgaagccgg
    15481 tgagtgagcg gcgcggggcc aatcagcgtg cgccgttccg aaagttgcct tttatggctc
    15541 gagcggccgc ggcggcgccc tataaaaccc agcggcgcga cgcgccacca ccgccgagac
    15601 cgcgtccgcc ccgcgagcac agagcctcgc ctttgccgat cctctagagt cgagatccgc
    15661 cgccaccatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
    15721 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
    15781 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
    15841 tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
    15901 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
    15961 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
    16021 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
    16081 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
    16141 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat
    16201 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
    16261 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg ccgaccgcta
    16321 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
    16381 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
    16441 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg
    16501 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
    16561 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
    16621 ttcttcgccc accccccgga tctaagctct agataagtaa tgatcataat cagccatatc
    16681 acatctgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
    16741 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
    16801 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
    16861 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccctaactt taaataatgc
    16921 caattattta aagttaggaa ttaattcacc cagctttctt gtacaaagtg gttgatatct
    16981 ctatagtcgc agtaggcggc agctgtcctg gaaaatggag ggagtcgaca gattgtgtct
    17041 aaaggcagac aaccagtcag tgagtaccag gacataagag gaggtctctg attctgagac
    17101 ttggatctgt gcatcaacaa actgcagctc agttactcag gagagagcct tgcttctccc
    17161 atacacagtg caggagaccc catcctctct gagcactcag atactccatc tgcctataat
    17221 ttgcctcctg cctggttctc taaggatctg ggttggcagg gaggtaagag accagcttgg
    17281 attgaaagag gccattgtaa agatcagagg taccacacag ggcaatcttc tctctattat
    17341 ctgcacagcc atattattaa agcccaacat cacaagcaac aactccaatc ccgtggaggg
    17401 tgacgactcc gtatcattaa cctgtgactc ttacactgac cctgataata taaactacct
    17461 gtggagcaga aatggtgaaa gcctttcaga aggtgacagg ctgaagctgt ctgagggcaa
    17521 caggactctc actttactca atgtcacgag gaatgacaca ggaccctatg tgtgtgaaac
    17581 ccggaatcca gtgagtgtca accgaagtga cccattcagc ctgaacatta tctgtgagta
    17641 acttcttccc catccttgct acagctgcct aaatacagag agctagagcc gggacacaca
    17701 ttcgcttctg ggttcatgac agacccacca catatatata tctaggtggg ccatgatatg
    17761 ctggcctagc ccaagcacat gaagccaggc gcagtcctga gctagaatga actggaggga
    17821 ttactcctat ctagagagga tcagggtgat ggagacacag gggacatctc agactcaggc
    17881 tcagcgaaca cttgagtgtc cagtggggct gttctctgtg tgttagtgat ggaccaggac
    17941 gcatgcacac tacatgagta cccttctatt aaccttcacc caggctctgg ctgggacctg
    18001 cttaactgaa gttatagcac agatcctgta gccttctaga gaccaggatt tccccttccc
    18061 tctgctgacg tcacatttgg ctttattctg tttgctccag atggtccgga caccccgatt
    18121 atatccccct cagatattta tttgcatcca gggtcaaacc tcaacctctc ctgccatgca
    18181 gcctctaacc cacctgcaca gtacttttgg cttatcaatg agaagcccca tgcatcctcc
    18241 caagagctct ttatccccaa catcactact aataatagcg gaacctatac ctgcttcgtc
    18301 aataactctg tcactggcct cagtaggacc acagtcaaga acattacagt ccttggtaag
    18361 tggatctctg aagtatcagc accagggttg gagtggagtc ttttggtgtt ctgggaaggt
    18421 gcaaaccagc atcatttcca gcctgtttct atgggaacag gcaaaatgca atcctcccca
    18481 gagctgcttc ctccatccct gtatctaacc cctttctgtt tctcctggct gatctggcat
    18541 tgaacttgga atggaggagg aggttctccc agccttgaaa agcctcaggt ggtgtaaggg
    18601 acttcccaag ggagggagat ttccttctgt cactaaccca tgccttctgc catttccatc
    18661 ttcctttggt gatctccatg actgagaagg aacactcagg gctttgaact atgcgcacat
    18721 tcattttctc caagtgagag gagaaaaatc ccctcagatg gggaccagca gctctgagct
    18781 ctgctcccag atctgttcct gtctccctga gtgatgggat gggaagactg ggttacagga
    18841 gataactggg agccctgtgc tgagatgggg tgaaccacgt tgcagggaat ttgatgatgc
    18901 tttttcactt gctcttctca aggtttaaaa ataaacatag gctgagtgtg gtggtgcaca
    18961 cctctaaccc cagcactgag gagggagggg cagggggaga tttatgagat tgaggccagc
    19021 ctggtacaca tagtgagttc caggccagcc tcagctgcat agtgagatcc tgcctcaaaa
    19081 atcataaata aataaataaa taaataaata aatagtgaaa gaaaaggcca ctggtggtca
    19141 gcaaagagta catggggatt tgcttgttcc caaagactgg aaaagtgaaa aagtgaactt
    19201 tggggcagtt ggctctgaac tgtgtcctgg ctctaaaggt ccagctgtgt gagccccaga
    19261 caccagcact gggtgatccc tgagaacagt gaagggcact ggcttgcaga acagggacct
    19321 tcctgttgag tttctgtgta gaggaaagga caaggaaagc acctggtgac tctgtggtat
    19381 gctccatgtg tggtggctca cacctctaat cccagcactc agagggctga agcagatggg
    19441 tctctgagtt taaggctagc ctggtctaca gaccacgttc taggctatct aggactgtac
    19501 agtagtgaga ccttgtcttt tagagaaatc tgtagtgagc accagagtgg caagctgttg
    19561 ataacactgt gaccattcac ttcctcactg tggtatttag tccctatcca tggggcaagg
    19621 aaataacatt tttcttctca tagaaattct ttagtttggg atttctccca gtcctgtggt
    19681 ctaggctttg acatctttaa gattagctgg gccaacttaa ccgcatgaca aattaattac
    19741 agaatagaga gctattttgt gacagaggta cctgataccg cactcactga aagttagcac
    19801 aaaaaaaaat cattgggaaa aacattgctc cctggtgagg tttctggatt tatggaatgg
    19861 agaggctcct ggtgaggaat ctatacaaat gccctgagac aagggtcact ctgagtgatc
    19921 tgggtaggag gagtttacat ctctctcctt acagatctta ccttgaactc taacatgtga
    19981 ccctacagag gaccgtttca ttctgtgggc cctgtgagtt ggttgagatc tctaaggatg
    20041 aaggactctc agctggtccc ctcctctgtt tgtccacaga gccagtgact cagcccttcc
    20101 tccaagtcac caacaccaca gtcaaagaac tagactctgt gaccctgacc tgcttgtcga
    20161 atgacattgg agccaacatc cagtggctct tcaatagcca gagtcttcag ctcacagaga
    20221 gaatgacact ctcccagaac aacagcatcc tcagaataga ccctattaag agggaagatg
    20281 ccggcgagta tcagtgtgaa atctcgaatc cagtcagcgt caggaggagc aactcaatca
    20341 agctggacat aatatgtgag tgaccttgcc acccttctta ttacagagtg ttgacggtgg
    20401 gtgcaattta tcaagggtgt gaaaaatggg taaaagtttc aaggagaaaa atacatctca
    20461 agatacaaat acagccacca tggcgatgga tgcgaatcaa ttcacagtaa aacacataat
    20521 aagctaagca tggcaccttt aatcccagca caccataggg atctctgagt tcccaaccag
    20581 ccttgtctac atagcaagtt ccaggttacc ctgcaaatac agactacaag tctttgagat
    20641 ggcgcaacgc aattaatgat aacttcgtat agcatacatt atacgaagtt atggtctgag
    20701 ctcgccatca gttcattgtt tttccactag tgttggagtt tcttgcacat tctgaatatt
    20761 aacctcttgt cagatgtttt acttacaaat atattctgct attctaaaag cggacttttt
    20821 tgtcactttc ctggtaaaag cctttgttta ttattagtaa tggtttttaa attggcacaa
    20881 aaattgaata catttaagtg aaattatatc tagatacagg aatgattaaa acaagttgat
    20941 tgacacagtt gttacttcct ttatactttt tatgctaaga catttaaaat ttattgttca
    21001 tttctttgca atacattatg gctgactata attcccctga tgtgtcagac ctgtcaaaac
    21061 ctaaccttct ggtctgtctt aaaccttgga ccctgaccag tgactcccca tcgctcctgc
    21121 tccacgaagt ctctggtcag cttcattcca ctctctcctt ccacattgtt gagatcatgg
    21181 aagagactgt gtggagtttc ccacgcaagg cctgtctcac ccagcatcct gtcctctagg
    21241 ctctttcaca gaatcacatg acagagctgc ctccttttaa aggtccagtg gcactcctat
    21301 gtgaagatac attcacattt tcttacttat tcatccactg atagacattt actgtccatg
    21361 ccttgattat tgtgaaaagt gccacaaaga cctgctgggc tgtatgactg ataagatcct
    21421 ctgatgcaca aacattttca attttgctaa agtccacttt atctattttt atcatggccc
    21481 tatgcatctg gcaccatgac agagaaactg agccaaattc aacctcaaga agcttccatc
    21541 ctgttttcta aaagttttat ggttggtgtt cggctctgat attagggtct ttgatacact
    21601 tagaattgat ttttatatga tgtaaggtga tggcttagaa acaatcactc ttttccctgg
    21661 atatcccatt tccccaatcc cttgtttaaa agataatcct tctatcattg agcaatctta
    21721 gcctgatgaa aactcttctg gtcccaggtg cagttggaca gcaagaatgc tttccaaatt
    21781 ctgtaactgg agaggacacc tctgctttac accaactaac tctttcaaaa gagccaggag
    21841 agatgaatgt tcataacacc aaaaaatgac aaatgaagtg agagatgtgc catcctctct
    21901 gatctgacag cacattatag tatacatgca aaacatatac agtggcccat aaacatgcac
    21961 atttatcatg ttcaactaat cttgaattga atcattggcc agtgtacatg agcagttctg
    22021 agctgtctat tctatttcac tactctatat gtctgctttt ctgtcagaca gttcggttat
    22081 tgtggatttg tactaaaatt attgttgtag aaaattttaa tcacaatctg gagtttctac
    22141 tccacctttg atcattcagt tctcagaaaa aagacacaca acttttatta tttacactaa
    22201 gccttaatct gtactagaac tggacagata tctaccctct atgctattaa aatctatttc
    22261 ctgtcaataa ccccaagata ttacttaata cgtttcatct gggctgatct taactccaat
    22321 tggccaggcc tcagggccat gttttcttga ctcctaaccc atggcgacat ttttttccct
    22381 taccatcatc ctccttcctc ctccccctca ttttcctact ccttccctcc ctccctccat
    22441 ccccacccca ccccaccccc cgttttccct ggatccctta gcaggaaccc taaaacccac
    22501 ctatgtctct tctgcccagc tattggctgt tggcatcttt atgtaccagt gagaaataac
    22561 tgtctggtct ctggggcaac catgtcttgg gggcccacac ttagcatttc aatacacagc
    22621 aaattggcta cagccaatta ctggagggat tagagggtgg actagaagta cctggttaga
    22681 gaaggagggg gagatggaca atgagccatc ccacggccag gagaaacagc tagtatctag
    22741 ggttcaccac tgtggaggta gacaggccag cagcagaata gattatgggt aatcctatag
    22801 taattgccaa agccaaataa aataaccata gcctgagtct tgtttatatg taagctagtc
    22861 ggggataggc ttaaattgat tgtactataa ttatgaaatc atgaaggatg tccatgcaat
    22921 tttcttcctc cttttcagga tacttttaag gttaggtgtg atggcacttc cctctaatcc
    22981 ctgatctcag gaggcagaga tagctggatc tctgagacat tgaggccagc ctagtctata
    23041 tagtaagctc caggacagct agaatagaca tttctatttc tgtataaaat atcactgggg
    23101 ttttaagcca atgtgcatag gactgacaga acaccatagt aatatcatca tctgtcttaa
    23161 aaagtctgtc agacagtctg gctcatgact gtgataccgg ccactcgaga agctgaggca
    23221 gaagtaccaa ggaagagtgc ctaacataac tagttcaagg ccaccctgag aaatttcgtg
    23281 gcacgctgtt tcaaaacaga cgttaaaagg cagagctcag gatatagctc agtggtagag
    23341 cattttttac tatgctcaa
//