LOCUS       258011#pL1L2GT1_LF2A_nEGFPOT2A_CreERT2_pAbActNeopA#L3L4_pD223_DTA_T_spec        20923 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2GT1_LF2A_nEGFPOT2A_CreERT2_pAbActNeopA
COMMENT     design_id: 258011
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000003345
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     misc_feature    4275..10182
                     /note="5 arm"
     misc_feature    10210..10234
                     /note="B1 Gateway"
     misc_feature    11201..11218
                     /note="FCHK QC critical region"
     misc_feature    11185..11200
                     /note="FCHK QC prefix region"
     misc_feature    11219..11224
                     /note="FCHK QC suffix region"
     misc_feature    11347..11398
                     /note="F2A"
     misc_feature    11224..11346
                     /note="1Dleader"
     primer          11070..11091
                     /vntifkey="27"
                     /dnas_title="seq primer FCHK2"
                     /label=seq primer FCHK2
                     /note="seq primer FCHK2"
     exon            11170..11223
                     /vntifkey="61"
                     /dnas_title="mouse En2 exon"
                     /label=mouse En2 exon
                     /note="mouse En2 exon"
     misc_feature    12270..12270
                     /note=" A to G to kill RsRII site"
     misc_feature    12220..12273
                     /note="self-cleaving' 2A peptide"
     misc_feature    12274..13299
                     /note="Cre"
     misc_feature    11404..11493
                     /note="3X NLS"
     misc_feature    11494..12210
                     /note="EGFP"
     primer          complement(10363..10384)
                     /vntifkey="27"
                     /dnas_title="LRPCR LAR3_EN2"
                     /label=LRPCR LAR3_EN2
                     /note="LRPCR LAR3_EN2"
     intron          10295..11169
                     /vntifkey="21"
                     /dnas_title="mouse En2 intron"
                     /label=mouse En2 intron
                     /note="mouse En2 intron"
     primer          complement(10259..10282)
                     /vntifkey="27"
                     /dnas_title="FRT3_oligo"
                     /label=FRT3_oligo
                     /note="FRT3"
     misc_feature    10247..10294
                     /vntifkey="21"
                     /dnas_title="Frt"
                     /label=Frt
                     /note="Frt"
     misc_feature    14560..14567
                     /vntifkey="21"
                     /dnas_title="polylinker"
                     /label=polylinker
     misc_recomb     14528..14559
                     /note="Rox(inv)"
     misc_feature    14520..14527
                     /vntifkey="21"
                     /dnas_title="RE sites"
                     /label=RE sites
     primer          14460..14478
                     /vntifkey="27"
                     /dnas_title="seq primer NF"
                     /label=seq primer NF
     primer          14334..14357
                     /vntifkey="27"
                     /dnas_title="LRPCR RAF5"
                     /label=LRPCR RAF5
     misc_feature    14277..14519
                     /vntifkey="21"
                     /dnas_title="SV40 pA"
                     /label=SV40 pA
     misc_feature    13309..14253
                     /note="ERT2"
     misc_feature    15767..15767
                     /note="G to C conservative mutation to kill RsrII site"
     misc_feature    16115..16358
                     /note="SV40pA"
     misc_feature    16173..16196
                     /note="RAF5"
     misc_feature    15922..15941
                     /note="neoBstB1_5"
     misc_feature    15629..15647
                     /note="neoSphI_5"
     misc_feature    15292..15311
                     /note="neoPstI_5"
     misc_feature    15123..16108
                     /note="neomycin resistance "
     misc_feature    14973..14992
                     /note="betact5"
     promoter        14577..15097
                     /note="human beta actin promoter"
     insert          14274..16353
                     /source="pL1L2_GTIRES_BetactP FLFL MapVerified"
     misc_feature    16354..16358
                     /note="for bActNeo"
     misc_recomb     16360..16391
                     /note="Rox"
     misc_feature    16404..16424
                     /note="B2 Gateway"
     misc_feature    10183..16454
                     /note="Synthetic Cassette"
     exon            16805..16899
                     /db_xref="ENSEMBL:ENSMUSE00000323312"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 1 ENSMUSE00000323312"
     misc_feature    16455..17050
                     /note="Critical Region"
     misc_feature    17074..17107
                     /note="Downstream LoxP"
     misc_feature    17051..17130
                     /note="synthetic loxP region"
     exon            19173..19268
                     /db_xref="ENSEMBL:ENSMUSE00000116783"
                     /rank="5"
                     /note="ENSMUSE00000116783"
     misc_feature    17131..20923
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggatgtgct agattaattc ctagatggga actgctaggc ctctgcattg
     4321 gtttctgatc tttgtcatgc aagctaagac actagtggaa gaagaagaga ggagaatggg
     4381 tgtgctcaga taagctactc tgctcagcta agctgctctc cattgatcac cagtccttct
     4441 ctaagacaga gccaagagca tcagcagatt agacctcaag atgtgcggag gtaggatgaa
     4501 acatcttata aagagtgtta gggaaactct ccaaacattg aaggcccaat tttacatttc
     4561 accacatcct aattcaaata aacagttatt cacatgcaaa aagtttcaaa actagattaa
     4621 agcacaaagt atttattttt gggtgaatga ggtattaaca ggatacccca aagcatataa
     4681 gccaagcaca agagatttga ttatataaat gtgaatttaa aattaccata attttgacaa
     4741 attattcaat gcaattgaac taagatataa aggatttaga cagtaaacta accagaaaaa
     4801 ggtttatatt ttatttttta atgaacaaaa tgtttgagaa aaaatagcta gagaaaaatt
     4861 aaacagggga agctgaggca gaaagatcat aagttcaaag ctaccctgat caacttagca
     4921 aaagtgtttc aaaatgaaaa atgtaaacag gggttaaggt gatatagttt tggggtgggc
     4981 catccattgc taggtccagt cctaagcact gcaaaatcag aaagtaacaa ggaacttctg
     5041 agggctgtga catgaaaatg cctatcttca ggactcacca aagaatggtg aagatgacaa
     5101 tgactcaggg gtcatcccgg cccatgttca atgacaggag aaagttttct gttaagacaa
     5161 cttttctgta aagcaattcc gaagaagctt tgaaatcagt ggtttttacc ttttgacaca
     5221 gcactttact cctaagatga tattctaata gttcagagta agtgtggaaa actgaatata
     5281 aagatatcaa taaataatga aatactgata atgttaatcc taaaaagtta attgtaatga
     5341 ttagtaaaat taatttttaa gctgaaatat atgcaagatc tttacttgac agctctgtga
     5401 ggcagatact tctactgtct gcattttaaa gatattttat tcatgtattt gtaatcatgt
     5461 gtttgcctat gtttctctgt gggagtgtat acacatgaat ggtgatgttc ctgaggcgag
     5521 aggcatcaca gccttttgaa gctggaggta cattgaggtg gtcgcaaatc acccaaagta
     5581 gctgctactc tctgggaacc aaacttgggt cctctgcaca agtagtgtga gttcttaact
     5641 actgagccat ctacccagcc ctatatatac tatatatttt gtcaatgaca taaaagaaaa
     5701 cacctaagtt tagctagtcc atgtaccatg ctcagtaaac tatatatata gtccctgtcc
     5761 atatatataa atggaccctg tccatttttg tgtttcaaac ccttttgttt tatctacttt
     5821 ctgacatggc tcctaaattc atgagtaatg gtgaacatca gttttactga tgctcatctc
     5881 tgcaaaactt ttacttaaag ttgtgtgtgt atgtgtgtgt atgtgtgtgt gtgtgtgtgt
     5941 gtgtgtgtgt gtgtgtgtat cagatgaaaa cattctggag ttagttctct ccttccatca
     6001 catgggttca agtgatcagg tcactaggtt ggtggcaaga gtttttacca gctgacccat
     6061 ctctcaggcc cttctcctct tatttcttaa ataagacttt ctgtgtgctg gactccgtgc
     6121 taagcacttt acacatcttt cttgtttaca ctcttccaca caaatatatg ataaatgcca
     6181 taaactatgt ttcattttct aaaaaataat tcccagtgtt taaccactaa cgcctttaaa
     6241 atttaagtac tatttgtaac aattatatta aaatgtaaat ctctctctct ctttctctct
     6301 ctctctctct ttctctctct ctctctctct gtgcgtgcct gtgtgtgtgc atgtataatt
     6361 gatttaagct atgaacacag gaagtcctcc ttggttttct gctgattgga tggtattagc
     6421 tagctatgtt ccacaaacag tgttctgtgt tgaagctact ttggccaaag atggttaaca
     6481 tgaatgttta agcagatgcc tggtgatctt cccatagtgc ttctcagagc tgcctatgat
     6541 actgtgtcct ggagagatgc tgtggtgtag gcaggatatc ccaggcggct tctcttctca
     6601 gtgttaggaa gaataagtaa tgctcattgg aacacatttt ggattgggaa acacacttaa
     6661 acacaaggtg catgcatgta ttacaaaggt cacaattagc ctttgtgaaa gcatttcact
     6721 tgagggccca tttctgtttt agcagagaaa gctggctaat tgataggact gccatgcaca
     6781 caacctcaga gcagcatttc agcagcagtc caaagaaatg aggtcagccc cagaggctcc
     6841 tgggagctga ggaaattttg ggagaagctt tgagccactg tctgtattcc caaacacagc
     6901 tcgtgcccta accctggctc tttctgctgt ctctcctgtg tcattcactc aacctcaggt
     6961 atagaagctg tgttgtagat gtgccagctg ggaattgttt tcttgtggcc atttattctc
     7021 tgcattttga ccatctttgg atctctgcag cagcctctgt ccattgcata gaataagctt
     7081 ctttgatgag tgataactca ctgtgggtat aaggataaga ctttagaaca tagttagaaa
     7141 cctattgatt tagaaacttg acagttttag ggtttccatc ctatagggtc cattactcct
     7201 ccagccactg gtagcttgat aagtttatag tcctaagcat gaatttcttc ccattgagca
     7261 gccttaagtc caattaaata gcagttggta gcctctaagg taaaagtgct actattgttc
     7321 cgctggggat atcttatctt gcttgtcatt actaggattt gtaagcttta tatttcgaga
     7381 gggctattga ttgcttttct cccttggctg cttgaatggc atttcaaata ctgtaagagc
     7441 tagttagtcc tcagggagga ggctcccagg tcagctctaa cttgattcct ctgagtccag
     7501 tgtcgggagt gtgtggtgtg tcttcagaaa tagaatttta ccttcaagtt ctgggaggca
     7561 cccaatgaaa atggcagcag cctatatatt gttttaggag ttctgaacac cctgaccaac
     7621 aatacgaagg gaggtttctt tgcctgggtc tggggtttct gttatgtagt ctgtggctct
     7681 tgcagggagg actgtttcca atgtagtgta actccagtga caatattcta caggcttatt
     7741 ggagtggttg cttattgttc tatataagct atattatttt taactaaaac atctacatca
     7801 aggctatgca agcaaagaat actcatattg gagtgcaata tctaccattc ttcttagagg
     7861 ataaaaataa gctcatgaag ttggcttcaa atggaaatct attttactaa atactttgga
     7921 aaaagcccaa cattttgaat agaggctcct atctaatata aactgagtat tcttcctata
     7981 tatggtactg tgtagtttat atacaagctt ataaactact aatcttatat catgtattag
     8041 atattaactg ccaacagtag ttttcagcat gaattcagca ttaattactt tgtagggaaa
     8101 ctggacttta catgtttgct cttcttcttt gtttttgttt ttgtttttgt ttttgttttt
     8161 gtttttcgag acagggtttc tctgtgtaac cctggctgtc ctggaactca ctctgtagac
     8221 caggctggcc tcgaacgcag aaatccgcct gcctcagcct cccaagtgct gggattaaag
     8281 acatgcgcca tcacgcctgg cttatttgct cttttatatt atattctgtt atcatagtaa
     8341 gttctttgtt ttagtttggt tttgtgcttt tgggtaattt ttaaagaata gaaacggaat
     8401 tggctcatct ggagatgcaa agtcccacat ctggaaagtt tctccaccac accacatcat
     8461 aaccatttcc tgagccaagg agagagaagt gaagaactca gtctttagaa gactctgccc
     8521 tcttgatgag cctgcttgct caatcgtcgc attaatccat tcttgagggc attctgacag
     8581 tctctgcttg aagacttcac ttcctaacac tgccgtactg agaattacat ttctaaaaca
     8641 taaactttga gagacacatt taagtggaaa tatttctcag ctgttaaaat ttcccaaaat
     8701 cgttgtgcac tttaaaagga aggctcgccc ttcttcactt gggtctcaac ccacctgtta
     8761 cctatggcat cagcttcttg gaagccattt ctatgttaag atggcttctt tccaaattct
     8821 ctgtgtatac accctgtaat ggacagtcac taagagaaac atgactgtaa tattgcggtt
     8881 tttagcatta atgagtaaag ttttacataa ttttgtgtga ggccagtttc atttttcact
     8941 gcaaagatca tttgatggac agtggcatac cccaacttgt ctagaacctg ttttcttagc
     9001 tatgtataat tttaatcaga cttagacaga gacttaatga aaatgccagg agcccttgtt
     9061 gtcgggatta tagaaataca tagtatttgt cacaaaatgg aggagtatat ttcttggtgc
     9121 aagtttgcat gtagccaggt cagggtgaat atgatatatg agatggtgta gttttcacat
     9181 cattggtggc tttatcatga gagaatatat cttcacttgc ttgaatacag gggtggatat
     9241 gggttctgtg tcacctttgt cttgtccata cttgtatgcc tctgcttcct ccaactccac
     9301 aggggcttga atccttgctg actctctctg ccttctctcc agcatatcca tgcaagtctc
     9361 ttcagacaca tggggttcaa acctcattat caagggcatc ttgagcctgc ttagtagtgc
     9421 actcttcctt acaggcttcc tcacttccta tgttgtgagg tacacccaac ctcaggggtg
     9481 tgaggaccgt tgattatgta gacttgccag ggagataggg aaaacctaaa gttccatgtc
     9541 agttacatgc tggctttcac acactcttca aggacgaatt ctatcatctc agacctcttt
     9601 tatgttgtcc atttaggctc cttgctgaac ccagaactgt tcactgtctg cccaaagatc
     9661 atgtgactgt tttcagttta caaatgattt caatctctgc ttcactgcct tagcctctat
     9721 caacctccta gtgataaagg cttagacatt aatgtgacca ggtggggcct tccggctact
     9781 gtttgcctat cattctggta cattcacaag cccttccatg tagacccagc atttgcatct
     9841 tatcctctat gctttgctga gtctgaccat gtcaggagga agaaagtgga tagcgtcggc
     9901 aaagccccag tggtgcttta tacctctgct gctcaaagaa gcttcgcatc atgacaatta
     9961 tgaataagcg tgtctgccac ctaccaatgt gcaaggaaaa tttagaccca taggctgatg
    10021 aagccaaagc tttctggaag gtcttattca aaactgtatt taattttgag aaataaatac
    10081 attttacttg tcaccttaag aagaaagttc cttttgaatt ataaccactt tcaacaattt
    10141 tactagttaa atcactaagc cacttatatc tgatgcatac aaaaggcgca taacgatacc
    10201 acgatatcaa caagtttgta caaaaaagca ggctggcgcc ggaaccgaag ttcctattcc
    10261 gaagttccta ttctctagaa agtataggaa cttcgaaccc tttcccacac caccctccac
    10321 acttgcccca aacactgcca actatgtagg aggaaggggt tgggactaac agaagaaccc
    10381 gttgtgggga agctgttggg agggtcactt tatgttcttg cccaaggtca gttgggtggc
    10441 ctgcttctga tgaggtggtc ccaaggtctg gggtagaagg tgagagggac aggccaccaa
    10501 ggtcagcccc ccccccctat cccataggag ccaggtccct ctcctggaca ggaagactga
    10561 aggggagatg ccagagactc agtgaagcct ggggtaccct attggagtcc ttcaaggaaa
    10621 caaacttggc ctcaccaggc ctcagccttg gctcctcctg ggaactctac tgcccttggg
    10681 atccccttgt agttgtgggt tacataggaa gggggacggg attccccttg actggctagc
    10741 ctactctttt cttcagtctt ctccatctcc tctcacctgt ctctcgaccc tttccctagg
    10801 atagacttgg aaaaagataa ggggagaaaa caaatgcaaa cgaggccaga aagattttgg
    10861 ctgggcattc cttccgctag cttttattgg gatcccctag tttgtgatag gccttttagc
    10921 tacatctgcc aatccatctc attttcacac acacacacac cactttcctt ctggtcagtg
    10981 ggcacatgtc cagcctcaag tttatatcac cacccccaat gcccaacact tgtatggcct
    11041 tgggcgggtc atcccccccc ccacccccag tatctgcaac ctcaagctag cttgggtgcg
    11101 ttggttgtgg ataagtagct agactccagc aaccagtaac ctctgccctt tctcctccat
    11161 gacaaccagg ctcccaggtc ccgaaaacca aagaagaaga acgcagatcg cagatcgcag
    11221 atctctagag tcaccgagtt gctttaccgg atgaagaggg ccgaaacata ctgtccaagg
    11281 cccttgctgg caatccaccc aactgaagcc agacacaaac agaaaattgt ggcaccggtg
    11341 aaacagactt tgaattttga ccttctcaag ttggcgggag acgtcgagtc caaccccgga
    11401 ccggtgcatg tggaccccaa gaaaaagcgg aaggtggacc ctaaaaagaa acgcaaagtg
    11461 gatcctaaga aaaaaagaaa ggtgcacgtg tccatggtgt ccaagggcga ggaactgttc
    11521 accggcgtgg tgcccatcct ggtggaactg gatggcgacg tgaacggcca caagttcagc
    11581 gtgtccggcg agggcgaagg cgacgccaca tacggaaagc tgaccctgaa gttcatctgc
    11641 accaccggca agctgcccgt gccttggcct accctcgtga ccacactgac ctacggcgtg
    11701 cagtgcttca gcagataccc cgaccatatg aagcagcacg acttcttcaa gagcgccatg
    11761 cccgagggct acgtgcagga aagaaccatc ttctttaagg acgacggcaa ctacaagacc
    11821 agggccgaag tgaagttcga gggcgacacc ctcgtgaaca gaatcgagct gaagggcatc
    11881 gacttcaaag aggacggcaa catcctgggc cacaagctgg agtacaacta caacagccac
    11941 aacgtgtaca tcatggccga caagcagaaa aacggcatca aagtgaactt caagatccgg
    12001 cacaacatcg aggacggctc cgtgcagctg gccgaccact accagcagaa cacccctatc
    12061 ggcgacggcc ctgtgctgct gcctgacaac cactacctga gcacccagag cgccctgagc
    12121 aaggacccca acgagaagag ggaccacatg gtgctgctgg aattcgtgac cgccgctggc
    12181 atcaccctgg gcatggacga gctgtacaag cctgcaggcg agggcagagg aagtcttcta
    12241 acatgcggtg acgtggagga gaatcccggg ccgtccaatt tactgaccgt acaccaaaat
    12301 ttgcctgcat taccggtcga tgcaacgagt gatgaggttc gcaagaacct gatggacatg
    12361 ttcagggatc gccaggcgtt ttctgagcat acctggaaaa tgcttctgtc cgtttgccgg
    12421 tcgtgggcgg catggtgcaa gttgaataac cggaaatggt ttcccgcaga acctgaagat
    12481 gttcgcgatt atcttctata tcttcaggcg cgcggtctgg cagtaaaaac tatccagcaa
    12541 catttgggcc agctaaacat gcttcatcgt cggtctgggc tgccacgacc aagtgacagc
    12601 aatgctgttt cactggttat gcggcggatc cgaaaagaaa acgttgatgc cggtgaacgt
    12661 gcaaaacagg ctctagcgtt cgaacgcact gatttcgacc aggttcgttc actcatggaa
    12721 aatagcgacc gctgccagga tataaggaat ctggcatttc tggggattgc ttataacacc
    12781 ctgttacgta tagccgaaat tgccaggatc agggttaaag atatctcacg tactgacggt
    12841 gggagaatgt taatccatat tggcagaacg aaaacgctgg ttagcaccgc aggtgtagag
    12901 aaggcactta gcctgggggt aactaaactg gtcgagcgat ggatttccgt ctctggtgta
    12961 gctgatgatc cgaataacta cctgttttgc cgggtcagaa aaaatggtgt tgccgcgcca
    13021 tctgccacca gccagctatc aactcgcgcc ctggaaggga tttttgaagc aactcatcga
    13081 ttgatttacg gcgctaagga tgactctggt cagagatacc tggcctggtc tggacacagt
    13141 gcccgtgtcg gagccgcgcg agatatggcc cgcgctggag tttcaatacc ggagatcatg
    13201 caagctggtg gctggaccaa tgtaaatatt gtcatgaact atatccgtaa cctggatagt
    13261 gaaacagggg caatggtgcg cctgctggaa gatggcgatc tcgagccatc tgctggagac
    13321 atgagagctg ccaacctttg gccaagcccg ctcatgatca aacgctctaa gaagaacagc
    13381 ctggccttgt ccctgacggc cgaccagatg gtcagtgcct tgttggatgc tgagcccccc
    13441 atactctatt ccgagtatga tcctaccaga cccttcagtg aagcttcgat gatgggctta
    13501 ctgaccaacc tggcagacag ggagctggtt cacatgatca actgggcgaa gagggtgcca
    13561 ggctttgtgg atttgaccct ccatgatcag gtccaccttc tagaatgtgc ctggctagag
    13621 atcctgatga ttggtctcgt ctggcgctcc atggagcacc cagtgaagct actgtttgct
    13681 cctaacttgc tcttggacag gaaccaggga aaatgtgtag agggcatggt ggagatcttc
    13741 gacatgctgc tggctacatc atctcggttc cgcatgatga atctgcaggg agaggagttt
    13801 gtgtgcctca aatctattat tttgcttaat tctggagtgt acacatttct gtccagcacc
    13861 ctgaagtctc tggaagagaa ggaccatatc caccgagtcc tggacaagat cacagacact
    13921 ttgatccacc tgatggccaa ggcaggcctg accctgcagc agcagcacca gcggctggcc
    13981 cagctcctcc tcatcctctc ccacatcagg cacatgagta acaaaggcat ggagcatctg
    14041 tacagcatga agtgcaagaa cgtggtgccc ctctatgacc tgctgctgga ggcggcggac
    14101 gcccaccgcc tacatgcgcc cactagccgt ggaggggcat ccgtggagga gacggaccaa
    14161 agccacttgg ccactgcggg ctctacttca tcgcattcct tgcaaaagta ttacatcacg
    14221 ggggaggcag agggtttccc tgccacagct tgatgaagat ctgagctctt aattaatgat
    14281 cataatcagc catatcacat ctgtagaggt tttacttgct ttaaaaaacc tcccacacct
    14341 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
    14401 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
    14461 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatccg
    14521 tttaaactaa ctttaaataa ttggcattat ttaaagttac atatggcggc caattcgcct
    14581 tctgcaggag cgtacagaac ccagggccct ggcacccgtg cagaccctgg cccaccccac
    14641 ctgggcgctc agtgcccaag agatgtccac acctaggatg tcccgcggtg ggtggggggc
    14701 ccgagagacg ggcaggccgg gggcaggcct ggccatgcgg ggccgaaccg ggcactgccc
    14761 agcgtgggcg cgggggccac ggcgcgcgcc cccagccccc gggcccagca ccccaaggcg
    14821 gccaacgcca aaactctccc tcctcctctt cctcaatctc gctctcgctc tttttttttt
    14881 tcgcaaaagg aggggagagg gggtaaaaaa atgctgcact gtgcggcgaa gccggtgagt
    14941 gagcggcgcg gggccaatca gcgtgcgccg ttccgaaagt tgccttttat ggctcgagcg
    15001 gccgcggcgg cgccctataa aacccagcgg cgcgacgcgc caccaccgcc gagaccgcgt
    15061 ccgccccgcg agcacagagc ctcgcctttg ccgatcctct agagtcgaga tccgccgcca
    15121 ccatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
    15181 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
    15241 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
    15301 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
    15361 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
    15421 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
    15481 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
    15541 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
    15601 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
    15661 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
    15721 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggccgac cgctatcagg
    15781 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
    15841 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
    15901 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
    15961 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
    16021 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
    16081 cgcccacccc ccggatctaa gctctagata agtaatgatc ataatcagcc atatcacatc
    16141 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
    16201 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
    16261 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
    16321 gtccaaactc atcaatgtat cttatcatgt ctggatccct aactttaaat aatgccaatt
    16381 atttaaagtt aggaattaat tcacccagct ttcttgtaca aagtggttga tatctctata
    16441 gtcgcagtag gcggtttatt tctgtacagt gatccgggat taattttgct tttagaattt
    16501 tgtagctttg tctactattt ttgtgttcta aagatttgct aggttccctt tagcaggcga
    16561 ctagagcaca ctgttgttgc agttttcaga cgaatgacac tgttaacgtt tcctgagacc
    16621 cagtggtttg ctgccatcct gtaagtaact gaggaggtaa agcatgagag cgaatgtaag
    16681 gctcggagtc tctcaaggaa agatgtagct gtgccttcac agttgtttct gtgccactta
    16741 gcaaagcagc ggagaaaaaa gcaatgcctc cttattccat gcagctgttt ttctccaatg
    16801 ccaggtatgc aggaggcctc aatgaagctc actgaatctc tacatgaagt ctacgagcca
    16861 gactggtatg gacgggaaga tgtaaagatg gttggtgagg taagagctgg atggcaactt
    16921 ctaggagacc ccgagtggct tccagaagtc ttcgagtgct ccgtccacat tccacttctc
    16981 cacctcagag catgaatcgc aaaccaagat gagatactga aaatgcagcc tagagttcta
    17041 gtacctaagc gagatggcgc aacgcaatta atgataactt cgtatagcat acattatacg
    17101 aagttatggt ctgagctcgc catcagttca actctgaaac catttgaact ttgggttaaa
    17161 gattgctttt cttaaaaata accatagctc ttttctatta catactagag gttgtaacag
    17221 ctatttgtag ctgagccttt tatcaacttg atttccatgc agtgattcca ttcccttttg
    17281 ccccttctcc cttttaatga gcccgcctgg aacgcagaag caaaatctca gacgctcgcc
    17341 catccctctg tagcacacct agtccagtca ctaaactcaa ctgaacttta gtgcagcctt
    17401 ggacttagaa ataccctcac acctgagtta aagactacag ctctaggtca ctcagccctc
    17461 aggacatgag cacatgagca tcaccacttt gtgaaaatgg agcagaacag tcagcaagaa
    17521 tggccacgtc acctcaggca gctcaagatg gaaataatgc caattaaact aacagaaatc
    17581 acatttgaag cctttgtgtg aaggtcctct catcgtgctc agaacatgtc cttgtcactg
    17641 atgggagaga gcttctaggc cgcagttatc tcccctccaa gatctttggt gaaaagaaga
    17701 gcagttgttt gtcatgtcgt catgtcaggt gggtcaatac agctcctcta gaaacagaag
    17761 atccgggtta gatgatggag ccaaagccca agggaaggtt aattcaggag cttctgcagg
    17821 ggttcacagt gagtctctgg ctgctgagtg gcagcgctga taccccgatg ttgatcttca
    17881 tagtatcatt agaactgaaa tgaaaatggg ttcatctatg ggagtggttc ttgttagata
    17941 tattctatct tccaaaatct ttcaggatgt gaatttggca aagctgttcc cacataccac
    18001 ttttaaatgc agttaccaag tgtttttgtc atcttacatt ttccctatat gaaaacatca
    18061 tagttcaaca acatgtttta aaatgcttat ctgtctttgt acatttctga ctaataaagt
    18121 atcctcaaaa ctcatcagaa ggaaggaaat tttaaagaat aatggaaagc tgtgttccca
    18181 tcctcagacc attgtatgat tacatttcgt ccaccacaat gctactgttg acattggttc
    18241 tttaatgaaa tggctgctat gctacactag cccctgtgta ttccaggaca tttgaaccct
    18301 cggctccagt ttcaaacatc atcctttgga caataaagcg atcattatat gttcaaagtg
    18361 tctacttaca acagatgatg agcctttttt aattgatgaa tttaataatt ttgtttgggc
    18421 attataacta ggatttagtt tttgaaggtc aaaacaggaa acaaaatgca aataaaactt
    18481 ctttttcttg taaatgatat caggtagcct aaactgggct caaacccact atccagctaa
    18541 ggctggcctt gatcttcttg gctctatttc ccaagtgcag acattagagt tgtgggccag
    18601 tgtctccacc tttagattag ttttgtattg ttttaatgtt taaacaagat caagatttac
    18661 cattcactta aagaaatttg gaagtaacag cccgagtaag atatgatgaa ctgacaagta
    18721 ctggagacat tttcttttta attcactgta tacctcatgg gcgataaggt ataatacatt
    18781 ttctcctcat tgtgtacatg atgtttgtct ctctctgggt tggctgtaat ttcgtcaact
    18841 gctgcagata agttagctgt ctcataggaa ttctcaatct tttactccag tggccattta
    18901 ttgtaacttg ggaatctgtt aacccctctg cattcctcag tatcgcagaa tcagcgacgt
    18961 actttaagca agccaagctc ctggcttgtg ttgttaacta gaacactgac cacattgcct
    19021 tgacgtttga ttgcatccta attaggtcat agtttatatt gcctcttcta atatctcagg
    19081 aaggatggct tactatgtgt ttcgatttta gatgttttag cagtttggtg aaatgtgtct
    19141 gggcttatgg agtctctctg tttctctttt agaagtgtga tgtgctgtgg gaagacttcc
    19201 atcaaaagct agtggatggc tccttgctga cactggacac ctacctgggc cagtttcctg
    19261 acataaaggt atttcattct ccacgtgtga aggaaataat tgaatggtct catgcagtta
    19321 ttaaaatcag ggcaacagag gggaattaaa agttctcaag ctgaaatgaa agacatttgt
    19381 aacttttcat ttatttaagc atgtatctta aatctattat cgtgaaataa aattcagcta
    19441 cagaaatcaa agtggcctct tagtgaacgc ttcaatgctt ctttctttac agaactcttt
    19501 tcaaacctgt agatacgaaa tcagaactca aatacctaaa atctattcca acatttttat
    19561 aagtagtatg aggtgatttt cattaaaata aatgatggtc acagggagtg gtgaaactga
    19621 actgtaaatg caaggagaaa tctgatattt cactgtttac tgttagtttc acaggcagtc
    19681 acagtgcacc tgtgcatttt gctgccctta aattttcaga ttaaaattga aatatgagcc
    19741 ttttaagatg tgaaaaaatt atgtttaaaa caatagcatt ggctcttaac gcccctgcca
    19801 gggtaaataa agaagtgaat ggttgaatgt cgtagatctg gaactgccat tgtagctaaa
    19861 gctgggtctg aaaaaacatc tgtatgcaac agatgtgcag cactagcccc tttgctctgt
    19921 gctcaaatgg agtcagttgg ccttctagca ggttgaggag gttatgaacc agcaccagtg
    19981 gacatctggg gcatctcgtt tcacaaatcc actgtggcct ggccttctgt gtagtcaaga
    20041 acaaatacca ggaagcctcc tatagggaat gcactggctt tggctacaca gtgaaatgaa
    20101 atggcgtcac cacatcacac tcagagggag gacgagtttt tctgctccca cagactatga
    20161 ataaagagcc tttggtgact tccaggcaag aacgtaagtg gatctctcca agtatattct
    20221 tcttggtggc cacatgtcaa caaaacaaat ctgaatgatg gagtccctgt gttaacattc
    20281 tcactagaga tttcttcgct gtgtcctcat tttcctcgcg tctctgtgta ggcatctcta
    20341 cattagacag aacagccacc tgactattag caaacattaa agtctagaag gcaactacga
    20401 gagttcacta gatcctcacg attaagaatg aggagtttga tgagggactc aggattgtga
    20461 tagatgcaga ctgtgtattg ataaacagag tctcagtagt ggtgctttca gacaggctgg
    20521 gaacttaact gtgcacagta atgtgcctgt ttattctaat gtgcttgctc tatttcgtgt
    20581 cctccttggt tctggagagt ttcttacccc cactgtcact ggtttctgat gcgctcctct
    20641 tgatggtctc ctcttggact gatcctaata gtcatcccag ttacattttg agaaacctgt
    20701 cagtctttct gtagtacaac ccacctcctg tttttttttt taaataaact ttattttaaa
    20761 aaaatccttg ctaatgaata agaatactac gcgtcaaagt taacaagacc caaagtctat
    20821 acagcttagt gaacttctct acactgaacg tcggccttcg acatggaagc tatgaaggtt
    20881 cgcatcagat tccgtgctgt tttgattgaa tacattttgt gtg
//