LOCUS       46465#pL1L2GT0_LF2A_nEGFPOT2A_CreERT2_pAbActNeopA#L3L4_pD223_DTA_T_spec        21195 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2GT0_LF2A_nEGFPOT2A_CreERT2_pAbActNeopA
COMMENT     design_id: 46465
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000026499
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            8444..8560
                     /db_xref="ENSEMBL:ENSMUSE00000969850"
                     /rank="3"
                     /note="ENSMUSE00000969850"
     exon            5143..5216
                     /db_xref="ENSEMBL:ENSMUSE00001093883"
                     /rank="2"
                     /note="ENSMUSE00001093883"
     misc_feature    4275..9115
                     /note="5 arm"
     misc_feature    9143..9167
                     /note="B1 Gateway"
     misc_feature    10134..10137
                     /note="FCHK QC critical region"
     misc_feature    10118..10133
                     /note="FCHK QC prefix region"
     misc_feature    10138..10143
                     /note="FCHK QC suffix region"
     misc_feature    10266..10317
                     /note="F2A"
     misc_feature    10143..10265
                     /note="1Dleader"
     primer          10003..10024
                     /vntifkey="27"
                     /dnas_title="seq primer FCHK2"
                     /label=seq primer FCHK2
                     /note="seq primer FCHK2"
     exon            10103..10142
                     /vntifkey="61"
                     /dnas_title="mouse En2 exon"
                     /label=mouse En2 exon
                     /note="mouse En2 exon"
     misc_feature    11189..11189
                     /note=" A to G to kill RsRII site"
     misc_feature    11139..11192
                     /note="self-cleaving' 2A peptide"
     misc_feature    11193..12218
                     /note="Cre"
     misc_feature    10323..10412
                     /note="3X NLS"
     misc_feature    10413..11129
                     /note="EGFP"
     primer          complement(9296..9317)
                     /vntifkey="27"
                     /dnas_title="LRPCR LAR3_EN2"
                     /label=LRPCR LAR3_EN2
                     /note="LRPCR LAR3_EN2"
     intron          9228..10102
                     /vntifkey="21"
                     /dnas_title="mouse En2 intron"
                     /label=mouse En2 intron
                     /note="mouse En2 intron"
     primer          complement(9192..9215)
                     /vntifkey="27"
                     /dnas_title="FRT3_oligo"
                     /label=FRT3_oligo
                     /note="FRT3"
     misc_feature    9180..9227
                     /vntifkey="21"
                     /dnas_title="Frt"
                     /label=Frt
                     /note="Frt"
     misc_feature    13479..13486
                     /vntifkey="21"
                     /dnas_title="polylinker"
                     /label=polylinker
     misc_recomb     13447..13478
                     /note="Rox(inv)"
     misc_feature    13439..13446
                     /vntifkey="21"
                     /dnas_title="RE sites"
                     /label=RE sites
     primer          13379..13397
                     /vntifkey="27"
                     /dnas_title="seq primer NF"
                     /label=seq primer NF
     primer          13253..13276
                     /vntifkey="27"
                     /dnas_title="LRPCR RAF5"
                     /label=LRPCR RAF5
     misc_feature    13196..13438
                     /vntifkey="21"
                     /dnas_title="SV40 pA"
                     /label=SV40 pA
     misc_feature    12228..13172
                     /note="ERT2"
     misc_feature    14274..14856
                     /note="synthetic fragment mutates RsRII"
     misc_feature    14686..14686
                     /note="G to C conservative mutation to kill RsrII site"
     misc_feature    15034..15277
                     /note="SV40pA"
     misc_feature    15092..15115
                     /note="RAF5"
     misc_feature    14841..14860
                     /note="neoBstB1_5"
     misc_feature    14548..14566
                     /note="neoSphI_5"
     misc_feature    14211..14230
                     /note="neoPstI_5"
     misc_feature    14042..15027
                     /note="neomycin resistance "
     misc_feature    13892..13911
                     /note="betact5"
     promoter        13496..14016
                     /note="human beta actin promoter"
     insert          13193..15272
                     /source="pL1L2_GTIRES_BetactP FLFL MapVerified"
     misc_feature    15273..15277
                     /note="for bActNeo"
     misc_recomb     15279..15310
                     /note="Rox"
     misc_feature    15323..15343
                     /note="B2 Gateway"
     misc_feature    9116..15373
                     /note="Synthetic Cassette"
     exon            15749..15836
                     /db_xref="ENSEMBL:ENSMUSE00001008319"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 1 ENSMUSE00001008319"
     misc_feature    15374..16004
                     /note="Critical Region"
     misc_feature    16028..16061
                     /note="Downstream LoxP"
     misc_feature    16005..16084
                     /note="synthetic loxP region"
     exon            17818..17963
                     /db_xref="ENSEMBL:ENSMUSE00001008099"
                     /rank="5"
                     /note="ENSMUSE00001008099"
     misc_feature    16085..21195
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggaattata ccatgattaa gaggatgtgt tcagaattct tttttttttt
     4321 tcaaaaggaa gagtaaaaac ttttagtgaa taaataacaa acaaataaac atcatatcct
     4381 caatagagaa cattcttggt agttttatgt caacttgata cagccagtgt tatttgagtg
     4441 gaggataccc cagttgagag aacactttta aatgatcagg ctgtatgcag gcaaattttt
     4501 aggaaaattt ctcaattact agttgaggaa gtaggcctca ttccattttg tgtagtgcca
     4561 cttctaggct ggtggcccaa agctctataa gaaagcaagc taagcaaata tttgggaaca
     4621 agtgagtaac aagacatgtc catggtgtct tcatcaaacc tgcctccagc ttccttctgt
     4681 gtttgaggtc ccactttggc ttctctcagt gagatgtgct ttgggacata taaggcatat
     4741 aaaccttctc caagttgttt gtggttatgc tgtttcatca cagcaataat aaccagaact
     4801 aagacataga cttacccagg tttttggaaa aaaaattctg acaggagtga tatgacacat
     4861 tctcacttct aattggaaga tgtattccta acaatatatt tataatgggg taggaaacac
     4921 ttatacagaa agggcttagt cataccatgt tagtgccctg tcatggtaat aactggaagc
     4981 ccttgatatt ctttgaagga cataaaaaga attactagac tcctagtacc tgaaaagagg
     5041 acctacattg atttcgtctc agagaatttg taggtgtgtt tctcagaaca caatgtacta
     5101 aacatcataa tgcatgtttt tgttctcttc tttgttctct agccatggca ttaatgcttg
     5161 tgctgttctt cctggctgct gtactgcccc catcccttct tcaagataac tctcaggtat
     5221 gggtaaagtt tattattttc tcagaacaat ttatgctggt atgaaggcta ttcaagtaag
     5281 caactagtca actacatcac tcttcaattt tttttccttt tgattttttg agtcagggtt
     5341 tctctgtatg acccagaact cactctgtag accaggctgg ccttgaactc agaaatccac
     5401 ctgcccctgc ctcccatgtg ctgggattaa aggtttgtgc caaaactgct cagtcttcaa
     5461 aattttacaa gaccagagtg tgtagtaatc acaaagtact cattgatagt tcagaacagt
     5521 tgacattatt ttaaagttgt ggcaaaagaa cttgtcaccc taattacaag tgaatattaa
     5581 acatggttag ttaaattgat taaattaaaa ttgttatgtt tatgcaatat ctaaagaaac
     5641 aggcatgcat tcattacaca aagttattat caaagaaatg gctagagata aatatatcat
     5701 aataaatttt gtataaagaa gcattaagtt gtatttttgc aatatagttt taatataaat
     5761 attgatatta atttaaaata ccctttcctt tttaatgtgg tcaagagata aaacttacac
     5821 ataatatttg aactatattg ttattggatt tttcatgtct ctggatttat ttcctaatag
     5881 tttctctttt aagaattgtt attgatttat ttaatccatt tcagtgtggt cctcacagaa
     5941 acttcttatt acatttcaaa taacacaact tcaacatata tatatatata tatatatata
     6001 tatatatata tataaaatct cattttcata aatattgtgt gaaagattat ttgtctattt
     6061 tcaattttaa cagtttgcta gaatatagct agggatgata ctaaaaacac ttgcctaaaa
     6121 tgcacaaatc tctgggattg atcctcagca acacagacac acacacacac acacacacac
     6181 acacactcat tttcacacag agctgttaag tacatctaca atgcaaacaa aaaacatgag
     6241 ccaaatatta agtatttata atatgttcct tcatttagat attcccagtg aggattagcc
     6301 atttttttaa atgatggagt atgtgggact agcaaagata ttcccgatct tgttagaaag
     6361 ctactaaaag ctaaaagcct tgcacattgc tgtaaccctc gtactcttct ctggtctggg
     6421 cattggagac aactgaaact cttcaagaat agggagtgcc aattactgga caaacttgtt
     6481 ttggaatata agctttatgg tttagatttt ttcttaccat atccaaatta gaaatctaga
     6541 gactattttt gatctaggtg tttattgatg ttgttggtat tgctagtttc ttaaactgat
     6601 tctgttttat tgcacctttg aactatctat gctccctcca ttccttccta acacctcacc
     6661 tttttcttta gtctactgat atctcttcag agaatttcaa gatacaaata gcagagcaat
     6721 tcagagtcac ctttgatgaa tgaaggtttc tttgagagtt atcatgaaga actgatttct
     6781 ctgacattca tttccattcc tgtcttaaac ttgtgctgtt gtaattatta ggtcatatta
     6841 cctacccaat ataaaccttt taactagttt ttatccttac atttctgtgt taatagtatc
     6901 tctttaaaaa atattaccat tgtctgtcaa ggaaagagaa gcagacttat tgtctcttgg
     6961 agctttcagt aaccaaggaa ggtagaaaaa tgcagaaaat aataatggtt tcataaacta
     7021 tatattatgt ctttagaaag gtaacaacct taagtagggt tttcagttga aatggaatat
     7081 ctaaggagat attcctgagt ggataagcac acttactatt cttgcaaagt atattttggt
     7141 ttctattacc tatcgcaatt tgcctaaatt catctgtaac tccaactcca aaggatctac
     7201 tgtgctcctc tggcctctac atgcactttt ctcatctata caaacatata cacttagtta
     7261 aaaataaata cttttagaaa attatgcctt ttccttaata aataaatatt gaatgttatt
     7321 gttactttac tttataataa aatatgagga ttttccaaca atgacttaaa aacatacatt
     7381 ctttctgtct taaaaatcac tccttgttaa gaaaaaaaaa gaattcacta gcagtgagaa
     7441 agtccttggc tcaaggcatt cattgctgct ttcagaacat aaaacaacag ttagatgtgg
     7501 gcattttgca aatactggca atacagtatg tttagatcta tgtggcaaat gtctgcagaa
     7561 atttgaggga actagggact acatgaaaga gaaccaactt atccaagaag taagataaaa
     7621 tgtatgtgca ggtttgtcca ctcataatta agcaataaca tcctggtcta cactgtagaa
     7681 gaagagagga tttgaagtat acttccatca gcaaggggta ttaatatctc tgccaattct
     7741 tatatcactt aacctacctg tcccacacga acacaaaaat gtgtaactaa atatcagcat
     7801 ctgagttaac aacaggttta tttatgttca ttaaattgta aagccagtaa tactgaagaa
     7861 tttcaaaaac ccattgccta caaattgaca tagatattat attagacaat accctttgga
     7921 gtgatcacaa aatcgtatta catataaaat ttttcttact gtaatagata attgaatcca
     7981 cacatatagt gtgtctagac ttccatagtc tcacaagcct ccaaattctg tatcaaagtc
     8041 tgggtatctc tttcaaagat gtataataaa ttatgtgttt tattccttaa cacttatttg
     8101 ctatagccta attgtgtggg aatgacttaa ggttctctac catgcatgac tatcgttata
     8161 ttttcttaaa taaaatttga tatttgttgc ttagaaaagg tttttacttt ttttttttta
     8221 cttttacttt gacatgccat gaattcatgc tttattctca aaatttctgt ttaaaatgtt
     8281 tctgatgaaa agtaacagat gtttttaagt tttagtttca aggtcaatac ctaatattgt
     8341 ctgagcactt taataatgaa cttcattcat cttagccatc attttcacca ttttatgtaa
     8401 ccttatcata cactaatgtg actttcattt tatttccctt taggagaaca gtcttgagaa
     8461 actttcaacc agtaaaaaat cagtccaaga agagattgta agcaagcaca accaattgag
     8521 acgaaaggtt tctccatctg gcagtgactt actaaatatg gtgagggctc acagcagggt
     8581 agtgcatttg gagagctgtg tttggagcct gctgatagca tctgtgttat ctaagagtct
     8641 gccactattt tagcatctat tttttaaaaa tatgtattgt tttgttcatg tgcatgagta
     8701 tctatgtatg tgtttatata ttatgacgtg catgtaagat cagagctctt tctttccttt
     8761 gtcttcagcc atgatatgat tagagtagca tttactaaat tccctttata acagtggctc
     8821 actggcatta tggagctata tcaattacag ccacagaacc aaaaataaat ctttttactt
     8881 ttctctttag tgataaggca ctgtattatt tcctctccct ctcttaatct agggtcagaa
     8941 cataaccttt catctaaaaa gtcgtctgac ttggatatag atctaaagcc tatatttctt
     9001 ttaaattcat tcaacgtgta aaaagagaaa ctagtattta tgaggtaact ttttacaggt
     9061 gtgaaaataa tcttagtgtg tcccccatag ttagtgctcc ttgtagtaca gcaacaaggc
     9121 gcataacgat accacgatat caacaagttt gtacaaaaaa gcaggctggc gccggaaccg
     9181 aagttcctat tccgaagttc ctattctcta gaaagtatag gaacttcgaa ccctttccca
     9241 caccaccctc cacacttgcc ccaaacactg ccaactatgt aggaggaagg ggttgggact
     9301 aacagaagaa cccgttgtgg ggaagctgtt gggagggtca ctttatgttc ttgcccaagg
     9361 tcagttgggt ggcctgcttc tgatgaggtg gtcccaaggt ctggggtaga aggtgagagg
     9421 gacaggccac caaggtcagc cccccccccc tatcccatag gagccaggtc cctctcctgg
     9481 acaggaagac tgaaggggag atgccagaga ctcagtgaag cctggggtac cctattggag
     9541 tccttcaagg aaacaaactt ggcctcacca ggcctcagcc ttggctcctc ctgggaactc
     9601 tactgccctt gggatcccct tgtagttgtg ggttacatag gaagggggac gggattcccc
     9661 ttgactggct agcctactct tttcttcagt cttctccatc tcctctcacc tgtctctcga
     9721 ccctttccct aggatagact tggaaaaaga taaggggaga aaacaaatgc aaacgaggcc
     9781 agaaagattt tggctgggca ttccttccgc tagcttttat tgggatcccc tagtttgtga
     9841 taggcctttt agctacatct gccaatccat ctcattttca cacacacaca caccactttc
     9901 cttctggtca gtgggcacat gtccagcctc aagtttatat caccaccccc aatgcccaac
     9961 acttgtatgg ccttgggcgg gtcatccccc cccccacccc cagtatctgc aacctcaagc
    10021 tagcttgggt gcgttggttg tggataagta gctagactcc agcaaccagt aacctctgcc
    10081 ctttctcctc catgacaacc aggctcccag gtcccgaaaa ccaaagaaga agaacgcaga
    10141 tctctagagt caccgagttg ctttaccgga tgaagagggc cgaaacatac tgtccaaggc
    10201 ccttgctggc aatccaccca actgaagcca gacacaaaca gaaaattgtg gcaccggtga
    10261 aacagacttt gaattttgac cttctcaagt tggcgggaga cgtcgagtcc aaccccggac
    10321 cggtgcatgt ggaccccaag aaaaagcgga aggtggaccc taaaaagaaa cgcaaagtgg
    10381 atcctaagaa aaaaagaaag gtgcacgtgt ccatggtgtc caagggcgag gaactgttca
    10441 ccggcgtggt gcccatcctg gtggaactgg atggcgacgt gaacggccac aagttcagcg
    10501 tgtccggcga gggcgaaggc gacgccacat acggaaagct gaccctgaag ttcatctgca
    10561 ccaccggcaa gctgcccgtg ccttggccta ccctcgtgac cacactgacc tacggcgtgc
    10621 agtgcttcag cagatacccc gaccatatga agcagcacga cttcttcaag agcgccatgc
    10681 ccgagggcta cgtgcaggaa agaaccatct tctttaagga cgacggcaac tacaagacca
    10741 gggccgaagt gaagttcgag ggcgacaccc tcgtgaacag aatcgagctg aagggcatcg
    10801 acttcaaaga ggacggcaac atcctgggcc acaagctgga gtacaactac aacagccaca
    10861 acgtgtacat catggccgac aagcagaaaa acggcatcaa agtgaacttc aagatccggc
    10921 acaacatcga ggacggctcc gtgcagctgg ccgaccacta ccagcagaac acccctatcg
    10981 gcgacggccc tgtgctgctg cctgacaacc actacctgag cacccagagc gccctgagca
    11041 aggaccccaa cgagaagagg gaccacatgg tgctgctgga attcgtgacc gccgctggca
    11101 tcaccctggg catggacgag ctgtacaagc ctgcaggcga gggcagagga agtcttctaa
    11161 catgcggtga cgtggaggag aatcccgggc cgtccaattt actgaccgta caccaaaatt
    11221 tgcctgcatt accggtcgat gcaacgagtg atgaggttcg caagaacctg atggacatgt
    11281 tcagggatcg ccaggcgttt tctgagcata cctggaaaat gcttctgtcc gtttgccggt
    11341 cgtgggcggc atggtgcaag ttgaataacc ggaaatggtt tcccgcagaa cctgaagatg
    11401 ttcgcgatta tcttctatat cttcaggcgc gcggtctggc agtaaaaact atccagcaac
    11461 atttgggcca gctaaacatg cttcatcgtc ggtctgggct gccacgacca agtgacagca
    11521 atgctgtttc actggttatg cggcggatcc gaaaagaaaa cgttgatgcc ggtgaacgtg
    11581 caaaacaggc tctagcgttc gaacgcactg atttcgacca ggttcgttca ctcatggaaa
    11641 atagcgaccg ctgccaggat ataaggaatc tggcatttct ggggattgct tataacaccc
    11701 tgttacgtat agccgaaatt gccaggatca gggttaaaga tatctcacgt actgacggtg
    11761 ggagaatgtt aatccatatt ggcagaacga aaacgctggt tagcaccgca ggtgtagaga
    11821 aggcacttag cctgggggta actaaactgg tcgagcgatg gatttccgtc tctggtgtag
    11881 ctgatgatcc gaataactac ctgttttgcc gggtcagaaa aaatggtgtt gccgcgccat
    11941 ctgccaccag ccagctatca actcgcgccc tggaagggat ttttgaagca actcatcgat
    12001 tgatttacgg cgctaaggat gactctggtc agagatacct ggcctggtct ggacacagtg
    12061 cccgtgtcgg agccgcgcga gatatggccc gcgctggagt ttcaataccg gagatcatgc
    12121 aagctggtgg ctggaccaat gtaaatattg tcatgaacta tatccgtaac ctggatagtg
    12181 aaacaggggc aatggtgcgc ctgctggaag atggcgatct cgagccatct gctggagaca
    12241 tgagagctgc caacctttgg ccaagcccgc tcatgatcaa acgctctaag aagaacagcc
    12301 tggccttgtc cctgacggcc gaccagatgg tcagtgcctt gttggatgct gagcccccca
    12361 tactctattc cgagtatgat cctaccagac ccttcagtga agcttcgatg atgggcttac
    12421 tgaccaacct ggcagacagg gagctggttc acatgatcaa ctgggcgaag agggtgccag
    12481 gctttgtgga tttgaccctc catgatcagg tccaccttct agaatgtgcc tggctagaga
    12541 tcctgatgat tggtctcgtc tggcgctcca tggagcaccc agtgaagcta ctgtttgctc
    12601 ctaacttgct cttggacagg aaccagggaa aatgtgtaga gggcatggtg gagatcttcg
    12661 acatgctgct ggctacatca tctcggttcc gcatgatgaa tctgcaggga gaggagtttg
    12721 tgtgcctcaa atctattatt ttgcttaatt ctggagtgta cacatttctg tccagcaccc
    12781 tgaagtctct ggaagagaag gaccatatcc accgagtcct ggacaagatc acagacactt
    12841 tgatccacct gatggccaag gcaggcctga ccctgcagca gcagcaccag cggctggccc
    12901 agctcctcct catcctctcc cacatcaggc acatgagtaa caaaggcatg gagcatctgt
    12961 acagcatgaa gtgcaagaac gtggtgcccc tctatgacct gctgctggag gcggcggacg
    13021 cccaccgcct acatgcgccc actagccgtg gaggggcatc cgtggaggag acggaccaaa
    13081 gccacttggc cactgcgggc tctacttcat cgcattcctt gcaaaagtat tacatcacgg
    13141 gggaggcaga gggtttccct gccacagctt gatgaagatc tgagctctta attaatgatc
    13201 ataatcagcc atatcacatc tgtagaggtt ttacttgctt taaaaaacct cccacacctc
    13261 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
    13321 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
    13381 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatccgt
    13441 ttaaactaac tttaaataat tggcattatt taaagttaca tatggcggcc aattcgcctt
    13501 ctgcaggagc gtacagaacc cagggccctg gcacccgtgc agaccctggc ccaccccacc
    13561 tgggcgctca gtgcccaaga gatgtccaca cctaggatgt cccgcggtgg gtggggggcc
    13621 cgagagacgg gcaggccggg ggcaggcctg gccatgcggg gccgaaccgg gcactgccca
    13681 gcgtgggcgc gggggccacg gcgcgcgccc ccagcccccg ggcccagcac cccaaggcgg
    13741 ccaacgccaa aactctccct cctcctcttc ctcaatctcg ctctcgctct tttttttttt
    13801 cgcaaaagga ggggagaggg ggtaaaaaaa tgctgcactg tgcggcgaag ccggtgagtg
    13861 agcggcgcgg ggccaatcag cgtgcgccgt tccgaaagtt gccttttatg gctcgagcgg
    13921 ccgcggcggc gccctataaa acccagcggc gcgacgcgcc accaccgccg agaccgcgtc
    13981 cgccccgcga gcacagagcc tcgcctttgc cgatcctcta gagtcgagat ccgccgccac
    14041 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
    14101 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
    14161 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
    14221 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
    14281 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
    14341 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
    14401 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
    14461 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
    14521 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
    14581 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
    14641 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggccgacc gctatcagga
    14701 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
    14761 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
    14821 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
    14881 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
    14941 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
    15001 gcccaccccc cggatctaag ctctagataa gtaatgatca taatcagcca tatcacatct
    15061 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
    15121 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
    15181 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
    15241 tccaaactca tcaatgtatc ttatcatgtc tggatcccta actttaaata atgccaatta
    15301 tttaaagtta ggaattaatt cacccagctt tcttgtacaa agtggttgat atctctatag
    15361 tcgcagtagg cggaatatgg catacataag tgggaggaac tttggatata ggtcatgaaa
    15421 ctaccaagca gacaatgaca caggaaaaag gtactgtgga aggtatgtga gagagggcat
    15481 gggaggttga gcaggcgctg gaagaaggga gagaaaagca tttaaaaata aacttattat
    15541 aaatgtcaca atgaaagttg gtagtatata cactaacata aaattttaga tatatactat
    15601 tatgtttatt cattatgatg aaggtcatat ggtacctaca gaaatcttgc agtgatataa
    15661 gatagttcta ccagcctgcc tgcaaagata atgtgccttt gaagatgtat tattatttat
    15721 agctatctcc ttttttttct ctctctagga atggaactat gatgctcaag tgaatgctca
    15781 gcaacgggca gacaagtgta cattcagtca cagtcctata gaactcagga caactagtat
    15841 gtgaactcaa aatcacttat gtatgaatgg agaataacac aggcgggccc agtttcttgt
    15901 attgtattaa caaaactttt caaagtaaac acctattttg atactgtctt ttaaaagtag
    15961 ttaaacatct ctggaccaga atgtctcaga gaggttccat attagagatg gcgcaacgca
    16021 attaatgata acttcgtata gcatacatta tacgaagtta tggtctgagc tcgccatcag
    16081 ttcatgaagg atggaactac taattaaaat aacatgatgg ggcttgaaat tagaatctct
    16141 ttttaatttt atcctattag tttttattta tgggtatgtg tgtcttgggt atatcaatga
    16201 acctgtgaat gtatgtgctt aatcagaaga gtctctaaag atcaaaaggc attgtatttt
    16261 tgggaactga agttacagtg gttttaaccc atcccttatg gatgctacaa gttgaattca
    16321 attgtgctaa aggaacagga tatgctctta tctgttcagg ttcttttgca ggcttctgta
    16381 attagaattt ctaaattttg atcatttaag ccagtatctt atctaccttg ttttgttgtt
    16441 ttctttttct ttttatattg tttatttgtt tgtttgtttg tttttagtcg gggtttctct
    16501 ctgtattcca ggttgttctg gaacagagtc tgtagaccag gctagccttg aactcatgga
    16561 gatctgccta cctttgcttc tagagtgctg agattaaaga catgtacata tgtggtagct
    16621 tatctgatga tgtggcttgt tcatgtttat aagatatatt tttaattatc aaaaagtatt
    16681 ttatctgagc aactcatttt catttttttc tcagaaaata ccaaatattt tcattatctt
    16741 tcattacttc ttttattgtt tttgatttgg tggaatacag atctcactgt atagtacaag
    16801 atgtcttcaa atacataatc ttgtgtaaga cagcaatgtg tatgttccaa actttgtctt
    16861 catttttggt gacagctact tatgtacatt ctgggtagct gataagtata ttaagttaaa
    16921 aataaattat tagacatact ctttacatga ggaagtaatg aaagtatgag aacataaata
    16981 aatggacttt gagatatagt acttcttgaa acattaggaa tggtaatctc tggatgaatt
    17041 tgaaaatcat ttaattatcc attattaagg ctgtacttct tagctatcat tttaatcacg
    17101 tagtgtttaa aaagttttat tttaagtgct ttaacaatca gttaaagtat aaagtaattt
    17161 agaatttgca gagattgaga aaatactaat ctatttattg ggtgttagaa gttgtttttt
    17221 tctccattct tggaagaata tttagctaat gaaattttaa atttataact taaggcatga
    17281 cctataaatg ttaaagtatt gttccttcta tttttgattg ttttgtactt gtaagtaatg
    17341 actataaaga aggcttacac aaaacaatcg tgctaaatat gtaacaattc acataaaaat
    17401 ctctagttag aacagaagag aaattaaata agttagtttt ttgtcttttt gaagactcca
    17461 catcaacatt tcttgcatat gagacaaaca tacaatatta cacactccct cacacgtttt
    17521 cacacaggta tggagagttc aatgtgtcaa tgaatcaact taaggagaga agctatttag
    17581 aaataagact gctgtatcta tgtagttcat atgctgttca gcccacaaat tgcatgcctg
    17641 tggctactgt ccagacgaat gatcaagtct gaagcatgcc tctaatgaaa taatcaaaag
    17701 tcattgtgag caatgtcttt gaagagcagg aattctgctt tgtatagccc ctcccccctc
    17761 tctctcttct tttttctttc ctgctacctt aatgattggt ctatttcttt cccatagatt
    17821 taaaatgtgg tgagaatttg ttcatgtcat cttaccttgt accatggtct tctgtaatcc
    17881 aaggatggta taatgaatcc aaaggtctta tatttggtgt gggcccaaag caaaatgtta
    17941 gtgtggttgg acatcatact caggtaagaa acgatatttt ctctataaat gagaggcaaa
    18001 aaaattgttt gaattacttg atcagggctg cacatcagct ttgtttggct cctgaaggag
    18061 aatgagaaca atttcccaaa gttttctttc cagaacccct gacagctgat tgccagacaa
    18121 gtgaggtgac tgcattagca agtattatac aattgtgcct ccattcaggc cactgtcaaa
    18181 tagagcctac caaagctaag agagaaagta aagtgctaag gggattctga atggctgatt
    18241 ggaaagaaga tccagatatg gtataggcag atgtctaagt caagggttgg tttgtttgtt
    18301 tgtccaataa aacatcatga gaaaaagcag agtggggagg aaagggttaa tttggtttat
    18361 acttctaggt tgctgttcat caccaaagga agtgaggaca ggaacttaga cactgcagga
    18421 acctggaagc aggagctgat acagatgcta tggagggtgt tacttgtatg cttcctgtgc
    18481 cttgctcagc cctcttactt atagaatcta ggaccaccaa cccagggatg gcccaacaac
    18541 catgagctgg gaagtccttc attgatcagt aactatgact tacatctata tcttagggag
    18601 gcattttctc acctgaggct tcttcttctc tgatgactcc aactttgtca agttgctaca
    18661 caaaacaacc cgtacaactg tccccttgtc aagttgacac acaaccatat gcattacttt
    18721 taagcctcaa actttttttg ttatttatcc ccaagatctc acatttaaat ataaataact
    18781 ataaaaagtc ccacagtatc tacaaattca aaagcattaa aatttcaatc tccttaaaaa
    18841 ttcaaaatct cttctaaaag ttcaaagttt ttcagtagtc cattctggta aaaaactaag
    18901 ttaaatgtaa tgtatttttc aagagggaag aagcacagca tgatcacaat ctgaaacaag
    18961 caactgctaa acaccaacag tttttaaaac tcaatgccca gtgtctggga tccacacatg
    19021 accttctggg cacttccaaa tggcttggat cacctctcca tctctgtcca ctgtgactca
    19081 ggcagcttgt cttctatgct aatgctggct ccatttcact gatgttgctg ttattggtgg
    19141 tggtggaatg gtattttaat ttccaaaatg ctgaggtctg ctgctgcaac tgggctgtaa
    19201 tttcaggaat agcttttcat aggctctctt tctggtacca agcctcaaat tcattgcatg
    19261 accccttcag tcctgggcct tcaacttcca ctgaggtttc actttcatca atggcctctc
    19321 ctggcctcta agaatgccaa actttagata ttttccatga atcttccata ccttcaaaat
    19381 tagtaacatt gccaagtcta gtaggcagcc aaaggtacaa ctttggccac ataatctgga
    19441 atgcagcttc tctgtgctct cagggaaaca cttcccagca gatttcacct tagtaatgat
    19501 agtcacttct taatcatcac taatcttatc tccagctgac caacatcaac tgtccccaaa
    19561 gcgtaaaggt ttacttcagt agttctggtg tctaattagt aacagctgat tttttttttt
    19621 ttttgctcta gctaagaaga actgcagaat cttaaagcaa aatagaaaat gaccctaata
    19681 cagtctttaa tcttcccttt gaaatttcac atgccaggcc tccctcttgg gtactgttca
    19741 acattcttat cttccaagct cctaaatctt ccctcagatc ttttaatata caatggcttt
    19801 tctagctcaa agttccaaag tcctttcaaa atcctcccta aaatatggtc agggatgtca
    19861 cagcaatact gcactatggt ttttaccaaa atctgcaata tattcagggt tctctagagt
    19921 cacataactt atggatagaa tcaatctctc tctctctctc tctctctctc tctctctctc
    19981 tctctctctc tgtctctttc acatttatat ttgagatcct aaaaggatgt acctcaaggg
    20041 atactgctac ctattttaaa tttataatgc atttagggat gtattagggg tagctatatc
    20101 atattacaca ttattgtgac ctaatttttg tttatacatt tgtgattcta caaaggatag
    20161 tcatatcttt ttaggcatta ttatcaccta gctattgttt gcatttggac ctgaaatatg
    20221 atttgtgtca aaatgacaag ggatagatta tgattggttg ccaacatgat catatctgga
    20281 atgaactata accaaaaaag aacacatacc tgttttctgg atctgaaggc taaaagacaa
    20341 tatgtctttt ttcatgattt tgagtttgga agacacaagc ttttgatgaa gatactgagg
    20401 tgggatcata cacacacaca cacagacaca cacacacaca cacacacaca cacacacaca
    20461 cacacacaca cacacttttt gatctgtatc aacatatgtc tttaatccag atcttgaggc
    20521 ataactttaa tctgggccat atcttctgct agaggcctat ataaggacaa tgaaaaaagg
    20581 aaagtttctt tcactcttta cctgcttata ttttcttgtc cagcatattc actggagtct
    20641 acttctttag gattctgggt tatacacaag accagttgaa atatgtatca atgtggtatt
    20701 gaacaactac tagatttttg gacttcccat tcacagctag tcattgcttg gttagttgga
    20761 ttgcagtctg taagtcattt caataaacat acacattcat ttcataagtt ctctgactct
    20821 agagatcctt gactaatgta gcagcaagca actccaattc cttgatctga tcagagatac
    20881 tggaactgaa acctggtttc aggaaggaac cagaaaattc aagatcagat ctctacacgg
    20941 aagcactcac tgcacacaac tatcaggaaa tataaatgat tactgagtgg tagcctttgt
    21001 ccagtcttga ccagaaaggt ttgcatgtca gtcatgaaat ctgtccaatt gggaaggcag
    21061 atactcctgg ccatgtgtta gcattcaact gaacacctct tctgtgattc tgggaggaat
    21121 tcatggaccc tttgatggct gacgaggagt agacataata tccaggttac tctcttgtaa
    21181 tttattgtca tgttc
//