LOCUS       239511#pL1L2GT0_LF2A_nEGFPOT2A_CreERT2_pAbActNeopA#L3L4_pD223_DTA_T_spec        22774 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2GT0_LF2A_nEGFPOT2A_CreERT2_pAbActNeopA
COMMENT     design_id: 239511
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000007449
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            5074..5215
                     /db_xref="ENSEMBL:ENSMUSE00000192313"
                     /rank="1"
                     /note="ENSMUSE00000192313"
     exon            8060..8227
                     /db_xref="ENSEMBL:ENSMUSE00001023904"
                     /rank="2"
                     /note="ENSMUSE00001023904"
     exon            8858..8974
                     /db_xref="ENSEMBL:ENSMUSE00001061621"
                     /rank="3"
                     /note="ENSMUSE00001061621"
     misc_feature    4275..9532
                     /note="5 arm"
     misc_feature    9560..9584
                     /note="B1 Gateway"
     misc_feature    10551..10554
                     /note="FCHK QC critical region"
     misc_feature    10535..10550
                     /note="FCHK QC prefix region"
     misc_feature    10555..10560
                     /note="FCHK QC suffix region"
     misc_feature    10683..10734
                     /note="F2A"
     misc_feature    10560..10682
                     /note="1Dleader"
     primer          10420..10441
                     /vntifkey="27"
                     /dnas_title="seq primer FCHK2"
                     /label=seq primer FCHK2
                     /note="seq primer FCHK2"
     exon            10520..10559
                     /vntifkey="61"
                     /dnas_title="mouse En2 exon"
                     /label=mouse En2 exon
                     /note="mouse En2 exon"
     misc_feature    11606..11606
                     /note=" A to G to kill RsRII site"
     misc_feature    11556..11609
                     /note="self-cleaving' 2A peptide"
     misc_feature    11610..12635
                     /note="Cre"
     misc_feature    10740..10829
                     /note="3X NLS"
     misc_feature    10830..11546
                     /note="EGFP"
     primer          complement(9713..9734)
                     /vntifkey="27"
                     /dnas_title="LRPCR LAR3_EN2"
                     /label=LRPCR LAR3_EN2
                     /note="LRPCR LAR3_EN2"
     intron          9645..10519
                     /vntifkey="21"
                     /dnas_title="mouse En2 intron"
                     /label=mouse En2 intron
                     /note="mouse En2 intron"
     primer          complement(9609..9632)
                     /vntifkey="27"
                     /dnas_title="FRT3_oligo"
                     /label=FRT3_oligo
                     /note="FRT3"
     misc_feature    9597..9644
                     /vntifkey="21"
                     /dnas_title="Frt"
                     /label=Frt
                     /note="Frt"
     misc_feature    13896..13903
                     /vntifkey="21"
                     /dnas_title="polylinker"
                     /label=polylinker
     misc_recomb     13864..13895
                     /note="Rox(inv)"
     misc_feature    13856..13863
                     /vntifkey="21"
                     /dnas_title="RE sites"
                     /label=RE sites
     primer          13796..13814
                     /vntifkey="27"
                     /dnas_title="seq primer NF"
                     /label=seq primer NF
     primer          13670..13693
                     /vntifkey="27"
                     /dnas_title="LRPCR RAF5"
                     /label=LRPCR RAF5
     misc_feature    13613..13855
                     /vntifkey="21"
                     /dnas_title="SV40 pA"
                     /label=SV40 pA
     misc_feature    12645..13589
                     /note="ERT2"
     misc_feature    14691..15273
                     /note="synthetic fragment mutates RsRII"
     misc_feature    15103..15103
                     /note="G to C conservative mutation to kill RsrII site"
     misc_feature    15451..15694
                     /note="SV40pA"
     misc_feature    15509..15532
                     /note="RAF5"
     misc_feature    15258..15277
                     /note="neoBstB1_5"
     misc_feature    14965..14983
                     /note="neoSphI_5"
     misc_feature    14628..14647
                     /note="neoPstI_5"
     misc_feature    14459..15444
                     /note="neomycin resistance "
     misc_feature    14309..14328
                     /note="betact5"
     promoter        13913..14433
                     /note="human beta actin promoter"
     insert          13610..15689
                     /source="pL1L2_GTIRES_BetactP FLFL MapVerified"
     misc_feature    15690..15694
                     /note="for bActNeo"
     misc_recomb     15696..15727
                     /note="Rox"
     misc_feature    15740..15760
                     /note="B2 Gateway"
     misc_feature    9533..15790
                     /note="Synthetic Cassette"
     exon            16209..16286
                     /db_xref="ENSEMBL:ENSMUSE00000293604"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 1 ENSMUSE00000293604"
     exon            17920..18042
                     /db_xref="ENSEMBL:ENSMUSE00001061087"
                     /type="targeted"
                     /rank="5"
                     /note="target exon 2 ENSMUSE00001061087"
     exon            18334..18440
                     /db_xref="ENSEMBL:ENSMUSE00000293591"
                     /type="targeted"
                     /rank="6"
                     /note="target exon 3 ENSMUSE00000293591"
     misc_feature    15791..18555
                     /note="Critical Region"
     misc_feature    18579..18612
                     /note="Downstream LoxP"
     misc_feature    18556..18635
                     /note="synthetic loxP region"
     exon            18910..18991
                     /db_xref="ENSEMBL:ENSMUSE00001036753"
                     /rank="7"
                     /note="ENSMUSE00001036753"
     exon            19437..19520
                     /db_xref="ENSEMBL:ENSMUSE00001031392"
                     /rank="8"
                     /note="ENSMUSE00001031392"
     exon            20054..20136
                     /db_xref="ENSEMBL:ENSMUSE00000192320"
                     /rank="9"
                     /note="ENSMUSE00000192320"
     exon            21837..22133
                     /db_xref="ENSEMBL:ENSMUSE00000366485"
                     /rank="10"
                     /note="ENSMUSE00000366485"
     misc_feature    18636..22774
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggaccttat aaaccatcct tgcgtttcac agttgagtac atgacatttc
     4321 atagtcacaa aagattgttt tcaaatcagg cacagttttt tttttttttt aatgtcgtta
     4381 taaaacacaa ttggtcaaat ccctaaatct gaaagaaaag aaaaggaaga agaagaagaa
     4441 aagtaggcaa cgaggaagaa aggaaaaaca aaggaaggag aaagagaatt tcattaggaa
     4501 agaacctgtc ccagtggcta gcaggtcatt ttcatcctca gggaatcttc ttgccttgca
     4561 atctgacaga aaacgcacag atacaggcat ttctagaaag gtgtccatga cctacattta
     4621 aaaaaaaaaa aaatctttcc acaaggtcac ctgccagttt cctctgtaac agacatccct
     4681 tagttttgtg gccaaggaca gttatttcaa agtagttact gtaaaagatg tctcattttt
     4741 cctcgacatt tttatttgcc tcaaactgca tgagcaccca cgcagttcac tagcgttata
     4801 tccactccca gggcaatgac ccttcccaaa taaacttacc agttcatctt ggatgacctt
     4861 tcaaccatgt cacttaggtt cacaaacctc tgatccgcat acccaataaa gcatgcattc
     4921 tgcccagata gcctttgcac tgtgctgact ttaatctttg tttacgatga tcagtgtggc
     4981 acaagattga catgaagttc ctgttctcat gccccaaccc ttggctgtgg ctgcttgcca
     5041 atgtggtaag agcccgcctc ctttatccag gacataaaaa tgtcaccagc ctccttgtag
     5101 agaaagcagg catttcatct gctcactcag agaactctct gcagcaacca tggccacctt
     5161 cgtggaactc agtaccaaag ccaagatgcc ccttgtgggc ctgggcacct ggaaggtgag
     5221 tgtgcagtct tgggacacct gctgcctttg aggggaggtc tggacatttt cttttctgtg
     5281 cagagcatct atctgccttg ggtttgataa ggccagcatt atcacctgtt gtcagctgct
     5341 ttgggttgca gatgagcctt tcccagaaat gacaatagct tccttggcga atatcaggtt
     5401 taaatttgta caatttttca gggagaaaac tgttttcagg gaagttttct cttagaaagc
     5461 ctttccttgg gactctgagg gtctctgttg gtttgttact ggctgagtga ctgagagaga
     5521 gtatagagtt cttaatgacc ccactcttat gatgcaagac tatgccaaca tctgagtggc
     5581 ctgggagctt gtgggaaagg cagaggacaa ctggactggc ccaggcagtt tacaaaatct
     5641 ccatgtgatt cccccacacc ttaagaccca tgcagcatga gagccacaga attggagtca
     5701 gctcaaagtc tgccctggct gtgttaatct ctactggcaa gttgctctgt ggcccatccc
     5761 agtacccagt gaaacagatt tctcacgaaa tcttgggaca agtctgacat tggtccatct
     5821 tcttctaact ctgccttctt tgctgtttct attactgaca acatgagagt agccagcttg
     5881 ttaaccagga gctgttttta gttttgattt tgatttcttg agacagggtt tctctctctc
     5941 taacctaaaa tgaccttgat caaatttgga gtaatcctcc tgcctcagct tcttgaatac
     6001 tgggattata ggtgtgagcc ttcatccttg gctcaggagt agtttgggaa cccatctgtc
     6061 ttagttaggg ttttactgct gtgaatagac accacgacca aggcaagtct tataaaaaac
     6121 aacatttaat tggggctggc ttacaggttc agaggtttag tccattatca tcaaggtggg
     6181 agcatggcag tatccaggca ggcatggcac aggcagagct gagagttcta tgttttcatc
     6241 caaaggctgc tagtggaaga ctgacttcct ggcaactggg gtgagagtct taagtccaca
     6301 cccacagtga cacacctact ccaaccaggc cacacctcca gacggtgcca ctccctgctc
     6361 caagaatata caaaccatca caccatctgt cctgtgtcca agactcattt ccagtcttcc
     6421 tctgtttctc ttttctttac acagagggta ggttctctct ttgtttctac attagattgg
     6481 ggtcttacta gaatggtgag acttgcaacc agagtagaaa tttcacattc cctcaaggtg
     6541 acaaataatt acaaatctcc aggatactca ccctggacaa atttagaaga aatgcacttt
     6601 cagagttcca aaagcatttg taaatatttg taagaagttg ttttcgaggt aaggactctg
     6661 tgctcaccag tggacaagca ggagggtgac actttggccc tgggccccag tgcttttgtg
     6721 tttctggaaa ttccttctcc tcatgatggc agagctgttg atctctgctc tctgtgcttc
     6781 tcaaggtttg atgcttaaaa acagaacctt tagatttagt aataggggaa gagttattta
     6841 tgggctgttg tttgagagtc ttccacagcc ctggttacct tataataatt cattatgtgg
     6901 ctgagactgg ccctgaactc ctggttttcc tactcacctc ccaaatgcta ggattaaaac
     6961 atgggccacc acatccagtg agatgttgtt ttgtattgac tgagataata ttgtgcccca
     7021 ggctgtcctc aaagccacag cctggccctc ctacctttgt gcctctcatg gtggtgttac
     7081 agccatgcag caccatgctc tgcagagagg tacgtgatga gtacttactc tcctaagcca
     7141 atacctggct tggttaggtc aggacagaag gtgacccact tcaaatgctc cttcccttgg
     7201 ctccttggct tgttttctct ttctttctct ctctttctct ctctctctct ctctgtctgt
     7261 ctctgtctct gtctctgtct ctgtctttgt ctctgtctct gtctctgtct ctctctctct
     7321 ctctctctgt agtccatatg gggaaaatcc atttttataa agtttctgtt aggtttaaaa
     7381 ctgaggacaa agaaatgaag tgcctattta acatcttcct gtttgctctt tttctgaaac
     7441 agtgattaca tattggattg caagtgccag gtgatgtgct gaaatcttca caatcttgtg
     7501 gcaagctggc aacacaaagg cctcatgggg cagggcccat aacttgctat gttttgctgt
     7561 caaggtttag ggaaattgag gaatctgctc agcctcatac tgcaccatag cagagttcaa
     7621 atcacattgc tataatatat tttttaaaga tccttctaac tgtctggagt ctgagaactc
     7681 ctcctcagaa tctagttttc aggctagcct tgcaagtttc aaggggagtc atttgtcact
     7741 cagaaactgg aggcacttca agatcaacct ggactggagg actagaatag gacacgagtc
     7801 agaatcatga caatgacatg tgtattaaga tgccataatg aaatccatca ctttgtatga
     7861 taacctaaaa atttagtgaa ataaaaaaag taattcactt tttcttttcc tgggagaaac
     7921 ccctcagtgt atgttttctt ctcccaagtc cacttgggaa caggactgca gcaaggctgt
     7981 ggttttcaga atctggtcta tgtggagccc ttaagaagag gccttggagc atctaatggt
     8041 cttttctatt gcctttcagt cttccccagg ccaagtcaag gaagccgtga aggcggccat
     8101 tgatgctggg tatcgccaca ttgactgtgc ctatgtgtat cacaatgaga atgaggtggg
     8161 agaagctatc caagagaaga tcaaagagaa tgctgtgaag cgggaggatc tcttcatcgt
     8221 cagcaaggta caatgatgca cttggtggac aagacttcaa ggcaggtgga agtatctgtg
     8281 taggggcagg tttgagagct tattcgcctt tctttgtggg cctgcatgtc tcctggccct
     8341 ttgttccctg ctttcattat gtctcttggt agaattccac acacaaaaac atccactgtt
     8401 ggagtggcca cacctatata tgtgtatata ggtatatgtg tatatgctca ttgccattca
     8461 tcacagggcc tgacaggcct ggggataagg gagtactaaa aagggccagc cctcctattg
     8521 catcgtaata cttgaacttg aagagccaca aacaactcct gccatctgag aaatgagagc
     8581 cacagaaaag catgtgacac aagaataatt ctagtcaacg gccgactaag ttaaatgact
     8641 aagaaacaaa cccaatatcg agagcatagg ggaacactta ggaagtgatg ttgacttgct
     8701 aagagctgat gataagaaat acgttgcatt tcttaacgta tttcttataa gtgaatagcc
     8761 ggtcctgggg tgtacaaatc tccaagcagg aggagtaact gtagtatgtg tggccttcct
     8821 ctccctagtt ctaatgctcc cctgttacac cctgcagctg tgggccacat tctttgagaa
     8881 aagcctggtg aagaaagcct tccagaacac cctctcggat ctgaagctgg actatctgga
     8941 cctgtatctg gtccactggc cacagggatt tcaggtttga gctgctcttt gccttctatt
     9001 tttggggaga tgctacaaag tggctatggt gccaagactg tgacactggg gagggatgaa
     9061 gcagggggtt gggtttgagt tttattctat tttttgtata tgagcgtttt gtttacatgt
     9121 atatgtgtgc accatgccta tgcttggtgc ctgtggagat cagaagagag catgggatcc
     9181 ctgggaattg gagttatgga tggttgtgaa acaccacctg ggtgctagga acccaacctg
     9241 ggtcctctgc aagagcagca agtgctctca tcttctgacc cacctctcta gtccccagaa
     9301 gactgtgcag cgcggaggct gaggcaacag gtggtacttg gtgagactca tgcttggttt
     9361 tctgcaattg tgtttgaaag aaacagtatt tcttctacca gctcagataa gctgcaactg
     9421 gccagccatc ttcctcttgg atgcatgccc atgagtgccc ttggtaggat gtcttggggc
     9481 actcttgcat aagttcctat taatcaaagt gtgcccagag agtgacctcc ataaggcgca
     9541 taacgatacc acgatatcaa caagtttgta caaaaaagca ggctggcgcc ggaaccgaag
     9601 ttcctattcc gaagttccta ttctctagaa agtataggaa cttcgaaccc tttcccacac
     9661 caccctccac acttgcccca aacactgcca actatgtagg aggaaggggt tgggactaac
     9721 agaagaaccc gttgtgggga agctgttggg agggtcactt tatgttcttg cccaaggtca
     9781 gttgggtggc ctgcttctga tgaggtggtc ccaaggtctg gggtagaagg tgagagggac
     9841 aggccaccaa ggtcagcccc ccccccctat cccataggag ccaggtccct ctcctggaca
     9901 ggaagactga aggggagatg ccagagactc agtgaagcct ggggtaccct attggagtcc
     9961 ttcaaggaaa caaacttggc ctcaccaggc ctcagccttg gctcctcctg ggaactctac
    10021 tgcccttggg atccccttgt agttgtgggt tacataggaa gggggacggg attccccttg
    10081 actggctagc ctactctttt cttcagtctt ctccatctcc tctcacctgt ctctcgaccc
    10141 tttccctagg atagacttgg aaaaagataa ggggagaaaa caaatgcaaa cgaggccaga
    10201 aagattttgg ctgggcattc cttccgctag cttttattgg gatcccctag tttgtgatag
    10261 gccttttagc tacatctgcc aatccatctc attttcacac acacacacac cactttcctt
    10321 ctggtcagtg ggcacatgtc cagcctcaag tttatatcac cacccccaat gcccaacact
    10381 tgtatggcct tgggcgggtc atcccccccc ccacccccag tatctgcaac ctcaagctag
    10441 cttgggtgcg ttggttgtgg ataagtagct agactccagc aaccagtaac ctctgccctt
    10501 tctcctccat gacaaccagg ctcccaggtc ccgaaaacca aagaagaaga acgcagatct
    10561 ctagagtcac cgagttgctt taccggatga agagggccga aacatactgt ccaaggccct
    10621 tgctggcaat ccacccaact gaagccagac acaaacagaa aattgtggca ccggtgaaac
    10681 agactttgaa ttttgacctt ctcaagttgg cgggagacgt cgagtccaac cccggaccgg
    10741 tgcatgtgga ccccaagaaa aagcggaagg tggaccctaa aaagaaacgc aaagtggatc
    10801 ctaagaaaaa aagaaaggtg cacgtgtcca tggtgtccaa gggcgaggaa ctgttcaccg
    10861 gcgtggtgcc catcctggtg gaactggatg gcgacgtgaa cggccacaag ttcagcgtgt
    10921 ccggcgaggg cgaaggcgac gccacatacg gaaagctgac cctgaagttc atctgcacca
    10981 ccggcaagct gcccgtgcct tggcctaccc tcgtgaccac actgacctac ggcgtgcagt
    11041 gcttcagcag ataccccgac catatgaagc agcacgactt cttcaagagc gccatgcccg
    11101 agggctacgt gcaggaaaga accatcttct ttaaggacga cggcaactac aagaccaggg
    11161 ccgaagtgaa gttcgagggc gacaccctcg tgaacagaat cgagctgaag ggcatcgact
    11221 tcaaagagga cggcaacatc ctgggccaca agctggagta caactacaac agccacaacg
    11281 tgtacatcat ggccgacaag cagaaaaacg gcatcaaagt gaacttcaag atccggcaca
    11341 acatcgagga cggctccgtg cagctggccg accactacca gcagaacacc cctatcggcg
    11401 acggccctgt gctgctgcct gacaaccact acctgagcac ccagagcgcc ctgagcaagg
    11461 accccaacga gaagagggac cacatggtgc tgctggaatt cgtgaccgcc gctggcatca
    11521 ccctgggcat ggacgagctg tacaagcctg caggcgaggg cagaggaagt cttctaacat
    11581 gcggtgacgt ggaggagaat cccgggccgt ccaatttact gaccgtacac caaaatttgc
    11641 ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg gacatgttca
    11701 gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt tgccggtcgt
    11761 gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct gaagatgttc
    11821 gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaactatc cagcaacatt
    11881 tgggccagct aaacatgctt catcgtcggt ctgggctgcc acgaccaagt gacagcaatg
    11941 ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt gaacgtgcaa
    12001 aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc atggaaaata
    12061 gcgaccgctg ccaggatata aggaatctgg catttctggg gattgcttat aacaccctgt
    12121 tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact gacggtggga
    12181 gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt gtagagaagg
    12241 cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct ggtgtagctg
    12301 atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc gcgccatctg
    12361 ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact catcgattga
    12421 tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga cacagtgccc
    12481 gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag atcatgcaag
    12541 ctggtggctg gaccaatgta aatattgtca tgaactatat ccgtaacctg gatagtgaaa
    12601 caggggcaat ggtgcgcctg ctggaagatg gcgatctcga gccatctgct ggagacatga
    12661 gagctgccaa cctttggcca agcccgctca tgatcaaacg ctctaagaag aacagcctgg
    12721 ccttgtccct gacggccgac cagatggtca gtgccttgtt ggatgctgag ccccccatac
    12781 tctattccga gtatgatcct accagaccct tcagtgaagc ttcgatgatg ggcttactga
    12841 ccaacctggc agacagggag ctggttcaca tgatcaactg ggcgaagagg gtgccaggct
    12901 ttgtggattt gaccctccat gatcaggtcc accttctaga atgtgcctgg ctagagatcc
    12961 tgatgattgg tctcgtctgg cgctccatgg agcacccagt gaagctactg tttgctccta
    13021 acttgctctt ggacaggaac cagggaaaat gtgtagaggg catggtggag atcttcgaca
    13081 tgctgctggc tacatcatct cggttccgca tgatgaatct gcagggagag gagtttgtgt
    13141 gcctcaaatc tattattttg cttaattctg gagtgtacac atttctgtcc agcaccctga
    13201 agtctctgga agagaaggac catatccacc gagtcctgga caagatcaca gacactttga
    13261 tccacctgat ggccaaggca ggcctgaccc tgcagcagca gcaccagcgg ctggcccagc
    13321 tcctcctcat cctctcccac atcaggcaca tgagtaacaa aggcatggag catctgtaca
    13381 gcatgaagtg caagaacgtg gtgcccctct atgacctgct gctggaggcg gcggacgccc
    13441 accgcctaca tgcgcccact agccgtggag gggcatccgt ggaggagacg gaccaaagcc
    13501 acttggccac tgcgggctct acttcatcgc attccttgca aaagtattac atcacggggg
    13561 aggcagaggg tttccctgcc acagcttgat gaagatctga gctcttaatt aatgatcata
    13621 atcagccata tcacatctgt agaggtttta cttgctttaa aaaacctccc acacctcccc
    13681 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
    13741 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
    13801 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccgttta
    13861 aactaacttt aaataattgg cattatttaa agttacatat ggcggccaat tcgccttctg
    13921 caggagcgta cagaacccag ggccctggca cccgtgcaga ccctggccca ccccacctgg
    13981 gcgctcagtg cccaagagat gtccacacct aggatgtccc gcggtgggtg gggggcccga
    14041 gagacgggca ggccgggggc aggcctggcc atgcggggcc gaaccgggca ctgcccagcg
    14101 tgggcgcggg ggccacggcg cgcgccccca gcccccgggc ccagcacccc aaggcggcca
    14161 acgccaaaac tctccctcct cctcttcctc aatctcgctc tcgctctttt tttttttcgc
    14221 aaaaggaggg gagagggggt aaaaaaatgc tgcactgtgc ggcgaagccg gtgagtgagc
    14281 ggcgcggggc caatcagcgt gcgccgttcc gaaagttgcc ttttatggct cgagcggccg
    14341 cggcggcgcc ctataaaacc cagcggcgcg acgcgccacc accgccgaga ccgcgtccgc
    14401 cccgcgagca cagagcctcg cctttgccga tcctctagag tcgagatccg ccgccaccat
    14461 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
    14521 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
    14581 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
    14641 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
    14701 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
    14761 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
    14821 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
    14881 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
    14941 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
    15001 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
    15061 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gccgaccgct atcaggacat
    15121 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
    15181 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
    15241 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg
    15301 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
    15361 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
    15421 caccccccgg atctaagctc tagataagta atgatcataa tcagccatat cacatctgta
    15481 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
    15541 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
    15601 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
    15661 aaactcatca atgtatctta tcatgtctgg atccctaact ttaaataatg ccaattattt
    15721 aaagttagga attaattcac ccagctttct tgtacaaagt ggttgatatc tctatagtcg
    15781 cagtaggcgg tttttgctgt aggcccttat gaacagcctt ggtgagagtg gcatggtctc
    15841 caaactcagg ccttctccag gttgcaaggt taggagaagc tggttactct cttcctcaga
    15901 taaccatact cttctgatat aaaacctctg cctagtgacc ttgaagttta cattggttaa
    15961 cttccacgat gatagtgatg gcttctataa tatactagag gacttggagc cagctgagta
    16021 ttgccagaaa tagaggactg acttcccatg gtagactgac acaggaattt tttttgaata
    16081 ttatattttt atgtgagttc tctggaattt gacatgggta cttcttattt cgccgtcctg
    16141 agacgtctct tcacaggacc aggtaactaa atgccgattc tggtgatatt tttgtttctc
    16201 atttacaggc tgggaatgcg ttattaccca aagacaataa aggcaaagtt ctcctgagta
    16261 aatccacatt cttggatgcc tgggaggtag gttccaactt ccttcaagtg tgctgctcaa
    16321 agttaaaata caaatcttct ctcagatgct gttgagactc caccctggta tggaaaggct
    16381 caagtgctct gtatataatt tcagaatgct tattctctag ttccccctct gtcctggaaa
    16441 gtggtgtctg tggagctact tgtgaaagtc agagggcagc tacccaaagt aggttctttc
    16501 cttctatcac ataggctcca ggaagaaact gtgaccatct agcttggcag caaggacctt
    16561 tacccactga aacatttccc tggccccatg aaacactttc aggaacaaca acaacaacaa
    16621 aaaaaccaac caaccaaaca aacaaaacct tactgcaagt cagaggctct gatagataca
    16681 agaaggatca agtatgtcag taaatgaaaa ataaaataaa aaggaaggac atgactgctc
    16741 ttaggtatgc tggaggagaa agtttactca gagtattcca agttgtagcc aaagatgaga
    16801 tttcacgcgt cagctaaacc ctccttgctc gtccctgcct ctaagccctt gctgtccctc
    16861 ccccatccgt gcatgatttt actacctttc ttcatgtcaa ttttttttca ggctcaattt
    16921 aaactctcct cccaatgact ctcctgggct aagatgattc cttcctctcc tccctcatcc
    16981 atgtactaat tgatgtcaaa taagctgagg cagcctagca agcacattgt atacatttac
    17041 acacacacac acacacacac acacacacac acgtatatgt atatatatat atgtatatat
    17101 atatatatac acatgcacaa tatgatgtat atgtagtata tgtatatgca tatgtataca
    17161 cacatataaa cagaaactta gaatggatga ataattataa attcacattc aatgtcacta
    17221 tcaggttgga taattatctt tgggaaagtt taaaacaagc tcaatgtatt taagtaataa
    17281 aaaaataaac tccagatcct tgggtggatg tgatatgggt tggctgggtg cccctgcctg
    17341 ggtccatctg tctgcaaggt gatccttact gtatgctttc aatcacggct atgtgagagg
    17401 ctgaggcaca aagatcacat ttcagatcct gcctggactt cagagtaact tcaaggccaa
    17461 cccttacaag atcctattca agattggaaa atgaaggggg ttaggaacta gagatacaga
    17521 ttcacggcag attgcctgtc taacacatag gagaccacag actcaaccct taataagcac
    17581 aaaataaaaa tcaaaaacca aagagagacc acagggacga ttcctttcct tacactgtgg
    17641 actgtttcct ggataaccta catcactcag tttgggtcac ttttgcctat gagcagacag
    17701 catataaaac cgttacttct ccttcctcat tacatctgag ctggggaaga gtaggatggc
    17761 tggaagcagc catgagggca ccacagtcta gactaaaatt ctcctttagt gcttcactta
    17821 atttgccacg agggagccat tcctcctacc aaggactaag cacgccagca tccctgtatc
    17881 ctcttagagc catctaaatg atattcttca ctgtggcagg ccatggagga actggtggac
    17941 caggggctgg tgaaagctct gggcatctcc aacttcaacc acttccagat tgaaaggctc
    18001 ctgaacaagc ctggactaaa acataagcca gtgaccaacc aggtaagttc tgtttggggt
    18061 gggtcctgct ctgtgcctgc ttcagcactg ggaggcagag tctcctctca gaggtctatg
    18121 ttcaggatgc ttagggaggt gggaggcagg tctctgggtc tggtttgtag catacatacc
    18181 tgtccatggg ttgtgtttgg cctttgtgtt ggtcctttca tgtgtatgga gttcctgctg
    18241 agccattcag gaagacctcc ttccctagag atggtgatgt ttgttttttt tttttttttt
    18301 tttgtttttt tttttttttg ttttctcttg cagattgaga gccaccctta tctcacccag
    18361 gaaaaactga tccaatactg tcaatccaag ggcatcgctg ttacagccta cagtcccctg
    18421 ggctccccag acaggcctta gtaaggcttc agcatactgg ttctttcttt tttttttttt
    18481 atccttctta aaattagcat tttgtaattt gttctattag tatgtaagtt ccaaggcaca
    18541 agataaaata taccagagat ggcgcaacgc aattaatgat aacttcgtat agcatacatt
    18601 atacgaagtt atggtctgag ctcgccatca gttcataacc caagtgggat tgtcagggta
    18661 aagtgttttt aaaatgatag catgtctgat cccctggaat attccatttc tgatgtcctg
    18721 tgctggggca gtattttcaa attgccaaag tactagatcc tatgcacgaa tccaggggga
    18781 aagatcttcc tgcctgcctt gcagaggaaa aatgctgcag tgctcagcct tgatgctgtc
    18841 ctggcaggga tgttcacggg cacacagtag ccatggtgat ttttactcca gctctctttc
    18901 ttcgtctagt gccaagccag aagaccccgt agtaatggag attcccaaga tcaaagagat
    18961 tgctgcaaaa cacaagaaaa cagtagctca ggtaaaataa agtatttccc ccttttattt
    19021 agcaatccag tcttgtagtc atgtgtacaa ctgtgttgct tgcttttaat tccctggaga
    19081 ggaagaaatg ggaagtgtaa gcctgttggg agcttcagaa aataccgcta gcattagtga
    19141 agcaaggatc tgcctctgtc tttcagattc ctgccacacc ctcaggcgtt gggttcaagc
    19201 gccaactata tagggtctaa gcctatgact acaggctact ctcctcactc ctcgttagga
    19261 attcctagtt gtttcttgga tcaaggggca taagaaggga actggaaaag tatccctaat
    19321 ggtgaagggc tttgttaata tcaactgttg ttaagccagt tacttagttg tcatcttaca
    19381 agaaggagaa ggtcttgtag ctaactgggc acatgccttc ccttcgtaat acacaggttc
    19441 tgattcggtt ccatgtccaa aggaatgtgg tggtgatccc caagtctgtg acaccctcac
    19501 gcatacagga gaacctgcag gtaagacctg ggctgtacat ctgacattcc tcagtggaac
    19561 aggtgatagg agggaaggag gactcttctt ccctgccctt cagcttctca tcccactctt
    19621 tcactgacag tgtgtcttcc cttcttccaa gcactctgtc aacttgtagg ctagaaagct
    19681 gggatgactg agctcacagg tggtaccggc caaacctgtc aggactgatc acaactgtag
    19741 cttatatcac tgagatgacc actgcctacc attgagcact tagatcatag gtggaatgct
    19801 gtgctagcca actgaagact gccttgtaga catggactgg acttgaaaac ttgtcctctg
    19861 agacaccctt caggcataca tacttagcac acctacaacc caatctcttc cctcttagaa
    19921 cactaaactc tataaataac tcactcttaa tttagagaat aatgtatttg tcatggacat
    19981 attaggggaa acacctcaga tccctccagc actttccttg ctaagggtgg ttttggaact
    20041 cttgtctttc taggtcttcg acttccagtt gagtgaggag gacatggctg ccattctcag
    20101 cttcaacagg aactggaggg cctgtgacct gttggagtaa gtggtaccag gttaagtagg
    20161 aggttgatta ggcctctccc taagggtaaa gggttagtag gaagggatgg aaggcatgtg
    20221 ggattatggg atggtttttg tcttcaaata ctattttctg gcagttgtga aaagttaaaa
    20281 agaaaagtct gagtcctggg gactcactaa gggcagagac attgtcatct gatgctcagt
    20341 ttctgaatct tacacacaga gtgaggattt taaagaaagg agctgtcact gctgtgtggg
    20401 agtcaagttc tccttccctc actcattcac ccagtgactt cctgtaatgt gctatggcca
    20461 ttcatggtct acatgtttgg gacatcacac tataagcttc ctgttcccaa gctcatattc
    20521 tacagaggac aactgaagta actctgatct cagttacata caccaatcag agagtgacaa
    20581 ataccatata gaaaagtgaa gcaggacaaa aggtatagct gccctttgta atagacactt
    20641 taatacatga ctatccaggg gaagctttgt tcaaaaagtg gcagttgaag acagtggaag
    20701 atgagtaaca tggctattta gggaaatagc attcttgcaa tcatagcaac cattgcagat
    20761 gctctgctct cgtagctaga gcatttgaat gggattgaga atactgtgga atgaataagt
    20821 gttattgtaa gaacgcagaa acttgaggag atttaggtta aggctatgga caggcctttg
    20881 atttttaggc ctgaaagagg aataagccat gaacagagct ggtatgatcc gatgtgtctt
    20941 aaaaggctca caatgacttg tgggttgaga gactacgaca gtaacccatg gaaaagatga
    21001 cagtcgctgg acctggacca tggtgttggg gaggagatga ggaagaccac catctagata
    21061 aatgggaaca tgcaatcaat ttagtttcct cctcaaaatt tacagttgaa gctggttttg
    21121 tatttttgtt tgcgtggttg gcttttgttt gagcaactca catgcagaat taacattttg
    21181 gtaaaatcaa gggtttgaca tgcgtttatt atgcctgttg ggtatcaact aggagtatat
    21241 gtgaagctag tgaatttggt gacctcagaa gccaactgtc actgggttct aatccaaaat
    21301 ctactactta ttcactagca ctggggccct tggggagttt tggtgatgtt ctaaaatcac
    21361 ttttctaatc tacaaagtaa ggatgacaat attaatcaca gccaaccttc actgaattct
    21421 ctcgtttagc atgcagcaga cactttaagc cctttgctta tattagtatc atatttacta
    21481 ctcataccca atctattgat ttcaatagta tttcctgctt cacagcaatg ccagagtttg
    21541 gacaatagtt ctggggccct tagttgtgtg tctgcttgtt tatgatccct taagatatca
    21601 atgaactggt gaccgagtga ctgatagctc gcaggccttc cttccttaat actcctctga
    21661 tattttggga gaaggtcctg ttgtgtgccc gcgccacact ccatgtccca tgttcacagt
    21721 cttgttgggg aagagatcag aagagtacag aaatagctga atatatcacg taagagtgtc
    21781 tcagatcaca tttttgattc tgtggactgt tttcttcttt gtatttgttg ttgcagtgca
    21841 aggactgaag aggactatcc tttccacgag gaatactgag gtccacttgc ttgatgagat
    21901 ccgtgcatga tagattcctt cgtctctcta aaatcaacct ggatgtaggc taccatctgg
    21961 caatgcagga tttaaatttt tgtgttcatc aaggagcaag aaaatgttcc agagaaacga
    22021 agggcaacgg ttctcactag cgagggctga gcacatgttt gtcatcagga gcatgtagta
    22081 tggtaacact gaagatacaa tgatagaaca ataaaaaagc aataatagta attcctgtag
    22141 tatgctgtcc atttctgtga taaccactat gaccaaaagc aacttgtaga ggaaagggct
    22201 tatttgtctt acatgtttca tccgtagtcc atcacagaag gaagccatag cagaaacaga
    22261 ggcaggagct gggtcgctga agcatagctc atggaggaat gctgctcagt ggattgttcc
    22321 atggcttatc cagctttctt ttcttttctt tttctttttt tctttttttt tttttttttt
    22381 ggtctttttt gagacagggt ttttctgtat agccctggct gtcctggaac tcactttgta
    22441 gaccaggctg gtctcgaact cagaaatccg cctgcctctg cctcccaagt gctgggatta
    22501 aaggcgtgca ccaccacgcc tggctttcag cttgctttct tatacaaccc agcaccactt
    22561 gcccaggggt ggcatcaccc acacaggact gggctctcct agaccaataa atcactaatt
    22621 aagaaaatcc cctacagact tgtccacagg ccatctgatg gagtcaattc ctcaactgag
    22681 gttctctctt cctaggtgac ttcagtttgt gtcaaggtga caaaaactaa tgagcatggt
    22741 caccaaagca tctgacacaa tttgaaatac tttc
//