LOCUS       92080#pL1L2GT0_LF2A_nEGFPOT2A_CreERT2_pAbActNeopA#L3L4_pD223_DTA_T_spec        20720 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2GT0_LF2A_nEGFPOT2A_CreERT2_pAbActNeopA
COMMENT     design_id: 92080
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000035085
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            6267..6356
                     /db_xref="ENSEMBL:ENSMUSE00001238402"
                     /rank="5"
                     /note="ENSMUSE00001238402"
     exon            5541..5582
                     /db_xref="ENSEMBL:ENSMUSE00001283030"
                     /rank="4"
                     /note="ENSMUSE00001283030"
     misc_feature    4275..10201
                     /note="5 arm"
     misc_feature    10229..10253
                     /note="B1 Gateway"
     misc_feature    11220..11223
                     /note="FCHK QC critical region"
     misc_feature    11204..11219
                     /note="FCHK QC prefix region"
     misc_feature    11224..11229
                     /note="FCHK QC suffix region"
     misc_feature    11352..11403
                     /note="F2A"
     misc_feature    11229..11351
                     /note="1Dleader"
     primer          11089..11110
                     /vntifkey="27"
                     /dnas_title="seq primer FCHK2"
                     /label=seq primer FCHK2
                     /note="seq primer FCHK2"
     exon            11189..11228
                     /vntifkey="61"
                     /dnas_title="mouse En2 exon"
                     /label=mouse En2 exon
                     /note="mouse En2 exon"
     misc_feature    12275..12275
                     /note=" A to G to kill RsRII site"
     misc_feature    12225..12278
                     /note="self-cleaving' 2A peptide"
     misc_feature    12279..13304
                     /note="Cre"
     misc_feature    11409..11498
                     /note="3X NLS"
     misc_feature    11499..12215
                     /note="EGFP"
     primer          complement(10382..10403)
                     /vntifkey="27"
                     /dnas_title="LRPCR LAR3_EN2"
                     /label=LRPCR LAR3_EN2
                     /note="LRPCR LAR3_EN2"
     intron          10314..11188
                     /vntifkey="21"
                     /dnas_title="mouse En2 intron"
                     /label=mouse En2 intron
                     /note="mouse En2 intron"
     primer          complement(10278..10301)
                     /vntifkey="27"
                     /dnas_title="FRT3_oligo"
                     /label=FRT3_oligo
                     /note="FRT3"
     misc_feature    10266..10313
                     /vntifkey="21"
                     /dnas_title="Frt"
                     /label=Frt
                     /note="Frt"
     misc_feature    14565..14572
                     /vntifkey="21"
                     /dnas_title="polylinker"
                     /label=polylinker
     misc_recomb     14533..14564
                     /note="Rox(inv)"
     misc_feature    14525..14532
                     /vntifkey="21"
                     /dnas_title="RE sites"
                     /label=RE sites
     primer          14465..14483
                     /vntifkey="27"
                     /dnas_title="seq primer NF"
                     /label=seq primer NF
     primer          14339..14362
                     /vntifkey="27"
                     /dnas_title="LRPCR RAF5"
                     /label=LRPCR RAF5
     misc_feature    14282..14524
                     /vntifkey="21"
                     /dnas_title="SV40 pA"
                     /label=SV40 pA
     misc_feature    13314..14258
                     /note="ERT2"
     misc_feature    15360..15942
                     /note="synthetic fragment mutates RsRII"
     misc_feature    15772..15772
                     /note="G to C conservative mutation to kill RsrII site"
     misc_feature    16120..16363
                     /note="SV40pA"
     misc_feature    16178..16201
                     /note="RAF5"
     misc_feature    15927..15946
                     /note="neoBstB1_5"
     misc_feature    15634..15652
                     /note="neoSphI_5"
     misc_feature    15297..15316
                     /note="neoPstI_5"
     misc_feature    15128..16113
                     /note="neomycin resistance "
     misc_feature    14978..14997
                     /note="betact5"
     promoter        14582..15102
                     /note="human beta actin promoter"
     insert          14279..16358
                     /source="pL1L2_GTIRES_BetactP FLFL MapVerified"
     misc_feature    16359..16363
                     /note="for bActNeo"
     misc_recomb     16365..16396
                     /note="Rox"
     misc_feature    16409..16429
                     /note="B2 Gateway"
     misc_feature    10202..16459
                     /note="Synthetic Cassette"
     exon            16857..17001
                     /db_xref="ENSEMBL:ENSMUSE00001303157"
                     /type="targeted"
                     /rank="6"
                     /note="target exon 1 ENSMUSE00001303157"
     misc_feature    16460..17156
                     /note="Critical Region"
     misc_feature    17180..17213
                     /note="Downstream LoxP"
     misc_feature    17157..17236
                     /note="synthetic loxP region"
     exon            19761..19901
                     /db_xref="ENSEMBL:ENSMUSE00001307046"
                     /rank="9"
                     /note="ENSMUSE00001307046"
     exon            19003..19062
                     /db_xref="ENSEMBL:ENSMUSE00001243829"
                     /rank="8"
                     /note="ENSMUSE00001243829"
     exon            18260..18338
                     /db_xref="ENSEMBL:ENSMUSE00001231048"
                     /rank="7"
                     /note="ENSMUSE00001231048"
     misc_feature    17237..20720
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggacctcta ggatgtgtct gaggttgcat taggagtttg gtcgttaaag
     4321 gattcggaga tcaagtttaa gaatcgaccc caattgttca ggtttttctt cttcctcctc
     4381 ttcttcttct tctcttcttc ttcttcttct tcttcttctt cttcttcttc ttcttcttct
     4441 tcttcttctt cttcttcttc tccttctcct tctccttctc cttctccttc tccttctcct
     4501 tctccttctc cttctccttc tccttctttc tccttccttc tccttccttc tccttctttt
     4561 ggtttttcga tcgttttgct tttttaagga tgccataaaa gtgcctttag aaacaaataa
     4621 taaagtcatc caaggagcat atttaatcac tgcatttgta tacaggagcc cagtgctcct
     4681 tgatagacta cctcaagctg tgtgtgtaag gactttacag atgtggcttt aggctcttga
     4741 agtcttggca caaattcctt attacatgga aagatggaga gacagctcat acatgtcaaa
     4801 gccctgtatg ctagtcatcg tagatgtgtt aaggtacaaa tcttattttc aggcaaacgg
     4861 aaacccaaga gtcctcagct gaagagtcag aacacaaaga caaattgggg gaagggagtg
     4921 ggacctggaa agagctggct ggggaagcag gggagaggca gtggaaagag ctggctggtg
     4981 aaagcagggg agaggcagtg gaaagagctg gctggggaag caggagagag gcagtggaaa
     5041 gagctggctg gtgaaagcag gggagaggca gtggaaagag ctggctgggg aagcagggga
     5101 gaggcaggat taactacaag gaagaaaaga tgcgggtgca gggaaggcac actgagcttg
     5161 taaatgctat cgattttcat tgaactgatt cacagatgtg ttagccatct caatgagcca
     5221 accttatcaa aagccagtga acatagagtc atcagaaact ttccaggttg gttcttcatt
     5281 cctgggtttg ctaggaggac aaaggactaa aagtgacagt gtcgaatgat tgggacttag
     5341 gccaggaggg tgttctgcct agtggccgta acatctttgc ttcaaccaag gatatcccat
     5401 tatcaacgat aatgacatgt tcccctttga tttttctctc cccacccctc cttctgtctt
     5461 tctctccatc tctttctggt tctctgggtt tttttgtttt ttgttttgtt tctgtttctg
     5521 tttctgcttc taccatgcag cggcgactgg cggctcagaa ccaagagagg agaaaatcca
     5581 aggtagggat ctccagggtc ccaggggagt cagcaccaca gttagaataa acaatacatt
     5641 tcccatcaga gctgaagatg tgcccatatc tcagaggctg gggtgggtcc tgcctgtccc
     5701 atacaagttg gctgtcatgc tcaccttctg tctcatgtgg atgcttctgg ctcagaatct
     5761 gaacatcctt aagtgttcct aattacagta ggagtttctg tggtgcttga cccaatccac
     5821 atccactgga gattttctct tgttgttgtt gttggctaaa atttagagca gacatttcaa
     5881 tctttcaagt gatctatacc tgaaaatgaa gagacggtgt tgctcagtgc ttagaatgcc
     5941 tccgctgtcg aatcttctcc taatttagtt ttaaaggaaa tttgccctgt cagggctttc
     6001 ttgtgatgtt ttcaagaaat gacattcatt caccctttat gcttgagcca agatcgttaa
     6061 gaactgttgc aagagttttt atgctgggca ctggctctct gatacttctc tttacccttt
     6121 gcacccagaa gcatttagca cattatatcc tggactgaga agtctgattt gggaatattc
     6181 atgcaaataa tgtgatcgac cgccatgctt tgacagtctg agaatgcttt ctgctttttt
     6241 tccccctcct taatctcaat ctgcagtcag gagcaggcaa agggaagctg accagaagtc
     6301 ttgctgtctg tgaagagtct tcagctagat ctggagggga aagtcaccag gatcaggtat
     6361 aaaagctggt actagaaggg attgcatgat gacatctctg tgtgcatgct caggtataaa
     6421 cctggtatta gatgggattg catgacggca atgcatggcc actactaatt tgacattata
     6481 atgatgtttt gcgggagaaa acaggaattg aagaacgagc attttcttgc ttcctcctgt
     6541 ttatgagaca ttctgcatag tttagaagtt tggggctggg gacataactt agcaagttaa
     6601 cggcttgcta cacaagcagt ttggcctatg tttaagccct ggaacccact caaaaaatga
     6661 acatggtgca tatacttgta atctcagcac cagggaaata aagacagaca gattgactcc
     6721 tgggattcaa gggccagttg gctcaaggcc taaccagtga gtcccaggtc cccgtgagag
     6781 actgtctcaa aaataaataa atgaataaat aaataaataa ataaataaag gcagatagca
     6841 accgaggaga aacacctgag actgacgtct ggctccacat gcacaggaac ttgtactccc
     6901 agatgtacct cccacaaata tgcaaataac ccccccacac actcatacac ccccacaccc
     6961 ccacactccc catatcttgc acaactagtt tcaatgtata tcacaggata ttgcatttgt
     7021 ttttttccag actctctgag aactgcaaat ggaaaggaac tcaaaagaat ttagattaaa
     7081 acttaactta aaataaaata aaataaaata aaaatccagt aagcacggta gtgctggata
     7141 tccaccaagg ctctctcttg ataaggctga accaaataga acccaagcat cctgtctttc
     7201 tggagacagc ttacctctca gctcccaaat gaacttgcct ctaagtaaaa caccactgtt
     7261 ggctcctagg ggatgtaaga agtagtgtct aagcaccctg gactgtggta aagtgccgga
     7321 atggacgtca ggagcttttt atttttattt tatttttttg actactgcaa aaacggtccg
     7381 gaaaagcatc tgacctggtg gcaaatttga tattagccag ctgctacata tctccaccct
     7441 tatctttgat tcccaaaact taaaattcca agaaccttca caccctcttg ttactctttt
     7501 tttttccttt ttaactatta tatttatctg cgtatggaaa acatagtctt gatgctgaaa
     7561 ttttgcttat ttttctcaga ggtcaaaggc agccaagaca aaaggcagat gatctgtctg
     7621 agcccagagg aagggaaatt gattacaaaa tcattcattt ttgtttggct ttctttggct
     7681 tctcttttat cttcccaccc ccctttctgc tttaattgaa atcctgtaat tctccttatg
     7741 gacacagaga acatggagag tgctttcttt aaaaaagaac caaaagaaaa aaaaatcctt
     7801 atagatgttt gtcctaggag tgtttccttt attagtctca ttttcttcac atgccgttca
     7861 tgtggagagg cacagacaag ctctcgtttg gtagccatta ctatgtgtca tagacagccc
     7921 cctaccccct tttttaaaac ttgggctctg agaaacgtgt ggctttcctt cagcatttta
     7981 tgtgtgcttc tatttttaac ggagatggga aaggtggaat ggtgtgaatc ccatgccttg
     8041 tactgtcaat gtagatgggt ggcttgtgat gtacagcatc tgacttgtgg gaaaccctgt
     8101 acagtgccag gctccgggag aagtaaggac ccagtcgtcc ctcactccta atgccaagca
     8161 ggggaggcca gcctctgtag atttcatttg ctctttgtct cttttgacaa ggcacaggag
     8221 tcttggtaag agccatctgt gccatagcac ccatttcaga ggcaggtcta gtggtacctc
     8281 cacacagtgg gtgaaaacgg tgctttgttc ctgctggttc tgaacccaga atacaggatt
     8341 catgttggag cagagaatta ggcccctcca gctttctgtg gactagccaa agagtggtgg
     8401 gaataggggg ttggcagaaa accaacagac acatatataa tcaactcacc cgatcttaag
     8461 aatttatacg caatgccttc actagataac actgaacact tagaaaatgt gtggactgac
     8521 tcccagagaa gtatacagta catggaatgt attgcttccc ccgtgtttgt atattcacag
     8581 tatttgggaa actgccttca ttttcctgtg tgggaaaagc tttcttgtcc cccatgttac
     8641 ttgatctcag acccacacct gatggttcag tctgtcttcg agtaaaggaa gcgtgttttt
     8701 ttttctaatg ttataatatt tactgaccag tgtgttactg caacttgaat cattctttta
     8761 ctgttgttgg gtataaactt atcctgtacc aatgtattta ttaacacttg tattttaata
     8821 ttgagcgtat caataaaaat atttaaaaat aacagattgt tttttaccaa ttctacagca
     8881 ctttttgtgt attctttcta caccatattg tatatttatg tgtagaaata tatttttttg
     8941 aacctgagta tagtatctgt caacacacag cagcaatttg tacattcggc tacagtgggt
     9001 gtctttggct tctgtttttg tatataggat ttattgccaa catgatgact tttttagatt
     9061 ccaacttaga gagggctgga tggttcgtac agttttgaag aagaaatgga accattctct
     9121 catgacccac gtgcccacaa tttctctatg agtctactga tggcctggct gttcctcaga
     9181 taccacctca gctggaaatg tcctagggat gtcctgagaa agtaaaagca aactccagcc
     9241 tctagctcct ccttgaaccc tcactgactg caacagttct ctgtccctaa tcacttgaag
     9301 aatgactaca gtagaattac agtctgattt gcatatcttg tatatgtcca cccctccccc
     9361 caaaataacc ccccaggagt tggaaaaccc aacttagtta aaaatttagg aatgacagca
     9421 tgttttatct cctcttccat atcgtggaag ttggagatct tttttgtttt gttttgcttt
     9481 ggtttggttt agtttttgtt tgtttgtttt ctctcaccac cgtggcaact tcccttcagc
     9541 tatcatttct aatgggggtc cctcctcatt ggaaacaaga ctcaccccct gaccaatagt
     9601 cgctgaagca gtgagtgtca ctaagtggtc tctcatatac cacacacaca aagccagccg
     9661 caacttccct aaaacatcat gactccaatc aacatacact gcagaaacta aaaaagccaa
     9721 acaaaacatc atgtgatgca ttctgctcca aagaagcaaa tgtgcaatca aagtaatggc
     9781 tactgcgtgt caatcaatat aagcagatca gtttccagga aaaaaaaaaa aaaaaaaaaa
     9841 aaaaaaaaca atgattagca atgtagatgt agttcaacac tgtagtctgt atcaagaagc
     9901 agcacaggag aatctgtttg gggtaaaata aactacattt accccaacat agacttatag
     9961 ataacttatg aaaacatgac aggactcacc acactttcca aaaggctatg cctttttgtg
    10021 actctgattt ataacaaaat attaatgaca ttcataaacc agccctataa ccccagatct
    10081 aagatctcag cagttacagg gttcagttcg ccttgtgtgg ggtatccttc gtggcctggt
    10141 tccatggtag gatttctact gtaaattgga tgttgggtgc tttctttctc cgagttctaa
    10201 caaggcgcat aacgatacca cgatatcaac aagtttgtac aaaaaagcag gctggcgccg
    10261 gaaccgaagt tcctattccg aagttcctat tctctagaaa gtataggaac ttcgaaccct
    10321 ttcccacacc accctccaca cttgccccaa acactgccaa ctatgtagga ggaaggggtt
    10381 gggactaaca gaagaacccg ttgtggggaa gctgttggga gggtcacttt atgttcttgc
    10441 ccaaggtcag ttgggtggcc tgcttctgat gaggtggtcc caaggtctgg ggtagaaggt
    10501 gagagggaca ggccaccaag gtcagccccc cccccctatc ccataggagc caggtccctc
    10561 tcctggacag gaagactgaa ggggagatgc cagagactca gtgaagcctg gggtacccta
    10621 ttggagtcct tcaaggaaac aaacttggcc tcaccaggcc tcagccttgg ctcctcctgg
    10681 gaactctact gcccttggga tccccttgta gttgtgggtt acataggaag ggggacggga
    10741 ttccccttga ctggctagcc tactcttttc ttcagtcttc tccatctcct ctcacctgtc
    10801 tctcgaccct ttccctagga tagacttgga aaaagataag gggagaaaac aaatgcaaac
    10861 gaggccagaa agattttggc tgggcattcc ttccgctagc ttttattggg atcccctagt
    10921 ttgtgatagg ccttttagct acatctgcca atccatctca ttttcacaca cacacacacc
    10981 actttccttc tggtcagtgg gcacatgtcc agcctcaagt ttatatcacc acccccaatg
    11041 cccaacactt gtatggcctt gggcgggtca tccccccccc cacccccagt atctgcaacc
    11101 tcaagctagc ttgggtgcgt tggttgtgga taagtagcta gactccagca accagtaacc
    11161 tctgcccttt ctcctccatg acaaccaggc tcccaggtcc cgaaaaccaa agaagaagaa
    11221 cgcagatctc tagagtcacc gagttgcttt accggatgaa gagggccgaa acatactgtc
    11281 caaggccctt gctggcaatc cacccaactg aagccagaca caaacagaaa attgtggcac
    11341 cggtgaaaca gactttgaat tttgaccttc tcaagttggc gggagacgtc gagtccaacc
    11401 ccggaccggt gcatgtggac cccaagaaaa agcggaaggt ggaccctaaa aagaaacgca
    11461 aagtggatcc taagaaaaaa agaaaggtgc acgtgtccat ggtgtccaag ggcgaggaac
    11521 tgttcaccgg cgtggtgccc atcctggtgg aactggatgg cgacgtgaac ggccacaagt
    11581 tcagcgtgtc cggcgagggc gaaggcgacg ccacatacgg aaagctgacc ctgaagttca
    11641 tctgcaccac cggcaagctg cccgtgcctt ggcctaccct cgtgaccaca ctgacctacg
    11701 gcgtgcagtg cttcagcaga taccccgacc atatgaagca gcacgacttc ttcaagagcg
    11761 ccatgcccga gggctacgtg caggaaagaa ccatcttctt taaggacgac ggcaactaca
    11821 agaccagggc cgaagtgaag ttcgagggcg acaccctcgt gaacagaatc gagctgaagg
    11881 gcatcgactt caaagaggac ggcaacatcc tgggccacaa gctggagtac aactacaaca
    11941 gccacaacgt gtacatcatg gccgacaagc agaaaaacgg catcaaagtg aacttcaaga
    12001 tccggcacaa catcgaggac ggctccgtgc agctggccga ccactaccag cagaacaccc
    12061 ctatcggcga cggccctgtg ctgctgcctg acaaccacta cctgagcacc cagagcgccc
    12121 tgagcaagga ccccaacgag aagagggacc acatggtgct gctggaattc gtgaccgccg
    12181 ctggcatcac cctgggcatg gacgagctgt acaagcctgc aggcgagggc agaggaagtc
    12241 ttctaacatg cggtgacgtg gaggagaatc ccgggccgtc caatttactg accgtacacc
    12301 aaaatttgcc tgcattaccg gtcgatgcaa cgagtgatga ggttcgcaag aacctgatgg
    12361 acatgttcag ggatcgccag gcgttttctg agcatacctg gaaaatgctt ctgtccgttt
    12421 gccggtcgtg ggcggcatgg tgcaagttga ataaccggaa atggtttccc gcagaacctg
    12481 aagatgttcg cgattatctt ctatatcttc aggcgcgcgg tctggcagta aaaactatcc
    12541 agcaacattt gggccagcta aacatgcttc atcgtcggtc tgggctgcca cgaccaagtg
    12601 acagcaatgc tgtttcactg gttatgcggc ggatccgaaa agaaaacgtt gatgccggtg
    12661 aacgtgcaaa acaggctcta gcgttcgaac gcactgattt cgaccaggtt cgttcactca
    12721 tggaaaatag cgaccgctgc caggatataa ggaatctggc atttctgggg attgcttata
    12781 acaccctgtt acgtatagcc gaaattgcca ggatcagggt taaagatatc tcacgtactg
    12841 acggtgggag aatgttaatc catattggca gaacgaaaac gctggttagc accgcaggtg
    12901 tagagaaggc acttagcctg ggggtaacta aactggtcga gcgatggatt tccgtctctg
    12961 gtgtagctga tgatccgaat aactacctgt tttgccgggt cagaaaaaat ggtgttgccg
    13021 cgccatctgc caccagccag ctatcaactc gcgccctgga agggattttt gaagcaactc
    13081 atcgattgat ttacggcgct aaggatgact ctggtcagag atacctggcc tggtctggac
    13141 acagtgcccg tgtcggagcc gcgcgagata tggcccgcgc tggagtttca ataccggaga
    13201 tcatgcaagc tggtggctgg accaatgtaa atattgtcat gaactatatc cgtaacctgg
    13261 atagtgaaac aggggcaatg gtgcgcctgc tggaagatgg cgatctcgag ccatctgctg
    13321 gagacatgag agctgccaac ctttggccaa gcccgctcat gatcaaacgc tctaagaaga
    13381 acagcctggc cttgtccctg acggccgacc agatggtcag tgccttgttg gatgctgagc
    13441 cccccatact ctattccgag tatgatccta ccagaccctt cagtgaagct tcgatgatgg
    13501 gcttactgac caacctggca gacagggagc tggttcacat gatcaactgg gcgaagaggg
    13561 tgccaggctt tgtggatttg accctccatg atcaggtcca ccttctagaa tgtgcctggc
    13621 tagagatcct gatgattggt ctcgtctggc gctccatgga gcacccagtg aagctactgt
    13681 ttgctcctaa cttgctcttg gacaggaacc agggaaaatg tgtagagggc atggtggaga
    13741 tcttcgacat gctgctggct acatcatctc ggttccgcat gatgaatctg cagggagagg
    13801 agtttgtgtg cctcaaatct attattttgc ttaattctgg agtgtacaca tttctgtcca
    13861 gcaccctgaa gtctctggaa gagaaggacc atatccaccg agtcctggac aagatcacag
    13921 acactttgat ccacctgatg gccaaggcag gcctgaccct gcagcagcag caccagcggc
    13981 tggcccagct cctcctcatc ctctcccaca tcaggcacat gagtaacaaa ggcatggagc
    14041 atctgtacag catgaagtgc aagaacgtgg tgcccctcta tgacctgctg ctggaggcgg
    14101 cggacgccca ccgcctacat gcgcccacta gccgtggagg ggcatccgtg gaggagacgg
    14161 accaaagcca cttggccact gcgggctcta cttcatcgca ttccttgcaa aagtattaca
    14221 tcacggggga ggcagagggt ttccctgcca cagcttgatg aagatctgag ctcttaatta
    14281 atgatcataa tcagccatat cacatctgta gaggttttac ttgctttaaa aaacctccca
    14341 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
    14401 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
    14461 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
    14521 atccgtttaa actaacttta aataattggc attatttaaa gttacatatg gcggccaatt
    14581 cgccttctgc aggagcgtac agaacccagg gccctggcac ccgtgcagac cctggcccac
    14641 cccacctggg cgctcagtgc ccaagagatg tccacaccta ggatgtcccg cggtgggtgg
    14701 ggggcccgag agacgggcag gccgggggca ggcctggcca tgcggggccg aaccgggcac
    14761 tgcccagcgt gggcgcgggg gccacggcgc gcgcccccag cccccgggcc cagcacccca
    14821 aggcggccaa cgccaaaact ctccctcctc ctcttcctca atctcgctct cgctcttttt
    14881 ttttttcgca aaaggagggg agagggggta aaaaaatgct gcactgtgcg gcgaagccgg
    14941 tgagtgagcg gcgcggggcc aatcagcgtg cgccgttccg aaagttgcct tttatggctc
    15001 gagcggccgc ggcggcgccc tataaaaccc agcggcgcga cgcgccacca ccgccgagac
    15061 cgcgtccgcc ccgcgagcac agagcctcgc ctttgccgat cctctagagt cgagatccgc
    15121 cgccaccatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
    15181 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
    15241 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
    15301 tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
    15361 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
    15421 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
    15481 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
    15541 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
    15601 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat
    15661 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
    15721 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg ccgaccgcta
    15781 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
    15841 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
    15901 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg
    15961 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
    16021 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
    16081 ttcttcgccc accccccgga tctaagctct agataagtaa tgatcataat cagccatatc
    16141 acatctgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
    16201 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
    16261 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
    16321 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccctaactt taaataatgc
    16381 caattattta aagttaggaa ttaattcacc cagctttctt gtacaaagtg gttgatatct
    16441 ctatagtcgc agtaggcgga agcctttttt agacttgaca gtggctccaa tatgaacaga
    16501 ttaaaggatc ctccctacac acactacaca tttaaaaata aacctgtagg gaaaaataag
    16561 agttgtgatc tacaggagtt tagcttgatt aaagttaata ttcatgaggc aaagtttaat
    16621 actagatatg tcttctgacc tcccattgac ttctggattt cagttcaccg taaagggcaa
    16681 gtattctttt tttttttttt ttttttcctg acaggcttaa ttttttaaac cacttaagaa
    16741 agaggaatcc atacgaacct ctcatttttc aatacctcgt aacctctgtg cagaatggaa
    16801 gccaggcagg gatgattact tctggcccta aattatgata ttttttcccc caacaggaat
    16861 caattcactt acagctttcc agtttcccca gcctgcaaga ggaggataaa tctaggaagg
    16921 atgattctga gagagaaaaa gaaaaggata agaacagaga gaaactctct gagagaccca
    16981 agatcagaat gttatcaaaa ggtgaatctg caaaacattt ccttaccttg cttatcatgg
    17041 acactcgcaa agccaagaga cattgcaaac ccacaccaat gtttacagcc cagggcactc
    17101 tgcacctaat tttccaagtg gccctatatg tgtaagagag aaaaaaatag cgacctgaga
    17161 tggcgcaacg caattaatga taacttcgta tagcatacat tatacgaagt tatggtctga
    17221 gctcgccatc agttcaggag aaagagggaa ttgaaatcgg ttcctgtatt caggttcact
    17281 gacaactgac ttggattggc gaacaatggt gatacacagc tttctatttt cgctctcacc
    17341 cttatcttaa aagacaggca gcaagggcct tagcacctcc tcgtaataag ccacaccggg
    17401 tggaggtcaa tagaagccct ggcaccaagg gcttcagtct ctgcatctgg ccactgctgc
    17461 atgttgtttc atttgaaaac ataaatcaca tttctatttg gaaaagagcc attcctccta
    17521 aaaactatag acctacagtc cagattttta gactgtgaat gtacctctaa ttgtacaaag
    17581 tagaaaaaag ccaagctgga atggagagct atactggaga ccacactggg aatccaagca
    17641 gcctaggaat ggtagccact ctttgtgctc tgtaaacata ttcctgcttg gagcagttct
    17701 gtctagttga aaccctcact caagcttggg gtgtgtaaga ggaaagagca caacatgtct
    17761 aaaatgactg tataaacctg tatttatcca ggaccaggat ataagaaaaa ctcaagaaca
    17821 tttatctctt gccatgtttt atactgatta atatttaggt agttttataa tcaaaacttc
    17881 atttttaaaa actgttctgt tttaatttaa aaaaaaaaaa ggttgtcatg ggatcacaag
    17941 ctcttgaggt ctctgtgggt tggatgtacc tgtcatgtgg aggcagtacc ccactggttt
    18001 tctgcacaga acacactcca ccaccaacag caagcacact tcccctcctg tgacactagg
    18061 gtcttcccac agtcaaggtc tcaaccctca agtcttattt ctgatgttat gatccctgct
    18121 tgcaagtatt ttctccctgg taccttcctt tgaaatttac ctgagctgga aggaaaccag
    18181 aacgttttca aaccctgccc caccctgtat tcccatcatc catctgggca actcaactcc
    18241 ctcctgctgt ttgtttcaga ttgcagccaa gaatacacag attctacagg catagactta
    18301 catgggtttc taattaacac gctgaagaac aattccaggt aaatttaaat ggtgaaacaa
    18361 gctttttgct ggtgttgtgg gagggtgggg agtcagaaag aatattacaa aataattatg
    18421 attaatcaga atacttttaa gaaatcaaat actaaaaaca gatggcttcc ttgatgtctc
    18481 tcctgagctt gctctgctct gttgattgta tctccttgtg gatggctagc cctaatagtc
    18541 acatagttgc tccctgtata agttagagtg taccaacttt gctttgctaa ccccaggggc
    18601 atattgaagt aaaaaggaga gaatgaaatg atgtttattt acatcagtcc atggtgcctg
    18661 ggtgcaggca catagtcatg tgtactcagg gctccagctt ggcttctttc cagtttcttt
    18721 caactttaag tctttgttgg ttgtattatt atcattcaga aatttacata tcatgattat
    18781 cattcgaatg atgacattta acactaccaa atacttaaca agggagagga aaaatgaaac
    18841 ttctgagttc aaagtcgtgg catgcaaacc aaggagtcaa catagtccga agatgaatta
    18901 gagttaggtc tgctctgttt ccccgttgag tttgataaat tgtatttgct ctttgaaaac
    18961 taaaagtaca tgaaactttt cctttgaaca tttatttttc agggacagga tgatactctt
    19021 gaaaatggag caggaaatga ttgatttcat tgctgacagc aagtatgttc aatggcaatg
    19081 ctagctaatt cgatgacatg tcttagctca gtgtctgagt taatgactca ttccacagag
    19141 agctgtagat aaataaaatg tcagctcgta tggttcttgg tatgctcaat gaagaagtca
    19201 ctggatcttg gagtggtcat tgcttggatc acgtgtgatc ttgcctgtta aatttctggc
    19261 aaaatagcca taaacagggg gcttgcttag cccccactaa tttgcatgga ggagctgtaa
    19321 caagaaggga cccttctcgt ttttattata gttttttaat gggctccagc tggctgcagt
    19381 cagttatgcc tggaaataat tgtgctgcgt ttggtgtaaa ctgggtaatg acacatgtcc
    19441 agatagatat ctgcaatctc tattatgtca tggagcctgt cccctgcctg cctctgttga
    19501 taaggtatcc tgaactaacc cagaacactt tgcaacagac aaaatgaaca tgcggctgag
    19561 aaacagcaat gcctactgca gctcgatcaa taaatctatg atagtagaga tttgtctgcg
    19621 agagaagaca tcaggtggtg tcgaaatgaa agatgccaag tgtttctgga taaaaggaga
    19681 tagtgacgtg taaatccctg taaaatatac tactggcatg aaatgaagat gctgatttta
    19741 tttctgtaac tctgttccag taaccactat aaaaagttcc cccagatgtc atcctatcaa
    19801 aggatgctgg tccatcgggt ggcagcgtac tttggattgg accacaacgt ggatcaaact
    19861 ggaaaatctg tcatcatcaa caagaccagc agcaccagga tgtaagtccc aacgtagtgt
    19921 ctgtttggtg ttttgttttt ctgtcagaga ctggtttata agagcacact attcattgta
    19981 aagtatgcga cgttaaatat gtatagcgct ggttatcttt ctggcctaat agcagtggga
    20041 tgtcaggaat ccatataata ccaaatgaga tctagggaat ttggtgggag aacttcactt
    20101 caaaatagca ttcagattaa aaacaacagg cattgattgg tctgggagtg tagctcagtg
    20161 gtagagaaca tggatggcac acacaaagtt tattagattc ccagtgcaac aaatgggtaa
    20221 agcctacgca agcaagcaag caagcaagca agcaggcagg caggcaggca ggcaggcagg
    20281 caagctcttt ctgcagagac aggcatgata gcatatccct gtgctcccag tactctggac
    20341 actcaggcag gaggatcagg agttgaaaaa tagtcctata tacatgctat gattctgtca
    20401 aaaagacggg gaggggagat gaagggaggg tggatcaagg aggggatggg ttaggggagg
    20461 aggaatcaag gataggattt ataaagctgt tacacagaga catttgtcct tttctacccc
    20521 accccccttt ttgatgtggg ggagacatgt gttttgggga aagctcagaa caggatacag
    20581 aaaccttgct tctcaatcct ggcatactga ctgggcattc cagtctgctg cagtcctgat
    20641 ttcccatctt ggtttcttca cctgtgagac agataagttt tagccctaag aagacaacac
    20701 ttggtttttc tcaagatcat
//