LOCUS       39522#pL1L2GT0_LF2A_nEGFPOT2A_CreERT2_pAbActNeopA#L3L4_pD223_DTA_T_spec        20464 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2GT0_LF2A_nEGFPOT2A_CreERT2_pAbActNeopA
COMMENT     design_id: 39522
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000025166
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     misc_feature    4275..9039
                     /note="5 arm"
     misc_feature    9067..9091
                     /note="B1 Gateway"
     misc_feature    10058..10061
                     /note="FCHK QC critical region"
     misc_feature    10042..10057
                     /note="FCHK QC prefix region"
     misc_feature    10062..10067
                     /note="FCHK QC suffix region"
     misc_feature    10190..10241
                     /note="F2A"
     misc_feature    10067..10189
                     /note="1Dleader"
     primer          9927..9948
                     /vntifkey="27"
                     /dnas_title="seq primer FCHK2"
                     /label=seq primer FCHK2
                     /note="seq primer FCHK2"
     exon            10027..10066
                     /vntifkey="61"
                     /dnas_title="mouse En2 exon"
                     /label=mouse En2 exon
                     /note="mouse En2 exon"
     misc_feature    11113..11113
                     /note=" A to G to kill RsRII site"
     misc_feature    11063..11116
                     /note="self-cleaving' 2A peptide"
     misc_feature    11117..12142
                     /note="Cre"
     misc_feature    10247..10336
                     /note="3X NLS"
     misc_feature    10337..11053
                     /note="EGFP"
     primer          complement(9220..9241)
                     /vntifkey="27"
                     /dnas_title="LRPCR LAR3_EN2"
                     /label=LRPCR LAR3_EN2
                     /note="LRPCR LAR3_EN2"
     intron          9152..10026
                     /vntifkey="21"
                     /dnas_title="mouse En2 intron"
                     /label=mouse En2 intron
                     /note="mouse En2 intron"
     primer          complement(9116..9139)
                     /vntifkey="27"
                     /dnas_title="FRT3_oligo"
                     /label=FRT3_oligo
                     /note="FRT3"
     misc_feature    9104..9151
                     /vntifkey="21"
                     /dnas_title="Frt"
                     /label=Frt
                     /note="Frt"
     misc_feature    13403..13410
                     /vntifkey="21"
                     /dnas_title="polylinker"
                     /label=polylinker
     misc_recomb     13371..13402
                     /note="Rox(inv)"
     misc_feature    13363..13370
                     /vntifkey="21"
                     /dnas_title="RE sites"
                     /label=RE sites
     primer          13303..13321
                     /vntifkey="27"
                     /dnas_title="seq primer NF"
                     /label=seq primer NF
     primer          13177..13200
                     /vntifkey="27"
                     /dnas_title="LRPCR RAF5"
                     /label=LRPCR RAF5
     misc_feature    13120..13362
                     /vntifkey="21"
                     /dnas_title="SV40 pA"
                     /label=SV40 pA
     misc_feature    12152..13096
                     /note="ERT2"
     misc_feature    14198..14780
                     /note="synthetic fragment mutates RsRII"
     misc_feature    14610..14610
                     /note="G to C conservative mutation to kill RsrII site"
     misc_feature    14958..15201
                     /note="SV40pA"
     misc_feature    15016..15039
                     /note="RAF5"
     misc_feature    14765..14784
                     /note="neoBstB1_5"
     misc_feature    14472..14490
                     /note="neoSphI_5"
     misc_feature    14135..14154
                     /note="neoPstI_5"
     misc_feature    13966..14951
                     /note="neomycin resistance "
     misc_feature    13816..13835
                     /note="betact5"
     promoter        13420..13940
                     /note="human beta actin promoter"
     insert          13117..15196
                     /source="pL1L2_GTIRES_BetactP FLFL MapVerified"
     misc_feature    15197..15201
                     /note="for bActNeo"
     misc_recomb     15203..15234
                     /note="Rox"
     misc_feature    15247..15267
                     /note="B2 Gateway"
     misc_feature    9040..15297
                     /note="Synthetic Cassette"
     exon            15690..15801
                     /db_xref="ENSEMBL:ENSMUSE00000350907"
                     /type="targeted"
                     /rank="2"
                     /note="target exon 1 ENSMUSE00000350907"
     misc_feature    15298..15985
                     /note="Critical Region"
     misc_feature    16009..16042
                     /note="Downstream LoxP"
     misc_feature    15986..16065
                     /note="synthetic loxP region"
     misc_feature    16066..20464
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggatgttga taacatttat ccattcttca ttcccagccc tgatttaagt
     4321 ctgcatgcta acagggtctc tccagctgtc actcctcacc caaccccatt tcccatgaag
     4381 cagtctggag aatgccctat caaatacagt ttgctggtgt gttcctggtc acaaaacatc
     4441 ccccttgctc taattggtag gataacccca ggctcccaga catctatgat ttgaccatgt
     4501 gttactcccc agcttcaact tttgatttcc tgctgcatct tgggtcacac gacagggagc
     4561 tcattgtcct tctgcaggca tgtgctgttt cttgctgtct cattttagct cagacagtga
     4621 gtgagctcct gccacagttc ctcagcaggc ccctctgctt tgctactaca agcctctttt
     4681 ttgggagacc tcaggacccc ttcctcggag tttcatatcg ataccctggg gccaaacaca
     4741 tctacacttt ttttttgtgc tcttgagcct cattcatttt ccagtgacaa atgaacactc
     4801 agaaccagct tttccaactg atggttagtt gttttgtttt gttttgtttt tttaattgga
     4861 aatgcagtgg cattagaact ctggtggaag tgtggtcttg ggtatttcac ttcctgtgag
     4921 aaccaatttg ctcttctgta aattcctggt attcttccca ccccagagtc ttaaggataa
     4981 tcaactgagt aaccagtgtt ggctaaaact taagttattc caaggatctg caacagatag
     5041 ttctgtgctt ttgtgtggtc agaaacaatt cctacatctt tcagtgtgct tatgttgaaa
     5101 atcaaggttc actgggatta gtgtagtgtt gtttatttgt ttgccaaggg tttggctgct
     5161 tctgagtttc agcagcagag ttgagaacat gcaagagatc agcagacctc aaaaacttac
     5221 tggttgacct atatagaaaa aaagcttgct tacccatgag catgtacata cagattatgg
     5281 gaagttccca gctcatttca ggtacatggc aaacttcctt tctctgcatc taatttccag
     5341 aatttcatcc aggtatggaa taagggaggt gtcacaacaa aactttaatg ggctaattga
     5401 gaccatgggc ttcatacatg ggagtatgaa ggaaaggagt acaccccttt gttgtgtgtt
     5461 gtcatataac atacaactct ggctttggga gcatttgagt taaatacatc atggtgaatc
     5521 ctgttgtggg tgtgacactg tttgttagca tctctgatgt ctctatttct agaggctagc
     5581 attgcccctc caaactgtga caagctaaac tatgaggcat tgttgtgtgt tcaagggaca
     5641 aaaacacttg tggttaggaa acattaatgt agaacagtcc aacattgagt gtgggttgca
     5701 ctagagaacc ttccatatcc aagagctgtt tttgttagtg caagagtctt cagaagtaga
     5761 attaccttgc ctgggtagat ccccttgcca acaagctagt ctctgcaaat ctcagacctg
     5821 ataccagccc agcatgagtc tgggagaatg tagttgaacg gggaatttac taccctagta
     5881 cttgaaataa agtaaagaca aaaatctctc tagaagatta tatttacttg agtgctcagt
     5941 gtttatttaa catattgatt ttgttagttt gaaaaggaaa aaaagatgta tgtcacagat
     6001 atatacatat atacattcat gtatacatac atacgagtat ttataagtat atattatata
     6061 gcccaggcta gcctcaaatt tgctatgtag ttgagagtga ctcctgacct tcctgtgtct
     6121 gcctcccaag tccagagatt atggtcacac actaccatgt ccaatttgaa atagatacag
     6181 tgatagaaat atctacatgg tttagtaaca gcatgccttt tgcggatcct ttttgtctta
     6241 gggaagaata attaagtagt tgttggaagt ggacataaaa tccttccgtt aacacagggc
     6301 tcccctagtg tctgtcatga gcagtaaggg ctgtgaggga tgctctttat tactgctgtg
     6361 ttgtcattct catgggcaga tgaggcaagc agacatcctg gcatagcaga agaccaggaa
     6421 aagggcagca gagcgaaatg cttttctttg atgtaaagga gactaaacaa ttagaagata
     6481 acatccccct tatcctgatg accttgaagg agcctgtgag attggacgag agtattattg
     6541 gtgccagttc ttctacatag gtaaacatat gaattttaga gttgaagcaa aacactcatt
     6601 tgtccacagt ctttagactt taccagtcat gaagagcttc tctcttctac tgtgtacaca
     6661 cacacacaca cacacacaca cacacacaca cacacacaca cacacaggat aaaggctcaa
     6721 atgggaatgt ccttgccttg tctttcacag gttgcttttg tctggtcacc cctccagttc
     6781 tgccatgtcc cttgacaaga acctgccaca gctccctcat ggttttaagt ttgtgctggg
     6841 ggagaagagg ctaatcccta acattgcaat gggatgcagg cttatggcac atctctcccc
     6901 ttaatatttc agactggaga gatctcttca tttgttttgg tgagctaagc tttcctttta
     6961 ccaaatatct tctctgtcgc aggatatatg taactgcgat ctacaggagt atagtactat
     7021 aataccttct tgtatcacag actcaagtgt gggagacctc ccttcttact tctcacttgg
     7081 gactgagttg tgtctcttgt atcatgaaat tgatagcatg gggacatatt ggaaacaatg
     7141 caggctgtac ccacccacat gtccttacga gcctttccat cttaccttgc tgcttcacct
     7201 gctttacaga agacatgagt tactatggat aaatttgaag ccatctgtat gcattttttc
     7261 ctgttgcatg tcctagcctt cccttcctgg ggtaggctgc cattatttcc ttgtctctct
     7321 taaaattctc cttctatttg tatgggtcca tagtattgac tacctttgtg atgaaatttc
     7381 gtataaatgg aatcaccaat ctcattttta ttaattattt atttctaaga gagactccta
     7441 ctgccatgtg cttagccttc aggtttaacc ctgaatgcca cacaaatgtg ttttttactt
     7501 actcgaaata atgctagtgc tgagcttgat gtatgattaa atttctcccc ccacattgtg
     7561 tatgactata aggcactgat tcttgttgca tgttatgttg ttgtcctttc cacaagtttc
     7621 ctatgtggat acatgttgtc cagttcttct cttttaacat tgttggatat ttctcttttt
     7681 gagtgtgaga aaaggtattg gtccatttcc tattgatgga tgctctataa gtgcggaaac
     7741 agccatgctt cagtgaatat atatatatta gggcacccag gtgagggctt ctctgggaac
     7801 atagtgcagt attgaggaca gccatagtca ttctctacag actttgccga attgtacctc
     7861 cagtttctac acccactctt aggtaaagtg ctagggttct ctatccctga ttttatacgt
     7921 tctcttttca catatacttc acttgaacct gccatgaatg tgatgcattt tgtactgctt
     7981 ctcctttgtc tgtttgacta aaatacctga cggaagcgac tgggtggagg aaccattatg
     8041 tgggctcctg gttttctaag tgtctctcca tggcaattga tcagcttaca cttggacaga
     8101 gtggcatgca ggtgtcatct gtggcagagg ctggtcttca cagtagaaag gaagcaaaga
     8161 gaaaggaggg gtatacttgg ctgtctgttt cctttagctc atggaatggg gccacctaca
     8221 ttcagcctgc ttcttccccc tcagttgatc ccctttgtga atgtccttac agattcattc
     8281 aaaagtgtac cgcaatggtt ctctagttga ctctgtattt tgtctggatg acaatcaaaa
     8341 tctaccacca aaaagctaaa ctgcaatctc tctagttgct tcgaataata tttgcagatt
     8401 aaatactgtg cattgttgag tatatgttta cttttttatt tccttagacg tgaaaaaagc
     8461 agattagcta ttagatgagt ggctttttgc aacatcacta ttttcaaact tcatatgcgt
     8521 aattcatgga ttcttttaat taagaaaata aattgtattg cttcatccat cacctgtttg
     8581 gagttatgta tctttgggat tggggctggg aagcagtgat ggtttctttc tgatttgttg
     8641 tgttattaag gactattctt aacactctgt ctactggaca gttgatgagc tttgtggatt
     8701 cttcctactt ctgtgcttga ccattcatcc tgtagttaat aagaatagga aaagactaaa
     8761 catcctgaga ttggaccgat gaagccaggg gtagtagtgc ggatgttgat gataaattca
     8821 gcaccccttt gagtaactga gttttaagca ccctatgagt aactgtatat tgatacagac
     8881 aaagatgaaa aaatacgtat aactactttc ttaagtataa aaaaagaatc ttaattgaat
     8941 tgatttttat agttttaata tagccctatc ctttgaaata aagattatat atttgatatt
     9001 gtttggtttg acttagcatg tattatttat cctgcaatga aggcgcataa cgataccacg
     9061 atatcaacaa gtttgtacaa aaaagcaggc tggcgccgga accgaagttc ctattccgaa
     9121 gttcctattc tctagaaagt ataggaactt cgaacccttt cccacaccac cctccacact
     9181 tgccccaaac actgccaact atgtaggagg aaggggttgg gactaacaga agaacccgtt
     9241 gtggggaagc tgttgggagg gtcactttat gttcttgccc aaggtcagtt gggtggcctg
     9301 cttctgatga ggtggtccca aggtctgggg tagaaggtga gagggacagg ccaccaaggt
     9361 cagccccccc cccctatccc ataggagcca ggtccctctc ctggacagga agactgaagg
     9421 ggagatgcca gagactcagt gaagcctggg gtaccctatt ggagtccttc aaggaaacaa
     9481 acttggcctc accaggcctc agccttggct cctcctggga actctactgc ccttgggatc
     9541 cccttgtagt tgtgggttac ataggaaggg ggacgggatt ccccttgact ggctagccta
     9601 ctcttttctt cagtcttctc catctcctct cacctgtctc tcgacccttt ccctaggata
     9661 gacttggaaa aagataaggg gagaaaacaa atgcaaacga ggccagaaag attttggctg
     9721 ggcattcctt ccgctagctt ttattgggat cccctagttt gtgataggcc ttttagctac
     9781 atctgccaat ccatctcatt ttcacacaca cacacaccac tttccttctg gtcagtgggc
     9841 acatgtccag cctcaagttt atatcaccac ccccaatgcc caacacttgt atggccttgg
     9901 gcgggtcatc ccccccccca cccccagtat ctgcaacctc aagctagctt gggtgcgttg
     9961 gttgtggata agtagctaga ctccagcaac cagtaacctc tgccctttct cctccatgac
    10021 aaccaggctc ccaggtcccg aaaaccaaag aagaagaacg cagatctcta gagtcaccga
    10081 gttgctttac cggatgaaga gggccgaaac atactgtcca aggcccttgc tggcaatcca
    10141 cccaactgaa gccagacaca aacagaaaat tgtggcaccg gtgaaacaga ctttgaattt
    10201 tgaccttctc aagttggcgg gagacgtcga gtccaacccc ggaccggtgc atgtggaccc
    10261 caagaaaaag cggaaggtgg accctaaaaa gaaacgcaaa gtggatccta agaaaaaaag
    10321 aaaggtgcac gtgtccatgg tgtccaaggg cgaggaactg ttcaccggcg tggtgcccat
    10381 cctggtggaa ctggatggcg acgtgaacgg ccacaagttc agcgtgtccg gcgagggcga
    10441 aggcgacgcc acatacggaa agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
    10501 cgtgccttgg cctaccctcg tgaccacact gacctacggc gtgcagtgct tcagcagata
    10561 ccccgaccat atgaagcagc acgacttctt caagagcgcc atgcccgagg gctacgtgca
    10621 ggaaagaacc atcttcttta aggacgacgg caactacaag accagggccg aagtgaagtt
    10681 cgagggcgac accctcgtga acagaatcga gctgaagggc atcgacttca aagaggacgg
    10741 caacatcctg ggccacaagc tggagtacaa ctacaacagc cacaacgtgt acatcatggc
    10801 cgacaagcag aaaaacggca tcaaagtgaa cttcaagatc cggcacaaca tcgaggacgg
    10861 ctccgtgcag ctggccgacc actaccagca gaacacccct atcggcgacg gccctgtgct
    10921 gctgcctgac aaccactacc tgagcaccca gagcgccctg agcaaggacc ccaacgagaa
    10981 gagggaccac atggtgctgc tggaattcgt gaccgccgct ggcatcaccc tgggcatgga
    11041 cgagctgtac aagcctgcag gcgagggcag aggaagtctt ctaacatgcg gtgacgtgga
    11101 ggagaatccc gggccgtcca atttactgac cgtacaccaa aatttgcctg cattaccggt
    11161 cgatgcaacg agtgatgagg ttcgcaagaa cctgatggac atgttcaggg atcgccaggc
    11221 gttttctgag catacctgga aaatgcttct gtccgtttgc cggtcgtggg cggcatggtg
    11281 caagttgaat aaccggaaat ggtttcccgc agaacctgaa gatgttcgcg attatcttct
    11341 atatcttcag gcgcgcggtc tggcagtaaa aactatccag caacatttgg gccagctaaa
    11401 catgcttcat cgtcggtctg ggctgccacg accaagtgac agcaatgctg tttcactggt
    11461 tatgcggcgg atccgaaaag aaaacgttga tgccggtgaa cgtgcaaaac aggctctagc
    11521 gttcgaacgc actgatttcg accaggttcg ttcactcatg gaaaatagcg accgctgcca
    11581 ggatataagg aatctggcat ttctggggat tgcttataac accctgttac gtatagccga
    11641 aattgccagg atcagggtta aagatatctc acgtactgac ggtgggagaa tgttaatcca
    11701 tattggcaga acgaaaacgc tggttagcac cgcaggtgta gagaaggcac ttagcctggg
    11761 ggtaactaaa ctggtcgagc gatggatttc cgtctctggt gtagctgatg atccgaataa
    11821 ctacctgttt tgccgggtca gaaaaaatgg tgttgccgcg ccatctgcca ccagccagct
    11881 atcaactcgc gccctggaag ggatttttga agcaactcat cgattgattt acggcgctaa
    11941 ggatgactct ggtcagagat acctggcctg gtctggacac agtgcccgtg tcggagccgc
    12001 gcgagatatg gcccgcgctg gagtttcaat accggagatc atgcaagctg gtggctggac
    12061 caatgtaaat attgtcatga actatatccg taacctggat agtgaaacag gggcaatggt
    12121 gcgcctgctg gaagatggcg atctcgagcc atctgctgga gacatgagag ctgccaacct
    12181 ttggccaagc ccgctcatga tcaaacgctc taagaagaac agcctggcct tgtccctgac
    12241 ggccgaccag atggtcagtg ccttgttgga tgctgagccc cccatactct attccgagta
    12301 tgatcctacc agacccttca gtgaagcttc gatgatgggc ttactgacca acctggcaga
    12361 cagggagctg gttcacatga tcaactgggc gaagagggtg ccaggctttg tggatttgac
    12421 cctccatgat caggtccacc ttctagaatg tgcctggcta gagatcctga tgattggtct
    12481 cgtctggcgc tccatggagc acccagtgaa gctactgttt gctcctaact tgctcttgga
    12541 caggaaccag ggaaaatgtg tagagggcat ggtggagatc ttcgacatgc tgctggctac
    12601 atcatctcgg ttccgcatga tgaatctgca gggagaggag tttgtgtgcc tcaaatctat
    12661 tattttgctt aattctggag tgtacacatt tctgtccagc accctgaagt ctctggaaga
    12721 gaaggaccat atccaccgag tcctggacaa gatcacagac actttgatcc acctgatggc
    12781 caaggcaggc ctgaccctgc agcagcagca ccagcggctg gcccagctcc tcctcatcct
    12841 ctcccacatc aggcacatga gtaacaaagg catggagcat ctgtacagca tgaagtgcaa
    12901 gaacgtggtg cccctctatg acctgctgct ggaggcggcg gacgcccacc gcctacatgc
    12961 gcccactagc cgtggagggg catccgtgga ggagacggac caaagccact tggccactgc
    13021 gggctctact tcatcgcatt ccttgcaaaa gtattacatc acgggggagg cagagggttt
    13081 ccctgccaca gcttgatgaa gatctgagct cttaattaat gatcataatc agccatatca
    13141 catctgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
    13201 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
    13261 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
    13321 gtttgtccaa actcatcaat gtatcttatc atgtctggat ccgtttaaac taactttaaa
    13381 taattggcat tatttaaagt tacatatggc ggccaattcg ccttctgcag gagcgtacag
    13441 aacccagggc cctggcaccc gtgcagaccc tggcccaccc cacctgggcg ctcagtgccc
    13501 aagagatgtc cacacctagg atgtcccgcg gtgggtgggg ggcccgagag acgggcaggc
    13561 cgggggcagg cctggccatg cggggccgaa ccgggcactg cccagcgtgg gcgcgggggc
    13621 cacggcgcgc gcccccagcc cccgggccca gcaccccaag gcggccaacg ccaaaactct
    13681 ccctcctcct cttcctcaat ctcgctctcg ctcttttttt ttttcgcaaa aggaggggag
    13741 agggggtaaa aaaatgctgc actgtgcggc gaagccggtg agtgagcggc gcggggccaa
    13801 tcagcgtgcg ccgttccgaa agttgccttt tatggctcga gcggccgcgg cggcgcccta
    13861 taaaacccag cggcgcgacg cgccaccacc gccgagaccg cgtccgcccc gcgagcacag
    13921 agcctcgcct ttgccgatcc tctagagtcg agatccgccg ccaccatgat tgaacaagat
    13981 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
    14041 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
    14101 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
    14161 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
    14221 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
    14281 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
    14341 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
    14401 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
    14461 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc
    14521 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
    14581 ttcatcgact gtggccggct gggtgtggcc gaccgctatc aggacatagc gttggctacc
    14641 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
    14701 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
    14761 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
    14821 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
    14881 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccccggatc
    14941 taagctctag ataagtaatg atcataatca gccatatcac atctgtagag gttttacttg
    15001 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
    15061 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
    15121 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
    15181 tatcttatca tgtctggatc cctaacttta aataatgcca attatttaaa gttaggaatt
    15241 aattcaccca gctttcttgt acaaagtggt tgatatctct atagtcgcag taggcggtat
    15301 gcttgaatgt atgttttttc ccctgatgag ccttctagtt actatgggcg atgtgtgggc
    15361 cccatggact atttggctga ctcctagctc cacaccctct tggccctctt cattcacaat
    15421 ggccatttta atggaaatgg tcagtcctta gaaaaacctg gaatgtgtat tatgtcgatt
    15481 ttataggaat agacaagtgg gatttgttcc ttcaactcca attatcaaaa agattaaccc
    15541 tttgccctct acattctaat tgtaaccctt cagaaacaga ggttatttgt acaagatagc
    15601 tgaaaaaaaa tggggctgga tgccaagagg actgtttctt agtagaagga ggcagcaatc
    15661 attgcccaca tttttctctt gtgttgcagg cgtctgtgga ggcttctggt gaaattgcat
    15721 tatgcaagac tggatttcct gaagatgttt acagcgcagt cttaccgaag gatgtgcacg
    15781 aaggacagcc ccttctcaat ggtagtacct taaaagacat gcttgtctct acagtgtgtt
    15841 cggagttctt cagaatgctt ctgaggcatc tctgtggcag agggagtgtg agattttcac
    15901 cgtctcccta atgagacccc gggagttggt cactaaagac aagcttatgt atttcctgtg
    15961 tatattgttg gagggctcct ggcctgagat ggcgcaacgc aattaatgat aacttcgtat
    16021 agcatacatt atacgaagtt atggtctgag ctcgccatca gttcattcat gtagtctgaa
    16081 gaacgatatt tattagaaat ctgattctaa ggataaaaaa tgatttcttt gaatttcaga
    16141 aagggtctca caaataaata ttaacttgga ttaaaaatgc tcaattctaa tatttcagct
    16201 acattatagt aaacaagttc aacaagtaga gtctcagatg gaaacatgga atctgtggtt
    16261 ttgttacggc attgtttata gaaagtacta gaaagctcat ctatatagag cgtacgtgaa
    16321 tctcagatac agactcactc atttttcact taaagatgat caagttcatg atggttactg
    16381 tgtgagaact cttgccaaca agactagatc aaaatcagaa ataaaaccta ccaaaaacaa
    16441 actttaaagc cccttccttt agttccagtg ggaaagaaat caaggcgggg agagtagcag
    16501 tattgccatg agcacattta tcactgcaca cctgctattg aaataggaaa gcaagaattg
    16561 accagtgagt cccgtttaaa ttttccttcc cttcaaatct tgcccatctc taagggtgtc
    16621 tgagagaact gcatttagaa aatttgttaa cctcagctaa ttaatttgca cagagctcta
    16681 ccacatagcc ttaattgaag tgtctccatg gcagtgacaa acctggtgct ctaataccta
    16741 agaatcctgg aggcagagga ctttgaaatg taaatggccc cttagctaag gaagtttgtt
    16801 cctgcaactt acattttatt ttagtttcta tttaaattat gatttgttcc catctatgtg
    16861 gtctttctga agttgatttt taattttctc aaaagcatcc tcctgtccct gcctcattac
    16921 atgttctttg agtgatcgag tgtaaaactg tcacaacact ggatcgctct tatctagtgg
    16981 gcaatgagtg acttgatggt ggcttaattc aaatcatagt gtgtcaacat tatctcaacg
    17041 agaatcttca agccctgcat actttctatg ttctttgaat ctgtaaatta ctaaggagta
    17101 tcctagaatc tgttttgtga gaaaataatc tataagtgca caaactatat tgacatgatt
    17161 gagaaatagt tctgctaagc aaaaagaaat tttagggaat taggttgcaa tttttcattg
    17221 tattcttgtg atttttagtg agtaatgccc ttttcaatga gcctttaatt tgtttattac
    17281 aaataggcac atagtctgtc atgtgaaacc atagacttaa aattaattag ttgggcattt
    17341 tgaatagttt ttaaaatgag agctcatttt ttggttattc aataatgcta gtaacagaag
    17401 aaataagact tagctttttg ctcatgtctt tttactataa cctagacact tgtgatttct
    17461 gctgatattg caattatgaa agattcaaaa tatttttggc ttttaatgcc agccatccgt
    17521 catgagaaaa gaatatatga tagtgtgttg ttttcctcag gctataccat cactagtttc
    17581 tttatccttc ttagaagatg ttaaagaaca taccaccacc cgaaagctca aggaattctg
    17641 ttttaagatg cccactagtg aaaagagtaa ctttgggaaa gaaaggaatt cactcaaaca
    17701 tctgaggagc agtacacttg tctttccaag agtcgccaat tattttcctg tcagagatct
    17761 gaccataaat gcttgcaagg ttttatcttc tatatatttt tggagtcttt agtaggctga
    17821 taatccaaag ctgtgccatt cacggaagcc aaatgccatg ccataattaa atgcattagt
    17881 ttggatactg cagtaattca atttacttta tacacatggc attgctaagc caggaagggt
    17941 gattgcttct gctgtgtgct agtgatctgt aaggtttaaa gaaacaattg tccgtgaaag
    18001 tccatgaccc ttccccaagc cgttggcaga ctggggtgtt ccaccataat taatgaatta
    18061 ccaccattga acttcatctt attgtcattg tgctgtcttg cctgagttgc agaacattcc
    18121 tttctaaatc ctgtagctta tatgtgtcct ttaaagttag aatcagtttt gttattctag
    18181 ccagtcattt taattctgta ttttcaacat tcgataacat cattcttggt atttcctttg
    18241 gtttcttact gatgatattt tcaaagagga cagtggttca cccattgcat gctgtgatgt
    18301 cacaggacac gaatgacata taaaggttca ggaaagcctt aagagtcttg gtgtttagtg
    18361 tagggctgct gatcattttt caacagttga gtagtaatct tcacaaaggt gctaggcaag
    18421 gattaagctc tccttacaga cttgagatga cgttagagaa gccacagaag atgtggcatg
    18481 gacagtcact aaagaacaac ttgtccccat cctcccgttc attcatcaga caacacattt
    18541 caagaaccag ttaggcactc cagtgtctct tccagatccc gtaagtttgt acttttgtaa
    18601 aaccacgaag agaatgatgt gacttatgct cagccttcgc ctcccctttc atagtatttg
    18661 aatactttag ctcattaaag agagtccaca atttttttaa atagctatat agccatttta
    18721 tgtaggcttg tgttctgtgt tgctagcaaa accagatttt tcttagctgt gtgtctattt
    18781 ttaaacatac aggttgtttc caattgtctt ctctgatgca tagtgctgct gggaagatcc
    18841 cagagaaagt taatgtttct ttagggacat cgtcttccgg aacttgatca aagtagaatt
    18901 tccaggtcaa aggaaaaata gaatttggga gatggagatt aatgttcttt ctaagtgtgt
    18961 gtgtgcatgt gcgcatgtgc ctgccttgcc gtgttagagc ttcatgtgtg tgtgtgcatg
    19021 catgcatgtg cccaccttgc cgtgttagag cttcatgtgt gtgtgtgcat gcgtgcatgt
    19081 gcccgccttg cagtgttaga gcttcatgtg ttggtccttg ccttccatct tgctggagac
    19141 tgtatttcct actcctactg gctgtctcag gttagctgta ctgcgagtgt ccaaggcttc
    19201 tgcttctcct ccgccatctc cctgtactgg gattccaggt gctcactact ctgtttcatt
    19261 tctggggatc caaactccgg tcctcagtga aagtctctgc ccattgacct ctgctctcca
    19321 gccctgggaa aatgtccttt gtaggatatt ttttttttaa attaaaagta tgttacttca
    19381 ttttaaaatc ttcatcacca tgtaattttg taatgttttc tagctttgtt ttatcctttc
    19441 tggtgcaaag cagttttcaa tcctatgaga tatatgtttg ccttcatgaa ttaggatcag
    19501 atctatcgtg tttagggagt catttttact ttacattttc ctattccagt ttcaaagcac
    19561 ttattaccaa ctctctcatt ttccagacaa gcaaattaga gctctgagag attataggct
    19621 tgcccatgat tacatagctc cttagtctga tagctgcaga cctggaatta aaactcaggt
    19681 tttttcccct aacctagttc tgatgatgat gataattaaa aagaaaaaaa aaaggcccac
    19741 cacagagatg gggaaggttc tagggaacta gtccacgtat ccaggtgtct tgtgccaagc
    19801 ttataggaga ttcactttct ctcctgacat aatgatttac atgttgctgg gtagatttgg
    19861 agaccatcac ataaacaata cctgcctctt tctccttctg tctttctctt tattgtctgt
    19921 ctgtctgtct agatatatgt ctcttaataa tttttctctc agtcatttat acatgcctac
    19981 ttaataggaa aattattgaa ctctggaaga taatatttaa tttaaactcc actaaactag
    20041 aaagaaagct ttgtgtgagt gcattgcttg aaaagaaagg aattactatc ttccttatgt
    20101 ccacagtgtc tgacacagca acaacaacca gcttgacatt ggagaggatg atagggtttt
    20161 cagtgtgcca ggcaagaggg gtctccttgt gtgtcttgca tgggtttcac caagtcagct
    20221 cctccagagc tggttaacag agacagcaac tggaagttac catgactatg ttcggtagtc
    20281 catactgttg cacagcctct gggaagctcg tgatgaccgt gttcaactca ataggatttc
    20341 attcagatta gttggcttgc tgatcaggcc tgttctgcat taacatgggg ctggaggatg
    20401 ttagctgcag acaacatgac agaaatagtt ttaatttgtg tagtaggagg atagggctgg
    20461 aaca
//