LOCUS       91722#pL1L2GT1_LF2A_nEGFPOT2A_CreERT2_pAbActNeopA#L3L4_pD223_DTA_T_spec        20765 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2GT1_LF2A_nEGFPOT2A_CreERT2_pAbActNeopA
COMMENT     design_id: 91722
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000033826
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            7113..7241
                     /db_xref="ENSEMBL:ENSMUSE00000209620"
                     /rank="2"
                     /note="ENSMUSE00000209620"
     misc_feature    4275..10146
                     /note="5 arm"
     misc_feature    10174..10198
                     /note="B1 Gateway"
     misc_feature    11165..11182
                     /note="FCHK QC critical region"
     misc_feature    11149..11164
                     /note="FCHK QC prefix region"
     misc_feature    11183..11188
                     /note="FCHK QC suffix region"
     misc_feature    11311..11362
                     /note="F2A"
     misc_feature    11188..11310
                     /note="1Dleader"
     primer          11034..11055
                     /vntifkey="27"
                     /dnas_title="seq primer FCHK2"
                     /label=seq primer FCHK2
                     /note="seq primer FCHK2"
     exon            11134..11187
                     /vntifkey="61"
                     /dnas_title="mouse En2 exon"
                     /label=mouse En2 exon
                     /note="mouse En2 exon"
     misc_feature    12234..12234
                     /note=" A to G to kill RsRII site"
     misc_feature    12184..12237
                     /note="self-cleaving' 2A peptide"
     misc_feature    12238..13263
                     /note="Cre"
     misc_feature    11368..11457
                     /note="3X NLS"
     misc_feature    11458..12174
                     /note="EGFP"
     primer          complement(10327..10348)
                     /vntifkey="27"
                     /dnas_title="LRPCR LAR3_EN2"
                     /label=LRPCR LAR3_EN2
                     /note="LRPCR LAR3_EN2"
     intron          10259..11133
                     /vntifkey="21"
                     /dnas_title="mouse En2 intron"
                     /label=mouse En2 intron
                     /note="mouse En2 intron"
     primer          complement(10223..10246)
                     /vntifkey="27"
                     /dnas_title="FRT3_oligo"
                     /label=FRT3_oligo
                     /note="FRT3"
     misc_feature    10211..10258
                     /vntifkey="21"
                     /dnas_title="Frt"
                     /label=Frt
                     /note="Frt"
     misc_feature    14524..14531
                     /vntifkey="21"
                     /dnas_title="polylinker"
                     /label=polylinker
     misc_recomb     14492..14523
                     /note="Rox(inv)"
     misc_feature    14484..14491
                     /vntifkey="21"
                     /dnas_title="RE sites"
                     /label=RE sites
     primer          14424..14442
                     /vntifkey="27"
                     /dnas_title="seq primer NF"
                     /label=seq primer NF
     primer          14298..14321
                     /vntifkey="27"
                     /dnas_title="LRPCR RAF5"
                     /label=LRPCR RAF5
     misc_feature    14241..14483
                     /vntifkey="21"
                     /dnas_title="SV40 pA"
                     /label=SV40 pA
     misc_feature    13273..14217
                     /note="ERT2"
     misc_feature    15731..15731
                     /note="G to C conservative mutation to kill RsrII site"
     misc_feature    16079..16322
                     /note="SV40pA"
     misc_feature    16137..16160
                     /note="RAF5"
     misc_feature    15886..15905
                     /note="neoBstB1_5"
     misc_feature    15593..15611
                     /note="neoSphI_5"
     misc_feature    15256..15275
                     /note="neoPstI_5"
     misc_feature    15087..16072
                     /note="neomycin resistance "
     misc_feature    14937..14956
                     /note="betact5"
     promoter        14541..15061
                     /note="human beta actin promoter"
     insert          14238..16317
                     /source="pL1L2_GTIRES_BetactP FLFL MapVerified"
     misc_feature    16318..16322
                     /note="for bActNeo"
     misc_recomb     16324..16355
                     /note="Rox"
     misc_feature    16368..16388
                     /note="B2 Gateway"
     misc_feature    10147..16418
                     /note="Synthetic Cassette"
     exon            17057..17256
                     /db_xref="ENSEMBL:ENSMUSE00000209623"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 1 ENSMUSE00000209623"
     exon            16819..16932
                     /db_xref="ENSEMBL:ENSMUSE00000209619"
                     /type="targeted"
                     /rank="3"
                     /note="target exon 2 ENSMUSE00000209619"
     misc_feature    16419..17606
                     /note="Critical Region"
     misc_feature    17630..17663
                     /note="Downstream LoxP"
     misc_feature    17607..17686
                     /note="synthetic loxP region"
     exon            20086..20664
                     /db_xref="ENSEMBL:ENSMUSE00000883277"
                     /rank="7"
                     /note="ENSMUSE00000883277"
     exon            19334..19444
                     /db_xref="ENSEMBL:ENSMUSE00000492170"
                     /rank="6"
                     /note="ENSMUSE00000492170"
     exon            18054..18110
                     /db_xref="ENSEMBL:ENSMUSE00000209622"
                     /rank="5"
                     /note="ENSMUSE00000209622"
     misc_feature    17687..20765
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggactaata ttttaagtta tagaaagtga tgatagtttc ttgttcaggt
     4321 cacttggggg gcagggattt atttcagtga tcttatccta tggtgagagt tgccatgact
     4381 ctaacatccc aggtgtggtt ggagatatcc ccggtttcca ggctatatct gaaaatagcc
     4441 ctgtgtagct atcgttctta ggtatggact aggtaggcta ggcaggtata ttcagagggt
     4501 ttttttccct tatgcctgcc ctgtggcgac ctgggaggtg gtcaggtggg gggacacttc
     4561 cttctggggc tactatagga gcagaaaagt cctcactccc aggaggctgt gcacctgttc
     4621 agaatacccc ccccccaaca cacacacaca gagtaagtct tccacaaaca tgaagtactc
     4681 ctgagacccc aaagagtaaa tatctttcaa gttctccagg acccatggcg tgggagctac
     4741 agacagcgct ggggagggca gtgaaggctt tacagggcta tacactggag aatacaaaca
     4801 cagggtgggt gccgccttac tccttagaag gcaggtccca gggcctgtcc ttttgtgttc
     4861 ccacagcagc cataggggtc tatagtccaa ggtcctccag gtctgcgcac aaattaaagg
     4921 ccgttttggc tgacccacgc agacccatct cgggggctcg ggaactgaga tgaggttcac
     4981 agagagagga acctgctgat tcagctagcc ccagctcagc ctggacagac tactacatgg
     5041 aaaacacact gttcatgcag agggagatgt ggtacacccc cctcaagcct ctgagacccc
     5101 ctagggcaga gagcgcccct gagtcccagg tgacttagga aaatacaacc agttccaagg
     5161 cggagaagaa acagtccacc agtgggaaac agaagcagag ctggttctgg gaccatccca
     5221 ggaagtgggt cacctgaaga agggacagca gatagagccc tgaggcaaat gacagatggg
     5281 ggtgggttgc caactatccc ctcgggaagc ttgtacagag gccattgtag ctgccaagcc
     5341 tccttgatga ctataatgcc actcagaact ttctggaatg gattcacagc ctgatcagaa
     5401 gacatggact gggtgtctag cgatgacaat ctcaggaaga aagcatactt ccagtgaaat
     5461 gacagaatcc aagttactct taggggagga ggcacacaca gaggaaacgg ggggtggggg
     5521 ggacagagat catagactga gaacagcata cataagtctc agtgacttgg ggcacaccca
     5581 gccacctggg ccacactctc tgtgtggcat ggaactctaa ccaacagtgg ctctcgggct
     5641 gtggggttcc acggcattgc ccacgtgagg aatcagactt gatggtggca aggttgggat
     5701 ccttcgactg gagatgttga gccctggctc acagctggta agtcagtaag tgggtctatg
     5761 agggggacgg acaagcagca aagctgaggt tcctccctgt gggagcttgg gtcccgggaa
     5821 ggtagatgag ttggccagaa gccacatagg gaactgtagt gagcacgcca gccctccgat
     5881 gggaaatgcc ctatgctggt cctttcagaa caagcttgga cattcgcccc tgtagaccat
     5941 gcagctaagt aggtatacaa tgctgtcaat catatgagca caaggccagg cgattggatg
     6001 ctacaacagc caaacccaag aggagtacag cgtctccgtg gaaccacagt tctgagacaa
     6061 gccactgctg ctctggccct ccagggaggc cctgttggtc ccacagctgg cctctcgcct
     6121 gcaacctccc caccgtctcg catgccctgc tctcatgccc tctctgctta ggcctgctct
     6181 ttagtaactt ttggaggtca ctggagaatg acacacccac tctgccgtgc attcagaaga
     6241 ttttctgagc acacattttc ttaagagatc ttcccaggaa gccaaagtca agagcagcac
     6301 aaaagacttt tcctgccagc atatttcttc gcttggctag aaattatcaa aacatgacgg
     6361 tgggcacttc acagtatccc aagcccaagg ctgtgcccca gaagagtgcg gatcagactt
     6421 gaatttgaaa gaaatgtcac cgggaagtct tcagtcagga gcccatggag cctggctcac
     6481 atccgggttg gaacacttgc tgctaaccat ggctcctcta cagatgatgt ctgaagcaac
     6541 agtgttgaga gctgtaggag ccgcacccac ctggcatgcc ctatgtccct cttctctgta
     6601 ggtcatgggg caaactcctg ccaggtcctc ggcacagcct gatacatacc cagccacagt
     6661 cctacccgat cagatcgtgc actgggggcc tccttggtca gcagctgcct ttgtcttcct
     6721 gtaatatgaa cagaagagac tccagggtgt ggagggatag gctctgggat tcagccttgc
     6781 tggtgatcag tataccaagc acacagggtt gtcactaaca tgcaagggca gtgagtgaca
     6841 ggagccacgc aaggccatgc acatgccctg tagctctgga gtagaacagt gagttccagg
     6901 gccagggagt gagggtaaag ggggcaccaa agagctcaga gattctgctt gggtgagaaa
     6961 gctctggaac ttggtagggg tgctgctggt gcagcatcat gagagcattg cacggcacaa
     7021 cctgtgtgct gggctggaag tggagaaagc gtggctcagt gccatatacc ttgccatggc
     7081 tcaggtggct ccttccaact ctctcttcac agtgtggatc agcctgtact acgcaggttt
     7141 ctatgtggtc atgaccgggc tctttgcctt gtgcatctat gtgctgatgc agaccattga
     7201 cccctacacc ccagactacc aggaccagtt aaagtcacca ggtaagcatt gggagggctt
     7261 cggcaatgct cttggggcaa catacagtag accatagcag agtatctttc tccctacacc
     7321 tgggacaggg gtccccacag attcctgaga ggaacaatga ccccaaattg ccaagtctga
     7381 cttagacttt agttttatgt aataggagtg gcttcagggg gatggtcaca gagacaagaa
     7441 acctttgcgt tcatgttata tgttatatgc ttatgcacac acaggagagt ttctgggagg
     7501 gtacagaaga ggttgctggc agggagtagg cctggtgact ccagaaggat ctagggtgga
     7561 gatgccccct agctcattag tccctgggac agaagagaga agagatgaag ctgcgccgtc
     7621 tgaggctgag aaacgcgaaa acaagtcctt cccgctttga gccccgtaca gctgctctcc
     7681 tcgtaacacc catagaagca gcaaagccct gtccactccc actgtgtggt tccaggcatt
     7741 gcactcagag catcgggctt ggtggcaagc tccttttacc tgccaagccc tcttgctgca
     7801 caagggaaaa aacatatttt tacgattttg ggtttggtgg gttttttttt tctttgtttt
     7861 atgtgcatgg ctgttgtctt agtcagggtt tctattcctg cacaaacagc atgaccaaga
     7921 aacaagttgg ggaggaaagg gtttatttgg cttacacttc catgctgctg ttcatcacca
     7981 taggaagtca ggactggaac tcaaacaggg cagggagcag gagctgatgc agaggccatg
     8041 gagggatgtt ctttactagc ctgcctctcc tggcttgctc agcctgctct cttatagaac
     8101 ccaagactac cagcccagag atggcaccac ccacaagggg cctttccccc ttgatcacta
     8161 attgagaaaa tgccttacag ttggatctca tggaggcatt tcctcaactg aagctccttt
     8221 ctctgtgata actccagctg tgtcaagttg acacaaaact agccagtaca attgacccct
     8281 tgtcaacttg acacacaaac acatcactag taagcctcaa cccttacatt cttattcatc
     8341 cccaagatct aaacaacttt aaaagtccca cagtctttac atattcttaa attttcaatc
     8401 tctttaaaat atccatctct tttaaaatcc aaactctttt tacaattaaa agtctcttaa
     8461 ctgtgggctc cactaaaaca gtttcttcct tcaagaggga aaatatcagg gcacagtcac
     8521 aatcaaagca aaaatcaatc tccaaccatc caatgtctgg gatccaactc acgatcttct
     8581 gggctcctcc aagggcttgg gtcacttctc cagccatgcc ctttgtagca caggcgtcat
     8641 cctctaggct ccagatgcct gtactccact gctgctgctg ctcttggtgg tcatctcatg
     8701 gtactggcat ctccaaaaca ctgcatggtc ccttcagtcc tgggccttca attgcaactg
     8761 aggctgcacc gtcaccaatg gccttccatg gcctctcaca gtgccgagcc tcagctgctc
     8821 tgcatgaccc cttcatgcct tcaaaaccag taccacctgg gtgaccctta catattacca
     8881 agtcctgctg cagcaggagt acaaccttgg ctatctctgg aacacagcct ctttgtgctt
     8941 tcagaaaaca cttcccagaa gatgtcacct caatgatgct ggtctcttct taatcaccgc
     9001 taatttctta actccagcta accagcatca atagtcccag taatgtaaag tttttgcttt
     9061 agtagttctg gtatcttgtt aatcacagct gattcttcag ccccagctaa ccagaactac
     9121 agaatcttca caatcaaaac agcaatggcc ctgaaaagag tctttaattt tccctctgaa
     9181 atttcacaag ccaggcctcc atcttctgca ctgttctcaa cattatcttc caagctccca
     9241 cacaacatcc cacagagctc ttaacaacga atggatcttc aagaccaaag ttccaaagtc
     9301 cttccacaga cctccccaaa acatggtcag gttgtcacag gaatacccca ctctgctggt
     9361 accaatttgt cttagtcagg gtttctattc ctgcacaaac agcatgacca agaaacaagt
     9421 tggggaggaa agggtttatt tggcttacac ttccatgctg ctgttcatca ccataggaag
     9481 tcaggactgg aactcaaaca gggcagggag caggagctga tgcagaggcc atggagggat
     9541 gttctttact ggcttgcttc ccctggcttg ctcagcctgc tctcttatag aacccaagac
     9601 taccagccca gagatggcac cacccacaag gggcctttcc cccttgatca ctaattgaga
     9661 aaatgcctta cagttggatc tcatggaggc atttcctcaa ctgaagctcc tttctctgtg
     9721 ataactccag ctgtgtcaag ttgacacaaa actagccagt acagctgttt tgcctgcatg
     9781 tgcaccacct gtgtgccaag tccctgcaga ggccagaaga gggcatcaga tgccccagaa
     9841 cttgagttac agctggttgt gagttgatat gtggctgctg gggatctaat cagggtcctc
     9901 tggaagagca gccagtgatc ttaaacgtta agccatttcc ccagtcccca atgaaaaccc
     9961 ttttattttt ttcccattga gacagaatat ctttatgtag ccctggatat cccgaaactc
    10021 tatgtaaatc aggctgggcc ccaaacagag atctgcctgc ctctgcctcc caagtgctgg
    10081 gattaaaggt gcaagccaca acatgtgttt cccaagtgaa aaatgtgaca tgtgacatgt
    10141 tcagataagg cgcataacga taccacgata tcaacaagtt tgtacaaaaa agcaggctgg
    10201 cgccggaacc gaagttccta ttccgaagtt cctattctct agaaagtata ggaacttcga
    10261 accctttccc acaccaccct ccacacttgc cccaaacact gccaactatg taggaggaag
    10321 gggttgggac taacagaaga acccgttgtg gggaagctgt tgggagggtc actttatgtt
    10381 cttgcccaag gtcagttggg tggcctgctt ctgatgaggt ggtcccaagg tctggggtag
    10441 aaggtgagag ggacaggcca ccaaggtcag cccccccccc ctatcccata ggagccaggt
    10501 ccctctcctg gacaggaaga ctgaagggga gatgccagag actcagtgaa gcctggggta
    10561 ccctattgga gtccttcaag gaaacaaact tggcctcacc aggcctcagc cttggctcct
    10621 cctgggaact ctactgccct tgggatcccc ttgtagttgt gggttacata ggaaggggga
    10681 cgggattccc cttgactggc tagcctactc ttttcttcag tcttctccat ctcctctcac
    10741 ctgtctctcg accctttccc taggatagac ttggaaaaag ataaggggag aaaacaaatg
    10801 caaacgaggc cagaaagatt ttggctgggc attccttccg ctagctttta ttgggatccc
    10861 ctagtttgtg ataggccttt tagctacatc tgccaatcca tctcattttc acacacacac
    10921 acaccacttt ccttctggtc agtgggcaca tgtccagcct caagtttata tcaccacccc
    10981 caatgcccaa cacttgtatg gccttgggcg ggtcatcccc ccccccaccc ccagtatctg
    11041 caacctcaag ctagcttggg tgcgttggtt gtggataagt agctagactc cagcaaccag
    11101 taacctctgc cctttctcct ccatgacaac caggctccca ggtcccgaaa accaaagaag
    11161 aagaacgcag atcgcagatc gcagatctct agagtcaccg agttgcttta ccggatgaag
    11221 agggccgaaa catactgtcc aaggcccttg ctggcaatcc acccaactga agccagacac
    11281 aaacagaaaa ttgtggcacc ggtgaaacag actttgaatt ttgaccttct caagttggcg
    11341 ggagacgtcg agtccaaccc cggaccggtg catgtggacc ccaagaaaaa gcggaaggtg
    11401 gaccctaaaa agaaacgcaa agtggatcct aagaaaaaaa gaaaggtgca cgtgtccatg
    11461 gtgtccaagg gcgaggaact gttcaccggc gtggtgccca tcctggtgga actggatggc
    11521 gacgtgaacg gccacaagtt cagcgtgtcc ggcgagggcg aaggcgacgc cacatacgga
    11581 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccttg gcctaccctc
    11641 gtgaccacac tgacctacgg cgtgcagtgc ttcagcagat accccgacca tatgaagcag
    11701 cacgacttct tcaagagcgc catgcccgag ggctacgtgc aggaaagaac catcttcttt
    11761 aaggacgacg gcaactacaa gaccagggcc gaagtgaagt tcgagggcga caccctcgtg
    11821 aacagaatcg agctgaaggg catcgacttc aaagaggacg gcaacatcct gggccacaag
    11881 ctggagtaca actacaacag ccacaacgtg tacatcatgg ccgacaagca gaaaaacggc
    11941 atcaaagtga acttcaagat ccggcacaac atcgaggacg gctccgtgca gctggccgac
    12001 cactaccagc agaacacccc tatcggcgac ggccctgtgc tgctgcctga caaccactac
    12061 ctgagcaccc agagcgccct gagcaaggac cccaacgaga agagggacca catggtgctg
    12121 ctggaattcg tgaccgccgc tggcatcacc ctgggcatgg acgagctgta caagcctgca
    12181 ggcgagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cgggccgtcc
    12241 aatttactga ccgtacacca aaatttgcct gcattaccgg tcgatgcaac gagtgatgag
    12301 gttcgcaaga acctgatgga catgttcagg gatcgccagg cgttttctga gcatacctgg
    12361 aaaatgcttc tgtccgtttg ccggtcgtgg gcggcatggt gcaagttgaa taaccggaaa
    12421 tggtttcccg cagaacctga agatgttcgc gattatcttc tatatcttca ggcgcgcggt
    12481 ctggcagtaa aaactatcca gcaacatttg ggccagctaa acatgcttca tcgtcggtct
    12541 gggctgccac gaccaagtga cagcaatgct gtttcactgg ttatgcggcg gatccgaaaa
    12601 gaaaacgttg atgccggtga acgtgcaaaa caggctctag cgttcgaacg cactgatttc
    12661 gaccaggttc gttcactcat ggaaaatagc gaccgctgcc aggatataag gaatctggca
    12721 tttctgggga ttgcttataa caccctgtta cgtatagccg aaattgccag gatcagggtt
    12781 aaagatatct cacgtactga cggtgggaga atgttaatcc atattggcag aacgaaaacg
    12841 ctggttagca ccgcaggtgt agagaaggca cttagcctgg gggtaactaa actggtcgag
    12901 cgatggattt ccgtctctgg tgtagctgat gatccgaata actacctgtt ttgccgggtc
    12961 agaaaaaatg gtgttgccgc gccatctgcc accagccagc tatcaactcg cgccctggaa
    13021 gggatttttg aagcaactca tcgattgatt tacggcgcta aggatgactc tggtcagaga
    13081 tacctggcct ggtctggaca cagtgcccgt gtcggagccg cgcgagatat ggcccgcgct
    13141 ggagtttcaa taccggagat catgcaagct ggtggctgga ccaatgtaaa tattgtcatg
    13201 aactatatcc gtaacctgga tagtgaaaca ggggcaatgg tgcgcctgct ggaagatggc
    13261 gatctcgagc catctgctgg agacatgaga gctgccaacc tttggccaag cccgctcatg
    13321 atcaaacgct ctaagaagaa cagcctggcc ttgtccctga cggccgacca gatggtcagt
    13381 gccttgttgg atgctgagcc ccccatactc tattccgagt atgatcctac cagacccttc
    13441 agtgaagctt cgatgatggg cttactgacc aacctggcag acagggagct ggttcacatg
    13501 atcaactggg cgaagagggt gccaggcttt gtggatttga ccctccatga tcaggtccac
    13561 cttctagaat gtgcctggct agagatcctg atgattggtc tcgtctggcg ctccatggag
    13621 cacccagtga agctactgtt tgctcctaac ttgctcttgg acaggaacca gggaaaatgt
    13681 gtagagggca tggtggagat cttcgacatg ctgctggcta catcatctcg gttccgcatg
    13741 atgaatctgc agggagagga gtttgtgtgc ctcaaatcta ttattttgct taattctgga
    13801 gtgtacacat ttctgtccag caccctgaag tctctggaag agaaggacca tatccaccga
    13861 gtcctggaca agatcacaga cactttgatc cacctgatgg ccaaggcagg cctgaccctg
    13921 cagcagcagc accagcggct ggcccagctc ctcctcatcc tctcccacat caggcacatg
    13981 agtaacaaag gcatggagca tctgtacagc atgaagtgca agaacgtggt gcccctctat
    14041 gacctgctgc tggaggcggc ggacgcccac cgcctacatg cgcccactag ccgtggaggg
    14101 gcatccgtgg aggagacgga ccaaagccac ttggccactg cgggctctac ttcatcgcat
    14161 tccttgcaaa agtattacat cacgggggag gcagagggtt tccctgccac agcttgatga
    14221 agatctgagc tcttaattaa tgatcataat cagccatatc acatctgtag aggttttact
    14281 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
    14341 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
    14401 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
    14461 tgtatcttat catgtctgga tccgtttaaa ctaactttaa ataattggca ttatttaaag
    14521 ttacatatgg cggccaattc gccttctgca ggagcgtaca gaacccaggg ccctggcacc
    14581 cgtgcagacc ctggcccacc ccacctgggc gctcagtgcc caagagatgt ccacacctag
    14641 gatgtcccgc ggtgggtggg gggcccgaga gacgggcagg ccgggggcag gcctggccat
    14701 gcggggccga accgggcact gcccagcgtg ggcgcggggg ccacggcgcg cgcccccagc
    14761 ccccgggccc agcaccccaa ggcggccaac gccaaaactc tccctcctcc tcttcctcaa
    14821 tctcgctctc gctctttttt tttttcgcaa aaggagggga gagggggtaa aaaaatgctg
    14881 cactgtgcgg cgaagccggt gagtgagcgg cgcggggcca atcagcgtgc gccgttccga
    14941 aagttgcctt ttatggctcg agcggccgcg gcggcgccct ataaaaccca gcggcgcgac
    15001 gcgccaccac cgccgagacc gcgtccgccc cgcgagcaca gagcctcgcc tttgccgatc
    15061 ctctagagtc gagatccgcc gccaccatga ttgaacaaga tggattgcac gcaggttctc
    15121 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
    15181 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
    15241 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca
    15301 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
    15361 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
    15421 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
    15481 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
    15541 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
    15601 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
    15661 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
    15721 tgggtgtggc cgaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
    15781 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
    15841 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga
    15901 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt
    15961 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
    16021 cggggatctc atgctggagt tcttcgccca ccccccggat ctaagctcta gataagtaat
    16081 gatcataatc agccatatca catctgtaga ggttttactt gctttaaaaa acctcccaca
    16141 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
    16201 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
    16261 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
    16321 ccctaacttt aaataatgcc aattatttaa agttaggaat taattcaccc agctttcttg
    16381 tacaaagtgg ttgatatctc tatagtcgca gtaggcgggc tagaaaccct ccccagaggt
    16441 cagcatctac tgcaccaatc ctgctcatga ccctcaccgc cccccaggct cagagtcccc
    16501 caaacaccaa gtgtcactat tgctgggctg ggcatccaga gtctcaggca caggacaggc
    16561 cttgacgccc actgtgctgc attctgcaga ggtcaggggc cccaggccag tgtcctcaga
    16621 ggctgtggcc ttctttagag cattatcgcc aagcttgttc agctacaggg agtctgctct
    16681 gcggtcccca cctaggaggg cgtcaggggc agctgcctat ggtcggtgct tacgggagtc
    16741 tcaacaacct cagagccaag ggctttgtgt aagtgagtgt taggagaaac tgatcaagga
    16801 ggcttgcttt ccttgcaggg gtaaccttga gaccggacgt gtatggggaa agagggctga
    16861 agatttccta caatgtctct gaaaacagtt cctgggctgg cctcacacac accctgcaca
    16921 gcttcttagc aggtgagtga ccacaaatgt agcagcgtgt ctgcttccta cgagaatggc
    16981 tggggatgca ggacctcctt catggaccgt gggggtcagg gaggggctgg ctgctgagag
    17041 gatgtgtgtt ctacaggcta taccccagca tcccagcagg acagcatcaa ctgtacatct
    17101 gaaaagtact tcttccagga gagctttgca gctccaaacc acaccaagtt ctcctgcaag
    17161 ttcactgcag acatgctaca aaactgctca ggcctggcgg accccagctt cggcttcgag
    17221 gaggggaagc cctgcttcat tattaaaatg aacagagtga gttacaaggg tcctgctctg
    17281 gaaatggccc ctccccacac tcaacgatgg gggactgggc ccttcatcta cccacccgga
    17341 ctccacacac actcatcatg ttctgctctt aagaaatctt agccagcagg ggtcttgagt
    17401 tgttgattca gactggagaa gcagggggta ttctacatgg gctccttctg catcagagac
    17461 aggaatctgt cagggtaaat cctataggaa ccactgaggt tctggtggta tcagaagcca
    17521 agggacagca agtcccgtca atgccctaag aacaccgaca cagtaagcaa gaggcagaaa
    17581 tgcagtcaaa ctgagtgaca ctcagagaga tggcgcaacg caattaatga taacttcgta
    17641 tagcatacat tatacgaagt tatggtctga gctcgccatc agttcagtct ccccagagat
    17701 gcctggcaag caggtggtga tcagagctcc acgggtccat gccccagttg ccactcctgc
    17761 caaaggcagg tcctgcacag agctcaggag acaatgactg aggctcaaga agtctaggaa
    17821 gggatgtgag aatgtgggga ccccactgag agcagtaggt agacctgttc tgcagcaagg
    17881 aaggctgcta atcaccacag caggcatggg tgtggtgcca gtgactagga ccctctatct
    17941 cagctctacc actgccagct gccctcctac actgtggtac catgggtaat ggctcagaat
    18001 tgtgtgtgaa tgtctgcctg cacctcaagg gcacatggca ccctttctcc tagattgtca
    18061 agttcctccc cagcaacaac acagctcccc gagtggactg caccttccag gtgagcacag
    18121 ctggggtggg gcagggcgga gcggggccag gatgggtgga gcaggagatg ggataggacc
    18181 caggttaacc ctggaaactt gcgaacctca tctgcccatc cgctgctgac aggagtgatg
    18241 acacagaggg aaaatgctat agccatggtt ttcccctcat ccggtctgtg cagctactca
    18301 cgcttcagaa agcccggcat tgctgtgggt ggcctacctt ctgcactgtc tatattttgt
    18361 atttacagaa atattccaga tcgtcaacag catagttgtt taatggggtt tggcatgtct
    18421 acttaggtgg tatggatctc agagaaacct cttgcaaatg tctaagtaca ttatcaaatg
    18481 ctaaacttag ccttcactaa atgtttccaa atgtttcaat gctgagccct aggtgataca
    18541 tcaaaagggg aagttgataa gtggtccagg gaacatctgt cccctagagc tctgtcccta
    18601 gggcttcagg catggtcttc actttttttt taaagattta tttattgtta tatgtaagta
    18661 cactgtagct gtctttagac acaccaggag agggtgtcag atctcattac agatggttgt
    18721 gagccaccat gtggttgctg ggatttgaac tcagggcctc tggaagagca gtcagtgctc
    18781 ttaaccgctg agccatcagc cctggtcttc actttattat tatttttttt cccacagaca
    18841 agctaacctg agatttgaga ggtcattact ccttgttgag tgtaagctac tcattgggca
    18901 ggtctgacgc caacaaaaaa aggaactaga tcgtaggaac acgtgcttgc acttaatcca
    18961 gaaacctgcc aagactctga acctgctttg gcagacgagg tcttggactg gtcagagctt
    19021 ccctctcaga agctagcaac cacacaccgg cttccccagc tccatcgctc agccaggact
    19081 ctacgatggg actctctttc tctaggtgga aagtgcctga ggcttgcatt ttgaggaatt
    19141 atggtcttgc aggctctcag ccctgactat ggaagctgac tagttgcaag gctaactcta
    19201 ttacactagc atgcaaacac atgtcgtgtg atcactggca tcggtaccag tgcgtgccac
    19261 acatgtggac ttgtacgcaa ttgaacatgt gccccaatgc ctatcagact cagctgtggc
    19321 ctctcctccc caggatgatc cccaaaagcc ccggaaggac actgaacccc tgcaggtcga
    19381 gtactatccc cccaatggca ccttcagtct ccactatttc ccctactatg gcaagaaagc
    19441 acaggtaggt ctgcttcact ctgccacagg gcactcgctg aattacctca gacttagcaa
    19501 cagggaagcc agtttctatt aaaaaagaaa agaaaagaaa agaaaaaaag cagagcaaag
    19561 taatcccatc agcacttggg aggcggaggc aggaggatcg tgagcctaag gtcattatgg
    19621 ctacatagca agttcaaggc caacctgggc tgcatgagat tctcttttgg aaaaaatgaa
    19681 caaaagaaag gcatgattta gccagtcctg aacacttctc atatccacct aaaaatgcac
    19741 ccccacaccc ccaaaaagtc atggctatca gcctgattcc cttagggtgc aggctctccc
    19801 acatcgtcta gtgtctgaga atctctccaa agtgatttct ctctccgtgc tgctctcacg
    19861 tgctgtttgc catcggcagt cccaagagtc actgctccta gtcgctctgt gggcagacac
    19921 cagccgctct gtgatccaag ggctcctttg ggccagacag acctgacccc aggacagacc
    19981 cacagaggag tcctttatgc ctgcccctcc cctcttcagt tagccttgtt tttcttgaca
    20041 tcagggtttt gtcaagcatg tcacccccct gtgcttccac ttcagcccca ctacagcaac
    20101 cctctggtgg cagccaagct cctcaacgtc cccaagaaca tgcaagtcag catcgtgtgc
    20161 aagatcctgg cagaccacgt gaccttcaac aacccccatg acccctacga agggaaggtg
    20221 gagtttaaac ttacaataca gaagtaaaac gtgggagtgg tgtccacccc tcagatgggt
    20281 ggacacggat ggacactctt cctgactgca cagcctctgc tggccagcat gcacggccac
    20341 ttccatcatg gcccagagct gacaccagga agtgctccag cccagcactc cccgagtatc
    20401 accaaccctc acgtgagcgc aatcctatcg gagctgtggg gttaacacgg ggacccagag
    20461 tccggctgcc actaaggaag gcctatctgt ccacatttct gtcctgtgat catttgcccg
    20521 tcctgcactt caatatgaac tatgggtcca catcagggta acactggtca ccctggcatg
    20581 acacatctcc agttcgtgct tctgagacca cagtccctat gccatcaaaa caaactatat
    20641 taaagttatt cttattctgt gtgaaaaatc catgcctttc ctgaccccag gggaaatttc
    20701 tactaagaac tgaacacccc tttatctaaa acctttctgt gatttcaacc gcaatacaag
    20761 tgtct
//