LOCUS       87049#pL1L2GT0_LF2A_nEGFPOT2A_CreERT2_pAbActNeopA#L3L4_pD223_DTA_T_spec        22205 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2GT0_LF2A_nEGFPOT2A_CreERT2_pAbActNeopA
COMMENT     design_id: 87049
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000029871
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     misc_feature    4275..9902
                     /note="5 arm"
     misc_feature    9930..9954
                     /note="B1 Gateway"
     misc_feature    10921..10924
                     /note="FCHK QC critical region"
     misc_feature    10905..10920
                     /note="FCHK QC prefix region"
     misc_feature    10925..10930
                     /note="FCHK QC suffix region"
     misc_feature    11053..11104
                     /note="F2A"
     misc_feature    10930..11052
                     /note="1Dleader"
     primer          10790..10811
                     /vntifkey="27"
                     /dnas_title="seq primer FCHK2"
                     /label=seq primer FCHK2
                     /note="seq primer FCHK2"
     exon            10890..10929
                     /vntifkey="61"
                     /dnas_title="mouse En2 exon"
                     /label=mouse En2 exon
                     /note="mouse En2 exon"
     misc_feature    11976..11976
                     /note=" A to G to kill RsRII site"
     misc_feature    11926..11979
                     /note="self-cleaving' 2A peptide"
     misc_feature    11980..13005
                     /note="Cre"
     misc_feature    11110..11199
                     /note="3X NLS"
     misc_feature    11200..11916
                     /note="EGFP"
     primer          complement(10083..10104)
                     /vntifkey="27"
                     /dnas_title="LRPCR LAR3_EN2"
                     /label=LRPCR LAR3_EN2
                     /note="LRPCR LAR3_EN2"
     intron          10015..10889
                     /vntifkey="21"
                     /dnas_title="mouse En2 intron"
                     /label=mouse En2 intron
                     /note="mouse En2 intron"
     primer          complement(9979..10002)
                     /vntifkey="27"
                     /dnas_title="FRT3_oligo"
                     /label=FRT3_oligo
                     /note="FRT3"
     misc_feature    9967..10014
                     /vntifkey="21"
                     /dnas_title="Frt"
                     /label=Frt
                     /note="Frt"
     misc_feature    14266..14273
                     /vntifkey="21"
                     /dnas_title="polylinker"
                     /label=polylinker
     misc_recomb     14234..14265
                     /note="Rox(inv)"
     misc_feature    14226..14233
                     /vntifkey="21"
                     /dnas_title="RE sites"
                     /label=RE sites
     primer          14166..14184
                     /vntifkey="27"
                     /dnas_title="seq primer NF"
                     /label=seq primer NF
     primer          14040..14063
                     /vntifkey="27"
                     /dnas_title="LRPCR RAF5"
                     /label=LRPCR RAF5
     misc_feature    13983..14225
                     /vntifkey="21"
                     /dnas_title="SV40 pA"
                     /label=SV40 pA
     misc_feature    13015..13959
                     /note="ERT2"
     misc_feature    15061..15643
                     /note="synthetic fragment mutates RsRII"
     misc_feature    15473..15473
                     /note="G to C conservative mutation to kill RsrII site"
     misc_feature    15821..16064
                     /note="SV40pA"
     misc_feature    15879..15902
                     /note="RAF5"
     misc_feature    15628..15647
                     /note="neoBstB1_5"
     misc_feature    15335..15353
                     /note="neoSphI_5"
     misc_feature    14998..15017
                     /note="neoPstI_5"
     misc_feature    14829..15814
                     /note="neomycin resistance "
     misc_feature    14679..14698
                     /note="betact5"
     promoter        14283..14803
                     /note="human beta actin promoter"
     insert          13980..16059
                     /source="pL1L2_GTIRES_BetactP FLFL MapVerified"
     misc_feature    16060..16064
                     /note="for bActNeo"
     misc_recomb     16066..16097
                     /note="Rox"
     misc_feature    16110..16130
                     /note="B2 Gateway"
     misc_feature    9903..16160
                     /note="Synthetic Cassette"
     exon            16769..16903
                     /db_xref="ENSEMBL:ENSMUSE00000242959"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 1 ENSMUSE00000242959"
     exon            17828..17966
                     /db_xref="ENSEMBL:ENSMUSE00000242951"
                     /type="targeted"
                     /rank="5"
                     /note="target exon 2 ENSMUSE00000242951"
     misc_feature    16161..18129
                     /note="Critical Region"
     misc_feature    18153..18186
                     /note="Downstream LoxP"
     misc_feature    18130..18209
                     /note="synthetic loxP region"
     exon            19653..19811
                     /db_xref="ENSEMBL:ENSMUSE00000242943"
                     /rank="6"
                     /note="ENSMUSE00000242943"
     exon            19907..20106
                     /db_xref="ENSEMBL:ENSMUSE00000242935"
                     /rank="7"
                     /note="ENSMUSE00000242935"
     exon            21197..21328
                     /db_xref="ENSEMBL:ENSMUSE00000242924"
                     /rank="8"
                     /note="ENSMUSE00000242924"
     exon            21693..21843
                     /db_xref="ENSEMBL:ENSMUSE00000242913"
                     /rank="9"
                     /note="ENSMUSE00000242913"
     misc_feature    18210..22205
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggattttgc tccaacaaca tgccttagag gacacttgaa atatccaagc
     4321 tgaaacctgg gacaggagac ctccttgctc ccagagccca tgtttgacaa gcttatacat
     4381 cacaggacaa gtcaccattg tacagtacaa atgggcttga cttaagtttc tggtcagaaa
     4441 gatctgtcct ttgactgaaa gacagacagc tagggagatt gcagggtcct ggctaggggg
     4501 ctgcagtaca agtctttctc cttttctgcc ctgccaggga gctcatgtca aagagtctcc
     4561 ctgatatttt ttaaaaatct atttccttag aacattatac tttaaaatct gatatgaagt
     4621 tcacacacta ggcatcacat ctatttattt aatttattta tttagcataa acagaatgcc
     4681 tactgtgagg tacaaccaat ttaaacttat ccacgactgg cttacaagta aatgagactc
     4741 agattatggt tattttattt ggctttgaca attactgggg ggtcacccct aatcgactct
     4801 tctaactgct gccctggcta cctccccagc cgtgtacccc agatacttgc tgtttctcct
     4861 ggccatgtgc tttccattca tctcccctca tgggtgcttc tactcttttc ttcctccttc
     4921 tccactggtc tctcctccaa ccaggtcaca gataccccaa gaacctctaa tcccttcagt
     4981 aacggagctg gtgaccatca gaagtctgtc tgctgctcag ctcagaatag agcagatcac
     5041 cgcttactct gtgctcccca gtagctccac tggctttcag taagacatgg actcccttcc
     5101 cctcacctcc acaccatacc atttctgtct ctcattagaa aacaaacagg cttctaggga
     5161 ataataataa aataagataa aacgaaaact aacacaccag aattggacaa aataaccaca
     5221 aggaaaagag cccaagagag acataagaaa cagagaccca ctcatgggca cgttcaggaa
     5281 gcccatggga acactaggct ggaaaactgg aagccatcat atatgtgcaa aggatctgtg
     5341 gggtagaaag aagaaaaatt ataaaaaata caataaatct tctttgaagg tctgatagaa
     5401 ctctgcagta aacccgtctg gtcctgggct ttttttgctt gggagactat tattggctgc
     5461 ttctatttct ttaggggata taggactgtt tagatcatta acctgatctt gatttaactt
     5521 tggtacctgg tacctgtcta gaaacttgtc catttcatcc aggttctcca gttttgttga
     5581 gtatagcctt ttgtagaagg atctgatggt gttttggatt tcttcaggat ctgttgttat
     5641 gtctcccttt tcatttctga ttttgttaat taggatgctg tccctgtgcc ctctagtgag
     5701 tctggctaag ggagtattct gttggtatag cttcaagttt gtctgtggtg ttattccaag
     5761 ctttctttcc ttattggttc ttgatttgct ctataattat tggaagtgat gtcttgaaat
     5821 tgtcagtaat gacagtttca tgatcatatt tctgtccact ctcccagtct ttgatgcact
     5881 ttagaggctg tctctaacat ttaggtgtgc agatgtttgc agtagctata gtttcctggc
     5941 atgctaaccc tgctatatct tgtctttcct tttgaatggg atctcattct gtagctcaag
     6001 ctattaactc attctacagc ccaggctagc aagaattttt ccccttagtt tcccaagagc
     6061 agagattaca gggaggagcc accggcctaa ctcttacttt ttatctataa caagtttgcc
     6121 tgaagctctg ttttgtctgg ttctagttta acttcaccct cactttgggc aactgtttta
     6181 ttggattaga agtcttcctc atctcaccaa ctgtagcact tgggatgtgt tatttcgtag
     6241 cccttggcct tactgagaaa cctcttcatt ttattgaaaa gctctgtgtg tcctgattca
     6301 ctagagtcct tcctgctatt tttcaggaag ctccgtcttt cagatgtttg attgtgctgc
     6361 atttgggatc atgtgtctgt gccttctgca gttcactgag cttcatggat gggtctgcga
     6421 gtgtcttctt cacatttggg gagttttagg cattctgtct tcaaatcatc ttttctcttc
     6481 attcttcctc ccctatggga attttacagt acaggtatgc atttgcttga tggtgccttt
     6541 tcaggccttc atttttgttg tcggtagttc atttttcttt ttttttccat tttattaggt
     6601 atttagctct tttacatttc caatgctatg ccaaaagtcc cccataccca cccaccccca
     6661 ctcccctacc cacccacttc ccctttttgg ccctggcgtt cccctgtact ggggcatata
     6721 aagtttgcaa gtccaatggg cctctctttc cagtgatggc cgactaggcc atcttttgat
     6781 acatatgcag ctagagtcaa gagctccggg gtactggtta gttcataatg ttgttccacc
     6841 tatagggttg cagatccctt cagctccttg ggtactttct ctagctcctc cattgggagc
     6901 ccggtagttc atttttcaag ttctctggtt ttccaccagc tcaaatattt tttcaagttc
     6961 tctcaaatgt ttattttaac taatattctt tcaactccag aaatcctact taaaaaaaaa
     7021 aaaacaacaa aatttttatg tctgtattct ctatttggca tgacttattt ttatgctttc
     7081 cctagtcttc tagagaaggc ttcctttaat cctggatcta tttaaaatag atcatttagg
     7141 atctttagca agttcaacat cagggctttc ttaggaacag tttttgtatc tacaatgtac
     7201 gttagttact tatttagctg tatgtcacac agacatttat tgaagagtgg acattttaaa
     7261 tgatgtgata tggtaatcta ggcatcctgt tgcttccttc cttagagcat attgcttcta
     7321 cttcttgcct agtggctttt atggactaag tctgaaaagt cagtattcat tgttatgttc
     7381 agccactgaa gtgcctgatc accttggtgc tcagttaatg atgggacaga gattatgtta
     7441 ggtgcattgg atcagaaagt ctcagccttc accaacattg gagcatgttg gagcacattg
     7501 gagcatgcca ctagcagtgt ggtggagagt ttacagattt ggctttccct tcatttcata
     7561 cgtgtgttga gcctcaacat cagctggggt gaggggttag agccttctct gacccttcct
     7621 gcacatgctg gcagctcggt taaatggatg atgctccaag gactaacaca cgaggtttgc
     7681 cgagtcccct gtgggcagct tattccccca gcctttcctt taaatgtgtg accagtttct
     7741 catttgcttc agctatcatt cctgccttaa gctcatcagt atccattgct gacaggtctg
     7801 aagattactt attttaaaaa catttttatt tattcttgga aaatgttaca tgtatcatgt
     7861 atgcaatgta tcttaatcat atccagctcc cactccccat tccagtttcc ccatcccaga
     7921 cccttcccaa tgcatctccc aaacaatttc atgtcttctt tttaaatgtt tatttttaac
     7981 ccctgactcc aagtagagct gcatatatgt gtgcatagta taaggtcatc cattgggcat
     8041 ggtgttccag tggccacacc tctcctaagt acagtgactc tccctccctg cagcaaccat
     8101 caactgcaat acttccaaag tgagggctgg gccatcgtag ccctgcccct acccatggtg
     8161 gaattttaac ttagttcatg cgtacaacat ccatgtcttg ttcaaaagac agcatgcacg
     8221 cgcgcgcgcg cgcgcacaca cacacacaca cagacacaca cacaccacac acacacacac
     8281 acacacacac acacacacac acacacacac acacacacac atttagtctt tcttcagtga
     8341 tgtaccctgt tccctaagcc tttgggagat attttattca caacagggac ttgctatata
     8401 gaacttgggt tggcccggtg ctatatccta agctgacctc aaacttacag caatcctcct
     8461 gcctcaaact cccaggtgct gagatctatg tgtgaagtac tatgcccacc agttttgcat
     8521 gggtcttttc agtagaagtc cgtggacatt aatagtgata agaactgact cagattaaat
     8581 agacaccagc aggttacaca atgacggttc tctggcaatg gctctgggag ttactgtctt
     8641 ctctgcccac tgccacactg tgctggtgag tctgtcctgt cccttctatc ctctttcaca
     8701 ctgagctgga aggtcagtgg ggtaaggcaa attaaaacag cacaggttgg tgcttattga
     8761 catccaggag ggttttggtc agttctgatg ctctgacaaa gttgataaat tggtgtttgc
     8821 cactatctac caacattttc atgctttact ggaggaatag cagggtctca ctccatctct
     8881 cccattgacg tcatactcgt cttaaaacac taacagtggc actgtttatt aatagccgta
     8941 atatcgacaa gaggatttgt aaagccctgt ctagccatgc tgttctcacc ttgtttgtac
     9001 acactgctga catgcgcact tctgcttcag aggcaaccat ggcctggctt tacttgtgtg
     9061 tacttttgtt actttattgt attgtgtctt ttgcccttta taatgaagct taaaggatca
     9121 atattgatca tgttctctgc tttaagaatt catgaattca tagatatctg agtttttaac
     9181 tttgctaaca taacaaacat ttcctcttgc actctaagaa tgtaattcat gcttaaaaat
     9241 tccaaatttt tatattattg tttactatta aagatggtac tttatacctc aagccatttc
     9301 cagatgggtc atataaatta tatcctatca acaaaatatc tatttctttg cagcctaata
     9361 tcaatgaaat gtacttaaaa ttattagtga agtttaacac cttttatata ttcttagttt
     9421 catgcagaat tcctaatttt tcactgttgt cttataagga gctgcatcta cctcgtgaag
     9481 agttgttgat tagtttttct atgttgtaga aagtcaccat gaagattatc attcaggaat
     9541 atgtgatgtt gaaggcactt gtagactatt ttctttaaca ttaacttgtg tcttcatctt
     9601 tgtcacaagt attaaagtac tgagtgacat cttttggttt tggcaataaa gctagaaatc
     9661 ttaatgtttc ttagatgctg gtaacatcat ggctctcaca tattcactga aaactatctt
     9721 ttttaatata attttttctt aggtattttc ttattttaca tttcaaatgc tatccccaaa
     9781 gtcccccata ccccccattc ccctacccac ccatcccact ttttggccct ggtgttcccc
     9841 tgaactgagg catataaagt ttgcaagacc aaggggcctc tcttcattgg atggctgact
     9901 agaaggcgca taacgatacc acgatatcaa caagtttgta caaaaaagca ggctggcgcc
     9961 ggaaccgaag ttcctattcc gaagttccta ttctctagaa agtataggaa cttcgaaccc
    10021 tttcccacac caccctccac acttgcccca aacactgcca actatgtagg aggaaggggt
    10081 tgggactaac agaagaaccc gttgtgggga agctgttggg agggtcactt tatgttcttg
    10141 cccaaggtca gttgggtggc ctgcttctga tgaggtggtc ccaaggtctg gggtagaagg
    10201 tgagagggac aggccaccaa ggtcagcccc ccccccctat cccataggag ccaggtccct
    10261 ctcctggaca ggaagactga aggggagatg ccagagactc agtgaagcct ggggtaccct
    10321 attggagtcc ttcaaggaaa caaacttggc ctcaccaggc ctcagccttg gctcctcctg
    10381 ggaactctac tgcccttggg atccccttgt agttgtgggt tacataggaa gggggacggg
    10441 attccccttg actggctagc ctactctttt cttcagtctt ctccatctcc tctcacctgt
    10501 ctctcgaccc tttccctagg atagacttgg aaaaagataa ggggagaaaa caaatgcaaa
    10561 cgaggccaga aagattttgg ctgggcattc cttccgctag cttttattgg gatcccctag
    10621 tttgtgatag gccttttagc tacatctgcc aatccatctc attttcacac acacacacac
    10681 cactttcctt ctggtcagtg ggcacatgtc cagcctcaag tttatatcac cacccccaat
    10741 gcccaacact tgtatggcct tgggcgggtc atcccccccc ccacccccag tatctgcaac
    10801 ctcaagctag cttgggtgcg ttggttgtgg ataagtagct agactccagc aaccagtaac
    10861 ctctgccctt tctcctccat gacaaccagg ctcccaggtc ccgaaaacca aagaagaaga
    10921 acgcagatct ctagagtcac cgagttgctt taccggatga agagggccga aacatactgt
    10981 ccaaggccct tgctggcaat ccacccaact gaagccagac acaaacagaa aattgtggca
    11041 ccggtgaaac agactttgaa ttttgacctt ctcaagttgg cgggagacgt cgagtccaac
    11101 cccggaccgg tgcatgtgga ccccaagaaa aagcggaagg tggaccctaa aaagaaacgc
    11161 aaagtggatc ctaagaaaaa aagaaaggtg cacgtgtcca tggtgtccaa gggcgaggaa
    11221 ctgttcaccg gcgtggtgcc catcctggtg gaactggatg gcgacgtgaa cggccacaag
    11281 ttcagcgtgt ccggcgaggg cgaaggcgac gccacatacg gaaagctgac cctgaagttc
    11341 atctgcacca ccggcaagct gcccgtgcct tggcctaccc tcgtgaccac actgacctac
    11401 ggcgtgcagt gcttcagcag ataccccgac catatgaagc agcacgactt cttcaagagc
    11461 gccatgcccg agggctacgt gcaggaaaga accatcttct ttaaggacga cggcaactac
    11521 aagaccaggg ccgaagtgaa gttcgagggc gacaccctcg tgaacagaat cgagctgaag
    11581 ggcatcgact tcaaagagga cggcaacatc ctgggccaca agctggagta caactacaac
    11641 agccacaacg tgtacatcat ggccgacaag cagaaaaacg gcatcaaagt gaacttcaag
    11701 atccggcaca acatcgagga cggctccgtg cagctggccg accactacca gcagaacacc
    11761 cctatcggcg acggccctgt gctgctgcct gacaaccact acctgagcac ccagagcgcc
    11821 ctgagcaagg accccaacga gaagagggac cacatggtgc tgctggaatt cgtgaccgcc
    11881 gctggcatca ccctgggcat ggacgagctg tacaagcctg caggcgaggg cagaggaagt
    11941 cttctaacat gcggtgacgt ggaggagaat cccgggccgt ccaatttact gaccgtacac
    12001 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
    12061 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
    12121 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
    12181 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaactatc
    12241 cagcaacatt tgggccagct aaacatgctt catcgtcggt ctgggctgcc acgaccaagt
    12301 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
    12361 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
    12421 atggaaaata gcgaccgctg ccaggatata aggaatctgg catttctggg gattgcttat
    12481 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
    12541 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
    12601 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
    12661 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
    12721 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
    12781 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
    12841 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
    12901 atcatgcaag ctggtggctg gaccaatgta aatattgtca tgaactatat ccgtaacctg
    12961 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgatctcga gccatctgct
    13021 ggagacatga gagctgccaa cctttggcca agcccgctca tgatcaaacg ctctaagaag
    13081 aacagcctgg ccttgtccct gacggccgac cagatggtca gtgccttgtt ggatgctgag
    13141 ccccccatac tctattccga gtatgatcct accagaccct tcagtgaagc ttcgatgatg
    13201 ggcttactga ccaacctggc agacagggag ctggttcaca tgatcaactg ggcgaagagg
    13261 gtgccaggct ttgtggattt gaccctccat gatcaggtcc accttctaga atgtgcctgg
    13321 ctagagatcc tgatgattgg tctcgtctgg cgctccatgg agcacccagt gaagctactg
    13381 tttgctccta acttgctctt ggacaggaac cagggaaaat gtgtagaggg catggtggag
    13441 atcttcgaca tgctgctggc tacatcatct cggttccgca tgatgaatct gcagggagag
    13501 gagtttgtgt gcctcaaatc tattattttg cttaattctg gagtgtacac atttctgtcc
    13561 agcaccctga agtctctgga agagaaggac catatccacc gagtcctgga caagatcaca
    13621 gacactttga tccacctgat ggccaaggca ggcctgaccc tgcagcagca gcaccagcgg
    13681 ctggcccagc tcctcctcat cctctcccac atcaggcaca tgagtaacaa aggcatggag
    13741 catctgtaca gcatgaagtg caagaacgtg gtgcccctct atgacctgct gctggaggcg
    13801 gcggacgccc accgcctaca tgcgcccact agccgtggag gggcatccgt ggaggagacg
    13861 gaccaaagcc acttggccac tgcgggctct acttcatcgc attccttgca aaagtattac
    13921 atcacggggg aggcagaggg tttccctgcc acagcttgat gaagatctga gctcttaatt
    13981 aatgatcata atcagccata tcacatctgt agaggtttta cttgctttaa aaaacctccc
    14041 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
    14101 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
    14161 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
    14221 gatccgttta aactaacttt aaataattgg cattatttaa agttacatat ggcggccaat
    14281 tcgccttctg caggagcgta cagaacccag ggccctggca cccgtgcaga ccctggccca
    14341 ccccacctgg gcgctcagtg cccaagagat gtccacacct aggatgtccc gcggtgggtg
    14401 gggggcccga gagacgggca ggccgggggc aggcctggcc atgcggggcc gaaccgggca
    14461 ctgcccagcg tgggcgcggg ggccacggcg cgcgccccca gcccccgggc ccagcacccc
    14521 aaggcggcca acgccaaaac tctccctcct cctcttcctc aatctcgctc tcgctctttt
    14581 tttttttcgc aaaaggaggg gagagggggt aaaaaaatgc tgcactgtgc ggcgaagccg
    14641 gtgagtgagc ggcgcggggc caatcagcgt gcgccgttcc gaaagttgcc ttttatggct
    14701 cgagcggccg cggcggcgcc ctataaaacc cagcggcgcg acgcgccacc accgccgaga
    14761 ccgcgtccgc cccgcgagca cagagcctcg cctttgccga tcctctagag tcgagatccg
    14821 ccgccaccat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
    14881 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
    14941 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
    15001 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
    15061 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
    15121 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
    15181 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
    15241 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
    15301 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca
    15361 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
    15421 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gccgaccgct
    15481 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
    15541 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
    15601 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
    15661 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
    15721 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
    15781 gttcttcgcc caccccccgg atctaagctc tagataagta atgatcataa tcagccatat
    15841 cacatctgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
    15901 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
    15961 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
    16021 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccctaact ttaaataatg
    16081 ccaattattt aaagttagga attaattcac ccagctttct tgtacaaagt ggttgatatc
    16141 tctatagtcg cagtaggcgg gggggggggg tactggttag ttcataatgt tgttctacct
    16201 atagggttgc agatcccttt agctccttgg gtactttctc tagctcctcc attggaggcc
    16261 ctgtgatcta tccaatagat gactgtgagc atccacttct gtgtttgcca ggcaccggca
    16321 tagcctcaaa agagacagct atatcagggt cctttcagca aaatcttgct ggcgtatgca
    16381 atggtgtctg tgtttaaagg ctgattatgg gatggatccc cgggtgtggc agtctctaga
    16441 tggtccatcc tttcatctca gctccaaact ttgtctctat aactccttcc aaaaactatc
    16501 ttttagcatt agctacagtt taaagcacca cagccttttc tcggcatatt ggattgtatg
    16561 atgctttgtt tattgagaga aatgtgttct gaatgctaca taaaggatgc acagacaaga
    16621 ttttagcatt gccaccataa agggctcaag gtttcttatt ctgcgatgta caaataaaaa
    16681 aatggaaata ggcatctttc tacagatcta cagcattgtg ctttcctcag aacctgatgt
    16741 gatggtaatt cttttttctt tttctcagtt taagaccaac cctccagctg tgactttcga
    16801 gctaacggga gagacagatg gtatatttaa gatagaaaag gatggacttc tgtatcacac
    16861 aagagccctg gacagagaaa caagagcggt tcatcatctg caggtgagct gagacagtgg
    16921 gatgacacac agtgggatga cactcagtgg gatgacatgc agtgggacaa cacacagtgg
    16981 gatgacacac aggcttctgt agcacgctca gttcccgaat cccctgggac cattatccct
    17041 tgcttcttgt tcagttctcc tggcattagt acaaaactgc agtcctcata tgacctccct
    17101 ccctccctcc cttcctccct ccctccctcc ctccctccct ccctccctcc ctcctcctct
    17161 ttcccccttt ccttttcccc tcattctcct cgcctctttg aaaccgacta cagagtgatt
    17221 tttgaccata gtaatccaat ttacatgatc aaagtcctca gatggccata ctcatttggg
    17281 tactggggct gaagcccagc actgtgctcc tggtgtgccc tggtgcttgg tatgtgtgcc
    17341 tcacacccac tatgaaggca gcatcagtgc tgcgcagagc cactgcggcc ccagaatgca
    17401 gaaggaactc atggtcctac tggaaatgct cttcctttcc gtttccccat agcttcctag
    17461 taggtttctg aatagctaga aaggcagagc gctaactgaa aaagagcaag ctgaaaccat
    17521 cttcactttc tgccatgggc tctgtagact gaccactgtg ccagctctga ggagtcatag
    17581 tacaccatca cctgagtaat gttctgcatc cagtaccctg aacatctaga aagctactgc
    17641 catcatcagg gtagcttaac tgattggacc cttattttag cagtatataa tttgggcttt
    17701 tgtgggttct tcatctcctg cagtcatcag gtcattggtc ttttgaattt taagttccta
    17761 taagtgactt tggggcatgt atcttaagtc cagagtcaca aggtagcttt cttttcttca
    17821 acaacagctt gcagccttgg attctcatgg agctatagtg gacggtccag tccccatcac
    17881 catagaagtc aaggacatca atgacaaccg acccacgttt ctccagtcaa aatatgaagg
    17941 ctcagtgagg cagaactctc gcccaggtag aacagtcagt ctgagtatgt ctgtggggtg
    18001 atgaaagagt ggaattgtgt ggctgacagt tgtcactata gctccacaag actgactatc
    18061 taatgtaaat ttcatagcga tctgaatgct gttttattgt gccaattgtt ttggaccaaa
    18121 gtctctcatg agatggcgca acgcaattaa tgataacttc gtatagcata cattatacga
    18181 agttatggtc tgagctcgcc atcagttcat cttgctgagt atctcataaa ttgcctttga
    18241 ctttggcttt aaggaattca ttggttttgt ttttgttttt tttttttttt tgttgttgtt
    18301 gttttgcctc tgtggacacc cacatttgtg tgcccatact cctcccctca attaaaaatg
    18361 gagtaaatca ttgaaagagt ctctagatct tgagaaatgt acagtgtaag tcaaaactat
    18421 ggttctctag tgaactttca ctaaatacca cacagcttga ggacatcgaa ggactgtctg
    18481 gaaaaatgag cttgctctcc aatatccttg tgggatactt agagttactg ttggctcctt
    18541 tccttattgc tgttccccag ctgtggcctg tgccttctct atgttgacct actctctatt
    18601 tctagatcct tgatcttttc atcaacttat aacaaggaat catttgtgtt attcaacccc
    18661 tttcttttct cagtctccag taggcaagta accattcagg gatggctgca ctgtccctgt
    18721 cccttgtccc tagtggcatc cttgtcagtg tttagtatct gcttctcctg ggtgagtggg
    18781 aagaagtgtg gtttgttccc tctgcaagct ttcatggtat aactatttac catggcctat
    18841 ttcaaattac tagtgatatt aatacatgac tttatagact ttgggtctgg ttggttttag
    18901 catatggtgt ttttatttgt atgcttttct tgtaatcaaa tttatacaaa ctttagaaaa
    18961 ataactgact gaaaaatttc tggaaattca aaattgtgct ctcccaaggt gagtccagtg
    19021 tagcactggc taacatcgta gaaagtaaaa cactgactta gtgaataatt taaaagagca
    19081 cagagtgttt agtgaaaagt atatttttca agacaaaaat agtttaatga gaaaaatgac
    19141 atcatttgaa tctttgcaag tttcttaaat gtctggatga ataaaaacaa ttgggataaa
    19201 ggtctgtgtc tggatccatg atgttcccac atgctgtttt tgttgaagta gtatgtgacg
    19261 aaaattagtc tcacacagat ctgtagctta ggaaatgagg aatattttaa cagcatcttc
    19321 agatattatg gacattcttt catatacaac caaagcttga aaaatgcatt tttcttgcag
    19381 atggtagcaa tataataatg gtatattttg tactatttta ctttaaaatc cactaaatta
    19441 tcttgttctt aaatggacct tttctagatg tgatttcaaa caccataact ttttgaaata
    19501 ttcctgagga ctcctatagg atacaaaata aagattaatt tttccagtta ggaactgagg
    19561 caatttcctc cactctcttt gcatctcctg aggcagacat ggatacagtt gctttaagtt
    19621 tcaactctct tctgcgtatg ctttgtcacc aggaaagcct ttcatgtatg tcaatgctac
    19681 agatctggat gatccggcta ctcccaatgg ccagcttttt tatcaaattg tcatccaact
    19741 tccccaaatc aacgatgtta tgtactttca aatcgacagc aaaacagggg caatatcgct
    19801 taccccagaa ggtaagtcaa ggcaagccct cacaaacctc agccagagga aggtctgtca
    19861 cctgtgggca catgtctttt ctccttctcc tccccaactt ctacaggatc ccaggaattg
    19921 gatccagtga agaatccttc ctacaacctg gtggtctcgg tgaaggacat ggggggccag
    19981 agtgagaatt ccttcagtga tactacatat gtagatattt ctattagaga gaatatctgg
    20041 aaagcaccag aacccgtgga gattagagaa aactcgactg atcctcaccc catcaaaatc
    20101 actcaggtag tgtccaagtg acatttattg ctagttcgga tccatacctt gatatgtaga
    20161 gacatatttt gtggtcttca ctaattgttt tactagatag agttttgcat catataatta
    20221 caaagcaaac taagtaatct ccaaagatta tcatcacaag cagaaaactt ggcccatgtg
    20281 cacaaacgcg cgcacacaca cacacacaca cacacacaca cacacacaca ctttattgtg
    20341 gtgttttatt gttttcattc agcagtgtga aatatagctg ctttatagta ggttgaatgg
    20401 catgtggtat accataaata tatgttgtta gtaataatat cagagaaacc aggccttgtc
    20461 tatgtatcag gccttacttt tgaatatttt ttgttgttgt ttggaggata actaggggtc
    20521 agtagttatt tttatttctg agatctttca gcctttaata tttgagggag tacccctcat
    20581 catgtgtctc aactaaatga atgaatgaat gagtctaagt cagttgttct caacctttgg
    20641 gggttgaatg accctttcat aggggtcaca ttggatatcc tgcttatcag atatttacat
    20701 tgcgatccat agcagtaaca aaattacagc tatttaagta gcaataagaa taattttatg
    20761 gttggggtta ccaaaacatg aggaattgta ttaaagggtc tcagcattag gaaggtttag
    20821 aactacctct ttaaggaatc ctttattgct ctggattcat cttaattttg gattctagaa
    20881 gctcatcata catcatatga agtgcctttt ttcttgataa attgatgtta tacccagtca
    20941 gagttctaga agaaaacaac ctgtgggctc tttgtgagtt tataaagatt gctttaagga
    21001 agaaagaatt cccttgtctg cttttaaggt tagtttaggt tgagaatttt aaatatgtta
    21061 ctcagttgcc ttaagaaact gctcatgtgc agacagagtt gtgagaaatt ccaaagtgag
    21121 aatatagaag ctgttgatca ccttttcagt ctgttaattc ttgtgttttt tcccaccact
    21181 cgctttggcc gtgcaggtgc agtggaatga cccaggggcc cagtattcct tagtcaacaa
    21241 ggagaagctg tcgccgttcc cattctcgat cgaccaagaa ggaaatattt atgtgactca
    21301 ggccttggac cgggaggaaa agaactcagt gagtgagacg ggatgttccg tgggccacaa
    21361 gacaaatcct atgtctactt acaattgagg acaaattaat tgtaaattat taaaaaaaaa
    21421 tggaacaaaa ctgtgttcta gatcctggat gtttgtgcat tctcaaagac acagtgattc
    21481 gataatttcc ccacaagtgt agccaacata cttagaatgg agaattgagg ggggagtaag
    21541 tatgtctgtg ggatgatgaa attcactccc cccccttcat ttctcaattc taaacatgtt
    21601 gacactaagg tgagagatgg ttagcaaaac agttattttc ctccacctgt taaagtgaag
    21661 gcatcttctt acctttgacc ttcacattgc agcatgtttt ctttgcaact gccaaggatg
    21721 agaatggaaa accacttgca tatccactgg aaatttatgt gaaagttatt gacattaatg
    21781 acaacccacc gacatgtctg tctccagtga ctgtatttga agtccaggag aatgaaccat
    21841 tgggtaagaa atgagagctg tttgatgtca tgaacctgcc ttcctcagct gctcctgtta
    21901 tccctttcta gcctctgggg ggtcaggtag acctattctg cagtccattg tccttgtcac
    21961 agagcttgaa ggtatcatga aagtcatata ggtacaacac tttgaaatga acactgtctc
    22021 tggtcctctt aagtttgatg cttttataac tgccaccacc accactgttt caatgacagt
    22081 cactgcagag cagcatcttg tgtacaagtc cagaagggtc cttgttgcaa gattttccct
    22141 tcagatatct tatcatgctt ctaaacaaaa tactttcaag agataaaacc tcatccagca
    22201 tgcta
//