LOCUS       240688#pL1L2_GT1_LF2A_nEGFPO_T2A_CreERT_puro#L3L4_pD223_DTA_T_spec        22884 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT1_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 240688
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000028826
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            4275..4297
                     /db_xref="ENSEMBL:ENSMUSE00000253481"
                     /rank="2"
                     /note="ENSMUSE00000253481 3' fragment"
     exon            4720..4813
                     /db_xref="ENSEMBL:ENSMUSE00000168664"
                     /rank="3"
                     /note="ENSMUSE00000168664"
     misc_feature    4275..10125
                     /note="5 arm"
     misc_feature    10153..10177
                     /note="B1 Gateway"
     misc_feature    10190..10237
                     /note="Frt"
     misc_feature    10238..10737
                     /note="mouse En2 intron"
     primer          complement(10306..10327)
                     /note="LRPCR LAR3_EN2"
     primer          complement(10202..10225)
                     /note="FRT3"
     primer          14403..14421
                     /note="seq primer NF"
     misc_feature    14463..14470
                     /note="RE sites"
     misc_feature    16263..16296
                     /note="loxP"
     misc_feature    complement(14471..14502)
                     /note="Rox"
     misc_feature    complement(16223..16254)
                     /note="Rox"
     misc_feature    14503..14524
                     /note="polylinker"
     CDS             15083..15679
                     /note="puromycin N-acetyl transferase"
     misc_feature    15198..15203
                     /note="Mutated RsrII site"
     intron          10738..11112
                     /note="mouse En2 intron"
     misc_feature    11110..11112
                     /note="Splice Acceptor"
     exon            11113..11166
                     /note="mouse En2 exon"
     primer          11013..11034
                     /note="seq primer FCHK2"
     misc_feature    11167..11289
                     /note="1Dleader"
     misc_feature    11290..11341
                     /note="F2A"
     misc_feature    11437..12153
                     /note="EGFP"
     misc_feature    11347..11436
                     /note="3X NLS"
     misc_feature    14219..14457
                     /note="SV40 polyadenylation site"
     misc_feature    13252..14196
                     /note="ERT2"
     promoter        14535..15046
                     /note="PGK promoter"
     misc_feature    15790..16222
                     /note="PGK pA"
     primer          14277..14300
                     /note="LRPCR RAF5"
     misc_feature    12163..12216
                     /note="T2A"
     misc_feature    12213..12213
                     /note="A to G to kill RsRII site"
     misc_feature    12217..13242
                     /note="Cre"
     misc_feature    11128..11167
                     /note="FCHK lf2a_lacz_gt1"
     misc_feature    11128..11143
                     /note="FCHK QC prefix region"
     misc_feature    11144..11161
                     /note="FCHK QC critical region"
     misc_feature    11162..11167
                     /note="FCHK QC suffix region"
     misc_feature    16319..16339
                     /note="B2 Gateway"
     misc_feature    10126..16369
                     /note="Synthetic Cassette"
     exon            16742..18507
                     /db_xref="ENSEMBL:ENSMUSE00000168665"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 1 ENSMUSE00000168665"
     misc_feature    16370..18656
                     /note="Critical Region"
     misc_feature    18680..18713
                     /note="Downstream LoxP"
     misc_feature    18657..18736
                     /note="synthetic loxP region"
     exon            19210..19537
                     /db_xref="ENSEMBL:ENSMUSE00000253457"
                     /rank="5"
                     /note="ENSMUSE00000253457"
     misc_feature    18737..22884
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggaacaggg atcacaatga agaaatggta agttgagatg ttggtcttga
     4321 gtcttgactt gcatgtacac cttagactgt ctcactgcct ttgacagctc cctccctcat
     4381 gtcttgaaag gccaaagttt tgtccttcag agactgggac aaatgatggc cctcctgggg
     4441 gcgggattta gtcttcctga ggacagaggc aatttccctc taagctgctt agaagtgggt
     4501 ttctatttcc ctttgggcta tcaccaccca atgccttctt tcattctgta gtgtagtgcc
     4561 tgggaggctt cctcagggtc ctgtaagtct gggtccctgc cctgagcatc ctacatcagt
     4621 tctagtcaca ttctcaagcc gccgaagtca caggcgctcc tggtcaagct gtgtttcagc
     4681 gacaccagct ttcccgccca cttttgtgtc ttctcatagg tgactcgctg cattatcgaa
     4741 gtcctctcaa atgccctatc caagtccagt gttccaacga tcacccctga gtgccggcaa
     4801 gtcctgaaga agagtaagtg tctcataatt cagaaagatc tttgtgcctc cttttcctcc
     4861 agcctccacc ttacctgcag acgcatcctt gtggtataac ttgacaggca gtgttgattt
     4921 actagtatgg tacccaggga gacagtgata tgacttctgc tcccttcgta gctgtctgac
     4981 agtggggaag atcccctttt tccctaggct tttttttttt tttttccaga aaagggaatt
     5041 gagattgact gtgtggtctt tgagctgtcc atcagccctt aaattccttg atttcatatt
     5101 ttaaaaaaaa ttaaaaagag tcttcatttg ggaggagaga ggtgtgggaa gtgcttgcgt
     5161 ggggggtaga taaggaggtg actgaggaag tgttgggaga tgagaggaga gcaaggttga
     5221 aaaactgctc tgaggctagc acagtatttt ccctgtactg aagagctgtg ggaaacacag
     5281 tttaaacagt gacgtaagca gtatctgctg tcaccagttt ctaatggaac tatgcagtcc
     5341 ttgtccttta ggaaggtaag ccgattcaaa caaagtctgc tcctgtcaat accttccact
     5401 gctttgcagt gcttaggcaa gctgggcacg gtgagtgctt tcttttcacc tttaagatct
     5461 accggggctg gagagatggc tcagtggtta agaacactga atgttcttct agaggtcctg
     5521 agttcaattc ccagcaacca catggtggct cacaagtgta ataggatctg atgccctctt
     5581 gtgatgtgtc tgaagacaga gatggtgtac tcatataaaa taaataaata aataaataaa
     5641 taaatcttta aaaaataaag gtctctcatt acaaggcaaa ctctcttgtg gttgtgacac
     5701 atggagctca tgcataaagc agggacaatc gacagagtag cctggattaa ctcaatctgc
     5761 ctcagaattg tggcaggtat gggggtggtc caagctcaag tcaacagtga atggatgctt
     5821 ccctgtgccc ctccaggcaa gatgcttcag tttttctcaa atttatcttc tgcttgaata
     5881 gctagggggt tctaattcat cccaggaaac aaatgctact tctccgggcc aacccacttt
     5941 gcatcacctt ttccctgtct tcatttacta gatggggtca aaaggaaggg cctgtcatat
     6001 aaggtaacca tggttgctca gaggaaagtt accggattcc ttactctgct tgtctcccaa
     6061 agcaggtgct tagccctcag gtaattccaa tttgccagtt gttccctggg atctgtctgt
     6121 tgattcttga ttcagagatc aaaataacag gctcaaatct ctaacaagaa cactgcctag
     6181 acagagtcag aaatggcctg gaagatggac ctgtccttca gggttgcagt ttcaagcccc
     6241 actcaggcta gactcgaaac tatgaaggag caagcaagac taagagtggc cgagaagcag
     6301 cacagtgtgc ctctctcagg gtcagcagtg cgggaatatg ggcctggtct cagtggcagc
     6361 ggcagcagca gcagcagcag ccttagaggg ggtggggaag gctttctcag gagcgcctgc
     6421 tccttgactc atcctctggg gaagaacgac acactctgtc accagacttt gtaattcaga
     6481 tcttgcaaac tttttttttg ttgttttttt gtttttgttt ttccattttt ttttattacg
     6541 tattttcctc aattacattt ccaatgctat cccaaaagtc ccccacaccc tccccccccc
     6601 ccactcccct acccacccat tcccactttt tggccctggc gttcccctgt actggggcat
     6661 atacagtttg cgtgtccaat gggcctctct ttccagtgat ggctgactag gccatctttt
     6721 gatacatatg cagctggaga cacgagctcc ggggtactgg ttagttcata atgttgttcc
     6781 acttataggg ttgcagatcc ctttagcttc ttggatactt tttctagctc ctccattggg
     6841 ggccctgtga tccatccaat agttgactgt gagcatccac ttctgtgtgg ctaggccccg
     6901 gcctagtctc acaagagaca gctatatcac agtccttctt gcaaactttc aaaggaagtt
     6961 aagactttga atttttactt gattttgttt tggtggtggc aaggaacaag cccagttttt
     7021 aaggatttta ccactgactt aaatatcaag ctaattatct atctatctat ctatctatct
     7081 atctatctat ctatctatct atctattacc tatctatcaa tcatctttct ttccatttaa
     7141 ttatttacat atttattttt gagacataat ctcactatgt cttacttatg tagttctggc
     7201 cagcttggaa ttcattatgt aaacaggagt ttagctttga cctcagagac tcacctgcct
     7261 ctgtctccca agtgcaggga ttaaaggtat gcaccaccac accctgctta aaattctgat
     7321 tgtttacagt gacatctgat aattagatgt tgtctctgca gtgtaggtca atgggaactc
     7381 ggttctgggc cacaccattc ctctactgtt gtagctttct gtctcctagc cctagttgtc
     7441 tttcttctta cctgataggc aggcttgtat gctgagctga gtggaaggaa tggcctgcct
     7501 ggtccatgtt ccaccctaag cctcatctcc cactctcctg cccacagtga gcaaataagt
     7561 atttggggag aagggatgaa taatagagtt tgtttcttgt ttgtttggtt tggataggct
     7621 ttggtttgag tatttctttt gcggggaggg tacttaaaga tgctagtcca gtttcttacc
     7681 aggtaaaaga tttttttaaa aaatttcttt tttggattaa cctttttaaa aaaataagta
     7741 aataaataat aagattttat ttatttattt tatatataaa tgacaagtac actgtagcta
     7801 tcctcagaca caccagaaga gggcaccaga tcccattaca gatggtttgt gagccaccat
     7861 gtggttgctg ggaattgaac tcgggacctc tgaaagagca gtcaatgttc ttaaccactg
     7921 agtcatctct ccagccctgg gtacaagatt ttttctggcc agggctgact aaggctaagt
     7981 tgatctggtc aactttgcaa caggatggat aagatatgct ccccgtttaa cctggaggca
     8041 agaatgagtg tcacactttg gtgttgtagc ttgactaaga cttccaaggc tagtcagccc
     8101 tcccccgtct cacctgctgg gcttagagct aaatttagga ggaggtgttc agctccagac
     8161 acaagctgct ataggcagca cacatttaac acagaaggaa aataaaaata cttctgtgct
     8221 tcacacttaa gacacctcac accttaaatc gggtaggaag aggagcatgt ggcttctgct
     8281 gtccttcagc aaactctgtg agtgacatca gcatctgcct ggctgaatca gctgcgagca
     8341 ctacagacaa taccgtagtc acacagggtt aaacaacaga cactaactcc tcaccgctgt
     8401 catagcttgt gtacatgatc aaggtcaggt cccacatctt ggctgtctgg ccatcttctt
     8461 accgtggatg gcctcctggg ttaggggagg tcatgtcttg gctgcagggc cttcttctca
     8521 ctgtaccctc ctgtgtgaag ggagactgtg gctcttatgt gtccctttat taagaccttg
     8581 ctcttattat gagggctcca ctcagggcct aagaaccttc caaggctttc ctccacaaat
     8641 tatcatagca ttgattagta tttcaccata ggagtttgga aagggggcac aaagagtcaa
     8701 gggtaccacc caaagtctcc ttgttttctt ggactccact aattagccct gccccagcac
     8761 tgcatgtaat atcatgggct ttttggtttt ttgtttttca gataaggtct ttgtttgctg
     8821 ctcaggctag tcttaaactc actttgtagc cccagctggc atagaactca taaccctcct
     8881 ggcatcctcc ttggctctta taatgccaca gggactgtaa cagacgtgtg ggtgctcaga
     8941 tgcctgtata tacaagtcca aagacagtcc tgcagccttg taaacatggg agccttatta
     9001 ccatgccaag ctgtgtttta atagagaggt ataagccagc tatggtttct tgaatattgt
     9061 ataaaataga taaatgttgt cctttgtaaa tatcagctca aaatgactca tgggatctgt
     9121 ccctttatgc tttcttgctt gcgttctctt aagctccctc ctgttaacat ataataaact
     9181 cctgtctaaa aacaaacaaa tagaaccatc aatgctgttc agaagaaaag tagcatgatc
     9241 tacaaatata atttcaaatt ttctagtgga gatatttaaa gttataaaaa aatacaaatg
     9301 aaattaatgt caatagtcta tctcatttta tctaatgtat tttatccact cagtatattt
     9361 aatattatcc aatctattga aaacgttact ttaacaatac tctttcttcg tatgaagtct
     9421 ttggtatctg gtgtgtctat catgtctcag tttgctcaag tcacatctca aatgctccac
     9481 agcgacaaag agctagaggc ttccacagtg ggtggttctg atgttgatcc ttagtgcccg
     9541 gtggccctgg cttccagtct cctatctgaa tttggtgaga gaggttgaaa cctaagcctc
     9601 ctcaactaac aacaaagaca gtgtgagctc cagatccctc ttcataggga tcttatggag
     9661 gatgtagtaa agggcttatt gacattggga aaagacggaa gagagcagca aatgccagtt
     9721 atgtcatctg ctactgactc agaaaacaca gaggcagccc agcgtactga aacttattct
     9781 caacatcaaa gaaggtgtgg cctgggtaga cctgagtgga gagagagaga cagagagaca
     9841 gagagacaga gagactttcc attcttatca ctgggtgcaa ccaggcacta tttagtgatt
     9901 ctgagaagag catcattggg ctatcttgtt gctgtacaaa catcattaag tatacagtaa
     9961 gaaaccaatg gggctctggc atcactacct gagccgatga tgtgggacca ctgtgaagtt
    10021 cacagttcat tataggatga aatgcacaat ccatgactga gctaagttga gcagccattt
    10081 cctgttggat agggtccaca caattgatta gctaacaaag tctggaaggc gcataacgat
    10141 accacgatat caacaagttt gtacaaaaaa gcaggctggc gccggaaccg aagttcctat
    10201 tccgaagttc ctattctcta gaaagtatag gaacttcgaa ccctttccca caccaccctc
    10261 cacacttgcc ccaaacactg ccaactatgt aggaggaagg ggttgggact aacagaagaa
    10321 cccgttgtgg ggaagctgtt gggagggtca ctttatgttc ttgcccaagg tcagttgggt
    10381 ggcctgcttc tgatgaggtg gtcccaaggt ctggggtaga aggtgagagg gacaggccac
    10441 caaggtcagc cccccccccc tatcccatag gagccaggtc cctctcctgg acaggaagac
    10501 tgaaggggag atgccagaga ctcagtgaag cctggggtac cctattggag tccttcaagg
    10561 aaacaaactt ggcctcacca ggcctcagcc ttggctcctc ctgggaactc tactgccctt
    10621 gggatcccct tgtagttgtg ggttacatag gaagggggac gggattcccc ttgactggct
    10681 agcctactct tttcttcagt cttctccatc tcctctcacc tgtctctcga ccctttccct
    10741 aggatagact tggaaaaaga taaggggaga aaacaaatgc aaacgaggcc agaaagattt
    10801 tggctgggca ttccttccgc tagcttttat tgggatcccc tagtttgtga taggcctttt
    10861 agctacatct gccaatccat ctcattttca cacacacaca caccactttc cttctggtca
    10921 gtgggcacat gtccagcctc aagtttatat caccaccccc aatgcccaac acttgtatgg
    10981 ccttgggcgg gtcatccccc cccccacccc cagtatctgc aacctcaagc tagcttgggt
    11041 gcgttggttg tggataagta gctagactcc agcaaccagt aacctctgcc ctttctcctc
    11101 catgacaacc aggctcccag gtcccgaaaa ccaaagaaga agaacgcaga tcgcagatcg
    11161 cagatctcta gagtcaccga gttgctttac cggatgaaga gggccgaaac atactgtcca
    11221 aggcccttgc tggcaatcca cccaactgaa gccagacaca aacagaaaat tgtggcaccg
    11281 gtgaaacaga ctttgaattt tgaccttctc aagttggcgg gagacgtcga gtccaacccc
    11341 ggaccggtgc atgtggaccc caagaaaaag cggaaggtgg accctaaaaa gaaacgcaaa
    11401 gtggatccta agaaaaaaag aaaggtgcac gtgtccatgg tgtccaaggg cgaggaactg
    11461 ttcaccggcg tggtgcccat cctggtggaa ctggatggcg acgtgaacgg ccacaagttc
    11521 agcgtgtccg gcgagggcga aggcgacgcc acatacggaa agctgaccct gaagttcatc
    11581 tgcaccaccg gcaagctgcc cgtgccttgg cctaccctcg tgaccacact gacctacggc
    11641 gtgcagtgct tcagcagata ccccgaccat atgaagcagc acgacttctt caagagcgcc
    11701 atgcccgagg gctacgtgca ggaaagaacc atcttcttta aggacgacgg caactacaag
    11761 accagggccg aagtgaagtt cgagggcgac accctcgtga acagaatcga gctgaagggc
    11821 atcgacttca aagaggacgg caacatcctg ggccacaagc tggagtacaa ctacaacagc
    11881 cacaacgtgt acatcatggc cgacaagcag aaaaacggca tcaaagtgaa cttcaagatc
    11941 cggcacaaca tcgaggacgg ctccgtgcag ctggccgacc actaccagca gaacacccct
    12001 atcggcgacg gccctgtgct gctgcctgac aaccactacc tgagcaccca gagcgccctg
    12061 agcaaggacc ccaacgagaa gagggaccac atggtgctgc tggaattcgt gaccgccgct
    12121 ggcatcaccc tgggcatgga cgagctgtac aagcctgcag gcgagggcag aggaagtctt
    12181 ctaacatgcg gtgacgtgga ggagaatccc gggccgtcca atttactgac cgtacaccaa
    12241 aatttgcctg cattaccggt cgatgcaacg agtgatgagg ttcgcaagaa cctgatggac
    12301 atgttcaggg atcgccaggc gttttctgag catacctgga aaatgcttct gtccgtttgc
    12361 cggtcgtggg cggcatggtg caagttgaat aaccggaaat ggtttcccgc agaacctgaa
    12421 gatgttcgcg attatcttct atatcttcag gcgcgcggtc tggcagtaaa aactatccag
    12481 caacatttgg gccagctaaa catgcttcat cgtcggtctg ggctgccacg accaagtgac
    12541 agcaatgctg tttcactggt tatgcggcgg atccgaaaag aaaacgttga tgccggtgaa
    12601 cgtgcaaaac aggctctagc gttcgaacgc actgatttcg accaggttcg ttcactcatg
    12661 gaaaatagcg accgctgcca ggatataagg aatctggcat ttctggggat tgcttataac
    12721 accctgttac gtatagccga aattgccagg atcagggtta aagatatctc acgtactgac
    12781 ggtgggagaa tgttaatcca tattggcaga acgaaaacgc tggttagcac cgcaggtgta
    12841 gagaaggcac ttagcctggg ggtaactaaa ctggtcgagc gatggatttc cgtctctggt
    12901 gtagctgatg atccgaataa ctacctgttt tgccgggtca gaaaaaatgg tgttgccgcg
    12961 ccatctgcca ccagccagct atcaactcgc gccctggaag ggatttttga agcaactcat
    13021 cgattgattt acggcgctaa ggatgactct ggtcagagat acctggcctg gtctggacac
    13081 agtgcccgtg tcggagccgc gcgagatatg gcccgcgctg gagtttcaat accggagatc
    13141 atgcaagctg gtggctggac caatgtaaat attgtcatga actatatccg taacctggat
    13201 agtgaaacag gggcaatggt gcgcctgctg gaagatggcg atctcgagcc atctgctgga
    13261 gacatgagag ctgccaacct ttggccaagc ccgctcatga tcaaacgctc taagaagaac
    13321 agcctggcct tgtccctgac ggccgaccag atggtcagtg ccttgttgga tgctgagccc
    13381 cccatactct attccgagta tgatcctacc agacccttca gtgaagcttc gatgatgggc
    13441 ttactgacca acctggcaga cagggagctg gttcacatga tcaactgggc gaagagggtg
    13501 ccaggctttg tggatttgac cctccatgat caggtccacc ttctagaatg tgcctggcta
    13561 gagatcctga tgattggtct cgtctggcgc tccatggagc acccagtgaa gctactgttt
    13621 gctcctaact tgctcttgga caggaaccag ggaaaatgtg tagagggcat ggtggagatc
    13681 ttcgacatgc tgctggctac atcatctcgg ttccgcatga tgaatctgca gggagaggag
    13741 tttgtgtgcc tcaaatctat tattttgctt aattctggag tgtacacatt tctgtccagc
    13801 accctgaagt ctctggaaga gaaggaccat atccaccgag tcctggacaa gatcacagac
    13861 actttgatcc acctgatggc caaggcaggc ctgaccctgc agcagcagca ccagcggctg
    13921 gcccagctcc tcctcatcct ctcccacatc aggcacatga gtaacaaagg catggagcat
    13981 ctgtacagca tgaagtgcaa gaacgtggtg cccctctatg acctgctgct ggaggcggcg
    14041 gacgcccacc gcctacatgc gcccactagc cgtggagggg catccgtgga ggagacggac
    14101 caaagccact tggccactgc gggctctact tcatcgcatt ccttgcaaaa gtattacatc
    14161 acgggggagg cagagggttt ccctgccaca gcttgatgaa gatctgagct cttaattaat
    14221 gatcataatc agccatatca catctgtaga ggttttactt gctttaaaaa acctcccaca
    14281 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
    14341 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
    14401 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
    14461 ccgtttaaac taactttaaa taattggcat tatttaaagt tacatatggc ggccgcccta
    14521 aggctcgacc tcgaaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc
    14581 atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac
    14641 acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc
    14701 accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag
    14761 gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga
    14821 gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt
    14881 ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg
    14941 cggggcgggc gcccgaaggt cttccggagg cccggcattc tgcacgcttc aaaagcgcac
    15001 gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca tccatctaga
    15061 tctcgagcag ctgaagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg
    15121 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
    15181 gcgccacacc gtcgatccag atcgccacat cgagcgggtc accgagctgc aagaactctt
    15241 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt
    15301 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
    15361 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
    15421 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
    15481 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
    15541 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
    15601 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
    15661 gacccgcaag cccggtgcct gacgcccgcc ccacgacccg cagcgcccga ccgaaaggag
    15721 cgcacgaccc catgcatcga tgatatcaga tccccgggat gcagaaattg atgatctatt
    15781 aaacaataaa gatgtccact aaaatggaag tttttcctgt catactttgt taagaagggt
    15841 gagaacagag tacctacatt ttgaatggaa ggattggagc tacgggggtg ggggtggggt
    15901 gggattagat aaatgcntgc tctttactga aggctcttta ctattgcttt atgataatgt
    15961 ttcatagttg gatatcataa tttaaacaag caaaaccaaa ttaagggcca gctcattcct
    16021 cccactcatg atctatagat ctatagatct ctcgtgggat cattgttttt ctcttgattc
    16081 ccactttgtg gttctaagta ctgtggtttc caaatgtgtc agtttcatag cctgaagaac
    16141 gagatcagca gcctctgttc cacatacact tcattctcag tattgttttg ccaagttcta
    16201 attccatcag aagctggtcg agtaacttta aataattggc attatttaaa gttaggcgcg
    16261 ccataacttc gtatagcata cattatacga agttatgtcg agatatttaa atattagacc
    16321 cagctttctt gtacaaagtg gttgatatct ctatagtcgc agtaggcgga attatatcat
    16381 tctgagtctc cgtgtctttt atacgtgcaa aacaaggagg ctgaaccaga tgaactgtaa
    16441 gagttctcct gattttaaat tccagtcttt cctaaagcta catatgggtt tcagtttgac
    16501 cattggcagt tacaaataga gataataggt attattaaaa cctttaaaca tgtagagagc
    16561 tcattagatt taaacacagt caaagagctt ccttccatag catcagcttt tcaaagaatt
    16621 ccatacatcc tctattatgt tcctcttttc cagggtcctc ggatctgtag tgaccactga
    16681 ggctggtact tgaacctcaa gcacttttgc agttgtgctt actcctatat tttcattata
    16741 ggtggaaaag aggtcaaagg tgaagagaaa ggtgaaaacc agaactcgaa gtttgaagta
    16801 cggttgctaa gagacccagc cgatgcctca ggaacccgct gggcctccag tagggaggat
    16861 gcaggcgctc cagtagaaga ctctcaaggc cagacgaagg taggcaatga gaagtggaca
    16921 gaaggaggag gacacagccg agagggagtg gatgatcaag agagtctccg tccctccaac
    16981 caacaagcgt ccaaagaagc aaagatatac cattcggaag agagggtggg aaaagagagg
    17041 gagaaagaag aaggcaagat ttacccaatg ggggagcaca gggaagatgc tggtgaagag
    17101 aagaaacaca ttgaagattc tggagagaag ccgaacacct tctccaacaa aagaagcgag
    17161 gcttccgcta agaaaaaaga tgagtcagtg gccagagcag atgcacactc catggagctg
    17221 gaggagaaga cacacagtag ggagcaaagc agccaggaga gtggagagga gacaaggagg
    17281 caggagaaac cccaggagct taccgaccag gaccagagcc aggaagaatc ccaggaaggc
    17341 gaggaaggtg aggaaggcga ggaaggcgag gaaggcgagg aagactcagc ctctgaagta
    17401 accaaacgca ggcccagaca ccaccacggg agaagcgggt ccaacaagtc ctcttacgaa
    17461 gggcatcctc tatctgagga aaggagaccc tctcccaagg agtccaagga ggcagatgtg
    17521 gccacagtcc gtttagggga aaaaaggagt caccatctag ctcactacag ggcttcagaa
    17581 gaagaacctg aatatgggga agaatcgaga agctatcggg gactacagta ccggggtaga
    17641 ggaagcgaag aggacagagc cccaaggcct cggagtgagg agagtcagga aagggagtat
    17701 aagagaaacc accccgactc tgaactggaa agcacagcaa acagacatgg tgaagaaacc
    17761 gaggaagaga ggagctacga gggggcaaat ggacgccaac acagaggcag ggggcgggag
    17821 ccaggagccc attctgctct tgacaccaga gaagaaaaaa ggctcttgga tgaagggcac
    17881 taccctgttc gcgaaagccc aatagatacg gcaaaaaggt acccacaaag caaatggcaa
    17941 gagcaggaga aaaactacct caactatggc gaggaagggg accaagggag atggtggcag
    18001 caggaagagc agctaggacc ggaagaaagc agggaggaag taaggtttcc agacagacaa
    18061 tatgagccct atcccatcac tgaaaagagg aagaggttag gggcgctgtt caacccgtac
    18121 tttgaccctc tccagtggaa gaatagtgac tttgagaaaa gaggcaaccc agatgacagt
    18181 tttcttgagg atgaaggtga agacagaaat ggggtgacct tgactgagaa gaattccttc
    18241 ccagagtaca actatgactg gtgggagaga aggcccttct cagaggatgt gaattgggga
    18301 tatgagaaga gaagctttgc cagggcccct cagctcgact tgaaacggca atatgatgga
    18361 gtggccgagt tggaccagct gcttcactac aggaagaagg cagacgaatt tcccgatttc
    18421 tacgactcgg aggagcagat ggggcctcac caggaggcaa acgatgaaaa ggccagggct
    18481 gaccagagag ttctgacggc ggaagaggta cggtttgagg cttttgctta gaattagact
    18541 atgttgatca aggttggttg ttattagcat caacatgtct gataacgggg agagatgagt
    18601 gggacattca ctgtgagaat gtgagtgccc ctgtcataga gcaggattga tttccagaga
    18661 tggcgcaacg caattaatga taacttcgta tagcatacat tatacgaagt tatggtctga
    18721 gctcgccatc agttcatctt ttatagtgtt tagaaaacgt gaaaatctac tgtttctggg
    18781 atcaatcccc tgagtttctg tccgatgagt accggtgctg tctaggtgtg tagtatacct
    18841 tagtgctggg gcaccctaat gcctgccacg tgattcccat gcccatgcca acctcccatt
    18901 cggagccttg acctaattcg atcatgtcag gtggtgcctc tcaagcttga aagtacatca
    18961 gagtcacatg gattgctgat ccctagggtt tctcagtcag caattttata tgggacccag
    19021 gaattcctag gcaattgctt gggttaccca acctactcat catgaaagcc cactttgaag
    19081 acccctgatg ttggctgact caggaagcct ctactgtacc caagctcccc ctttctcctt
    19141 agagcttcct gaactggagt ggtatggtag tgtgacttgt catcaatgct ccacttttct
    19201 gtttcccaga aaaaggaact ggagaacttg gctgcgatgg atctggaact ccagaagata
    19261 gcggaaaagt tcagccagcg gggctgacag ttggagagac gagcctttca ctgaaggaca
    19321 ccattgattc acccacaggc agaaagcaga atttactgtt tattaaatgt ttgatgcagt
    19381 tggaaatatc atttatcttt gccagaatgc taatgccatg tgactagcgt gacttgtagc
    19441 gtattcttcc tgcaaaatag atgtttcacg tgtctgtgac aatgaccgtg ttactgttgt
    19501 tgaaacacaa acaataaaaa gatttacctg aaacaaagtc ctttttggtt cttagctctt
    19561 tgggttagtt aggaagagtg tggttttgaa atccattctt ctaggtatat tctcaaagat
    19621 agaatttgtt ggtctttagc aggaaaaagc aaggtttctg aagtaaatac tgcaggatct
    19681 aaagaaagag attgagaata aaacatggct aatagagacc atataggctt gaagaaccca
    19741 ttttaaaaga aaatgtatta tttaaaattt ttattcattg tgtgttaagg gtttcagagg
    19801 acaaccctca tgagttggtt tactgagata aaaaaaaaaa aaatcaggtc aaatggcttg
    19861 gtagccagtg tcttacccac tgagctattt catcaacgca aaatatttta aaattctgca
    19921 aagtaggaga aaaggaaaca gctcactata gaaggctata gcatgtataa agctctgggt
    19981 tccatgatca ggacagagaa aggctggaaa aaaaaaaggc tgtccttaag ggggacagta
    20041 caaaccagaa atgcagctgc atctccccac tagctgtctg caatagcctg tatctatagg
    20101 cagctacttg tgaggccaag gcaacagaat agccccagag ctcaaggcca gccagaccca
    20161 tctcaagaga aatacaaata attaggttct tataaactgg agagctctgg gggtagaatg
    20221 tcaagggagg ggctgaaaga aaagccttaa tggaaaaaaa gttctctggt ttgggaatat
    20281 gaacatgtgg tgcaacatcc ctgatacctc cctctgtaca tacttgattt ctaccttgta
    20341 tgcgagcaga gctctggctg ttgagtctga gctgtgtggc ttatagtcag ttgctttccc
    20401 aagttctagt tctcaggctc aataaaataa aggtatcttt ctcacaaggt tatttctaac
    20461 aagagtttat gtgagccact cagtactgtg cccctccccc accctgggaa tcaatggcat
    20521 aggtaatgtt ggccactgct gttatcatct ttgtagcttc ttgttccaag acaatcagac
    20581 aatctcctct ttcctcaatg attgatcatc tcatttctta ccagtggcag cttcaactgg
    20641 gttattttta ttgttgtctg ttcaggtaaa aacctgcttt ctggaaggtg ctaaaaatgt
    20701 ctgagggtcg tgggtgatct ttattaacac aggaagacca gaaggtctcc tcctttgcta
    20761 aatcaaggaa tggtgtacta acttagccat atttgttcca aattaacact gaatgaactg
    20821 agattttatt gtataggaac caagtcacct ggtgctaggt aaatagttct aaacacaggc
    20881 tcccatttca agagagcctc ttactgtctc ttttctgggg tctttcatcc tgcatatggg
    20941 ataaggacca gcggcatgaa agggccaagg gaacatgtac agacctcatg aatgctgaag
    21001 gccgcctccc ttccccccca cccccactgt cctgcaatcc taaatcacag tgttggcagg
    21061 gtcgtgcctc tgcagagtgt ctgcagagaa ggctcctcct tgcctcttga gctctgatgg
    21121 tccctgacat ttcttggctg tggaataggt aacggtattg gtaatgacag gatcacatca
    21181 aagtccaccc gctcttcctt ctcttcccac cttctccttg caactttcca tctccccaca
    21241 tggcactatt gatgtgaagg aacagcagtc atcacgtact gaagtactga aggtctgtct
    21301 gactccagtg tgacttcatc ataaccaatt gcatccatag taaccctctg tccaagtaac
    21361 acacactttg cggtgtgggg ggtggggtgg gggggtgggg tggggtgggt gttgagacat
    21421 ccgcgtatct ttttctatgc aaacaattca aagaatagta agacaggtca gcagctctta
    21481 cctcctctga gtaggtcatt ctgtggcaca ttctatactg cctcctggaa tgtcccagga
    21541 gcaacctgct tgataatgca tcctttgttg gctgcctttc ccacgttagt agagtgctta
    21601 ctggtaagct tggaagtagt tccatgtaac tatatatcct cacattctaa tgttcggatt
    21661 tgcttctggt agttcatgtc ctaagaaatc aagactcata ttatgaaaat taatctgtta
    21721 tagatcagac atgccttaca cagtgctaag cacacaggct gagctgtgtg tgtgtgtgtg
    21781 tgtgtgttct acgttcttct tctaaccatt tactagcata agagccattt ttatcttgca
    21841 ggttgcatga gttactattt tattgctgtg aaaaaaacac catgaccaag gtgccttatt
    21901 gaaggtgtac tgactagttt tgtgtctcaa cttgacacaa gctatagtca ccagagagga
    21961 aggagtctca gctgaggaaa agcctccaca cacactcaaa tctggtaatt tctgtttaat
    22021 tatacttggc ttacagtact ttgctaaggg agtcctgaaa aaaaatctaa cataacaaga
    22081 aagaaatgag ggttggagag atggctcagc agttaagaac actgactgtt ctttcagagg
    22141 tcctgagttc aattcccagc attcacatgg tgactcacag tcatttgtaa tgcaatccga
    22201 tgcccttttc tggtgtgtct gaagatagct acagtgttct catatacata aataaatctt
    22261 taaaagaaac aagaaatggc tgtaaggcat tttctcaact aatgatcagt ggtggagagc
    22321 ccatcccatt gggggtggta ccatctctgg gttggtggtc ttgagatcta taagaaatca
    22381 ggctgagcaa gccaggggag caagccagta agcagcacct ctccatggtc tttgtatcag
    22441 ctcctgcctc caggattctg ctctgttttg agttcctgtc ctgacttcct tcagtgatga
    22501 acagcaatgt ggaagtgcaa gcaaaaataa acctccctag gtgttttatt gcagcaatga
    22561 taatgctaac taggacagaa ggtaagggtt atttgggctt gtggtttgga acaaaagccc
    22621 atcaccatca cagtggggga gcatgggaga gtggagttga aagctgacag ctgagggctc
    22681 acatttcaaa ccacaaatat tgaaagcaag ctgggcatta ctagtggctt ttaaacctca
    22741 aagcccaccc ccagtgacac acttcctcca gcaaggttat acctcctgac ccttctcaaa
    22801 tagtatcact aggaccaggt atacaaatgt ctgagaatat gcgatatcta attcaaacca
    22861 ccatatgaaa aactagttgt ggtc
//