LOCUS       209375#pL1L2_GT2_LF2A_nEGFPO_T2A_CreERT_puro#L3L4_pD223_DTA_T_spec        21512 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT2_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 209375
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000086521
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            9003..9102
                     /db_xref="ENSEMBL:ENSMUSE00001047106"
                     /rank="7"
                     /note="ENSMUSE00001047106"
     exon            8512..8721
                     /db_xref="ENSEMBL:ENSMUSE00001082376"
                     /rank="6"
                     /note="ENSMUSE00001082376"
     exon            8090..8185
                     /db_xref="ENSEMBL:ENSMUSE00000968196"
                     /rank="5"
                     /note="ENSMUSE00000968196"
     exon            7712..7887
                     /db_xref="ENSEMBL:ENSMUSE00001042165"
                     /rank="4"
                     /note="ENSMUSE00001042165"
     exon            7466..7610
                     /db_xref="ENSEMBL:ENSMUSE00001094993"
                     /rank="3"
                     /note="ENSMUSE00001094993"
     misc_feature    4275..9850
                     /note="5 arm"
     misc_feature    9878..9902
                     /note="B1 Gateway"
     misc_feature    9915..9962
                     /note="Frt"
     misc_feature    9963..10462
                     /note="mouse En2 intron"
     primer          complement(10031..10052)
                     /note="LRPCR LAR3_EN2"
     primer          complement(9927..9950)
                     /note="FRT3"
     primer          14121..14139
                     /note="seq primer NF"
     misc_feature    14181..14188
                     /note="RE sites"
     misc_feature    15981..16014
                     /note="loxP"
     misc_feature    complement(14189..14220)
                     /note="Rox"
     misc_feature    complement(15941..15972)
                     /note="Rox"
     misc_feature    14221..14242
                     /note="polylinker"
     CDS             14801..15397
                     /note="puromycin N-acetyl transferase"
     misc_feature    14916..14921
                     /note="Mutated RsrII site"
     intron          10463..10837
                     /note="mouse En2 intron"
     misc_feature    10835..10837
                     /note="Splice Acceptor"
     exon            10838..10884
                     /note="mouse En2 exon"
     primer          10738..10759
                     /note="seq primer FCHK2"
     misc_feature    10885..11007
                     /note="1Dleader"
     misc_feature    11008..11059
                     /note="F2A"
     misc_feature    11155..11871
                     /note="EGFP"
     misc_feature    11065..11154
                     /note="3X NLS"
     misc_feature    13937..14175
                     /note="SV40 polyadenylation site"
     misc_feature    12970..13914
                     /note="ERT2"
     promoter        14253..14764
                     /note="PGK promoter"
     misc_feature    15508..15940
                     /note="PGK pA"
     primer          13995..14018
                     /note="LRPCR RAF5"
     misc_feature    11881..11934
                     /note="T2A"
     misc_feature    11931..11931
                     /note="A to G to kill RsRII site"
     misc_feature    11935..12960
                     /note="Cre"
     misc_feature    10853..10885
                     /note="FCHK lf2a_lacz_gt2"
     misc_feature    10853..10868
                     /note="FCHK QC prefix region"
     misc_feature    10869..10879
                     /note="FCHK QC critical region"
     misc_feature    10880..10885
                     /note="FCHK QC suffix region"
     misc_feature    16037..16057
                     /note="B2 Gateway"
     misc_feature    9851..16087
                     /note="Synthetic Cassette"
     exon            17509..17638
                     /db_xref="ENSEMBL:ENSMUSE00000983610"
                     /type="targeted"
                     /rank="10"
                     /note="target exon 1 ENSMUSE00000983610"
     exon            16991..17127
                     /db_xref="ENSEMBL:ENSMUSE00000997429"
                     /type="targeted"
                     /rank="9"
                     /note="target exon 2 ENSMUSE00000997429"
     exon            16482..16657
                     /db_xref="ENSEMBL:ENSMUSE00000985197"
                     /type="targeted"
                     /rank="8"
                     /note="target exon 3 ENSMUSE00000985197"
     misc_feature    16088..17794
                     /note="Critical Region"
     misc_feature    17818..17851
                     /note="Downstream LoxP"
     misc_feature    17795..17874
                     /note="synthetic loxP region"
     exon            21018..21176
                     /db_xref="ENSEMBL:ENSMUSE00000970032"
                     /rank="15"
                     /note="ENSMUSE00000970032"
     exon            20444..20564
                     /db_xref="ENSEMBL:ENSMUSE00001080918"
                     /rank="14"
                     /note="ENSMUSE00001080918"
     exon            19787..19962
                     /db_xref="ENSEMBL:ENSMUSE00001011445"
                     /rank="13"
                     /note="ENSMUSE00001011445"
     exon            19412..19539
                     /db_xref="ENSEMBL:ENSMUSE00001054392"
                     /rank="12"
                     /note="ENSMUSE00001054392"
     exon            19154..19304
                     /db_xref="ENSEMBL:ENSMUSE00001085497"
                     /rank="11"
                     /note="ENSMUSE00001085497"
     misc_feature    17875..21512
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggaagcacc ccttggggaa ctcaaaccca aataggtcag aagacaattg
     4321 ccaaatgcct agggccagga cctgggctgc cacctcccag tatggcccga ttttagatct
     4381 gctccttcct gagacctgga aagtgcttct ctgcaattgg cctgctcaac agatcctagc
     4441 atagatctct gggacactca cacccagggc aaggagaggc gtagcatcct ccctcacaaa
     4501 gcatctctct ggcttctttc ttccactgca gtgtctcatg actcttctgg ggaaaagaat
     4561 aaccggggca gagtgggggc tttgggggca ggagccacag agaaggctgc ctttgccggg
     4621 gagtatgtgt gtccatatgt gctcacctgt atgtacggca gcatagacag gtctgtctgt
     4681 agctacttgg agtagtgtgg gctgtgtgag gcggtctgta gaccagatag aggctatggg
     4741 gtgcgggtgt gagtgcagca catcttggac ctccatgccc tcaactccag ctcctttcag
     4801 aagcatcttt tacacccaga tttctgggtt tcattcccag aacttctgat ttcaggcctc
     4861 cagagtgaag ccagggcctc agatggcccc aaaaacctga agtcaggaac tgtagccact
     4921 gtggttaaaa atgtgggctg caaggcgagg ctggatgagg gcagacctcc tttgctttga
     4981 agtgcagaaa gttattaaac tttctccatg ttggagacat aagaagcacc tcacttctta
     5041 gatcactttg agaaggaatt tagagtatgg tatcaggcag ccatatatgc aaacagatca
     5101 acagcacaat tcttatcatg gcttaattat tatcaatgat acatgcatat gggatgagca
     5161 ggcggggcta ttggcagctc tgcctgcctt atccagaagt gtgtgctgat gcatatctgg
     5221 ccatctgatt ggcttagaga gagacacctg tatatgagca catgtgtgtt gtgagaacat
     5281 tgtacgatca tgtgcaggta agacatgaag gtgtgtacat gtgagtattg gggtgtgcgt
     5341 gagggtgtgc acttgactga gtgtagaaga gacagccttt tcaagatgaa agacaacaat
     5401 cttaatctta actacaggtg tgcccagacc ccactctgcc acctcccacc acccagactg
     5461 agttcctgga agctgctatt gcctacggtg gttgttccca gcagcctctc catccctcct
     5521 tctcctgctt tccatcctcc cctctctcca ggtggtccca gctgggacag ggtccctacc
     5581 ctccatcacc atcattaact cgggattaag ctgggctttc tagggggaga tgatgcagtt
     5641 gctgtcaaac accacctctg aggccccagg cagcagtaag agagagtggc aagcaactgg
     5701 gggaggggca gacgacataa acaggactca agggaagccc ttgccagact gttctgaaag
     5761 tcctgcctcc tacctcattt ctcattcaga gggctgctta gcagatgccc caactcccaa
     5821 gaggccagag ctggccctca gatgcagcag agggaccaca ctcgctcaga agagggctgg
     5881 cagctggatg tgcccagaaa gaaggcacta attggttaat ggagttccct cctcccatcc
     5941 cccacccaac acggaaggtc tgaaatcagc tgcaggagag attggggcca attagggggc
     6001 ctggctccca cctgatctcg cagtggtgga actcccactg tgcctatgcg ggtgtgaagt
     6061 actgcccagg cccctgcctg ctgatcctgt gctggtgaga caggacaatg gtgtgtgcaa
     6121 agcaaggccc tggctgagac agggctggga agggcaagtg tgaaacccat ttgctaagca
     6181 acatcacctg gctgacacag agtgactgga tgaccactgg gcctttcttt cctgagacgg
     6241 gacatggtta taaaactgct accattcgac ttgctaaccc agccaacatc tagctgtcct
     6301 tcacccctga ccccagttga gtctaacagc caggtgctgt ttgcatcctg tgtcaggccc
     6361 tccctatttt tcactgttag accctgttat ggacctggtg ttagcattct gtctaaactc
     6421 caccccacag ctacctggca acagccagat aggcctgacc cattataaaa gggggttgct
     6481 tgccccctcc tttctcttgt tcctcttgct ctcttggtct cactctgtcc cctcgctcct
     6541 tcccctcctt ccccattcct tacccccctc catgtgctca tggccggcct ctacttctct
     6601 aatctttctc tgcctttctc tgcctctgct accctcttga ctcccctcct catgccctga
     6661 ataaactcta ttctatacta taccatatat agtgcggttg atccctcagg gggacaggat
     6721 gccttgacat gggcccgctg agacaccccc ttccccaaca cctcactgcc cctcaatata
     6781 gaacatattc cctctttctt tatcttttta taaacacatt acctgagaag tgaatttcca
     6841 ctcttttcat aggaaaacat cagatcaata gaaaggaggg cctatagctt gtccaaggta
     6901 atatagccag taggagaccc agcccagctc ccagatgagc acttttcact aaactacact
     6961 gccctaggag ggcactggac tccaggaaca cataacactc ttttcctctg tttcctgtcg
     7021 gtgtcttctc atatgcctca gctgtgtgag atttcctgag agtcttaccg aatcttgtcc
     7081 ctaaagcact ctgcaacatg gcaccctgta ctgtccgttc tcaattttct tccctggact
     7141 gcaaagccct ttaaagaaag ggaccgtgtg tctgattcat ttcacagccc agttccaggt
     7201 atacagcaag tgcttaatat gtgactgttg gtgggtgagt gaatatgcca gtgtagattg
     7261 atgggcctgc ccagtctgtt ttaaattaat tttaattaaa ttaaagtttg gttccttaga
     7321 cacattccaa aaccctcaat agccacacat ggtccatagc tacctactgg acgacacagg
     7381 aaagggtgca gccccagtaa gttctgccct tgacattgga cttcgtctca gacattgagg
     7441 ctatagtggt accccctgtt tgcaggatgg agtttttccc agggaacccc agctaccttt
     7501 ggacccgtct ttgaagagca acctgttggc ctgctattcc cagaggagtc tgcagaggat
     7561 caggtgacac tggcgtgccg tgcccgagct agccctccag ccacctatag gtaagagagc
     7621 ggggaggctc cgggcagcca tatggcattc cttgacacca gtcttaccca ttgagatctg
     7681 aagtcccatc cccacttcat cccggctaca ggtggaagat gaacggcaca gagatgaacc
     7741 tggaacctgg ttcccgtcac cagctgatgg ggggcaacct ggtcatcatg agtcccacca
     7801 aagcacagga tgctggtgtc taccagtgcc tagcctctaa cccagtaggc actgtcgtca
     7861 gcaaggaagc tgtcctccgc ttcggctgtg agatccatgg gagccaagct gttgggtgtg
     7921 ggtagacggg gccaggaaaa ggttcccagg aaggacaggt ggcggtagcc tcgagccagg
     7981 ctttcctgcc agcaaatggc atgctagcga ctgcttcttg cccatggtgg aggaactggg
     8041 gcgctgcatg cttgaagaca gttgcctgag ccctcttctc catctccagt tctacaggaa
     8101 ttctccaagg aggagagaga ccccgtgaaa acccacgagg gctggggggt gatgctgccc
     8161 tgtaacccac ctgcccacta cccaggtaag tggggattct gggccagcgc aggactggga
     8221 gggggaatgt accagaaagg acttctggat gctagcatgg gagaggaaaa acagaacagg
     8281 aggtggtaag tcttaacttt caatggctct gcaatttcct agctggactg agagtgttcg
     8341 cagttcatcg tgaggagaga gtcaaagtca tctctcccct ctccaccccc agcccctgat
     8401 cagagctgca agttctcatg agaagacaga gtttgcagca tgtttggggt cttgcccaga
     8461 ttgcctgtga tcccagtaca cccttgtttc ctcagactcc ttcctttcta ggtttgtcct
     8521 accgctggct cctcaacgag ttccccaact tcatcccaac ggatgggcgt cacttcgtgt
     8581 cccagaccac agggaacctg tacatcgccc gcaccaatgc ctcggacctg ggcaactact
     8641 cttgcctggc taccagccac ctggacttct ccaccaagag cgtcttcagc aaattcgcgc
     8701 agctcaacct ggctgctgaa ggtcaggatc tagggtaatg gaggctgaaa gggtcaatag
     8761 gccttggaga aatggtattc taccaagctt aagaattgca gctcagaata tatgaggtcc
     8821 tgctgccggg tctcagatcc ttgccccagg ccccccagaa aaagagaaca tttggagatt
     8881 ccaaatattt atgtggtgat ctgtgaactt tggaaggcat gggccctaat acaaagcacc
     8941 tccctggagc cctttgcatg tggttcacac agtcaatctc tctctcccct ccaccccccc
     9001 agatcctcga ctctttgctc ccagtatcaa agcccggttc cccccagaga cgtacgcact
     9061 ggttgggcag caggtcaccc tggagtgctt tgcctttggg aagtaagtac catcaggaca
     9121 agagtgtcct gggcccaggc agggtttcat ttcaaagccc ctcaagagca ggagggatca
     9181 cagagttaaa gagaacaaag tcaatagact aacagagtcc tggacacaaa acaggcacca
     9241 cccactacct ataaaacaag ttctgccagt gagacatcac atacacacac acacacacac
     9301 acacacactc acacacacac actcacaaac acacactcac tcacacacac acacatacac
     9361 actcactcac acacacacac tcacacacac acactcacac acacacactc acaaacacac
     9421 acactcactc acacacacac acatactcac tcactcacac acactcactc actcacacac
     9481 acactcacac acacacacac actcactcac acacactcac tcacacacac actcacacac
     9541 acactcacac acactcacac acactcacac acacacagat ggaggggctt tataggcatg
     9601 agggagtcat gtggcctccc aacagtaaac agtaaaatcc taagcagttt aaggagcaag
     9661 ccagggctag cagcagaaaa aacagtgtgc cgaccccatc ctgctggttc aaagctcagc
     9721 ttcaacactt gctggctggg agacctcggg caaacaacct cacatctgag gctcagctct
     9781 aggactgacg gatggtcagg gttgacagtg gctaaaggat tcttacaaag atcagttggg
     9841 tccatggata aaggcgcata acgataccac gatatcaaca agtttgtaca aaaaagcagg
     9901 ctggcgccgg aaccgaagtt cctattccga agttcctatt ctctagaaag tataggaact
     9961 tcgaaccctt tcccacacca ccctccacac ttgccccaaa cactgccaac tatgtaggag
    10021 gaaggggttg ggactaacag aagaacccgt tgtggggaag ctgttgggag ggtcacttta
    10081 tgttcttgcc caaggtcagt tgggtggcct gcttctgatg aggtggtccc aaggtctggg
    10141 gtagaaggtg agagggacag gccaccaagg tcagcccccc ccccctatcc cataggagcc
    10201 aggtccctct cctggacagg aagactgaag gggagatgcc agagactcag tgaagcctgg
    10261 ggtaccctat tggagtcctt caaggaaaca aacttggcct caccaggcct cagccttggc
    10321 tcctcctggg aactctactg cccttgggat ccccttgtag ttgtgggtta cataggaagg
    10381 gggacgggat tccccttgac tggctagcct actcttttct tcagtcttct ccatctcctc
    10441 tcacctgtct ctcgaccctt tccctaggat agacttggaa aaagataagg ggagaaaaca
    10501 aatgcaaacg aggccagaaa gattttggct gggcattcct tccgctagct tttattggga
    10561 tcccctagtt tgtgataggc cttttagcta catctgccaa tccatctcat tttcacacac
    10621 acacacacca ctttccttct ggtcagtggg cacatgtcca gcctcaagtt tatatcacca
    10681 cccccaatgc ccaacacttg tatggccttg ggcgggtcat cccccccccc acccccagta
    10741 tctgcaacct caagctagct tgggtgcgtt ggttgtggat aagtagctag actccagcaa
    10801 ccagtaacct ctgccctttc tcctccatga caaccaggct cccaggtccc gaaaaccaaa
    10861 gaagaagaac gcagatcgca gatctctaga gtcaccgagt tgctttaccg gatgaagagg
    10921 gccgaaacat actgtccaag gcccttgctg gcaatccacc caactgaagc cagacacaaa
    10981 cagaaaattg tggcaccggt gaaacagact ttgaattttg accttctcaa gttggcggga
    11041 gacgtcgagt ccaaccccgg accggtgcat gtggacccca agaaaaagcg gaaggtggac
    11101 cctaaaaaga aacgcaaagt ggatcctaag aaaaaaagaa aggtgcacgt gtccatggtg
    11161 tccaagggcg aggaactgtt caccggcgtg gtgcccatcc tggtggaact ggatggcgac
    11221 gtgaacggcc acaagttcag cgtgtccggc gagggcgaag gcgacgccac atacggaaag
    11281 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccttggcc taccctcgtg
    11341 accacactga cctacggcgt gcagtgcttc agcagatacc ccgaccatat gaagcagcac
    11401 gacttcttca agagcgccat gcccgagggc tacgtgcagg aaagaaccat cttctttaag
    11461 gacgacggca actacaagac cagggccgaa gtgaagttcg agggcgacac cctcgtgaac
    11521 agaatcgagc tgaagggcat cgacttcaaa gaggacggca acatcctggg ccacaagctg
    11581 gagtacaact acaacagcca caacgtgtac atcatggccg acaagcagaa aaacggcatc
    11641 aaagtgaact tcaagatccg gcacaacatc gaggacggct ccgtgcagct ggccgaccac
    11701 taccagcaga acacccctat cggcgacggc cctgtgctgc tgcctgacaa ccactacctg
    11761 agcacccaga gcgccctgag caaggacccc aacgagaaga gggaccacat ggtgctgctg
    11821 gaattcgtga ccgccgctgg catcaccctg ggcatggacg agctgtacaa gcctgcaggc
    11881 gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg gccgtccaat
    11941 ttactgaccg tacaccaaaa tttgcctgca ttaccggtcg atgcaacgag tgatgaggtt
    12001 cgcaagaacc tgatggacat gttcagggat cgccaggcgt tttctgagca tacctggaaa
    12061 atgcttctgt ccgtttgccg gtcgtgggcg gcatggtgca agttgaataa ccggaaatgg
    12121 tttcccgcag aacctgaaga tgttcgcgat tatcttctat atcttcaggc gcgcggtctg
    12181 gcagtaaaaa ctatccagca acatttgggc cagctaaaca tgcttcatcg tcggtctggg
    12241 ctgccacgac caagtgacag caatgctgtt tcactggtta tgcggcggat ccgaaaagaa
    12301 aacgttgatg ccggtgaacg tgcaaaacag gctctagcgt tcgaacgcac tgatttcgac
    12361 caggttcgtt cactcatgga aaatagcgac cgctgccagg atataaggaa tctggcattt
    12421 ctggggattg cttataacac cctgttacgt atagccgaaa ttgccaggat cagggttaaa
    12481 gatatctcac gtactgacgg tgggagaatg ttaatccata ttggcagaac gaaaacgctg
    12541 gttagcaccg caggtgtaga gaaggcactt agcctggggg taactaaact ggtcgagcga
    12601 tggatttccg tctctggtgt agctgatgat ccgaataact acctgttttg ccgggtcaga
    12661 aaaaatggtg ttgccgcgcc atctgccacc agccagctat caactcgcgc cctggaaggg
    12721 atttttgaag caactcatcg attgatttac ggcgctaagg atgactctgg tcagagatac
    12781 ctggcctggt ctggacacag tgcccgtgtc ggagccgcgc gagatatggc ccgcgctgga
    12841 gtttcaatac cggagatcat gcaagctggt ggctggacca atgtaaatat tgtcatgaac
    12901 tatatccgta acctggatag tgaaacaggg gcaatggtgc gcctgctgga agatggcgat
    12961 ctcgagccat ctgctggaga catgagagct gccaaccttt ggccaagccc gctcatgatc
    13021 aaacgctcta agaagaacag cctggccttg tccctgacgg ccgaccagat ggtcagtgcc
    13081 ttgttggatg ctgagccccc catactctat tccgagtatg atcctaccag acccttcagt
    13141 gaagcttcga tgatgggctt actgaccaac ctggcagaca gggagctggt tcacatgatc
    13201 aactgggcga agagggtgcc aggctttgtg gatttgaccc tccatgatca ggtccacctt
    13261 ctagaatgtg cctggctaga gatcctgatg attggtctcg tctggcgctc catggagcac
    13321 ccagtgaagc tactgtttgc tcctaacttg ctcttggaca ggaaccaggg aaaatgtgta
    13381 gagggcatgg tggagatctt cgacatgctg ctggctacat catctcggtt ccgcatgatg
    13441 aatctgcagg gagaggagtt tgtgtgcctc aaatctatta ttttgcttaa ttctggagtg
    13501 tacacatttc tgtccagcac cctgaagtct ctggaagaga aggaccatat ccaccgagtc
    13561 ctggacaaga tcacagacac tttgatccac ctgatggcca aggcaggcct gaccctgcag
    13621 cagcagcacc agcggctggc ccagctcctc ctcatcctct cccacatcag gcacatgagt
    13681 aacaaaggca tggagcatct gtacagcatg aagtgcaaga acgtggtgcc cctctatgac
    13741 ctgctgctgg aggcggcgga cgcccaccgc ctacatgcgc ccactagccg tggaggggca
    13801 tccgtggagg agacggacca aagccacttg gccactgcgg gctctacttc atcgcattcc
    13861 ttgcaaaagt attacatcac gggggaggca gagggtttcc ctgccacagc ttgatgaaga
    13921 tctgagctct taattaatga tcataatcag ccatatcaca tctgtagagg ttttacttgc
    13981 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
    14041 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
    14101 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
    14161 atcttatcat gtctggatcc gtttaaacta actttaaata attggcatta tttaaagtta
    14221 catatggcgg ccgccctaag gctcgacctc gaaattctac cgggtagggg aggcgctttt
    14281 cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag
    14341 tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt
    14401 tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc
    14461 gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca
    14521 gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca
    14581 gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct
    14641 caggggcggg ctcaggggcg gggcgggcgc ccgaaggtct tccggaggcc cggcattctg
    14701 cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc
    14761 gacctgcatc catctagatc tcgagcagct gaagcttacc atgaccgagt acaagcccac
    14821 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
    14881 cgccgactac cccgccacgc gccacaccgt cgatccagat cgccacatcg agcgggtcac
    14941 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
    15001 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
    15061 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
    15121 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
    15181 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
    15241 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
    15301 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
    15361 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc acgacccgca
    15421 gcgcccgacc gaaaggagcg cacgacccca tgcatcgatg atatcagatc cccgggatgc
    15481 agaaattgat gatctattaa acaataaaga tgtccactaa aatggaagtt tttcctgtca
    15541 tactttgtta agaagggtga gaacagagta cctacatttt gaatggaagg attggagcta
    15601 cgggggtggg ggtggggtgg gattagataa atgcntgctc tttactgaag gctctttact
    15661 attgctttat gataatgttt catagttgga tatcataatt taaacaagca aaaccaaatt
    15721 aagggccagc tcattcctcc cactcatgat ctatagatct atagatctct cgtgggatca
    15781 ttgtttttct cttgattccc actttgtggt tctaagtact gtggtttcca aatgtgtcag
    15841 tttcatagcc tgaagaacga gatcagcagc ctctgttcca catacacttc attctcagta
    15901 ttgttttgcc aagttctaat tccatcagaa gctggtcgag taactttaaa taattggcat
    15961 tatttaaagt taggcgcgcc ataacttcgt atagcataca ttatacgaag ttatgtcgag
    16021 atatttaaat attagaccca gctttcttgt acaaagtggt tgatatctct atagtcgcag
    16081 taggcggttc tctttcttgc tgtcccggaa aaaggatcct ctagagcaag ggtcattgct
    16141 ttctgctgtg tgggatgttc ttgagcatcc aggaaacatg tataaatgag cagggtaaca
    16201 tgttgattcc tcagatccaa tggttcccta gcccatttta atgtccaaat tactaataac
    16261 taacatgcct tggaaaggcg gggtgatgat aaaagggacc tataaaagcc tgttcctagt
    16321 ctcgtttgtg ccctcttccc gtctgtcccc tcccaaacag gagtcccctt caccttgtcc
    16381 agtgcttcag gagggcctgc gagtgtgagc ataggctctg tggacactcg agaaacatgc
    16441 tcagctcagc tcatcccctc tggctctttc tcccctgcca gccctgttcc caggatcaag
    16501 tggcgcaaag tggatggttc cttgtcccct cagtggggca cagcagagcc caccctgcag
    16561 atccccagtg ttagctttga agatgagggt acctatgaat gtgaggcaga gaactccaag
    16621 ggccgtgaca ccgtccaggg acgcatcatt gtgcaaggta ctgagctgga gatacctccg
    16681 tcttccctga ggaagcagca tgaaaatcca agagactggg aggccttctg cttgagcctt
    16741 ctgacccttg ccaggactgc taaacatagc tggagaggcc gtttcagagc tcagcggggg
    16801 catacagaaa gcacacaagt atttcaaaag atttagttgc taaatcagtc cctggcacaa
    16861 ccagagagtg ccagcttctt cgccctcatt tctttgttct ctccagccca gaataccaac
    16921 agcgccctct gctgtcactt acaaatactg cagccttata attccctatt cctctctttc
    16981 ttccccacag ctcagcctga gtggctaaag gtgatctcag acacagaagc cgacattggt
    17041 tccaacttac gttggggctg tgctgcggca ggcaagcctc ggcccatggt gcgctggctg
    17101 agaaacgggg aacctctggc ctcccaggta ggaaaccaag gcttcctccc acacgaccac
    17161 cctcttctgc tgcctggccc gcttaccctg ctgggccaac caaggggtct tgggacgttc
    17221 tggtgcttct tctcacagag agatacatgg ccacaggtgg gtcacctgtg tagcatcaca
    17281 gagatctggg atccctccat caaccagaaa tgtaacctta atctagcctc caggagacct
    17341 aaaggaccag gcagccctga agtagaccta tattcatgaa ccctcatgct gagggatgtt
    17401 agcttacagc ctctgcagca caccaccacc atcaccatca ccacccgccc ctgactcctt
    17461 cctggcctcc agtggtcctc atcttcctgc cttggctctt ctgcacagaa ccgggtggag
    17521 gtcttggctg gggacctgcg attctctaag ctgaacctgg aggactccgg catgtaccag
    17581 tgtgtggcag aaaacaagca tggcaccatc tatgccagtg ctgagctggc tgtacaaggt
    17641 aaaggaccca ggaggcaggg acagccctgg tgcttgaagg ttgtgtctgg ctgggcttct
    17701 ccaaatgcac acttgcaggc tcgtgtgcta acatacacat gtatgttctt tcttgttttc
    17761 caatggcccc tgagtagcac cagtttcaag acatgagatg gcgcaacgca attaatgata
    17821 acttcgtata gcatacatta tacgaagtta tggtctgagc tcgccatcag ttcatggcaa
    17881 gttacctgat tctatgcctc agtttcctga ttttaaaaaa tgcttcatcc tgtccttgaa
    17941 cagcgctttg aggggaattg aaattcaaag ggtgaactat gacaggcctg acattcagac
    18001 ggggacaagg aacaccagcc acttgagctt acaaggcacg ttttctctgc tgcaagatga
    18061 acgttagaag cctttattaa gtttaataag gctcaacaaa gacttactcg ttttattttt
    18121 tattttattt gtacgaatgc ctgtatttgt atgtgtatca catgttacag aagtcaaaag
    18181 aaagtgtaga ttctcctgaa actggagtta cagatggttg tgagccactg ggcactggaa
    18241 attaaactca ggtccatgct ctgacttact gagccatctc tccagcccca agactcttcc
    18301 tttcattcaa ttattcaacc aataacttac aaagcatctc atgaggagca aatacagtag
    18361 tacttaaata cttaaagtac tgtacaagaa aagggagagg aacaaactta gaatttattc
    18421 cagagaaccc taaaagtatg gttaaaaaaa taccaaggac agcagtggta cccaggtgag
    18481 caggttagat ggggtggtca ggaaagactc tgaggaagtc acgtttagac tgtgacctga
    18541 gggagacaga cttgtaaata tctggggaag tttcaggtaa tatgcatgca gacacaatga
    18601 tccaaaggca ggtaagactt ggcattcagg gggttaaaag gaaagctatg gggcgtggag
    18661 gtggaaagat gactcatcag ttaagaggac tggctgctct tacacagcag acccaggttc
    18721 catccccagt acccacaggg tggctcacaa ctacctctaa ttccagttcc agagactcat
    18781 acgccctctt ctggccgcct taggcactgc atgcaagtgg tacccagaca tacaagcagg
    18841 caaaacattc atatatatat atatgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtaa
    18901 agtaaaaata aaagaagtct ttaaaagaaa aagggaaaaa gttgaaagca agacagacag
    18961 ttggagcgtg tgagagggac gagtggagcc agatgtgatg acacaggggt agatgagttc
    19021 tagagatgtt gctgccactt gacaagttgg tctccagagg ccagatgctt agcacatagc
    19081 caggtgacat cttagttgag gtgggcgggt ggctatctcg agggtgagtg tttgagcacc
    19141 ctctctcctg cagctctggc cccagacttc aggcagaacc ctgtgaggcg gctgatccct
    19201 gcagcccgag ggggagagat cagtatcccg tgccagcccc gtgcggctcc aaaagctaca
    19261 atactgtgga gcaagggtac tgagattctg gggaacagta ccaggtaggt tctgtgtgcc
    19321 accgctgaga tgacgtcttg gaggtcagtc cccttccaat ggccacggtg tgtccctcag
    19381 ttgactagat ctttttctgt ccttgctgca gagtgactgt cactttggac ggcaccttga
    19441 ttatcagaaa catcagtcga tcggatgaag gcaaatatac ctgctttgct gagaacttca
    19501 tgggcaaagc caacagtacc gggatcctgt ctgtgcgagg taaggactgt ggtctgttca
    19561 gatgctggga tgctgttctg ccaaccatct gctcttggct tcctatacat ctgctttcaa
    19621 ggcaacgtca gaactcagtc tctcttggga ccacccctgg ccagcctcca gaatctgacc
    19681 actcatgaat atgcatctct tctaggggta caggccagag cctctctcct tcctgggctt
    19741 tttccctcac agagccttgt tttgttgttg ctgttttgct ttgtagatgc aacgaagatc
    19801 acgctagctc cttcgagtgc tgacatcaat gtgggtgata acctgaccct acagtgccac
    19861 gcctcgcatg accccactat ggacctcacg ttcacctgga ccctggatga cttccctgtt
    19921 gactttgata agcctggagg tcactaccgg agagccagtg tggtaaggcc tgaggcctca
    19981 taattctccc tccttccaga taagacaggg gacttgagat ggcctcagtc attaccagct
    20041 tcaggctttc caaagtttcc cctaaccccc gaggccatgc cgagaaagct caccctagga
    20101 cccaccgcca cctttcctgc atccacccaa ggaccaccct tctaagttag aatcaagctt
    20161 tagcattcca agtaccccag gagattctct ccactattgt ccctctcccc tagtgcaaat
    20221 gccatcatta acttggctca tgaagactgt cttatgtggc acgctttgat gtccagtggt
    20281 accaagtagc tcattcttcc cattgaacag ataggaacac tgaggcacag agagcatgga
    20341 tgccattcag ccagggaatc taggctccat ttcgtcttgt ggcctattac actagtcagg
    20401 gcttcctcct gtctcatcat tccttgctgt tccctgtgca cagaaggaga ccattgggga
    20461 cctgactatc ctgaatgccc agctacgcca cggagggaca tacacatgca tggcccagac
    20521 agtggtggat ggtgcatcca aggaggccac agtcctggtc cgaggtaatg ggagtcagcc
    20581 accacaggtc tcagagaggc tctaggcttt ccgactctcc ccacctctct tccctccctg
    20641 aaggctgaca gaagctggag agatggacac ttctgtttag tgcctgtgta gttctttgtg
    20701 ggtcctaaat gagtagacta tgtcttccaa agccatgatc aagaaagaaa atgtacccta
    20761 aacaccccta agactttacc tcatgagccc aggagttcca ggtccagtac agggcaaact
    20821 tgtacaccct tctgtgagcc accaagtctg agatgcttcg ctcctcatct ttctcctctg
    20881 cccccatcct ctggcctccc accctatctc tcctgccctc tgatgcccca tctagatact
    20941 gagtcagccc aagctgaggc tagataaaac cgactcaaac tgtccacctc tgacccaggg
    21001 tgctctgctt cttacaggtc ccccaggtcc tcctgggggt gtggtggtga gagacattgg
    21061 agacactact gttcagctta gctggagtcg tggctttgac aatcacagcc ccattgccaa
    21121 gtacaccctg caagcgcgga ctccaccctc agggaaatgg aagcaggttc ggaccagtaa
    21181 gtatgaggca ccatgtgggt cagttttaat aaccactgtg tgcacagcag aagacagggg
    21241 tcaaagggtc aaggctgacc ctaaggcttt gaaaagaatt aaggtgtgtt ctcttacctc
    21301 cagggagaac ctaggcaggc agggaaacct tcatagccag gagggtaagg agagggagag
    21361 ctggaggcag cagccttgtg ttgctagaga acgccggcat gctcaacatc cgtggaggga
    21421 agagcaaagg ccctgtggca tcagagctca ctagcttttc agacaagctt ttcagtcaaa
    21481 gagtaattga gggcttagcg aacgaacggt ag
//