LOCUS       232916#pL1L2_GT0_LF2A_nEGFPO_T2A_CreERT_puro#L3L4_pD223_DTA_T_spec        22579 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT0_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 232916
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000108180
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     misc_feature    4275..10026
                     /note="5 arm"
     misc_feature    10054..10078
                     /note="B1 Gateway"
     misc_feature    10091..10138
                     /note="Frt"
     misc_feature    10139..10638
                     /note="mouse En2 intron"
     primer          complement(10207..10228)
                     /note="LRPCR LAR3_EN2"
     primer          complement(10103..10126)
                     /note="FRT3"
     primer          14290..14308
                     /note="seq primer NF"
     misc_feature    14350..14357
                     /note="RE sites"
     misc_feature    16150..16183
                     /note="loxP"
     misc_feature    complement(14358..14389)
                     /note="Rox"
     misc_feature    complement(16110..16141)
                     /note="Rox"
     misc_feature    14390..14411
                     /note="polylinker"
     CDS             14970..15566
                     /note="puromycin N-acetyl transferase"
     misc_feature    15085..15090
                     /note="Mutated RsrII site"
     intron          10639..11013
                     /note="mouse En2 intron"
     misc_feature    11011..11013
                     /note="Splice Acceptor"
     exon            11014..11053
                     /note="mouse En2 exon"
     primer          10914..10935
                     /note="seq primer FCHK2"
     misc_feature    11054..11176
                     /note="1Dleader"
     misc_feature    11177..11228
                     /note="F2A"
     misc_feature    11324..12040
                     /note="EGFP"
     misc_feature    11234..11323
                     /note="3X NLS"
     misc_feature    14106..14344
                     /note="SV40 polyadenylation site"
     misc_feature    13139..14083
                     /note="ERT2"
     promoter        14422..14933
                     /note="PGK promoter"
     misc_feature    15677..16109
                     /note="PGK pA"
     primer          14164..14187
                     /note="LRPCR RAF5"
     misc_feature    12050..12103
                     /note="T2A"
     misc_feature    12100..12100
                     /note=" A to G to kill RsRII site"
     misc_feature    12104..13129
                     /note="Cre"
     misc_feature    11029..11054
                     /note="FCHK lf2a_lacz_gt0"
     misc_feature    11029..11044
                     /note="FCHK QC prefix region"
     misc_feature    11045..11048
                     /note="FCHK QC critical region"
     misc_feature    11049..11054
                     /note="FCHK QC suffix region"
     misc_feature    16206..16226
                     /note="B2 Gateway"
     misc_feature    10027..16256
                     /note="Synthetic Cassette"
     exon            16611..18200
                     /db_xref="ENSEMBL:ENSMUSE00000675858"
                     /type="targeted"
                     /rank="5"
                     /note="target exon 1 ENSMUSE00000675858"
     misc_feature    16257..18386
                     /note="Critical Region"
     misc_feature    18410..18443
                     /note="Downstream LoxP"
     misc_feature    18387..18466
                     /note="synthetic loxP region"
     misc_feature    18467..22579
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggaatgaac tgtttattta atagctatgg gttctttgac caccatcttc
     4321 caatctcttc tgccatctag ccatcccatc cgcattttct tttctgtgcc tatgaaatct
     4381 acgctagact gtacatgtga gtgaactact gcaatcgtgc ctctccgtgc ctggctgatt
     4441 tgacttgtac aacttcccat gtgaccatct atgtagttac aaatagcagg gtttgtgcgt
     4501 tttatgctct attgtattct actgtgtgca tatcctgtac ttcctctatc tattatagag
     4561 aattagctac tgagcattgt gctgcagcag taagtacagg gatacaaaca tactgtattt
     4621 atttcctttg gaaacacacc cagcagtggg gtttcatttt ttacttttta gagaattctc
     4681 aatggctcct tttctctgca gcctcaccaa cacatactaa gtttcaccct tctaataaca
     4741 gcaatcctga tgggtttttt ataatttttt gagacaggct ctcactatgt agccctggct
     4801 agcataggta gacttcctta tgtagaccaa gctggtttca aattcacata gatctatctg
     4861 cctgcctctg cttctggagt gctgaggtta agggtcatag ctgctctctt actatttttt
     4921 acatttggtc cttagtaaat gtagaacttg cagacatgtt cccacatctc tttgtactct
     4981 gtaattcctt tgtaattcct taatccctaa gtagaacctg tctttattat gatacatgcc
     5041 ccttggtcta gttttgcttt tgttccttca taccccaaat gaggcagtat atccagtatc
     5101 caaaaccatc atagtccaga ccaacacaat ggaattctgt gcacactccc atgcattctc
     5161 tgtagttcga gatcccatgt ttgagtcttt ctttcataca ctttgagtga atatttgtat
     5221 ctggtgagag acacagatct gttttcatgc tgtgaagtcg tacagttttc ccagtagcac
     5281 ttacagaagt gattttcttt ctctgctgtg tgtttttagc acctttgtca gatcagctgt
     5341 ctgtaaatgt atgggtttat ttctgggctc tctgttccat tctcctggtc tggctctgtg
     5401 tgatgccatg acagtgctgc ttatgggcta atgcagttta tgttatgttt gtaaaggggg
     5461 taagacaatg cttcttgctt tatttttctt gtagatttct ttggttcttc gggatctttt
     5521 gtggttccat actgttgata gaactctctc ccagggagag acataaagca ttgtgtagtc
     5581 ctcctctcag agtcctaatg accaacggaa acgtgaccca cctaaagctc cctctagaga
     5641 gccatgagtt tacagcttcc ttacagagta taagtgagag gtcacttaca gggtacggga
     5701 ctccttcccc aactagctgt acctgataac ccttacccag cagtggtgtg ggcttcctgt
     5761 agccctatct agtatttctt gactgataca taataagtat atatacgtat agtcagagat
     5821 atagagaata tagaatcttt ctacatatac tatatataca tactctttct atttccatat
     5881 atactatatc tctgaatgat acacacacac acacactaac ccctccccta acccacatgc
     5941 agagtcccca gatgggagga agcagttgga tgtagctgca tactccagca agaatcgcct
     6001 tgctatgctg gggcataaac caccaacaag cttagctgag atgaaatatt gcaggggaac
     6061 acagctgaat gccccaagat ggggcagttg cttgacatag aagacagtca ctcacaacac
     6121 attccaaaaa ataataataa taattaagag gcataaccag atttgaaaaa gccagctact
     6181 gccatggaag tcccttaaaa tatataaata tacatatatc aaaagtttaa atggagttac
     6241 cctatgttcc agttagtttc tattgctgtg attaaaacca tgaccaaaaa caacttgggg
     6301 aggaaaaatg tttaacctta caacttaaag ttcctcatga ggggaaatca ggacagaaac
     6361 taaagcagac catgttcaga gatttgttca gcaaccttct ccccaggggt agcaccgccc
     6421 acaaggaggc tgaaccctct cacatcaatc aagaacattc ttcacaaacc tgcctacagg
     6481 ctgaactgat gaggcatttc tttaatcaat gtcccctttt cctaggaaat tcaaccttat
     6541 gtcaagttga caaccacggc atcctataac agggaaatac tactcaaact agatagcaca
     6601 ggctagcaaa tacaaaccct aagagtaggc caccattttt gaggtgttac ccaaacaatg
     6661 atatctattg tcattcagaa cttggtggta ggactctagt gctaatgaca ccacctgctt
     6721 gagtcatgag catggaagtt tgatcaatac tgcctcgctt tcatagtgct aaaaggtgct
     6781 gtgtacacta atggaagaga agagttatca tcagtctcac ctaacagacc ccagagagct
     6841 tcaggaatga cctaagagct acagtaatgg cctgcctggc aactatgtcc actggtacaa
     6901 tagcggcaca gatgttatgg cgataactca ccaccttctg attgtattca agacccaccc
     6961 catgagatgg aactcatact tggcactgtt aatgaaagca agaacttcta gctaaaaatt
     7021 tcacggccct tagagaacaa cctactaata tcagtctaca tctaagcgga atgatattaa
     7081 aatgatgacc cccaataact taaggccatg cctgtagatc agtgcatctc ttcaactctc
     7141 atcagacagg tttctccttg caacagatgg cgattaacac agagacacac agctggtcaa
     7201 catacagaaa aattagagac ttcagagttc caagccctaa atgggacttc tgccactcct
     7261 tccagggatt aaggctcttc atggaacatg gggtgggaag actataagag ccccaagtga
     7321 tggatgactt caaggaggca atgttttcca gacacaagaa gacagtgcac atatgaactc
     7381 acagcattgc taacaacatg aacaaaacct gggcaagacc aaaccagaca aagtccaagc
     7441 atgggacgga gaggtgggca caaagtccta cccctctctg aggaactatt ggcatttgat
     7501 ggcttctcag aggaggagag tcaggcttct gtaatggtca ttaaacggtc aaccatactt
     7561 catagcagcc tcaaaacatc ggacattttt ggtggcagtg gaggtgatac gttttgtttt
     7621 taagagtggg gtggaggaga acaagatatt gactgggtgg gaagttagta ggggagggtc
     7681 tagaatagtg gtctctggct gtgggttaaa atgctttttg ggtcacatca tacatccttc
     7741 atatcagata tttacattat atttcataaa aatagtaaaa ttatagctat gaagtagcaa
     7801 tgaaataatt ttatggacgg agtcagcaca ccatgaggga ctgaatgaaa aggtcgcagc
     7861 atcaggaagg ttgagaatca ctgctctagg aggagttggt ggaggaggtg aatatcatca
     7921 aaatagattt gtgaaattct cagagaatga ataaaaatat tgtttttgta aaaagaggca
     7981 gaaccaggcc atgtgtggtg atgtacagat ataattctag aacttggaag gcagaggaag
     8041 tcagagttta aagacagcct ggatatatag tgaggagaga ggggggggag agggagaggg
     8101 ggagagagag aggggagaga gagagagaga gagagaggga gagagagaga gaagagagag
     8161 agaagagaga gagagggggg gagggagaaa gagaggagag agagggagag agagagaggg
     8221 agagagagag ggagagagag ggagagagag ggagagagag agaaagaaga atgttgccca
     8281 ggatgacctc tatctaattt cagacaaccc tctcagcttt tcaggtagcg agaactataa
     8341 ggcatatgtc accaaaactg taaggtattt ttgacttcta taaataatat cattcatact
     8401 tcagtactgg ttgaatcaga ttgtatatag atttgcctag acaatatgaa tgtattaaca
     8461 atattaattc ttgtagccca cgtatacaga gtatccgtct gtttgtctct atgtctccag
     8521 cttctttctt caatgtttta ttggttttcc ctgtagagat ctttaacctc gttggttaga
     8581 tttgttcctc catgtttcat gtagctctcg aaaatgggat ttccacttca gacaactcac
     8641 tgctagtata taaagtgctg cttggtttcg tatgttgatt tttgtgttta ataagtttat
     8701 tcttagttca tgatcataaa cactggactc ttcagggtta cttgttctta aaaatcatgt
     8761 catctgaaag ttgtaacaat ttaacttctc cctttctggc cttttattta gttcctcctt
     8821 ccatacagga tttctctgtc tttattccaa atgggcttgt tactgatggc tgaatccctc
     8881 tgaactaaca gattgtttat tttatttatt tatttaccta acaggttatt tatatttggt
     8941 ttttatcaag tacttccaca acatcgacat ttaaaatccc attcggtgga gactccagga
     9001 tctttgctct ctctggctcc aagagttttg agagcacagt gggttgaatc ttgtgcagtg
     9061 gagacaggtc tcttaatgag cttgggctgg ctccctgtgc tgcaggaagt ttaagacatg
     9121 cagaagtcag ccacccagct taccgctcca tcttcctccc actccctccc ccgcagaaac
     9181 ttttgatcac tgccactgta gctgcagcaa gcacccaaga cagctgctct tgtttgatgt
     9241 gttcacatga tttgtagatc tgtctccctt cccccggggt cagcagcggg cttcataaga
     9301 atctacggga aatcaaatag tatatgcagg gttaggaagc ctgccctgag acccacagtt
     9361 tagacatttc tatcttttca acaacaacaa caaaggcagc tagacacagg caacatactt
     9421 ttaattgtct aagtcattgt ctttgtgtgt gtctgtgtac acacatatgt ggaagctatg
     9481 catgagtaca tttttcagcc tgtaaaatat actggagaag cagcaagaca tagactgagc
     9541 tgcagaaggt tttagacatc tccatggcaa agctgctgat gataattagg gaaggaatgc
     9601 gtccgtgccc tttactactt ggaggctaca gttattgaag tgacagctga ctgtgtagat
     9661 ctgtacactc tcaccctact ctctttgaac caacagttag tttcttgtaa gcaatcatca
     9721 tcaactttgt cattataaga tcagctatga cacgctaatt aaaaaatccc aagtagggcc
     9781 aggcacagtg gtgcgcatct ttaatcccag cccttgggag agagaagcag gcagatctct
     9841 gttgcatttg agggcagtct agtctacata gtgagtttca ggacagccag gtctacatag
     9901 tgagaccctg tctcaaaaaa aaaaaaaaga aaagaaaaaa gaaaaaaaaa tcccaagtag
     9961 aagctgtgaa ggttgagctg agcaaaagag ggattttcag tgtttctttt aacattgtaa
    10021 tgaaaaaagg cgcataacga taccacgata tcaacaagtt tgtacaaaaa agcaggctgg
    10081 cgccggaacc gaagttccta ttccgaagtt cctattctct agaaagtata ggaacttcga
    10141 accctttccc acaccaccct ccacacttgc cccaaacact gccaactatg taggaggaag
    10201 gggttgggac taacagaaga acccgttgtg gggaagctgt tgggagggtc actttatgtt
    10261 cttgcccaag gtcagttggg tggcctgctt ctgatgaggt ggtcccaagg tctggggtag
    10321 aaggtgagag ggacaggcca ccaaggtcag cccccccccc ctatcccata ggagccaggt
    10381 ccctctcctg gacaggaaga ctgaagggga gatgccagag actcagtgaa gcctggggta
    10441 ccctattgga gtccttcaag gaaacaaact tggcctcacc aggcctcagc cttggctcct
    10501 cctgggaact ctactgccct tgggatcccc ttgtagttgt gggttacata ggaaggggga
    10561 cgggattccc cttgactggc tagcctactc ttttcttcag tcttctccat ctcctctcac
    10621 ctgtctctcg accctttccc taggatagac ttggaaaaag ataaggggag aaaacaaatg
    10681 caaacgaggc cagaaagatt ttggctgggc attccttccg ctagctttta ttgggatccc
    10741 ctagtttgtg ataggccttt tagctacatc tgccaatcca tctcattttc acacacacac
    10801 acaccacttt ccttctggtc agtgggcaca tgtccagcct caagtttata tcaccacccc
    10861 caatgcccaa cacttgtatg gccttgggcg ggtcatcccc ccccccaccc ccagtatctg
    10921 caacctcaag ctagcttggg tgcgttggtt gtggataagt agctagactc cagcaaccag
    10981 taacctctgc cctttctcct ccatgacaac caggctccca ggtcccgaaa accaaagaag
    11041 aagaacgcag atctctagag tcaccgagtt gctttaccgg atgaagaggg ccgaaacata
    11101 ctgtccaagg cccttgctgg caatccaccc aactgaagcc agacacaaac agaaaattgt
    11161 ggcaccggtg aaacagactt tgaattttga ccttctcaag ttggcgggag acgtcgagtc
    11221 caaccccgga ccggtgcatg tggaccccaa gaaaaagcgg aaggtggacc ctaaaaagaa
    11281 acgcaaagtg gatcctaaga aaaaaagaaa ggtgcacgtg tccatggtgt ccaagggcga
    11341 ggaactgttc accggcgtgg tgcccatcct ggtggaactg gatggcgacg tgaacggcca
    11401 caagttcagc gtgtccggcg agggcgaagg cgacgccaca tacggaaagc tgaccctgaa
    11461 gttcatctgc accaccggca agctgcccgt gccttggcct accctcgtga ccacactgac
    11521 ctacggcgtg cagtgcttca gcagataccc cgaccatatg aagcagcacg acttcttcaa
    11581 gagcgccatg cccgagggct acgtgcagga aagaaccatc ttctttaagg acgacggcaa
    11641 ctacaagacc agggccgaag tgaagttcga gggcgacacc ctcgtgaaca gaatcgagct
    11701 gaagggcatc gacttcaaag aggacggcaa catcctgggc cacaagctgg agtacaacta
    11761 caacagccac aacgtgtaca tcatggccga caagcagaaa aacggcatca aagtgaactt
    11821 caagatccgg cacaacatcg aggacggctc cgtgcagctg gccgaccact accagcagaa
    11881 cacccctatc ggcgacggcc ctgtgctgct gcctgacaac cactacctga gcacccagag
    11941 cgccctgagc aaggacccca acgagaagag ggaccacatg gtgctgctgg aattcgtgac
    12001 cgccgctggc atcaccctgg gcatggacga gctgtacaag cctgcaggcg agggcagagg
    12061 aagtcttcta acatgcggtg acgtggagga gaatcccggg ccgtccaatt tactgaccgt
    12121 acaccaaaat ttgcctgcat taccggtcga tgcaacgagt gatgaggttc gcaagaacct
    12181 gatggacatg ttcagggatc gccaggcgtt ttctgagcat acctggaaaa tgcttctgtc
    12241 cgtttgccgg tcgtgggcgg catggtgcaa gttgaataac cggaaatggt ttcccgcaga
    12301 acctgaagat gttcgcgatt atcttctata tcttcaggcg cgcggtctgg cagtaaaaac
    12361 tatccagcaa catttgggcc agctaaacat gcttcatcgt cggtctgggc tgccacgacc
    12421 aagtgacagc aatgctgttt cactggttat gcggcggatc cgaaaagaaa acgttgatgc
    12481 cggtgaacgt gcaaaacagg ctctagcgtt cgaacgcact gatttcgacc aggttcgttc
    12541 actcatggaa aatagcgacc gctgccagga tataaggaat ctggcatttc tggggattgc
    12601 ttataacacc ctgttacgta tagccgaaat tgccaggatc agggttaaag atatctcacg
    12661 tactgacggt gggagaatgt taatccatat tggcagaacg aaaacgctgg ttagcaccgc
    12721 aggtgtagag aaggcactta gcctgggggt aactaaactg gtcgagcgat ggatttccgt
    12781 ctctggtgta gctgatgatc cgaataacta cctgttttgc cgggtcagaa aaaatggtgt
    12841 tgccgcgcca tctgccacca gccagctatc aactcgcgcc ctggaaggga tttttgaagc
    12901 aactcatcga ttgatttacg gcgctaagga tgactctggt cagagatacc tggcctggtc
    12961 tggacacagt gcccgtgtcg gagccgcgcg agatatggcc cgcgctggag tttcaatacc
    13021 ggagatcatg caagctggtg gctggaccaa tgtaaatatt gtcatgaact atatccgtaa
    13081 cctggatagt gaaacagggg caatggtgcg cctgctggaa gatggcgatc tcgagccatc
    13141 tgctggagac atgagagctg ccaacctttg gccaagcccg ctcatgatca aacgctctaa
    13201 gaagaacagc ctggccttgt ccctgacggc cgaccagatg gtcagtgcct tgttggatgc
    13261 tgagcccccc atactctatt ccgagtatga tcctaccaga cccttcagtg aagcttcgat
    13321 gatgggctta ctgaccaacc tggcagacag ggagctggtt cacatgatca actgggcgaa
    13381 gagggtgcca ggctttgtgg atttgaccct ccatgatcag gtccaccttc tagaatgtgc
    13441 ctggctagag atcctgatga ttggtctcgt ctggcgctcc atggagcacc cagtgaagct
    13501 actgtttgct cctaacttgc tcttggacag gaaccaggga aaatgtgtag agggcatggt
    13561 ggagatcttc gacatgctgc tggctacatc atctcggttc cgcatgatga atctgcaggg
    13621 agaggagttt gtgtgcctca aatctattat tttgcttaat tctggagtgt acacatttct
    13681 gtccagcacc ctgaagtctc tggaagagaa ggaccatatc caccgagtcc tggacaagat
    13741 cacagacact ttgatccacc tgatggccaa ggcaggcctg accctgcagc agcagcacca
    13801 gcggctggcc cagctcctcc tcatcctctc ccacatcagg cacatgagta acaaaggcat
    13861 ggagcatctg tacagcatga agtgcaagaa cgtggtgccc ctctatgacc tgctgctgga
    13921 ggcggcggac gcccaccgcc tacatgcgcc cactagccgt ggaggggcat ccgtggagga
    13981 gacggaccaa agccacttgg ccactgcggg ctctacttca tcgcattcct tgcaaaagta
    14041 ttacatcacg ggggaggcag agggtttccc tgccacagct tgatgaagat ctgagctctt
    14101 aattaatgat cataatcagc catatcacat ctgtagaggt tttacttgct ttaaaaaacc
    14161 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
    14221 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
    14281 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
    14341 tctggatccg tttaaactaa ctttaaataa ttggcattat ttaaagttac atatggcggc
    14401 cgccctaagg ctcgacctcg aaattctacc gggtagggga ggcgcttttc ccaaggcagt
    14461 ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc
    14521 ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct
    14581 tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt
    14641 cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca
    14701 ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct
    14761 tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc
    14821 tcaggggcgg ggcgggcgcc cgaaggtctt ccggaggccc ggcattctgc acgcttcaaa
    14881 agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcatcc
    14941 atctagatct cgagcagctg aagcttacca tgaccgagta caagcccacg gtgcgcctcg
    15001 ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc
    15061 ccgccacgcg ccacaccgtc gatccagatc gccacatcga gcgggtcacc gagctgcaag
    15121 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
    15181 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
    15241 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
    15301 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct
    15361 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
    15421 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
    15481 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
    15541 ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg
    15601 aaaggagcgc acgaccccat gcatcgatga tatcagatcc ccgggatgca gaaattgatg
    15661 atctattaaa caataaagat gtccactaaa atggaagttt ttcctgtcat actttgttaa
    15721 gaagggtgag aacagagtac ctacattttg aatggaagga ttggagctac gggggtgggg
    15781 gtggggtggg attagataaa tgcntgctct ttactgaagg ctctttacta ttgctttatg
    15841 ataatgtttc atagttggat atcataattt aaacaagcaa aaccaaatta agggccagct
    15901 cattcctccc actcatgatc tatagatcta tagatctctc gtgggatcat tgtttttctc
    15961 ttgattccca ctttgtggtt ctaagtactg tggtttccaa atgtgtcagt ttcatagcct
    16021 gaagaacgag atcagcagcc tctgttccac atacacttca ttctcagtat tgttttgcca
    16081 agttctaatt ccatcagaag ctggtcgagt aactttaaat aattggcatt atttaaagtt
    16141 aggcgcgcca taacttcgta tagcatacat tatacgaagt tatgtcgaga tatttaaata
    16201 ttagacccag ctttcttgta caaagtggtt gatatctcta tagtcgcagt aggcggtggc
    16261 tctcaacagg actgacaggg acagacatct ataggcttcc cattgttctc cgcatttttg
    16321 ccaggatatt tcattagtgt gaacagtgaa ccctttgtca tcttgcctca ccacttgacc
    16381 ttggcgattc tcaattctgc tggtgccgtg aaggacggag gtggcaggca cgagacaacc
    16441 actaattatt ggaagcagaa gccttgctga cccagtatga gattaactgc tcctttgcag
    16501 tttttatttt agggcattag caactgtctg gttatcttgc tttggttgag aaattcagga
    16561 caatagcagt gctgatggta acgttggcga tttccctgtt tgttttgcag gtcacggcca
    16621 ggggctttgg gccactgcta cagtttgcct acactgccaa gctgttactc agcagagaga
    16681 acatccggga ggtcatccgc tgtgctgagt tcctgcgcat gcacaatctg gaggactcct
    16741 gcttcagctt cctgcagacc cagctcctga acagggagga tggcctgttt gtttgcagaa
    16801 aggacagtgc gtgccagcgc ccccaagagg accatgggaa ttctgcagga gaagaggagg
    16861 aagaagagga aacaatggac tcagagacag ctaggatggc ttgcgccaca gaccagatgc
    16921 tcccagaccc catcagcttt gaggccactg ccatcccagt agcagagaaa gaagaagccc
    16981 tgctccctga gtctgaggta cccacagaca ccaaggaaaa ctcagaaaag ggtgcattga
    17041 cccagtaccc tagatataag aagtaccagc tcgcgtgtac caagaatgtc tacagtgcac
    17101 cctcacacgg tacctcaggg tttgcgagca cattcagtga agacagtcct ggcaatagcc
    17161 tcaagccggg gctccccatg ggacagatta aaagtgagcc acccagtgag gagaccgagg
    17221 aggagagcat caccctctgc ctgtccggag atgagaccga catcaaagac agaccaggag
    17281 atgtcgagat ggaccgaaaa caacccagcc ctgcccctac ccccagtacc cctactggag
    17341 ccgcctgcct ggacaggtcc aggagtgtgt cctcgccttc ctgcctgcgg tctctgttcg
    17401 gtataacgaa aggtgtggag tccactggcc tgcccagtac atctcagcaa cccttagtca
    17461 ggagctcagc gtgccctttc aacaagggga tctctcaggg tgaccttaaa actgattaca
    17521 cccctttggc agggaattac ggacagcccc acgtgggcca gaaggatgtg tccaacttcg
    17581 caatggggtc gccactcagg gggcccgggc cggagactct ctgtaagcag gagggagagc
    17641 tggaccgccg gagcgtcatc ttctccgcaa gcgcgtgtga ccaaccgaac actccagtgc
    17701 attcatactc tgcggtgagc aatttggaca aagacctttc ggagccggtg ccaaaaagtc
    17761 tatgggtggg agctggccag tccctcccca gctcccaggc ttactcccac agcggactga
    17821 tggctgacca cttgccagga aggatacggc ccaacaccag ctgcccggtg ccaatcaaag
    17881 tctgtcctcg ctcacctccg ctcgagacca ggactaggac ctccagctcc tgctcctcgt
    17941 attcctacgc agaggacggg agtgggggct ccccctgcag cctccctctc tgtgagttct
    18001 cctcttcgcc ctgttcccag ggagccagat tccttgccac ggaacatcag gaaccaggcc
    18061 tgatgggaga tggaatgtac aaccaagtcc gaccccagat taaatgtgag cagtcttacg
    18121 gaaccaattc cagtgacgag tctggatcat tctcggaagc agacagtgag tcgtgtcctg
    18181 tgcaggacag gggccaggag gtagggaatc ctaagtttca agcgtgtgac ctactatctc
    18241 agtcctttcc ctctacccaa acatcactct caggtcgttc ttttatgttg tcatagttag
    18301 ggtttctgtt gctgtgacta aacgccatga ccaaaaacca atctgaggag gaaagagttt
    18361 atctggttta cgcgtgcatg tttctggaga tggcgcaacg caattaatga taacttcgta
    18421 tagcatacat tatacgaagt tatggtctga gctcgccatc agttcaagcc cgcattcttt
    18481 tagaactaag gagcaccagc tcagggatgg caccacccac aatgggatgg accctccccc
    18541 accaatccct aatttaagaa attaccctcc agccagatct catggaggca ttttctcaat
    18601 taagattccc tccttccaga tagctctagt ttatgtgtcc agctgacatg agatcagcca
    18661 gcacaggtgt caaggttaaa ggtgaccata aatggtgtgg taactgtgaa tggcctgtct
    18721 cttccttggc ggcaggatgc tgcagtgtga gtcagaatca accatgcagc cttagaacga
    18781 actccctttg catctaacct ggattcctgt gctcagtgag cagaggctgg ctgaatgcag
    18841 tgcatgtaag gtccatatac ttttggtctc cttccctttt cctctgctgc catcataccc
    18901 ctctcttcca cctactttgg aagagtgccc tcgagtttgc tctgtatccc ttcggttcaa
    18961 agggaaatga agaagctcat tcaaattgac tgattgggac tgagtagggc ctgacaaaca
    19021 ggtgcctctc cctggccttc taagtatttc ttgtcaatag aagactccgg ggtgaccttg
    19081 tgtctgtcac agtgcatgac agaatgctca tcatccgtct tagacatggg atccattcag
    19141 aaatgtcttg ttggcacaca taactgtgtc agggcttgtt tctttcccag ggtccacaaa
    19201 tgagaagaag tcctgtatgt ggtggtgagt ggttttatgc agcctgaaag tgctgtgctt
    19261 tgtagagaaa aagcttataa ggcttcatct gcgggtcact gtttctggga gtggaaatgg
    19321 ggacagcctg acgacagcag aagcaagcct cacatgacta tgagaaagag gaatgggact
    19381 cccagaggtc cctagttcgc tggtaatttg aatgttccct agacaaacct aagcatgtct
    19441 aggtcatgac ctggtaccca cggcagtgtc ttactgattg acgaagaacc acctaaagac
    19501 cgagactatg tctcacaccc gtctcaggag cagaagtgag caaacccagt ggcagatctt
    19561 gcccctgtgt ctgatgaaca caggaaagag aagcacaggt tgggaatatg actccatgat
    19621 atggggctaa cctaccagga cctggccctg gaaatccatt taaaaaacga aatgagggga
    19681 gggaagagag ggaggacaga aagggaggtg gctgcccagg aggccagcac gctcatggtt
    19741 ggtctgctct tcagttatca gcaaagatga actctaccag tcggtagaag tgtctcgagg
    19801 ggtgaggaga ggtagccaca gagcaggtgt attctgcatc cacaagctgg tgggcagaac
    19861 tcgcaagagc tttaacttca gacactgaaa atggactcca gagctgcctc gagctagctg
    19921 cggacacacc ctgggttcac ttcctcttct ttgctagtct gcctggtccc tgctttcctg
    19981 ttactgtctg gcagaaatag agagacagaa aaccccctga aaagggttta atttaagaaa
    20041 gagaaattgt gaaggaagtc cggatgggat ctgttcctgt ttgaatctta ccccttcact
    20101 gcgagattga gttgctatca atgcccaaga cacggcagcc ctggtccaac ctttaggagc
    20161 aaggcttcct aaaaccaagg ataagtgtgt gttctctctg ttcttccttc ccacagcctt
    20221 cctgaggttt gccttttgtc cctaactgtc cttaagtgtg cctaatgcag aaaactagtc
    20281 attgagtcat gaccatccca caaatgatcg tacttaccaa ggactggcag agcacgtgca
    20341 gatgcctccg tgtcttcctt gggtctcgaa aagagcctta cacttagccc aagaaattac
    20401 cttccttagg aactgtcagt ttaccaacgc catccacatg catgtctgtt gttcttgttg
    20461 ttgtttattt ttgttttgtt ttgttttttc tgattgggct ctgtcaatct ccaccttaga
    20521 actgaaggct atgttacagg gtgaggagtc attcagcctg tgaaacagcc acaaacagga
    20581 ttaatctctc cttaaaatac ccttgcattt cttttttttt tggggggggg gggggggaag
    20641 tggttttttt ttgagacagg gtttctctgt attgccctgg ctgtcctgga actcactttg
    20701 tagaccaggc tggcctcgaa ctcagaaatc tgcctgcctc tgcctcccga gtgctgggat
    20761 taaaggcatg caccaccatg cccagctttg catttcattt ttatctttac ttttcaaaac
    20821 aaacctgttg tttattcata agcagtaaaa gacacatttg ccgaaactca ctctgcccct
    20881 cttgttagct tgtgttagac aagaggatga taacaaagct gagggtgtgt gtgtgtgtgt
    20941 gtgtgtgtgt gtgtgtgtgt gtgtgtggtt ccgctccccc aacctcaccc cactccccag
    21001 aaaaggaatt cactttccca gaagcaccca caccctcagc acacggcccc tctgggagtc
    21061 agttacttgt ttcaagagtt acttggtctg ggtagacagt ctgcctcaaa cgtacactga
    21121 aattaacttg attacttgaa ctgatccatt ttaaaacacc agcatctatc tgggattcta
    21181 caaatactgc tcttaggtgg ggtttaacca agtaaaccgg gaaagacaac cgagtctacc
    21241 tcagcccggg gccctctctg aagcagctac cagcaagtac agcagtttct ctgcagcttc
    21301 tgtagggagc caaaatgttg gaccagaaga aaaacgttag catatctttc cttctgtaaa
    21361 atctatcact tgttaaatga ctctcctagt tcataaaaca tagacactcc acccccccat
    21421 cctttttcac ccttccctag ctttgaaaaa gcttaaatta tcgggttggc gagctggctc
    21481 agcagttaca agtatgggct gctgtttcag aggacccagg ttcagttccc agcgcccaca
    21541 ctcatgggtg gctcacagtc ttctgtaacc ctggcctcgg caaaacactg cacatacatg
    21601 gtacaccgac cagacataca aataggcaga acaactatac acataaaaaa taaatagcat
    21661 agtgcacgcc tttaatccca gcacttggga ggcagaggca ggtggatttc tgagttcaag
    21721 accagcctgg tctacaaagt gagttccagg acagccaggg ctatacagag aaaccctttc
    21781 tcaggagaag aaagagagag agagagagag agagagagag agagaataaa tagatagata
    21841 gatacataga tagatagata gatagatagc aaatttttaa atgaaatata atttccatgc
    21901 cataccataa aatttcccct attagaatga aagtttcagt gtttttagta ttttcacagt
    21961 gttaggcaac tgtcttccgt ttttggctct agcgtatttt ctttgaccta ggaagagtcc
    22021 tgtccccagt agcaggttct ccccacggct tctttctgtc caccccaggc aaccagtaac
    22081 cacctctgtc ctgtactaga tgttttgtat aaatggtatt tggttttttt tcaaaaaggt
    22141 atcattttca aaatgtatcc cttccatagt tacacacacc catcatatgt agctccacat
    22201 gctgattgct gatacattgt gtctaggcct gccacatttt attgatccgt cattgcttga
    22261 gggagtttgg cgggtcctac cttctgaatg tttctgaaga atggtgctat gagcatttgc
    22321 atgcaagggt ttttaggacg ggcttccagt tcattcactc agtacctctg agtggaactg
    22381 ctgtgtcacg tggggactcc atgtggcctt tgagggacta ttcagccatt tcccaaggca
    22441 gcccttccaa ctttgcattc cccctatcag gctagttctt cttctcactg ctaatactta
    22501 actcttctcc gtgtgtctat ctgatggatg tgacatggca tctccttagg tttacaagtt
    22561 tgtttttaga ttttgaaaa
//