LOCUS       211858#pL1L2_GT0_LF2A_nEGFPO_T2A_CreERT_puro#L3L4_pD223_DTA_T_spec        22151 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT0_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 211858
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000021610
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            7076..7277
                     /db_xref="ENSEMBL:ENSMUSE00000384183"
                     /rank="1"
                     /note="ENSMUSE00000384183"
     exon            7992..8038
                     /db_xref="ENSEMBL:ENSMUSE00000115495"
                     /rank="2"
                     /note="ENSMUSE00000115495"
     misc_feature    4275..10262
                     /note="5 arm"
     misc_feature    10290..10314
                     /note="B1 Gateway"
     misc_feature    10327..10374
                     /note="Frt"
     misc_feature    10375..10874
                     /note="mouse En2 intron"
     primer          complement(10443..10464)
                     /note="LRPCR LAR3_EN2"
     primer          complement(10339..10362)
                     /note="FRT3"
     primer          14526..14544
                     /note="seq primer NF"
     misc_feature    14586..14593
                     /note="RE sites"
     misc_feature    16386..16419
                     /note="loxP"
     misc_feature    complement(14594..14625)
                     /note="Rox"
     misc_feature    complement(16346..16377)
                     /note="Rox"
     misc_feature    14626..14647
                     /note="polylinker"
     CDS             15206..15802
                     /note="puromycin N-acetyl transferase"
     misc_feature    15321..15326
                     /note="Mutated RsrII site"
     intron          10875..11249
                     /note="mouse En2 intron"
     misc_feature    11247..11249
                     /note="Splice Acceptor"
     exon            11250..11289
                     /note="mouse En2 exon"
     primer          11150..11171
                     /note="seq primer FCHK2"
     misc_feature    11290..11412
                     /note="1Dleader"
     misc_feature    11413..11464
                     /note="F2A"
     misc_feature    11560..12276
                     /note="EGFP"
     misc_feature    11470..11559
                     /note="3X NLS"
     misc_feature    14342..14580
                     /note="SV40 polyadenylation site"
     misc_feature    13375..14319
                     /note="ERT2"
     promoter        14658..15169
                     /note="PGK promoter"
     misc_feature    15913..16345
                     /note="PGK pA"
     primer          14400..14423
                     /note="LRPCR RAF5"
     misc_feature    12286..12339
                     /note="T2A"
     misc_feature    12336..12336
                     /note=" A to G to kill RsRII site"
     misc_feature    12340..13365
                     /note="Cre"
     misc_feature    11265..11290
                     /note="FCHK lf2a_lacz_gt0"
     misc_feature    11265..11280
                     /note="FCHK QC prefix region"
     misc_feature    11281..11284
                     /note="FCHK QC critical region"
     misc_feature    11285..11290
                     /note="FCHK QC suffix region"
     misc_feature    16442..16462
                     /note="B2 Gateway"
     misc_feature    10263..16492
                     /note="Synthetic Cassette"
     exon            16803..16896
                     /db_xref="ENSEMBL:ENSMUSE00000260058"
                     /type="targeted"
                     /rank="3"
                     /note="target exon 1 ENSMUSE00000260058"
     exon            17626..17694
                     /db_xref="ENSEMBL:ENSMUSE00000260051"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 2 ENSMUSE00000260051"
     misc_feature    16493..17798
                     /note="Critical Region"
     misc_feature    17822..17855
                     /note="Downstream LoxP"
     misc_feature    17799..17878
                     /note="synthetic loxP region"
     exon            19762..19920
                     /db_xref="ENSEMBL:ENSMUSE00000260045"
                     /rank="5"
                     /note="ENSMUSE00000260045"
     exon            20084..20500
                     /db_xref="ENSEMBL:ENSMUSE00000491741"
                     /rank="6"
                     /note="ENSMUSE00000491741"
     exon            21015..21490
                     /db_xref="ENSEMBL:ENSMUSE00000115500"
                     /rank="7"
                     /note="ENSMUSE00000115500"
     misc_feature    17879..22151
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggacagagc gcacaatagg tgctcaataa gtgtttgatg actaatgaaa
     4321 taatttggct cggttccaaa gtattctagt tgaactaatg aacaggagtt catgcgcata
     4381 acaagcccct gctgggtcac tgagcctctg ggaggggatt ggcaacaaca tttaggaagg
     4441 agaagcatcc taagacattt ccccaaacaa tcaccaagga acagtcaagc cacagagcca
     4501 acgtggaggg ggaaacacct gcatcctgga gaaagaccca gactctgtgc agttaccaag
     4561 gaccaaagac catcagagga gcagggtact gtcagcccac ccacacccct gaatcaaatg
     4621 gtgttgacca gcatttgagc atttgaactt gggttccctg agataatgat tctgtttacc
     4681 agagacccct atgagagaga gggggggggg aatgggagag ggaggggagt agacaaagga
     4741 ttgatggagt gtgcactatt ggcagtgtgc tattgataaa gtaagatatc agcgaagtgt
     4801 ctgttattaa tataaaactc tactgataaa gtacattact ggtggtgtcc tgtcaatagt
     4861 gtctgccatt gataaatcac actattgaaa aggtgccgtc acttaaatat atgctattga
     4921 taaaagtgtg ctgttgataa agtatgtact cttggagcag atggcaaagt gagaatcggg
     4981 gtttaattct tacgggtgat agtgatgtta ctggaagtcc tcttcaatgc atcccaccaa
     5041 ggggaggaag ccccttgtga actctgaaac tagaggtggc ctttggctcc cagtaaatct
     5101 ctgttacaca ctatgctaaa ataagtacat atttatattt ttacataata tgaaaataaa
     5161 tataaaaata aaacagccag cacatctaaa ctgtgaccca cataagggtg tctgggatga
     5221 tgatgcccag agtgcaagga gggtttattt gtttatttat ttatttagaa acagggtctc
     5281 actatgtagc tctggctcgt cctggaactc gtcacatgga ccaggctggc attgaactca
     5341 gagatctgct tgattctgac ccccgggttc agggattaga cgtgtgtgcc accatgcctt
     5401 gctgaggcta ctatttacga gacgatgcta ggagcttgca catgatcaca gcgacctccc
     5461 atcctaggac ctggaacaac cgttctccca gtgctgagct ggagtccttc tctaggaact
     5521 aatattaaat gataggagat gccttaaata aatgccttaa aagaaagttg tcggcccttg
     5581 tccctgggtg gagccactgt gagggatctc ctatatttct caagattgag atgagatgtg
     5641 tgctattcat ctctgaaagc cttgtatgac ccacctgctt gctgttcctt agaatggacc
     5701 agacaccttc tcccttaggg ggcgtttggc tggctgctcc ctcccctgga gagagagctc
     5761 cccctctaca caggttctgg ctcactttaa atgccacctc ctggtaaggc ctcctcagaa
     5821 ccagcctctt caatgtattc ctgtccctca ctagaactcc gcccgcttct ctgctttatg
     5881 ctcgtagcat ggccactacc cagcacagtg tacatcttgt ttctttctct ggaaagggaa
     5941 ttctataagg gttgggtttg ctgttttgtt tacagctgcg tctttggcac ccagcacagc
     6001 tgagtggttc taagcccacg tcgatgctta acacatggtt gttgaatgaa tacacgcgaa
     6061 gccggttctc atttaggggc atgagtaggc agaggtgtgg gcaggaagca ggaaagagcg
     6121 gaaacaggtg cggacagaaa ggaggggctc tgaaggatgc cagtcagtgc caaactgtca
     6181 tccagatacc aggttcactg tggccctagg cccaggctgc acggggcttc ccatgtggtc
     6241 tgcccagggt gagagcagaa ctgcggtggg cggggcagaa ggaaaccaac caggaagcag
     6301 ggttgcaccc aaattatcca ggttttaagt acatttaaga gacaaggctg ggctgttgaa
     6361 ggcagaggtg tccctggggt gctggactag gactgaccac ttctgtttta gtttaatggt
     6421 gagaactgcc tcacactgct acctgcctta cttgcccctt gagagctgtg agcctaggac
     6481 ccacccatgt gtgggttgga ccttcagtcc acacactgaa cgtgtgtgaa gccactggtt
     6541 gtcagcgcag ggctctcggc actgaggaag cagtgaccac tatcccctat caaataacaa
     6601 ttaaatacac acagaatgcg aggcacacaa ctgagtttca ggagaggcct cgctcaggca
     6661 aggggttcaa gaggcttctg tgggacccgc tggatgttcc agggagttct taaagatggg
     6721 cgtgcctcca gccaagtgaa atcaagagaa aagtacgcga agtataggaa aactcagcag
     6781 tctggagagg taaatagggg aggaatccga ggctcagaga caggagtgac ttgcccacgg
     6841 acgcacagca agttggcagg tggagtttca gctgtgccac cttctgaagc cgggtaccct
     6901 ttacagccac cagatacaag cgggatagag acagctgatg gagaagctgg aggtgggggg
     6961 cgggaccccg aaggtgggga aagggcgcgg gggggcggtc ctatgacgta atttcctggg
     7021 tgtgtgcgcg cgtgtgcgtg cgtgtgcgtg tatataaaag ccggcatagc attgctgctg
     7081 ctgccgccgc caccgccacc atcaccgctg ttaccaccac cgctactgca gtgttcccgc
     7141 tggtgcagag ctttggtagc cagactacag acccactccc gccatcctcc tgcagcagct
     7201 cgtccactct ttccgcaccg tccggctcgc tatgcgctcc accgcggttc tggcgcttct
     7261 gctgtgcgcc gggcaaggtg agcggctccg ggctgggcag cacgatcccc tctcttgccc
     7321 caggtcccta gaggagccgg aggattcagc acctcggaca gcaccaggtc tcctgcttga
     7381 gtccaggcgc aagacccata atgttggcta tggtcgccca ccacacccga agaaactata
     7441 aagcctgacc caaccccact gggcagacca tccccaccca tgtccgcaca ccacccactg
     7501 gtgctctggc tccacgccga ccaaggtcac tggggggaga taggggactg gcgcgggctt
     7561 ctcaacccct ctcctgtcct tccaccctcc agcctgtcct tgatctcttc cctcacccct
     7621 gctctttccc gccacgtctg gggtttctcc ccttgcctgc ctctgttcct atctcagtcc
     7681 cactacatgg ctgggcctgg gcttgttttc attgaacttg atttcagctc tgtattccca
     7741 tggggaaggg ttgaggctct tcagtgcagc ctctgagccc agcccccccc cgggggtgca
     7801 gcatggaggg ggggatagca gaatcagtgc tgctaactta tttgggtttt gagggtgtgt
     7861 tggttggtgg gtcccttgag cctcccgcag ggaaggactg gggttccctc gttgcagaga
     7921 gatagcccca attagcaatc tcaagtgctc tctctctcgc tctctctctc tctctctctc
     7981 tctctctcca gtttttgccc ttcctgtgaa cagccccatg acaaaagggg acaccaaggt
     8041 aagaaggaat gttggggtac cctgggagag gaagggttcc ctgtggactc ctcagggcca
     8101 atccttgggc agctgcagga aagagtctgg catagagcca aaaggcagca ctgcagcggc
     8161 tgagcctgca caggggacag cttgcacaag aagacatcaa agatctcttg cttttgttgc
     8221 tgcccagcta cagtgacttc ccaaggggcc catgtctcac ccgatgagtc ctgggaccag
     8281 ggacactggt ctgagccatg accatagctt ctcctgccct aggtgcacag acctgcctgg
     8341 ctgtcacctc tctttgtcat tcctcttctc ctaccctcac tctgcccaga gcttcctctg
     8401 gcagtaagaa agaagccaca tcagatgcta ggaccacatt cacatcagag ccaggggtgt
     8461 tcttcctcca gagaatcgga aagttccgtt ctctatgttc ggagggctgg cttcttctgc
     8521 aagaagccac agtttaaaaa ttgtgccgtt aaagggaact caccgtttac ttgttgaatt
     8581 gtattttctt gtttatttgt tgttaactga acacaggcca gtgtttaagt tttcactact
     8641 cattctagct gtgtaacctt ggaaaattca tctctctata tctctatctc tatctctatc
     8701 tctatctcta tctctatctc tatcttcctt ccttccttcc ttccttcctc tctgtctatc
     8761 tctctttttt aaaagattta tttattgtta tatgtaagta cactgtagct gacttcagat
     8821 acaccagaat agggcatcag acctcattac ggatggttgt gagccaccat gtggttgctg
     8881 ggatttgaac tcaggacctt tggaagagca gtcagtgctc ttacctgctg agccatctct
     8941 ccatccctct ttcttccttc cttccttcct ttctttcttt ctttctttct ttctttcttt
     9001 ctttctttct ttctttcttt ctttctttct ttcttttttt gagacagggt ctctctatgc
     9061 agccatggct gtcctggaac tcattatata gacgaggctg gcttgaactc agagaaatct
     9121 accagcttct ctctcccaag tgctgggatt aaaaatatgc accacattat actgcacccc
     9181 agcattcttt tttgtttgtt tgtttgattt tggttttgtt ctagacaggg tttctctgtg
     9241 tagccctggc tgtcctggaa ctcactttgt agaccaggct ggcctcgaac tcagaaatcc
     9301 acctgcctct gcctccggag tgctgggatt aaaggcatgc accaccacgc ccggctgcac
     9361 cccagcattc ttaaggccct tacccaactc ctgtctcttg gtgtctgccc tcaaaggctg
     9421 gtggttgcca agtattctcc atgtaaagtc tagtgttagg gagacagtac atgcttctag
     9481 gagatactta cctggtccac tccacaccat agtatgggag caggggtcag gggtgccagc
     9541 aggtaaggaa gacctgtatg ctccctttga tgctgctatg actacatacc ctgacaacct
     9601 ttgccagctt gctatggcat cccatagaca cagcctccca caccttcact ccgtgctagt
     9661 gttgcctgac gcatgtctgg cacctcctgc ttctccaccc agctgcaggc tgtcgaagca
     9721 ggtctgatgt gattccctcc ctgagtgagc aagcgagcct gcgtggcact tcatagtggg
     9781 cagagtgttt tgcattgggg aggagttctc atcctgcttt acatgagagg aatccaaggc
     9841 tcagagagac taagtcactt gctcaaggtc acacagctag cataccacac aacagtggac
     9901 accaacttca gaaattatcc atggtgcttt gtgatttgct gctcacctca cagggtcgga
     9961 taaaatattc agaggtgtgt cgtgttggtc tggaccagac atccagctgt aactagttag
    10021 tatccacatc caaccagggg attcttcccg tgaaaacgtg gagccacaca ctgtggctgt
    10081 agaatgggag gtggtcctgt ttctggcaag ttcaaatcca ggtaggttcg tgttcttcaa
    10141 tgggcagtct ggaggaactg accctatgga ctctgctaga ccccagtcct ttgcaagagg
    10201 tccttttagg tctcagaggc agcgccatcc ttggagaact accttacgag tgagaacatg
    10261 ttaaggcgca taacgatacc acgatatcaa caagtttgta caaaaaagca ggctggcgcc
    10321 ggaaccgaag ttcctattcc gaagttccta ttctctagaa agtataggaa cttcgaaccc
    10381 tttcccacac caccctccac acttgcccca aacactgcca actatgtagg aggaaggggt
    10441 tgggactaac agaagaaccc gttgtgggga agctgttggg agggtcactt tatgttcttg
    10501 cccaaggtca gttgggtggc ctgcttctga tgaggtggtc ccaaggtctg gggtagaagg
    10561 tgagagggac aggccaccaa ggtcagcccc ccccccctat cccataggag ccaggtccct
    10621 ctcctggaca ggaagactga aggggagatg ccagagactc agtgaagcct ggggtaccct
    10681 attggagtcc ttcaaggaaa caaacttggc ctcaccaggc ctcagccttg gctcctcctg
    10741 ggaactctac tgcccttggg atccccttgt agttgtgggt tacataggaa gggggacggg
    10801 attccccttg actggctagc ctactctttt cttcagtctt ctccatctcc tctcacctgt
    10861 ctctcgaccc tttccctagg atagacttgg aaaaagataa ggggagaaaa caaatgcaaa
    10921 cgaggccaga aagattttgg ctgggcattc cttccgctag cttttattgg gatcccctag
    10981 tttgtgatag gccttttagc tacatctgcc aatccatctc attttcacac acacacacac
    11041 cactttcctt ctggtcagtg ggcacatgtc cagcctcaag tttatatcac cacccccaat
    11101 gcccaacact tgtatggcct tgggcgggtc atcccccccc ccacccccag tatctgcaac
    11161 ctcaagctag cttgggtgcg ttggttgtgg ataagtagct agactccagc aaccagtaac
    11221 ctctgccctt tctcctccat gacaaccagg ctcccaggtc ccgaaaacca aagaagaaga
    11281 acgcagatct ctagagtcac cgagttgctt taccggatga agagggccga aacatactgt
    11341 ccaaggccct tgctggcaat ccacccaact gaagccagac acaaacagaa aattgtggca
    11401 ccggtgaaac agactttgaa ttttgacctt ctcaagttgg cgggagacgt cgagtccaac
    11461 cccggaccgg tgcatgtgga ccccaagaaa aagcggaagg tggaccctaa aaagaaacgc
    11521 aaagtggatc ctaagaaaaa aagaaaggtg cacgtgtcca tggtgtccaa gggcgaggaa
    11581 ctgttcaccg gcgtggtgcc catcctggtg gaactggatg gcgacgtgaa cggccacaag
    11641 ttcagcgtgt ccggcgaggg cgaaggcgac gccacatacg gaaagctgac cctgaagttc
    11701 atctgcacca ccggcaagct gcccgtgcct tggcctaccc tcgtgaccac actgacctac
    11761 ggcgtgcagt gcttcagcag ataccccgac catatgaagc agcacgactt cttcaagagc
    11821 gccatgcccg agggctacgt gcaggaaaga accatcttct ttaaggacga cggcaactac
    11881 aagaccaggg ccgaagtgaa gttcgagggc gacaccctcg tgaacagaat cgagctgaag
    11941 ggcatcgact tcaaagagga cggcaacatc ctgggccaca agctggagta caactacaac
    12001 agccacaacg tgtacatcat ggccgacaag cagaaaaacg gcatcaaagt gaacttcaag
    12061 atccggcaca acatcgagga cggctccgtg cagctggccg accactacca gcagaacacc
    12121 cctatcggcg acggccctgt gctgctgcct gacaaccact acctgagcac ccagagcgcc
    12181 ctgagcaagg accccaacga gaagagggac cacatggtgc tgctggaatt cgtgaccgcc
    12241 gctggcatca ccctgggcat ggacgagctg tacaagcctg caggcgaggg cagaggaagt
    12301 cttctaacat gcggtgacgt ggaggagaat cccgggccgt ccaatttact gaccgtacac
    12361 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
    12421 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
    12481 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
    12541 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaactatc
    12601 cagcaacatt tgggccagct aaacatgctt catcgtcggt ctgggctgcc acgaccaagt
    12661 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
    12721 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
    12781 atggaaaata gcgaccgctg ccaggatata aggaatctgg catttctggg gattgcttat
    12841 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
    12901 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
    12961 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
    13021 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
    13081 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
    13141 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
    13201 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
    13261 atcatgcaag ctggtggctg gaccaatgta aatattgtca tgaactatat ccgtaacctg
    13321 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgatctcga gccatctgct
    13381 ggagacatga gagctgccaa cctttggcca agcccgctca tgatcaaacg ctctaagaag
    13441 aacagcctgg ccttgtccct gacggccgac cagatggtca gtgccttgtt ggatgctgag
    13501 ccccccatac tctattccga gtatgatcct accagaccct tcagtgaagc ttcgatgatg
    13561 ggcttactga ccaacctggc agacagggag ctggttcaca tgatcaactg ggcgaagagg
    13621 gtgccaggct ttgtggattt gaccctccat gatcaggtcc accttctaga atgtgcctgg
    13681 ctagagatcc tgatgattgg tctcgtctgg cgctccatgg agcacccagt gaagctactg
    13741 tttgctccta acttgctctt ggacaggaac cagggaaaat gtgtagaggg catggtggag
    13801 atcttcgaca tgctgctggc tacatcatct cggttccgca tgatgaatct gcagggagag
    13861 gagtttgtgt gcctcaaatc tattattttg cttaattctg gagtgtacac atttctgtcc
    13921 agcaccctga agtctctgga agagaaggac catatccacc gagtcctgga caagatcaca
    13981 gacactttga tccacctgat ggccaaggca ggcctgaccc tgcagcagca gcaccagcgg
    14041 ctggcccagc tcctcctcat cctctcccac atcaggcaca tgagtaacaa aggcatggag
    14101 catctgtaca gcatgaagtg caagaacgtg gtgcccctct atgacctgct gctggaggcg
    14161 gcggacgccc accgcctaca tgcgcccact agccgtggag gggcatccgt ggaggagacg
    14221 gaccaaagcc acttggccac tgcgggctct acttcatcgc attccttgca aaagtattac
    14281 atcacggggg aggcagaggg tttccctgcc acagcttgat gaagatctga gctcttaatt
    14341 aatgatcata atcagccata tcacatctgt agaggtttta cttgctttaa aaaacctccc
    14401 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
    14461 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
    14521 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
    14581 gatccgttta aactaacttt aaataattgg cattatttaa agttacatat ggcggccgcc
    14641 ctaaggctcg acctcgaaat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg
    14701 agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg
    14761 cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc
    14821 gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg
    14881 caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc
    14941 tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc
    15001 tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag
    15061 gggcggggcg ggcgcccgaa ggtcttccgg aggcccggca ttctgcacgc ttcaaaagcg
    15121 cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct gcatccatct
    15181 agatctcgag cagctgaagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac
    15241 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
    15301 cacgcgccac accgtcgatc cagatcgcca catcgagcgg gtcaccgagc tgcaagaact
    15361 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
    15421 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
    15481 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
    15541 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
    15601 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
    15661 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
    15721 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
    15781 catgacccgc aagcccggtg cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag
    15841 gagcgcacga ccccatgcat cgatgatatc agatccccgg gatgcagaaa ttgatgatct
    15901 attaaacaat aaagatgtcc actaaaatgg aagtttttcc tgtcatactt tgttaagaag
    15961 ggtgagaaca gagtacctac attttgaatg gaaggattgg agctacgggg gtgggggtgg
    16021 ggtgggatta gataaatgcn tgctctttac tgaaggctct ttactattgc tttatgataa
    16081 tgtttcatag ttggatatca taatttaaac aagcaaaacc aaattaaggg ccagctcatt
    16141 cctcccactc atgatctata gatctataga tctctcgtgg gatcattgtt tttctcttga
    16201 ttcccacttt gtggttctaa gtactgtggt ttccaaatgt gtcagtttca tagcctgaag
    16261 aacgagatca gcagcctctg ttccacatac acttcattct cagtattgtt ttgccaagtt
    16321 ctaattccat cagaagctgg tcgagtaact ttaaataatt ggcattattt aaagttaggc
    16381 gcgccataac ttcgtatagc atacattata cgaagttatg tcgagatatt taaatattag
    16441 acccagcttt cttgtacaaa gtggttgata tctctatagt cgcagtaggc ggaggcacct
    16501 gctcccaccc ctcctttagc agtggttccc tcaagacaca tgctggaatg cccatgtgat
    16561 ttgactggcc cacagtaagc agtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg
    16621 tggtgtgtgt gtagtgtcct gtttctgtgg ctctatggtc ttattaaaga agcagagctg
    16681 ggccccatgg ccccttgaag acaacacaaa tgctcttctg cccttctggg aaagttgccc
    16741 tggcacctca ggggctgagc tccatgttca caccggccac agcccacctt gctgtctccc
    16801 aggtgatgaa gtgcgtcctg gaagtcatct ccgactcgct gtccaaaccc agccccatgc
    16861 ctgtcagccc tgagtgtctg gagaccctcc aaggaggtag gagtcagagg ctggaggagg
    16921 aattgggagg ccagggtgtg ggagggtagc tcttaatcat cacatccagt actcattgcc
    16981 ccagtgccct ggtatgtgct agaatctggg gaagacacag gaatgggtga cagctcctgg
    17041 gaccctgagg tttgtagaca ctcagaggga catatggcag ttaactagaa gcctgggcag
    17101 aatgaaggag ctggaacagc aaggtgcaga ctgggtgccc tgcgtactag ggttctgtgt
    17161 tctgtactct cagtgtttgg agtctcgctg acaccagtag ccgttctctg gatctgggat
    17221 gaattcagtg ctgagtgggc ctaaggagga ccacatcttt ccaaacagac agcccagttt
    17281 gtctagcgca tgatagcgct gggacaggta gaaaatgagg aaggatgtca ggaggaacag
    17341 acaacaggca gcatggaaaa gggaaaggaa gaatttacac actcactgga gtctgaggtc
    17401 cacagtccct tccttggagg ctgggaccag agtcagcagg tgacctttct gctgcagtag
    17461 gaaggtgatt gacatcttgg aaatgaggaa tttccaatgc gttctaccaa gatccaggct
    17521 tgccccctgg tgccaagttt ccagaataaa tgcttgagct cagctgaaaa tgtccacact
    17581 aatctcttct gagaagccac cagtagccat gctttctccc tgcagacgag aggatcctct
    17641 ctatcctgcg acaccagaat ctgctgaagg aacttcaaga cctggctctc caaggtattt
    17701 cagcagcata cgcagttcca cacagaaaag ccagcaacag agcctgaggg gacctgggtg
    17761 gaggctgagt gtactcttcc aggtgctcag ttccagagga gatggcgcaa cgcaattaat
    17821 gataacttcg tatagcatac attatacgaa gttatggtct gagctcgcca tcagttcaca
    17881 gaaaggcggg gtcctctgtg tcacctggct ccattgtcta gagactaaga gctcaaggct
    17941 cagtgaggct cagggattcc ctcagggtca cagtgagtag atcttgttaa ggacagatcc
    18001 aggtctgctg atgttcctcc gccatccagg gaggccgaga gagctcttct ctgcagaccc
    18061 agaaatgaac tcacacagag agaagacttt catttcctgc gactctttgt tctatgggta
    18121 atgcttgccg ataaacacag agcagatgag tgtgtttcgg actgagaggt aacttgcccc
    18181 caagagatta agaaaacagt agacaggcag tggtggcaca tacctttaat ccgagttaag
    18241 ccagctgatc tacagaatga gttctgggac agccagagct acacagagaa accctgttgc
    18301 aaaaaaacaa agaaagaaag taaaaatcaa aactaatgaa caaacaaaca atcaatacaa
    18361 aaaagaaaga aaggaaggaa ggaaggaagg aagaaagaaa gaaagtccac agatactttt
    18421 cagacaggag tgtgaggctg gggtctcata gcattgaaga tatacaagct agacagcggc
    18481 taggatggag atgttcttag gctgcagtct cccacccttc ctgtttgagg tcgtgctata
    18541 gattgaaccc agggcttcat gcgtgcttgg caagcacccc accaaatgaa gcccagctcc
    18601 agttctatgg catattcatg gtatctcctg gcacacggag ggtggtgggt ggtatagggg
    18661 ttgttataat caattttgtc ctggctttgt aaacacaaac aaggtctcat tgtgtagcca
    18721 cactgtcctg actctgcagt cctgcctcag ccttttgagt gctgggattg caggcatgag
    18781 ccaccacacc caccatgcca ggtccaggta caagacactg tgggtctgcc ccttaaacat
    18841 gcccactttc ctcaggaggc atccagggta gaagcagggc tggatgccag gcgatccggt
    18901 ctccggggtg gcacttttta aacaaaacat tagatatttt aatgataaat ttaaaattaa
    18961 ctattgggca gatattctat ttaaataaat aagtaattca atgagaccat tagagaggag
    19021 tgtgctctat tctacgttct cacaagtctg gatctaacca tgcacagcat ccagtgcaca
    19081 cagggttggt ttccgttatg aggatactgg gtgtgtcttc agctacatca tcatcacccc
    19141 attccctcct ggtttctata taatagcctt gcatggagac ctccctccac tttaacatct
    19201 accacaagtc tgtttataac cttggggagc ccatgttacc caaggtcaca gagctatagg
    19261 catggccagg gcaggcctgg agctcactgt cgctgggttg ggcttatttc ctagcctaca
    19321 gtgaccccac agctgcagag ctgtgtcctg attcagacac ggctgcagga tactcagggc
    19381 aacatctgca gcaatagctg caggggttag gttggcagag ggactctggg caatgtaggt
    19441 ataaccctgg gctcaaaggt agacatagca cccacctttt cctggggcca gacctggagt
    19501 tccagaacag tggaggctgt ttaacaaaag agcacccatg agggcgtgct gccctggaga
    19561 tctgtctgtc ccatttgggc ctttattgct ctctaaggta caagtagaac tttctcacat
    19621 ttcccactag ggacactgag tcagaggggt ggtgtggcct gatgggtata gctgagagcc
    19681 tgtaccctca agggaggcct tcagtgttac ggacaggtat gtcaggtcca catggaacct
    19741 tcccttgtcc cctcctacaa ggtgccaagg agcgggccca gcagccgctg aagcagcagc
    19801 agccgccgaa gcagcagcag cagcagcagc agcagcagca gcaggagcag caacacagca
    19861 gctttgagga tgaactctca gaagtgtttg agaaccagag ccctgatgcc aagcacagag
    19921 gttagtgtgg ccagctaggg gggaagggag ctacaaggag aacagtagtc cccaggtgat
    19981 gctgcttctt cacagcagga agtcagctac cattgaactt ggcctcagga atcccctagg
    20041 gcatccgggt cctctgcttg accctatcca tttgtgtttg cagacgcagc agcagaagtg
    20101 ccctccaggg acactatgga gaagagaaag gattctgaca agggacaaca ggatggcttt
    20161 gaggcaacca cagagggacc taggcctcag gcctttccag agcctaacca agagtccccc
    20221 atgatgggag acagtgagtc ccctggggag gacacagcca ccaataccca atcaccaacc
    20281 agcctcccca gccaggagca tgtggaccca caggccacgg gagacagtga gagaggcctg
    20341 agtgcccaac agcaagccag aaaagccaag caagaggaga aagaagagga ggaggaagag
    20401 gaggctgtgg ctagagagaa ggctgggcct gaagaagtcc ccactgcagc atccagttcc
    20461 cacttccatg caggctacaa agcgatccag aaagatgatg gtatgtatgg ggacagggac
    20521 ctcaactgat gtgtctggga gatgggtggg tagggacttc atgccaacct cataataatc
    20581 ctgaaagatg aatagcatct ccactgtgtg tgtgtgtgtg tggggggggg tagggagcaa
    20641 gaggctcagc gaggttatgt aatttgccta aggctacaca gctaattaaa gacaagtgta
    20701 gtttcaagcc cagtttatct gactcctaaa ccaagtgtca accactcctc tacacccggg
    20761 agcaagagga gcttaggggg caggtgttcc tcttagagaa gggagagaca acagggtgag
    20821 cttcagtgag gggctctgcc aactagctcc tttgaggttt gcagggtctt gcttcatgct
    20881 ccccagtgga gctggctgga tcctgacggt gagcttggct ttagaggtgg ctgcacaaaa
    20941 gaccttgagg agtggcaggg gctggggaaa tggcactcct tcctcacttc ctgttcctgc
    21001 cacacctgct ccaggtcagt cggactctca ggcagtggat ggagatggaa agacagaggc
    21061 ttccgaagct ctgccgtctg aagggaaggg ggagctggag cactctcagc aggaggaaga
    21121 tggggaagag gccatggtag ggacccccca aggtctcttc ccccaaggcg ggaagggccg
    21181 ggagctggaa cataagcagg aggaagagga agaggaggag gagcgtctgt ccagagaatg
    21241 ggaggacaag cgatggagca ggatggacca gctggccaaa gagctgacag cagagaagcg
    21301 gctggagggc gaggatgacc ctgaccgctc catgaagctc tccttccgga cccgcgccta
    21361 tggcttcaga gatcctgggc ctcagctacg caggggctgg aggccatctt ccagagaaga
    21421 cagtgtggag gcccgaagtg actttgagga aaagaaggaa gaggagggca gcgccaaccg
    21481 cagagcagag gttggtatag gcaagggcag ccacccccag aagtccctct cccactgagg
    21541 tgacgtggtt cccttaggtg ggtgctggac ttgggatagg gggaacaact ggcttatagc
    21601 aggggctcaa cagagttact gggtcaggtg gacagtggga aatggctgga actggaggcc
    21661 tcagacctgc ctgagggaca cggagtgggg gcattgtctc tgctggtatg tgagcaggac
    21721 acgggagtga tagggtcagg cctggtgctt tggatggaga gcttggcagg acttgcctgg
    21781 ggcctctcag gacagacacc tctggtccca ggaactggga acggatgcta gggggaccag
    21841 tggtgaccga ggaagacagc ctggttgatg gccatgtctt cctggtcccc agggaactca
    21901 attgtgcctc ctcctgggtt taaactcaca gaggccacaa tggcatagca gggcgagcag
    21961 atagggagtc atggaggtgc tggcagattt gggttccagc tggccctggc tgcgtgactt
    22021 tgggcaggcc attacccctc tctgggtctc acttgttccc ctttgtgaag tggaagtatg
    22081 ggagttcctc taacttacct tatcataatc agaacatgac cttgaaaacc ctactatgcc
    22141 cagggccact t
//