LOCUS       239330#pL1L2_GT0_LF2A_nEGFPO_T2A_CreERT_puro#L3L4_pD223_DTA_T_spec        20400 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT0_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 239330
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000042755
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            4729..4880
                     /db_xref="ENSEMBL:ENSMUSE00000390049"
                     /rank="1"
                     /note="ENSMUSE00000390049"
     exon            5713..5764
                     /db_xref="ENSEMBL:ENSMUSE00000325553"
                     /rank="2"
                     /note="ENSMUSE00000325553"
     exon            6665..6785
                     /db_xref="ENSEMBL:ENSMUSE00000325547"
                     /rank="3"
                     /note="ENSMUSE00000325547"
     misc_feature    4275..9496
                     /note="5 arm"
     misc_feature    9524..9548
                     /note="B1 Gateway"
     misc_feature    9561..9608
                     /note="Frt"
     misc_feature    9609..10108
                     /note="mouse En2 intron"
     primer          complement(9677..9698)
                     /note="LRPCR LAR3_EN2"
     primer          complement(9573..9596)
                     /note="FRT3"
     primer          13760..13778
                     /note="seq primer NF"
     misc_feature    13820..13827
                     /note="RE sites"
     misc_feature    15620..15653
                     /note="loxP"
     misc_feature    complement(13828..13859)
                     /note="Rox"
     misc_feature    complement(15580..15611)
                     /note="Rox"
     misc_feature    13860..13881
                     /note="polylinker"
     CDS             14440..15036
                     /note="puromycin N-acetyl transferase"
     misc_feature    14555..14560
                     /note="Mutated RsrII site"
     intron          10109..10483
                     /note="mouse En2 intron"
     misc_feature    10481..10483
                     /note="Splice Acceptor"
     exon            10484..10523
                     /note="mouse En2 exon"
     primer          10384..10405
                     /note="seq primer FCHK2"
     misc_feature    10524..10646
                     /note="1Dleader"
     misc_feature    10647..10698
                     /note="F2A"
     misc_feature    10794..11510
                     /note="EGFP"
     misc_feature    10704..10793
                     /note="3X NLS"
     misc_feature    13576..13814
                     /note="SV40 polyadenylation site"
     misc_feature    12609..13553
                     /note="ERT2"
     promoter        13892..14403
                     /note="PGK promoter"
     misc_feature    15147..15579
                     /note="PGK pA"
     primer          13634..13657
                     /note="LRPCR RAF5"
     misc_feature    11520..11573
                     /note="T2A"
     misc_feature    11570..11570
                     /note=" A to G to kill RsRII site"
     misc_feature    11574..12599
                     /note="Cre"
     misc_feature    10499..10524
                     /note="FCHK lf2a_lacz_gt0"
     misc_feature    10499..10514
                     /note="FCHK QC prefix region"
     misc_feature    10515..10518
                     /note="FCHK QC critical region"
     misc_feature    10519..10524
                     /note="FCHK QC suffix region"
     misc_feature    15676..15696
                     /note="B2 Gateway"
     misc_feature    9497..15726
                     /note="Synthetic Cassette"
     exon            16124..16335
                     /db_xref="ENSEMBL:ENSMUSE00000325541"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 1 ENSMUSE00000325541"
     misc_feature    15727..16554
                     /note="Critical Region"
     misc_feature    16578..16611
                     /note="Downstream LoxP"
     misc_feature    16555..16634
                     /note="synthetic loxP region"
     exon            17380..17512
                     /db_xref="ENSEMBL:ENSMUSE00000325531"
                     /rank="5"
                     /note="ENSMUSE00000325531"
     exon            18448..18545
                     /db_xref="ENSEMBL:ENSMUSE00000325522"
                     /rank="6"
                     /note="ENSMUSE00000325522"
     exon            19783..19912
                     /db_xref="ENSEMBL:ENSMUSE00000325515"
                     /rank="7"
                     /note="ENSMUSE00000325515"
     misc_feature    16635..20400
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggaaaatat acttgaccga tggttttgag cgaaataata actggataat
     4321 caagaaatat atccactaat gaatagcctg aactactgaa acaatttgtt cagtgcctag
     4381 catatggtgt gcattttatt atttctttca aaaagaatgt atttggagtt acatagtaag
     4441 tctgctacct tttctttatg gctatatcta tgtcttatgt tgagatgaat gaattattct
     4501 tcaggggaaa taatctattt gaacagttta gatggtgaag aacatttgca gcatttgcaa
     4561 gatttttttc cactctgaag tggtctttgt ccttgaacat aggatacaag tgacccctgc
     4621 tctgttaatt attggcaaat tgcctaactt caacgtaagg aaatagagtc atatgtttgc
     4681 tcactgaagg ttactagtta acaggcatcc cttaaacagg atataaaagg acttcagcag
     4741 gactgctcga aacatcccac ttccagcact gcctgcggtg aaggaaccag cagccatgaa
     4801 gtggatcaca cccgcttccc tcatcctcct gctacatttc gctgcgtcca aagcattgca
     4861 cgaaaatgag tttgggatag gtaagatgct ttgtgacttg ggagctattt tcctataaca
     4921 aaattcttaa aaattcccat atttgcatct ccaggtatct tttttttttt tttttttttt
     4981 tttttttgag acagggtttc tctgtgtagc cctggctgtc ctggagctca ctttgtagac
     5041 caggctggcc tcgaactcag aaatccgcct gcctctgcct cccgagtgct gggattaaag
     5101 gtgtgcgcca ccacacccgg ctctccaggt atcttgactt aagcattcta tatactgttg
     5161 aagaccaggt atagatctaa gtggtagata gatttagatg taatgtaatt ttatgtaaga
     5221 ggcttataaa ataacagcag tgttcaacac aatcaatttt ggatgtgttc catgctcaat
     5281 ttttttttga tattaattat ttagtagggt ctcatgtcta gttccttcgg gttgacagaa
     5341 ataacatttt cactctcaat ctttttaaaa gtgctaattg ttcgactctc tggaaagcct
     5401 aatgtgaaca ggccgactgt agcataatta tgtccaaagc tactaaatga agccattttg
     5461 aatattatac tgtgagggag ccaagtagta aggggacctg aaagatgagt attagtacta
     5521 agtctgcaac tgatcattct ggttaacttt gttccttaat ttttcagtgt ccccaagtgt
     5581 aaaattaaga tgtctggaca ggtggttcct aacaacttgg cagttctaag acttcatggc
     5641 ttgggtttaa attccacaac gattgctttc ccggttaaaa tcctcacttg tccaagtctg
     5701 ttttcctttc agcttccacg ttagattcct cccagtgcgt gacggagaag aatgtgctta
     5761 gcatgtaagt ttcgcttcca tcaatgtgct gcagaaatgg cgtgcagaga taagtctagg
     5821 caaccgaatg tgaggtttga aattacagga gcaaattggg cagctttaag gctttgcttg
     5881 tgctgaatat cacactgata agcaacatgt ttaacttcgg tcaacgcttt agatcttagc
     5941 cttgacattg actggagttc cagaaagcaa gccaaatcag attttcaaaa ctgaagaatt
     6001 aatttctctg cagctttgta tgggctcagg agcgtgtctt gtcagaacca ataggaagat
     6061 attgcgatat cctctatttt gtaagctctt taatatcaag tcataggaag taaaatattc
     6121 ttagtttagt tattgtggag aatattttat tcctttaaat attgtctctg ttctacttct
     6181 ttgacattta tagtaaggaa tttaaacgtg tgtcactggt aatgtctgca tgtagaaagc
     6241 tggacacgga tcactttgag tcaatcggac actagtgcaa tctaggattg gggctcagtc
     6301 acgctgggag caaatataac tgaaggtcca gtgaccttca tctttgctta aattaagtaa
     6361 gaaaaatagt gtgaaaaatg tgggccttcc caggtataga tgctcagtga gcagtgaccc
     6421 caaaagagga agtatatttg ggggaaatct atttattttg acttttaaga cttgcacctg
     6481 tatttatggg tgaagatgac agaaagaagg ctagcatttc cctctgagtg actcgaaccc
     6541 agtttcccgt agatctttag caaagggctc cttctaaaca gatcagagca gaggaagctg
     6601 tacttgaaag gcacgcgtga ctgggtctca gcctcaagcc gctctgcatg tggtctcctt
     6661 gcagagctac catcaccttt acccagtttg ttccggaagc caccgaggag gaagtgaaca
     6721 aaatgactag cgatgtgttg gctgcaatga agaaaaactc tggcgatggg tgtttagaaa
     6781 gccaggtgag tgattagaga gaaagccaca ttgacactta accaaaattt aggctgcaga
     6841 aagatgtcag gaagttgtgg gtttaattat ctgaaaactg ttttctggaa gtagggttcg
     6901 attagttctg gaatcccgcc gaacagcact gttgaagcag agcaagcggc tgcttaaagg
     6961 agctgagatt aatacgagac tcagagctaa ggctccggag taacgctatc tgagcaacca
     7021 caaacacatt ctcttctttg agcttccata cctgcaatta tggagtgaca ttagatccac
     7081 tcagagctgt cacggaggtt tatgttgatg acgagtggca gtatattgcc tcttatgcag
     7141 acattgtggc tgacagcatt ctagggatca ctgcaaacac actgataatc aaaacgctgc
     7201 atggaaggtg acacccgcag aaaatctgtc cagtcaacta agagaaattg gctgcagcgt
     7261 atcatcagaa ttcctatctt atttataaat gttaatttat taaattataa tatttatatt
     7321 ttatacatat attacagttt acatactatg ttatattata tatggagaca tagtacatat
     7381 tatattgtat attatatatt acatattata ttatatatga acaatccata tttgtcatgt
     7441 agttataatt ttatatagtg ttatatcaca ttatacaata ataatttaat taattgacag
     7501 attaattaaa tctctatgat aatcctggaa agttgaggac aattttctca tggaggaaac
     7561 ttaggaacag ggaaatcact cctttggaga attatagttt ggtagacctg aggattaaat
     7621 gaagtcttct ggctccagaa ttaggtcctt agcaaggtgc tcacactgtt cagaacagca
     7681 cggtgagtgt gaggcctgca gcaggttatt cgagttgctg gatatagcac gtacgcagac
     7741 aggaacatca gtgtctgata tccaagtaag tcagaacttg agaaaaacaa gcacgtttac
     7801 acacccagtt gccctggtat agaaacaaaa tagctaccat tctgtagttt actcctgtat
     7861 ggaaatgtgc tttatacttg ttggagggat ttaagtatgg aatacataca gcagagcatg
     7921 ggcacagggc cttccgacgg tgcatatgcc aagggccgca ccctaaagaa agccactctt
     7981 taccttccag cagccagcaa ctgcccgtca ctcctcggca aggacccagg gtttgtgagc
     8041 ccttctccat ccacacaggc gttttgtgta caattgcctt gttatgtatg aaaagttttt
     8101 gcttcaatct tcctgcagtc tctggccctt aaaatccttt ctcccttctt ctccaatgga
     8161 ccctgattct tgaggggaga gagagagcct ttcctttctg tagacatccc atttggggct
     8221 gagcacatca tggctactta tcctctgtat tttggccagt tttgaacctc tgcatccatt
     8281 accatcccct gcaaatagag cctcctggga tgaagactgg ggctgcacaa agttctgcca
     8341 ctctgtgttt agaaggcact ctagtattat gcccattcag taaaacaaat agagataggt
     8401 tcactcttag ggcctatacc atactcagcc atgggttctt ggcccagctt gcagtgccag
     8461 gcctgagtgc caccctgtca gagagtactg tttgtcactc tcaggaatct atgtcactgt
     8521 tgtatcaatg gctatacctt agccaggctg actgtagctc ttagggttta cagctggggt
     8581 aaccccgtag atgacatcac atgacttttc tccccctagc ggtttgcaca tgcccttctg
     8641 gaattatgaa atctagccag taagaaggca acctcagacg cttctggctc tagaatttat
     8701 gggttaataa gaatctccgg tttgtatgtt agtttcagtc actctttgtt agtcagttaa
     8761 aaaggcgtgt ataaattatg cccaaagcac ttcttattgc gcctttcgta ttcagttagt
     8821 gttcagggta atgttgcaaa tggggtggct cgtcactaca agttcaaaag gtttctttta
     8881 aaaggttgac tgattctgta gccagaagat ggcctagtcg gccatcattg ggaagacagg
     8941 ccccttggtt ttgcaaactt tatagcctca gtacagggga ccgccagggc caagaagtgg
     9001 gagtgtgtgg gtaggggagt ggggggggga gggtatgggg aacttttggg atagcatttg
     9061 aaatgtaaat gaagaaaata cctaatttta aaaaagacaa taataaaaca aacaaacaaa
     9121 caaaacccaa ccaaccaacc aaataaaaaa aggttgcgtg aaacaaacat ggcggtgcac
     9181 cactatggtc tcagggcttg ggaggctgag gtaagtggat ctgagtttgg ggcgagtctt
     9241 gtttccatcg tgagtttcag gctagccgtg acttcatcag gaagaaaccc tatctcatcc
     9301 tcgtagagaa tatcacaaac caaaaatgaa tatagagaat gagtcttcag taagcctgtt
     9361 gtaatagtta aataaagcga ttcctaaggt gggtgacttc cttcaaaata ctggtcctgt
     9421 ccaaatctca gtgaatttag gtgctaagga agaaagcatt ttaaaatacc gtagctaaat
     9481 ctgtaattat gactaaaagg cgcataacga taccacgata tcaacaagtt tgtacaaaaa
     9541 agcaggctgg cgccggaacc gaagttccta ttccgaagtt cctattctct agaaagtata
     9601 ggaacttcga accctttccc acaccaccct ccacacttgc cccaaacact gccaactatg
     9661 taggaggaag gggttgggac taacagaaga acccgttgtg gggaagctgt tgggagggtc
     9721 actttatgtt cttgcccaag gtcagttggg tggcctgctt ctgatgaggt ggtcccaagg
     9781 tctggggtag aaggtgagag ggacaggcca ccaaggtcag cccccccccc ctatcccata
     9841 ggagccaggt ccctctcctg gacaggaaga ctgaagggga gatgccagag actcagtgaa
     9901 gcctggggta ccctattgga gtccttcaag gaaacaaact tggcctcacc aggcctcagc
     9961 cttggctcct cctgggaact ctactgccct tgggatcccc ttgtagttgt gggttacata
    10021 ggaaggggga cgggattccc cttgactggc tagcctactc ttttcttcag tcttctccat
    10081 ctcctctcac ctgtctctcg accctttccc taggatagac ttggaaaaag ataaggggag
    10141 aaaacaaatg caaacgaggc cagaaagatt ttggctgggc attccttccg ctagctttta
    10201 ttgggatccc ctagtttgtg ataggccttt tagctacatc tgccaatcca tctcattttc
    10261 acacacacac acaccacttt ccttctggtc agtgggcaca tgtccagcct caagtttata
    10321 tcaccacccc caatgcccaa cacttgtatg gccttgggcg ggtcatcccc ccccccaccc
    10381 ccagtatctg caacctcaag ctagcttggg tgcgttggtt gtggataagt agctagactc
    10441 cagcaaccag taacctctgc cctttctcct ccatgacaac caggctccca ggtcccgaaa
    10501 accaaagaag aagaacgcag atctctagag tcaccgagtt gctttaccgg atgaagaggg
    10561 ccgaaacata ctgtccaagg cccttgctgg caatccaccc aactgaagcc agacacaaac
    10621 agaaaattgt ggcaccggtg aaacagactt tgaattttga ccttctcaag ttggcgggag
    10681 acgtcgagtc caaccccgga ccggtgcatg tggaccccaa gaaaaagcgg aaggtggacc
    10741 ctaaaaagaa acgcaaagtg gatcctaaga aaaaaagaaa ggtgcacgtg tccatggtgt
    10801 ccaagggcga ggaactgttc accggcgtgg tgcccatcct ggtggaactg gatggcgacg
    10861 tgaacggcca caagttcagc gtgtccggcg agggcgaagg cgacgccaca tacggaaagc
    10921 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccttggcct accctcgtga
    10981 ccacactgac ctacggcgtg cagtgcttca gcagataccc cgaccatatg aagcagcacg
    11041 acttcttcaa gagcgccatg cccgagggct acgtgcagga aagaaccatc ttctttaagg
    11101 acgacggcaa ctacaagacc agggccgaag tgaagttcga gggcgacacc ctcgtgaaca
    11161 gaatcgagct gaagggcatc gacttcaaag aggacggcaa catcctgggc cacaagctgg
    11221 agtacaacta caacagccac aacgtgtaca tcatggccga caagcagaaa aacggcatca
    11281 aagtgaactt caagatccgg cacaacatcg aggacggctc cgtgcagctg gccgaccact
    11341 accagcagaa cacccctatc ggcgacggcc ctgtgctgct gcctgacaac cactacctga
    11401 gcacccagag cgccctgagc aaggacccca acgagaagag ggaccacatg gtgctgctgg
    11461 aattcgtgac cgccgctggc atcaccctgg gcatggacga gctgtacaag cctgcaggcg
    11521 agggcagagg aagtcttcta acatgcggtg acgtggagga gaatcccggg ccgtccaatt
    11581 tactgaccgt acaccaaaat ttgcctgcat taccggtcga tgcaacgagt gatgaggttc
    11641 gcaagaacct gatggacatg ttcagggatc gccaggcgtt ttctgagcat acctggaaaa
    11701 tgcttctgtc cgtttgccgg tcgtgggcgg catggtgcaa gttgaataac cggaaatggt
    11761 ttcccgcaga acctgaagat gttcgcgatt atcttctata tcttcaggcg cgcggtctgg
    11821 cagtaaaaac tatccagcaa catttgggcc agctaaacat gcttcatcgt cggtctgggc
    11881 tgccacgacc aagtgacagc aatgctgttt cactggttat gcggcggatc cgaaaagaaa
    11941 acgttgatgc cggtgaacgt gcaaaacagg ctctagcgtt cgaacgcact gatttcgacc
    12001 aggttcgttc actcatggaa aatagcgacc gctgccagga tataaggaat ctggcatttc
    12061 tggggattgc ttataacacc ctgttacgta tagccgaaat tgccaggatc agggttaaag
    12121 atatctcacg tactgacggt gggagaatgt taatccatat tggcagaacg aaaacgctgg
    12181 ttagcaccgc aggtgtagag aaggcactta gcctgggggt aactaaactg gtcgagcgat
    12241 ggatttccgt ctctggtgta gctgatgatc cgaataacta cctgttttgc cgggtcagaa
    12301 aaaatggtgt tgccgcgcca tctgccacca gccagctatc aactcgcgcc ctggaaggga
    12361 tttttgaagc aactcatcga ttgatttacg gcgctaagga tgactctggt cagagatacc
    12421 tggcctggtc tggacacagt gcccgtgtcg gagccgcgcg agatatggcc cgcgctggag
    12481 tttcaatacc ggagatcatg caagctggtg gctggaccaa tgtaaatatt gtcatgaact
    12541 atatccgtaa cctggatagt gaaacagggg caatggtgcg cctgctggaa gatggcgatc
    12601 tcgagccatc tgctggagac atgagagctg ccaacctttg gccaagcccg ctcatgatca
    12661 aacgctctaa gaagaacagc ctggccttgt ccctgacggc cgaccagatg gtcagtgcct
    12721 tgttggatgc tgagcccccc atactctatt ccgagtatga tcctaccaga cccttcagtg
    12781 aagcttcgat gatgggctta ctgaccaacc tggcagacag ggagctggtt cacatgatca
    12841 actgggcgaa gagggtgcca ggctttgtgg atttgaccct ccatgatcag gtccaccttc
    12901 tagaatgtgc ctggctagag atcctgatga ttggtctcgt ctggcgctcc atggagcacc
    12961 cagtgaagct actgtttgct cctaacttgc tcttggacag gaaccaggga aaatgtgtag
    13021 agggcatggt ggagatcttc gacatgctgc tggctacatc atctcggttc cgcatgatga
    13081 atctgcaggg agaggagttt gtgtgcctca aatctattat tttgcttaat tctggagtgt
    13141 acacatttct gtccagcacc ctgaagtctc tggaagagaa ggaccatatc caccgagtcc
    13201 tggacaagat cacagacact ttgatccacc tgatggccaa ggcaggcctg accctgcagc
    13261 agcagcacca gcggctggcc cagctcctcc tcatcctctc ccacatcagg cacatgagta
    13321 acaaaggcat ggagcatctg tacagcatga agtgcaagaa cgtggtgccc ctctatgacc
    13381 tgctgctgga ggcggcggac gcccaccgcc tacatgcgcc cactagccgt ggaggggcat
    13441 ccgtggagga gacggaccaa agccacttgg ccactgcggg ctctacttca tcgcattcct
    13501 tgcaaaagta ttacatcacg ggggaggcag agggtttccc tgccacagct tgatgaagat
    13561 ctgagctctt aattaatgat cataatcagc catatcacat ctgtagaggt tttacttgct
    13621 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
    13681 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
    13741 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
    13801 tcttatcatg tctggatccg tttaaactaa ctttaaataa ttggcattat ttaaagttac
    13861 atatggcggc cgccctaagg ctcgacctcg aaattctacc gggtagggga ggcgcttttc
    13921 ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt
    13981 ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt
    14041 ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg
    14101 cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag
    14161 atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag
    14221 ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc
    14281 aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtctt ccggaggccc ggcattctgc
    14341 acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg
    14401 acctgcatcc atctagatct cgagcagctg aagcttacca tgaccgagta caagcccacg
    14461 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
    14521 gccgactacc ccgccacgcg ccacaccgtc gatccagatc gccacatcga gcgggtcacc
    14581 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
    14641 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
    14701 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
    14761 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
    14821 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
    14881 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
    14941 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
    15001 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag
    15061 cgcccgaccg aaaggagcgc acgaccccat gcatcgatga tatcagatcc ccgggatgca
    15121 gaaattgatg atctattaaa caataaagat gtccactaaa atggaagttt ttcctgtcat
    15181 actttgttaa gaagggtgag aacagagtac ctacattttg aatggaagga ttggagctac
    15241 gggggtgggg gtggggtggg attagataaa tgcntgctct ttactgaagg ctctttacta
    15301 ttgctttatg ataatgtttc atagttggat atcataattt aaacaagcaa aaccaaatta
    15361 agggccagct cattcctccc actcatgatc tatagatcta tagatctctc gtgggatcat
    15421 tgtttttctc ttgattccca ctttgtggtt ctaagtactg tggtttccaa atgtgtcagt
    15481 ttcatagcct gaagaacgag atcagcagcc tctgttccac atacacttca ttctcagtat
    15541 tgttttgcca agttctaatt ccatcagaag ctggtcgagt aactttaaat aattggcatt
    15601 atttaaagtt aggcgcgcca taacttcgta tagcatacat tatacgaagt tatgtcgaga
    15661 tatttaaata ttagacccag ctttcttgta caaagtggtt gatatctcta tagtcgcagt
    15721 aggcggggcc aaaaggaatg tgtatctata aaacaatgat tatataaaaa gaggaaaatt
    15781 tctattatct tggggccaaa cttttcatga aataaaagag tgaaattcac ttaacctaaa
    15841 acaagatagg aaagataaat cagtgcataa cctttttact tttaaaataa ttatatctcc
    15901 cacacagaat gtgtgctacc aacagatata agtttaaagc ccttcaccct aaaagctacc
    15961 aaagaaactc ctcccatcag actgagagga aatacactga acgcgtatgg ggatctgtcc
    16021 taatttactg gtctcatttt ggacactagg aacctttaca cactagttcc cgtggagtta
    16081 gatagtcgcc tttcattaac aataaaagat tttcttttct cagctatctg tgtttctgga
    16141 tgaaatttgt catgagacgg aactctctaa caagtatgga ctctcaggct gctgcagcca
    16201 aagtggagtg gaaagacatc agtgtctgct ggcacgcaag aagactgctc cggcctctgt
    16261 cccacccttc cagtttccag aacctgccga gagttgcaaa gcacatgaag aaaacagggc
    16321 agtgttcatg aacaggtaag gaccatcctt gggcatccag tgggcagcca gtgtttctgc
    16381 agtccacatg tgcactccaa agtgtataca agtagggaat atagccagag tatgtctgct
    16441 gaggtctgtg caggggcttt tgttagacac acactgtgag atgcagaaca caccgcatgc
    16501 agactcagcc tctgatccgt cagcctccgg aaacatagct ctttctcacc tttagagatg
    16561 gcgcaacgca attaatgata acttcgtata gcatacatta tacgaagtta tggtctgagc
    16621 tcgccatcag ttcaagagcc aagtgttcat gtacacccac gaaactgata attaagacca
    16681 tgcagtcagc ttctgggata aatgtgcttg tcacaaatgc ttgcgggctt tgatatcacc
    16741 ctctaccctt cctttcacct ttaaaatagt tcattttatt tttgaaacaa ggtcctagag
    16801 gaccaggttg gtcttgaact aagtatgcat taagggtggg tttgagcttc ttatcctcct
    16861 gtctctacct tacaggtgcc aaggggacag gcatatacat acatgcatac atacaacata
    16921 tatttacatg tctatctgtc tatactgata gacatactga gagacagaga cagagaggac
    16981 accttagggt agtcatcgaa ctcttcactg ataaagattt gagctgtctt acgtagaagc
    17041 tagcctggct gattccctga tatgtctgac aggcttactg aaaaagacat ctcagaagga
    17101 atcatagcgt cattgtttgt tttcataaaa gactctggat taacggattg gatctttttt
    17161 ttctcctgag gctgacatgt aggaacgttt tgacagcttt tgaaaaaagc ctgcacattt
    17221 tcccgtaaca gcacagtaga gaaaaagtcc attggaccct gttgcctttg tggttggtta
    17281 ccccattcat taaataaatt ccagagctta gttcagggag gtggcagtcc cttcatctgt
    17341 tacatgtttg caaatctcca agtgttgtcc attccacagg ttcatctatg aagtgtcaag
    17401 gaggaacccc ttcatgtatg ccccagccat tctgtccttg gctgctcagt acgacaaggt
    17461 cgttctggca tgctgcaaag ctgacaacaa ggaggagtgc ttccagacaa aggtggtatc
    17521 tttgtgaagc tatctgaagc taaatggggt ttagccatgg cttgggaaaa cagaattaaa
    17581 ggcagaaatg tgcctcctgt gggaaatacc atcttctttc tcatcctaga tgaaagtcat
    17641 ttttacttga tcttttcaat tatcgatgaa atttcatttt ctatttatag ttagaaacaa
    17701 tactccataa tagtaaggta taactttagg ccgaagtaaa aatgtatttg gatcagcatt
    17761 tactgaatct ttaaagagag cagagataac agcaatgcat gtacccagag gaggtgattt
    17821 agtgcttata agcccattag agtgatagga ttgtccctct ctccttcaga gcagaaaaca
    17881 atgttgatac agagtaacca gcctttcggt aattgacaag tggaaaacat actacaagtt
    17941 caaccagagc caaggaagct ggaaattagt ggggttgcta atgtgtccgc aaaatggagc
    18001 atattcccca cttctggctt gttttatttt gccttttgag gcagactctt gttatatagc
    18061 cccaactggg cccaaactcc taagaattat gacggtagtg caattctttt tttttttttt
    18121 tcttgtcttt tcctttcttt ttaactgagc aggagccaac aggctttacc ccacctggag
    18181 actggacagg tgcagctgtc catgtggtcc cagtatcaaa atgatgacgt attggtttga
    18241 agatgtttaa gagatctcag gcatgcatac ataagaatta atctaaaagt catgaaaata
    18301 gaatgacatg cctaaacgtc ctgagctcta agtttagtag aagctatttt atactttcct
    18361 gtgtcatctg tactgagcgt ttctagttct tagatctgaa gagagaatga atgggttttc
    18421 taaacacctc acttcttctg catctagaga gcatccattg caaaggaatt aagagaagga
    18481 agcatgttaa atgagcatgt atgttcagtg ataagaaaat ttggatcccg aaacctccag
    18541 gcaacgtaag tataagcgtc taaggaggca atgtgatgtg ctcaatgtca atgtcactcc
    18601 tccagagtaa agcctccttt gggaggcaga ctctgattag aatcatgaga tctaaggatc
    18661 agaacagaac tagcagatca gcctgctatt tatgaaagtc tcactggcat ggtattcaag
    18721 gaagagtttt taagtaagtt tttattgttt gttctagaca cttcagtttc tgtccacact
    18781 caaattatac tgccccatta gaccatattt ccccagctct tctacaaagt agatcaagct
    18841 ttatggagag gcatctccga cttgcagcct aataagaaat ccttcttgtt aaccaggctt
    18901 cccaatcaac cccattgtgt caattgttgt ttctcggtaa aatattcccc tgaggcaaca
    18961 actttagaag tcagtgacag gtgggcagag gtccttagtg cctggaagcc taggtattgt
    19021 gcacagccaa gatccaatgc acatttcagc cctgtagttt cttccaaaga ctggtttcac
    19081 ctttcttctg gaagaggtca gacttatgct acaattacct cccaattcta ttagaaactt
    19141 agtcatcaat tcatatggcg ccttaaatat ttgtcttaat agtaaaatag aaatgggagg
    19201 acacaatgac agcaatggca tcatgtatct gttagcaaga aaagccacac acgccacaat
    19261 gattcccaag ttatcagtaa caaagactca atcctttatt caaggaagca gggtctttgc
    19321 acaggcacct cttcccatga attctggcag ctttgtttta tacttccttc tgctgtggtc
    19381 tgatgccttg aagcagaatc tggtagttca ggctggactt gagctcccga ttctcctgcc
    19441 tccacttctg gtttgctgga attgtagccg agtgcttctg tgtctgcaac aaacgctcag
    19501 gatcagggct caacaagaaa gggcgacatt tagcttgagt tcctagaaag tcttcaggaa
    19561 gctacaaatg acttccaaat tacttgagca cccagtcccc cagtctacag agcttgtgcc
    19621 tggtggagca gaagctgacg agacctaaga tgtgtttccg tctcttttaa aagaacacat
    19681 ttctgttctt tcccgctgga ggtaactccc cagatcctct acagacaatg cgatttcatc
    19741 ctgataaaca agataatttt tttttaaatg atcctcttcc agaaccatta ttaagctaag
    19801 tcaaaagtta actgaagcaa attttactga gattcagaag ctggccctgg atgtggctca
    19861 catccacgag gagtgttgcc aaggaaactc gctggagtgt ctgcaggatg gggtaggagt
    19921 ctccctctct ccctttttgt ctcaattaat gtttcactat gcctggcttg gctggggtgc
    19981 agtgaactta cagactttcg aagagtaaaa tttgagctac gtcatgattg ctttggggtc
    20041 tcacctcagg cattagcaat atctgatccc tttcatgaga ctacgagaca ttcagttctt
    20101 atttgactga gagactgagt ctctcccact agctagctag ctgctggttt acagtgaggg
    20161 ccacttgccg ggaaaactta taaactgaca tcaaagaatg tatagtttaa ctggagagat
    20221 aaaacaattg gccttcttga agttatagac tgtcttttgt ttaccattgt ctctacaggg
    20281 caatgtgtgg cacattgaag ggttctgtca tagtttaatg acagacttat aagtaaaaat
    20341 aatgcaagaa actgttgcaa gagagaaaat gtctatttat tactaagcag aggtataaat
//