LOCUS       94189#pL1L2GT1_LF2A_nEGFPOT2A_CreERT2_pAroxbActNeopArox#L3L4_pD223_DTA_T_spec        21673 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2GT1_LF2A_nEGFPOT2A_CreERT2_pAroxbActNeopArox
COMMENT     design_id: 94189
COMMENT     backbone: L3L4_pD223_DTA_T_spec
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            9227..9394
                     /db_xref="ENSEMBL:ENSMUSE00000327111"
                     /rank="2"
                     /note="ENSMUSE00000327111"
     misc_feature    4275..10265
                     /note="5 arm"
     misc_feature    10293..10317
                     /note="B1 Gateway"
     misc_recomb     16443..16474
                     /note="rox"
     misc_feature    16437..16441
                     /note="for bActNeo"
     insert          14357..16436
                     /source="pL1L2_GTIRES_BetactP FLFL MapVerified"
     misc_feature    14660..15180
                     /note="human beta actin promoter"
     misc_feature    15056..15075
                     /note="betact5"
     misc_feature    15206..16191
                     /note="neomycin resistance "
     misc_feature    15375..15394
                     /note="neoPstI_5"
     misc_feature    15712..15730
                     /note="neoSphI_5"
     misc_feature    16005..16024
                     /note="neoBstB1_5"
     misc_feature    16256..16279
                     /note="RAF5"
     misc_feature    16198..16441
                     /note="SV40pA"
     misc_feature    15850..15850
                     /note="G to C conservative mutation to kill RsrII site"
     misc_feature    13392..14336
                     /note="ERT2"
     misc_feature    14360..14602
                     /vntifkey="21"
                     /dnas_title="SV40 pA"
                     /label=SV40 pA
     primer          14417..14440
                     /vntifkey="27"
                     /dnas_title="LRPCR RAF5"
                     /label=LRPCR RAF5
     primer          14543..14561
                     /vntifkey="27"
                     /dnas_title="seq primer NF"
                     /label=seq primer NF
     misc_feature    14603..14610
                     /vntifkey="21"
                     /dnas_title="RE sites"
                     /label=RE sites
     misc_feature    complement(14611..14642)
                     /vntifkey="21"
                     /dnas_title="Rox"
                     /label=Rox
     misc_feature    14643..14650
                     /vntifkey="21"
                     /dnas_title="polylinker"
                     /label=polylinker
     misc_feature    10330..10377
                     /vntifkey="21"
                     /dnas_title="Frt"
                     /label=Frt
                     /note="Frt"
     primer          complement(10342..10365)
                     /vntifkey="27"
                     /dnas_title="FRT3_oligo"
                     /label=FRT3_oligo
                     /note="FRT3"
     intron          10378..11252
                     /vntifkey="21"
                     /dnas_title="mouse En2 intron"
                     /label=mouse En2 intron
                     /note="mouse En2 intron"
     primer          complement(10446..10467)
                     /vntifkey="27"
                     /dnas_title="LRPCR LAR3_EN2"
                     /label=LRPCR LAR3_EN2
                     /note="LRPCR LAR3_EN2"
     misc_feature    11577..12293
                     /note="EGFP"
     misc_feature    11487..11576
                     /note="3X NLS"
     misc_feature    12357..13382
                     /note="Cre"
     misc_feature    12303..12356
                     /note="self-cleaving' 2A peptide"
     misc_feature    12353..12353
                     /note=" A to G to kill RsRII site"
     exon            11253..11306
                     /vntifkey="61"
                     /dnas_title="mouse En2 exon"
                     /label=mouse En2 exon
                     /note="mouse En2 exon"
     primer          11153..11174
                     /vntifkey="27"
                     /dnas_title="seq primer FCHK2"
                     /label=seq primer FCHK2
                     /note="seq primer FCHK2"
     misc_feature    11307..11429
                     /note="1Dleader"
     misc_feature    11430..11481
                     /note="F2A"
     misc_feature    11302..11307
                     /note="FCHK QC suffix region"
     misc_feature    11268..11283
                     /note="FCHK QC prefix region"
     misc_feature    11284..11301
                     /note="FCHK QC critical region"
     misc_feature    16487..16507
                     /note="B2 Gateway"
     misc_feature    10266..16537
                     /note="Synthetic Cassette"
     exon            16889..17148
                     /db_xref="ENSEMBL:ENSMUSE00000161041"
                     /type="targeted"
                     /rank="3"
                     /note="target exon 1 ENSMUSE00000161041"
     misc_feature    16538..17367
                     /note="Critical Region"
     misc_feature    17391..17424
                     /note="Downstream LoxP"
     misc_feature    17368..17447
                     /note="synthetic loxP region"
     exon            18577..18657
                     /db_xref="ENSEMBL:ENSMUSE00000161044"
                     /rank="4"
                     /note="ENSMUSE00000161044"
     misc_feature    17448..21673
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggagcacaa attatgaaag tgaaattagt ctccaaaagg gttgtttaca
     4321 aacttttctt atgaaaggca agacagaaag catcccaggc ttgggatctg gatgtggctt
     4381 ctggtgcata tgactttgta tgactttaat ggcacttgaa aatatagatg acatgcttga
     4441 cagactgacc acacactgtc aaccccagca cagaacttgt cttaagagtt atagtttgct
     4501 aactactgat atggatgaga taaaatcagg tcttttgcaa tatgacttgg attctagact
     4561 ttccctcatt atatggagat aattggttta tctttatact aatgtaaatt aggacaagca
     4621 agatggctca gtgggtaaaa gtgtttgcca tgcaaatttg aggacctgag tttgatcccc
     4681 agaacacaaa tggtaggaga aaaatgactg tgaattgtcc tatgacctct gtgtgtactg
     4741 tggtagatcc ttgtctgcac tcatctcatt tcctttctcc ctctctccct ccactctctc
     4801 tttgtcacac acattgataa taatgaatat ttttaaataa taataaaaca tagagtatca
     4861 gaatgagact agaatgctgt cgatggtgag tgtgagtagt attgttcatc cctgttgtct
     4921 gtcctagcgc tgcagggtct gccaagcatt ctgaataaaa cacgtgtatg ttaaaaaaaa
     4981 atcttgacat tcaagaaaat ttataactta agtgaggaga aagacagtta aacctctcct
     5041 gaggaagcga cttgtgggac tttgtgccag atgagtgata gagataagtt ctgctctgca
     5101 aaagcagaag gaagggccct ttcataacga tggtcagaga ccagacagtc tgactacttt
     5161 gtatttcctt accttctttg ttcctcagaa caaccaggag gcagggccta ctttgatttc
     5221 attttactgg agaagtttaa aagttgtcag ttgccttgga gcctccaagc tagaactcag
     5281 aaaagaagca cctggaacat ctacctacaa gacttggcct ctttgtctct gggaaggtct
     5341 gccttcaaat tgggtagtgc agtggctcac ctctcaggga tgtgacttca gcacagatat
     5401 agtcagaaat gttgtactag tgaggggctg gctgaggagg ggatcagaaa ataagaacta
     5461 aaactagcct ggtgtggtgg tgtaccccag gaatctcagc actcaggagt cctaggcagg
     5521 gcaaacagga gtgcaacacc acccttagct aaagtggctt gcataaaatg tcatttagcc
     5581 tgggctacat ggcacctctc tgaaagaaaa acaaaacaaa acaactacaa aaaaaaaacc
     5641 caagctctat aaagatatat gaaaaaatta gaccaagaat gtatggtaaa ttggtaattt
     5701 aaataataac ttactgaatc ttttagaata tgtaaccttt ttatcaaaac tggacaaaac
     5761 tggaatatat atttcatatg taaatatata taataaaaac tggaataaaa aatataaatc
     5821 tccagtttac taatattaag taaatttttt caaaatgtat tacacatgcc tttgtttctt
     5881 cagagtacct acccaaagga ctttaacttt catgaagtgt ctgtttttca ttttgaaatt
     5941 tcagtttctt agttttcagc tgttgcttct ccctatatgt ttctaatttt caactttgac
     6001 tagaatatca atataaccag gcttatatta atgctgtatt acaatgtgaa cacagtatta
     6061 atattgtatt accaaagtaa gtacctgtaa ggtcagtgtc tgaatttcac tacaaaaatt
     6121 cttggtgtta catctgttgg tgtcctaaaa tcctcattta ataacttgcc catagcttgt
     6181 ttcctgtctt gttccaagtt tgttaagtga agattgacag gaagaagtta gaaagatgat
     6241 ttaattctga gttaatctat ctttcctgag gcatgagaga ggggcttttc ctcatgtcca
     6301 tgtggaatag aggaaaggtt tccttctttg attttcagca tctgaaaggc attcagagag
     6361 tttaggcttc tcttcttccc cagagatttg gagaaagaag gccagtgttg gatggtttaa
     6421 ggtagtggat ataggcagaa agagagaaca aaaagacatt ggtagcctta gtagaacctt
     6481 tttaaaaatc tattagattt ataatcttta taatctatta gctctgcaaa agagccatag
     6541 aggctaagta aggcccaggg atgcaccata gaagacctat gctgcagttt gtaagtcagg
     6601 tgtgactcct gacctaaggc gatggtagtc cctcatggta agctagttct tatatagctc
     6661 ttcatgtaac cttgcttttc tgaccctctt ctttgttctg ttctggtagc ctcacaagtg
     6721 ttgctgctta ctctaggctt ggagctatac atctgtcttg ggtcctcttt tcatttgcaa
     6781 tctggccaaa gagtgggaga gatgaggctg ctacagctgg gagcccatcc ccaatgggtc
     6841 catagctagc ttggatctgg agttgattca ctgttctgga acaggcacct ctgtctttag
     6901 aactagcagc gggtctctct tcacatctgg aaaagttcaa aattgtttgt ctgggtgaag
     6961 gaggcccctt gggggagggg ttcaaaagca tgacatcatc tcagccaggc ctggtttcct
     7021 ttggggttgg gccaacctag accagatggg attcccagga attgacgtgt ttgagtagag
     7081 caaggagggt gtgggcagaa agaaggattt caaagggaaa ttcaagggat atcttagaat
     7141 ggtaaattga aaatatgatt cctttaattc agtgtctaaa tgctaccacc actttccctt
     7201 ataataaatt aatgtctagg gtcagattgg cttctgtatc tagactaaat aggaatagac
     7261 atatatccta ctgtccacaa aggctttgag atccttaaca actacctagt cagcaagacc
     7321 tacactcttt gctgcttgcc caaatgatct atattcttcc cctgaccaaa aacttggtga
     7381 catagatttt tcatttttag aatgaagata gtaatattac ttttttccct atttacctga
     7441 catataataa aacatgattt ctgtggtcaa tattaataca gcctgcttgc tacttgttgg
     7501 gaagatggca aagatgggag agggaaccct ggctagcacc tatggaaaac attcttggcc
     7561 ttgggttgac tagaagttaa aattctaact tccctctaga tgttgatggc aatagcaatc
     7621 agtctacatt gttgaccttg ggaaatggaa aaaatacaga gttagatttc aatcaggaaa
     7681 ctttagagat gcacttcagg tatccttaca aatattatta cagttgtcag ctatggagtc
     7741 tgagttggtt tctgaacaat tcacaaagag gcccacatcc tcttgtatgc ttgaactcca
     7801 catttgtagt tgaggccaac ttgtaaacta agcagctcat ccccataccg taaccataca
     7861 ccttctcagt cattgagaaa actaactttg aatctggaac tttaacactt ttggatgtca
     7921 gtcttctgtg atttgtcagg acaagataga ccttggcccc agcttgcggt ccttcccttc
     7981 ttcctgccca caactctggc cccagtctac ttctttcctc ttctttctac taatgagtct
     8041 ggccccagcc cacttccctt accttcttcc ttcacatgac tcaggccctc agtgtggagg
     8101 atcttgcatg tacatatagg acactagtat acccatatgc taacccttct cccttcaaac
     8161 agttactcct ggaccttgga agtacacatt ttggtactga agttcattcc tagaaggatg
     8221 agccatcaca ggttaatgca ggtctgaatg tgagcaaagg gccattggag caaagtgtat
     8281 gaagacttgt agtctccaaa gcgtgatcta gaagggaggg tcatcttggt ttgaccatac
     8341 aggaagtaaa acaggctttg ggacacataa gatataaagt caaggttctc cttccaagtc
     8401 taaagatata tagaagaatg tgcaagggca atcataaggc tattcagaga atgcatagat
     8461 caaaaatcat tctaatttag tagtttgatt tcctgacaat tcatgtaaaa atagacaaac
     8521 aacctacatt taaggattaa actgaggtgt gcatgcttga aaatgtgtgg tttctggcaa
     8581 agctctctgt cctccctcta tgagacagaa tgagaaacca gtcccatcaa gttaaattct
     8641 agcttagaac ctcctgaaag aaatctccag gttgctcact gcagttgagt tcttcattta
     8701 aagaaaagga taatgggttt gaaggtcctg aagtttctaa atgagcaaga ctgagaatga
     8761 aaaattatgc aaggggattt tcatagagaa attatgaatc tagattattt ctaaaaatta
     8821 attgaacttg agcaaaaaag aaaagtgtgt aaaatggagc taggaactta gcctctcaca
     8881 ttttcaatcc ttgttatgtt tacataggta atattttgta tcttggtatt ttgctaaaca
     8941 cattacaagc aatgacttat atttctgtgt tgctacagtt tttaattatt tgtgacattc
     9001 ctgtgtttat aaatgctcag tatatttcat tagggaaagc ctagtaatag gaaactagtt
     9061 tgatgtctag gaatacatta ttaatgtttc tccattatca tgggcaacaa ggatgttcca
     9121 gtcagattca cctaaagcca tgaagttgaa tcaggtgttg ttataaggtt ccaggaaggt
     9181 tgagttctaa cttgtcctta tttccctgta acctcttggt ttacagatct aaataacttg
     9241 acttctgcta ccacggagac ttctacacaa ggaatatccc catcagttcc taccaatgag
     9301 tctgttgagg aaaatatcac atctagcatc cctggaagta ccagccacta cttgatctat
     9361 caggacagca gtaagaccac accagccatc tcaggtaact agaagtagtc aactagtaca
     9421 gcctgttccc ccaccacaga tgaggtcata taaaccaatg attcaagaaa tggtattctt
     9481 ttttttatta ttagatgttt tcttcattta catttcaaat gctatcccct ttcctagttt
     9541 cccctccgaa tatcccctat accctgtccc ctccccctgc tgcccaaccc acccactcct
     9601 acttcctggc cctggcattc ccctatactg gggcatagaa tcttcacaag accaagggcc
     9661 tctcatccca ttgatggccg actaggccat cttctgctac atatgcagct agagacacat
     9721 gctctggggg tactggttag ttcatattgt tgttcctcct ataatgctgc agaccccttc
     9781 agttccttgg gtactttctc tagctccttc actggggacc ctgtgttcca tccaatagat
     9841 gactgtgagc atccacttct gtatttgcca ggcactggca tagcctcaca ggagacagct
     9901 atatcagggt cctgtcagca aaatcttgtt ggtatatgca atagtgtctg ggtttggtgg
     9961 ttgtttatgg gatggatccc tgggtggagc agtccctgga tggtcctttc ttccgtctca
    10021 gctccaaact ttgcctctgt aactccttcc atggatattt tgttccccta tctgagaagg
    10081 aacgaattat ccacactttg gtcttccttc ttcttgagta agaaatggta ttctaaataa
    10141 gttctaaata gctttctctg tgttggaaac atttgtaggc catacatgac aagtgggtcc
    10201 agaataggac tgattctcat agtcatgcta agttcagctg gtataagtaa ggatcagttt
    10261 ggattaaggc gcataacgat accacgatat caacaagttt gtacaaaaaa gcaggctggc
    10321 gccggaaccg aagttcctat tccgaagttc ctattctcta gaaagtatag gaacttcgaa
    10381 ccctttccca caccaccctc cacacttgcc ccaaacactg ccaactatgt aggaggaagg
    10441 ggttgggact aacagaagaa cccgttgtgg ggaagctgtt gggagggtca ctttatgttc
    10501 ttgcccaagg tcagttgggt ggcctgcttc tgatgaggtg gtcccaaggt ctggggtaga
    10561 aggtgagagg gacaggccac caaggtcagc cccccccccc tatcccatag gagccaggtc
    10621 cctctcctgg acaggaagac tgaaggggag atgccagaga ctcagtgaag cctggggtac
    10681 cctattggag tccttcaagg aaacaaactt ggcctcacca ggcctcagcc ttggctcctc
    10741 ctgggaactc tactgccctt gggatcccct tgtagttgtg ggttacatag gaagggggac
    10801 gggattcccc ttgactggct agcctactct tttcttcagt cttctccatc tcctctcacc
    10861 tgtctctcga ccctttccct aggatagact tggaaaaaga taaggggaga aaacaaatgc
    10921 aaacgaggcc agaaagattt tggctgggca ttccttccgc tagcttttat tgggatcccc
    10981 tagtttgtga taggcctttt agctacatct gccaatccat ctcattttca cacacacaca
    11041 caccactttc cttctggtca gtgggcacat gtccagcctc aagtttatat caccaccccc
    11101 aatgcccaac acttgtatgg ccttgggcgg gtcatccccc cccccacccc cagtatctgc
    11161 aacctcaagc tagcttgggt gcgttggttg tggataagta gctagactcc agcaaccagt
    11221 aacctctgcc ctttctcctc catgacaacc aggctcccag gtcccgaaaa ccaaagaaga
    11281 agaacgcaga tcgcagatcg cagatctcta gagtcaccga gttgctttac cggatgaaga
    11341 gggccgaaac atactgtcca aggcccttgc tggcaatcca cccaactgaa gccagacaca
    11401 aacagaaaat tgtggcaccg gtgaaacaga ctttgaattt tgaccttctc aagttggcgg
    11461 gagacgtcga gtccaacccc ggaccggtgc atgtggaccc caagaaaaag cggaaggtgg
    11521 accctaaaaa gaaacgcaaa gtggatccta agaaaaaaag aaaggtgcac gtgtccatgg
    11581 tgtccaaggg cgaggaactg ttcaccggcg tggtgcccat cctggtggaa ctggatggcg
    11641 acgtgaacgg ccacaagttc agcgtgtccg gcgagggcga aggcgacgcc acatacggaa
    11701 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccttgg cctaccctcg
    11761 tgaccacact gacctacggc gtgcagtgct tcagcagata ccccgaccat atgaagcagc
    11821 acgacttctt caagagcgcc atgcccgagg gctacgtgca ggaaagaacc atcttcttta
    11881 aggacgacgg caactacaag accagggccg aagtgaagtt cgagggcgac accctcgtga
    11941 acagaatcga gctgaagggc atcgacttca aagaggacgg caacatcctg ggccacaagc
    12001 tggagtacaa ctacaacagc cacaacgtgt acatcatggc cgacaagcag aaaaacggca
    12061 tcaaagtgaa cttcaagatc cggcacaaca tcgaggacgg ctccgtgcag ctggccgacc
    12121 actaccagca gaacacccct atcggcgacg gccctgtgct gctgcctgac aaccactacc
    12181 tgagcaccca gagcgccctg agcaaggacc ccaacgagaa gagggaccac atggtgctgc
    12241 tggaattcgt gaccgccgct ggcatcaccc tgggcatgga cgagctgtac aagcctgcag
    12301 gcgagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc gggccgtcca
    12361 atttactgac cgtacaccaa aatttgcctg cattaccggt cgatgcaacg agtgatgagg
    12421 ttcgcaagaa cctgatggac atgttcaggg atcgccaggc gttttctgag catacctgga
    12481 aaatgcttct gtccgtttgc cggtcgtggg cggcatggtg caagttgaat aaccggaaat
    12541 ggtttcccgc agaacctgaa gatgttcgcg attatcttct atatcttcag gcgcgcggtc
    12601 tggcagtaaa aactatccag caacatttgg gccagctaaa catgcttcat cgtcggtctg
    12661 ggctgccacg accaagtgac agcaatgctg tttcactggt tatgcggcgg atccgaaaag
    12721 aaaacgttga tgccggtgaa cgtgcaaaac aggctctagc gttcgaacgc actgatttcg
    12781 accaggttcg ttcactcatg gaaaatagcg accgctgcca ggatataagg aatctggcat
    12841 ttctggggat tgcttataac accctgttac gtatagccga aattgccagg atcagggtta
    12901 aagatatctc acgtactgac ggtgggagaa tgttaatcca tattggcaga acgaaaacgc
    12961 tggttagcac cgcaggtgta gagaaggcac ttagcctggg ggtaactaaa ctggtcgagc
    13021 gatggatttc cgtctctggt gtagctgatg atccgaataa ctacctgttt tgccgggtca
    13081 gaaaaaatgg tgttgccgcg ccatctgcca ccagccagct atcaactcgc gccctggaag
    13141 ggatttttga agcaactcat cgattgattt acggcgctaa ggatgactct ggtcagagat
    13201 acctggcctg gtctggacac agtgcccgtg tcggagccgc gcgagatatg gcccgcgctg
    13261 gagtttcaat accggagatc atgcaagctg gtggctggac caatgtaaat attgtcatga
    13321 actatatccg taacctggat agtgaaacag gggcaatggt gcgcctgctg gaagatggcg
    13381 atctcgagcc atctgctgga gacatgagag ctgccaacct ttggccaagc ccgctcatga
    13441 tcaaacgctc taagaagaac agcctggcct tgtccctgac ggccgaccag atggtcagtg
    13501 ccttgttgga tgctgagccc cccatactct attccgagta tgatcctacc agacccttca
    13561 gtgaagcttc gatgatgggc ttactgacca acctggcaga cagggagctg gttcacatga
    13621 tcaactgggc gaagagggtg ccaggctttg tggatttgac cctccatgat caggtccacc
    13681 ttctagaatg tgcctggcta gagatcctga tgattggtct cgtctggcgc tccatggagc
    13741 acccagtgaa gctactgttt gctcctaact tgctcttgga caggaaccag ggaaaatgtg
    13801 tagagggcat ggtggagatc ttcgacatgc tgctggctac atcatctcgg ttccgcatga
    13861 tgaatctgca gggagaggag tttgtgtgcc tcaaatctat tattttgctt aattctggag
    13921 tgtacacatt tctgtccagc accctgaagt ctctggaaga gaaggaccat atccaccgag
    13981 tcctggacaa gatcacagac actttgatcc acctgatggc caaggcaggc ctgaccctgc
    14041 agcagcagca ccagcggctg gcccagctcc tcctcatcct ctcccacatc aggcacatga
    14101 gtaacaaagg catggagcat ctgtacagca tgaagtgcaa gaacgtggtg cccctctatg
    14161 acctgctgct ggaggcggcg gacgcccacc gcctacatgc gcccactagc cgtggagggg
    14221 catccgtgga ggagacggac caaagccact tggccactgc gggctctact tcatcgcatt
    14281 ccttgcaaaa gtattacatc acgggggagg cagagggttt ccctgccaca gcttgatgaa
    14341 gatctgagct cttaattaat gatcataatc agccatatca catctgtaga ggttttactt
    14401 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
    14461 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
    14521 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
    14581 gtatcttatc atgtctggat ccgtttaaac taactttaaa taattggcat tatttaaagt
    14641 tacatatggc ggccaattcg ccttctgcag gagcgtacag aacccagggc cctggcaccc
    14701 gtgcagaccc tggcccaccc cacctgggcg ctcagtgccc aagagatgtc cacacctagg
    14761 atgtcccgcg gtgggtgggg ggcccgagag acgggcaggc cgggggcagg cctggccatg
    14821 cggggccgaa ccgggcactg cccagcgtgg gcgcgggggc cacggcgcgc gcccccagcc
    14881 cccgggccca gcaccccaag gcggccaacg ccaaaactct ccctcctcct cttcctcaat
    14941 ctcgctctcg ctcttttttt ttttcgcaaa aggaggggag agggggtaaa aaaatgctgc
    15001 actgtgcggc gaagccggtg agtgagcggc gcggggccaa tcagcgtgcg ccgttccgaa
    15061 agttgccttt tatggctcga gcggccgcgg cggcgcccta taaaacccag cggcgcgacg
    15121 cgccaccacc gccgagaccg cgtccgcccc gcgagcacag agcctcgcct ttgccgatcc
    15181 tctagagtcg agatccgccg ccaccatgat tgaacaagat ggattgcacg caggttctcc
    15241 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
    15301 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
    15361 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
    15421 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
    15481 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
    15541 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
    15601 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
    15661 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
    15721 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
    15781 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
    15841 gggtgtggcc gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
    15901 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
    15961 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
    16021 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
    16081 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
    16141 ggggatctca tgctggagtt cttcgcccac cccccggatc taagctctag ataagtaatg
    16201 atcataatca gccatatcac atctgtagag gttttacttg ctttaaaaaa cctcccacac
    16261 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
    16321 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
    16381 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
    16441 cctaacttta aataatgcca attatttaaa gttaggaatt aattcaccca gctttcttgt
    16501 acaaagtggt tgatatctct atagtcgcag taggcggagg ataaaatttt cagtttcata
    16561 attagagtgc accttggcta cattctagat cttgcatgga caatctgata cctgcaggtc
    16621 tcatacatct caggttagct acaaataagg tccaacatat ttgtaatgat aacatcatga
    16681 tacaatgtta ataaaagggt ggacacccct gctatatatt atagctatgg ttctaacagc
    16741 tggcttgaat tgagttgggc caggattaag atataaagta actgtaacct caccaaattc
    16801 ctgtttctgt gcagaaatct acaaagtgag attgtgtgtt tctgagtgca ttgtgaagat
    16861 gcatatatac tgttttggtg tattccagag actatggtca actttacagt tacctctggg
    16921 atcccttcag gctctggaac tccacacact ttttcacaac cacagacttc cccaactggc
    16981 atactgccta ctacttcaga cagtatttcc acttcagaga tgacctggaa gtccagcctg
    17041 ccatctataa atgtttctga ttattcgcct aataatagca gctttgagat gacatcaccc
    17101 accgagccat atgcttacac atcatcttct gctccgagtg ccattaaggt gggtgagttg
    17161 ggccccaaac agcagattat gcctcactgc tcataaaagc atgagattta ggtatcttgg
    17221 agtcaacatg gaatatgtat tttatattgc tagaaaggct agtcaggagg aagatgggga
    17281 ggagagggtg aagtccagtg agaagtctga tctgtgcttt aggttttgca ctttggggag
    17341 attcttagag atccttctag ctctctagag atggcgcaac gcaattaatg ataacttcgt
    17401 atagcataca ttatacgaag ttatggtctg agctcgccat cagttcaggg aatatttgta
    17461 tctgtcccct gtctactggg gttgcctttc ttaaaatatt tagggcaaag tatttctcat
    17521 atatgtaagg gcttagtagc tgtcccaggc cagaatggca gtgaggatgt catctaatga
    17581 attcagaact ggacacataa gggtggtgag ttcagtctga ccttcttggc attttagcct
    17641 gtggcaatca ccagctttca atctgacaga cagaaattag agcatgtagc ctacagacag
    17701 ccatgaggga cctagctttt gtttttaaag agatagcaga taaatggaaa gactcttacg
    17761 ggccaagaga aaaacgggtg tcaacaacag ctgcctggct actccgttgt cagtgtgtaa
    17821 ttgactgaag agaaaaacaa aacaatttct tcttttgccc tttaagcatc actaggggtt
    17881 cccaagataa gcaaatggcc tcttgcttat ggtctagatt tgttaacaaa aggcttgatt
    17941 ctctcatagg agtagtttat aagatgggaa caatgtaact acactcctcc tacccttatc
    18001 tgaaaatctt tagtccagcc tttttatttt ggaatacata atttgatttt tgaataaata
    18061 atgagatatc ttgaggataa gacccaaaat caaaacttgt ctgtgcttct tatataccat
    18121 aagcacataa tttaagggtc atcttatatc atacttttca ataattttat acattcaaaa
    18181 atctgtagta tatatgcccc acttacgtca ccacattagg gatcacaaag atttagattt
    18241 tcaaacactt tatattctga attttttgat taagggtgct caatttttat ttcaaatctc
    18301 actgggcatg tcaacaacaa ctaaaggcag gacaattatg agttgaggcc agcaggggtt
    18361 acattgctac attgtgaatt ctggtctacc ctgatctata gaaaaaaaaa aaaaaaagaa
    18421 gtaactaaat aaatcacaga aatctctagg cctctgacaa gctttaaatt tcctttttcc
    18481 taatcttttc aagagaatac catgacagaa atgaggtgag gaagaagtga ccagcaagga
    18541 tacttgcatg ttaactttgt ttttctttta ctatagggag aaatcaaatg ctctggaatc
    18601 cgagaagtga ggttggccca gggtatctgc ctggaactaa gtgaagcatc tagttgtgta
    18661 agttggcctt ggtcttcctc ccacacaccc caacccctct gaaaccctga agctaccctg
    18721 aattggcctc ctctgaaaaa ggaatctggg acttaacaga gtcaggctat ttctgatatt
    18781 gggtttcggc agtcagaggc cccatctagt gagcaagaag ggcaaaaaca atttaaattg
    18841 tgttgttatt tgaagtcaga tttaaccttg cttttatgtt gagaaattcc ttgtcattgc
    18901 ccagatatga accattcaac taaactgtaa ctcaaaagac ttacatgtaa ctctttagtg
    18961 cacactgaat ttatttttgt gtttatatta agtttgtata tgatctggta cttcagaggt
    19021 attttatttg tccattttaa tctgctgtgt tttcatatta cagtgctttt aatatccaac
    19081 aagccagatc tttcctaatt agaccacaga aggatgattc acaactcttt ttttaaaaaa
    19141 atcttaaaaa gtcacacaaa tttgaataag aacttgtctg aaatccttga gttttttcta
    19201 gtcttatttt gtatctttta tcattttttt aatttaaaat gtttccctat ttattcccac
    19261 tcttcttcaa catatttttt ttcttttggg taggaatgga acccaagacc ttgtacatac
    19321 taggcaaatg ttttgctgct gaggtatatc cccaacattg tttactataa gtttgatgga
    19381 ttttaaacaa ggatatccta acatatattt gctatcatgt agatattaga aaattattgg
    19441 agtctaatat ttatctttag tttctgtctt tcttgaatct gttaattcta ggagcttctt
    19501 ggttggttct ttttcatttt taaagtgaac taccaaggta tctactatga tgtgttattt
    19561 ttctcatttt ttttaaaaca atagtgattc ttattttatt tccttgtcag actgcattgg
    19621 ttagaatgta catggcatgg tgtgaatttt gtgtgttaca gtaaatcctg tttcctgaca
    19681 gtaactaggc cctggagaaa gaatggatgt gaaagaaaaa gtgctgtttt cttttacagt
    19741 aaaatatttt cttacaaatt gggaatgcag tgaagggaaa cttgtagcta acagctggct
    19801 gcatggttta gacagacttt aggctgtagg gaacatgcct tatttatttt ggctactcaa
    19861 ataactggtt tcttacctac agctatcagg tgggtacctc tgacttacaa atctttttaa
    19921 actacatatt aaaaggatcc tttaacttaa gagcatttaa aagtgacaga aatcatagat
    19981 acaagttttt agaataactc agtgcagttt tcaaaaacat ttagggtaaa cataaatagg
    20041 caaagtacaa gcaacagtta atgtgtgcct gatgccaatt tttaacctta ctgattaaag
    20101 ctaaagacaa aataatccca tcagttttta ttttgcctat cttaatggag tattctttca
    20161 tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtatgtatg tgtgtgtgtg
    20221 tttgtgtgtg tgtgtgtgtg tgtgtgtgtt attacataga tctgtgccag aatgctagtt
    20281 ctttgtgctc agtatttcct atccttctgc tgttctcagg atcacatatt gtcatcatct
    20341 aagataagtg cttctctgga aagaactgtt ctttctacct gtggtctatg cagtcagtct
    20401 gctccattcc tgaccagaga aaactctgtg tgttcctttg agattggtgg aggatctagg
    20461 attagggtct ggatggagca gccccatcca acctccacct gagccaataa caggacttcc
    20521 acacttcaca aggtctacca ctaccgagca catgtgaagc attgtcattc tctggagttc
    20581 ccatcactcc agagggatgt tctcatccca tttcttcccc agagctcttc atcccaaggc
    20641 ccaagcagac ctcctggggg aatttttctc tggatccaga atttctagcc atggatccca
    20701 atgtttcaag attcccttct tagtcctgct agtctaatag tctattgcaa agggtacagt
    20761 gcctcctagc tgccataggt agtgtgtctg cttgctgtca gggggctttt aagtactggc
    20821 tttcctagtg cagaggttct caacatgtgg gtcatgaccc ctttgggggt caaaggatgc
    20881 tttcatgggg gttgcctgag accattggaa aacacagata tttacattag ggttcagaac
    20941 agtagcaaaa ttacagttat gaagtagtaa caaaataatt ttatgattgg gggttaccaa
    21001 aacatgaggg actgtgctaa aggaccacag cattgagaag attaagaacc actatactag
    21061 ttggagactc aggccctacc cacaccccaa attaggaaca taaaggattt ggtttttccc
    21121 tatgaacaca tcctgcagaa ctcatttgat actgatacct aactagtaca agtttcaaca
    21181 taactgtgta tgtggtcctc agccttccca gcccccattt tgcaagatgc atgatgagca
    21241 acaaggtgct agctctccct gtgtagacca tttagatctt ttagtataaa cattcagtta
    21301 cagtgactcc ccactaactc tcctgttctg catcctttgc ttctgagcat cttatacatg
    21361 ctaaggaata gaatgttttc aagcatgttg cctaatctca aagtccttgt tagtagagca
    21421 tcatgtctcc ccaggtctta tttccattct ttccacttca ctatctgctt aatttagtgt
    21481 ttgtgatcaa gattctcaag atcctttcta aaatataaga ttactttatt ttcttatatc
    21541 gtagtcatag gtacagggga attcaccaac atttttaatt gtagtaaaat acccacaaca
    21601 tgaaaagtac taccattaca atttctcatt aaaactgagt aatttgaaca tcaacactac
    21661 atataccttg aag
//