LOCUS       211393#pL1L2_GT1_LF2A_nEGFPO_T2A_CreERT_puro#L3L4_pD223_DTA_T_spec        23331 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT1_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 211393
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000098669
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            9355..9714
                     /db_xref="ENSEMBL:ENSMUSE00001027988"
                     /rank="2"
                     /note="ENSMUSE00001027988"
     exon            8433..8587
                     /db_xref="ENSEMBL:ENSMUSE00000475696"
                     /rank="1"
                     /note="ENSMUSE00000475696"
     misc_feature    4275..10727
                     /note="5 arm"
     misc_feature    10755..10779
                     /note="B1 Gateway"
     misc_feature    10792..10839
                     /note="Frt"
     misc_feature    10840..11339
                     /note="mouse En2 intron"
     primer          complement(10908..10929)
                     /note="LRPCR LAR3_EN2"
     primer          complement(10804..10827)
                     /note="FRT3"
     primer          15005..15023
                     /note="seq primer NF"
     misc_feature    15065..15072
                     /note="RE sites"
     misc_feature    16865..16898
                     /note="loxP"
     misc_feature    complement(15073..15104)
                     /note="Rox"
     misc_feature    complement(16825..16856)
                     /note="Rox"
     misc_feature    15105..15126
                     /note="polylinker"
     CDS             15685..16281
                     /note="puromycin N-acetyl transferase"
     misc_feature    15800..15805
                     /note="Mutated RsrII site"
     intron          11340..11714
                     /note="mouse En2 intron"
     misc_feature    11712..11714
                     /note="Splice Acceptor"
     exon            11715..11768
                     /note="mouse En2 exon"
     primer          11615..11636
                     /note="seq primer FCHK2"
     misc_feature    11769..11891
                     /note="1Dleader"
     misc_feature    11892..11943
                     /note="F2A"
     misc_feature    12039..12755
                     /note="EGFP"
     misc_feature    11949..12038
                     /note="3X NLS"
     misc_feature    14821..15059
                     /note="SV40 polyadenylation site"
     misc_feature    13854..14798
                     /note="ERT2"
     promoter        15137..15648
                     /note="PGK promoter"
     misc_feature    16392..16824
                     /note="PGK pA"
     primer          14879..14902
                     /note="LRPCR RAF5"
     misc_feature    12765..12818
                     /note="T2A"
     misc_feature    12815..12815
                     /note="A to G to kill RsRII site"
     misc_feature    12819..13844
                     /note="Cre"
     misc_feature    11730..11769
                     /note="FCHK lf2a_lacz_gt1"
     misc_feature    11730..11745
                     /note="FCHK QC prefix region"
     misc_feature    11746..11763
                     /note="FCHK QC critical region"
     misc_feature    11764..11769
                     /note="FCHK QC suffix region"
     misc_feature    16921..16941
                     /note="B2 Gateway"
     misc_feature    10728..16971
                     /note="Synthetic Cassette"
     exon            20052..20327
                     /db_xref="ENSEMBL:ENSMUSE00000965665"
                     /type="targeted"
                     /rank="5"
                     /note="target exon 1 ENSMUSE00000965665"
     exon            18073..18327
                     /db_xref="ENSEMBL:ENSMUSE00001033065"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 2 ENSMUSE00001033065"
     exon            17321..17605
                     /db_xref="ENSEMBL:ENSMUSE00001040600"
                     /type="targeted"
                     /rank="3"
                     /note="target exon 3 ENSMUSE00001040600"
     misc_feature    16972..20607
                     /note="Critical Region"
     misc_feature    20631..20664
                     /note="Downstream LoxP"
     misc_feature    20608..20687
                     /note="synthetic loxP region"
     misc_feature    20688..23331
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggaaatatt taaacaatat gcaataacat tatgcctaca gagagagtgt
     4321 gggggggaag agaaacacac actcattgct ggtgggtgtg caaattaata gccattatac
     4381 aaagctgtgt ggaagtctct taaaaaatag caataccata taacgcagct atttcactgg
     4441 acatataccc agagaacctg taccctacca ttgagatatc tgcactccca tgattacagc
     4501 tgctccatcc catatagcaa ggaagtagaa ccaacctaga tctccatcaa tatatgaatg
     4561 cacaatacaa gtctaccaca cactcacaca tacacacaca cacacacaca cacacactca
     4621 cacatacaca cacacacaca cacacacaca cacactgtga agtattgttc gtctctacag
     4681 aaagatgaat cacaaagttt acagggatgt ggatagactt agaatatatc atgttaaatg
     4741 agatcatttg atctcaggaa gaaaacccca tatgtccttt cttgtttgtg gatcctagct
     4801 ctaatgtctg catgtacaca tgtaattaca tatataacat taggaaggag aacatgaagg
     4861 ggtttggtct ggtttggttt ggtttggttt ggtttggttt tcaagacagg gtttctctgt
     4921 gtagccctgg ctgtcctgga actcactctg tagaccaggc tggcctgaaa ctcacagaga
     4981 tcctccttcc tctgcctccc gagtgttgga ggtgtgcgcc accgcctggc aatggtgtgg
     5041 tagttctaag gagacaaagt gctccctcat gggctcacac atatgatgta cctggtgtgg
     5101 gccctttgga tgctctctcg catgtaaccg gttttgacgt aaaacaggcc cttagcaagc
     5161 agtgtccacg tgggactcca tgcggggctg ctgccttact gggaggcctg gatctgaagt
     5221 tctgtctttc atcctttgaa tattcctgta cagtgtagtt cttgtaagta ccaacaatta
     5281 gggcccatag ttctttaaac aagaatttca cctataaaaa ctatagagag tgaagacact
     5341 cataagtatg cttaaataat cataattatg aatttataaa acaaattcca aggaatgctg
     5401 actcactgct actgcatctc aaatactaac acaccaggaa ttacacacac taagaagctt
     5461 cctttttgtg gtttttaatg ttacaattct ttttttaata tatgtatata atttttatta
     5521 ggtattttcc tcatttacat ttccaatgct atcccaaaag tcccccatac catccccccg
     5581 actcccactt tttggccctg gcgttcccct gtactggagc atataaagtt tgcaagtcca
     5641 atgggcctct ctttccagtg atggccgact aggccatctt ttgatacata tgcagctaga
     5701 gtcaagagct ccggggtact ggttagttca taatgttgtt ccacctatag ggttgcagat
     5761 ccctttagct ccttgggtac tttctctagc tcctccattg ggggccctgt gatccatcca
     5821 atagctgact atgagcatcc acttctgtgt tttctaggcc ccggcacagt ctcacaagag
     5881 acagctatat cagggtcctt tcagcaaaat cttggtagtg tatgcaatgg tgtcagtgtt
     5941 tggaggctga ttatgggatg gctccctgga tatggcaatc tctagatggt ccatcctttt
     6001 gtctcagctc caaactttgt ctctgtaatg ttacaattct aaaatatgct attgttggtg
     6061 aatgaaagtg ctttgcttta aaatttagaa gtcagaataa agcaattata gagaaagcac
     6121 agttctagga tattgctgcc caggaacaag atgtacaact aagttactag acacatggga
     6181 atgggagatc aacctcatta gtcaccgtgg aactgcagat caaatggcag tcagtctcat
     6241 ttcacagata taatagtctc tagctaaaaa aaaaaaaaaa aggaatggat cctgaaaaga
     6301 tctatacact tgggatcttt cctggaaacc ttagtgctca actcctccac agttttgaaa
     6361 gccttgttac catccttagg gcaacccaga tgtgagctgc aggtctcctc ctgaacacca
     6421 gggggcgaaa ttggaccata taatcaacag aaaaaactcc tgagccatga ccaccccttg
     6481 tatattttct ttatttacat ttcaaatgtt ttcccctttc caggtctccc cttcagaacc
     6541 cccctatcct tacctccctc cccctacctc tatgaggatg ctcccacatc cacccattct
     6601 cattctcctg ccctggcatt cccctacact ggagtattga acagcctcag gcctgagggc
     6661 ctctcctccc actgatatcc aataaggcca tcttctgcca catgtttggc cagggccatg
     6721 tgtcactaat ggatacagaa aatgtggtac agccgggcgg tagtggcgca cacctttaat
     6781 cccagcactt gggaggcaga gacaggcgga attctgagtt cgaggccagc ctggtctaca
     6841 aagtgagttc caggacagcc tagactatac agagaaaccc tgtctcgaaa aacaaaaaag
     6901 gaagaaagaa agaaagaaag aaagaaagaa agaaagaaag aaagaaagaa agaaagaaag
     6961 aaagaaagaa agaaagaaaa tgtggtacat ttacacaaca gactactaat cagccattaa
     7021 aaacaatgac ttcatgaaat tcataggcaa atggatggaa cttgagaata tcatcctgag
     7081 tgaggtaact cagtcacaaa ggaacaaaca aacaagatat gtactcactg ataagtggat
     7141 attagtccaa aagttcagaa tacccaagat tcaattcaca gactatatga agcctaagaa
     7201 gaagaaagac caaagtgtgg atgcttcact gcttcttaga agggtgaaca aaatcttcac
     7261 aggaggaaat acggagacaa agtgtggagc aaagactgaa ggaaaggcca tccagagact
     7321 gccccacctg gggatccatt caacatatag ccaccaaacc tgtacacgat tgtggatgtc
     7381 aggaagtgct agctgatgga agccggatct gtctgtctcc taagaagctt taccagagcc
     7441 tgacaagtac agaggcaagg cgaaagctca cagtcaacca ttggactgag catggggtcc
     7501 cagatggagg agttggagaa gggactgaag gagccgaggg gggtttgcag cccagtggag
     7561 ggagcaacag tgccaagagg ccagaccccc acagagctcc cgggatctgg atgaccaacg
     7621 aaagaatacg caagaaccta tcttgcaacc tggattgcag tttccatccc agttgacaca
     7681 cctggagcca gctccctcac ctaaccatac tctgtcctcc tggctctttc ctgtatctct
     7741 gagaactcca ttctgcaagg acagccgatg tggtcccttc catcctccat catccatggg
     7801 agagatggga aatcccaggg cattcaccaa ggagaacaga gccatcctgg gacggttacg
     7861 gagggcacgg gcagacctga atcacatttg gccagcacag gaagctgggg aggtctccct
     7921 ggcaccctcc ataggaaggt ggagcacaca gtcctctttc caggacacac aggtcacctc
     7981 ctcctgcaca cccaggatat gaagcccctg agacaacttg tatcctagga tcagacacat
     8041 gactggtgtc atcagtgacg atggatcagg tcctacccag tcatcactca gctaggcctt
     8101 tccttaaccc tccagataac tctgccactt cctgcctgga gtaaacccca cctctgtgag
     8161 cattgagagc agggcacaga gggctcccat gggggtttgt gtcactctag gctacaggaa
     8221 atgctggaac tcctgctgca gttgacagcc ccaaggccag ggcacagggc actcctcagc
     8281 cttgctgctc ggagtatgtt ctagaacact gaactggaaa gaggaataga aggacgggag
     8341 gcccacactg acaggagttc agcattgtca gactcacagg ctccaccccc agcccacgtg
     8401 gatctgggag gtgccctccc ctgggaggag acaaagctcc tttaagaaaa gcagggcaga
     8461 tatcagggca gcctggctta gcagtagtgt tggagaagaa gctagcaggc aggcagcaga
     8521 gacatggagc tggcctcagc acatctccac aaagggcagg ttccctgggg aggactactg
     8581 ctcacaggta aggagatatt ccttcccagt agagagcagg ggagctcaga gactggctgg
     8641 gctcttctgg gagaggaaag gaacctgaga agggacatct ggcttctgct tgaagcttga
     8701 cggcaacagg aagctctcag tgagtgtgaa ttggctagtg gtgaggagta actcagttct
     8761 ttgctattgt taagtttatc gcactggcta ggatatcctg aagactgaag tccacaactc
     8821 tgtcagggtg actctccacg taaaaaccaa actggggcaa gtaaacagaa ttattgcata
     8881 ctacactgag aaatttgcaa atacactagg ctctgggaca ctgatttgca aacaggtata
     8941 tgagcatgtt ccaggcagca gggattaagc caaaacccac ctagccctgc acacaaagcc
     9001 ctagttctat gtgtaaaacg gcagacctgc gagggcatct aggtggggtc aggttggcaa
     9061 gcacttcaga aaaaatcaaa gcagagaaag gccagaaaat gaaggtgcag ggtctttaga
     9121 ggagggggtc agagaagata ggcctacact cagcaaagac tctgagtgtg agctggggtc
     9181 tgagggcagt gagaggtgag ctgtgtgaat gccggagaag agcgtttcct acagtggaaa
     9241 gatgaggaat ggaggtgatc tgctggccac cacccaatag gacacaggca cagcaaggct
     9301 gagaggtttt gcaaaggtcc taagattgat aggtctttct ctcttccctc ttagcctcac
     9361 ttttagcctc ctggagccct gccaccactg ctgaagtcac cattgaggct gtgccgcccc
     9421 aggttgctga agacaacaat gttcttctac ttgttcacaa tctgcccctg gcgcttggag
     9481 cctttgcctg gtacaaggga aacactacgg ctatagacaa agaaattgca cgatttgtac
     9541 caaatagtaa tatgaatttc acggggcaag catacagcgg cagagagata atatacagca
     9601 atggatccct gctcttccaa atgatcacca tgaaggatat gggagtctac acactagata
     9661 tgacagatga aaactatcgt cgtactcagg cgactgtgcg atttcatgta caccgtaagt
     9721 aattatctgt aacctcggcg tcatgggtag agttaatcct gcttcccaca cagcactgtc
     9781 aatccttgat gactcaacta tgtcccctac attgtggtgg gagcatttag ttcggggcac
     9841 acagagggga gaaatacagc aataaaccgc gatgcctcac ttggacccac tccttcagag
     9901 aagagattgt gaggtaactc agtgtgagga tgtcagactc tgcacagtaa cttaggctgc
     9961 ttatcctgag catggcctcg agaaagaccc ttcagggctc aagcagggcc tgcctgagga
    10021 aaccatgaga atctcacgga gaggtgagca ccaggaaact ttgctccaga cccagcctgg
    10081 actccaggaa ccctgggaag ttgttagcct gggttgagat ttgccctctt gttagagctg
    10141 acagggacaa cggttgattg taggctggta ggctgttgac agtcagccta gactagagcc
    10201 atgtctgagg taggtcacct gggtatctcc ttctgagacc cagtgtgctc tgcgtacatg
    10261 gagaggtatg aaagacaact agccaactgt tgtgatgttc ctgtgagact tcccaggaaa
    10321 atcgagagcc ctaaaatgat aggaggaaac cgtgtacaaa tggcagctcc ttgctctcca
    10381 gggtgtcaag cccagggatt atttcctgca gcaaatagta acagactctg gtctatggaa
    10441 ggtcagatca ttgctccttt taaggttcat ttgcaggcat ggcaatgatc atttaccata
    10501 tgtcctatta tggggaaact gaggcagtag atacagtcac tgagtcaggg tcacataggc
    10561 cctgggtggc atgagtttat ctgtccagag ccacatctcc agcctcccca ccctggagat
    10621 cttattaggc acttgttatt cttttagata atttcttgca ctcaggcttc tcttttgggg
    10681 gatctcttct tactcatgtc agtcaacacc aaacacccga caaaattaag gcgcataacg
    10741 ataccacgat atcaacaagt ttgtacaaaa aagcaggctg gcgccggaac cgaagttcct
    10801 attccgaagt tcctattctc tagaaagtat aggaacttcg aaccctttcc cacaccaccc
    10861 tccacacttg ccccaaacac tgccaactat gtaggaggaa ggggttggga ctaacagaag
    10921 aacccgttgt ggggaagctg ttgggagggt cactttatgt tcttgcccaa ggtcagttgg
    10981 gtggcctgct tctgatgagg tggtcccaag gtctggggta gaaggtgaga gggacaggcc
    11041 accaaggtca gccccccccc cctatcccat aggagccagg tccctctcct ggacaggaag
    11101 actgaagggg agatgccaga gactcagtga agcctggggt accctattgg agtccttcaa
    11161 ggaaacaaac ttggcctcac caggcctcag ccttggctcc tcctgggaac tctactgccc
    11221 ttgggatccc cttgtagttg tgggttacat aggaaggggg acgggattcc ccttgactgg
    11281 ctagcctact cttttcttca gtcttctcca tctcctctca cctgtctctc gaccctttcc
    11341 ctaggataga cttggaaaaa gataagggga gaaaacaaat gcaaacgagg ccagaaagat
    11401 tttggctggg cattccttcc gctagctttt attgggatcc cctagtttgt gataggcctt
    11461 ttagctacat ctgccaatcc atctcatttt cacacacaca cacaccactt tccttctggt
    11521 cagtgggcac atgtccagcc tcaagtttat atcaccaccc ccaatgccca acacttgtat
    11581 ggccttgggc gggtcatccc cccccccacc cccagtatct gcaacctcaa gctagcttgg
    11641 gtgcgttggt tgtggataag tagctagact ccagcaacca gtaacctctg ccctttctcc
    11701 tccatgacaa ccaggctccc aggtcccgaa aaccaaagaa gaagaacgca gatcgcagat
    11761 cgcagatctc tagagtcacc gagttgcttt accggatgaa gagggccgaa acatactgtc
    11821 caaggccctt gctggcaatc cacccaactg aagccagaca caaacagaaa attgtggcac
    11881 cggtgaaaca gactttgaat tttgaccttc tcaagttggc gggagacgtc gagtccaacc
    11941 ccggaccggt gcatgtggac cccaagaaaa agcggaaggt ggaccctaaa aagaaacgca
    12001 aagtggatcc taagaaaaaa agaaaggtgc acgtgtccat ggtgtccaag ggcgaggaac
    12061 tgttcaccgg cgtggtgccc atcctggtgg aactggatgg cgacgtgaac ggccacaagt
    12121 tcagcgtgtc cggcgagggc gaaggcgacg ccacatacgg aaagctgacc ctgaagttca
    12181 tctgcaccac cggcaagctg cccgtgcctt ggcctaccct cgtgaccaca ctgacctacg
    12241 gcgtgcagtg cttcagcaga taccccgacc atatgaagca gcacgacttc ttcaagagcg
    12301 ccatgcccga gggctacgtg caggaaagaa ccatcttctt taaggacgac ggcaactaca
    12361 agaccagggc cgaagtgaag ttcgagggcg acaccctcgt gaacagaatc gagctgaagg
    12421 gcatcgactt caaagaggac ggcaacatcc tgggccacaa gctggagtac aactacaaca
    12481 gccacaacgt gtacatcatg gccgacaagc agaaaaacgg catcaaagtg aacttcaaga
    12541 tccggcacaa catcgaggac ggctccgtgc agctggccga ccactaccag cagaacaccc
    12601 ctatcggcga cggccctgtg ctgctgcctg acaaccacta cctgagcacc cagagcgccc
    12661 tgagcaagga ccccaacgag aagagggacc acatggtgct gctggaattc gtgaccgccg
    12721 ctggcatcac cctgggcatg gacgagctgt acaagcctgc aggcgagggc agaggaagtc
    12781 ttctaacatg cggtgacgtg gaggagaatc ccgggccgtc caatttactg accgtacacc
    12841 aaaatttgcc tgcattaccg gtcgatgcaa cgagtgatga ggttcgcaag aacctgatgg
    12901 acatgttcag ggatcgccag gcgttttctg agcatacctg gaaaatgctt ctgtccgttt
    12961 gccggtcgtg ggcggcatgg tgcaagttga ataaccggaa atggtttccc gcagaacctg
    13021 aagatgttcg cgattatctt ctatatcttc aggcgcgcgg tctggcagta aaaactatcc
    13081 agcaacattt gggccagcta aacatgcttc atcgtcggtc tgggctgcca cgaccaagtg
    13141 acagcaatgc tgtttcactg gttatgcggc ggatccgaaa agaaaacgtt gatgccggtg
    13201 aacgtgcaaa acaggctcta gcgttcgaac gcactgattt cgaccaggtt cgttcactca
    13261 tggaaaatag cgaccgctgc caggatataa ggaatctggc atttctgggg attgcttata
    13321 acaccctgtt acgtatagcc gaaattgcca ggatcagggt taaagatatc tcacgtactg
    13381 acggtgggag aatgttaatc catattggca gaacgaaaac gctggttagc accgcaggtg
    13441 tagagaaggc acttagcctg ggggtaacta aactggtcga gcgatggatt tccgtctctg
    13501 gtgtagctga tgatccgaat aactacctgt tttgccgggt cagaaaaaat ggtgttgccg
    13561 cgccatctgc caccagccag ctatcaactc gcgccctgga agggattttt gaagcaactc
    13621 atcgattgat ttacggcgct aaggatgact ctggtcagag atacctggcc tggtctggac
    13681 acagtgcccg tgtcggagcc gcgcgagata tggcccgcgc tggagtttca ataccggaga
    13741 tcatgcaagc tggtggctgg accaatgtaa atattgtcat gaactatatc cgtaacctgg
    13801 atagtgaaac aggggcaatg gtgcgcctgc tggaagatgg cgatctcgag ccatctgctg
    13861 gagacatgag agctgccaac ctttggccaa gcccgctcat gatcaaacgc tctaagaaga
    13921 acagcctggc cttgtccctg acggccgacc agatggtcag tgccttgttg gatgctgagc
    13981 cccccatact ctattccgag tatgatccta ccagaccctt cagtgaagct tcgatgatgg
    14041 gcttactgac caacctggca gacagggagc tggttcacat gatcaactgg gcgaagaggg
    14101 tgccaggctt tgtggatttg accctccatg atcaggtcca ccttctagaa tgtgcctggc
    14161 tagagatcct gatgattggt ctcgtctggc gctccatgga gcacccagtg aagctactgt
    14221 ttgctcctaa cttgctcttg gacaggaacc agggaaaatg tgtagagggc atggtggaga
    14281 tcttcgacat gctgctggct acatcatctc ggttccgcat gatgaatctg cagggagagg
    14341 agtttgtgtg cctcaaatct attattttgc ttaattctgg agtgtacaca tttctgtcca
    14401 gcaccctgaa gtctctggaa gagaaggacc atatccaccg agtcctggac aagatcacag
    14461 acactttgat ccacctgatg gccaaggcag gcctgaccct gcagcagcag caccagcggc
    14521 tggcccagct cctcctcatc ctctcccaca tcaggcacat gagtaacaaa ggcatggagc
    14581 atctgtacag catgaagtgc aagaacgtgg tgcccctcta tgacctgctg ctggaggcgg
    14641 cggacgccca ccgcctacat gcgcccacta gccgtggagg ggcatccgtg gaggagacgg
    14701 accaaagcca cttggccact gcgggctcta cttcatcgca ttccttgcaa aagtattaca
    14761 tcacggggga ggcagagggt ttccctgcca cagcttgatg aagatctgag ctcttaatta
    14821 atgatcataa tcagccatat cacatctgta gaggttttac ttgctttaaa aaacctccca
    14881 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
    14941 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
    15001 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
    15061 atccgtttaa actaacttta aataattggc attatttaaa gttacatatg gcggccgccc
    15121 taaggctcga cctcgaaatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga
    15181 gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc
    15241 acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg
    15301 ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc
    15361 aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct
    15421 gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct
    15481 ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg
    15541 ggcggggcgg gcgcccgaag gtcttccgga ggcccggcat tctgcacgct tcaaaagcgc
    15601 acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg catccatcta
    15661 gatctcgagc agctgaagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc
    15721 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
    15781 acgcgccaca ccgtcgatcc agatcgccac atcgagcggg tcaccgagct gcaagaactc
    15841 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
    15901 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
    15961 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
    16021 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
    16081 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
    16141 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
    16201 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
    16261 atgacccgca agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg
    16321 agcgcacgac cccatgcatc gatgatatca gatccccggg atgcagaaat tgatgatcta
    16381 ttaaacaata aagatgtcca ctaaaatgga agtttttcct gtcatacttt gttaagaagg
    16441 gtgagaacag agtacctaca ttttgaatgg aaggattgga gctacggggg tgggggtggg
    16501 gtgggattag ataaatgcnt gctctttact gaaggctctt tactattgct ttatgataat
    16561 gtttcatagt tggatatcat aatttaaaca agcaaaacca aattaagggc cagctcattc
    16621 ctcccactca tgatctatag atctatagat ctctcgtggg atcattgttt ttctcttgat
    16681 tcccactttg tggttctaag tactgtggtt tccaaatgtg tcagtttcat agcctgaaga
    16741 acgagatcag cagcctctgt tccacataca cttcattctc agtattgttt tgccaagttc
    16801 taattccatc agaagctggt cgagtaactt taaataattg gcattattta aagttaggcg
    16861 cgccataact tcgtatagca tacattatac gaagttatgt cgagatattt aaatattaga
    16921 cccagctttc ttgtacaaag tggttgatat ctctatagtc gcagtaggcg gcagctgtcc
    16981 tggaaaatgg agggagtcga cagattgtgt ctaaaggcag acaaccagtc agtgagtacc
    17041 aggacataag aggaggtctc tgattctgag acttggatct gtgcatcaac aaactgcagc
    17101 tcagttactc aggagagagc cttgcttctc ccatacacag tgcaggagac cccatcctct
    17161 ctgagcactc agatactcca tctgcctata atttgcctcc tgcctggttc tctaaggatc
    17221 tgggttggca gggaggtaag agaccagctt ggattgaaag aggccattgt aaagatcaga
    17281 ggtaccacac agggcaatct tctctctatt atctgcacag ccatattatt aaagcccaac
    17341 atcacaagca acaactccaa tcccgtggag ggtgacgact ccgtatcatt aacctgtgac
    17401 tcttacactg accctgataa tataaactac ctgtggagca gaaatggtga aagcctttca
    17461 gaaggtgaca ggctgaagct gtctgagggc aacaggactc tcactttact caatgtcacg
    17521 aggaatgaca caggacccta tgtgtgtgaa acccggaatc cagtgagtgt caaccgaagt
    17581 gacccattca gcctgaacat tatctgtgag taacttcttc cccatccttg ctacagctgc
    17641 ctaaatacag agagctagag ccgggacaca cattcgcttc tgggttcatg acagacccac
    17701 cacatatata tatctaggtg ggccatgata tgctggccta gcccaagcac atgaagccag
    17761 gcgcagtcct gagctagaat gaactggagg gattactcct atctagagag gatcagggtg
    17821 atggagacac aggggacatc tcagactcag gctcagcgaa cacttgagtg tccagtgggg
    17881 ctgttctctg tgtgttagtg atggaccagg acgcatgcac actacatgag tacccttcta
    17941 ttaaccttca cccaggctct ggctgggacc tgcttaactg aagttatagc acagatcctg
    18001 tagccttcta gagaccagga tttccccttc cctctgctga cgtcacattt ggctttattc
    18061 tgtttgctcc agatggtccg gacaccccga ttatatcccc ctcagatatt tatttgcatc
    18121 cagggtcaaa cctcaacctc tcctgccatg cagcctctaa cccacctgca cagtactttt
    18181 ggcttatcaa tgagaagccc catgcatcct cccaagagct ctttatcccc aacatcacta
    18241 ctaataatag cggaacctat acctgcttcg tcaataactc tgtcactggc ctcagtagga
    18301 ccacagtcaa gaacattaca gtccttggta agtggatctc tgaagtatca gcaccagggt
    18361 tggagtggag tcttttggtg ttctgggaag gtgcaaacca gcatcatttc cagcctgttt
    18421 ctatgggaac aggcaaaatg caatcctccc cagagctgct tcctccatcc ctgtatctaa
    18481 cccctttctg tttctcctgg ctgatctggc attgaacttg gaatggagga ggaggttctc
    18541 ccagccttga aaagcctcag gtggtgtaag ggacttccca agggagggag atttccttct
    18601 gtcactaacc catgccttct gccatttcca tcttcctttg gtgatctcca tgactgagaa
    18661 ggaacactca gggctttgaa ctatgcgcac attcattttc tccaagtgag aggagaaaaa
    18721 tcccctcaga tggggaccag cagctctgag ctctgctccc agatctgttc ctgtctccct
    18781 gagtgatggg atgggaagac tgggttacag gagataactg ggagccctgt gctgagatgg
    18841 ggtgaaccac gttgcaggga atttgatgat gctttttcac ttgctcttct caaggtttaa
    18901 aaataaacat aggctgagtg tggtggtgca cacctctaac cccagcactg aggagggagg
    18961 ggcaggggga gatttatgag attgaggcca gcctggtaca catagtgagt tccaggccag
    19021 cctcagctgc atagtgagat cctgcctcaa aaatcataaa taaataaata aataaataaa
    19081 taaatagtga aagaaaaggc cactggtggt cagcaaagag tacatgggga tttgcttgtt
    19141 cccaaagact ggaaaagtga aaaagtgaac tttggggcag ttggctctga actgtgtcct
    19201 ggctctaaag gtccagctgt gtgagcccca gacaccagca ctgggtgatc cctgagaaca
    19261 gtgaagggca ctggcttgca gaacagggac cttcctgttg agtttctgtg tagaggaaag
    19321 gacaaggaaa gcacctggtg actctgtggt atgctccatg tgtggtggct cacacctcta
    19381 atcccagcac tcagagggct gaagcagatg ggtctctgag tttaaggcta gcctggtcta
    19441 cagaccacgt tctaggctat ctaggactgt acagtagtga gaccttgtct tttagagaaa
    19501 tctgtagtga gcaccagagt ggcaagctgt tgataacact gtgaccattc acttcctcac
    19561 tgtggtattt agtccctatc catggggcaa ggaaataaca tttttcttct catagaaatt
    19621 ctttagtttg ggatttctcc cagtcctgtg gtctaggctt tgacatcttt aagattagct
    19681 gggccaactt aaccgcatga caaattaatt acagaataga gagctatttt gtgacagagg
    19741 tacctgatac cgcactcact gaaagttagc acaaaaaaaa atcattggga aaaacattgc
    19801 tccctggtga ggtttctgga tttatggaat ggagaggctc ctggtgagga atctatacaa
    19861 atgccctgag acaagggtca ctctgagtga tctgggtagg aggagtttac atctctctcc
    19921 ttacagatct taccttgaac tctaacatgt gaccctacag aggaccgttt cattctgtgg
    19981 gccctgtgag ttggttgaga tctctaagga tgaaggactc tcagctggtc ccctcctctg
    20041 tttgtccaca gagccagtga ctcagccctt cctccaagtc accaacacca cagtcaaaga
    20101 actagactct gtgaccctga cctgcttgtc gaatgacatt ggagccaaca tccagtggct
    20161 cttcaatagc cagagtcttc agctcacaga gagaatgaca ctctcccaga acaacagcat
    20221 cctcagaata gaccctatta agagggaaga tgccggcgag tatcagtgtg aaatctcgaa
    20281 tccagtcagc gtcaggagga gcaactcaat caagctggac ataatatgtg agtgaccttg
    20341 ccacccttct tattacagag tgttgacggt gggtgcaatt tatcaagggt gtgaaaaatg
    20401 ggtaaaagtt tcaaggagaa aaatacatct caagatacaa atacagccac catggcgatg
    20461 gatgcgaatc aattcacagt aaaacacata ataagctaag catggcacct ttaatcccag
    20521 cacaccatag ggatctctga gttcccaacc agccttgtct acatagcaag ttccaggtta
    20581 ccctgcaaat acagactaca agtctttgag atggcgcaac gcaattaatg ataacttcgt
    20641 atagcataca ttatacgaag ttatggtctg agctcgccat cagttcattg tttttccact
    20701 agtgttggag tttcttgcac attctgaata ttaacctctt gtcagatgtt ttacttacaa
    20761 atatattctg ctattctaaa agcggacttt tttgtcactt tcctggtaaa agcctttgtt
    20821 tattattagt aatggttttt aaattggcac aaaaattgaa tacatttaag tgaaattata
    20881 tctagataca ggaatgatta aaacaagttg attgacacag ttgttacttc ctttatactt
    20941 tttatgctaa gacatttaaa atttattgtt catttctttg caatacatta tggctgacta
    21001 taattcccct gatgtgtcag acctgtcaaa acctaacctt ctggtctgtc ttaaaccttg
    21061 gaccctgacc agtgactccc catcgctcct gctccacgaa gtctctggtc agcttcattc
    21121 cactctctcc ttccacattg ttgagatcat ggaagagact gtgtggagtt tcccacgcaa
    21181 ggcctgtctc acccagcatc ctgtcctcta ggctctttca cagaatcaca tgacagagct
    21241 gcctcctttt aaaggtccag tggcactcct atgtgaagat acattcacat tttcttactt
    21301 attcatccac tgatagacat ttactgtcca tgccttgatt attgtgaaaa gtgccacaaa
    21361 gacctgctgg gctgtatgac tgataagatc ctctgatgca caaacatttt caattttgct
    21421 aaagtccact ttatctattt ttatcatggc cctatgcatc tggcaccatg acagagaaac
    21481 tgagccaaat tcaacctcaa gaagcttcca tcctgttttc taaaagtttt atggttggtg
    21541 ttcggctctg atattagggt ctttgataca cttagaattg atttttatat gatgtaaggt
    21601 gatggcttag aaacaatcac tcttttccct ggatatccca tttccccaat cccttgttta
    21661 aaagataatc cttctatcat tgagcaatct tagcctgatg aaaactcttc tggtcccagg
    21721 tgcagttgga cagcaagaat gctttccaaa ttctgtaact ggagaggaca cctctgcttt
    21781 acaccaacta actctttcaa aagagccagg agagatgaat gttcataaca ccaaaaaatg
    21841 acaaatgaag tgagagatgt gccatcctct ctgatctgac agcacattat agtatacatg
    21901 caaaacatat acagtggccc ataaacatgc acatttatca tgttcaacta atcttgaatt
    21961 gaatcattgg ccagtgtaca tgagcagttc tgagctgtct attctatttc actactctat
    22021 atgtctgctt ttctgtcaga cagttcggtt attgtggatt tgtactaaaa ttattgttgt
    22081 agaaaatttt aatcacaatc tggagtttct actccacctt tgatcattca gttctcagaa
    22141 aaaagacaca caacttttat tatttacact aagccttaat ctgtactaga actggacaga
    22201 tatctaccct ctatgctatt aaaatctatt tcctgtcaat aaccccaaga tattacttaa
    22261 tacgtttcat ctgggctgat cttaactcca attggccagg cctcagggcc atgttttctt
    22321 gactcctaac ccatggcgac atttttttcc cttaccatca tcctccttcc tcctccccct
    22381 cattttccta ctccttccct ccctccctcc atccccaccc caccccaccc cccgttttcc
    22441 ctggatccct tagcaggaac cctaaaaccc acctatgtct cttctgccca gctattggct
    22501 gttggcatct ttatgtacca gtgagaaata actgtctggt ctctggggca accatgtctt
    22561 gggggcccac acttagcatt tcaatacaca gcaaattggc tacagccaat tactggaggg
    22621 attagagggt ggactagaag tacctggtta gagaaggagg gggagatgga caatgagcca
    22681 tcccacggcc aggagaaaca gctagtatct agggttcacc actgtggagg tagacaggcc
    22741 agcagcagaa tagattatgg gtaatcctat agtaattgcc aaagccaaat aaaataacca
    22801 tagcctgagt cttgtttata tgtaagctag tcggggatag gcttaaattg attgtactat
    22861 aattatgaaa tcatgaagga tgtccatgca attttcttcc tccttttcag gatactttta
    22921 aggttaggtg tgatggcact tccctctaat ccctgatctc aggaggcaga gatagctgga
    22981 tctctgagac attgaggcca gcctagtcta tatagtaagc tccaggacag ctagaataga
    23041 catttctatt tctgtataaa atatcactgg ggttttaagc caatgtgcat aggactgaca
    23101 gaacaccata gtaatatcat catctgtctt aaaaagtctg tcagacagtc tggctcatga
    23161 ctgtgatacc ggccactcga gaagctgagg cagaagtacc aaggaagagt gcctaacata
    23221 actagttcaa ggccaccctg agaaatttcg tggcacgctg tttcaaaaca gacgttaaaa
    23281 ggcagagctc aggatatagc tcagtggtag agcatttttt actatgctca a
//