LOCUS       91578#pL1L2_GT1_LF2A_nEGFPO_T2A_CreERT_puro#L3L4_pD223_DTA_T_spec        21909 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT1_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 91578
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000027012
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            8691..8945
                     /db_xref="ENSEMBL:ENSMUSE00000305800"
                     /rank="2"
                     /note="ENSMUSE00000305800"
     misc_feature    4275..9789
                     /note="5 arm"
     misc_feature    9817..9841
                     /note="B1 Gateway"
     misc_feature    9854..9901
                     /note="Frt"
     misc_feature    9902..10401
                     /note="mouse En2 intron"
     primer          complement(9970..9991)
                     /note="LRPCR LAR3_EN2"
     primer          complement(9866..9889)
                     /note="FRT3"
     primer          14067..14085
                     /note="seq primer NF"
     misc_feature    14127..14134
                     /note="RE sites"
     misc_feature    15927..15960
                     /note="loxP"
     misc_feature    complement(14135..14166)
                     /note="Rox"
     misc_feature    complement(15887..15918)
                     /note="Rox"
     misc_feature    14167..14188
                     /note="polylinker"
     CDS             14747..15343
                     /note="puromycin N-acetyl transferase"
     misc_feature    14862..14867
                     /note="Mutated RsrII site"
     intron          10402..10776
                     /note="mouse En2 intron"
     misc_feature    10774..10776
                     /note="Splice Acceptor"
     exon            10777..10830
                     /note="mouse En2 exon"
     primer          10677..10698
                     /note="seq primer FCHK2"
     misc_feature    10831..10953
                     /note="1Dleader"
     misc_feature    10954..11005
                     /note="F2A"
     misc_feature    11101..11817
                     /note="EGFP"
     misc_feature    11011..11100
                     /note="3X NLS"
     misc_feature    13883..14121
                     /note="SV40 polyadenylation site"
     misc_feature    12916..13860
                     /note="ERT2"
     promoter        14199..14710
                     /note="PGK promoter"
     misc_feature    15454..15886
                     /note="PGK pA"
     primer          13941..13964
                     /note="LRPCR RAF5"
     misc_feature    11827..11880
                     /note="T2A"
     misc_feature    11877..11877
                     /note="A to G to kill RsRII site"
     misc_feature    11881..12906
                     /note="Cre"
     misc_feature    10792..10831
                     /note="FCHK lf2a_lacz_gt1"
     misc_feature    10792..10807
                     /note="FCHK QC prefix region"
     misc_feature    10808..10825
                     /note="FCHK QC critical region"
     misc_feature    10826..10831
                     /note="FCHK QC suffix region"
     misc_feature    15983..16003
                     /note="B2 Gateway"
     misc_feature    9790..16033
                     /note="Synthetic Cassette"
     exon            16412..16509
                     /db_xref="ENSEMBL:ENSMUSE00001312458"
                     /type="targeted"
                     /rank="3"
                     /note="target exon 1 ENSMUSE00001312458"
     exon            17217..17390
                     /db_xref="ENSEMBL:ENSMUSE00001263181"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 2 ENSMUSE00001263181"
     misc_feature    16034..17851
                     /note="Critical Region"
     misc_feature    17875..17908
                     /note="Downstream LoxP"
     misc_feature    17852..17931
                     /note="synthetic loxP region"
     exon            18322..18556
                     /db_xref="ENSEMBL:ENSMUSE00001292356"
                     /rank="5"
                     /note="ENSMUSE00001292356"
     exon            20774..20917
                     /db_xref="ENSEMBL:ENSMUSE00001281655"
                     /rank="6"
                     /note="ENSMUSE00001281655"
     misc_feature    17932..21909
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggatatttc gatattttta tatctgctga tgcctgtttg gcgactgatt
     4321 gtatggtaaa ttttggagaa ggtaccatga ggtcctgagt agaaggtata tccttttgtt
     4381 ttaggataaa atgttgtgta gatacctgtt caattcattt gtttcataac ttctgaagga
     4441 agttttcttc tgtaattttg ttgaagatat ttactgtccc tttaagttga aaatcttcct
     4501 tcttgtctat gcctattatc cttatgcttg gtcttttcgt tgtgtcttgg gttttctaga
     4561 tgtttggggt taggatcttt ttgcatttag cattttcggt gattgtagtg tcaatgcttt
     4621 ttatggtatc ttctgtacct gaaattcact cttctatctc ttgtattctg ttggttatgc
     4681 ttgcatcaat gtctcctgac ttctttccta ggttttcgag agtggtctcc ctttgtgatt
     4741 tctttattgt tttacttcca tttttagatc ctggatggtt ttgttaaatt ctttcacctg
     4801 attggatgtg ttctcctgta tttctttaag ggagttattt atttccttct tatagtcatc
     4861 catcgccatc atgagaagtg actttagatc catatcttgc ttttctggtg tgatggtata
     4921 tccaggaatt gctatggtgg gagagtttgg ttctgatgat acaaagtaac cttggtttct
     4981 gttgcttctg ttctgatgct tgcctcctac catctgatta tctcaaatgc ttgctgccct
     5041 caatatatct gattggagcc tgtccttcct ataatccagg ttgattccaa acacctcaga
     5101 gtccagcttt ctctgggatc ctgtgattct gggatcctgt gaacctgaga ttctgggtgt
     5161 gtcagagttc ttagcagtca agcttcctct gagaccctga gttcctggtg tgaccaagct
     5221 cctgggatcc tggaatccta agacccttgt catattacag tgcctggaag tgttaactcc
     5281 tctgggtaca atggggttgt ctgctgtgtt caaaacctag gtagaccagc accaactaaa
     5341 aggaactcta tacgctggtc agtcaggggg cacctgtgcc cttgcttctg ctgtcacatg
     5401 cccctcgtga ttgttttggg accaatgttg agttccactc accagtgatc ctaggatcct
     5461 ggaagttcta ggtctcctgc agcatggaga gttctctggg gactgtggga ccatctgctg
     5521 actttgcacc caaggtggtc tggagctggc gctgactaga gggaacccaa acagcttctt
     5581 taatgggttt cctgtgtccc tgttcctgct tgcacaggcc cctcgctatt ttttttggaa
     5641 cagatgttgc atttcactca ccaatgatcc taagaacctg ggtgtggaga gtcctctggt
     5701 aactgtgggc ctgtccacag agtttgcaca caaggcggtc taggaccggt gcagactgga
     5761 gtgaacctct ctatctcttt ctaaaccaaa aaaaaaaaaa accaaaaaaa aaaaaaaaaa
     5821 aaaacctccc cccaaaatgt caagcgaaat aaatctaact agaaaataaa aaactagaaa
     5881 gaagcgcaaa aaaacatatg cacacaagta aatctaatat aaaaataaaa ttaaaagcta
     5941 taatataatc aaaatataag taatattaaa aaaagcaata tggaacaaaa tgtcaaaata
     6001 tatacacttg aatttatttt gtattggaca ctgacagctg ggcatgtgat gaaccttaag
     6061 tgtggtttgt atatccaatg agagtccatt ggagaaagct attttttgtg tggggggtta
     6121 gttttattaa ttttatttat atatcataga atacacgtat acatacacaa actcataaga
     6181 aagatgcaag ctttgtaaaa atgaattgaa agggccttct tccctttttt aagatatttt
     6241 tattagatat tttcttcatt tacatttcaa atgctatccc taaagtcccc tatactctcc
     6301 ccccaccttg ttcccctacc cactcactcc cacttcttgg ccctggtgtt ccactctact
     6361 ggggtatata aagtttgcta gaccaagggg cctctcttcc cactagggag aaagctaatt
     6421 tttaagtcat ataatctact agtagaataa gtagttcgtt atttattatt attattatta
     6481 tgtggtaaga atgttgaaac aggttctccc tatatatcca agtctgcctc atatctgtag
     6541 ccatccttct gtctcagttt tccaagtttg agaattctga tgcgaaaatc attacaccta
     6601 ttagtttcta tagcttttgt aaaatgcttg aagttcctgt ttataagacc caatctgttt
     6661 ctctagaatc agctgcttcc atttttttct gatttctaga attctcaata ttttatggaa
     6721 caaaagtata gagggtaact ttttttctgg tcatagtaaa atttaggtta tttgtgacca
     6781 tcgaatatga tgttaaacat ggcgaattac acgccacaga catatatgta ttttttttat
     6841 aattttaatc tctttagtcc ctactattga tcacccattc agcactgttc ttcttctttt
     6901 gaatataaaa attactataa cagagaacca aagctcaggc caaatagatg cttcaacact
     6961 aacctgagtt ttatttacat tatcaaagtg tgagatgaca tttatagact atcatccaag
     7021 accaacatct cttcatctct tctatataat agagacactt taaacacagt ttttcctctt
     7081 ttctatgtcc tgaaacatat tactagtatc tttccaagta tctctttacc aaaagacatg
     7141 gaacaccata tgctgttata aaagagtgtc tctaacatgt gagctaacat gttctgttta
     7201 atttatagta tttttatgat ttgatcaatt aatagctaag ttctctgtgt atagggtgtc
     7261 ttactaatat tcttttcaca aagatttttt tgtgtgacat ttttggaaaa acattcatct
     7321 aaattaattg gtgcaatggc aaatcagcaa atttttattg ttatagagcc tatgaaatgg
     7381 ttgagtatgt attatctttt tttaaaatat tttttattag gtattttcct tatttacatt
     7441 tccaatgcta tcccaaaagt cccactcccc tagagtatgt attatcttaa tttatatact
     7501 ttatagtaac ttgttaatga actaatatta tatttatttg tgaagatcat aaaataagta
     7561 atattattac tgtacaggct tactgtgaag gtccggtata tcaatatgaa caaaaatctc
     7621 aacctacaca ttttacctca cagtggtttt gttgtctttt attgtttatt ttttgggggg
     7681 tgggtgggta cttccacgtt tagtcataag tgtagagtag tgttatacct attgagttaa
     7741 ctgatattag gtactttttt atgtattata attcctatgt gtctttttgc agaataaatg
     7801 cccagaaaat gctgaaaaga aaatacaaaa caataaagaa aataagcatt gtgatctggg
     7861 ccaaagccct tgtttactat gtagttaaat attcttttac tatgccattt tcatgtgaaa
     7921 gttattttga cattttcact aagtaattaa tatggcaatc ttaagtgaga attacttaaa
     7981 tattctgtct ttatatatac ttcatgctca aatcttattt taaaaattaa aattaaaaat
     8041 ggtactttga tatctctgtt ttactaccct ataaaaatgt atccatgcta aggagtaata
     8101 tataactctt aattttgcta taccagaata ttaatattta taatatattg atttctaatt
     8161 tctgtttatg aacaaaatga aataattaga aattttgatg catttgttta taattaatgt
     8221 ttaaaaataa atcaattaat gtattaagca caaacagctg agtacaatat aagtttaata
     8281 taattagaat aaaaagcata ctgctttaac tgaactaaat acaaaggagt gactctacag
     8341 actagtgaga acaatttcct gtcttgacaa gagctttgcc ttcccccaaa aacacacatt
     8401 ctcttcagaa ttgaccaaga cattggtaaa aatcaaacca atgaatgacc aatgctatac
     8461 aataaagaga agcagctact gaaaagcctg catagtgatg ccctaataag catagactgc
     8521 tgcaaacttg caaggttaac ccatttctcc aggtacaatg aaataggcat gtagcttcct
     8581 ggtaaatggg aaaagagagc ttgtttttta gaaaagttgt tagctcacaa agactaagtt
     8641 gattccaagc tctacctgtt gtagttgaac aagtcttttg tctctgacag aaaacaaaca
     8701 ccctgacaaa ccacttaagg tgttggaaca gctgggcaaa gaagtcctta cggagtacct
     8761 agaaaaatta gtacaaagca atgtactgaa attaaaggag gaagataaac aaaaatttaa
     8821 caatgctgaa cgcagtgaca agcgttgggt ttttgtagat gccatgaaaa agaaacacag
     8881 caaagtaggt gaaatgcttc tccagacatt cttcagtgtg gacccaggca gccaccatgg
     8941 tgaaggtaag agcagaatgt actgtgaaca acagctcagg gtgctcatga tctgttcgat
     9001 tttcaggccc aacacttgct ttggtctgct aatctttgtg tcactatcac ttttctccaa
     9061 aatagataat actttagaaa gtgtgttgac tttcataggt aggtctcaga atccaacgtg
     9121 gtaaaaaagc taagtccttt gttcaagcta ttttgagaaa tagctagtca tctgacaatt
     9181 ataaaatgga acactaatcc taaagtttat aaattttgtg gctctcatca gttttagctt
     9241 atggaaaatg ggtgccttct aatactgtct ctcatgtttt tccaaagtta gaaacatagt
     9301 caagccatga acacaacttc ccaaatgctg gctctcatcc aaggtaattt tctggactac
     9361 atactcactg aacactgcct ccatagttaa tatttctgag cacatgttcc atttttttaa
     9421 tgaaatgaac acctgtaaaa ctaaacagtc ttcttccatt aagccaatta gatgcattca
     9481 cacaaactga aagaatctca agcataaaac taaactaaaa aaatattata tccaataagg
     9541 atcagaattg gaggaaggtg ttcaaaatac ttttagtaat ttcaggaatt ccttacatgt
     9601 tatatcaatt aatagggttt cttatcagtt tatacgaatc aaaaattaaa aatttatcta
     9661 cattcctatg ttttcaaatt tcttcccctc caagactgta gctaggagcc acttgagcat
     9721 agtaacgcat gccaaaatgg caaaaatctg tgatgcattg cactgaagag taagagtatt
     9781 actaaaatta aggcgcataa cgataccacg atatcaacaa gtttgtacaa aaaagcaggc
     9841 tggcgccgga accgaagttc ctattccgaa gttcctattc tctagaaagt ataggaactt
     9901 cgaacccttt cccacaccac cctccacact tgccccaaac actgccaact atgtaggagg
     9961 aaggggttgg gactaacaga agaacccgtt gtggggaagc tgttgggagg gtcactttat
    10021 gttcttgccc aaggtcagtt gggtggcctg cttctgatga ggtggtccca aggtctgggg
    10081 tagaaggtga gagggacagg ccaccaaggt cagccccccc cccctatccc ataggagcca
    10141 ggtccctctc ctggacagga agactgaagg ggagatgcca gagactcagt gaagcctggg
    10201 gtaccctatt ggagtccttc aaggaaacaa acttggcctc accaggcctc agccttggct
    10261 cctcctggga actctactgc ccttgggatc cccttgtagt tgtgggttac ataggaaggg
    10321 ggacgggatt ccccttgact ggctagccta ctcttttctt cagtcttctc catctcctct
    10381 cacctgtctc tcgacccttt ccctaggata gacttggaaa aagataaggg gagaaaacaa
    10441 atgcaaacga ggccagaaag attttggctg ggcattcctt ccgctagctt ttattgggat
    10501 cccctagttt gtgataggcc ttttagctac atctgccaat ccatctcatt ttcacacaca
    10561 cacacaccac tttccttctg gtcagtgggc acatgtccag cctcaagttt atatcaccac
    10621 ccccaatgcc caacacttgt atggccttgg gcgggtcatc ccccccccca cccccagtat
    10681 ctgcaacctc aagctagctt gggtgcgttg gttgtggata agtagctaga ctccagcaac
    10741 cagtaacctc tgccctttct cctccatgac aaccaggctc ccaggtcccg aaaaccaaag
    10801 aagaagaacg cagatcgcag atcgcagatc tctagagtca ccgagttgct ttaccggatg
    10861 aagagggccg aaacatactg tccaaggccc ttgctggcaa tccacccaac tgaagccaga
    10921 cacaaacaga aaattgtggc accggtgaaa cagactttga attttgacct tctcaagttg
    10981 gcgggagacg tcgagtccaa ccccggaccg gtgcatgtgg accccaagaa aaagcggaag
    11041 gtggacccta aaaagaaacg caaagtggat cctaagaaaa aaagaaaggt gcacgtgtcc
    11101 atggtgtcca agggcgagga actgttcacc ggcgtggtgc ccatcctggt ggaactggat
    11161 ggcgacgtga acggccacaa gttcagcgtg tccggcgagg gcgaaggcga cgccacatac
    11221 ggaaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ttggcctacc
    11281 ctcgtgacca cactgaccta cggcgtgcag tgcttcagca gataccccga ccatatgaag
    11341 cagcacgact tcttcaagag cgccatgccc gagggctacg tgcaggaaag aaccatcttc
    11401 tttaaggacg acggcaacta caagaccagg gccgaagtga agttcgaggg cgacaccctc
    11461 gtgaacagaa tcgagctgaa gggcatcgac ttcaaagagg acggcaacat cctgggccac
    11521 aagctggagt acaactacaa cagccacaac gtgtacatca tggccgacaa gcagaaaaac
    11581 ggcatcaaag tgaacttcaa gatccggcac aacatcgagg acggctccgt gcagctggcc
    11641 gaccactacc agcagaacac ccctatcggc gacggccctg tgctgctgcc tgacaaccac
    11701 tacctgagca cccagagcgc cctgagcaag gaccccaacg agaagaggga ccacatggtg
    11761 ctgctggaat tcgtgaccgc cgctggcatc accctgggca tggacgagct gtacaagcct
    11821 gcaggcgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccgggccg
    11881 tccaatttac tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc aacgagtgat
    11941 gaggttcgca agaacctgat ggacatgttc agggatcgcc aggcgttttc tgagcatacc
    12001 tggaaaatgc ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg
    12061 aaatggtttc ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc
    12121 ggtctggcag taaaaactat ccagcaacat ttgggccagc taaacatgct tcatcgtcgg
    12181 tctgggctgc cacgaccaag tgacagcaat gctgtttcac tggttatgcg gcggatccga
    12241 aaagaaaacg ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga acgcactgat
    12301 ttcgaccagg ttcgttcact catggaaaat agcgaccgct gccaggatat aaggaatctg
    12361 gcatttctgg ggattgctta taacaccctg ttacgtatag ccgaaattgc caggatcagg
    12421 gttaaagata tctcacgtac tgacggtggg agaatgttaa tccatattgg cagaacgaaa
    12481 acgctggtta gcaccgcagg tgtagagaag gcacttagcc tgggggtaac taaactggtc
    12541 gagcgatgga tttccgtctc tggtgtagct gatgatccga ataactacct gttttgccgg
    12601 gtcagaaaaa atggtgttgc cgcgccatct gccaccagcc agctatcaac tcgcgccctg
    12661 gaagggattt ttgaagcaac tcatcgattg atttacggcg ctaaggatga ctctggtcag
    12721 agatacctgg cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc
    12781 gctggagttt caataccgga gatcatgcaa gctggtggct ggaccaatgt aaatattgtc
    12841 atgaactata tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct gctggaagat
    12901 ggcgatctcg agccatctgc tggagacatg agagctgcca acctttggcc aagcccgctc
    12961 atgatcaaac gctctaagaa gaacagcctg gccttgtccc tgacggccga ccagatggtc
    13021 agtgccttgt tggatgctga gccccccata ctctattccg agtatgatcc taccagaccc
    13081 ttcagtgaag cttcgatgat gggcttactg accaacctgg cagacaggga gctggttcac
    13141 atgatcaact gggcgaagag ggtgccaggc tttgtggatt tgaccctcca tgatcaggtc
    13201 caccttctag aatgtgcctg gctagagatc ctgatgattg gtctcgtctg gcgctccatg
    13261 gagcacccag tgaagctact gtttgctcct aacttgctct tggacaggaa ccagggaaaa
    13321 tgtgtagagg gcatggtgga gatcttcgac atgctgctgg ctacatcatc tcggttccgc
    13381 atgatgaatc tgcagggaga ggagtttgtg tgcctcaaat ctattatttt gcttaattct
    13441 ggagtgtaca catttctgtc cagcaccctg aagtctctgg aagagaagga ccatatccac
    13501 cgagtcctgg acaagatcac agacactttg atccacctga tggccaaggc aggcctgacc
    13561 ctgcagcagc agcaccagcg gctggcccag ctcctcctca tcctctccca catcaggcac
    13621 atgagtaaca aaggcatgga gcatctgtac agcatgaagt gcaagaacgt ggtgcccctc
    13681 tatgacctgc tgctggaggc ggcggacgcc caccgcctac atgcgcccac tagccgtgga
    13741 ggggcatccg tggaggagac ggaccaaagc cacttggcca ctgcgggctc tacttcatcg
    13801 cattccttgc aaaagtatta catcacgggg gaggcagagg gtttccctgc cacagcttga
    13861 tgaagatctg agctcttaat taatgatcat aatcagccat atcacatctg tagaggtttt
    13921 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
    13981 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
    14041 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
    14101 caatgtatct tatcatgtct ggatccgttt aaactaactt taaataattg gcattattta
    14161 aagttacata tggcggccgc cctaaggctc gacctcgaaa ttctaccggg taggggaggc
    14221 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
    14281 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc
    14341 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc
    14401 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct
    14461 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
    14521 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
    14581 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcttccg gaggcccggc
    14641 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg
    14701 cctttcgacc tgcatccatc tagatctcga gcagctgaag cttaccatga ccgagtacaa
    14761 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
    14821 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccagatcgcc acatcgagcg
    14881 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
    14941 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
    15001 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
    15061 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
    15121 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
    15181 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
    15241 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
    15301 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgacgcc cgccccacga
    15361 cccgcagcgc ccgaccgaaa ggagcgcacg accccatgca tcgatgatat cagatccccg
    15421 ggatgcagaa attgatgatc tattaaacaa taaagatgtc cactaaaatg gaagtttttc
    15481 ctgtcatact ttgttaagaa gggtgagaac agagtaccta cattttgaat ggaaggattg
    15541 gagctacggg ggtgggggtg gggtgggatt agataaatgc ntgctcttta ctgaaggctc
    15601 tttactattg ctttatgata atgtttcata gttggatatc ataatttaaa caagcaaaac
    15661 caaattaagg gccagctcat tcctcccact catgatctat agatctatag atctctcgtg
    15721 ggatcattgt ttttctcttg attcccactt tgtggttcta agtactgtgg tttccaaatg
    15781 tgtcagtttc atagcctgaa gaacgagatc agcagcctct gttccacata cacttcattc
    15841 tcagtattgt tttgccaagt tctaattcca tcagaagctg gtcgagtaac tttaaataat
    15901 tggcattatt taaagttagg cgcgccataa cttcgtatag catacattat acgaagttat
    15961 gtcgagatat ttaaatatta gacccagctt tcttgtacaa agtggttgat atctctatag
    16021 tcgcagtagg cggtctggta agtaaattat ttggtaaagg taaagttgaa atgaacaaga
    16081 atgaggattg aaataaatga acaagagtaa aatcatcaga aatttaaagt tgtcgagagt
    16141 ggaagtcagg tttgattcca aattacaatg gtgttctccc aagaattacc agaactcagg
    16201 gtctcattgt tgggctgttc tgcttttcac ctcaaccatc tccttctgcc atcttagttg
    16261 tacaatatcc tgtgctattg attaccaata gtaattgtta tataccaaag tgctatcttc
    16321 acttagtttt cttaaagacc attctgccca gccaacaaat gagggatcta catcagactg
    16381 aaacatttac atcatatgat gtattttaca gctaatctgg aaatggagga accagaagaa
    16441 tcattgaaca ctctcaagct ttgttcccct gaagagttca caaggctttg cagagaaaag
    16501 acacaagaag tactctcctt tgttgttgaa atagagacac caaaccaatt gttcatacta
    16561 aagacaattt cttagatctc tgagcccaag agatggtgat ataactgcta tagattatga
    16621 tcttgattat aagcaagatc aatttttctt ttaatgcagt gaaaaaaatc atggtgttaa
    16681 ttattttatt tattttttct ctcctgttca tgagttatga aaattttttc tgcagtcatg
    16741 gtatcaaaat atctataaaa ctacacagtt gactaatagt tatctgtata ctggtttaaa
    16801 aaacacaaat actgaagaag gtttattgtt acagtatata gaatataata ttgatatatc
    16861 aaaactggta tcacaataat agtagtagat gcaatttttt aataattttc ttgacatgag
    16921 ctgatatctt tggattttat agttaagcta tcccttacat tctagggaac tttgactagg
    16981 tactaaggaa gagagtagta attagcctgg atttttttta agacatgtgt taactatccc
    17041 agttatattc ttgttctgtc tttagccctt gagaaggaca cagacagtat ctttcagtta
    17101 tagtttgagg gagaatgaag ctatataaga ccttaaggaa gcaatactta tgataatcaa
    17161 ttaggctttc aaacaactca agtaagccaa attcattgta tgtctaattt aaacagattt
    17221 acccaataaa ggaggccaat ggccgtacac gaaaggctct tatcatatgc aatacagagt
    17281 tcaaacatct ctcactgagg tatggggcta actttgacat cattggtatg aaaggccttc
    17341 ttgaagactt aggctacgat gtggtggtga aagaggagct tacagcagag gtaagagcca
    17401 ctgcaacatc ctatactggg aacacgaaga gttttaatca cagatgagca ttataatgtt
    17461 aaggagaaac tcaagtgaag gggtgtgtgt gtgagagaga gagagagaga gaaagagaga
    17521 gagagagaga gagagagaga gagagagaga agaagaagaa agaaagaaag aaagaaagaa
    17581 agaaagaaag aaagaaagaa agaaagaaag aaagaaagaa ggaaagaagg aaagaaggaa
    17641 agaaggaaag aaggaaagaa agaaagaaag aaagaaagaa agaaagaaag aaagaaagaa
    17701 agatcaaaat agtcaaatta ggtaaatgaa atccgtgcct tttaaagtta caaatatttc
    17761 atgtaccata aaaaagcgcc tggcagtgtg ggtttatgca gtatttttaa ttccaaagag
    17821 gagcaattga gactcagtgg ccgaacatga tgagatggcg caacgcaatt aatgataact
    17881 tcgtatagca tacattatac gaagttatgg tctgagctcg ccatcagttc agctataaga
    17941 aaaacttcaa agaatggagt atgtgtttat ctacataact aaaattgaat gaacattcat
    18001 tcaaactgat aggtgctaaa ggaaagggat gggagttgga aagtagagtg caataaaaga
    18061 taagaccatc agaaaacctt agtgaagtta atgaagatac tgaaatgcat gtactgagag
    18121 caaggggctc tgaaaaggtg tgagcagaga gactttcaag ttgagaggtg gtgcataagc
    18181 tacatctaga ctgcatggtg caagaagaaa gggaaagtat cagatttttt ttgtaaattt
    18241 ccaatgttga ttttaccgaa atgaggaggt taagatttat cctaactgtc atcccactaa
    18301 cgaagtcatc gatttttcca gggcatggag tcagagatga aagactttgc tgcactctca
    18361 gaacaccaga catcagacag cacattcctg gtgctaatgt ctcatggcac actgcatggc
    18421 atttgtggaa caatgcacag tgaaaaaact ccagatgtgc tacagtatga taccatctat
    18481 cagatattca acaattgcca ctgtccaggt ctacgagaca aacccaaagt catcattgtg
    18541 caggcctgca gaggtggtaa gttctttggt ggaaccacga actcccacgc tgctggctat
    18601 gcaggtgctg gcagggtaat gccacatggg ctttgtgtct agaagaatta actttaggct
    18661 taatttctat ttagttttca gaaattaatg gatctaatag gaaatgtgcc attacaaaaa
    18721 tttgcctggt tagttgaaag attttcatgt aacttttacc agtagaagtg attaagaact
    18781 tttccagaat ccccaagtca attgagacaa gtatacaaat attagagcaa aatggtttta
    18841 ttttctacta tacttgtggt ttttgtatta tcattttgaa atttatgttt gaaagtgaga
    18901 caatacaatt gttgtatctg agttcagtac cctactctta ctaatgaaca gaaaatagga
    18961 ccatacagaa ataaggtcag caatgaaaca ccagggttat agtaaatcaa gtgggaatca
    19021 ataatatcta cagaatattg taataaacat atacagaata tacatttaca attccagctt
    19081 tggggagaaa aggaatggta gattccagtg gaagacccca cagcccaatg gccaaatatc
    19141 acaatgtatt tgttaagttt ttggctagta tataaaatgt ttgtataata catttactac
    19201 tcaccaagat taccattcaa aatatccctc acactcatgt ttttttaagg acatagccaa
    19261 ttatacaatt agcctatgaa accatcaaca catgcctgga taaagaaaat gtctttatgc
    19321 ataattaagt actataaaac cataaaaaaa ataatttcct gaaatttgaa ggaaaataaa
    19381 aatggaactg tatatcttca tgttaagcaa gatgaatcaa tcacagaatg atgactgtcc
    19441 aatgttattt ttcagtttct tatacatgga aactaaataa aagtataaaa atgtgatctg
    19501 aagatataag agtgtttgta agtaaggaat tgaggtcgtg gagctgggga atatagcaag
    19561 aagggagagt gaaaagagat agagggagtc aaggcaaatg agagcagcat ggtcaaggga
    19621 aatgtgtcca tgtgtgaaag tacactatgt tacattcctg ctgcagtcac tgcacgaata
    19681 aaccagtata taaaaatcca atacttgaat gtgctcagat gtaattctgt cttttacttt
    19741 atcctgtaca ggtaatataa aataagtgct tcattatgat attctataca cttatacttg
    19801 acttgatcag tctataccct accaaccttt gctttctcta ttcctctctt gctcctccat
    19861 atcttttatt gaaacatttt cactttcacc tttgtgtgtc tctttagttt ttaatctatg
    19921 aaaagatgaa aactaatcta agactagatt taagtaaaat catctaaaat tgtcttccca
    19981 atctggctta ttttgcttaa catgatgacc ccaagtttaa cccatttaca ttcaaataat
    20041 aaaatttgga tctcttttca ttgcataatg ctttcttgtc catattaacc tcattgttct
    20101 tatacattca attattaatc ggtacctatg ctgatgtcat aatttggata tgatgaatag
    20161 taaaacaata catatgggtg tatacatatg tctattgtat attttatttt actgagtttc
    20221 actgtatatt tccaacttgt caggaactta cagtaatctc ctaccttagt ctcttgggag
    20281 ctgagattat agttgtgtgc tttcacatcc aaattgtagg atgcagatag gcctatatga
    20341 ctttgaattc atatagccat gggcctataa gtaactaaag tagaaataac ttttatataa
    20401 ccaaatggta taaaatgatg cagatataaa actcctctgc tgcttttagt aaacacgtcc
    20461 ttacaacttt atgactctgc ctttattttt tctcatcatg taacatttct caatgatcct
    20521 agagggaaag aaattctctc tgctctgtct ctgtctctgt cactctctgt gtgtgtgtat
    20581 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgcgc gcgcgcgcat gcgcgcgcgt
    20641 gcatatgctc ctacttacag tgcttgtatg tatatatatg tgaaactgat ctgagctctt
    20701 aaaatggctg aggtaggaaa ctggaaatct tactcagtgt gatcagtgct agattgcact
    20761 ttttttcccc cagggaactc tggagaaatg tggatcagag agtcttcaaa accccagttg
    20821 tgcagaggtg tagatctacc taggaatatg gaagctgatg ctgtcaagct gagccacgtg
    20881 gagaaggact tcattgcctt ctactctaca accccacgta tgtattttct aaaccatata
    20941 gctttgcagg tcaaaagaca tctttatata gcaatggtct aacacaaatt ttgagggtaa
    21001 caatttacat taattaaatc atatatttgt tttgagtaca tctactccta gacacaactt
    21061 tcaagactgg ccttttccta tagttaagga tttgtatagt gaaagatata tatattccag
    21121 tccacggatc caggggatcc tcttcacagc ttttaacata catgattaat ttgattcatt
    21181 tcttttaatc aaattatttt agcattctta cataaaaagt tatagatcaa agaattatca
    21241 gcctcttccc aaatattcag ccccaaggtc attttggcca acatcctgcc aaattctaga
    21301 tataccaatt ttatgacatg gtccactagg aatggggtat cctgttaaag aagttgtaga
    21361 aaacacattc acaggtctca aggtggggta tattgctgag cttttgatct ctgtatgtgt
    21421 ttcctaaagt gctttatgtc tactttttga atttgtatat ttttcatggc ttgttttaaa
    21481 aaactatttt gtgaaggcaa acttgataaa ctcaaacagc acaggtttaa tgtctacact
    21541 taatgaattt gaaaatatat gtaagacacc atcaggacag tgtatgacat ctacttacct
    21601 ctttcttctc aaagtatatc agtcttacgt attttccttg ctgagcatat tttgtatgaa
    21661 aacattatac atctaaccta ctgatcacac tttacagatc agtattatta ttggtgatgc
    21721 aaatgtaata catgtaaata caacagaccc tatgacttat tcatcttctg tacctcaaat
    21781 attggttctg tatagtattt tgttaattat atagtaaaaa agcaaagaac caatcaacaa
    21841 aacacaatga cataatctat agacataata aatgttatct atcagtagga agtgggttac
    21901 tgtacaaaa
//