LOCUS       187150#pL1L2_GT0_LF2A_nEGFPO_T2A_CreERT_puro#L3L4_pD223_DTA_T_spec        21343 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT0_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 187150
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000045583
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            8954..9111
                     /db_xref="ENSEMBL:ENSMUSE00000640565"
                     /rank="3"
                     /note="ENSMUSE00000640565"
     exon            8579..8672
                     /db_xref="ENSEMBL:ENSMUSE00000640566"
                     /rank="2"
                     /note="ENSMUSE00000640566"
     exon            8017..8248
                     /db_xref="ENSEMBL:ENSMUSE00000491682"
                     /rank="1"
                     /note="ENSMUSE00000491682"
     misc_feature    4275..10128
                     /note="5 arm"
     misc_feature    10156..10180
                     /note="B1 Gateway"
     misc_feature    10193..10240
                     /note="Frt"
     misc_feature    10241..10740
                     /note="mouse En2 intron"
     primer          complement(10309..10330)
                     /note="LRPCR LAR3_EN2"
     primer          complement(10205..10228)
                     /note="FRT3"
     primer          14392..14410
                     /note="seq primer NF"
     misc_feature    14452..14459
                     /note="RE sites"
     misc_feature    16252..16285
                     /note="loxP"
     misc_feature    complement(14460..14491)
                     /note="Rox"
     misc_feature    complement(16212..16243)
                     /note="Rox"
     misc_feature    14492..14513
                     /note="polylinker"
     CDS             15072..15668
                     /note="puromycin N-acetyl transferase"
     misc_feature    15187..15192
                     /note="Mutated RsrII site"
     intron          10741..11115
                     /note="mouse En2 intron"
     misc_feature    11113..11115
                     /note="Splice Acceptor"
     exon            11116..11155
                     /note="mouse En2 exon"
     primer          11016..11037
                     /note="seq primer FCHK2"
     misc_feature    11156..11278
                     /note="1Dleader"
     misc_feature    11279..11330
                     /note="F2A"
     misc_feature    11426..12142
                     /note="EGFP"
     misc_feature    11336..11425
                     /note="3X NLS"
     misc_feature    14208..14446
                     /note="SV40 polyadenylation site"
     misc_feature    13241..14185
                     /note="ERT2"
     promoter        14524..15035
                     /note="PGK promoter"
     misc_feature    15779..16211
                     /note="PGK pA"
     primer          14266..14289
                     /note="LRPCR RAF5"
     misc_feature    12152..12205
                     /note="T2A"
     misc_feature    12202..12202
                     /note=" A to G to kill RsRII site"
     misc_feature    12206..13231
                     /note="Cre"
     misc_feature    11131..11156
                     /note="FCHK lf2a_lacz_gt0"
     misc_feature    11131..11146
                     /note="FCHK QC prefix region"
     misc_feature    11147..11150
                     /note="FCHK QC critical region"
     misc_feature    11151..11156
                     /note="FCHK QC suffix region"
     misc_feature    16308..16328
                     /note="B2 Gateway"
     misc_feature    10129..16358
                     /note="Synthetic Cassette"
     exon            16729..16939
                     /db_xref="ENSEMBL:ENSMUSE00000119961"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 1 ENSMUSE00000119961"
     misc_feature    16359..17108
                     /note="Critical Region"
     misc_feature    17132..17165
                     /note="Downstream LoxP"
     misc_feature    17109..17188
                     /note="synthetic loxP region"
     exon            18758..18906
                     /db_xref="ENSEMBL:ENSMUSE00000119959"
                     /rank="5"
                     /note="ENSMUSE00000119959"
     misc_feature    17189..21343
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggatccact tggacaagga tatcaagagt atacaatgag gaaaggacac
     4321 atatgttgaa gcctccccgc ccagcatagc tgtagccatt tggttccttg cctgccaact
     4381 atttcatatt gttgccgtaa tatgtctgtc cgtcattggc acagaaaaat aacttgactc
     4441 ggagtagggg tcatgctgac cacataccca gttttgttcc atatgttata agctttgtta
     4501 atcttaagaa attccacaac tataagcttc ctgaaggccc catcaaatta aaggtcaata
     4561 tgaactgtta tgtctaaaat gacttgagtc agggttgacc cctgggcagc acttccaaca
     4621 tctgtgtatg agctccttgt gtttttgtgt ttttagcctt tataagctgt ccctgagaga
     4681 tgtccagctt tgagctgttg ccctggtcga tcggtcttgg ggatgtgcgt tcagtaagcc
     4741 attcctgctt agctgaaatt ggtgttcatg tggtttatgg gttgactcta gggctcccac
     4801 acaaggtcct ggtaatgcat ggtattggga agaccagatg tccacacaca caagaatgaa
     4861 cttgaagtct tatgctacat cattcaccaa aataaactaa aaatggatca ataacttaaa
     4921 tggaagctat taaactgtaa aactaccaga aaaatgggaa aagatttaca atattgacct
     4981 ttacaatgat ttcataactg tgacaacaaa aaacacaggc aacaaaagta gaaataagat
     5041 aagccatggg tgccgaatga aatttaaaag tttatgcaca gtaagagact caatcaatcc
     5101 tggaaaatag taaaccattg gatgctataa aatacacaac tgttctgctg ataatgcatt
     5161 gatcactaca atatatgaga aattctataa ggcagagcaa acaaaaacct aatatccaat
     5221 aggctcggga ttttgttgct gttatttttt tgagacagag tttctctgta tagtcctgac
     5281 tattctggaa cttaccccgt ggattaggct ggccttgaac tcagagatca actgttcctt
     5341 gcctcctgag tcctgggact aaaagcatgc agacccagct cagatttgag atttgaagag
     5401 acattttccc aaagaatgca catcaatgac caataattat acaaaaagat gatcaatatc
     5461 accgaaaaaa gcaaggaaag gctcagtgag ccttgacttc gcacctgttg tgtgtcgaga
     5521 tggcttttat gaagaaaggg gaaggaagtc ccattgcata ctaaatctag atgagcctca
     5581 actgaaataa gccagtcata gatggcaaat actgcaggat tctggcgggt tctttgttta
     5641 ttatttttgc ttttgagcca agagctctct acatagccct ggcttttctg gaactcatcc
     5701 gctatgttag acctggctgg cttcgaattc acagagatcc catgtcttcc tcccaagtgc
     5761 ttggattaaa ggtatgtgct accaatgcct gactaggtta ttgctatgta tcccaaactg
     5821 gttgggaact cttgtgattc ccaaccctct tgagtactga gagttctact tatccgaagt
     5881 ttctaaaaaa aaaagagcca cctttgagca gcagaaggga gagcaatgga tgccagaggt
     5941 tgagtcgggg gtggaattgt tcatctacac aggtgaaatt ccagatgtgc agggtggata
     6001 agctctagaa tcagcaggct agcaccatcc ctacaatctg tactgtgtcc ttgctgaaat
     6061 gcctgtcagg aactagaact catgccattt tctcactaca agaaaataaa tagcaaccaa
     6121 atcaaacaac aagtctctgt gaaagcaagc agttttattg atttgggcta tcgtaagcct
     6181 tattccatgg ttcgttttct ctctctcccc tccccctccc cctcccctcc cctcccctcc
     6241 cctctcttat cctctctctg cctcctcagt gctgccatta cagtcacatg ccactaagac
     6301 tagcttcatc ttgtttcttt aaaacctttt tttaaaacct atttatttgt ttctgggctt
     6361 gcttgtttgc acgcaagcat gcagagaaca tcgctgtccc tgtgctggca gaggtggggg
     6421 tagggtgggg gtagggtggg ggtaggtatg tatgtgaagg ggtgggccag aaaacaagat
     6481 ggtcttgata atgttcacaa gtaacttgga gctgaggtga cacaggtgcc aaacccccaa
     6541 agtggaaaag acggtattgg gctggacgta agtgtcgggg tgagccctgg gctggccaaa
     6601 gcctggtctg cgcggatgag agaggagggt agatcatggt atgccaggtg aacgtgcctg
     6661 aaaatctctc tagggagata gtcctggaga tggctggatg cctgtaaggc aggcaggcag
     6721 gcaggcaggc aggcgatgtt aagctattaa attctttcct ttcattcccc tcagtgttcc
     6781 ctatccctgt ctgaaattca gatgcctaaa cagtggagga tgcagagaag ggcgtggtgc
     6841 cagggctcat gaacttggtg tgttctttct gctctgtggt ctggtctaag gactgtttat
     6901 agcattagat caggaaaagg aagacccccc tggtgtaaac cctgagaccc acccctgcag
     6961 atctcagtgt tgtctcaaat aggaaaacaa ccaattctga catcctgaca gaatcctaac
     7021 agatttctgt ttctttcaag tgttttcgcc acaaatgtaa tcctttttct ttccttaaaa
     7081 ccttacgctg tggtcttgag ttcgagtttt ctagcagtgt ttactgcctg ttttgaaggg
     7141 gtggggaaaa ctggaggggc tgtctctata ctgtccccag ctctggacaa aagcagaagc
     7201 catggtcttc tgccaaatcg gcaaaagtga gcttacacac cactcctgtg tggattccct
     7261 ctcgttccac gggagctgtg tgtgagttgt ggaagcgtca gcacgtttcc ctcgggtgag
     7321 tcagagtaac gcaggtgctg ggaggaacgg cgtagagcga ctttctctca gtggttcatt
     7381 gaggccgaag tcctggctac agagcactgc ccaggtgtcc agagcaccgg aaacgtacag
     7441 atcagggaca gcccctctga catccagaag tctgcagatt cttgctcttc attaaggaaa
     7501 ggccgataag aagcgggatc gggataaagc aagggacagg aagcccggat tttatttctg
     7561 caatattcta taaaatcagg ctctgtcaaa gttttgaggc tgaaaccacc tcatatttct
     7621 tctggagggg aatgatagcc gtactgatac tacgctctgg ccattcccat cagagtccca
     7681 tccacagtta tatggctagg ccccatgagg aggtcatatg tgtctgacat gtctgtcatt
     7741 tgcttttata aaaccaaaag ttccccagat atgtataatg aatataatgt aacgttttac
     7801 aggccaagtg aaaggctctc cccgaatctc tctgcctctg cctcattctt tcggctatgc
     7861 gttcaggtca ctaggtcctc gtgactgaag tgtcactctt gatgaataat gcggggccat
     7921 gctgaaatat ttgtggctga gagctggacc ctcgtcattg ccacgcattt tgtcaccgag
     7981 gcagggtaac ccatggtaac taagctcaga tcagttaagg ggcagagagc ctcccctcta
     8041 ctgctctata aaagagaccc agcaaaggga ccctcgctgc tttgagctct cagtgtgggc
     8101 gaaggtgtgg gaagaaacgc ctaagatctc cgcagcagag cactccagct gcagacacaa
     8161 ggccagcatg tcaccgaact tcaaactcca atgtcacttc attctgatcc tcctgacagc
     8221 tctaagggga gagagccgct acctagaggt gagccatcct ttctcgagct taacaccttt
     8281 ctatcccttg agtatttagc cagggacagc agaccaactt ttgatccttt ctccctggtt
     8341 cacctagaca gctttctccg tgcccctgag catcctaact gcctgccttt ctctgggctg
     8401 ggtaccctaa tctggtggaa atagtcttcg actcacgcat aaactctgaa ctcaggagac
     8461 atgccccact gccccctgcc ccatttgctt ctctgagctg caaaaagggc tccctgctcc
     8521 tgcttcgctt tctttccctc cccctgaact tttccagacg atccactttc ctctccaggt
     8581 gcaagaagcc gcggtctacg accctctcct gctcttcagt gccaatctga agcgggacct
     8641 ggcagaggag cagccgtacc gacgggctct gcgtgagttg gggctgtcca gtgtgtgggt
     8701 gcccgggagg cgggaggcta agaacctagg acacaggctt gcaggatatc tggtcggggc
     8761 gggggggggg gggctggctg ggacccctgg gttgcagcat ccacgcggct ccttccaagc
     8821 cagagcgagg gaacccagtg cagcctaagc tgggagctcc aggttggggc gcgctgcgga
     8881 gccggggaat ggggcgcgct gagagcagct tcccaagaca gctgggaacc ccgggctcca
     8941 tgctctctcc cagggtgcct ggacatgctg agcctccctg gccagttcac cttcaccgca
     9001 gaccggccgc agctgcactg cgccgccttc ttcatcggcg aacccgagga attcatcacc
     9061 atccactatg acctggtctc catcgactgc caaggcgggg atttcctgaa ggtgaggggg
     9121 tccccactac tcgcggcccc agcccacgtt cccctaagcg cgtccatgtg ggggacaact
     9181 aaacctctaa gtgctcgagt ctcagaaact ccctgggaga gccccggagg ctggattcct
     9241 aggaagggtc tagcgccaaa cgctgcaggt ctagcgcccc tggacgtcga tggtttaatt
     9301 tcccttctgt gctctcccca actcctggga acttcctagc acttgaactt gcagtgtgag
     9361 acgctatgat gcaatctgcg gcgtacaaaa catggcaaaa ataattagca ggagctctcc
     9421 aggagggttc gcttgcgatc tgagatctga actcattttg taattccagg agcccgggct
     9481 gatggtggtt ttgaagtagt ggggcactgg ttgggagagg gatgctgcca ggaggtagag
     9541 cttgagagaa gcaagaagag gaagagaact cccagctcca cacatcccat ttcagccccc
     9601 agaggccaca ggatagagtt ctaccgctcc aagaaacatt cacattttag atctccaaaa
     9661 ccactggtga acctgtcaag cttgttagaa ataatttgca acccggcagt tggtaattgt
     9721 gctatgatgg actcattaaa atggagacct gcggtgattt ctgagaaccg ggtcaactga
     9781 ctttgaccct ttgtcccagg aggcaaagag gaagtcttac atggctaacc aggcagcact
     9841 gccagatttc agcatttaga aatattttaa aaagtagctt gaggtgatga tatatactaa
     9901 tatatatatt aattaatata taataatata ttaatgtgaa gagtgaggca ctggcaatga
     9961 cacctgctgc tctggatccc cccacgcccc tccccccatc actttgacac aatcaccctt
    10021 tgtatcccaa actcacggtg ctccccctta gcctctgtgg tactggggtt acaatcaccc
    10081 tccatcatat ccaggttggc tgttgttatt tattctttga gatctgttaa ggcgcataac
    10141 gataccacga tatcaacaag tttgtacaaa aaagcaggct ggcgccggaa ccgaagttcc
    10201 tattccgaag ttcctattct ctagaaagta taggaacttc gaaccctttc ccacaccacc
    10261 ctccacactt gccccaaaca ctgccaacta tgtaggagga aggggttggg actaacagaa
    10321 gaacccgttg tggggaagct gttgggaggg tcactttatg ttcttgccca aggtcagttg
    10381 ggtggcctgc ttctgatgag gtggtcccaa ggtctggggt agaaggtgag agggacaggc
    10441 caccaaggtc agcccccccc ccctatccca taggagccag gtccctctcc tggacaggaa
    10501 gactgaaggg gagatgccag agactcagtg aagcctgggg taccctattg gagtccttca
    10561 aggaaacaaa cttggcctca ccaggcctca gccttggctc ctcctgggaa ctctactgcc
    10621 cttgggatcc ccttgtagtt gtgggttaca taggaagggg gacgggattc cccttgactg
    10681 gctagcctac tcttttcttc agtcttctcc atctcctctc acctgtctct cgaccctttc
    10741 cctaggatag acttggaaaa agataagggg agaaaacaaa tgcaaacgag gccagaaaga
    10801 ttttggctgg gcattccttc cgctagcttt tattgggatc ccctagtttg tgataggcct
    10861 tttagctaca tctgccaatc catctcattt tcacacacac acacaccact ttccttctgg
    10921 tcagtgggca catgtccagc ctcaagttta tatcaccacc cccaatgccc aacacttgta
    10981 tggccttggg cgggtcatcc ccccccccac ccccagtatc tgcaacctca agctagcttg
    11041 ggtgcgttgg ttgtggataa gtagctagac tccagcaacc agtaacctct gccctttctc
    11101 ctccatgaca accaggctcc caggtcccga aaaccaaaga agaagaacgc agatctctag
    11161 agtcaccgag ttgctttacc ggatgaagag ggccgaaaca tactgtccaa ggcccttgct
    11221 ggcaatccac ccaactgaag ccagacacaa acagaaaatt gtggcaccgg tgaaacagac
    11281 tttgaatttt gaccttctca agttggcggg agacgtcgag tccaaccccg gaccggtgca
    11341 tgtggacccc aagaaaaagc ggaaggtgga ccctaaaaag aaacgcaaag tggatcctaa
    11401 gaaaaaaaga aaggtgcacg tgtccatggt gtccaagggc gaggaactgt tcaccggcgt
    11461 ggtgcccatc ctggtggaac tggatggcga cgtgaacggc cacaagttca gcgtgtccgg
    11521 cgagggcgaa ggcgacgcca catacggaaa gctgaccctg aagttcatct gcaccaccgg
    11581 caagctgccc gtgccttggc ctaccctcgt gaccacactg acctacggcg tgcagtgctt
    11641 cagcagatac cccgaccata tgaagcagca cgacttcttc aagagcgcca tgcccgaggg
    11701 ctacgtgcag gaaagaacca tcttctttaa ggacgacggc aactacaaga ccagggccga
    11761 agtgaagttc gagggcgaca ccctcgtgaa cagaatcgag ctgaagggca tcgacttcaa
    11821 agaggacggc aacatcctgg gccacaagct ggagtacaac tacaacagcc acaacgtgta
    11881 catcatggcc gacaagcaga aaaacggcat caaagtgaac ttcaagatcc ggcacaacat
    11941 cgaggacggc tccgtgcagc tggccgacca ctaccagcag aacaccccta tcggcgacgg
    12001 ccctgtgctg ctgcctgaca accactacct gagcacccag agcgccctga gcaaggaccc
    12061 caacgagaag agggaccaca tggtgctgct ggaattcgtg accgccgctg gcatcaccct
    12121 gggcatggac gagctgtaca agcctgcagg cgagggcaga ggaagtcttc taacatgcgg
    12181 tgacgtggag gagaatcccg ggccgtccaa tttactgacc gtacaccaaa atttgcctgc
    12241 attaccggtc gatgcaacga gtgatgaggt tcgcaagaac ctgatggaca tgttcaggga
    12301 tcgccaggcg ttttctgagc atacctggaa aatgcttctg tccgtttgcc ggtcgtgggc
    12361 ggcatggtgc aagttgaata accggaaatg gtttcccgca gaacctgaag atgttcgcga
    12421 ttatcttcta tatcttcagg cgcgcggtct ggcagtaaaa actatccagc aacatttggg
    12481 ccagctaaac atgcttcatc gtcggtctgg gctgccacga ccaagtgaca gcaatgctgt
    12541 ttcactggtt atgcggcgga tccgaaaaga aaacgttgat gccggtgaac gtgcaaaaca
    12601 ggctctagcg ttcgaacgca ctgatttcga ccaggttcgt tcactcatgg aaaatagcga
    12661 ccgctgccag gatataagga atctggcatt tctggggatt gcttataaca ccctgttacg
    12721 tatagccgaa attgccagga tcagggttaa agatatctca cgtactgacg gtgggagaat
    12781 gttaatccat attggcagaa cgaaaacgct ggttagcacc gcaggtgtag agaaggcact
    12841 tagcctgggg gtaactaaac tggtcgagcg atggatttcc gtctctggtg tagctgatga
    12901 tccgaataac tacctgtttt gccgggtcag aaaaaatggt gttgccgcgc catctgccac
    12961 cagccagcta tcaactcgcg ccctggaagg gatttttgaa gcaactcatc gattgattta
    13021 cggcgctaag gatgactctg gtcagagata cctggcctgg tctggacaca gtgcccgtgt
    13081 cggagccgcg cgagatatgg cccgcgctgg agtttcaata ccggagatca tgcaagctgg
    13141 tggctggacc aatgtaaata ttgtcatgaa ctatatccgt aacctggata gtgaaacagg
    13201 ggcaatggtg cgcctgctgg aagatggcga tctcgagcca tctgctggag acatgagagc
    13261 tgccaacctt tggccaagcc cgctcatgat caaacgctct aagaagaaca gcctggcctt
    13321 gtccctgacg gccgaccaga tggtcagtgc cttgttggat gctgagcccc ccatactcta
    13381 ttccgagtat gatcctacca gacccttcag tgaagcttcg atgatgggct tactgaccaa
    13441 cctggcagac agggagctgg ttcacatgat caactgggcg aagagggtgc caggctttgt
    13501 ggatttgacc ctccatgatc aggtccacct tctagaatgt gcctggctag agatcctgat
    13561 gattggtctc gtctggcgct ccatggagca cccagtgaag ctactgtttg ctcctaactt
    13621 gctcttggac aggaaccagg gaaaatgtgt agagggcatg gtggagatct tcgacatgct
    13681 gctggctaca tcatctcggt tccgcatgat gaatctgcag ggagaggagt ttgtgtgcct
    13741 caaatctatt attttgctta attctggagt gtacacattt ctgtccagca ccctgaagtc
    13801 tctggaagag aaggaccata tccaccgagt cctggacaag atcacagaca ctttgatcca
    13861 cctgatggcc aaggcaggcc tgaccctgca gcagcagcac cagcggctgg cccagctcct
    13921 cctcatcctc tcccacatca ggcacatgag taacaaaggc atggagcatc tgtacagcat
    13981 gaagtgcaag aacgtggtgc ccctctatga cctgctgctg gaggcggcgg acgcccaccg
    14041 cctacatgcg cccactagcc gtggaggggc atccgtggag gagacggacc aaagccactt
    14101 ggccactgcg ggctctactt catcgcattc cttgcaaaag tattacatca cgggggaggc
    14161 agagggtttc cctgccacag cttgatgaag atctgagctc ttaattaatg atcataatca
    14221 gccatatcac atctgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
    14281 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
    14341 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
    14401 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cgtttaaact
    14461 aactttaaat aattggcatt atttaaagtt acatatggcg gccgccctaa ggctcgacct
    14521 cgaaattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag
    14581 cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat
    14641 ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc
    14701 ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa
    14761 tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc
    14821 gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga
    14881 ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg
    14941 cccgaaggtc ttccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc
    15001 tgttctcctc ttcctcatct ccgggccttt cgacctgcat ccatctagat ctcgagcagc
    15061 tgaagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
    15121 ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
    15181 tcgatccaga tcgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
    15241 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
    15301 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
    15361 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
    15421 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
    15481 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
    15541 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
    15601 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
    15661 ccggtgcctg acgcccgccc cacgacccgc agcgcccgac cgaaaggagc gcacgacccc
    15721 atgcatcgat gatatcagat ccccgggatg cagaaattga tgatctatta aacaataaag
    15781 atgtccacta aaatggaagt ttttcctgtc atactttgtt aagaagggtg agaacagagt
    15841 acctacattt tgaatggaag gattggagct acgggggtgg gggtggggtg ggattagata
    15901 aatgcntgct ctttactgaa ggctctttac tattgcttta tgataatgtt tcatagttgg
    15961 atatcataat ttaaacaagc aaaaccaaat taagggccag ctcattcctc ccactcatga
    16021 tctatagatc tatagatctc tcgtgggatc attgtttttc tcttgattcc cactttgtgg
    16081 ttctaagtac tgtggtttcc aaatgtgtca gtttcatagc ctgaagaacg agatcagcag
    16141 cctctgttcc acatacactt cattctcagt attgttttgc caagttctaa ttccatcaga
    16201 agctggtcga gtaactttaa ataattggca ttatttaaag ttaggcgcgc cataacttcg
    16261 tatagcatac attatacgaa gttatgtcga gatatttaaa tattagaccc agctttcttg
    16321 tacaaagtgg ttgatatctc tatagtcgca gtaggcggag ttgatctcat gtggaaacga
    16381 atgaataaaa tcagcgtcgt ctgtagttaa tgcccttcta ccaaactagg tgataaggta
    16441 taaaaaagtg tgccaactta gcagcctgaa ggcttatgca gaaaagatag tgcttgcaac
    16501 caaactgcac agtcttaata aataccactg aaaagcaagc aggctgtgag tcccggctgt
    16561 ggcttcccca cttaaaaacc gaaggttgtt aacgtcccct ctttacaaca cattgtgatt
    16621 ttagaagctg gtgaaagcga ctgtgtatgt ctgtactgtt gttgttgttg ttgttattat
    16681 tattattatt attattatta ttattattat tattattatc atttgaaggt atttgatggt
    16741 tggatcctaa agggagagaa attcccaagt tctcaggatc accctctgcc cactatgaag
    16801 aggtacacag atttctgtga gagtggtctt accagaagga gcatcaggtc ttcccagaat
    16861 gtggccatgg tcttcttccg ggtccacgaa ccaggaaatg gattcacgat aaccataaag
    16921 acagatccca acctcttccg taagtaaaca cgagcaacag acaggactca ggtggggagg
    16981 cggtcacttc tgctttgtaa agattgtatt acctcacaga cttaagccca ggaatggtat
    17041 gggctcttta atcctggtta tgaaaattaa accacagttt ctgattgtat ttcccaggct
    17101 aggtaataga gatggcgcaa cgcaattaat gataacttcg tatagcatac attatacgaa
    17161 gttatggtct gagctcgcca tcagttcaat tcgggataag cttgtgggat catttggtgc
    17221 ctgagctttg taaacaaagt ctgagacagg tgggcagatg ttgtcttgtc ccctaaggag
    17281 ggagtgaacc agtgcccctc catcacctcc ttccacgtta ccttctacca caaagccacc
    17341 accccagctt gtgtcatttc tgacagagag aaacatagat taggctggaa aactagatag
    17401 ccagcctgaa agagcgtgat tctgagcaga acgagttcgt acatcggctc atttcctcat
    17461 taaggggaaa agctgagaac tagggatgta gttaagtggt aaaccgtttg tctaagaatg
    17521 cttgaagcaa aacttgggtt caaactctag cactagggag ggaagaagct agggagaaga
    17581 gagggaaaca ggaaggagag ggaaagaaat tctgccctga aaagctgagg ctatctgaga
    17641 aacagaatgt tgggggttca tttctgtggg catttgcttg gtaaaaaaat cagaagatgt
    17701 ctttttaaac tgtcaaggaa tttcacttat tcctgaattt aagagtcatt aaaaaatact
    17761 tatttctgaa attaagagta attaaaaaca taaggcggtg ggtcagcaga aaggccaatc
    17821 ccatgcctct gtttcacaga cagaagaata actcagttgt aagattagac ttgaaataaa
    17881 aagaaaaatt tcagaaaatc ttaagactat cacgatttaa aggttaaagc tgggcatggt
    17941 gtcccacacc tttaacccca gcacttaggg gcaaaggcgg atagatctct gagtttgaag
    18001 ccagcctggt ctacagagtg agttctagga cagccagggc tacacagaga aaccctgtct
    18061 caaagcaaca acaacaataa tacatataaa aatttaaaaa aagaccactt agtcaacttt
    18121 acattactct aaacccagca aatatgtatt aggtataaaa ctgttgtcta ttttattctc
    18181 cacttcttct gtcagtatca tgaaatcttt tgaataaaat tagacatggt ttttattgtt
    18241 taacaaacaa caccgtggct tcaaattcta tgggtgaggt tctgacttcg tggttgagcc
    18301 ttgttctccc agttcggatc tagaatcaac tcaacagaag tattcctggt acccccaggt
    18361 atgttgtctg actgataaag acttcctacc ttaggattgt tataaacaga caaaggtgag
    18421 aagccctctt gtggctggca cttccggaca atattcacat gtcactgagt tctagtcccc
    18481 tgctccttcc tctccccaga ttgtcatggt gatatatacc ctctccctgg gcccaacacc
    18541 taaatagaga ggtccagccc ttgaatgttg gtaatctaca aatatgttta gcaccattca
    18601 aagcaatcct gggacacatg tggccattgg gctccaggtt ggatatacct actagaccat
    18661 gaactgaaat ctagagcaaa ggcataaata atttatttta aaaagtccag cactctaaat
    18721 aatgtatttg tatctttttt tttttttacc tcctaagctt gcaatgtcat ctctcagact
    18781 ccgagtggaa ggtttacttt agtggttcca taccagcacc aaaactgcag cttctccatc
    18841 atctatccag tggcaatcaa aatctctgat cttaccctcg gacacttgca tggccttcag
    18901 ttaaaggtca gtggcttgct tgctccttgg ccatttggta aggccactgt tgtttttgtt
    18961 gttgtttgtt tgtttgtttg tttggtttgt tttgttttat cagtgacaaa taacccatcc
    19021 tgtcaggaag catgtggccc ctgactttta agtcttctgc acgatgccct ggcttcctca
    19081 atgtctattt cagtcacgct gcaggcaaac actcaggtgc ccattctgac tactcaagga
    19141 tttctcttgt gacataggaa atgctttcag ttggtaaagc tgcagcatgt aattacaatc
    19201 gaacactctt gtactttcct cttttacaaa aatcctctcc tagctgagcc tcctctgagt
    19261 catcgacaca gctgctctcc tgattattgg aattttcttt tgataagaag gaagtcactc
    19321 tcattgacag tactaggcta agaaagaatg ctaacggtgg cataaaatat gaaattggat
    19381 ttttttctat tgcaaaaagc agtaaagttt tgaagagcag gttatatgac atcttgctta
    19441 atatatatgt gcacacacag atatacatat acacatatac gtatatacat atatgtgtat
    19501 atgtatttat atatgtgtat atatgtatgt gtgtgtctaa tatatacata tacatatata
    19561 tatacatata tatatatgta tatatatata tgctgtgaaa tttcaaccaa cttgctttca
    19621 tatcagcttc cgtgtgtgcc ttacaatgaa tgctggcttc acagcatccc tctgcaaaga
    19681 caatggctgc tccatgagtc accttcattt ccttttcctt gttcatcggc ctaagttaag
    19741 ataatgcgtg cacttggtgg tatcagaggc agccggtgtt cccaaacata cagatctttg
    19801 tgaaaactaa ataaggaaca agaccgagag aaagaattga tatctaactt cttccagccc
    19861 ccacacccac gtgtccctgc ttcttcttct ttgttgccct tggactttga ttgaggagcg
    19921 atcctttgat aagaatgtct gagtgctttg gatccagcct gacagtgttc gtaagccatt
    19981 ttattcttga tgaaagatct agaatctggt gggatacatg gtaacatatt aggaaatctg
    20041 tgattttggt atgaaagctc taagaatact tttatatttc ccttaatatt ttttgtgcct
    20101 actgtgttct aggcatctat tcctttgaaa acctattttt tgacacctgg gtattgaaag
    20161 ctgggtatgg tggtttatac ttgtaatccc agtactgaga gactgagata ggagaacttc
    20221 ttgtgacagg cttgcatgac cttcccttta ctagtactgc caaacaacaa caataataat
    20281 ttcctttgct cagggactat tcttggttct agaaataagc aatgaacaag gcaggtcctg
    20341 ctcacacagg gagcctattt gaagtgggat ggcacaggca aaattatcat gaatttcaaa
    20401 tcatggtaag tctgtccaga aaatgcagca gagtggattg atagacagac aggagctgga
    20461 aagtgggtag acggaactgc ctgagacagt gtggtcagga aaggaaactt agattagaag
    20521 aatcagcaac cataggaggc aggaggagtg gatggaacag tgctgccctg gcagaaacag
    20581 gcttactgga tcctagacac aggaagaagc aagctcgaga gggagttggg cagagccccg
    20641 tgtgctgccc acccggagaa ggttgtgtgc agcaggagta gtgggaaagc ctttggggtt
    20701 gggtgggaaa ggacctgatg tgtgctgtcc tgaaggtcat tctggcttcc acctgacgaa
    20761 tgggctgggg gacatgctgc gagatgcctc acgttccccg tgagaagact ctatggttgt
    20821 gctagtgaac cactgaagat catcccataa gcacctcagc ttccatgtgt gctgggggtg
    20881 agacttcaga cattcagaac gaacagcagg gatttggctt ccagttcaac aagctggagt
    20941 gagcctttgg cacagggtca caatctagca agagggggta ggagcttaac gcttcaacaa
    21001 agacacgcgg gagctgctga ggccccggaa ccccagacag gtcctcatgc agctcacttt
    21061 ctgccactgt ttacaagaac acctgagagg attccaaaga ggagagagat ggcatcactc
    21121 accaagtgga aacttccgtc tatctactca tgggaagggc aattattgtt cttccactcc
    21181 taaatagctt gtttgaatgt ctgttcacaa acttgtgcac agtgggccta attaaatctt
    21241 tatttcatca tgtaatgaaa ttacgtttga agtacctaag catacagtca tgctcatagt
    21301 aagttcttta aacataagca gaccctgttg tctccattcc tta
//