LOCUS       48056#pL1L2_GTK_nEGFPO_T2A_CreERT_puro#L3L4_pD223_DTA_T_spec        22677 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GTK_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 48056
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000089188
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            7458..7608
                     /db_xref="ENSEMBL:ENSMUSE00000556757"
                     /rank="1"
                     /note="ENSMUSE00000556757"
     exon            7765..7824
                     /db_xref="ENSEMBL:ENSMUSE00001014391"
                     /rank="2"
                     /note="ENSMUSE00001014391"
     misc_feature    4275..8534
                     /note="5 arm"
     misc_feature    8562..8586
                     /note="B1 Gateway"
     misc_feature    8599..8646
                     /note="Frt"
     misc_feature    8647..9147
                     /note="mouse En2 intron"
     primer          complement(8715..8736)
                     /note="LRPCR LAR3_EN2"
     primer          complement(8611..8634)
                     /note="FRT3"
     primer          12650..12668
                     /note="seq primer NF"
     misc_feature    12710..12717
                     /note="RE sites"
     misc_feature    14510..14543
                     /note="loxP"
     misc_feature    complement(12718..12749)
                     /note="Rox"
     misc_feature    complement(14470..14501)
                     /note="Rox"
     misc_feature    12750..12771
                     /note="polylinker"
     CDS             13330..13926
                     /note="puromycin N-acetyl transferase"
     misc_feature    13445..13450
                     /note="Mutated RsrII site"
     misc_feature    9684..10400
                     /note="EGFP"
     misc_feature    9594..9683
                     /note="3X NLS"
     misc_feature    12466..12704
                     /note="SV40 polyadenylation site"
     misc_feature    11499..12443
                     /note="ERT2"
     promoter        12782..13293
                     /note="PGK promoter"
     misc_feature    14037..14469
                     /note="PGK pA"
     primer          12524..12547
                     /note="LRPCR RAF5"
     misc_feature    10410..10463
                     /note="T2A"
     misc_feature    10460..10460
                     /note="A to G to kill RsRII site"
     misc_feature    10464..11489
                     /note="Cre"
     misc_feature    9422..9443
                     /note="seq primer FCHK2"
     misc_feature    9148..9584
                     /note="mouse En2 exon"
     misc_feature    9536..9604
                     /note="FCHK cre_ert_gtk"
     misc_feature    9536..9571
                     /note="FCHK QC prefix region"
     misc_feature    9572..9584
                     /note="FCHK QC critical region"
     misc_feature    9585..9604
                     /note="FCHK QC suffix region"
     misc_feature    14566..14586
                     /note="B2 Gateway"
     misc_feature    8535..14616
                     /note="Synthetic Cassette"
     exon            14982..18053
                     /db_xref="ENSEMBL:ENSMUSE00000556750"
                     /type="targeted"
                     /rank="3"
                     /note="target exon 1 ENSMUSE00000556750"
     misc_feature    14617..18247
                     /note="Critical Region"
     misc_feature    18271..18304
                     /note="Downstream LoxP"
     misc_feature    18248..18327
                     /note="synthetic loxP region"
     misc_feature    18328..22677
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggatctaat ccttacctct atactgagca cactttatgt atccattttt
     4321 tttccctcct tggaagcata ctctgtacag ggagaaaagt agaaaagggg gactaatttt
     4381 tctcatgaac tttgtataat aatgaaagca ttattggaga gaaaactccc aagaattttt
     4441 taaaccctgg ttcttgggct agagagatgg tttagtggtt aggagcattt gctgctcttg
     4501 cagaggaccc tggttttgtt ctcagcatcc acatggcagc tcagaaacct ttgttaactc
     4561 tggttccagg ggatctggtg ccctcttctg acctctgcag gcattgcatg cacatagtgc
     4621 acttacatct acaaaggcaa aacactcatg cacacaaagt aaaaataaat aaatcttaaa
     4681 caaaaacctt tggttccttt caaacccact gttaagtaat cttacaggaa agaataggtt
     4741 ctcagaaaaa ttaatgtgat ttattactaa atctgctagc ttagattaat ttgttcttaa
     4801 gagctacata atttattcaa atgacccaaa gcttttaaag tcaatcttca agttaaaaat
     4861 aaaaatcttt atccagtccc caggagagtg tttttttcct gtgttgcatt gcttcccatg
     4921 actgacattt atttgactct caagtgtatt tacattgttg gcttaactgg gaggtcattg
     4981 ctgaatcaat aatgggctgt attttttttt ttcttttttg ctttgagtgg tgtcaatatt
     5041 cttgctattt cccctttctt ctacccccct aattccttga agttacaaat tttctctttg
     5101 gcctgtgaac atttttattt taaaattatg aagaaacaaa gtataggtct ttttaatctt
     5161 acgggaagtg tgtgaatctt taaagtttgt ttacgtcaaa attagctgta gttaagaatg
     5221 aaagctctag acaaggtccc ctgtttctaa ggagtgttca aggctgggtc tcttggaggg
     5281 cagcctgcag ggattttttt cctctctaaa gtttatagct cactgcatga tcatttcttt
     5341 gcaagtgggc acaaagagga gcttgaacta gaccaggata ggaacctaac ccattgtgac
     5401 cactcagaag ttctggcctg gaaaattccc tggatgtggc aaaaataaac atttaaaatg
     5461 aaggcagaaa ccaggcttaa gcaaagaatg aaactcattc tcctttcaaa tctcctgccg
     5521 ataaacatat gtgcccagtc ttttgtttcc tagacatcag gtttccatta tttaaacaga
     5581 gcttctacct ggatctgtct agagcatgag gcagaaatag tttgagattt ttacaaactg
     5641 gtgtatatgt atattaggtg catttttgcc aatgttcaag ttcatttaat caaaacaagt
     5701 gctacaatgg tgtccacaag cctttggagc ttactgaaat gttcaggcaa tgtgaagcaa
     5761 cacttacttg ttctatatgc tcatgtgtta caggatttac tttaatgtca gtggacggac
     5821 agatagcaca agctgtctgc tgcttcttga gacatttgta attttgtcct cttatgtatc
     5881 acttctcttt ccttgtttta ctgaacttta tacttatgta cctaaatact aaggacaaag
     5941 atatatattc ctctgcttgc agcttgggat acactaatgc tcactatggt actaccaatg
     6001 caccatgccc tctttccagg atccatggga atccttgtaa ttgcacacct ctttttggtg
     6061 ttgtggatta cctggttatc gacacaaact gcagaaaatt tcacaattcc atatttctct
     6121 tctctgatct ccattttgct aatacattta agaacacttt cccttgcata gcaatccttc
     6181 caactttctt ctttggcgtt tttgcaatag cttattaact gtcagcagtg actattgtct
     6241 gcaaatgccg tatgtattcc ttttgctcag agaatggtga gacaaaagaa tcagctagca
     6301 gggtagagat gaaaaaatat gagccctggc aagagtttac ttttgttgaa attcacatgt
     6361 cctcataccc gtgagaccct agggaattta tttaaattct gtcacccttt gtttcctgat
     6421 cagtaaatcg gcaataatat taatagtttt taagggcatt tgctgtgtgt taagcatcat
     6481 cttaagtact ctaaatagga tagttcattt actttttaat aacttcatga tatgagtaat
     6541 tagtatcccc atttctcaga tgacacgaag aaggaaagtc atgtgactcc actcagagaa
     6601 gtgagatgcg gcagaaatta gattgtgaag cccttcagag gccattcctt actttttgct
     6661 atggcgctct ctgttgatct attgaggaca cagtaacgta gcacagtgtt aggtgcgaag
     6721 catttgctat cagctggtac tgtacaatgc catcacctct tatggactgt atccattttt
     6781 ttcttgctgt tgctggatgg tatgaaggtg atcattcttc cagagaaaag gaaaaacagt
     6841 cataaccata ccagcagccg caatagcagc aatctataat agcagcaatc tataatagca
     6901 gcctaatcgt tttctacctt gtctgctata tatttgagct acagttgtga cagtcacatg
     6961 aaaaaaaaaa ggcactagat gttgattttt tttttgtcac aacaaaattt tatccttctt
     7021 atccccaacc ttctagcagc agggcaacca accaaaatgg ttcaaagcaa ggtaaaaatt
     7081 taaaagttgt agacctcaag tagaattgct accattcttt agtaaatagg agtaaaaatt
     7141 caaaatctgc taaaagctgt aaaagatgct tgtgaacaaa gcctgctact agaatcaact
     7201 ctctggtctt cctagtcctt aacttacttt agagtgtcag gatcagaaaa agagaaagag
     7261 aaaattctgc taaaaatgat attcttctct taaagaaagc cacggagttt aaaaatctct
     7321 aacacatttg tggaaacgtt attatttttt tgtttgagat ttatttgaat gagctgttat
     7381 gattggagac atgagaactt cagattaatg ttttgcagcc agaagaaaag ccctctggaa
     7441 agctggcaag tgttcataag tcagctccag aattctgtag gttgaaggct ccccagtgga
     7501 cagagcgaat atataagaag gaaaccagag atctggtgca gttacatctc agaggctggc
     7561 gatggaggga gctcggaact gaaagcttac ttcagcctgc attttaaggt aaggcagaac
     7621 aatttacaca tgcttgacta gggaagggga acatacagca gtaaaaatgt ttctgtgtgt
     7681 gcgtgtatga atgttttctt tttcctgtat tttaactgca ttttgcagaa tgccttaatt
     7741 ctttaactgc catttctctt gcaggagtgc atgcgggagc tgagtaagct gatttatgat
     7801 aactgcttta aacactggca actagtaagt ctgtagtggg atttattttt tattcttgca
     7861 tgttaatttg gaaggtcata gaaaagaatg ggcctttgac agaaatggta gtttgccgtt
     7921 tctgaggaag ttctctagtg gttacatttt aaattgaata tgtgattgta tgtactttga
     7981 aatgatctgg tgtaatagtt ttgctctctg tgtgtttatc tttagttttc ttttgtttca
     8041 aagtaatgtt gctagttgaa agaaaacagc agccttccca cctgaacgct gttgttaatg
     8101 ctggaaagta atgactattc agttctgtta aattgaaaag atagcaaaga aaaagcagta
     8161 aaactatatc ataattagtc actaatgtct aattctgatt taattgagcc tccaaaccca
     8221 tctctgtctg cccttgactc gggtttagtg tttttgtttt ctttatcttc tatttaaact
     8281 gaaaatagat gtttcttatg aaacagatca gattcaaata tccatattga aaactggcat
     8341 tgactattaa aatttttagg taaaagcttt aagaaattca tctatatctt tttgttgttg
     8401 ttaaagcaaa gggaaaataa atacattatt tttaattagc aaaacccaac cctgactcta
     8461 tcctaatatg caccttctgc atattttttc cagctctcag tacacttacc tatttttccc
     8521 tatggcttat gttaaaggcg cataacgata ccacgatatc aacaagtttg tacaaaaaag
     8581 caggctggcg ccggaaccga agttcctatt ccgaagttcc tattctctag aaagtatagg
     8641 aacttcgaac cctttcccac accaccctcc acacttgccc caaacactgc caactatgta
     8701 ggaggaaggg gttgggacta acagaagaac ccgttgtggg gaagctgttg ggagggtcac
     8761 tttatgttct tgcccaaggt cagttgggtg gcctgcttct gatgaggtgg tcccaaggtc
     8821 tggggtagaa ggtgagaggg acaggccacc aaggtcagcc ccccccccct atcccatagg
     8881 agccaggtcc ctctcctgga caggaagact gaaggggaga tgccagagac tcagtgaagc
     8941 ctggggtacc ctattggagt ccttcaagga aacaaacttg gcctcaccag gcctcagcct
     9001 tggctcctcc tgggaactct actgcccttg ggatcccctt gtagttgtgg gttacatagg
     9061 aagggggacg ggattcccct tgactggcta gcctactctt ttcttcagtc ttctccatct
     9121 cctctcacct gtctctcgac cctttcccta ggatagactt ggaaaaagat aaggggagaa
     9181 aacaaatgca aacgaggcca gaaagatttt ggctgggcat tccttccgct agcttttatt
     9241 gggatcccct agtttgtgat aggcctttta gctacatctg ccaatccatc tcattttcac
     9301 acacacacac accactttcc ttctggtcag tgggcacatg tccagcctca agtttatatc
     9361 accaccccca atgcccaaca cttgtatggc cttgggcggg tcatcccccc ccccaccccc
     9421 agtatctgca acctcaagct agcttgggtg cgttggttgt ggataagtag ctagactcca
     9481 gcaaccagta acctctgccc tttctcctcc atgacaacca ggtcccaggt cccgaaaacc
     9541 aaagaagaag aacgcagatc ataactgact aggaggccac catgggcgga ccggtgcatg
     9601 tggaccccaa gaaaaagcgg aaggtggacc ctaaaaagaa acgcaaagtg gatcctaaga
     9661 aaaaaagaaa ggtgcacgtg tccatggtgt ccaagggcga ggaactgttc accggcgtgg
     9721 tgcccatcct ggtggaactg gatggcgacg tgaacggcca caagttcagc gtgtccggcg
     9781 agggcgaagg cgacgccaca tacggaaagc tgaccctgaa gttcatctgc accaccggca
     9841 agctgcccgt gccttggcct accctcgtga ccacactgac ctacggcgtg cagtgcttca
     9901 gcagataccc cgaccatatg aagcagcacg acttcttcaa gagcgccatg cccgagggct
     9961 acgtgcagga aagaaccatc ttctttaagg acgacggcaa ctacaagacc agggccgaag
    10021 tgaagttcga gggcgacacc ctcgtgaaca gaatcgagct gaagggcatc gacttcaaag
    10081 aggacggcaa catcctgggc cacaagctgg agtacaacta caacagccac aacgtgtaca
    10141 tcatggccga caagcagaaa aacggcatca aagtgaactt caagatccgg cacaacatcg
    10201 aggacggctc cgtgcagctg gccgaccact accagcagaa cacccctatc ggcgacggcc
    10261 ctgtgctgct gcctgacaac cactacctga gcacccagag cgccctgagc aaggacccca
    10321 acgagaagag ggaccacatg gtgctgctgg aattcgtgac cgccgctggc atcaccctgg
    10381 gcatggacga gctgtacaag cctgcaggcg agggcagagg aagtcttcta acatgcggtg
    10441 acgtggagga gaatcccggg ccgtccaatt tactgaccgt acaccaaaat ttgcctgcat
    10501 taccggtcga tgcaacgagt gatgaggttc gcaagaacct gatggacatg ttcagggatc
    10561 gccaggcgtt ttctgagcat acctggaaaa tgcttctgtc cgtttgccgg tcgtgggcgg
    10621 catggtgcaa gttgaataac cggaaatggt ttcccgcaga acctgaagat gttcgcgatt
    10681 atcttctata tcttcaggcg cgcggtctgg cagtaaaaac tatccagcaa catttgggcc
    10741 agctaaacat gcttcatcgt cggtctgggc tgccacgacc aagtgacagc aatgctgttt
    10801 cactggttat gcggcggatc cgaaaagaaa acgttgatgc cggtgaacgt gcaaaacagg
    10861 ctctagcgtt cgaacgcact gatttcgacc aggttcgttc actcatggaa aatagcgacc
    10921 gctgccagga tataaggaat ctggcatttc tggggattgc ttataacacc ctgttacgta
    10981 tagccgaaat tgccaggatc agggttaaag atatctcacg tactgacggt gggagaatgt
    11041 taatccatat tggcagaacg aaaacgctgg ttagcaccgc aggtgtagag aaggcactta
    11101 gcctgggggt aactaaactg gtcgagcgat ggatttccgt ctctggtgta gctgatgatc
    11161 cgaataacta cctgttttgc cgggtcagaa aaaatggtgt tgccgcgcca tctgccacca
    11221 gccagctatc aactcgcgcc ctggaaggga tttttgaagc aactcatcga ttgatttacg
    11281 gcgctaagga tgactctggt cagagatacc tggcctggtc tggacacagt gcccgtgtcg
    11341 gagccgcgcg agatatggcc cgcgctggag tttcaatacc ggagatcatg caagctggtg
    11401 gctggaccaa tgtaaatatt gtcatgaact atatccgtaa cctggatagt gaaacagggg
    11461 caatggtgcg cctgctggaa gatggcgatc tcgagccatc tgctggagac atgagagctg
    11521 ccaacctttg gccaagcccg ctcatgatca aacgctctaa gaagaacagc ctggccttgt
    11581 ccctgacggc cgaccagatg gtcagtgcct tgttggatgc tgagcccccc atactctatt
    11641 ccgagtatga tcctaccaga cccttcagtg aagcttcgat gatgggctta ctgaccaacc
    11701 tggcagacag ggagctggtt cacatgatca actgggcgaa gagggtgcca ggctttgtgg
    11761 atttgaccct ccatgatcag gtccaccttc tagaatgtgc ctggctagag atcctgatga
    11821 ttggtctcgt ctggcgctcc atggagcacc cagtgaagct actgtttgct cctaacttgc
    11881 tcttggacag gaaccaggga aaatgtgtag agggcatggt ggagatcttc gacatgctgc
    11941 tggctacatc atctcggttc cgcatgatga atctgcaggg agaggagttt gtgtgcctca
    12001 aatctattat tttgcttaat tctggagtgt acacatttct gtccagcacc ctgaagtctc
    12061 tggaagagaa ggaccatatc caccgagtcc tggacaagat cacagacact ttgatccacc
    12121 tgatggccaa ggcaggcctg accctgcagc agcagcacca gcggctggcc cagctcctcc
    12181 tcatcctctc ccacatcagg cacatgagta acaaaggcat ggagcatctg tacagcatga
    12241 agtgcaagaa cgtggtgccc ctctatgacc tgctgctgga ggcggcggac gcccaccgcc
    12301 tacatgcgcc cactagccgt ggaggggcat ccgtggagga gacggaccaa agccacttgg
    12361 ccactgcggg ctctacttca tcgcattcct tgcaaaagta ttacatcacg ggggaggcag
    12421 agggtttccc tgccacagct tgatgaagat ctgagctctt aattaatgat cataatcagc
    12481 catatcacat ctgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
    12541 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
    12601 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
    12661 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatccg tttaaactaa
    12721 ctttaaataa ttggcattat ttaaagttac atatggcggc cgccctaagg ctcgacctcg
    12781 aaattctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca
    12841 gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc
    12901 accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct
    12961 cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg
    13021 gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg
    13081 gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg
    13141 ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc
    13201 cgaaggtctt ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg
    13261 ttctcctctt cctcatctcc gggcctttcg acctgcatcc atctagatct cgagcagctg
    13321 aagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
    13381 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
    13441 gatccagatc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
    13501 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
    13561 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
    13621 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
    13681 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag
    13741 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
    13801 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
    13861 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
    13921 ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc acgaccccat
    13981 gcatcgatga tatcagatcc ccgggatgca gaaattgatg atctattaaa caataaagat
    14041 gtccactaaa atggaagttt ttcctgtcat actttgttaa gaagggtgag aacagagtac
    14101 ctacattttg aatggaagga ttggagctac gggggtgggg gtggggtggg attagataaa
    14161 tgcntgctct ttactgaagg ctctttacta ttgctttatg ataatgtttc atagttggat
    14221 atcataattt aaacaagcaa aaccaaatta agggccagct cattcctccc actcatgatc
    14281 tatagatcta tagatctctc gtgggatcat tgtttttctc ttgattccca ctttgtggtt
    14341 ctaagtactg tggtttccaa atgtgtcagt ttcatagcct gaagaacgag atcagcagcc
    14401 tctgttccac atacacttca ttctcagtat tgttttgcca agttctaatt ccatcagaag
    14461 ctggtcgagt aactttaaat aattggcatt atttaaagtt aggcgcgcca taacttcgta
    14521 tagcatacat tatacgaagt tatgtcgaga tatttaaata ttagacccag ctttcttgta
    14581 caaagtggtt gatatctcta tagtcgcagt aggcggaact agccttgttt ttttgagcta
    14641 tcacattgtc tttgggctcc ttatttgtct catgactcct accctgatta acttttaaac
    14701 tattaatttg tctcttgact tttcaaagac atgagagaag aaaaaaacac aaaatgtttt
    14761 gttcagaatt ttcagtctaa tgctactgag gaaatgttgt ttttctcctc tctagatgta
    14821 ttagtaattc cagccagtaa tgccagccag gcatggtgtt gttttgtttt ctacaaccac
    14881 ttaggttatt ttggcctctg acagattttc aagatgctta tgactcaaat accccttagg
    14941 ctttgggtat gcattaagcc ttttcttttt tttttcccta gaaaaggtgt aagaatttgg
    15001 agttgctgca gttcaatatg aaggacaact tcagttttgc tgccaccagc agaaacatta
    15061 ccagcagccg tccttttgat aatctcaacg caactggcac caatgagtcc gcctttaatt
    15121 gctcacacaa accatcagat aagcatttgg aagcaattcc tgttctctac tacatgattt
    15181 ttgtgattgg gtttgctgtt aatattgttg tggtctcact gttttgttgt caaaagggcc
    15241 ctaaaaaggt gtccagcatt tacatcttca atctggcctt ggctgactta ctccttttgg
    15301 ctaccctccc tctctgggca acctattact cttatagata tgattggctt tttggacctg
    15361 tgatgtgcaa agtgtttggt tcttttctga ctctgaacat gtttgcaagc atttttttta
    15421 ttacctgcat gagtgtcgat aggtaccaat cggtcatcta cccttttctg tctcaaagaa
    15481 ggaatccctg gcaagcatct tatgtagttc cccttgtttg gtgtatggct tgtctatcct
    15541 cattgccaac attttatttc cgggatgtca gaaccattga atacttaggt gtgaatgctt
    15601 gtattatggc tttcccaccc gagaaatatg ctcagtggtc tgctgggatt gccttaatga
    15661 aaaatattct tggctttatt attcctttaa tattcatagc aacgtgttac tttggaatca
    15721 gaaaacatct gctgaagact aatagctatg ggaagaacag aattacccgt gaccaagtcc
    15781 tgaagatggc agctgctgtt gtgttggcat tcatcatttg ctggcttccc ttccatgttc
    15841 tgaccttctt ggatgctctg acctggatgg gtatcattaa tagctgtgaa gttatagcag
    15901 tcattgacct ggcacttcct tttgccatcc tcctgggatt caccaacagc tgtgttaatc
    15961 ccttcctgta ttgttttgtt ggaaaccgct tccaacagaa gctccgcagt gtgtttagag
    16021 ttcccattac ttggctccaa ggcaagagag agactatgtc ttgcagaaaa ggcagttctc
    16081 ttagagaaat ggacaccttt gtgtcttaaa tctgttagtg ggatgcatgt aatcagccta
    16141 gccattggtt tggaggccca cacaaatgat ctttaagtgg catcagtata atacagttct
    16201 ttgctttatc taatctttac ttactccccc gagaacagga agtcaagtag aactgtaaat
    16261 ctttatactc caccagcttt cagtgatagt gccttctttt gctggtcctt tggcatgaga
    16321 ttgtcatatg tgagctagat ctataatcta gaagtatctg ggggaattat cccaacttat
    16381 aattaacaac aaattatgag tggtgatttg acatctcaga cttctccctg gaaaatgctg
    16441 gcatttctta gtggagtttt ttgtccattt tcatcagatt tcttttttct tgaacaaagg
    16501 ccaatttaaa cttcttatac tatccaacca tatgatatag catgagaggt gagcactaag
    16561 tttagcatga tatactcttc tatatatgcc ataggttggt agtggcttat tcagtctcta
    16621 ggtatagagt ttctcctttt aaagaaattg taagttgtgt tccttttcca tttcactcaa
    16681 gtatagcttt tgtacttatt ctacagctac acactgagca gatctagaat gtagattaaa
    16741 tcacacatct gtcttagctt attcttgcag ttatagaaag tacactattt agtaaaacag
    16801 aactgcaatg aaaagtattt tagtatccac aaaactgaat atacactttg aaaatttttc
    16861 atccattttg actcttgttt attctattct cttctgatga tttttgaata caacaacaaa
    16921 acactgtatt atgacactac gtaaaggtca ctttttaaat ttttaacctt ttgaacatgg
    16981 tgctttgata tattcaatga tgacttgagt ttaattattc atgcttttgt tctgggcttc
    17041 gtcccaaaat atctctttga ccctgaaaaa gagagcattc tttaattctt taactttgta
    17101 ataaagtgca aactggcatg ggaaaaggtt atgtcagact ggaagtttga tgccttcttg
    17161 ggggtaaaca gacccagcaa atggcaagtt tggtgtccaa caaggaactt gtcagaacaa
    17221 agactccctg gggagtagtt tgaatctgca tttctgggca cagttccaga atgtataaga
    17281 gtctgtgaag gtgatttaaa gcaagcccag gtccacagaa ctcattctta acacgagtac
    17341 atctcttaca ttagaggaat ataatacctg aagctgtgtt acctaaagtt tactcaaact
    17401 tctcaataaa tattaattca gaagttaaag atgtcattct ctgcctgtcc catattatac
    17461 caggtcacct aagaccttcc tggattgatg ctgacctatg aggtagattc aaagttctgg
    17521 gaacttaaca tttctgtcag attccaggcg ttttaggttg aagaatcctc tcatacccct
    17581 tccttggaaa accctgattt catgtattca tgttaatttt tagtaaaaac aaatagctaa
    17641 atatgtaatc agttatgact ttgtgtttta agcaatttta cacaaaatct cgtaaaaata
    17701 aaatcattac tgggagacca tgtgtgcttc ttatttttta actgtctcca ggtttagact
    17761 gctgccttct cttagtcagt ctcctccagc aaatccccat tcataattct cagtttcagc
    17821 ctacatgatt actcattcag gtcaataaga aacaggcata tgaaaagcat atgtcggaaa
    17881 tgaattctga tacctcctgg gggttcaaat gacaaactcg gtgtcaacca agcaagctgt
    17941 caggcacagc agtgaatcgt gggctttcga atgtagacta tttcttgttc atgttatttt
    18001 ctctaccaca atcataacta ttaaatcctt cccaccccct gggacttctc ttcatcctgt
    18061 ttgagatttt taaagactgc tttcaggggc tggagagatg cattgctcca tagttaagag
    18121 cactggctct tccagagatc caggttcaat gatcagcaca cacatggaac ctaacaaggg
    18181 tctgtaactc aagttgcagg ggactaacac cctcttccag tctccatggg ctccagtttt
    18241 tcacatggag atggcgcaac gcaattaatg ataacttcgt atagcataca ttatacgaag
    18301 ttatggtctg agctcgccat cagttcaata ataatagtaa agactggctt ctgcttccat
    18361 aggaggccag tggtgaacca tgagggccat tgttttctag ttattccgag aattgtgacc
    18421 tgaacagata atgcaggacc atgtgagttc tgactgaggg tatatttatg gctacaaata
    18481 ccctctttcc ttcttgaaaa tgtaaatgcc aggtcactta ttaagatgat atgaaaaatt
    18541 ttattctttc catttggcca tttatcaaga tttcccctat gaagggagca atgtgtgact
    18601 ggtgctgtga caggtgcgtt atcccatctc attctcccaa ggactaaggt attttccatc
    18661 atgacacaag taagaaaggg aggttcacac caaccaggca gcatacaaga cctggctcaa
    18721 ggtccccctg acacatatag agcagaggac tgtctggtct ggcctcagtg agagaagaca
    18781 cacctaaccc tggagagact tgagctcccg gggagtgggg aggcctgggg cgggaggggg
    18841 agaaacaccc tcttccagac agggagagga gggatgggat gaggaactgt ggggggggag
    18901 gaccaggaga ggggcaatgg ccggactgtg gactgtaaaa aatataaaag tcataaataa
    18961 taataataat aataataata aggcgaagct cagatcagtc ttaactgatc tctaaaacag
    19021 atcaaatggg aaagaccaga gaagtattta gtgccttaaa tatcagtcct aaattaccaa
    19081 acaagtattt gaaagacact cgtgaagtct ttcaaccttt attccaaaca taactgagtc
    19141 aagagtttct aggcaggtgg tgtgcttgtg cttagaagga aattgaggag gtcgcttgat
    19201 aaggctttct cccttgttct ccagtgcttc catcttttgt actaagtttc acagagccac
    19261 atggaataac cagtgtgttg tacataaagc aagacaggag tctcaaacac ttattttgag
    19321 gaataaatat gttgatctga agtttttctc tgacaataga tttcctgggg ttatcaaaga
    19381 tgtttctctc ttgcagcttg ctgacaatac attttttatg agaaagaaat attaattcca
    19441 catcatgttg gcttaaatca aatcaattaa atagcatcat ttaaaaaaaa tctaatctgt
    19501 ttgaatcctg cttccaaaga agaaaatgtg aagtgtggag ataagcctat tctagtctga
    19561 ctctgtcaca gggatcggaa cctgggtacc taactctctg gtgacctcac ttctcaacaa
    19621 taggaagcca caaacttgac aagatgaagg agcacacagg gggaatggag taagaaaaga
    19681 taggaaggaa atctaaaata tatttacttg ttttcgagat ttccagataa actgtattag
    19741 cactgatgat ttatgcaact taacagaaat gcattagcca tcactgaaac ctcatcagta
    19801 ccttggggct gaatttccaa gcgtgcttgc ataaattcac ctattaaagc atctttggga
    19861 agagatatat ttttcttttt aaaatttctc cactctagga tgagagaatt ctttctaaga
    19921 tgagaactca ttggtatgtt ttccttctta tacagtcagt acttattgaa atttttaact
    19981 atttaaaatg agccacatat ctaatcttca ctaacaacac gataaatatt gctatagata
    20041 agatactaca tatatgtaca tttttctttt aatctattat atatcaagta tgtcattcat
    20101 caacctgtgt aatagtgatt taatatttta aatattttga ccctgcactt ccatctgttt
    20161 tactggacaa ttttcttaca ccatttagct cttctcttct ataaggtcct aaagcatcag
    20221 ggctttaatc aatccatgat atcatgcctg attaccattg ctcaatgatg gaaagtgctg
    20281 ggaaatacaa ccattttatt ggttaggtct atacagttgt tgatgctaca tctttagcaa
    20341 tcaatccaca ggaaattaga tggctctgta gtttggcgta gattgctaca cctgcccact
    20401 taagtagctt ggaagtgatg tatgcataag acatctgaaa agaatgtaag aaaaagaaag
    20461 atgtttcttt ttctttctct tgtaacataa acactttaca actgatgtat gccacagttg
    20521 atgttggcta aaaacattta tttctttaaa gcacatccac ggagccaata agaaagagcc
    20581 gaaatccatc agagagatat accataccag ataccagttt tcaaacactt tcttcaagaa
    20641 acctcttcta agcaaccaga attaatttta aatttatcct caaaatacag aagactgcca
    20701 gattaagggt aacgtggcta atgttgccct ataaaaaatg tgtattggtg tttttgcctt
    20761 catgatatct gtacaccatt tgcatgactg gtgcccagag aagacagaat ttgttgttgg
    20821 atcccctggg actggagtta cagacagtta tgagccacca tgtaggtgct agagattgaa
    20881 acccagttcc ctgggagaat agccagtgtt ctttcctact gagccatgtc tatagcccct
    20941 aatgtttttt cttgatgaca gaagtgggag ccatctatcc aatatataca aagagctcaa
    21001 gaagctggac tgcagaaaat caaataaccc tattaaaaat ggggtacaga gctaaacaaa
    21061 gaattctcaa ctgaagaata ccgaatggct gagaagcacc tgaaaaaatg ttcaacatct
    21121 ttaatcatca gggaaattca aatcaaaaca accttgagtt tccacctcac accagtcaga
    21181 atggctaaga tcaaaaattc aggtgacagc agatgctggc gaggatgtgg agaaagagaa
    21241 acactcctcc attgttggtg ggattgcaag ctggtatgac cactgtggaa atcagaaaat
    21301 tagacatagt actaccggag tatctagcaa tacctctcct gggcatatat ccagaaaatg
    21361 ttccaactgg taaaaggaca catgctccac tatgttcata gcagccttat ttataatagc
    21421 cagaagctgg aaagaaccca gatgtcgctc agcagaggaa tggatacaga aaatgtggta
    21481 catttacaca atggagtact actcagctat taaaaattga atttaataaa attcttaagt
    21541 aaatggatgg atttggagga tagcatcttg agtgagataa cctaatcaca aaagaagtca
    21601 ttagatatgc actcactgat aagtggatat tagcccacaa acttagaatg cccaagatac
    21661 aagttgcaaa acacacgaaa ctcaagaaga aggaggacca aagtgtggac acttcatttc
    21721 ttcttagaat ggggaacaaa atacacatgg aaggagttac agagacaaag ttctgagcag
    21781 agcctgaagg aacgaccatc cagaaactgc cttactttgg gatccacccc ataaacaacc
    21841 accagtcact aggcagatgc caacaagagc ctgctgatag gagtctgata tagctgtctc
    21901 ctgagaggct ctgccagtgc ctggcaaata cagaagtgca tgctcacagt catccattgg
    21961 acggagtaca aggttcccag tgaaggagcc agagaaatac ccagagagct gaaggggcct
    22021 gaagccccat aggaggaaca tcaatatgaa ctaaccagta ccccccgagc tccttggaac
    22081 tatactacca atcaaagaaa acacatggtg gaacttatgg ctctagctat atatgtagta
    22141 gaggatggct tagtaggttc ttaagaataa gaacttggag tttcttaggg gaaggtgtag
    22201 gtgttgattc ccttcctttt gggaatctca cactctcctg aacctaagat caacatttta
    22261 atgtgtagag gaaatgctgt gcgcctgcca ttttcctgaa aagattgaaa ccattttaag
    22321 cctcttctga agccaagatt ctgtcctagt ccttccctta aaaacaacga atctgtgttt
    22381 atcaaaagac tccactggag tgataattcc cattataaaa acctttacta tgacacattt
    22441 ttgttgctgt aacagcttga aatgtttgta aataacaact ttgaaatggt atttcccccc
    22501 ttctctgaaa aagaaaaaaa atgcctccct aggggttatg ctattatcta ggtccctaac
    22561 ccaaattcat gttgaatcgc tggtgctcaa tgcaacttta tttagatatc tggaagtagc
    22621 taaggataaa ataaaattgg ggttccaata gtctgtttgg tagcattata agagaaa
//