LOCUS       43267#pL1L2_GT2_LF2A_nEGFPO_T2A_CreERT_puro#L3L4_pD223_DTA_T_spec        21182 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT2_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 43267
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000032416
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            8394..8471
                     /db_xref="ENSEMBL:ENSMUSE00001207423"
                     /rank="2"
                     /note="ENSMUSE00001207423"
     exon            7150..7181
                     /db_xref="ENSEMBL:ENSMUSE00000427405"
                     /rank="1"
                     /note="ENSMUSE00000427405"
     misc_feature    4275..9269
                     /note="5 arm"
     misc_feature    9297..9321
                     /note="B1 Gateway"
     misc_feature    9334..9381
                     /note="Frt"
     misc_feature    9382..9881
                     /note="mouse En2 intron"
     primer          complement(9450..9471)
                     /note="LRPCR LAR3_EN2"
     primer          complement(9346..9369)
                     /note="FRT3"
     primer          13540..13558
                     /note="seq primer NF"
     misc_feature    13600..13607
                     /note="RE sites"
     misc_feature    15400..15433
                     /note="loxP"
     misc_feature    complement(13608..13639)
                     /note="Rox"
     misc_feature    complement(15360..15391)
                     /note="Rox"
     misc_feature    13640..13661
                     /note="polylinker"
     CDS             14220..14816
                     /note="puromycin N-acetyl transferase"
     misc_feature    14335..14340
                     /note="Mutated RsrII site"
     intron          9882..10256
                     /note="mouse En2 intron"
     misc_feature    10254..10256
                     /note="Splice Acceptor"
     exon            10257..10303
                     /note="mouse En2 exon"
     primer          10157..10178
                     /note="seq primer FCHK2"
     misc_feature    10304..10426
                     /note="1Dleader"
     misc_feature    10427..10478
                     /note="F2A"
     misc_feature    10574..11290
                     /note="EGFP"
     misc_feature    10484..10573
                     /note="3X NLS"
     misc_feature    13356..13594
                     /note="SV40 polyadenylation site"
     misc_feature    12389..13333
                     /note="ERT2"
     promoter        13672..14183
                     /note="PGK promoter"
     misc_feature    14927..15359
                     /note="PGK pA"
     primer          13414..13437
                     /note="LRPCR RAF5"
     misc_feature    11300..11353
                     /note="T2A"
     misc_feature    11350..11350
                     /note="A to G to kill RsRII site"
     misc_feature    11354..12379
                     /note="Cre"
     misc_feature    10272..10304
                     /note="FCHK lf2a_lacz_gt2"
     misc_feature    10272..10287
                     /note="FCHK QC prefix region"
     misc_feature    10288..10298
                     /note="FCHK QC critical region"
     misc_feature    10299..10304
                     /note="FCHK QC suffix region"
     misc_feature    15456..15476
                     /note="B2 Gateway"
     misc_feature    9270..15506
                     /note="Synthetic Cassette"
     exon            15869..16022
                     /db_xref="ENSEMBL:ENSMUSE00000197007"
                     /type="targeted"
                     /rank="3"
                     /note="target exon 1 ENSMUSE00000197007"
     misc_feature    15507..16172
                     /note="Critical Region"
     misc_feature    16196..16229
                     /note="Downstream LoxP"
     misc_feature    16173..16252
                     /note="synthetic loxP region"
     exon            20722..20912
                     /db_xref="ENSEMBL:ENSMUSE00000225561"
                     /rank="5"
                     /note="ENSMUSE00000225561"
     exon            20480..20638
                     /db_xref="ENSEMBL:ENSMUSE00000225568"
                     /rank="4"
                     /note="ENSMUSE00000225568"
     misc_feature    16253..21182
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggaagggaa taacaggcct acatggcttt ctttggactc tgatggtatc
     4321 atttccccga ccccaccccc caggacagtc aatgtctgct cacctgttgg agtacagctc
     4381 ctctcacctc tctgggtcaa taagccattc cgttctccca acatttgttg tcctaacatg
     4441 ttggatttgt tgttgtttgt ttgtggggtt taatttgtat cttttatact aatttattta
     4501 atatatgtga cacatacaca cggggcttgt gggaaggtca gaaggtgact cgcaggagta
     4561 agttctttcc attatgtagg tcccaatgat tgaattcatc gaatttgtaa gtacccacag
     4621 agccattttg atggccctcg tgctgttttg aacatcaagg tctgtggtgg tttgaatatg
     4681 tttggttcag ggagtggcac tattaggtat ggccttattg acagaagtgt gtcactgtgg
     4741 gtgtagcctt taagatactc atcctagctg cgtggaagcc agtcttcttc tataagcctt
     4801 cagatggaga tgtagaactc tcaactcctc ctgtaccatg tctgcctgga tgctgccatg
     4861 tttttgcttt gttgataatg aactgaacct ctgaatctgt aagccagtcc caattaaatg
     4921 ttgtccttat aagagttgcc taggtcttgg tgtctgttca cagtctggtg tatagtgtga
     4981 aactgggtcc tctatagagg tgttcagtgc tcttgaccac tgagacagct ctccaacccc
     5041 tgtaataata tgaacatttt tgtagaggtt ttgaggtaga catatttgtg tgtatgtgtt
     5101 gactcaaaaa aaaataaaac cataactgca actcattctg tagcccagga gggaccttaa
     5161 acacaaaaca gtcagcttgc ctctgcctcc caagtgctgg gatcccaggc ctgagccacc
     5221 acgccaacta cattgttttc attttttgtt ttcttcccag aacttgctta ttaggaaaca
     5281 aagaaagaaa caacatgaaa caacgaataa ataaacaaaa cttttttttt ttctttgagg
     5341 taagaccttt cactgtgtgg cccaggctgg tcttagactg gtgatgtttg tgcgtcagcc
     5401 tctggaggct ccgggtgaga gagaggggtg tacatcacca ctcctggctc ccattgctca
     5461 ttcgtgcatt tgcttctgtt tccagcccag ctcctccagg tgtgggctaa ggccgtcttg
     5521 ctttctgatg tattgtcagc ccctagcgtg gcacacactg aaagtactcg atgaaggaat
     5581 gtcgcctctt ccagggattg gcttagctct aaataatgca tctcgtgaga gccagtgagt
     5641 ccccaccgga caggtgacta caggacagaa aggctgtaca gctctagctt cgtgttgctg
     5701 ccttagaatc agcttcctga tcagctgtgc ctacggatgt gtaggcattt ataaagtact
     5761 cgtcttaacc agtttggtgg cacacacctt tagtcccggc tctctggtgt atagtgtttt
     5821 acaccatatg catgcagtgt ccacggaggc ctgacgaggt gacagatccc tggaactaga
     5881 gttacaaaca attgtgagtc gccatatggg tgttggtaat agaaactggg tcctctatgg
     5941 aggtatccag tgcttttgac cactgagaca gatctccaac ccctggaata atgtttttgt
     6001 agagattttg atgtagacat gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgttg
     6061 actcaatggg tcatcttgct ggcctaaatt ttatttttct tagtagtagt atctgtaaca
     6121 aatgattata gattttatac tctttttttt ttttttgaga ctttatagct caggcttgtc
     6181 ttgaattaga gattatcttt ctgtctcttg agtcatatat ttcattatac ccaacttttg
     6241 tgatttccca cccccttttt catgccaggt tgccactctg tagcccaggc tagcttcatc
     6301 tatagcagtc ttcctgtctt ggcttcccaa gtgctggggt tgtaggcatt acaggcatga
     6361 gctacctctc ttaacaagat atttttattt tgatattaca catttcacct tgtcataatt
     6421 gctgatttta tttattacat atagcatatt agcaacaaca atcactttta tccactgagc
     6481 acctaagtta caaatgtttt tctattttta tttgtattag tttttgtttt gttttgtttt
     6541 tttttcaaga cagggtttct ctgtgtagcc ctggctgttc tggaactcac tctgtagatc
     6601 gggctggctt caaactcaga aatctgcctg cttctgcctc ccaagtgctg ggattaaagg
     6661 catgcaccac cactgcccag cacaaatgtt tattatgtgt gtttttgggt tgtgagacag
     6721 ggccaactct atagcatgag agttttcaac tcatggccct tctcacgcca cagcctacca
     6781 agtatcatga tgacagctct gagccaccat acctgacttc aacatgaacg ttactcctga
     6841 cacagagggg agaacaaaag gcagctcgtg tgagcacaga cagcctgtgt gggggcggaa
     6901 ctcagaccat aagccacatc cattgcttct gtgccctctt gcctagtgcc atgggcagta
     6961 cctcttctaa agctttcgaa acatgccttc ttccccgttt cagggccttc tggaagccag
     7021 tctgacttaa aatccctagg ggaaagtttc gaactcattc cccatgctct tttccccagc
     7081 tggggctggg ggctagggga gtggcttcac aggagacaga aataaaatgg agggaagcct
     7141 gggctgtgcc tggccacgcg gtattgccag gaggctggga ggtgaggggg aggtgaggag
     7201 ctggggtata tggctactag agagcagatg gtgggggatt gccgtggtgg gtatataagg
     7261 gacatggttg gaacaagtat gcttgggtag tgttgggttg tggtgagggg actctatagg
     7321 ggtgacccaa ggagaacctg cgttaatcag aatcgaagag tactttgact gctttgtcat
     7381 ggctgaggtc gcagttctgt cccctgccca ctcctgggtt cttctctgct ccttgtgcta
     7441 agggctgaaa acagaggcat caaggacaca ggagcttcag agacatggac agggggtggg
     7501 caggatggag ggtccttctg ccagttattg gggaaggttt tcctgaggcg gcaacgggga
     7561 acctgggact tctaggtggc ctgtgtggag tcctgagtat gacacaagct gtaccacagg
     7621 taaagagact ggctgtgcag ggccttacag ccccagcagg caagaaccag agaacagcac
     7681 tacagggagg aaaacaggca tgtgagccca ataccacccc aaccagagct gggatgccca
     7741 gactgatacc ttgagttaca aaccatagta gttacctaaa ttagttaagg ctccatagaa
     7801 gtgcagagtc agctcctttg tcacatcagt catgttgcca agggctcagt gtgggtagca
     7861 gctatggtac tggagagcac aaacctagat ggtcttcagt agaaacgttc tcttggccag
     7921 cactaaacct caggagggct ctattgctca agggcccatg gggcaaagca ggctccaccg
     7981 tctggctgcc cgtggagttt ctggactata ggtgactttc aggtccctca cagcaccagg
     8041 aggtgtgact cacctcctgc cagcgtgaag cggactctgt cccctgagac agggacaaat
     8101 cagtgcgggc ctctttagca ctgacttcag cttctccact cagtgtgacc acagcttggg
     8161 tcggagaaac aatggagtga gtgaggctga ggctggggag cagctgggga gtgggtgggg
     8221 gtggtggacg ctggctcagt cgtagttact gtggactccg gggaaatggg tggtcagatg
     8281 agagactggg gcagagggtt gctgcgtggc ggttggggcg cattgggtca aagaaagaaa
     8341 gagaaggacc cctcccttct cgctaaccag ataagcttct gcctgtgttc taggtccaac
     8401 acaatccaac tgacaaggat ggactacgcc atgaagtctc tcagcctcct gtaccccagg
     8461 tcgctgtcca ggtgagggga gccaggaaca gaaaggaagg gggaaaccaa ggagccatca
     8521 gttagaactt cttaaacttt taatgggtat aaaagccatt taggggctgg ctgtggctgt
     8581 gtgtgtgggg gggggcggtt atgcttttaa ccccagcact caggaggcag aggcaggaag
     8641 atctttgtga gttcaaggtc agcttgatct ccagttccag gttagccagg gctacagagg
     8701 ctaccctgtc tcagagaaac aaagtcatgt gggtgtgtag tggaaacaca gatacgggca
     8761 ggcatcgtgg ctggcatata atttgaatcc tggcacttgg ggagcaaagg caagtggatc
     8821 ttggtgaatc tgaggacagt gtagtatgca tagaaagttc caggtcaact agagctacat
     8881 agtgagatac tatctcaaaa acagacaagc aagcagagaa tccattaaaa caaaacaagc
     8941 aaaaaaaaaa aaaatcttac cataagccgt gacagatctg atttagatct gagctgccta
     9001 cagtggtgtc caagcctgca gtctggacaa gtgccaggtt acactcttct cgagtgccac
     9061 cattgtggtt cctgtcatat ctggagtaga gagggccagg ctctgcactg ggcaccttca
     9121 ttgctgatct cccaggagcc tcatgcctac actggggagt gggggctaat atgtcaagtc
     9181 aatagatggg gaaactggca agctgagcat tctgtcacct gtcgcctagc aactctgtag
     9241 caaagacaaa ctttgaactt gaacccagaa aggcgcataa cgataccacg atatcaacaa
     9301 gtttgtacaa aaaagcaggc tggcgccgga accgaagttc ctattccgaa gttcctattc
     9361 tctagaaagt ataggaactt cgaacccttt cccacaccac cctccacact tgccccaaac
     9421 actgccaact atgtaggagg aaggggttgg gactaacaga agaacccgtt gtggggaagc
     9481 tgttgggagg gtcactttat gttcttgccc aaggtcagtt gggtggcctg cttctgatga
     9541 ggtggtccca aggtctgggg tagaaggtga gagggacagg ccaccaaggt cagccccccc
     9601 cccctatccc ataggagcca ggtccctctc ctggacagga agactgaagg ggagatgcca
     9661 gagactcagt gaagcctggg gtaccctatt ggagtccttc aaggaaacaa acttggcctc
     9721 accaggcctc agccttggct cctcctggga actctactgc ccttgggatc cccttgtagt
     9781 tgtgggttac ataggaaggg ggacgggatt ccccttgact ggctagccta ctcttttctt
     9841 cagtcttctc catctcctct cacctgtctc tcgacccttt ccctaggata gacttggaaa
     9901 aagataaggg gagaaaacaa atgcaaacga ggccagaaag attttggctg ggcattcctt
     9961 ccgctagctt ttattgggat cccctagttt gtgataggcc ttttagctac atctgccaat
    10021 ccatctcatt ttcacacaca cacacaccac tttccttctg gtcagtgggc acatgtccag
    10081 cctcaagttt atatcaccac ccccaatgcc caacacttgt atggccttgg gcgggtcatc
    10141 ccccccccca cccccagtat ctgcaacctc aagctagctt gggtgcgttg gttgtggata
    10201 agtagctaga ctccagcaac cagtaacctc tgccctttct cctccatgac aaccaggctc
    10261 ccaggtcccg aaaaccaaag aagaagaacg cagatcgcag atctctagag tcaccgagtt
    10321 gctttaccgg atgaagaggg ccgaaacata ctgtccaagg cccttgctgg caatccaccc
    10381 aactgaagcc agacacaaac agaaaattgt ggcaccggtg aaacagactt tgaattttga
    10441 ccttctcaag ttggcgggag acgtcgagtc caaccccgga ccggtgcatg tggaccccaa
    10501 gaaaaagcgg aaggtggacc ctaaaaagaa acgcaaagtg gatcctaaga aaaaaagaaa
    10561 ggtgcacgtg tccatggtgt ccaagggcga ggaactgttc accggcgtgg tgcccatcct
    10621 ggtggaactg gatggcgacg tgaacggcca caagttcagc gtgtccggcg agggcgaagg
    10681 cgacgccaca tacggaaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
    10741 gccttggcct accctcgtga ccacactgac ctacggcgtg cagtgcttca gcagataccc
    10801 cgaccatatg aagcagcacg acttcttcaa gagcgccatg cccgagggct acgtgcagga
    10861 aagaaccatc ttctttaagg acgacggcaa ctacaagacc agggccgaag tgaagttcga
    10921 gggcgacacc ctcgtgaaca gaatcgagct gaagggcatc gacttcaaag aggacggcaa
    10981 catcctgggc cacaagctgg agtacaacta caacagccac aacgtgtaca tcatggccga
    11041 caagcagaaa aacggcatca aagtgaactt caagatccgg cacaacatcg aggacggctc
    11101 cgtgcagctg gccgaccact accagcagaa cacccctatc ggcgacggcc ctgtgctgct
    11161 gcctgacaac cactacctga gcacccagag cgccctgagc aaggacccca acgagaagag
    11221 ggaccacatg gtgctgctgg aattcgtgac cgccgctggc atcaccctgg gcatggacga
    11281 gctgtacaag cctgcaggcg agggcagagg aagtcttcta acatgcggtg acgtggagga
    11341 gaatcccggg ccgtccaatt tactgaccgt acaccaaaat ttgcctgcat taccggtcga
    11401 tgcaacgagt gatgaggttc gcaagaacct gatggacatg ttcagggatc gccaggcgtt
    11461 ttctgagcat acctggaaaa tgcttctgtc cgtttgccgg tcgtgggcgg catggtgcaa
    11521 gttgaataac cggaaatggt ttcccgcaga acctgaagat gttcgcgatt atcttctata
    11581 tcttcaggcg cgcggtctgg cagtaaaaac tatccagcaa catttgggcc agctaaacat
    11641 gcttcatcgt cggtctgggc tgccacgacc aagtgacagc aatgctgttt cactggttat
    11701 gcggcggatc cgaaaagaaa acgttgatgc cggtgaacgt gcaaaacagg ctctagcgtt
    11761 cgaacgcact gatttcgacc aggttcgttc actcatggaa aatagcgacc gctgccagga
    11821 tataaggaat ctggcatttc tggggattgc ttataacacc ctgttacgta tagccgaaat
    11881 tgccaggatc agggttaaag atatctcacg tactgacggt gggagaatgt taatccatat
    11941 tggcagaacg aaaacgctgg ttagcaccgc aggtgtagag aaggcactta gcctgggggt
    12001 aactaaactg gtcgagcgat ggatttccgt ctctggtgta gctgatgatc cgaataacta
    12061 cctgttttgc cgggtcagaa aaaatggtgt tgccgcgcca tctgccacca gccagctatc
    12121 aactcgcgcc ctggaaggga tttttgaagc aactcatcga ttgatttacg gcgctaagga
    12181 tgactctggt cagagatacc tggcctggtc tggacacagt gcccgtgtcg gagccgcgcg
    12241 agatatggcc cgcgctggag tttcaatacc ggagatcatg caagctggtg gctggaccaa
    12301 tgtaaatatt gtcatgaact atatccgtaa cctggatagt gaaacagggg caatggtgcg
    12361 cctgctggaa gatggcgatc tcgagccatc tgctggagac atgagagctg ccaacctttg
    12421 gccaagcccg ctcatgatca aacgctctaa gaagaacagc ctggccttgt ccctgacggc
    12481 cgaccagatg gtcagtgcct tgttggatgc tgagcccccc atactctatt ccgagtatga
    12541 tcctaccaga cccttcagtg aagcttcgat gatgggctta ctgaccaacc tggcagacag
    12601 ggagctggtt cacatgatca actgggcgaa gagggtgcca ggctttgtgg atttgaccct
    12661 ccatgatcag gtccaccttc tagaatgtgc ctggctagag atcctgatga ttggtctcgt
    12721 ctggcgctcc atggagcacc cagtgaagct actgtttgct cctaacttgc tcttggacag
    12781 gaaccaggga aaatgtgtag agggcatggt ggagatcttc gacatgctgc tggctacatc
    12841 atctcggttc cgcatgatga atctgcaggg agaggagttt gtgtgcctca aatctattat
    12901 tttgcttaat tctggagtgt acacatttct gtccagcacc ctgaagtctc tggaagagaa
    12961 ggaccatatc caccgagtcc tggacaagat cacagacact ttgatccacc tgatggccaa
    13021 ggcaggcctg accctgcagc agcagcacca gcggctggcc cagctcctcc tcatcctctc
    13081 ccacatcagg cacatgagta acaaaggcat ggagcatctg tacagcatga agtgcaagaa
    13141 cgtggtgccc ctctatgacc tgctgctgga ggcggcggac gcccaccgcc tacatgcgcc
    13201 cactagccgt ggaggggcat ccgtggagga gacggaccaa agccacttgg ccactgcggg
    13261 ctctacttca tcgcattcct tgcaaaagta ttacatcacg ggggaggcag agggtttccc
    13321 tgccacagct tgatgaagat ctgagctctt aattaatgat cataatcagc catatcacat
    13381 ctgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
    13441 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
    13501 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
    13561 tgtccaaact catcaatgta tcttatcatg tctggatccg tttaaactaa ctttaaataa
    13621 ttggcattat ttaaagttac atatggcggc cgccctaagg ctcgacctcg aaattctacc
    13681 gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg
    13741 gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc
    13801 gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca
    13861 ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac
    13921 gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt
    13981 ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg
    14041 gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtctt
    14101 ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt
    14161 cctcatctcc gggcctttcg acctgcatcc atctagatct cgagcagctg aagcttacca
    14221 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac
    14281 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccagatc
    14341 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
    14401 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga
    14461 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt
    14521 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
    14581 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca
    14641 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg
    14701 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
    14761 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac
    14821 gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc acgaccccat gcatcgatga
    14881 tatcagatcc ccgggatgca gaaattgatg atctattaaa caataaagat gtccactaaa
    14941 atggaagttt ttcctgtcat actttgttaa gaagggtgag aacagagtac ctacattttg
    15001 aatggaagga ttggagctac gggggtgggg gtggggtggg attagataaa tgcntgctct
    15061 ttactgaagg ctctttacta ttgctttatg ataatgtttc atagttggat atcataattt
    15121 aaacaagcaa aaccaaatta agggccagct cattcctccc actcatgatc tatagatcta
    15181 tagatctctc gtgggatcat tgtttttctc ttgattccca ctttgtggtt ctaagtactg
    15241 tggtttccaa atgtgtcagt ttcatagcct gaagaacgag atcagcagcc tctgttccac
    15301 atacacttca ttctcagtat tgttttgcca agttctaatt ccatcagaag ctggtcgagt
    15361 aactttaaat aattggcatt atttaaagtt aggcgcgcca taacttcgta tagcatacat
    15421 tatacgaagt tatgtcgaga tatttaaata ttagacccag ctttcttgta caaagtggtt
    15481 gatatctcta tagtcgcagt aggcggaaat actctatagg ctgcctcccc cattttaccc
    15541 aactttacag cgaactgaac aaggccacag agcagagtag cccagagggg aatgtggcta
    15601 gtccaagaac cagacaacac tgacagtttg gggacttgtt agacatagaa acccttgggc
    15661 tcaactcaca gccacagaag tgagtctgat ttaagcaaga tttctgctga ttttcctgcc
    15721 catccaaatt ggaagcacac cctcagatgg gctttgagaa agccacctgg aggggccatg
    15781 attggctgga gggaagggtc agatgtgagc tcagtaggca ggggtgggca gcaggcaggt
    15841 catctgatgt tcaaactaat gtccccaggc atgtggcagt gagcacggca gtggtgaccc
    15901 aacagctggt gtctaagccc agccgggaga ccccgagggc caggccctgt cgtgttagca
    15961 ccgcagatcg gaaggttcgc aaaggcatca tggctcacag cttggaggac ctcctgaaca
    16021 aggtaaggaa tttgcctgta ctctgcatcg gctctcctca ggcctgagag ttgacactgc
    16081 taaccacttc agtctttcag gcttgtgtgt gcgtgcatgc gcatgtgcgt gtgttgtttt
    16141 cttaagaaag cttcctttca ctgggcggtg gtgagatggc gcaacgcaat taatgataac
    16201 ttcgtatagc atacattata cgaagttatg gtctgagctc gccatcagtt caaaaaaggt
    16261 tcctttctga tgtcagcgct ggagggttgg cattacgtgc ctggagagct gtgtgtgtga
    16321 cagagaggtg acatggtcaa gtgacagagg gagggcagta ctttgctgtc ttacacgcta
    16381 atagtggctg ggtggtattt tcttcatgaa aaggctctca gttctttttc ctattacact
    16441 tggaaaatga gccaggtatg gtgtgtcatg cctgagagcc caggacttga cacacacaca
    16501 cacacacaca cacaccccaa catccctttc actcacacac agtaaaacta cacaaaacat
    16561 aaaaacaacc ttccagaagt gtcaggagtt gaggaaacag gatttcagtg cttccggtct
    16621 gcatctccta cccgccccca cactactttc cctgcatctg tctctcccaa gctggaactt
    16681 ccaggaaagc ctttctttga aaaattagta ctccctgatt agtggaaacc cagggctgcc
    16741 agctgcggta ctaggagaag catgcagtct ggagagcagg gccctgttcc catcatgcct
    16801 ctccctgccc ctaccccctt ctaattttgc ttctacctgt tgtgtcgacc cattagtatt
    16861 agctgcatgg ggctggaacc cttacatctg caggtccagg tgccccacac aagccaaacg
    16921 tttgaagtag gttttcagtt acaatcaatt tagcatgcag agagcgtaga ggtttctaac
    16981 acattcatgc aaatttactc tgctcttcct aggaactgat tttttttttt tttttttttt
    17041 tttgtctatg agacatgtag ctgaacctgc cttgaatcaa aaggactggc ttactcttgg
    17101 ctccagggac cacttccatc attagggtct tcttatcata tccccttcca acactttaca
    17161 tccatacccc ccaccccaga cattgggtca tggctaaggc tgggggtttt ccttgtcagt
    17221 gggtacttta agaaaggctt agagttgtag aatttgagac ctcctagtta gggttccatt
    17281 gccagggaac acagggagcc ctgggggact ctgtaacata gacaggtcat aagggactct
    17341 tactgccggg gcttggagga tgtcagcccc ctggactttg attccgtgtt tggcagcaca
    17401 aaggtgtgga cagagtggag gggagagcat tggagatgtg gttaccatgt agccatgtca
    17461 ttccaaagat ctcacaagtc tgctcctggg aggagcacag ttagttagca tggatgagct
    17521 aggagcctca cctggactgt taagtcagca atgctctctg cgtgctactg gaggaattta
    17581 aattttactt tttaatgatt agccacttgt tttgtgtgtg tgtgtgtgtg tgtgtgtgtg
    17641 tgtgtgtgtg tgtgtgtgtg tgcacgcgtg catgtcacgg gacacagata gacatcggag
    17701 tatggaagtt ggtttttctc tttccatcat gtgggtctga gattgaatcc aggccagcaa
    17761 gcttggctct aagcaccttt acctgctgag ccatctcact ggcacttaca ttccttttgc
    17821 ttatttaagt tagtttcttg gtttgcctgt aggcacttag gcagggcttc agtctttagc
    17881 tcagacttcc ttggaactca ctatgtaatc caggctagcc tggaacttct agtactccac
    17941 cttgcatcag gtttccaaga ttacaaaggc atgagtcacc agtcccatct agaggacgtt
    18001 tttattgttg tttgtttgtt tgagatagga ttccatgaag ttgaactcaa cctcactacg
    18061 tagttgacat gtgacttttc atcctgctgc cttctacctc ctgaatgctg gggtgatcgg
    18121 cctgtgctac tgcctagttg cctggttcat gggatactgg gatgctgggg tgctggggtg
    18181 ctggggtgct ggggtgctag ggtgctgggt gaagtcaccc agggctttgt tcatgctgag
    18241 taggcattct acacacacac acacacacac agagagagag agagagagag agagagaggc
    18301 agagagaacg agctgtggag gatttttaaa aaaacgaata tgaagcaaat caaactaata
    18361 aaatctgtaa gaagcataca aaacatttgc tggatcatta gaaaataaat agccaatctg
    18421 ctgccatgat gtctggatcc ttcccttgtc tatacaagag ggtattctcc tgcatgctca
    18481 tcggccctca aaatgaggaa gcactgtaac acaggaaagc tcatcctgag accctgctgc
    18541 agcatcaccg attgttcccc aaatataaat tctggacagg gttatattta aaatcactag
    18601 tgtgtgtatg tctatggtta agcgtgagta ccacatgcca gtacctcagg gcacatgtgg
    18661 cggtcagagg gcaactttcg ggagtcagtt ctctccttcc actgtgggct ccaggtattg
    18721 aacccaaatg ttaacatttg cattgccagc accctttcct gctgagccat ctcactagct
    18781 tttaaagata gcaagacagc tattttactt cctgtccctt ggttttgaat ggcctgtttc
    18841 cccagctcac gatctatata gccttggcag gaacaagatt acaagtaagt tgttggggtg
    18901 gaacaatagc ttagtggtta aaagccagga ctgctctttc agaggacaca ggttcaattc
    18961 ccacccctgc catggtctgg tttcagggga cctgacaccc tcatctggcc tccaaggtca
    19021 ccccatgcat gtgaaacaca gacacacatg caggcaaaac actacgaatg cagataaaat
    19081 aaatctttaa gaagtaattt ttaagttgat atctgaggat gttgcttagt agggagaata
    19141 cttttcctaa agaaaacctc agctcaattc ctcatcccac aaataaacac agaaagaaag
    19201 aaagaagata aaaaagagaa ttatgcttac aggcatatat atcagttttt gtatttgtcc
    19261 tgtgccattt aattggattg ttggaatagg gtggtacagt tgggcttctc tgtgtaaggt
    19321 tattttcctc agtctgttaa ctgtttttta actttaattt ttttttgcta ctttattaga
    19381 attaactgtt ttctaaaatg tactcaatat caagcaaata tagaagtcga tctcatactt
    19441 taaatttact actttattta taccaaaaga aatctagaca ttcctacttt aatgggatat
    19501 aatgacagta acaggttgtt tgtgtttgtt tattagtatt cagattctcc caaacccagt
    19561 cagaaacttc ctctctctct ctctctcttt ccttctctct ctctctctct ctctctctct
    19621 ccctccctcc ctccctccct ccctctctcc ctccctccct tccttccttc cttccttccc
    19681 cactcccaca cccctatttt ctagagacag ggtggtcttg ctatgcagta gtggctggca
    19741 ttcatcctta tgtacagacc agctagcctt gagctagctg agattcccct gtctctgcct
    19801 tttgagtatt aagattatgg gtgtatacca tccttcatac ttcaccaaac tgaattcttt
    19861 taaatagtgc agtttggggg ccagccatgt cagtacttgg tggttaaaag catgcactgc
    19921 ccatgtaagg gagcccggtt cagttcccag cacccacatt gggtagctca cagtcacctg
    19981 taactctagt ttcaggccat gtgacatgga tgcaaaaacc cacccgcata catacgtata
    20041 cacataatta aaaacaaaaa ttttaaatgc tggccaggta tggtggcaca cagcctttaa
    20101 tcccaaacac tcgggagaca gaggcaggtg gatctctgtg agttccaggc cagcctggtc
    20161 cacagagttt caggccagcc atggctacat ggtcttaaaa acaaaacaaa ggaaacgatc
    20221 aacaacatta aagtactgtt taactggcct ggtggtgtag gcctgcaatc caagttacct
    20281 gagaggctga ggcaggaaga gagaaacaga cagcagcccc tgcctgtgct acatggtgag
    20341 gtccaggcca acctaagtgt cttagtaaga cccagcttcc ttggtctttc tctggcagct
    20401 gggtgctttg gcggaagtct ttgcctctca gcactgctgc tccataggca tggcagaaaa
    20461 gtcactaacc ttcttccagg tccaggacat cttgaaactt aaagacaagc ccttctccct
    20521 ggtgctggag gaagatggca caatcgtgga gacagaagaa tacttccaag ccctggcaaa
    20581 agataccatg ttcatggtcc tgctgaaggg gcagaagtgg aagcccccat cagaacaggt
    20641 gacggccttc ccggggcagg ggtgaatgtc gagagctcgg gagatgttcc agaggtgagc
    20701 ttacacgggc ttgtcttgca gcgcaagaag agagcccagc tagccctttc ccagaagcca
    20761 actaagaaga tcgatgtggc ccgggtaacc ttcgacctgt acaagctgaa ccctcaggac
    20821 tttattggct gcctgaacgt gaaggcaacc ctctatgaca catactcgct ttcctatgac
    20881 ctgcactgct acaaggccaa gcgcatcgtg aagtgagtgg gttgggacac acagggaccg
    20941 gttgggagaa acgctaagac aagaggcctg acctgtctgc ccaaagcaaa actctctcag
    21001 gggctgggtg gacgctgggg ctaacaaaga atgacagccc cgtctgtgca gggatctaag
    21061 tggcttgatt tgcattaggg tatgtggggg gtgggggggg ggagtagttt taaggcttct
    21121 ttctagttta gcaaagtaca tttttttcac ccccaaacgt aaactttgca tagtagctga
    21181 ct
//