LOCUS       96091#pL1L2_GTK_nEGFPO_T2A_CreERT_puro#L3L4_pD223_DTA_T_spec        22277 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GTK_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 96091
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000102478
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            9759..9814
                     /db_xref="ENSEMBL:ENSMUSE00000661697"
                     /rank="1"
                     /note="ENSMUSE00000661697 5' fragment"
     misc_feature    4275..9814
                     /note="5 arm"
     misc_feature    9842..9866
                     /note="B1 Gateway"
     misc_feature    9879..9926
                     /note="Frt"
     misc_feature    9927..10427
                     /note="mouse En2 intron"
     primer          complement(9995..10016)
                     /note="LRPCR LAR3_EN2"
     primer          complement(9891..9914)
                     /note="FRT3"
     primer          13930..13948
                     /note="seq primer NF"
     misc_feature    13990..13997
                     /note="RE sites"
     misc_feature    15790..15823
                     /note="loxP"
     misc_feature    complement(13998..14029)
                     /note="Rox"
     misc_feature    complement(15750..15781)
                     /note="Rox"
     misc_feature    14030..14051
                     /note="polylinker"
     CDS             14610..15206
                     /note="puromycin N-acetyl transferase"
     misc_feature    14725..14730
                     /note="Mutated RsrII site"
     misc_feature    10964..11680
                     /note="EGFP"
     misc_feature    10874..10963
                     /note="3X NLS"
     misc_feature    13746..13984
                     /note="SV40 polyadenylation site"
     misc_feature    12779..13723
                     /note="ERT2"
     promoter        14062..14573
                     /note="PGK promoter"
     misc_feature    15317..15749
                     /note="PGK pA"
     primer          13804..13827
                     /note="LRPCR RAF5"
     misc_feature    11690..11743
                     /note="T2A"
     misc_feature    11740..11740
                     /note="A to G to kill RsRII site"
     misc_feature    11744..12769
                     /note="Cre"
     misc_feature    10702..10723
                     /note="seq primer FCHK2"
     misc_feature    10428..10864
                     /note="mouse En2 exon"
     misc_feature    10816..10884
                     /note="FCHK cre_ert_gtk"
     misc_feature    10816..10851
                     /note="FCHK QC prefix region"
     misc_feature    10852..10864
                     /note="FCHK QC critical region"
     misc_feature    10865..10884
                     /note="FCHK QC suffix region"
     misc_feature    15846..15866
                     /note="B2 Gateway"
     misc_feature    9815..15896
                     /note="Synthetic Cassette"
     exon            15897..16084
                     /db_xref="ENSEMBL:ENSMUSE00000661697"
                     /type="targeted"
                     /rank="1"
                     /note="target exon 1 ENSMUSE00000661697 3' fragment"
     exon            16175..16386
                     /db_xref="ENSEMBL:ENSMUSE00001238533"
                     /type="targeted"
                     /rank="2"
                     /note="target exon 2 ENSMUSE00001238533"
     exon            16480..16534
                     /db_xref="ENSEMBL:ENSMUSE00001260473"
                     /type="targeted"
                     /rank="3"
                     /note="target exon 3 ENSMUSE00001260473"
     exon            16620..16780
                     /db_xref="ENSEMBL:ENSMUSE00000109906"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 4 ENSMUSE00000109906"
     exon            16900..16983
                     /db_xref="ENSEMBL:ENSMUSE00001312558"
                     /type="targeted"
                     /rank="5"
                     /note="target exon 5 ENSMUSE00001312558"
     exon            17213..17387
                     /db_xref="ENSEMBL:ENSMUSE00001231974"
                     /type="targeted"
                     /rank="6"
                     /note="target exon 6 ENSMUSE00001231974"
     exon            17506..17705
                     /db_xref="ENSEMBL:ENSMUSE00000286043"
                     /type="targeted"
                     /rank="7"
                     /note="target exon 7 ENSMUSE00000286043"
     exon            17833..18030
                     /db_xref="ENSEMBL:ENSMUSE00000661696"
                     /type="targeted"
                     /rank="8"
                     /note="target exon 8 ENSMUSE00000661696 5' fragment"
     misc_feature    15897..18030
                     /note="Critical Region"
     misc_feature    18054..18087
                     /note="Downstream LoxP"
     misc_feature    18031..18110
                     /note="synthetic loxP region"
     exon            18111..18296
                     /db_xref="ENSEMBL:ENSMUSE00000661696"
                     /rank="8"
                     /note="ENSMUSE00000661696 3' fragment"
     misc_feature    18111..22277
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggaaatatg tatgtgaggt ccaagagtta aggaaagact tgtgagttga
     4321 gaaaagaaac ttctccaagg cctgtctatg aggccttgat catgatccag aagaaatggt
     4381 ttcccaaagc ctgaccatca ttgtcaaaca gtaaagtaag ggctagaaag tacctgttgt
     4441 gtttgaaagt aaggatgcca ggcctggaag agggtttagt gattaagagc acatactgtc
     4501 cttgaagagg acctgagttc agttcccagc acccacatct gatagccaca gctgtctgta
     4561 acgccagtct caggccgtct gacatcctct gtcctcactc atatgtacac atacacatac
     4621 ataaatgcta agaaagaaag aaagaaagga aggaaggaag gaaggaagga aggaaggatg
     4681 gatggatgga tggatggatg tcagtggcgc actactgaca aggtagtaag gtaagaatta
     4741 taggcctgcc tgccttcctc cctcctcatt aaatagaaga gaaaggacag taggcactaa
     4801 aacatttaag ggcttttctt ttaattactg ttgttttagt gggtgagact taaacacata
     4861 agaatgttca gggaaaggca ttcatgggga gctagagaga gaactgctgt aaggtagatc
     4921 gtggatgctc accctggaag gacaattcag agctcaggaa atgatctaaa gttgtaaagt
     4981 acagcggaac agtggatggt tatttttttt ttcctttgtc cccaggctga atcagagatc
     5041 attctgccca gcacaggctc tgctccagcc caggaacacc ctctgtccaa tgacagcagc
     5101 atctcagaac agaccccgac agcagcagta gatgaaggtt aacgaccttg gcatctcagg
     5161 gagcggaaag tgacctgctc ttgctctcag cagtacagat gcacactgcc agacatgctg
     5221 gtcttacccc tctctatggt gttgggtttt tgtttttgtg tttatttaag aggtagggtc
     5281 ggtgctggag agatggctca gaggttaaga gtactgactg ttcttccaga ggtcctgagt
     5341 tcaaatccca gcaaccacat ggtggctcac aaccatctgt aatgggatct gatgccctct
     5401 tctggtgttt ctgaggacag ctacagtgta cttatatata ataaatgaat cttaaagaaa
     5461 aaaaaaaaaa aagaggtctc atgtggttca ggatggccat gaatttctat gtggctaaat
     5521 aaccctgatt tgtttttgct ttagtgccag aaccagctcc tgtgccattg cttggaagtg
     5581 ttaagagtgc tttcacccga gtagcctcaa tggcttcctt tcagcctaca ggttaggatg
     5641 ggctccaggg accatccctc atttctcaaa ggtcaggtag tgatgtgcaa agacaaaagc
     5701 agtcagaagt gctttgctct aaaaacctca attctacttg ggctcatttt atttctgtct
     5761 cttgggatct gtaatgttcc ggatagttaa accatgtggg tgggaaacat aaagttgtgg
     5821 gagcagtggt tgtgtaccct gtccttgttg gcagaagggc tatgtctcca tctagaactt
     5881 tgtctttcag agaccccaga cttggagaag agcctggcag aaatgagtac tgtgttacaa
     5941 gagcttaaga gcctgtgttc cctgctgcaa gagtctaaag aggaggccac tggggtcctg
     6001 cagagggaaa tgtaagcggt gtcagtgtta atgatgggga gtgatgagag cagccctcat
     6061 gccaagggca gagcatgaga atgatgccag attctcctgc cccccctcac cccccccagc
     6121 tgtgaactac actcgagact acaggcccaa gaagaagagc atcaggaagc cctgaaggca
     6181 aaggaagcag acatggagaa gctgaaccag gccttgtgct tgctccgcaa ggtgaaggag
     6241 ggaccttaaa gtggggcaat gaggaactgg ggcagctccg ctggcccatt gggaccccct
     6301 ttctaggaat ctaggtagtg ggggggttcc aactactgct ctagttttgc ccttttgggg
     6361 tcagacactt cctatctgag aactagtact gggttgacct gtcttctcca agcagaccca
     6421 ggagaaaagc ccagaactga agcagagttc tgaaaccaag acgtgccatg cttgacttgg
     6481 tcctgaataa gtggacggat atgaacgagt ccccggggct gagcatggct cgggcattca
     6541 gtcctgtctt ctggctgaaa agaagtgagc ctttcctcag tgcttctgtc cttcttcaca
     6601 gaatgagaag gagctcctgg aagtgataca gaagcagaac gagaagatcc tggggcaaat
     6661 agacaagagc ggccagctca tagtacgttt ctcctgatgc tatggccagg tctctgcttt
     6721 tcccacctga tggaggaagg agagaaagaa cctctgagta ctgttgggtt atctccgtta
     6781 cccctgagtg ctgttgggtt acctctgctc ttgttcccag aacctcagag aggaggtgac
     6841 ccagctcaca cagtcacttc ggcgtgcaga gacagagact aaagtgctcc aggaagccct
     6901 ggaaggccag ctagatccca gctgccagct gatggctact aactggatcc aggaaaaagt
     6961 gtttctctca caggaggtaa cacagagtta acaaaggagc tgtacaaata atggggaccc
     7021 cacgctatgc ccctattaac gtggacatat ttataggtga gcaagctgag ggttatgttc
     7081 ctggagatga aaactgagaa ggaacagctg atggacaagt atctgagcca tgtaagcagt
     7141 tctgtttcct tccaccacag aagcaccctg ccatatatat ttcctctgtt ctctctagct
     7201 gtgttcttta cgtgtagggt tcatgaagcc tgtttgcatt gtagaggcac atcctggagg
     7261 agaatcttcg gcgctctgac acagagttaa agaaactcga tgacacaatt cagcatgtct
     7321 atgaggtaaa agtgaaggag gtagtgggac ctggctgccg gaggggctga ggagcatggc
     7381 tccggttctc agcctgacta cttttttttt tttttaaaga tttatttatt tattatatga
     7441 aagtacactg tagctgtcct ctgagggagt cagatctcat tacagatggt tgtgagccac
     7501 catgtggctg ctgggacttg aactcaggat cttcggaaga acaggcagtg ctcctaaccg
     7561 ctgagccatc tctccagccc tcagcctgac ttcttacagt ggcttcattt tcacttttcc
     7621 agcatcacag gccaggagct ttggcttcag gcttgtgatt cagaactgtt ttctgtctct
     7681 tcccagactc tgttgtctat cccagagact atgaagagtt gcaaggagtt acaaggattg
     7741 ctagaatttc tgagctaaga aactgaaaat attggaatgc agtggtgtgc acctgtagtt
     7801 ccagctgctc cagacgccaa agttggagtg ctgccaaatc ccaggaattt gggaacctaa
     7861 cacagacaac acagcaaaaa ccctgaaaaa tgaattccac tccactccag tttccttcag
     7921 ggctctttat cccaaccctt atcccaataa taagaccaat tggcatagag ccgaccatgt
     7981 tttatgttat ttaaataaag tgtatttaat gtagtccttc cagtccactg aatcttattt
     8041 tgggggtagg agtgggcaga tcagcacttc agcctaggca cccttcacca cttcctgctg
     8101 cagctaaagc caaggctgac tgcttttctt tgcttgattc tcttcttcta ccaacaaaaa
     8161 aagggggtgg gggggagggc aatggactgc ctgttccctg gaccactgcc tggggggaac
     8221 agaaaagact ccattcactt actttcccca tcccccttgg gggtaggcac tggtaaatat
     8281 ggggcagagc acagaaactc aattccatta ttccatgtct tgaagtgggg cactgttagg
     8341 tgggcctctg ccctacctgc tgatgtttag agatctctcc tctaaaccag gcttaatccc
     8401 caacattcca cataatgcct tacacagcaa aggactgatt gtggtagaga caaacacaag
     8461 tccatctctt ccctctaggg ccctgttggc agggttccat gatgtgggaa gggcatcgtc
     8521 tctaacaacc ttccggaccc tggcagagga atcactccac ctcactactc cacctctgag
     8581 ggcgtggcct taccctagac cagtcctggg gagagggcgg gaccaggacg ggaggtatct
     8641 gtcacgcccc cagggagtca cgtagctctg cggcaagtgc tgccacctta tttactccag
     8701 cttggaccga gctaatcaga ggaaaagtga gctgtgctgt ggctcccgcg gctgactggc
     8761 tgagtgactg gctgagtata ggtaaggtct ccaagcgagc ctgagatgct cgcgtccggt
     8821 ttgtcttccc atgctgtcct tgccctctcc ctgggaccca agttcctctg ctttctgtgg
     8881 ttttgaaacc ccatgcatgg ctcaggaaaa ccgtgcttct cttcttcctt ctagttctcc
     8941 tttccagttc caagtcatat gcctccagga ctttttcatt ggggtcagtg cattccaccc
     9001 aggacatttt aatctcagtc catttctggt tcttctaggc ttgtcctcta ctctagggta
     9061 taggcccagg aagagcatca ggaccggtta gcttgtatca ccattacccg tctcacctct
     9121 tccaggtctc cttcctgctt ttcctgcatc tctccctctt tcccataatc cactcactca
     9181 gccttaattc ctcgcctctg gggtctctac taggaggctg agttgtgggg agccaagcct
     9241 ccctatttcc tgtgcctctt gcatatgagg gaatgccggg tactagggat gatctggtga
     9301 tgtctctttc ctgtcctctc atgtatgctg atattgcctc tctcctacca tacttgcagc
     9361 cctgggagtc aatctccacc ataggtaggg gagtaggcag tagcaaaggc agtcaggttt
     9421 tcagtacctg taccactgct actccctgcc ccctgccttc cactagactt ggggaaggat
     9481 gctgtaggtt aaacgtctca ttcacaaacc tttgagaaaa gtgatccccc cccccccccg
     9541 ccttagggac ccttttgaag aaaagaaagg ggaagcagga aagcaaaaat cagaaacaac
     9601 aggttttctg tttacctcag gaaggggcca gattcagtga aagaagagct ggcattctca
     9661 acctggcagc agtttccagg aagacctgtg gcttcagggc tgtcgcctcc catcccccac
     9721 gctgcatctc ttctggagct gggaagggtg cccaccagga acaaaaggag atgtgggaac
     9781 tggggcccta actctgttag ctgtcaaaga gattaaggcg cataacgata ccacgatatc
     9841 aacaagtttg tacaaaaaag caggctggcg ccggaaccga agttcctatt ccgaagttcc
     9901 tattctctag aaagtatagg aacttcgaac cctttcccac accaccctcc acacttgccc
     9961 caaacactgc caactatgta ggaggaaggg gttgggacta acagaagaac ccgttgtggg
    10021 gaagctgttg ggagggtcac tttatgttct tgcccaaggt cagttgggtg gcctgcttct
    10081 gatgaggtgg tcccaaggtc tggggtagaa ggtgagaggg acaggccacc aaggtcagcc
    10141 ccccccccct atcccatagg agccaggtcc ctctcctgga caggaagact gaaggggaga
    10201 tgccagagac tcagtgaagc ctggggtacc ctattggagt ccttcaagga aacaaacttg
    10261 gcctcaccag gcctcagcct tggctcctcc tgggaactct actgcccttg ggatcccctt
    10321 gtagttgtgg gttacatagg aagggggacg ggattcccct tgactggcta gcctactctt
    10381 ttcttcagtc ttctccatct cctctcacct gtctctcgac cctttcccta ggatagactt
    10441 ggaaaaagat aaggggagaa aacaaatgca aacgaggcca gaaagatttt ggctgggcat
    10501 tccttccgct agcttttatt gggatcccct agtttgtgat aggcctttta gctacatctg
    10561 ccaatccatc tcattttcac acacacacac accactttcc ttctggtcag tgggcacatg
    10621 tccagcctca agtttatatc accaccccca atgcccaaca cttgtatggc cttgggcggg
    10681 tcatcccccc ccccaccccc agtatctgca acctcaagct agcttgggtg cgttggttgt
    10741 ggataagtag ctagactcca gcaaccagta acctctgccc tttctcctcc atgacaacca
    10801 ggtcccaggt cccgaaaacc aaagaagaag aacgcagatc ataactgact aggaggccac
    10861 catgggcgga ccggtgcatg tggaccccaa gaaaaagcgg aaggtggacc ctaaaaagaa
    10921 acgcaaagtg gatcctaaga aaaaaagaaa ggtgcacgtg tccatggtgt ccaagggcga
    10981 ggaactgttc accggcgtgg tgcccatcct ggtggaactg gatggcgacg tgaacggcca
    11041 caagttcagc gtgtccggcg agggcgaagg cgacgccaca tacggaaagc tgaccctgaa
    11101 gttcatctgc accaccggca agctgcccgt gccttggcct accctcgtga ccacactgac
    11161 ctacggcgtg cagtgcttca gcagataccc cgaccatatg aagcagcacg acttcttcaa
    11221 gagcgccatg cccgagggct acgtgcagga aagaaccatc ttctttaagg acgacggcaa
    11281 ctacaagacc agggccgaag tgaagttcga gggcgacacc ctcgtgaaca gaatcgagct
    11341 gaagggcatc gacttcaaag aggacggcaa catcctgggc cacaagctgg agtacaacta
    11401 caacagccac aacgtgtaca tcatggccga caagcagaaa aacggcatca aagtgaactt
    11461 caagatccgg cacaacatcg aggacggctc cgtgcagctg gccgaccact accagcagaa
    11521 cacccctatc ggcgacggcc ctgtgctgct gcctgacaac cactacctga gcacccagag
    11581 cgccctgagc aaggacccca acgagaagag ggaccacatg gtgctgctgg aattcgtgac
    11641 cgccgctggc atcaccctgg gcatggacga gctgtacaag cctgcaggcg agggcagagg
    11701 aagtcttcta acatgcggtg acgtggagga gaatcccggg ccgtccaatt tactgaccgt
    11761 acaccaaaat ttgcctgcat taccggtcga tgcaacgagt gatgaggttc gcaagaacct
    11821 gatggacatg ttcagggatc gccaggcgtt ttctgagcat acctggaaaa tgcttctgtc
    11881 cgtttgccgg tcgtgggcgg catggtgcaa gttgaataac cggaaatggt ttcccgcaga
    11941 acctgaagat gttcgcgatt atcttctata tcttcaggcg cgcggtctgg cagtaaaaac
    12001 tatccagcaa catttgggcc agctaaacat gcttcatcgt cggtctgggc tgccacgacc
    12061 aagtgacagc aatgctgttt cactggttat gcggcggatc cgaaaagaaa acgttgatgc
    12121 cggtgaacgt gcaaaacagg ctctagcgtt cgaacgcact gatttcgacc aggttcgttc
    12181 actcatggaa aatagcgacc gctgccagga tataaggaat ctggcatttc tggggattgc
    12241 ttataacacc ctgttacgta tagccgaaat tgccaggatc agggttaaag atatctcacg
    12301 tactgacggt gggagaatgt taatccatat tggcagaacg aaaacgctgg ttagcaccgc
    12361 aggtgtagag aaggcactta gcctgggggt aactaaactg gtcgagcgat ggatttccgt
    12421 ctctggtgta gctgatgatc cgaataacta cctgttttgc cgggtcagaa aaaatggtgt
    12481 tgccgcgcca tctgccacca gccagctatc aactcgcgcc ctggaaggga tttttgaagc
    12541 aactcatcga ttgatttacg gcgctaagga tgactctggt cagagatacc tggcctggtc
    12601 tggacacagt gcccgtgtcg gagccgcgcg agatatggcc cgcgctggag tttcaatacc
    12661 ggagatcatg caagctggtg gctggaccaa tgtaaatatt gtcatgaact atatccgtaa
    12721 cctggatagt gaaacagggg caatggtgcg cctgctggaa gatggcgatc tcgagccatc
    12781 tgctggagac atgagagctg ccaacctttg gccaagcccg ctcatgatca aacgctctaa
    12841 gaagaacagc ctggccttgt ccctgacggc cgaccagatg gtcagtgcct tgttggatgc
    12901 tgagcccccc atactctatt ccgagtatga tcctaccaga cccttcagtg aagcttcgat
    12961 gatgggctta ctgaccaacc tggcagacag ggagctggtt cacatgatca actgggcgaa
    13021 gagggtgcca ggctttgtgg atttgaccct ccatgatcag gtccaccttc tagaatgtgc
    13081 ctggctagag atcctgatga ttggtctcgt ctggcgctcc atggagcacc cagtgaagct
    13141 actgtttgct cctaacttgc tcttggacag gaaccaggga aaatgtgtag agggcatggt
    13201 ggagatcttc gacatgctgc tggctacatc atctcggttc cgcatgatga atctgcaggg
    13261 agaggagttt gtgtgcctca aatctattat tttgcttaat tctggagtgt acacatttct
    13321 gtccagcacc ctgaagtctc tggaagagaa ggaccatatc caccgagtcc tggacaagat
    13381 cacagacact ttgatccacc tgatggccaa ggcaggcctg accctgcagc agcagcacca
    13441 gcggctggcc cagctcctcc tcatcctctc ccacatcagg cacatgagta acaaaggcat
    13501 ggagcatctg tacagcatga agtgcaagaa cgtggtgccc ctctatgacc tgctgctgga
    13561 ggcggcggac gcccaccgcc tacatgcgcc cactagccgt ggaggggcat ccgtggagga
    13621 gacggaccaa agccacttgg ccactgcggg ctctacttca tcgcattcct tgcaaaagta
    13681 ttacatcacg ggggaggcag agggtttccc tgccacagct tgatgaagat ctgagctctt
    13741 aattaatgat cataatcagc catatcacat ctgtagaggt tttacttgct ttaaaaaacc
    13801 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
    13861 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
    13921 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
    13981 tctggatccg tttaaactaa ctttaaataa ttggcattat ttaaagttac atatggcggc
    14041 cgccctaagg ctcgacctcg aaattctacc gggtagggga ggcgcttttc ccaaggcagt
    14101 ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc
    14161 ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct
    14221 tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt
    14281 cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca
    14341 ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct
    14401 tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc
    14461 tcaggggcgg ggcgggcgcc cgaaggtctt ccggaggccc ggcattctgc acgcttcaaa
    14521 agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcatcc
    14581 atctagatct cgagcagctg aagcttacca tgaccgagta caagcccacg gtgcgcctcg
    14641 ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc
    14701 ccgccacgcg ccacaccgtc gatccagatc gccacatcga gcgggtcacc gagctgcaag
    14761 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
    14821 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
    14881 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
    14941 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct
    15001 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
    15061 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
    15121 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
    15181 ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg
    15241 aaaggagcgc acgaccccat gcatcgatga tatcagatcc ccgggatgca gaaattgatg
    15301 atctattaaa caataaagat gtccactaaa atggaagttt ttcctgtcat actttgttaa
    15361 gaagggtgag aacagagtac ctacattttg aatggaagga ttggagctac gggggtgggg
    15421 gtggggtggg attagataaa tgcntgctct ttactgaagg ctctttacta ttgctttatg
    15481 ataatgtttc atagttggat atcataattt aaacaagcaa aaccaaatta agggccagct
    15541 cattcctccc actcatgatc tatagatcta tagatctctc gtgggatcat tgtttttctc
    15601 ttgattccca ctttgtggtt ctaagtactg tggtttccaa atgtgtcagt ttcatagcct
    15661 gaagaacgag atcagcagcc tctgttccac atacacttca ttctcagtat tgttttgcca
    15721 agttctaatt ccatcagaag ctggtcgagt aactttaaat aattggcatt atttaaagtt
    15781 aggcgcgcca taacttcgta tagcatacat tatacgaagt tatgtcgaga tatttaaata
    15841 ttagacccag ctttcttgta caaagtggtt gatatctcta tagtcgcagt aggcggtgtt
    15901 tggttaggag aggaagtaga aggtcctcgg agtctagagc ttatgtcttc tccattctag
    15961 acaactgtca tcatgcccca ctcataccca gctctttctg ctgagcagaa gaaggagttg
    16021 tcggatattg ctctacggat cgtgaccccc ggcaagggca ttctggctgc agatgagtcc
    16081 gtaggtaagt gggagcctgt ggtcagggtt gaggagaggt tgccaaggaa ttctagccaa
    16141 agaaccagcc tgtctcccct ttgcttgctt ctaggcagca tggccaaaag gctgagccaa
    16201 attggggtgg agaacactga ggagaatcgc cggctgtacc gccaggtcct attcagtgct
    16261 gatgaccgtg tgaaaaagtg cattgggggg gtcatcttct tccatgagac actctaccag
    16321 aaagatgaca atggtgtccc cttcgtccgc accatccagg ataagggcat tctcgtaggc
    16381 atcaaggtac acagtccctg gtggtgcctg ggggctaggt gtggaaacca gtgtatataa
    16441 gagggtgaca agccagacct ctatctgttt cccttgcagg ttgacaaggg tgtagtgcct
    16501 ctagctggga ccgacgggga aaccaccact caaggtacct gttgggtggg tacaggggtg
    16561 ggacatgttg atgctggcaa gatggaggca gggtggtaag gctggcactg tgttttcagg
    16621 gctggatggg ctcttggaac gctgtgctca gtataagaag gacggtgctg attttgccaa
    16681 atggcgctgt gtactaaaaa tcagtgatcg cacgccgtcg gcactggcca tattggagaa
    16741 tgccaacgtg ctggcccgct atgccagcat ctgccagcag gtgtgtgctg gggggttggg
    16801 tggttggatg agggccccgg ggccggtgga gatgtgcttt catggctttc tcccatccta
    16861 tcttctactt cttccagtta accctgctct tgctttcaga atgggatcgt gcctattgtg
    16921 gagcctgaga ttctgcctga cggagaccat gacctcaaac gttgccagta tgttacagag
    16981 aaggtaagac caaacatagc cgggagggac agcctagcag gggtgcctgt accccatccc
    17041 tgctaagatc caggcacact ttcccttaag gatctttact gatacccaaa cgggcttcat
    17101 agactaagtc tatgctgagc ccttggtttc ctgtacccac ctacccctgg gtgaggagca
    17161 cagtgcgcta ggcagtggtc ccagccctgt acaaacctcc tctgctcccc aggtcctggc
    17221 tgctgtatac aaggccctga gtgaccatca tgtatacctc gaagggactc tgctcaagcc
    17281 caatatggtg acccctggcc atgcctgtcc catcaagtat agcccagaag agatcgccat
    17341 ggcaactgtc actgccctgc gtcgtactgt gcccccagct gtcccaggta ctaaactgct
    17401 cctatccctt caggtctgac ttgagggttt tccatgacac cggggtcctt taccctgggc
    17461 aagacagaga atgatcagtc agtctgtctt ctccttctac tccaggggtg actttcctgt
    17521 ctgggggtca gagtgaagag gaggcttctc tcaacctcaa tgccatcaac cgctgcccac
    17581 ttccccggcc ctgggccctc accttctcct atgggcgtgc cctgcaggca tctgcactca
    17641 atgcctggag aggacaaagg gataatgctg gggctgctac tgaggagttt atcaagcggg
    17701 cagaggtagg gagctagagg tgatgacttg gggagggatt ctgtggacca cagaggtccc
    17761 aaaccgggca gcgtgcattc cacatccgtg gtctggctca gtctgctcat tgaatcccat
    17821 tccgttttgc agatgaacgg gcttgcagcc cagggcagat atgaaggcag tggagatggc
    17881 ggagcggcag cacagtccct ctacatcgcc aaccatgcct actgaggcac gctgactcta
    17941 cccacagccc ttggcctagc aatgcacatc ccattttgcc tatagtcaca gccagggcca
    18001 aatagctatg cagaacagag atatctttat gagatggcgc aacgcaatta atgataactt
    18061 cgtatagcat acattatacg aagttatggt ctgagctcgc catcagttca agaacagaac
    18121 atgtggacct ttttcctgtc tccaccagcc cttacagtta cccttgatgg tatagtttgt
    18181 ccttgggctc tgcttgctaa ttttctctct tgggatcagg gggacgtgaa ctccagccct
    18241 gactcgtgct ccttatgctc tgagtacata gcaaataaat gagagcaaaa cacagcacac
    18301 cgctctgtct gtctgtctgt tgcacatacc aaagtctacc tccatgtgtg cgacctcttg
    18361 ctgatcctat ctcagagccc ctctgattgt ggtggtggtg gtgaggtgag atctgagttt
    18421 tcctgagctt caagttttgt ggaggaaggg ttcacctggc tcaccatccc tagctcaggc
    18481 tcctacctgc tgatttccaa agctggagtt gttgttagag ggcctggggg tggagaggag
    18541 ggtacaggtc ttccagggac tgacacatta aaggatccag caggatgaga aactccattt
    18601 tgtgcaaggc tcaaagggga caagcaggga tcagagtggg ccagttctcc tccatctctg
    18661 aagaatgtgc acttaggtct tttccaataa ggaaaccttc caagcctcgg ggctacagag
    18721 ctgagtgtct agaagcttgc caagggctat gacccacttc catgtagaga agggattcat
    18781 cggagggttc tggacttgtt agaactgata gggctttgtg gtgaatgatt acccctgggc
    18841 tagagtgtga ggtagggtga cactagacag atgtgcactg gaaaagtcat tgatgttctg
    18901 ctctgtgtgg cctgacctta taatcaaagc agctaactca gcaatttcca tatgcttctg
    18961 ccaggcactg ttcttctaac cattattata tgcattaaca cagctctgta aagtaggtac
    19021 cattgtactt ttcataggag gaaacaggct tcaaagagag gctgagaggc tatttaggat
    19081 tcgaacagca ggcattacta tatagaggct gtgctcagga atttttcaat gaagcatcct
    19141 caatcgctcg aattggaatc gatttttaaa tcctagtcta tagtctcggc tagttgggag
    19201 gctgaggtat cagacattag ggacaggatc actttaaccc tctggatcgt atagggagat
    19261 ctgtctcaac attaactaaa ataaaattaa tcacatagta gccaaccatg ctaaaaagtt
    19321 accgttttgt cagttttatc tttaagcttt gaagagctgg tgctctgaag aaaagaccta
    19381 tgttagaaat gacattaggg gctggtgaga tggcgcaggg ggtaagagca cccgactgct
    19441 cttccaaagg tccgaagttc aaatcccagc aaccacatgg tggctcacaa ccacctgtaa
    19501 cagagatctg atgccctctt ctggtacgtc tgaagacagc tacagtgtac tcacatataa
    19561 taaataaata aatctttaaa agaagaaaaa aaaaaaaaag aaatgacatt agaaaacttt
    19621 tcgggagtca ggttcttgat aagctcgact tcatttaagt aaatattccc cttccgctga
    19681 acgtggtggt gcatgtaatc ccctgcattg gagagggccc agagtccgag gccagcctgg
    19741 gctacatgga gattatcgcc ttctctaaac tggcgttgta gagctaaaca gtaccaggtg
    19801 cactcaatga aaagccaggg tcagattgaa agtgtagccg agcgcgtacc ctctcacggg
    19861 ctccgcctgc gcactggcgt gcgtcagcag tgttgctcgt gattggcagc ggtcgcgcac
    19921 cgtggtcccg ccccctcatt ctggagtttc cgattggaga aagctcatga ggccccgccc
    19981 catcggcgac ctgggcagcc ggaagggacc atggcggccg caggagctgc ggccacggac
    20041 ctaggtgcgg tggatttcag gtggagggcg cagtggggag gtctcgcggg cagaatgtgt
    20101 gggtggggcg cgagaagcct gacagctccc tccagcctgg ggttgggggt ccgccacgcg
    20161 ggagcggctt gctcggggtt cccggccgcc cgcactcctc cacgatcccg gtgtggcggg
    20221 catcgcaccc tagttcccgc gtcttagtcg gccctctgtg ccctcagagg tggtccgggg
    20281 caagcgttcc gcgcttttct tcgccgcggt ggccatcctt ctgggactgc cgctctggtg
    20341 gaagacgacg gagacttacc gggccccatt gccttactca gacatcagtg ggctgaatgc
    20401 tctgctggtg agactgggtg gggagaaccg gggctggggg accggggggc agcccagctg
    20461 taaggtgcag acattgctaa agaccccgat gcccctcatc cctcgcgcag ctccggctca
    20521 tggtgccagt cacagttgtg tttactcggg actctgtgcc cctggacgac caagagaagc
    20581 tacctttcac cgttgtgcat gagcgagaga tccctctgaa atgtgagttc ccatgaagag
    20641 gtgtcagggc tgctttttcc atctgagcca ggcccaaaac ccagagttgg aatgcatagg
    20701 aggggcgagg gcgactcata acttacttta aaatgtttta ttacatttac ttagtgtgcg
    20761 cgcgcacatg tatgtttgtg tggatgtctg tgctgtggag catcttgtgg aggtcagagg
    20821 atcacttgcg aaagtcattt ctcctcccac catatgggtc ctggtcttgg ggatccaact
    20881 caggtcatca ggcttggtat caagagccat ccgcctgctt cccccaaatc ctaagaaaag
    20941 agatctcctt tggaatgttc acttgggggt ttgtgttagt ctaaagtaca gtgcctgtcc
    21001 ttatgacagt gatttatgtc atacggtaaa ttatgccacc ttatccttag caattgatac
    21061 ccatcagtgc atagggaaag caggttgtag ctgaaagaaa acgggagttt gaagttacat
    21121 cggatgtgta ttcagacctt tgtcatttta acatcacaca ctgtattgtt aggcactttg
    21181 ttaggtacca agaacttgag ggtgaataat aaattggtaa tattttaaag atgtttatag
    21241 ttaacatcag aaagaaagaa actagcaaaa tactgtaagg caggaggttt ggtctcgtgg
    21301 tggagtcacg ggagagcctg aactccattc tcacctgccg gggagtagaa aaaggtgcag
    21361 aagggcttgg gcaatgtgat ttttgaggtt gggtgtttct ggctttttga gacagggttt
    21421 ctttgggtag ccctggctgt cctgggtctt gctgtgtaga ccagacttgc accacacctg
    21481 gcaaggactc tgatctttat tggatgcata atctcatgtt cagttttgtt ttttcatcag
    21541 tatttttttt ttttttttac taaatgattt aggttttatt tcggtttcat gtgttctttt
    21601 ttttttttta ttttaaatta cgtattttct tcaattacat ttccaatgct atcccaaaag
    21661 tacccccccc ctactcccct acccgcccat tcccattttt tggccctggc attcccctgt
    21721 actggggcat ataacgtttg cctgaccaat gggcctctct ttccagtgat ggccgactag
    21781 gtcatctttt gatacatatg cagctagagt caagagctct ggggtactgg ttagttcata
    21841 atgttgttcc tccgataggg ttgcagatct ctttagctcc ttggatactt tctctagctc
    21901 ctccattggg ggccctgtga tccatccaat agttgactgt gagcatccac ttctgtgttt
    21961 gctaggcccc ggcctagtct cacaagggac agctatatca ccgtccttgc aacaaaggct
    22021 tgctagtgta tgcaattttt tttttttttt tttgatgtta gaatgtgatc tttggttcta
    22081 gaattaaaat aacaatttga gagtcatcaa ggacctttta taattccctc gcttgcttct
    22141 cagcagcagc tgctaacagg acacagcttc cccggcactc cttcaagcac cctttgtttt
    22201 ttgaagcagc agcccctggt gtttagaacg attcctttct agctcactct ttaaatcact
    22261 ttagccacct gtctggt
//