LOCUS       KO-first_condition_ready_125350:125517_MGI:99779        21429 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Chrna7 targeting project(s): 125350:125517
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT0_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 184161
COMMENT     backbone: L3L4_pD223_DTA_T_spec
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     misc_feature    4275..10228
                     /note="5 arm"
     misc_feature    10256..10280
                     /note="B1 Gateway"
     misc_feature    10293..10340
                     /note="Frt"
     misc_feature    10341..10840
                     /note="mouse En2 intron"
     primer          complement(10409..10430)
                     /note="LRPCR LAR3_EN2"
     primer          complement(10305..10328)
                     /note="FRT3"
     primer          14492..14510
                     /note="seq primer NF"
     misc_feature    14552..14559
                     /note="RE sites"
     misc_feature    16352..16385
                     /note="loxP"
     misc_feature    complement(14560..14591)
                     /note="Rox"
     misc_feature    complement(16312..16343)
                     /note="Rox"
     misc_feature    14592..14613
                     /note="polylinker"
     CDS             15172..15768
                     /note="puromycin N-acetyl transferase"
     misc_feature    15287..15292
                     /note="Mutated RsrII site"
     intron          10841..11215
                     /note="mouse En2 intron"
     misc_feature    11213..11215
                     /note="Splice Acceptor"
     exon            11216..11255
                     /note="mouse En2 exon"
     primer          11116..11137
                     /note="seq primer FCHK2"
     misc_feature    11256..11378
                     /note="1Dleader"
     misc_feature    11379..11430
                     /note="F2A"
     misc_feature    11526..12242
                     /note="EGFP"
     misc_feature    11436..11525
                     /note="3X NLS"
     misc_feature    14308..14546
                     /note="SV40 polyadenylation site"
     misc_feature    13341..14285
                     /note="ERT2"
     promoter        14624..15135
                     /note="PGK promoter"
     misc_feature    15879..16311
                     /note="PGK pA"
     primer          14366..14389
                     /note="LRPCR RAF5"
     misc_feature    12252..12305
                     /note="T2A"
     misc_feature    12302..12302
                     /note=" A to G to kill RsRII site"
     misc_feature    12306..13331
                     /note="Cre"
     misc_feature    11231..11256
                     /note="FCHK lf2a_lacz_gt0"
     misc_feature    11231..11246
                     /note="FCHK QC prefix region"
     misc_feature    11247..11250
                     /note="FCHK QC critical region"
     misc_feature    11251..11256
                     /note="FCHK QC suffix region"
     misc_feature    16408..16428
                     /note="B2 Gateway"
     misc_feature    10229..16458
                     /note="Synthetic Cassette"
     exon            16845..16954
                     /db_xref="ENSEMBL:ENSMUSE00000501126"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 1 ENSMUSE00000501126"
     misc_feature    16459..17143
                     /note="Critical Region"
     misc_feature    17167..17200
                     /note="Downstream LoxP"
     misc_feature    17144..17223
                     /note="synthetic loxP region"
     misc_feature    17224..21429
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggaatttca ctttttaaat cccacttact tattgaactc accactgaga
     4321 gtgcctactt tgtagagggc attggccaga gctgtgggag agcacttaga aatgtattag
     4381 ccacatttcc tctttcagcc ttttacaatc aggctgtgca attaactcat ttaaagaaag
     4441 gttaagcacc aagagatgat aaatgtttga ggagatagga tgctaattac cctgatttga
     4501 tcattataca ttgatatata catgcactat attagcatcc tgtatgtata aataagtgca
     4561 gttgggtgtt acttgatttc taatgaatca caaaaagtaa aattggtcat tatagcaaat
     4621 ctcgatgttt ctctggaata ttgtaagaat gggtgggtat acagtggggg tcacaggtgg
     4681 gaacaatcct gctttatcaa aatggaaaac agagtggcct gactcttcta aagagaagca
     4741 gtacaatgat ggcactactg tgtgcccctg cattagggta gcctacccag gtaagccctg
     4801 tcctaggcag cttgtgctag gaggagactg agtgttgaca ctgatctgga aagtgacacc
     4861 tcagttggtg gagggctagg ccactgcctc cccactgcat tttcaaagac ccccaagaat
     4921 ctttggttta aaacaattat aagcagttgc cattgaaaac ccacgtgact gtgcccttgc
     4981 ctcaacctcc attgctttga gtaggtcctg gttgcagggt ggacactgga ccttgcatgt
     5041 caccaagggc aggcgtagga gcttccttgg gacaattgat aaacaaccgt gcactgattt
     5101 ttatttctgt gctccttggc cactcacctg cctggcctga cccagccttg atattacaca
     5161 tagataagaa tgaagctcag gatggaagga agcagggcca gagttcccag gggagcccca
     5221 gcgagggcag gaggcagatg gattgtcagg gaatggcttt tagcactggg tctcagttcc
     5281 caaagcctcg tttcactaag ctctgttctg aaatctgata cacataatct tttctccagg
     5341 gtgtttctac tctgcagtca attttctgca aattatagat ttttcagtcc agtcaaaatg
     5401 gcagagtcac aaaagttttc taaaaattaa attccccttg acctacccct tgcgaatttt
     5461 cttatccttt tccccagctg tctttcattt tgcatgattg agattattcc gacagcctga
     5521 agaataccga gttgctgatt tctgagagtc tcttcctacc ctggggttgg ggtgcggaag
     5581 ctggtgggag attccttctg cagaggtttt atcattttgg gagactgtta gacatctttg
     5641 atctcatgtg tgtgaggata tgacagagga catttcagct tccacagaaa cactgggacc
     5701 taccagatgc acacattttc tttgtaaaac cctccttttc ttttttagaa catcagccat
     5761 tttgccttca gatttgatca tctttcagta cttactctct atttttagac tcctaattct
     5821 aattatagta ataatagcat tcacatcaca gattattaga ttgtaaacca taattagtcc
     5881 taaactgctt cctttttaaa tttctgcatt tattatctgt ggagcagaaa taccactcat
     5941 tgtgtttaca cattagccag cagtgtgctg ctgtgggctg gactgcagct gtgaccaggt
     6001 gagtggcact gactgtcaga gacagcaaaa tgtcagcagg aaaagcaggg gagggactca
     6061 cattcttcct ttgagtcaaa atacacgatt aggtgaattt ttaccaaaac tgggtgatgt
     6121 ttttctgaca ccaattattt gatgtcattt ttaatactta cagcaactct ctaacccagc
     6181 tggatatcca tttaatttga ttttgatatt gtccaccttg agttaatatc tgaatccaca
     6241 agctcaaagt ctgcttccca cgaggttgtt cctacatcaa aaacatgttg caaacgtgtt
     6301 gccctggaga cacatacccg tcacactgcc tgtaacccaa aggttctaag ctttggctat
     6361 caggtacaca tttactggtg ttttctctaa aggatactgc tcagaggcag atgtggaaga
     6421 ggcattgggg caggtgttga gggagaagtt accctgctcc tcaggacatg ctatacttcc
     6481 ttccatgcac tcatgtgtgt gcgctcacct ggaacgtcac tgcgctttgt acagaagttt
     6541 gtctccctca gtctccaatc tcatctccca actctagact tgaggatgag atggagtttg
     6601 tcatccctgc tctctaatca ggcagtctgg ctttcttcct gaaactgtga aaggagatcc
     6661 aaccccaaag ctgctaaatt ggcataaact tagatatggt ccaaaggatg attttagcaa
     6721 aagatattcc tgtcactcag taagtttcaa gagtttaaag agctctatac aaagaaaacc
     6781 aaatatattt ctcattacat tgcaatatat tttgaaagga aacaaaacta ttttcgttct
     6841 aaaagatttt tactttttga gaatttcatg cattagtgtt gtgtatttat atcatttcaa
     6901 actctaactc ctccaacttt tcctgtgtcc ttatccactc ctcttcaaat tattgaccac
     6961 ttatacttta attattacaa atacacagac ataccaaaga gacagggaga gtgaaggagc
     7021 gggagagggg gagggagagg atgcacacat gcgaacctta caaggttcca ttcattgttg
     7081 ctcgtttgta tatgtgtgtt tagggctgac cacaggagat taggtaacct atcgggtggc
     7141 tgttgcctgg agaagattga gttttctcta tgtagaatgg ggctctgtga gatttcccta
     7201 tctatgttgg catgtgagtg agtggtgtaa atacgtctgc tctgtcttct gtgatgttcc
     7261 atgagcctta gatctaggga ttgtgttgca ggtgaatcag ctggggttag aaacactgca
     7321 ttttggctgg tttctctagt gatctctgtc tacttcataa agaaggttat ttgatgaagg
     7381 atgagagcta cacttatctt tgagtataag tatttagaat gcaactagaa agggtgccag
     7441 tttagacaag tggcagtggt aggttctttt ttagggtcta ctctctccta gtcattgggt
     7501 agctggctag atttacagta ccaggtatga ttctcctcct attgaattag tctaattaga
     7561 gagcctgtgg ttactcccga gatatgagtg ccactatttt accttcaggg ctgtcttgcc
     7621 ttgctggtta ttgttttggt tcctaggctc taaagctggg taggactatt gactgctttg
     7681 agttcttggt agtcttcata gcaccttcat atatcatgag agctaggcat taaagagaca
     7741 gcttccaggc tgcttctatc tcaattcctc taagtcccat gaccaaccaa agtgtgtggt
     7801 atcttcagaa ttaaatttta ccttcaagtt cttggaggca accaagagca acatcaataa
     7861 tctatattgt tttgggcatt cttagactcc tttgacaaag aactggaaga gaggatattt
     7921 cacctgctct gtccactcct gtttttcatg ccatgtacac acatacaaca cacacacaca
     7981 cataccccaa atttaattaa aagttccctg aaaactcaag acatgtttat agagggccca
     8041 agtgttggat tgtataaggg tctgcagcac aatggtatat gtcatacatt ctatattacc
     8101 acctcccctt tccttctgtc accttacaac ccacatactc ttcattctat ggctgtaaag
     8161 gtatatctct gtttggttca ttcatgtggt ttctcagggg ctgccatttt gaaaggttgc
     8221 taaatgctct cgatgaagag atatgccttg ggcttctatc cccattgacc caagatctaa
     8281 gtggcaggaa acgatttgag aacacacatc catttcttgt aaagcctgac tgtaaaaacc
     8341 tcattctaca ttttttctag gaagttcccc ataggctcct atgttgtgtt agtgttccct
     8401 actacaccca tcatgctttt tatacccttg tttgtacctc aggtattaga catgctgtag
     8461 ccctgtatca tgtagtgttc tataaagtga acatattgtt ttctaaatct atcatttgtt
     8521 gcttgcctat aagaactgca tgattttgcc ctgctactga aagcatggct tcattttatc
     8581 ctaattttat catgagggga tgtgtacaca tggggaattg cagtgcagag gagataattc
     8641 agcctcctgc attctcacag caaatctcac agtccagatg tgtctggttc taaggtttct
     8701 tgtagccatt cctccccact acttgctagg tcaggttggt ttccacatta gagaaaagga
     8761 gcatgcacag atcagcacct tcagagactg catccagtct gagttgttat ggctgaaggt
     8821 ttgtagtaca gacattgctt tagaagaacc aatcctgagg cagtcagaga tttcttctat
     8881 gtcaccacat tgacaagctt ggattgtggt gaggcagaag gcacacatac agtgctgacc
     8941 aggcagacta gcaatgatgt cacctgccac aaagcaatgg agcacccaca gttgaatgtg
     9001 ctcagaagct aggagatgtg gggaaagatg ggaggtgtga ggcagctctg atatgctaag
     9061 tggtcttatg tttgaacagc cagatgatct tattccctga tatccccact catctgccta
     9121 tcatgtcatc aggcttacga gatgggtcct ttccctcatt gccagtgtgc cttcaagggt
     9181 tgttcttgga accccagtcc ctatgggtac aatattggtc accgtgatta actcagagca
     9241 acactccagg gttaagaata agatggaagt gctttgacac ggaatcaggg gagagaacag
     9301 aaccaagaac atccagatgg aacaggctgt tacaaacatg gttcagcaaa ccacttgaat
     9361 gaggcagaag ttccttagtg tttcgcctcc cctcattaat ctcatcagct gtggacccta
     9421 caggacagat gttgatgtgc cagtaatgct gctgcatttt aacatccttg tttgctgagg
     9481 tcccagttgt gagtagcggt gttctctgac ttcagattat tccacttctg cagtagactt
     9541 ttagaacact gtgggttctc caggggacct ttgagacctg tccatggatt tgtgttgcca
     9601 gaatcagtag ccgtagaccc acaacattca tctgtatatt ccaagtcaag tcgctctaac
     9661 aattttgttc cttccacttg agtattgttt cataacttta cgacaaacac aattggtcag
     9721 aaatcaaatg gtgattgcta tacatctgaa gcatttatat gccttttagc ataaatttgt
     9781 ggaaaagctt cagggcccaa atgagaatgt gtttgattat gggggggggg cactctggtt
     9841 ctagatgaag atatagcact ccaaaccttc ctaaatcctg gagagtctgg atcttgacgc
     9901 atggatgtca gtagtgacac tttagaggtg gtgggtcctt tgctgccagg cctggaatct
     9961 aaggcttgat tgcctgtcag tagtgggagg actctatggt tatatctctg ccatcttggt
    10021 cctgggaatg gtacccgatt atcagagaaa ggactgtagg tctttctggt gttggagatc
    10081 actcagaaag gcctgaccct gagttgctaa actcatcact gtactcatga gtgctcctaa
    10141 gtcttcatcc ctatgtctgt gttctgctgc tgaggctcag ggtttgaggc atcagcttat
    10201 agtatcaaaa gcactgtcta taaggaaaaa ggcgcataac gataccacga tatcaacaag
    10261 tttgtacaaa aaagcaggct ggcgccggaa ccgaagttcc tattccgaag ttcctattct
    10321 ctagaaagta taggaacttc gaaccctttc ccacaccacc ctccacactt gccccaaaca
    10381 ctgccaacta tgtaggagga aggggttggg actaacagaa gaacccgttg tggggaagct
    10441 gttgggaggg tcactttatg ttcttgccca aggtcagttg ggtggcctgc ttctgatgag
    10501 gtggtcccaa ggtctggggt agaaggtgag agggacaggc caccaaggtc agcccccccc
    10561 ccctatccca taggagccag gtccctctcc tggacaggaa gactgaaggg gagatgccag
    10621 agactcagtg aagcctgggg taccctattg gagtccttca aggaaacaaa cttggcctca
    10681 ccaggcctca gccttggctc ctcctgggaa ctctactgcc cttgggatcc ccttgtagtt
    10741 gtgggttaca taggaagggg gacgggattc cccttgactg gctagcctac tcttttcttc
    10801 agtcttctcc atctcctctc acctgtctct cgaccctttc cctaggatag acttggaaaa
    10861 agataagggg agaaaacaaa tgcaaacgag gccagaaaga ttttggctgg gcattccttc
    10921 cgctagcttt tattgggatc ccctagtttg tgataggcct tttagctaca tctgccaatc
    10981 catctcattt tcacacacac acacaccact ttccttctgg tcagtgggca catgtccagc
    11041 ctcaagttta tatcaccacc cccaatgccc aacacttgta tggccttggg cgggtcatcc
    11101 ccccccccac ccccagtatc tgcaacctca agctagcttg ggtgcgttgg ttgtggataa
    11161 gtagctagac tccagcaacc agtaacctct gccctttctc ctccatgaca accaggctcc
    11221 caggtcccga aaaccaaaga agaagaacgc agatctctag agtcaccgag ttgctttacc
    11281 ggatgaagag ggccgaaaca tactgtccaa ggcccttgct ggcaatccac ccaactgaag
    11341 ccagacacaa acagaaaatt gtggcaccgg tgaaacagac tttgaatttt gaccttctca
    11401 agttggcggg agacgtcgag tccaaccccg gaccggtgca tgtggacccc aagaaaaagc
    11461 ggaaggtgga ccctaaaaag aaacgcaaag tggatcctaa gaaaaaaaga aaggtgcacg
    11521 tgtccatggt gtccaagggc gaggaactgt tcaccggcgt ggtgcccatc ctggtggaac
    11581 tggatggcga cgtgaacggc cacaagttca gcgtgtccgg cgagggcgaa ggcgacgcca
    11641 catacggaaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccttggc
    11701 ctaccctcgt gaccacactg acctacggcg tgcagtgctt cagcagatac cccgaccata
    11761 tgaagcagca cgacttcttc aagagcgcca tgcccgaggg ctacgtgcag gaaagaacca
    11821 tcttctttaa ggacgacggc aactacaaga ccagggccga agtgaagttc gagggcgaca
    11881 ccctcgtgaa cagaatcgag ctgaagggca tcgacttcaa agaggacggc aacatcctgg
    11941 gccacaagct ggagtacaac tacaacagcc acaacgtgta catcatggcc gacaagcaga
    12001 aaaacggcat caaagtgaac ttcaagatcc ggcacaacat cgaggacggc tccgtgcagc
    12061 tggccgacca ctaccagcag aacaccccta tcggcgacgg ccctgtgctg ctgcctgaca
    12121 accactacct gagcacccag agcgccctga gcaaggaccc caacgagaag agggaccaca
    12181 tggtgctgct ggaattcgtg accgccgctg gcatcaccct gggcatggac gagctgtaca
    12241 agcctgcagg cgagggcaga ggaagtcttc taacatgcgg tgacgtggag gagaatcccg
    12301 ggccgtccaa tttactgacc gtacaccaaa atttgcctgc attaccggtc gatgcaacga
    12361 gtgatgaggt tcgcaagaac ctgatggaca tgttcaggga tcgccaggcg ttttctgagc
    12421 atacctggaa aatgcttctg tccgtttgcc ggtcgtgggc ggcatggtgc aagttgaata
    12481 accggaaatg gtttcccgca gaacctgaag atgttcgcga ttatcttcta tatcttcagg
    12541 cgcgcggtct ggcagtaaaa actatccagc aacatttggg ccagctaaac atgcttcatc
    12601 gtcggtctgg gctgccacga ccaagtgaca gcaatgctgt ttcactggtt atgcggcgga
    12661 tccgaaaaga aaacgttgat gccggtgaac gtgcaaaaca ggctctagcg ttcgaacgca
    12721 ctgatttcga ccaggttcgt tcactcatgg aaaatagcga ccgctgccag gatataagga
    12781 atctggcatt tctggggatt gcttataaca ccctgttacg tatagccgaa attgccagga
    12841 tcagggttaa agatatctca cgtactgacg gtgggagaat gttaatccat attggcagaa
    12901 cgaaaacgct ggttagcacc gcaggtgtag agaaggcact tagcctgggg gtaactaaac
    12961 tggtcgagcg atggatttcc gtctctggtg tagctgatga tccgaataac tacctgtttt
    13021 gccgggtcag aaaaaatggt gttgccgcgc catctgccac cagccagcta tcaactcgcg
    13081 ccctggaagg gatttttgaa gcaactcatc gattgattta cggcgctaag gatgactctg
    13141 gtcagagata cctggcctgg tctggacaca gtgcccgtgt cggagccgcg cgagatatgg
    13201 cccgcgctgg agtttcaata ccggagatca tgcaagctgg tggctggacc aatgtaaata
    13261 ttgtcatgaa ctatatccgt aacctggata gtgaaacagg ggcaatggtg cgcctgctgg
    13321 aagatggcga tctcgagcca tctgctggag acatgagagc tgccaacctt tggccaagcc
    13381 cgctcatgat caaacgctct aagaagaaca gcctggcctt gtccctgacg gccgaccaga
    13441 tggtcagtgc cttgttggat gctgagcccc ccatactcta ttccgagtat gatcctacca
    13501 gacccttcag tgaagcttcg atgatgggct tactgaccaa cctggcagac agggagctgg
    13561 ttcacatgat caactgggcg aagagggtgc caggctttgt ggatttgacc ctccatgatc
    13621 aggtccacct tctagaatgt gcctggctag agatcctgat gattggtctc gtctggcgct
    13681 ccatggagca cccagtgaag ctactgtttg ctcctaactt gctcttggac aggaaccagg
    13741 gaaaatgtgt agagggcatg gtggagatct tcgacatgct gctggctaca tcatctcggt
    13801 tccgcatgat gaatctgcag ggagaggagt ttgtgtgcct caaatctatt attttgctta
    13861 attctggagt gtacacattt ctgtccagca ccctgaagtc tctggaagag aaggaccata
    13921 tccaccgagt cctggacaag atcacagaca ctttgatcca cctgatggcc aaggcaggcc
    13981 tgaccctgca gcagcagcac cagcggctgg cccagctcct cctcatcctc tcccacatca
    14041 ggcacatgag taacaaaggc atggagcatc tgtacagcat gaagtgcaag aacgtggtgc
    14101 ccctctatga cctgctgctg gaggcggcgg acgcccaccg cctacatgcg cccactagcc
    14161 gtggaggggc atccgtggag gagacggacc aaagccactt ggccactgcg ggctctactt
    14221 catcgcattc cttgcaaaag tattacatca cgggggaggc agagggtttc cctgccacag
    14281 cttgatgaag atctgagctc ttaattaatg atcataatca gccatatcac atctgtagag
    14341 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
    14401 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
    14461 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
    14521 ctcatcaatg tatcttatca tgtctggatc cgtttaaact aactttaaat aattggcatt
    14581 atttaaagtt acatatggcg gccgccctaa ggctcgacct cgaaattcta ccgggtaggg
    14641 gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg
    14701 cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg
    14761 gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc
    14821 ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact
    14881 agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc
    14941 ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc
    15001 gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ttccggaggc
    15061 ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct
    15121 ccgggccttt cgacctgcat ccatctagat ctcgagcagc tgaagcttac catgaccgag
    15181 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
    15241 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccaga tcgccacatc
    15301 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
    15361 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa
    15421 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
    15481 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
    15541 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
    15601 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
    15661 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
    15721 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc
    15781 cacgacccgc agcgcccgac cgaaaggagc gcacgacccc atgcatcgat gatatcagat
    15841 ccccgggatg cagaaattga tgatctatta aacaataaag atgtccacta aaatggaagt
    15901 ttttcctgtc atactttgtt aagaagggtg agaacagagt acctacattt tgaatggaag
    15961 gattggagct acgggggtgg gggtggggtg ggattagata aatgcntgct ctttactgaa
    16021 ggctctttac tattgcttta tgataatgtt tcatagttgg atatcataat ttaaacaagc
    16081 aaaaccaaat taagggccag ctcattcctc ccactcatga tctatagatc tatagatctc
    16141 tcgtgggatc attgtttttc tcttgattcc cactttgtgg ttctaagtac tgtggtttcc
    16201 aaatgtgtca gtttcatagc ctgaagaacg agatcagcag cctctgttcc acatacactt
    16261 cattctcagt attgttttgc caagttctaa ttccatcaga agctggtcga gtaactttaa
    16321 ataattggca ttatttaaag ttaggcgcgc cataacttcg tatagcatac attatacgaa
    16381 gttatgtcga gatatttaaa tattagaccc agctttcttg tacaaagtgg ttgatatctc
    16441 tatagtcgca gtaggcggtg taataaatga tttttttttt tcaaaatgcc ctttcagaga
    16501 aaacttggaa agcagaacca ttatttattt ggctagaagt aaccagtcca ttgggggagg
    16561 agaagcctaa ggctagaggt gtgcagtgag ttggaattgc ctttaatgcc atcatacaaa
    16621 tgtggtcatg gtgacagcag gtccccaaat gcaatttgtt atgctagtct ggatcgtttt
    16681 agacgggagt atttaattcc cagattcctc acactttata aataacaact aaatcatgga
    16741 ataatgtctg aactcttttt gactttgagc tttcctgata atttcttaca agaaatagaa
    16801 gtaagccggg gaagaaattg ccactaattc tccatctttg tcagtcttgg acagatcact
    16861 atttgcagtg gaacatgtct gagtaccccg gagtgaaaaa tgttcgtttt ccagatggcc
    16921 agatttggaa accagacatt ctcctctata acaggtaagc ccgcctggca gatgggcaat
    16981 gatcttcact tgcatgcatg tggcgataaa ccatatttga gtattttaca ggaaagctgg
    17041 ctgtgttatc tttaatttgg aaccattgct ccttttgagg cttccaagtg ggcagatctc
    17101 tgggaagctg ctctgtccct ctgctggtat ttgcatcgaa gttgagatgg cgcaacgcaa
    17161 ttaatgataa cttcgtatag catacattat acgaagttat ggtctgagct cgccatcagt
    17221 tcactataga tgcataagat tgttccccat ttataggaat atgaagcact cttgaagcag
    17281 tgctttgcta tctctgaatg gccaccatca tagtacacag cagaaggtgc cagaccacat
    17341 gtgcattgga gaagacagtt tggacctagg ttcttatctg acttgttccc cattgtacac
    17401 cgtgtgtctg ttgcattttg accaagatcg gcagaacatg cttctcagag aaacatatgc
    17461 acaggctgaa gaagatctat aagagtcaag taatatggat gacagctatg tttttatatt
    17521 aggggcaaaa aaataaaggg atttatttct aattcaagaa tatgtgattt gcagacaaga
    17581 ggccaaaaag aggtaagttt taggcaggct taagttctaa aagtagcaaa ttccttgtca
    17641 ttagtaaggg agctacgaac atctctgtcc taaacagaat gaccagtgtg atgtcatgca
    17701 aaggggaagg tactggaaga tcattgtttc ttcgctcctg tctttggagc aggttttcct
    17761 gactcatagt actgtgactg tccaccactg ctcagtgacc cctcagagaa cgtccagggc
    17821 agctgatttg ttttcacttg ttacttccaa accctgggca gctcagtttc ttgtattcac
    17881 atttaattgc tttctgcctt tcccttggat ggctgaatgt gcgtctagga gaaaagcacc
    17941 tcctcgtttg ctaccactgt cagcttctta aaggaagtgc aggtaactga gctcctggga
    18001 tgtctgtcca aggtcagaat gactcgtcca tcactgaatg gttgaaacag ctgaactttg
    18061 ggtcatccat ttcctggaga ctgcactgat atcctgggtc tgctgtagaa cagcatcact
    18121 gaccactgtc ttaaacagca gacatttgtg tgctcatgat tttggaggat agatgagtga
    18181 gctgtaggtg tagccaggct tggcttctgt gtctttctac ctggattgta agtatcagcc
    18241 ttctcactat gtctgtacat tgtctcctgt gctatcttat atcctaatgt cttgccagaa
    18301 cacaagtcta gtgaccttct taactttaaa tactatgcgc gtgtgtgtgt gtgtgtgtgc
    18361 gtgtgtgtgc gcgcgcgcgt gtgtgtgtgt gtgtgtgtgt gtgtgttggg ctcttgctat
    18421 atagtaaaag atagccagga acccttgacc ttcttgcttc tactttttaa gtgctgggct
    18481 tccaagtatg caacaccata cctaactctt taattgactc tcttctgggc ctgtctctaa
    18541 atacagtcac attttcacgt accagggttt agaatttaaa tgtaaaaatg tgtggagaaa
    18601 caatttaggt ttgtaaggaa atttctgtag tgcatgaagc atacctagtg gcgtcctcag
    18661 caaaggggag gcattcagcc ctaacattca gtggtagttg tgtatttatt tgtgggagga
    18721 gctcgatgtc acctatcaat taaaactgtg tttcatagtt cctgatgagt tttatctgaa
    18781 agtatgaatg ttttttaatg tagtagaatt tcaataatga ggcaatgagg aatggtttta
    18841 tggctgtatg taagactgtt aactctaaag catgaagtgt ttgatgatct caaacttctg
    18901 gctaattgag agttactaat tagcctatat aattgatttc aaggatcatg gatagaacaa
    18961 cttccacctg gcatatggta ccaggctcta ggaatgtgtc ccctacagaa cacaaatcat
    19021 agtctagaac cctgcttgca gctacccttg agcagctttc ctgtcatctt ttccctgttg
    19081 atcacatcga cactgtccct tgtggagctc ataggggcca cctcaatgtt tccctcagtt
    19141 gttctaagca accagtgtta ttgtttgaat ctgaaataca ctgggagggg gcccttcagc
    19201 atatgctctc atcactttct gccctccgcc tttgattttc tggtaccctg taacatgaac
    19261 atctgtcttc taccagaagc tctggaaact atgatgttct gttcactctt ttgggcccaa
    19321 acagccatga agtaaacctt ctgagacaat gagccaaaag aaatagctcc ttccttaagt
    19381 tgtttcaggt cttttgtaca gtctatcaga aaagtaacta atgcattcta ttgttcagag
    19441 taactgtttt cacatacaag ttctgtgata aatcatctgc tagctgtctt ttatacccag
    19501 aatgtaaaat tcgaacttta tagaggcttt tatttaagcc agacatagac gatttgtgtc
    19561 attttttttc ttctattgct acaaaacaaa ctacctcaaa ctgagtggat taaaaatctc
    19621 agactcttac attattattc tttgtgatgc tttggcctga ccagaccaat ctgcttccat
    19681 gtctgctaca tatggtattg gaataccaca gttactgggg ctgaaggaca cacaccacta
    19741 gcctgggctg ctggcaggga tgacagccat ggctaaggat cacaccacct tggctttatt
    19801 cttcagtcct tttctccagt ttgatggcta ggggtatttt aagaagaagt aagtggtgtg
    19861 ggggctgctc tgcatctaag actgaacaca gaactcccat agcagccact cactccattc
    19921 tattgttcag agtagacagc agcccagtcc attcttgtcc ttttgtccac aaatcttcac
    19981 acttgtagag ttttaccctt tttcctcttt tggtgtgtta tgggtattcc tctgagttaa
    20041 gtctctgcct ccccctcctg catactcaag agagccagac tgatttcact gcccgtgact
    20101 gttctctaca aattgtcctt ttagcttcac atgcgtaatg tcgcatgtgt gtcactctca
    20161 atacatatac atacatacat acatatgtac aatacctaca tatatacata catacataca
    20221 tatactgtag gaaaggaggg caaaagataa atgaaataaa ggaaagaaat cacattagca
    20281 actcagaaaa caactgaacg agtcaaatat tcaacactag aggtagcaag cacaggcttg
    20341 tcttctgcaa gttcttactt taaggattct atactaggtg attttgagaa tggcaagctt
    20401 actttaagga ttctatacta ggtgattttg agaatggcaa gcttacttta aggattctat
    20461 actaggtgat tttgagaatg gcaagcttac tttaaggatt ctatactagg tgattttgag
    20521 aatggcaagc ttaatacttt ctgtctgttt caggcaatca gaatacagtg ctcagcccat
    20581 agatatactg tggtccatcc acagatggat aaaggtgttg ggcctgttcc caaagaaaga
    20641 ggttaagatg tcatcattct tagagataat gttagggcat ctggcgatgt cttgcaacca
    20701 tagagaattt cttgttccag tttggtgtct gagtccatgt gtataacact atggttttga
    20761 gatctaatgc attttttcca tgatttaaga ggcgggctca ctgtaaaaac agatatctct
    20821 gcgccatttc tagaatgggc tgataagttt tcttgataga tggggaacca gcgatgtggt
    20881 gccagccagc tagattgccg taaggagagg atgggcgtta ataagactct gcttgatgct
    20941 gattctagta gtttctcctt cactttattc tgcttcactt ggggccgtca actcaaggat
    21001 ttttctgaac acaatctatc tcaaaatgag ctagacaatt acagatacct tgtcacggtt
    21061 tccccttttt agatggcatt aaatgccttt ctggtgtcag tagtcatttc ccaaaaggga
    21121 attctgcatt gtgacataga ggtgggccct gtaactgcaa acaaaggtct tggctttcct
    21181 aggaatcttc tttctgtaat tgcctagtaa gagaaaattt tagtaataat gggaaatttt
    21241 taatgactat atttaaagat gtgttttata gcgctgattt tagcagcact tgtgatcaga
    21301 tatctctttc agagactgtg agacactatg tttgcacatc tgcaggaaaa tatctttctt
    21361 cccatgtccc tgggacccct tagttggtgg caaacatata gaaatgactg tagtgctaac
    21421 agattacat
//