LOCUS       208522#pL1L2_GTK_nEGFPO_T2A_CreERT_puro#L3L4_pD223_DTA_T_spec        21376 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GTK_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 208522
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000105420
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     misc_feature    4275..10009
                     /note="5 arm"
     misc_feature    10037..10061
                     /note="B1 Gateway"
     misc_feature    10074..10121
                     /note="Frt"
     misc_feature    10122..10622
                     /note="mouse En2 intron"
     primer          complement(10190..10211)
                     /note="LRPCR LAR3_EN2"
     primer          complement(10086..10109)
                     /note="FRT3"
     primer          14125..14143
                     /note="seq primer NF"
     misc_feature    14185..14192
                     /note="RE sites"
     misc_feature    15985..16018
                     /note="loxP"
     misc_feature    complement(14193..14224)
                     /note="Rox"
     misc_feature    complement(15945..15976)
                     /note="Rox"
     misc_feature    14225..14246
                     /note="polylinker"
     CDS             14805..15401
                     /note="puromycin N-acetyl transferase"
     misc_feature    14920..14925
                     /note="Mutated RsrII site"
     misc_feature    11159..11875
                     /note="EGFP"
     misc_feature    11069..11158
                     /note="3X NLS"
     misc_feature    13941..14179
                     /note="SV40 polyadenylation site"
     misc_feature    12974..13918
                     /note="ERT2"
     promoter        14257..14768
                     /note="PGK promoter"
     misc_feature    15512..15944
                     /note="PGK pA"
     primer          13999..14022
                     /note="LRPCR RAF5"
     misc_feature    11885..11938
                     /note="T2A"
     misc_feature    11935..11935
                     /note="A to G to kill RsRII site"
     misc_feature    11939..12964
                     /note="Cre"
     misc_feature    10897..10918
                     /note="seq primer FCHK2"
     misc_feature    10623..11059
                     /note="mouse En2 exon"
     misc_feature    11011..11079
                     /note="FCHK cre_ert_gtk"
     misc_feature    11011..11046
                     /note="FCHK QC prefix region"
     misc_feature    11047..11059
                     /note="FCHK QC critical region"
     misc_feature    11060..11079
                     /note="FCHK QC suffix region"
     misc_feature    16041..16061
                     /note="B2 Gateway"
     misc_feature    10010..16091
                     /note="Synthetic Cassette"
     exon            16425..16991
                     /db_xref="ENSEMBL:ENSMUSE00000666150"
                     /type="targeted"
                     /rank="2"
                     /note="target exon 1 ENSMUSE00000666150"
     misc_feature    16092..17145
                     /note="Critical Region"
     misc_feature    17169..17202
                     /note="Downstream LoxP"
     misc_feature    17146..17225
                     /note="synthetic loxP region"
     misc_feature    17226..21376
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggatgtgcc atgactctgt tccgaggctc tagctgcagg aatctttgtt
     4321 tttggcctca caatggcgtc cgggagccgg gagccaggtt agcggaagca ctgatggaca
     4381 gagggacgaa cagtgatagg gttcggtgga tcatactcag actggtccag tgcagcctgc
     4441 tctttacctt cagcctctgt ggctgcatca tcacatctct gtcccaagga ccacagcagt
     4501 gagcctgcat ccctagtttc tagtgctcag gctagcctcc tggtggggtt ccacctaggg
     4561 atcccttgtt ctcttcctag gaggtacagc acctgtcatc tgctatttat gggaggcacg
     4621 gatgttgagg ctttcgtggg atgctcttac catgacccgt gcacatggag aagccaaggc
     4681 acggtggggg tggcgggggg aagctggcgt ggaccttgca tggatgccac ttgggcagct
     4741 cccaccgcag gctgctctca cagtacttct gtgaggactg gatccaggga agggctttac
     4801 ccggagaact agcctgcaat ggatgagtgg tacctgaggt aggactgggg acctgtggtt
     4861 ctctctgggt accagaccta gacaagtggg gctgccaggg tcctccctag ggctctctct
     4921 ctgtgggaga gaagagagat gtggctagcc tgtggccttt gagtctcgag tctggactct
     4981 agactaaggt agcctggctc ctggctggtg gaggtagagt tatttagcag ggtaagctgg
     5041 tgccctggcc tccagctgca gaaggctggt tctcccttcc tgcccacatc ctgtccccag
     5101 gaacacaaca gcagccacat ggtccctccc acccatggtc gttgtgggag ggacttactg
     5161 gtcacctgct tgtatactcg gggcccagct tttaccaggt attagaattc gggtgctgtg
     5221 caattctaga agtatcagga agggaaaaga gacccagacc ccatagtcag tcaatgtggc
     5281 attaggcaac ctcaggagtg acccatcttg ttgactgatg tgttagggtg gggtatggta
     5341 ggttgggggg gggttcttcc tgcatctcca gttactgttg aaccactata tggcagttct
     5401 ttgggtaggg gaggtcccag tccatcctct tgtgattctt gcttggtgtc attcccccaa
     5461 atcgaaggac agatgttgaa tctcctcaat ctaaactttg tctctgattc cagtaagaac
     5521 tcctccgtgt tctctcttct ggccaccact gccttggtcc tcaactttac ctgcaggccc
     5581 aaagcttgtc atgtagagcg tgctctttat tttggcccct gtctttccca ctcaagactt
     5641 cgtgcttctt gctcgtcttt gctcttgtgt gccagcgagt ctaataacta tcgttcttgt
     5701 ttatggcgct tcctttttca gttatcaaaa taatcactac ataggctata cctccctctc
     5761 ctgtgggttt gatgtttagt atttttattt catggccttt tctttttgtt ttgttttgct
     5821 ttttgttttt aaggcagggt gtcactatgt taaccagact ggccaccacc tctcagagac
     5881 ccactaacca gtctctgcct ccagagtgct aggataaaag gtacacacca catctcttat
     5941 ggtctcagtt tcttgccttc tgaattctgg tttgtggctc atctgaagtg ggagctgagg
     6001 tcctagggtc agggcatggc ttgtaacaac ttccctgcca ccatccagga cccaagctat
     6061 ctgaatctct aaggtggcag ctgttattga agaattccca cagttcttgt cttttgagac
     6121 acctgcggga gaccccagga ctgaagggag caacaggggc agctgataga gttccccgtg
     6181 acacagacaa ctaggtagct ggtgaacatt gtccctgtga ggaagaggca gcctggtgtg
     6241 atggcacatg ccagtggaac tgtcacttag tgggtagaaa tgggaggatc aagagttcaa
     6301 gactatgcct ggatacatag caagcttgag gccagcctgg gctaacaggg acctgtcaca
     6361 aaacaaaatg gtaaggctgg gattgtagaa cccaggaaga aacccactgc agctgcaatg
     6421 ggcagtgtgt gtactgccct gtcccctctg cttggccgga gcaggagcag tgtgtgcagg
     6481 cctgcctgtg agctcgtaga cacatctagc agggtctgga gccaccacct gggcctgtgt
     6541 acacagagct tcgggtggct caggagagat gtgggttggg attaaatgat tttaattaaa
     6601 gtggctggtt caggatggga gcgaatgggg acgaagaggc ggcaatgcta aatctgaatg
     6661 ctgggcggcc gagcggctgt tccttcaagg ctctcgactc atgttccaca gtatgtgcct
     6721 ggaggtgacc tgggacagtg tctgtgctct accctgtccc caacagtgac ccatcataag
     6781 gaaatgtatt attaatattt aatttccccc cttggctagc aacagatgac tcaaggatta
     6841 gtccgataga gccaacagag agggatagga aattgctaga tcctggcgct acactggccc
     6901 cttctcctgg gccccaaacc ttcctgtgac cctggagcag cggttctcaa ccttcctaat
     6961 gctgtgaccc tttaatacag ttcctcatgt tggagtaacc tcccccctgc accccaacca
     7021 taaaatgatt tccgttgcta cttcataact aattttgttc ctgttatgaa tcacagtgta
     7081 aatattctgg gagatagagg ttttccaaaa gagacccaca ggttaaggac aacctgtgcc
     7141 gcgggagctt cacgaggagg aaggtctccc gcagcatccc ttggcattcc ctagcggcta
     7201 agccacgctt taacattccc ggtaaagccg agagtctttt cacctgtttg ggttctggct
     7261 tctcctctaa aagattgagt tcaccaggaa atcaaggaca tagctttcag ccactttcca
     7321 aaatgccatg agcatgcatg agttcctcag tggggcccag tgtagtcttg gtattcagat
     7381 cgacagccac ctggacaaca ccgggcttag tggttcagcc gcagctagca tctcttggcc
     7441 ctgagtgctg ctcagtccgg tcagtgaaac cttagtaggc ttggattgtc ccagtgggaa
     7501 cgttcatact acaaagaaaa gctacacgtc aaggtgccct ggccctgagc tatttagctg
     7561 tgtgcctaca gctagacagc ctcagccttc tgcctacagg gctcctccct cctgtctgtc
     7621 ctgagaatgt ctcagagctc tactgatctg ccggacttgg ggctggggaa ggcacaaagg
     7681 aaaagaagat ttgggtttgg cagctgccca ggtggcccca ggatcagagg agctttcccc
     7741 tgggatgcga agcaataact aagggcctgc aggaatgttg aaggaagggc agaggaaagg
     7801 gccctgggac aggaacctct agcgtgtctc agccaaagca aggcggcctg gcaggagggc
     7861 cagctgtgtt gagactggag atctgcagaa cagcttcggc gcagtcagga agagtactgg
     7921 ggtccagaaa tatccaacat gggcccagca cggaacacga gaagccttga gaactggcaa
     7981 tactcaggca ggtaccagag cggctgggag agcttcaggg aaaaaaaaaa aaaggcctgg
     8041 gttaggcccc tgcatagctg gtgtcctggt ggctatggag tcagtggtgt tacttggggg
     8101 aaaatactgg tggtgtgacc agaagcagca gcagggcttt aggctcaggg atggagccat
     8161 ctcacctcga cagaaatgtt tatgctccac tgtgttccac tggggacact gaggatggaa
     8221 aaatgttaag gcctgggtca gccttttagc atgggctggt ctgattgcag actgcttcag
     8281 tgccctctag tgaagattcc actagagaag ccctaaggga cataggtggt tatcctttgt
     8341 cccgaggaga cgccagtcag gagagtcagc attcaggggc tcactgggac tgatggggcc
     8401 tgaatggaca gtgagtccgc tgctgcttca aattctgaaa gctagaacag gagctatttt
     8461 agtcatggac ctgttgtggc cagatggacc cctgatgaat gctgctgtaa acacagaggg
     8521 acatggtatg tacctggagg tggcacccgt accagatctg gccattctgt ccagaatgtg
     8581 agttataaac agctcgttgg caagtagctt gtgcaaagct gggactcaca aggacttagc
     8641 cataggtgtc tctgcctgac cttaggagca atggcatcac ttagggacag taattccgtc
     8701 agttgctgca gactttgtct tctccttcca aaccttcagt cagcctcgta ggtgacaaag
     8761 gacaagctgc agctgtagcc acgctgactc tagactgaag cactggcaac atcgcagaag
     8821 caagcgtgcg gttcatttcc tttagctctc cattttacct ctcggccaat ttcaggtgga
     8881 gaggaccctt ctccccagtc ctcctgttcc actcatccat cctgtccttg aggagagaag
     8941 gaaagatgtg tctacatgca ggggtctgaa aattagcctc tagcttcttg ttcctagcag
     9001 gcacatctga ggtcccatgc aggtcaagta ctgcaaagga tggactggag ggcgggggta
     9061 ggggtgtcgt gggatccttt ggttcttgcc tggggaataa caggctattg ggagagtcct
     9121 gcttacgatg aagttttgca agctcatgga gcataccctg gggagtttgg gctcagctgg
     9181 ggccctgctg cggagagagc aggacttccc ctttccaggg gtctcagctc ttcctgtgag
     9241 ctgacactgt gactctaggg tagcctttcc aaatctacgg ataagcgctg aaaggagccc
     9301 tcggtatcat ctgggaccca aaggtgttac agtctgactg gagaaacgga ggcccgggaa
     9361 cgaaaagcat tcttgcccac ggtcctccaa tgagtcctta ctgagcacct gtttttctaa
     9421 gctgcattcc ctgcctcagt taggtaggca gagggacagg agaggcgagg tgtgggagga
     9481 aagcgggagg cggatccctc agagaaggga agcaatcgca gcgcctgccc tgcctggctg
     9541 tgcagcctgc tggcaggagc gctcttgctc tcggagcagt gggcttggct tggaccctgc
     9601 tcttgctgcc tacaagggta aggatgctta cttgctgaaa ctggaggata gataggacgg
     9661 gaaggctccc agatctctcc agaaaggagc tctggggagt gtctgaggtg accttagggg
     9721 caggggtgtc tctcaggtgg ggatggtagt atcctgaagg gatggagcaa aataaactgg
     9781 gcttggagga tggctcaatg cgagaaggca gggggcaggt gggatgggga aaggccagag
     9841 aggaaagaac attcctggaa ggagggcctt acgggactgg tagtagtgta gcggcccaga
     9901 ggactggcca ggcggcttca ggaagtttga aaaggtacat aagggatgtc aacataacgt
     9961 caacttgcct tgactttaac ttgctcccat agattaaaac aaagtaaaga aggcgcataa
    10021 cgataccacg atatcaacaa gtttgtacaa aaaagcaggc tggcgccgga accgaagttc
    10081 ctattccgaa gttcctattc tctagaaagt ataggaactt cgaacccttt cccacaccac
    10141 cctccacact tgccccaaac actgccaact atgtaggagg aaggggttgg gactaacaga
    10201 agaacccgtt gtggggaagc tgttgggagg gtcactttat gttcttgccc aaggtcagtt
    10261 gggtggcctg cttctgatga ggtggtccca aggtctgggg tagaaggtga gagggacagg
    10321 ccaccaaggt cagccccccc cccctatccc ataggagcca ggtccctctc ctggacagga
    10381 agactgaagg ggagatgcca gagactcagt gaagcctggg gtaccctatt ggagtccttc
    10441 aaggaaacaa acttggcctc accaggcctc agccttggct cctcctggga actctactgc
    10501 ccttgggatc cccttgtagt tgtgggttac ataggaaggg ggacgggatt ccccttgact
    10561 ggctagccta ctcttttctt cagtcttctc catctcctct cacctgtctc tcgacccttt
    10621 ccctaggata gacttggaaa aagataaggg gagaaaacaa atgcaaacga ggccagaaag
    10681 attttggctg ggcattcctt ccgctagctt ttattgggat cccctagttt gtgataggcc
    10741 ttttagctac atctgccaat ccatctcatt ttcacacaca cacacaccac tttccttctg
    10801 gtcagtgggc acatgtccag cctcaagttt atatcaccac ccccaatgcc caacacttgt
    10861 atggccttgg gcgggtcatc ccccccccca cccccagtat ctgcaacctc aagctagctt
    10921 gggtgcgttg gttgtggata agtagctaga ctccagcaac cagtaacctc tgccctttct
    10981 cctccatgac aaccaggtcc caggtcccga aaaccaaaga agaagaacgc agatcataac
    11041 tgactaggag gccaccatgg gcggaccggt gcatgtggac cccaagaaaa agcggaaggt
    11101 ggaccctaaa aagaaacgca aagtggatcc taagaaaaaa agaaaggtgc acgtgtccat
    11161 ggtgtccaag ggcgaggaac tgttcaccgg cgtggtgccc atcctggtgg aactggatgg
    11221 cgacgtgaac ggccacaagt tcagcgtgtc cggcgagggc gaaggcgacg ccacatacgg
    11281 aaagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgcctt ggcctaccct
    11341 cgtgaccaca ctgacctacg gcgtgcagtg cttcagcaga taccccgacc atatgaagca
    11401 gcacgacttc ttcaagagcg ccatgcccga gggctacgtg caggaaagaa ccatcttctt
    11461 taaggacgac ggcaactaca agaccagggc cgaagtgaag ttcgagggcg acaccctcgt
    11521 gaacagaatc gagctgaagg gcatcgactt caaagaggac ggcaacatcc tgggccacaa
    11581 gctggagtac aactacaaca gccacaacgt gtacatcatg gccgacaagc agaaaaacgg
    11641 catcaaagtg aacttcaaga tccggcacaa catcgaggac ggctccgtgc agctggccga
    11701 ccactaccag cagaacaccc ctatcggcga cggccctgtg ctgctgcctg acaaccacta
    11761 cctgagcacc cagagcgccc tgagcaagga ccccaacgag aagagggacc acatggtgct
    11821 gctggaattc gtgaccgccg ctggcatcac cctgggcatg gacgagctgt acaagcctgc
    11881 aggcgagggc agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccgggccgtc
    11941 caatttactg accgtacacc aaaatttgcc tgcattaccg gtcgatgcaa cgagtgatga
    12001 ggttcgcaag aacctgatgg acatgttcag ggatcgccag gcgttttctg agcatacctg
    12061 gaaaatgctt ctgtccgttt gccggtcgtg ggcggcatgg tgcaagttga ataaccggaa
    12121 atggtttccc gcagaacctg aagatgttcg cgattatctt ctatatcttc aggcgcgcgg
    12181 tctggcagta aaaactatcc agcaacattt gggccagcta aacatgcttc atcgtcggtc
    12241 tgggctgcca cgaccaagtg acagcaatgc tgtttcactg gttatgcggc ggatccgaaa
    12301 agaaaacgtt gatgccggtg aacgtgcaaa acaggctcta gcgttcgaac gcactgattt
    12361 cgaccaggtt cgttcactca tggaaaatag cgaccgctgc caggatataa ggaatctggc
    12421 atttctgggg attgcttata acaccctgtt acgtatagcc gaaattgcca ggatcagggt
    12481 taaagatatc tcacgtactg acggtgggag aatgttaatc catattggca gaacgaaaac
    12541 gctggttagc accgcaggtg tagagaaggc acttagcctg ggggtaacta aactggtcga
    12601 gcgatggatt tccgtctctg gtgtagctga tgatccgaat aactacctgt tttgccgggt
    12661 cagaaaaaat ggtgttgccg cgccatctgc caccagccag ctatcaactc gcgccctgga
    12721 agggattttt gaagcaactc atcgattgat ttacggcgct aaggatgact ctggtcagag
    12781 atacctggcc tggtctggac acagtgcccg tgtcggagcc gcgcgagata tggcccgcgc
    12841 tggagtttca ataccggaga tcatgcaagc tggtggctgg accaatgtaa atattgtcat
    12901 gaactatatc cgtaacctgg atagtgaaac aggggcaatg gtgcgcctgc tggaagatgg
    12961 cgatctcgag ccatctgctg gagacatgag agctgccaac ctttggccaa gcccgctcat
    13021 gatcaaacgc tctaagaaga acagcctggc cttgtccctg acggccgacc agatggtcag
    13081 tgccttgttg gatgctgagc cccccatact ctattccgag tatgatccta ccagaccctt
    13141 cagtgaagct tcgatgatgg gcttactgac caacctggca gacagggagc tggttcacat
    13201 gatcaactgg gcgaagaggg tgccaggctt tgtggatttg accctccatg atcaggtcca
    13261 ccttctagaa tgtgcctggc tagagatcct gatgattggt ctcgtctggc gctccatgga
    13321 gcacccagtg aagctactgt ttgctcctaa cttgctcttg gacaggaacc agggaaaatg
    13381 tgtagagggc atggtggaga tcttcgacat gctgctggct acatcatctc ggttccgcat
    13441 gatgaatctg cagggagagg agtttgtgtg cctcaaatct attattttgc ttaattctgg
    13501 agtgtacaca tttctgtcca gcaccctgaa gtctctggaa gagaaggacc atatccaccg
    13561 agtcctggac aagatcacag acactttgat ccacctgatg gccaaggcag gcctgaccct
    13621 gcagcagcag caccagcggc tggcccagct cctcctcatc ctctcccaca tcaggcacat
    13681 gagtaacaaa ggcatggagc atctgtacag catgaagtgc aagaacgtgg tgcccctcta
    13741 tgacctgctg ctggaggcgg cggacgccca ccgcctacat gcgcccacta gccgtggagg
    13801 ggcatccgtg gaggagacgg accaaagcca cttggccact gcgggctcta cttcatcgca
    13861 ttccttgcaa aagtattaca tcacggggga ggcagagggt ttccctgcca cagcttgatg
    13921 aagatctgag ctcttaatta atgatcataa tcagccatat cacatctgta gaggttttac
    13981 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
    14041 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
    14101 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
    14161 atgtatctta tcatgtctgg atccgtttaa actaacttta aataattggc attatttaaa
    14221 gttacatatg gcggccgccc taaggctcga cctcgaaatt ctaccgggta ggggaggcgc
    14281 ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca
    14341 caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt
    14401 tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg
    14461 ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg
    14521 tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat
    14581 agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg
    14641 ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcttccgga ggcccggcat
    14701 tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc
    14761 tttcgacctg catccatcta gatctcgagc agctgaagct taccatgacc gagtacaagc
    14821 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg
    14881 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc agatcgccac atcgagcggg
    14941 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
    15001 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
    15061 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
    15121 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
    15181 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
    15241 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
    15301 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
    15361 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgacgcccg ccccacgacc
    15421 cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc gatgatatca gatccccggg
    15481 atgcagaaat tgatgatcta ttaaacaata aagatgtcca ctaaaatgga agtttttcct
    15541 gtcatacttt gttaagaagg gtgagaacag agtacctaca ttttgaatgg aaggattgga
    15601 gctacggggg tgggggtggg gtgggattag ataaatgcnt gctctttact gaaggctctt
    15661 tactattgct ttatgataat gtttcatagt tggatatcat aatttaaaca agcaaaacca
    15721 aattaagggc cagctcattc ctcccactca tgatctatag atctatagat ctctcgtggg
    15781 atcattgttt ttctcttgat tcccactttg tggttctaag tactgtggtt tccaaatgtg
    15841 tcagtttcat agcctgaaga acgagatcag cagcctctgt tccacataca cttcattctc
    15901 agtattgttt tgccaagttc taattccatc agaagctggt cgagtaactt taaataattg
    15961 gcattattta aagttaggcg cgccataact tcgtatagca tacattatac gaagttatgt
    16021 cgagatattt aaatattaga cccagctttc ttgtacaaag tggttgatat ctctatagtc
    16081 gcagtaggcg gtaatcctag ctggaaggat gagtaactat agtgcctgga gtgagaacga
    16141 tgtatctttg gtaatgggga cgggtcagca ggaaatcctg gttctgtctg tagtctctcc
    16201 tttgtctgcc ctgaagagac tggggaagga atggtccctg agaagggttg ggtgaagatc
    16261 ttgatgagac ttctttggaa ccagtgagct ccttgggcct gtgtcctgac tgacagatcg
    16321 ggcaagatgg gagtcattct cagacagacg cctggcccac aacggacaca aaatctgaag
    16381 tggccagggt ggttcagctc ttcaccggga ctctctcttt ataggtatct cagaaccctg
    16441 aagctgagtg gagccaggcc cggaccccct gtagaagtca tggtttcggg agatgcagaa
    16501 ttatccgtga gaaagccttt gggaagctct ccctgggaac gctcgcggct cttgtgagga
    16561 aagggctgcg gggtttgagt gggtacacgg ctgggctgca gctatggacc gagagctggc
    16621 ccaggcccgc atccttgctg agcctgccca agtgtggctt ctcccgccat gggctcctcg
    16681 gtgtacatca tggtggagct ggccattgct gtgctggcca tcctgggtaa cgtgcttgtg
    16741 tgctgggccg tgtggatcaa cagcaacctg cagaacgtca ccaacttctt cgtggtatct
    16801 ctggcggcgg ctgacatcgc ggtgggcgtg ctcgccatcc cattcgccat caccatcagc
    16861 actggcttct gcgctgcctg ccatggctgc ctcttcttcg cctgctttgt cctggtcctc
    16921 acgcagagtt ccatcttcag cctcttggct attgccatcg acagatacat cgccatccga
    16981 attccactcc ggtgagccag gggttacatc tgtgcataag gctagatctg tgggcacaaa
    17041 gggcagggct ctgggactcc catctgaggc tttacagagc tgaaggtggc cgtgcctgtc
    17101 tggcttatcc ctatatgtcc agagtacttc ctaggtcaca tgtcggagat ggcgcaacgc
    17161 aattaatgat aacttcgtat agcatacatt atacgaagtt atggtctgag ctcgccatca
    17221 gttcaggaac cacagaacct caactaggaa cccgcctctg ccaccaccag cctttcccca
    17281 gtgtgtacct taggagggtg acccagagag gggaagtgac ttggccaaat gccacacagt
    17341 gagtcatttg gacttctatg caaattatat tcctcctagg tctgagagga ctctgcctgt
    17401 attaggggct ggctacacag aggggttttt ttggtggttt tttatttgtg tgtgtgtgtg
    17461 tgtgtgtgtg tgtgtgtgtg tgacttttct gtgaagtgac ttatgtgtga agaagttctt
    17521 cctggaggcc tcttctcagc caccctgtag ataacccagg cagtgcacta ctctctcagg
    17581 ggttcctcct ctgtgtcttc ctaccggagc taggtcctgt ggccagggtg gctgcagccc
    17641 ttgaggaaag attccataat gggggattgg gatgcatagt ctccttagcg agttttttct
    17701 gctcaagcag tgagggagca acttgactgt gggctgaggg catccagcaa taagaaggac
    17761 cagcctcagc gagctgtgaa aactcgggtt cagttgcaaa agagccttta gaggtcattt
    17821 ccttcctgcc gccttctgga tagaaaaagg aggctctgag aagggggcta gctctatcac
    17881 agccatcgga ggagtcggta ggagagaggg gaagaaacgt agcccaccac tactgtgacg
    17941 tcactttgaa gagtggtctg cctggagtgg ctcagtgccc agcaaggaat gtcacaacgc
    18001 tgttgttata atctgagcct aaggaagaag caagcgccca cgctgggctg ctcaagggaa
    18061 tggaggctat gccttgtgtc gccttaaaga cttggccaaa ggggccttca gccaggcatg
    18121 gtagcaagca cagcacatgt ttttgtcctt gaacttggca aaagctaaac cctgagaatg
    18181 gtgagttcca ggctagccag gagtctgcac taagacctct ggttcaagga aaagaaaaat
    18241 aatcaaagaa aataataaac aaagtggggg tggagggagg atttgggcca gctctaagaa
    18301 gtgacagagt taaaggtcgc aagtggggac agcttgtcct attgcttctg aagtagatca
    18361 aggtctacga tcctataggg gacaattaga acacagaggc ctacggggag tctgtgtgtg
    18421 agtcctcgtg ggtcaatagc atctacccca ggagacaggc tcagtctatg tagtagtggg
    18481 tcaccaaggg gaagcagtaa cttaaaattc cccatcttct aactccaagc agcaatgcat
    18541 ccggaaagat cagaataact gttctccatt taaaggtttc agcttgcttt tctctcctct
    18601 gcctctcctc gtctctgtct tctcagaatc aagagactga atccctgaaa taggaacgag
    18661 tcctgtggct atagaaagct agcggccctg tgccgggcct gttttctcac gggtaacaag
    18721 ggggcaagtt cctgtgctgt tcctcacaaa ggtctgcagg ttcactaagc gggtaggaat
    18781 gatgaggcag ggcagggtca cgggaaagga ttctgctctt tcatgagctg gaggcttgga
    18841 gccaacagcc tatccctata gtagttgcat cctccgacct cttgacctca gttccacttg
    18901 gagcttgagc catttgagct ctgaatgggc ccttgggaag cacaggctgg tctcggcaag
    18961 gagctgatag gtctgaagat gcccaccaag gatgctcagc ttgccttccg agcagtttta
    19021 cagctgccat ggccctgtta gtacaggatg tcctttgatc atcccagtcc agcatttcag
    19081 ccagagcaga tgtcatgacc cctgagggag accagagcca gaaaaggaca tctgtgactc
    19141 ccagtctgga tggactttag agcccttcac tcttcctgac cactgaactc ctgcactctc
    19201 ctgcctcttg aagtttcagg ctgatgggga gatggagaac accaggggac caggcatgag
    19261 ggggacctgg aggggctttc tagcctggac tcacttttct cccttcacag ctagggagtc
    19321 agtctaaggt cctattgaca aggtcacagg ctgagtcttt gacgaaggcg tagtcccagg
    19381 ctggcactgt gtaccgggaa ttctttcact gttgttgtaa agaattttca gcttcaggat
    19441 aatctgtccc ctccttcccg agcgtaggca ccctattaag ttaaggcaga accccgtgaa
    19501 tagtaaagaa aaccaggtca aaggcaaagg tgccacccta tgtctcaagg gatgaaaagc
    19561 ctggggtaag agaccaggct tcctttcctg gggagtcctt cacaccgtca cctctaagtc
    19621 tcacactggc ctgtggtgct cccatttgta agtaacaggc caaagtatgc aaaggcaatg
    19681 ggttggtaca agacctaaag tcctataaaa tacatttttt tttccgagtc cccaaaggac
    19741 aggagggtca ggcatatgca cgcttatgta gcatgctgga ggtcttgggc tatatcccca
    19801 gtaccacaaa atgtaaagaa agtaaaacaa aaaagctttc caagttagcc ataagcccct
    19861 actggactac agacatggct aacctatgtg aagccctgga ctgcattctt ccctccacag
    19921 tgatgctggt gaggatgcag tgtgccctgg aaggagtttc gccttatggg tgttgcagga
    19981 tctgaatggt cgtgagagtg taaaggggct gggggtgggg gacaggtctc aggcgagtcc
    20041 ctggtaaatg tcccaaagaa ggagtgagtg agcttgtgag tgtgacctct cctctgggcc
    20101 aaagagaggt agcctggctc ctgagtgcca ggtatggtgt gaggttgaat taagttggtc
    20161 tctgcctatg gaacagggtt gaatttctcc tttgggatta aagatgctct tcggttctta
    20221 ggtcattgtc tctgtaagat ggcttagaaa gtcctgcctt ctcctccgtg ttacaaatag
    20281 acatgaaaac tccacctgga gttgtgggtt tgttctagag ccatagggtg aggccccagt
    20341 agggttaatc agggaaaggc agtctcctca ggctggggga tatagggatg tagaggccac
    20401 ttgaaaacag gcactgctgg agactctgtc agccacatcc tgtatctctg aggctccctg
    20461 gaatggtcct atgccatgcc aggcagcctg agtatccttc tagctacact gagtgtattt
    20521 attcagaagg ccaggatgag actgagttag tcagaaatac aagccagcct gtgccctgga
    20581 gatacctcta gtttgtttgt gattccattt gagccccgat gctgtcctag acctcaggct
    20641 cctgttccat gaccttgatt tcctgatttt cctgcctctg tttccaaatg ctggaaataa
    20701 tgagcatgtg gtcctatttg gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt
    20761 gtgtgtgaga gagagagaga gagagagaga gagagagaga gagagaaaga gagagctcac
    20821 acaaatgcgc ttacataata cttgcatgtg ctcaaacatt gtcttcaagc ccctcagtac
    20881 tttgccaaca caggaaagta catctaacat attgggttct gtccccataa gggactttct
    20941 ttccagagtg ccgtggagga cttccccttg tgggttctct atctgtaaaa gtaaattgga
    21001 caaacctggg atttccatgg tgtagtctca gaagctgtca gacctgggct gttgtctcca
    21061 ataacaactt attaactcaa acttacttag ttggaacctt tctccaagat taccatcttc
    21121 ctaactacca ggaacagaaa gcctgttcca aaaacagttt gaaagttacc aagatgggag
    21181 accctacatc atccccagag gttcatcaat atcccaagaa gttcatttct ctgaccagca
    21241 actgaatagc tctgatctac cctgagccac agggtgactc cctggggttt ccagccacta
    21301 aattcagtat cgctccctta gaattcttag tttcatttca taaataagga aattgaggcc
    21361 cggagaggtg aagtga
//