LOCUS       195797#pL1L2_GT1_LF2A_nEGFPO_T2A_CreERT_puro#L3L4_pD223_DTA_T_spec        20939 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT1_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 195797
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000003643
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            4757..4809
                     /db_xref="ENSEMBL:ENSMUSE00000233054"
                     /rank="1"
                     /note="ENSMUSE00000233054"
     exon            7782..7993
                     /db_xref="ENSEMBL:ENSMUSE00000233047"
                     /rank="2"
                     /note="ENSMUSE00000233047"
     exon            8560..8714
                     /db_xref="ENSEMBL:ENSMUSE00000536429"
                     /rank="3"
                     /note="ENSMUSE00000536429"
     exon            9064..9196
                     /db_xref="ENSEMBL:ENSMUSE00000536428"
                     /rank="4"
                     /note="ENSMUSE00000536428"
     misc_feature    4275..9961
                     /note="5 arm"
     misc_feature    9989..10013
                     /note="B1 Gateway"
     misc_feature    10026..10073
                     /note="Frt"
     misc_feature    10074..10573
                     /note="mouse En2 intron"
     primer          complement(10142..10163)
                     /note="LRPCR LAR3_EN2"
     primer          complement(10038..10061)
                     /note="FRT3"
     primer          14239..14257
                     /note="seq primer NF"
     misc_feature    14299..14306
                     /note="RE sites"
     misc_feature    16099..16132
                     /note="loxP"
     misc_feature    complement(14307..14338)
                     /note="Rox"
     misc_feature    complement(16059..16090)
                     /note="Rox"
     misc_feature    14339..14360
                     /note="polylinker"
     CDS             14919..15515
                     /note="puromycin N-acetyl transferase"
     misc_feature    15034..15039
                     /note="Mutated RsrII site"
     intron          10574..10948
                     /note="mouse En2 intron"
     misc_feature    10946..10948
                     /note="Splice Acceptor"
     exon            10949..11002
                     /note="mouse En2 exon"
     primer          10849..10870
                     /note="seq primer FCHK2"
     misc_feature    11003..11125
                     /note="1Dleader"
     misc_feature    11126..11177
                     /note="F2A"
     misc_feature    11273..11989
                     /note="EGFP"
     misc_feature    11183..11272
                     /note="3X NLS"
     misc_feature    14055..14293
                     /note="SV40 polyadenylation site"
     misc_feature    13088..14032
                     /note="ERT2"
     promoter        14371..14882
                     /note="PGK promoter"
     misc_feature    15626..16058
                     /note="PGK pA"
     primer          14113..14136
                     /note="LRPCR RAF5"
     misc_feature    11999..12052
                     /note="T2A"
     misc_feature    12049..12049
                     /note="A to G to kill RsRII site"
     misc_feature    12053..13078
                     /note="Cre"
     misc_feature    10964..11003
                     /note="FCHK lf2a_lacz_gt1"
     misc_feature    10964..10979
                     /note="FCHK QC prefix region"
     misc_feature    10980..10997
                     /note="FCHK QC critical region"
     misc_feature    10998..11003
                     /note="FCHK QC suffix region"
     misc_feature    16155..16175
                     /note="B2 Gateway"
     misc_feature    9962..16205
                     /note="Synthetic Cassette"
     exon            16564..16735
                     /db_xref="ENSEMBL:ENSMUSE00000198481"
                     /type="targeted"
                     /rank="5"
                     /note="target exon 1 ENSMUSE00000198481"
     misc_feature    16206..16888
                     /note="Critical Region"
     misc_feature    16912..16945
                     /note="Downstream LoxP"
     misc_feature    16889..16968
                     /note="synthetic loxP region"
     exon            19238..19361
                     /db_xref="ENSEMBL:ENSMUSE00000233018"
                     /rank="6"
                     /note="ENSMUSE00000233018"
     exon            19906..20095
                     /db_xref="ENSEMBL:ENSMUSE00000233009"
                     /rank="7"
                     /note="ENSMUSE00000233009"
     misc_feature    16969..20939
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggatctaca gagtaaggcc ctatttcaaa aacacaaaca aaatggttct
     4321 cccagctgct aatgctcacc aggcaatgaa gcctggtgag cattagcaat gaaggcaatg
     4381 aaggagggtg ctggctacaa tcaaggctgt gggggactga gggcaggctg taacaggctt
     4441 gggggccagg gcttatacgt gcctgggact cccaaagtat tactgttcca tgttcccggc
     4501 gaagggccag ctgtcccccg ccagctagac tcagcactta gtttaggaac cagtgagcaa
     4561 gtcagccctt ggggcagccc atacaaggcc atggggctgg gcaagctgca cgcctgggtc
     4621 cggggtgggc acggtgcccg ggcaacgagc tgaaagctca tctactctca ggggcccctc
     4681 cctggggaca gcccctcctg gctagtcaca ccctgtaggc tcctctatat aacccagggg
     4741 cacaggggct gcccccgggt caccaccacc tccacagcac agacagacac tcaggagcca
     4801 gccagccagg tagggactga gagaaatcac tggggtggga gtggggcgtg ggagtccaag
     4861 ggtctgctca cccagtcatg ttatggttgt ggattttgca gcacaagttg tggggacaaa
     4921 tgtctgggac acctaggtct caatagccac caagtgtccc ctccttgcaa ggcagggtgg
     4981 gctggaactt agtttagcag agttaatggc ccacacaaag acagttgtct cagtgacacc
     5041 tgtcagtggc cctttaactt tgtaaccatg tggacctgtg ttgcagctct gtgaccttgt
     5101 gtctcactgt cctggtctgt ctctatgtct ctctgtctct ctgtctctat ctctctcttt
     5161 ctgtctctct ctctccctct ctctttcgag atgggtcagg ggggggtggt gttctctgca
     5221 tagccctggc tgtcctggaa ctcactctgt agaccagcct ggcctcgaac tcagaaatcc
     5281 acctgcctcc caagtgctgg gattaaaggc gtgtgccacc accgcccggc gggtctttct
     5341 tgtgtgagac ttgggggctc tcactcttac aggcccctgg ctttcctttg agtccttctg
     5401 tctggctgtc tctgggatct tgaaggcagg aaggactaca tgactcagtt tacctggaga
     5461 tcttagagaa tctgtgatga gtttggggat tccgaagctt tctgcttctg cgtcttgcct
     5521 cggtgtcctg tctcctgggg tgcccctgag ggagggggta gcagaggata cagaaccttc
     5581 tgaagggaga gatctgggct gggagcccgg ggtgtccttg aggcccagag cctggctgtg
     5641 tgtcctcctg gccaccccag cccacctgtc ccaatgctga cttagtgcaa ggcgagccag
     5701 caaggaggga ggacaggtgg cagtgggggg tgaggagcat ctaaaaatag ccacaaagta
     5761 gcagcttcaa gggctttggg tctctgtctg ccccacactc ttctctcagc ttggtccacc
     5821 ttccctctca ccttcctctg aggccccctt ccagccccga tggaggcctg atgtccccca
     5881 tggtcagtgc ttcagggatc tagtcaataa aattaataat gaaaaacaac agtaataaaa
     5941 tacacgtgac gtgactgggg cagcttaggg cttagttcaa atcccagtgt tcacaccctt
     6001 taaaagacaa gacaaaacaa aacagctggc tgtgggggag aacatcagaa tccccctggg
     6061 gaggtgggga caggggatct gtggggctcc atggccagcc agcctagctc caggcctgcg
     6121 agagacccta cctcaagata aaaataaaat aaaataaaat aaatatataa aataacaatc
     6181 ttgcagcacc tgaggtcacc actggaatgt gcacacctgt gcacatacat gagcctgcac
     6241 tacaaacaaa aatattaaca gtaactgtta gaatcccagc tgcaacttca tgccaggtgc
     6301 caggtccatg ctcatcagtc agggactgga actcagagat ctcctgggaa agcttcagtc
     6361 tcacagattc aaaagccaga gagatctagt cacagcctgg ggcccagagc agtgacttag
     6421 gagagccgtg ccttttaaag tggaccttgt agacagccag aggtggaggg actgggagaa
     6481 gtggctgaag cctccagact cattcccacg cccacatctg gactaatttg gatcagaatc
     6541 tcaggggagc ccttatggct tttctcaggt gtgcacatat aatctttacc agggtcctca
     6601 cacagagcct gtcagattgg ttttcaattt ctgtgacaaa caccatgacc aagacaacct
     6661 agaaaagaga aagcattaat ttggggctca gggttctgga gcggcaggga ggtgggcatg
     6721 gtgctggagc agaggctgga agctcacatc tttatcaaca accagaggca gtgagagcca
     6781 cttgggaatg gggtggcttt tcggaaatct caaagcccac aagcaatggc acacctcctc
     6841 caacaaggcc acacctccga atccttccca aacagttcca ccgactgggg accaaacatt
     6901 caaatatgtg agtctgaggc tcttctcatt caaatcacca cagacccaag aacaatcgaa
     6961 taaaatattt gtgttatgtg ccaggcactg gccgaggcgc ttttcttgtc ttttaatccc
     7021 tcccaagagg tcagcgatgc cacagtctcc atgttacaga tgagtgaaca ggaaagtcaa
     7081 acaggctcct cagagtcacg cggctgcttg taagttgcaa agccgaaatt cgaacccaga
     7141 ccatctgatc cagatccttt gctgctttta ttcatctttt tattttattt tattttattt
     7201 taattcctgg tggcagggtt tctgtagccc aggctaccct tgaattcact gcaatcctcc
     7261 tgcctcagtt tcagagtgtt ggaattacaa gcatggacca tcatgcccag ttcctttggg
     7321 ttgagataga gacctgtgta ggagcccaga ctcgggctgg tctccagctc tctacgtaga
     7381 tgaagatgac cttgaactgc tgggatttca ggcatgagca gccacaccca gatttgctga
     7441 gcgccaaact gttacccagg gtcctaagct tgctgggcaa gcactctgcc agcagaaccc
     7501 cagccccaga tcctgtattt ttgtagttgt ttttgtttat gtgactgtcc ttttctggct
     7561 ttagacaaaa ggttttgccc tccttttcca gctagagaga ctgagtcccc agcaggatca
     7621 cataggcagg atgtggccac atcaggcaac ttgggctcct gatgtttcct tgcaaggctg
     7681 aggttcacag ggggagaacc cccctttttc aagcccacgg tccgacggac tgcaagcccc
     7741 cagcaactga gttcttaagt ctgaacccgg tctgctcgca gggtcccaaa ggccgccacc
     7801 atgccgttcg gcaacaccca caacaagttc aagctgaact acaagcctca ggaggagtac
     7861 ccagacctca gcaagcacaa caatcacatg gccaaggtgc tgacccctga cctctacaat
     7921 aagcttcgcg ataaggagac accatccggc ttcactctgg acgatgtcat ccagactggg
     7981 gtggacaacc caggtgagtc tgcagcagga gagacagacc aaagggatgg agacagacac
     8041 ctgcctgttc cagggagact cagtttcctc ccttctctcc ctccctcttc ttgagttata
     8101 tctaggatat ctaggaactc ctacctgcac gctaggaagg gctctaccac tgagccacac
     8161 ccccagcccc tcactgggga ttctaggcag gggctctacc actgagccac gcccccagcc
     8221 cctcccaggg ggattctagg caggggctct accgctgagc cacgccccca gcccctcact
     8281 gggggattct aggcaggggc tctaccagtg aatcttgccc ccagctcctc aatgggggat
     8341 gctaggcagg ggctgtactt ctgagttgca ctccgggtcg tctgtgaaaa tggattgttt
     8401 gaggttctca gcacaggcta caatttgggg tctccaagga gctctaacat ctttggtccc
     8461 atttacctgg tccctggaat gacccttttc ccccacactg gaaacatcca tccatcacaa
     8521 acccaagctt cctatgctga cctcagcccc accccacagg tcaccccttc atcatgacgg
     8581 tgggctgtgt ggccggcgat gaggagtcct acacggtctt caaggacctg tttgatccca
     8641 tcatccagga ccgccatggc ggctacaaac ccacagacaa gcataagacc gacctcaacc
     8701 acgagaacct caaggtcact ggccaccagg gacaaggtgg ggacgagaga gggcggagat
     8761 gcagagtagg tggaggtatg ggaagacaga gaagacccct gagaccggat gggactccag
     8821 tgtcctgagg acaggggagg cagcgaggca gggtggggca caggaaggaa ctcagtaccg
     8881 gcatggggat ctgggaggat ctgggggaga tccagaagga agtcaggaaa ctccagagga
     8941 catgagggga cactcaggag gattggggag actctgaaag acctgcagtg agactcacgg
     9001 aagatttgaa gtgagaccgg gtatggccag gacttccttc cctgataccc caatgctccc
     9061 cagggtggag acgacctgga ccccaactat gtgctgagca gccgcgtgcg cactggccgc
     9121 agcatcaagg gttacactct gcctccgcac tgctcccgtg gagagcgccg tgcagtggag
     9181 aagctgtccg tggaaggtga ggtcccccac ggctgcaccc ggttcaggtt tcggttccat
     9241 ggttccatcg gagctggcct tggcaatgag aagaatggct gcattgcccg tgctggcggg
     9301 tgggggggtg ggagggtcac agcgcactga agcaacctgg gaagtcctgc actcaactgc
     9361 cccagggttt tcacacccat tgctgtctca gtcactcctt ggttcattaa ttcagtaatc
     9421 cccactctga gtggggagct gaaatttttc gggtgagggg gcatagcccg aatctgggtg
     9481 tggcagagga ctgacagagg gctgtttctg ttagttggcc tgggccaggc accctacact
     9541 tgtaatctca gtacttggaa ggctgagaca ggaggatcac cacgaattga agggccagcc
     9601 tttattcata gtgaggctct tcctcaaaaa caagaaagga aaacaaataa taacagatgt
     9661 tcataatgag tgcacatttc tctttctttt gttccactga gacagagact ccctatgtaa
     9721 cctaggctgg cctccaacta gtgatcctcc tgcctcagcc ttccaaatgc tgggattaca
     9781 aatatgtgcc ttagctggcc caaggtataa atctacagtg ggcagtggct aatcttttgg
     9841 aagtaggggg ttgggttgtt tgtttgtttg gtttttgttt tgtattttgt ttgtttgttc
     9901 tttatttgat tttttatatt ttgcagcatc tctctccata gccctaacta tcccagactc
     9961 aaaggcgcat aacgatacca cgatatcaac aagtttgtac aaaaaagcag gctggcgccg
    10021 gaaccgaagt tcctattccg aagttcctat tctctagaaa gtataggaac ttcgaaccct
    10081 ttcccacacc accctccaca cttgccccaa acactgccaa ctatgtagga ggaaggggtt
    10141 gggactaaca gaagaacccg ttgtggggaa gctgttggga gggtcacttt atgttcttgc
    10201 ccaaggtcag ttgggtggcc tgcttctgat gaggtggtcc caaggtctgg ggtagaaggt
    10261 gagagggaca ggccaccaag gtcagccccc cccccctatc ccataggagc caggtccctc
    10321 tcctggacag gaagactgaa ggggagatgc cagagactca gtgaagcctg gggtacccta
    10381 ttggagtcct tcaaggaaac aaacttggcc tcaccaggcc tcagccttgg ctcctcctgg
    10441 gaactctact gcccttggga tccccttgta gttgtgggtt acataggaag ggggacggga
    10501 ttccccttga ctggctagcc tactcttttc ttcagtcttc tccatctcct ctcacctgtc
    10561 tctcgaccct ttccctagga tagacttgga aaaagataag gggagaaaac aaatgcaaac
    10621 gaggccagaa agattttggc tgggcattcc ttccgctagc ttttattggg atcccctagt
    10681 ttgtgatagg ccttttagct acatctgcca atccatctca ttttcacaca cacacacacc
    10741 actttccttc tggtcagtgg gcacatgtcc agcctcaagt ttatatcacc acccccaatg
    10801 cccaacactt gtatggcctt gggcgggtca tccccccccc cacccccagt atctgcaacc
    10861 tcaagctagc ttgggtgcgt tggttgtgga taagtagcta gactccagca accagtaacc
    10921 tctgcccttt ctcctccatg acaaccaggc tcccaggtcc cgaaaaccaa agaagaagaa
    10981 cgcagatcgc agatcgcaga tctctagagt caccgagttg ctttaccgga tgaagagggc
    11041 cgaaacatac tgtccaaggc ccttgctggc aatccaccca actgaagcca gacacaaaca
    11101 gaaaattgtg gcaccggtga aacagacttt gaattttgac cttctcaagt tggcgggaga
    11161 cgtcgagtcc aaccccggac cggtgcatgt ggaccccaag aaaaagcgga aggtggaccc
    11221 taaaaagaaa cgcaaagtgg atcctaagaa aaaaagaaag gtgcacgtgt ccatggtgtc
    11281 caagggcgag gaactgttca ccggcgtggt gcccatcctg gtggaactgg atggcgacgt
    11341 gaacggccac aagttcagcg tgtccggcga gggcgaaggc gacgccacat acggaaagct
    11401 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccttggccta ccctcgtgac
    11461 cacactgacc tacggcgtgc agtgcttcag cagatacccc gaccatatga agcagcacga
    11521 cttcttcaag agcgccatgc ccgagggcta cgtgcaggaa agaaccatct tctttaagga
    11581 cgacggcaac tacaagacca gggccgaagt gaagttcgag ggcgacaccc tcgtgaacag
    11641 aatcgagctg aagggcatcg acttcaaaga ggacggcaac atcctgggcc acaagctgga
    11701 gtacaactac aacagccaca acgtgtacat catggccgac aagcagaaaa acggcatcaa
    11761 agtgaacttc aagatccggc acaacatcga ggacggctcc gtgcagctgg ccgaccacta
    11821 ccagcagaac acccctatcg gcgacggccc tgtgctgctg cctgacaacc actacctgag
    11881 cacccagagc gccctgagca aggaccccaa cgagaagagg gaccacatgg tgctgctgga
    11941 attcgtgacc gccgctggca tcaccctggg catggacgag ctgtacaagc ctgcaggcga
    12001 gggcagagga agtcttctaa catgcggtga cgtggaggag aatcccgggc cgtccaattt
    12061 actgaccgta caccaaaatt tgcctgcatt accggtcgat gcaacgagtg atgaggttcg
    12121 caagaacctg atggacatgt tcagggatcg ccaggcgttt tctgagcata cctggaaaat
    12181 gcttctgtcc gtttgccggt cgtgggcggc atggtgcaag ttgaataacc ggaaatggtt
    12241 tcccgcagaa cctgaagatg ttcgcgatta tcttctatat cttcaggcgc gcggtctggc
    12301 agtaaaaact atccagcaac atttgggcca gctaaacatg cttcatcgtc ggtctgggct
    12361 gccacgacca agtgacagca atgctgtttc actggttatg cggcggatcc gaaaagaaaa
    12421 cgttgatgcc ggtgaacgtg caaaacaggc tctagcgttc gaacgcactg atttcgacca
    12481 ggttcgttca ctcatggaaa atagcgaccg ctgccaggat ataaggaatc tggcatttct
    12541 ggggattgct tataacaccc tgttacgtat agccgaaatt gccaggatca gggttaaaga
    12601 tatctcacgt actgacggtg ggagaatgtt aatccatatt ggcagaacga aaacgctggt
    12661 tagcaccgca ggtgtagaga aggcacttag cctgggggta actaaactgg tcgagcgatg
    12721 gatttccgtc tctggtgtag ctgatgatcc gaataactac ctgttttgcc gggtcagaaa
    12781 aaatggtgtt gccgcgccat ctgccaccag ccagctatca actcgcgccc tggaagggat
    12841 ttttgaagca actcatcgat tgatttacgg cgctaaggat gactctggtc agagatacct
    12901 ggcctggtct ggacacagtg cccgtgtcgg agccgcgcga gatatggccc gcgctggagt
    12961 ttcaataccg gagatcatgc aagctggtgg ctggaccaat gtaaatattg tcatgaacta
    13021 tatccgtaac ctggatagtg aaacaggggc aatggtgcgc ctgctggaag atggcgatct
    13081 cgagccatct gctggagaca tgagagctgc caacctttgg ccaagcccgc tcatgatcaa
    13141 acgctctaag aagaacagcc tggccttgtc cctgacggcc gaccagatgg tcagtgcctt
    13201 gttggatgct gagcccccca tactctattc cgagtatgat cctaccagac ccttcagtga
    13261 agcttcgatg atgggcttac tgaccaacct ggcagacagg gagctggttc acatgatcaa
    13321 ctgggcgaag agggtgccag gctttgtgga tttgaccctc catgatcagg tccaccttct
    13381 agaatgtgcc tggctagaga tcctgatgat tggtctcgtc tggcgctcca tggagcaccc
    13441 agtgaagcta ctgtttgctc ctaacttgct cttggacagg aaccagggaa aatgtgtaga
    13501 gggcatggtg gagatcttcg acatgctgct ggctacatca tctcggttcc gcatgatgaa
    13561 tctgcaggga gaggagtttg tgtgcctcaa atctattatt ttgcttaatt ctggagtgta
    13621 cacatttctg tccagcaccc tgaagtctct ggaagagaag gaccatatcc accgagtcct
    13681 ggacaagatc acagacactt tgatccacct gatggccaag gcaggcctga ccctgcagca
    13741 gcagcaccag cggctggccc agctcctcct catcctctcc cacatcaggc acatgagtaa
    13801 caaaggcatg gagcatctgt acagcatgaa gtgcaagaac gtggtgcccc tctatgacct
    13861 gctgctggag gcggcggacg cccaccgcct acatgcgccc actagccgtg gaggggcatc
    13921 cgtggaggag acggaccaaa gccacttggc cactgcgggc tctacttcat cgcattcctt
    13981 gcaaaagtat tacatcacgg gggaggcaga gggtttccct gccacagctt gatgaagatc
    14041 tgagctctta attaatgatc ataatcagcc atatcacatc tgtagaggtt ttacttgctt
    14101 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
    14161 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
    14221 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
    14281 cttatcatgt ctggatccgt ttaaactaac tttaaataat tggcattatt taaagttaca
    14341 tatggcggcc gccctaaggc tcgacctcga aattctaccg ggtaggggag gcgcttttcc
    14401 caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg
    14461 gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg
    14521 gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc
    14581 agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga
    14641 tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc
    14701 tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca
    14761 ggggcgggct caggggcggg gcgggcgccc gaaggtcttc cggaggcccg gcattctgca
    14821 cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga
    14881 cctgcatcca tctagatctc gagcagctga agcttaccat gaccgagtac aagcccacgg
    14941 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg
    15001 ccgactaccc cgccacgcgc cacaccgtcg atccagatcg ccacatcgag cgggtcaccg
    15061 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg
    15121 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt
    15181 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac
    15241 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg
    15301 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag
    15361 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc
    15421 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac
    15481 cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac gacccgcagc
    15541 gcccgaccga aaggagcgca cgaccccatg catcgatgat atcagatccc cgggatgcag
    15601 aaattgatga tctattaaac aataaagatg tccactaaaa tggaagtttt tcctgtcata
    15661 ctttgttaag aagggtgaga acagagtacc tacattttga atggaaggat tggagctacg
    15721 ggggtggggg tggggtggga ttagataaat gcntgctctt tactgaaggc tctttactat
    15781 tgctttatga taatgtttca tagttggata tcataattta aacaagcaaa accaaattaa
    15841 gggccagctc attcctccca ctcatgatct atagatctat agatctctcg tgggatcatt
    15901 gtttttctct tgattcccac tttgtggttc taagtactgt ggtttccaaa tgtgtcagtt
    15961 tcatagcctg aagaacgaga tcagcagcct ctgttccaca tacacttcat tctcagtatt
    16021 gttttgccaa gttctaattc catcagaagc tggtcgagta actttaaata attggcatta
    16081 tttaaagtta ggcgcgccat aacttcgtat agcatacatt atacgaagtt atgtcgagat
    16141 atttaaatat tagacccagc tttcttgtac aaagtggttg atatctctat agtcgcagta
    16201 ggcggtggtg gtcactctta agccctccat aattatgtca cacatactga atttgaggcc
    16261 agcctaggct acctgatagg acccagtctc agaaacaaaa acgagacaag ccagatccgg
    16321 gactcacaca ctcttgcaga tggacttggt gggagggtct gctttatgtt gtacatgggc
    16381 acacagaagg aggtgtcagt gaacgctgag aacaggttac atgtggaatg taagtggttt
    16441 ggagacctct ttgatgtccg ggtgaggagt gttaggggca acaatagcgc gtgcagtctt
    16501 ccagtcttgg ccccccagcg gaagtgaggc aggctgccga ctgctctcct tgtggtccct
    16561 cagctctcaa cagcctgacg ggcgagttca agggcaagta ctaccctctg aagagcatga
    16621 cggagcagga acagcagcag ctcattgatg accacttcct gtttgacaag cccgtgtcac
    16681 ctctgctgct ggcctcaggc atggcccgag actggcccga tgcccgtggc atctggtgag
    16741 tccctccttt ctccagatcc tccggccctc tgacccttca atcgcaggag actttctatg
    16801 ggttaaaata gggtccctag tccggtccac cacagggtta gggccattca tgaaggcgga
    16861 tgtgggaggc ttgactgcac gcaccccaga gatggcgcaa cgcaattaat gataacttcg
    16921 tatagcatac attatacgaa gttatggtct gagctcgcca tcagttcaag taggttctaa
    16981 ctcttggaca tcgcaggcac tactgggtgc ctcggaagag ctgagaacag agttggggcc
    17041 caagggctgg ctctagcccg gggccaaagg aaaaagggca agaggagagg gctctgcttg
    17101 ggtcccatgg ggaggagagt cctagcttgc tcaaagggca ggttggcttg acttggtgga
    17161 aatcgtgtct ggaagcaccc gctggagact aggcatggct cttcagagga agacttgctc
    17221 cattgctcca gcacggcgat cccgatgaga agagacagtc catggcttta aagtgtttat
    17281 tgtagaaagg cagagagagg gagagagtag agaagtagag gttggccatg gcaggtggag
    17341 agagggagga agagaatgca gagaggcagg gagcaagaga gagagagaga agcaagctgg
    17401 aagcagaagt aagagaggga ggaggggtca agcagcccct tttacagtgg gccaggcata
    17461 cctggctgtt gccaggtaac tagaagtgga gtccagacag aataccaggg gcttggggtg
    17521 ttacccgacg tgactgatga tgccacaaaa ttatggagct ggggccttgt gtcaggagcc
    17581 tagtgtctgg gagcatggaa aacttccttc cgtctcttgc agagttatct gctgggtctc
    17641 aggggtttaa acctcgttca accagaaaac aggctgcctt ttacggtccc actgccttct
    17701 aaccctgcct atccctgggc aggtgtcata gtttagaaaa cttgaatcaa ggaagtgtaa
    17761 tgaaattatc ttagatctgt ggcataggcc tggaaggagg tcctgagctc agggccaacc
    17821 agactacagt gaatttgaga ccagcttagc ctcaaaacaa gaactgaaca gagagctgga
    17881 gatggagttg tggctcagtg gtagagcccc tgcctagaat cccccagtga gggtctgggg
    17941 gtgtggctca gtgggagagc ccctgcctag aatcacccag tgagggtctg ggggtgtggc
    18001 tcagtggtag agtccctgcc tagaatcccc cagagagggc tgggggcgtg gctcagtggt
    18061 agtgcccttg cctagaatcc cttagtgagg gactgggggc gtggctcagt ggtagtgccc
    18121 ttgcctagaa tcccccagtg aggggctggg ggcgtggctc agtggtagag cccctgccta
    18181 gaatccccca gagagggctg ggggcgtggc tcagatggag agaatctgcc tggcatgcat
    18241 agtgcccaga tttaatcctt attatggcaa ataaaataaa ataaaacatt gatactggaa
    18301 ctttatactc tctggattca aaccccagct gttactaggt atatgaccat acattcctgc
    18361 agtatcagct actccggact cctaaggcag gagggtcact tgatgtcagg aaccactctg
    18421 gccaacaaag accatctttc taaaaatcca ttgtacaagg ccaggtggta gtggcacaca
    18481 catttaatct cagcactcag gaggcagaga cagatgggtc tctgagattg aggccagcct
    18541 gatctacaaa gtgagtttca ggacagccag ggctacacag agaaaccctg tcttaaaaca
    18601 acaacaacaa caacaacaac aaaatgcatt gtacaacaaa atacacatat gaaaatacaa
    18661 caacaacaac agcagcagca gcagtaataa taaatcatcc aggcttggtg gtgcacgcct
    18721 gcaatcatca tactgcagag cctgaggcag gggaattacc agttcaatgg cagccaagac
    18781 ccctcccttt ctgaacaaaa aataattaaa attgttacat taatgataat tttaaaaaac
    18841 tgaagattgc tgaactctca gaaccctggg atgcaaatcc gaaaggagaa tcttgggatc
    18901 caaggggata taacattggg tgtaggacct gtggtcacag gaggtataca acgcccctga
    18961 acccgaagct gacctcgaac tcaaggtctt tacacctcag tcttctgggt gcctgcaggg
    19021 gaggggtcaa gtaagactga aggttttcag ggacttgggc ccatcaaagt ccctgttctc
    19081 tgctccgaag tttcgtggag ttgtgactgg gaagccagtg tgcccgtgtc tgtcccctgg
    19141 agcccaaagg gagcagctat gaacaaagtc atgcgtttac tttcttgggc cattggggct
    19201 cctgctgact tcttggtctt gggtcacccc ttcttaggca caacgacaac aaaagcttcc
    19261 ttgtgtgggt gaacgaggag gaccacctcc gcgtgatctc catggagaag ggaggcaata
    19321 tgaaggaggt tttccgccgc ttctgcgtgg gcctgcagaa ggtgggtggc tgcgtcctga
    19381 ttgcaacccc atgtaaagcc aagctccttc ctaaacccag ccctgccctt ccaaaccctc
    19441 cacgcccagt tcctaaaact cctccccttc cacactggcc aagcccatgc tggcttttca
    19501 tcacccactt tccagtctct gcctcttaca cacattcaca cacatgcaca tatacatgcc
    19561 ttttctgatt tcactcccta agaaggttcc gaccattccc caccctcgcc cgttttccca
    19621 tcctcacttt ccagccccag acccagccct accattcaga actcacctcc cccatttacc
    19681 aagccctgtt cccatcccca gggtctgaaa ccagccccca tctttgccca aggcctttct
    19741 gggcagaatt cctcctggtt gtttctccac tctcacgcgc ctacccccac ctatgtccta
    19801 acacctggtc cctcacacac acacacacac acacacacac acacacacac acacacccca
    19861 gactgccttt ggagtccaat tcctggtgtc ctctcccttt ctcagattga ggagatcttc
    19921 aagaaggctg gtcacccctt catgtggaac gagcacctgg gctacgtgct cacctgcccc
    19981 tccaacctgg gcaccgggct gcgcggaggc gtgcacgtga agctggcgaa cctgagcaag
    20041 caccccaagt ttgaggagat tctcactcgc cttcgtctgc agaagcgcgg cacaggtgcg
    20101 gcccgccccg ccccgccccg ccccgccccg ccccgcggcg tctggggagg gatctaccga
    20161 ggaggggtgt ccctgcaagt tccgggatta agagtatttg atgtgtattt tacgtacatc
    20221 cccgatcctc tattggcggc tcagattctc tacctgtaaa tggaatgggg tcagtttgac
    20281 cgcaggctga caatcttggt ttttttgttt gtttgttttg tttttcgaga cagagtttct
    20341 ctgagtagcc ctggctttcc tggaattcac tctgtagacc aggatgccct cgaactcaca
    20401 gaaatcctcc tgcctctgtc tcccgagtgc tgggatttaa aatgtgcatc accattgccc
    20461 ggtatttttt tttttttttt tttttttttt tttttgggtt tttgagacag ggtttctctg
    20521 tatagccctg gctgtcctgg aactcactct gtagaccagg ctggcctcaa actcagagat
    20581 ccacctgcct ctgcctccca agtgctggga ttaaaggcgt gcgccaccac gcccggctcc
    20641 ggtatatttt taaaattaaa tttgtctgtg tgagagagag cactcccact tcacgacact
    20701 tgcatggatg tcagaggaca actttctaaa gtcagttcct gccttccacc acgtgagccc
    20761 tgaaaattga acccggatgt caggtttagc aaaaagcatc tttactcact cagctatttc
    20821 gctggcctgt gctataattt cttgttgagc tgagacctca ccctaacatc caggcataca
    20881 gggcctaata tcaataactg gtgacttgtg aacagcaact ttccgagacg tttgcactt
//