LOCUS       46369#pL1L2_GT1_LF2A_nEGFPO_T2A_CreERT_puro#L3L4_pD223_DTA_T_spec        21075 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT1_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 46369
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000029975
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            8349..8462
                     /db_xref="ENSEMBL:ENSMUSE00000177972"
                     /rank="2"
                     /note="ENSMUSE00000177972"
     misc_feature    4275..8963
                     /note="5 arm"
     misc_feature    8991..9015
                     /note="B1 Gateway"
     misc_feature    9028..9075
                     /note="Frt"
     misc_feature    9076..9575
                     /note="mouse En2 intron"
     primer          complement(9144..9165)
                     /note="LRPCR LAR3_EN2"
     primer          complement(9040..9063)
                     /note="FRT3"
     primer          13241..13259
                     /note="seq primer NF"
     misc_feature    13301..13308
                     /note="RE sites"
     misc_feature    15101..15134
                     /note="loxP"
     misc_feature    complement(13309..13340)
                     /note="Rox"
     misc_feature    complement(15061..15092)
                     /note="Rox"
     misc_feature    13341..13362
                     /note="polylinker"
     CDS             13921..14517
                     /note="puromycin N-acetyl transferase"
     misc_feature    14036..14041
                     /note="Mutated RsrII site"
     intron          9576..9950
                     /note="mouse En2 intron"
     misc_feature    9948..9950
                     /note="Splice Acceptor"
     exon            9951..10004
                     /note="mouse En2 exon"
     primer          9851..9872
                     /note="seq primer FCHK2"
     misc_feature    10005..10127
                     /note="1Dleader"
     misc_feature    10128..10179
                     /note="F2A"
     misc_feature    10275..10991
                     /note="EGFP"
     misc_feature    10185..10274
                     /note="3X NLS"
     misc_feature    13057..13295
                     /note="SV40 polyadenylation site"
     misc_feature    12090..13034
                     /note="ERT2"
     promoter        13373..13884
                     /note="PGK promoter"
     misc_feature    14628..15060
                     /note="PGK pA"
     primer          13115..13138
                     /note="LRPCR RAF5"
     misc_feature    11001..11054
                     /note="T2A"
     misc_feature    11051..11051
                     /note="A to G to kill RsRII site"
     misc_feature    11055..12080
                     /note="Cre"
     misc_feature    9966..10005
                     /note="FCHK lf2a_lacz_gt1"
     misc_feature    9966..9981
                     /note="FCHK QC prefix region"
     misc_feature    9982..9999
                     /note="FCHK QC critical region"
     misc_feature    10000..10005
                     /note="FCHK QC suffix region"
     misc_feature    15157..15177
                     /note="B2 Gateway"
     misc_feature    8964..15207
                     /note="Synthetic Cassette"
     exon            15621..15805
                     /db_xref="ENSEMBL:ENSMUSE00000177970"
                     /type="targeted"
                     /rank="3"
                     /note="target exon 1 ENSMUSE00000177970"
     misc_feature    15208..15989
                     /note="Critical Region"
     misc_feature    16013..16046
                     /note="Downstream LoxP"
     misc_feature    15990..16069
                     /note="synthetic loxP region"
     exon            17627..17931
                     /db_xref="ENSEMBL:ENSMUSE00000233907"
                     /rank="4"
                     /note="ENSMUSE00000233907"
     misc_feature    16070..21075
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggagcagag tattccttca gaataccaaa ataggttaag attaataagc
     4321 ctaagtttaa atttagattg atcatagttt aatagcataa ctgatcatag ttgcatagta
     4381 taagttgcaa ctggatataa ttattatgtt tttctctcca gttcttaagt ttttcttcca
     4441 agttgtgaaa tagataaata cctgcgattc cagcaaccag gaggtggcaa taggggaatt
     4501 tccaggaatt caagcctagt ctggactgtg tagtgagttc cagaccaggc ggggctacgc
     4561 tcccacaggg ttagcaatta ggggaacata aatgaaagag attgtgagat tgtatcttgt
     4621 accaggcaga ctggctaaga tcaagaaaac aactgacaac gaggttgcca cggatgtggg
     4681 gaagggaaat cctcgctcgc tcactgttgg agggattgca aacagatccg gttaccgtgg
     4741 aaaccagtgt gggggattct taaaaacatt gaaagcaaat gtatcatatg acccagctct
     4801 actatgtctt ggtacagacc caaaggactc agcaccctcc tccaaagatg atcggctcag
     4861 ccattttcat tgctacctca ctcatggcca agaaatggaa actgcctaaa cgtcctctag
     4921 ttgatgaatg gacaacggag acataacgga ttcctattca gttttaaagg aaaaaaaatg
     4981 aaataataaa gtttgacagg aaatagacgg aactagaatc gattatctta tggaatgaga
     5041 tggcaggaat ccagaatctg tgcatctctt agctgatgaa gctaagttct gggtctttag
     5101 atagataacc tggagtgact gcaacactag gaaagtacaa agggaccatg gtgggagcat
     5161 aggggagggc aggagcacta gaaaggggaa cagcagcaca cagtttctat gaagaggaaa
     5221 tgggcgaaat ggaagggaag gaaggaggag gggcagcaca gagggagtgg aaagggtgag
     5281 ggatgtttgg gaaagctata ggaattattc tatggctaca taaaatcaca tctagtctct
     5341 atctatctat ctatctatct atctatctat ctatccatct gtctacatat gctgtgtgta
     5401 tgtatgtatg tatgagtgta tgtatgtatg tatgagtgca tgcatataat gtaagaccaa
     5461 tgacgaccca agtgagactc tgagatgcag atatgtgcaa aatcaagagg tttattttct
     5521 gcatcggagt cgaccttcaa ttagtccctc aaggcaagtt actaaggagg tgaccctgaa
     5581 tggcttttac aagtggtttt tatacttttt agggacacag gttgcatcag caaggtcaca
     5641 gttcaaactt attgactaag catattgact tttaaatcta ttggctagca agcaggacat
     5701 gcatttgaac tctacctggg actttccagg ggaccaggtg atggtcagta cattctgaga
     5761 attttttact caagagtgtt cccgtgggtg gacctagcct tgaccccagg tgagaggggg
     5821 aagctgtaac tgtaacgagg gtgtgggact tggagaaagt ccttagggtc cctcagtatc
     5881 tagctatctc acttactgtt atgccaattg ggtgacaata ttcttcctta aaccacagaa
     5941 taacaaaaac tgaagtttat atatgtctat atatccatct gtctgcatat gttatatatg
     6001 tatatagtat atatatatat tttatatata gatatagtac agggggtgga ataagaggta
     6061 gtcagaaaat caatgtgctt aaccataaat ttcataatca agctaatagc aaacccagtg
     6121 tttgagggct aggggtgtga ctcagttgac agagtgcttg cctagctccg ttcactgcat
     6181 aaatcaggta aaagggacgc agctgcctgt ttaatctcaa ccttccctgg tgaacgccag
     6241 agtatcagaa gttcagagac tacactattt tgccagtttc aggtcagcaa gaacaaatag
     6301 acaaacaaaa caaaacaccc cacaacctac tgtttattta agaggcaaga tgtctgcctt
     6361 acaactgaat ccttttaaaa caaacaacag cgttgacaca cctggtcatt ttactgttgt
     6421 gttctgactg cactgtattt ttctaaggat agttatctaa aatgaacaac cagcctggag
     6481 gttggctcta gcagaactgc agcacgaagg aagctggagc tgagggtctc ccggtccagt
     6541 ccgcccgggc tacattgtaa tgctgggtct cgaaaacata atttctatta tattcagtgg
     6601 ccttttcagg aaatgtatat aataaagcca gatgtgattc tagtcctcac aggtgacggg
     6661 aggaggacac taagtggaaa atgttctctg atactgtgtt gttccagcga atgttttgta
     6721 atgcatgctt ttaaatcatt acataagatg aacaagcaca aggcaacctt acagaaaaca
     6781 gcatcaatac tgcacattaa caagacagaa tcctctaatg cttgcatgta catgagacca
     6841 ggggagcaca ctgtaaggct aaggatttcc tctattggat tgaacagtga aaaagagttg
     6901 aagaaacagg ctcgggagat ggctcagtgg ttaagagcac agactgccct tccagaggtc
     6961 ctgagttcaa ttcccagcca ctacatggtg gcttataact gcccccctcc cccatgagtt
     7021 ctgatgccct cttccacttg ggaggcagag gcaagcagat ttctgagttt gaggccagcc
     7081 tggtctacag agtgagttcc aggacagcca gggctctggc tctctctgta gaccaggctg
     7141 accttaaact cagaaattcg cctgcctctg cccccaagtg ctgagattaa aggcatgtac
     7201 caccatgccc agcttaaata agtcttttaa actttttttt tttttaatca ctggatcttc
     7261 tgacaaaatc agaaatctta ataagaaaaa attcttgaag agctgtgttg ctctccagag
     7321 atttccagat agctcagctc tgactaaaga aagtttctaa agaagaacag ttattgcttt
     7381 ctgctagctc atctctggca gaccacagcc caggttttta tttacataac aatctgtcat
     7441 ttatccaggt ttcctttcaa ttatcttgag aaccagcagc cctggagtcc agtcccttgc
     7501 tctccagaga tagaaagcat acattttttt tttcttaacc ttgcaaaaga atgcagaaat
     7561 ctgtctgctt ttgttaataa acttgttggg tgggaccctg ccctgaaatt accatttcca
     7621 ccaaacctgg cctaaccttg ctgtgtccta ggctgaacta gaactcagga atctgcctgc
     7681 ctttgtattt ttctaacaag ctggctcatg gtgtacctgc ttttgttcct ttagattcat
     7741 gaagcctctc ataattcata ttgcatcttt atattttata tagttgagaa attatatatt
     7801 ttgaacttat gtatctagaa ctctctccca tagccttaag ggcagtcctg aagtttccag
     7861 gatttaccac tttgctgaga cattaatgtt atggagtctt taaatgttaa ctgggagaat
     7921 attccttggt ggaatgtgaa aatacgtgca tttttataca aacaagcctt aagcccacag
     7981 cagccatcaa cccttgtgga ggctcacaca gggccctgtc ccaggggcag gaaccctgtc
     8041 tgtcactctg tccctaccag ggccacacct ggcttggcaa gatcccattg cagatggttg
     8101 tgagccacca tgtgactgct gggatttgaa ctcagaacct ctggaagagc agtcattgct
     8161 cttaaccact gagccatctc tctgagcaat aatgttaaac ttactggttg atggtgctgg
     8221 ggatacagct cagtcgtaga gcagtactta gcacgcccaa agtcagcacc atcatactaa
     8281 aacagctggc ttgggttatg actggtaagc taagattaca ctgttattat tttttttttc
     8341 atgtgcagct tgcagaagag ctatggatta ctacagaaaa tatgcagctg tcattctggt
     8401 catgctgtcc atgttcctgc atattcttca ttctcttcct gatggagact ttattattca
     8461 gggtatgtga ctaagctcag gcttgccatg aaaagggaaa caagtttcta gatgcacatc
     8521 aataatgatt agttcctttc taccgtcttt cacatgtgat aaatagtcac ttctggatac
     8581 acatcttaaa cacacgtagc ccgtgagcac agaattgaag taggttcaga aaaataggca
     8641 ctgaaaatgc tatatcccac gttcgcttta atacattcta agcatgttca tctgaagtcc
     8701 actcgtatag ttccacatcg aggttgacaa ggagaaaagc catccctact cgtctcagtt
     8761 gtcttttcac atcgcttcca gatttcagaa aaatttatca ttgctatcac agataaattt
     8821 tcatatcagg ataaccagca aggaaaataa aaatccgttt catgcactaa atcagctttc
     8881 tttcactgtt tgaaattgtg aacgaacagt tatttgttaa ggttttcctt ttgtaaacgg
     8941 taatgtttgt atctcaggtt ctaaaggcgc ataacgatac cacgatatca acaagtttgt
     9001 acaaaaaagc aggctggcgc cggaaccgaa gttcctattc cgaagttcct attctctaga
     9061 aagtatagga acttcgaacc ctttcccaca ccaccctcca cacttgcccc aaacactgcc
     9121 aactatgtag gaggaagggg ttgggactaa cagaagaacc cgttgtgggg aagctgttgg
     9181 gagggtcact ttatgttctt gcccaaggtc agttgggtgg cctgcttctg atgaggtggt
     9241 cccaaggtct ggggtagaag gtgagaggga caggccacca aggtcagccc ccccccccta
     9301 tcccatagga gccaggtccc tctcctggac aggaagactg aaggggagat gccagagact
     9361 cagtgaagcc tggggtaccc tattggagtc cttcaaggaa acaaacttgg cctcaccagg
     9421 cctcagcctt ggctcctcct gggaactcta ctgcccttgg gatccccttg tagttgtggg
     9481 ttacatagga agggggacgg gattcccctt gactggctag cctactcttt tcttcagtct
     9541 tctccatctc ctctcacctg tctctcgacc ctttccctag gatagacttg gaaaaagata
     9601 aggggagaaa acaaatgcaa acgaggccag aaagattttg gctgggcatt ccttccgcta
     9661 gcttttattg ggatccccta gtttgtgata ggccttttag ctacatctgc caatccatct
     9721 cattttcaca cacacacaca ccactttcct tctggtcagt gggcacatgt ccagcctcaa
     9781 gtttatatca ccacccccaa tgcccaacac ttgtatggcc ttgggcgggt catccccccc
     9841 cccaccccca gtatctgcaa cctcaagcta gcttgggtgc gttggttgtg gataagtagc
     9901 tagactccag caaccagtaa cctctgccct ttctcctcca tgacaaccag gctcccaggt
     9961 cccgaaaacc aaagaagaag aacgcagatc gcagatcgca gatctctaga gtcaccgagt
    10021 tgctttaccg gatgaagagg gccgaaacat actgtccaag gcccttgctg gcaatccacc
    10081 caactgaagc cagacacaaa cagaaaattg tggcaccggt gaaacagact ttgaattttg
    10141 accttctcaa gttggcggga gacgtcgagt ccaaccccgg accggtgcat gtggacccca
    10201 agaaaaagcg gaaggtggac cctaaaaaga aacgcaaagt ggatcctaag aaaaaaagaa
    10261 aggtgcacgt gtccatggtg tccaagggcg aggaactgtt caccggcgtg gtgcccatcc
    10321 tggtggaact ggatggcgac gtgaacggcc acaagttcag cgtgtccggc gagggcgaag
    10381 gcgacgccac atacggaaag ctgaccctga agttcatctg caccaccggc aagctgcccg
    10441 tgccttggcc taccctcgtg accacactga cctacggcgt gcagtgcttc agcagatacc
    10501 ccgaccatat gaagcagcac gacttcttca agagcgccat gcccgagggc tacgtgcagg
    10561 aaagaaccat cttctttaag gacgacggca actacaagac cagggccgaa gtgaagttcg
    10621 agggcgacac cctcgtgaac agaatcgagc tgaagggcat cgacttcaaa gaggacggca
    10681 acatcctggg ccacaagctg gagtacaact acaacagcca caacgtgtac atcatggccg
    10741 acaagcagaa aaacggcatc aaagtgaact tcaagatccg gcacaacatc gaggacggct
    10801 ccgtgcagct ggccgaccac taccagcaga acacccctat cggcgacggc cctgtgctgc
    10861 tgcctgacaa ccactacctg agcacccaga gcgccctgag caaggacccc aacgagaaga
    10921 gggaccacat ggtgctgctg gaattcgtga ccgccgctgg catcaccctg ggcatggacg
    10981 agctgtacaa gcctgcaggc gagggcagag gaagtcttct aacatgcggt gacgtggagg
    11041 agaatcccgg gccgtccaat ttactgaccg tacaccaaaa tttgcctgca ttaccggtcg
    11101 atgcaacgag tgatgaggtt cgcaagaacc tgatggacat gttcagggat cgccaggcgt
    11161 tttctgagca tacctggaaa atgcttctgt ccgtttgccg gtcgtgggcg gcatggtgca
    11221 agttgaataa ccggaaatgg tttcccgcag aacctgaaga tgttcgcgat tatcttctat
    11281 atcttcaggc gcgcggtctg gcagtaaaaa ctatccagca acatttgggc cagctaaaca
    11341 tgcttcatcg tcggtctggg ctgccacgac caagtgacag caatgctgtt tcactggtta
    11401 tgcggcggat ccgaaaagaa aacgttgatg ccggtgaacg tgcaaaacag gctctagcgt
    11461 tcgaacgcac tgatttcgac caggttcgtt cactcatgga aaatagcgac cgctgccagg
    11521 atataaggaa tctggcattt ctggggattg cttataacac cctgttacgt atagccgaaa
    11581 ttgccaggat cagggttaaa gatatctcac gtactgacgg tgggagaatg ttaatccata
    11641 ttggcagaac gaaaacgctg gttagcaccg caggtgtaga gaaggcactt agcctggggg
    11701 taactaaact ggtcgagcga tggatttccg tctctggtgt agctgatgat ccgaataact
    11761 acctgttttg ccgggtcaga aaaaatggtg ttgccgcgcc atctgccacc agccagctat
    11821 caactcgcgc cctggaaggg atttttgaag caactcatcg attgatttac ggcgctaagg
    11881 atgactctgg tcagagatac ctggcctggt ctggacacag tgcccgtgtc ggagccgcgc
    11941 gagatatggc ccgcgctgga gtttcaatac cggagatcat gcaagctggt ggctggacca
    12001 atgtaaatat tgtcatgaac tatatccgta acctggatag tgaaacaggg gcaatggtgc
    12061 gcctgctgga agatggcgat ctcgagccat ctgctggaga catgagagct gccaaccttt
    12121 ggccaagccc gctcatgatc aaacgctcta agaagaacag cctggccttg tccctgacgg
    12181 ccgaccagat ggtcagtgcc ttgttggatg ctgagccccc catactctat tccgagtatg
    12241 atcctaccag acccttcagt gaagcttcga tgatgggctt actgaccaac ctggcagaca
    12301 gggagctggt tcacatgatc aactgggcga agagggtgcc aggctttgtg gatttgaccc
    12361 tccatgatca ggtccacctt ctagaatgtg cctggctaga gatcctgatg attggtctcg
    12421 tctggcgctc catggagcac ccagtgaagc tactgtttgc tcctaacttg ctcttggaca
    12481 ggaaccaggg aaaatgtgta gagggcatgg tggagatctt cgacatgctg ctggctacat
    12541 catctcggtt ccgcatgatg aatctgcagg gagaggagtt tgtgtgcctc aaatctatta
    12601 ttttgcttaa ttctggagtg tacacatttc tgtccagcac cctgaagtct ctggaagaga
    12661 aggaccatat ccaccgagtc ctggacaaga tcacagacac tttgatccac ctgatggcca
    12721 aggcaggcct gaccctgcag cagcagcacc agcggctggc ccagctcctc ctcatcctct
    12781 cccacatcag gcacatgagt aacaaaggca tggagcatct gtacagcatg aagtgcaaga
    12841 acgtggtgcc cctctatgac ctgctgctgg aggcggcgga cgcccaccgc ctacatgcgc
    12901 ccactagccg tggaggggca tccgtggagg agacggacca aagccacttg gccactgcgg
    12961 gctctacttc atcgcattcc ttgcaaaagt attacatcac gggggaggca gagggtttcc
    13021 ctgccacagc ttgatgaaga tctgagctct taattaatga tcataatcag ccatatcaca
    13081 tctgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
    13141 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
    13201 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
    13261 ttgtccaaac tcatcaatgt atcttatcat gtctggatcc gtttaaacta actttaaata
    13321 attggcatta tttaaagtta catatggcgg ccgccctaag gctcgacctc gaaattctac
    13381 cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg
    13441 ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg
    13501 cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc
    13561 aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca
    13621 cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt
    13681 tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg
    13741 ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtct
    13801 tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct
    13861 tcctcatctc cgggcctttc gacctgcatc catctagatc tcgagcagct gaagcttacc
    13921 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
    13981 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccagat
    14041 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
    14101 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
    14161 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
    14221 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
    14281 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
    14341 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
    14401 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
    14461 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
    14521 cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg cacgacccca tgcatcgatg
    14581 atatcagatc cccgggatgc agaaattgat gatctattaa acaataaaga tgtccactaa
    14641 aatggaagtt tttcctgtca tactttgtta agaagggtga gaacagagta cctacatttt
    14701 gaatggaagg attggagcta cgggggtggg ggtggggtgg gattagataa atgcntgctc
    14761 tttactgaag gctctttact attgctttat gataatgttt catagttgga tatcataatt
    14821 taaacaagca aaaccaaatt aagggccagc tcattcctcc cactcatgat ctatagatct
    14881 atagatctct cgtgggatca ttgtttttct cttgattccc actttgtggt tctaagtact
    14941 gtggtttcca aatgtgtcag tttcatagcc tgaagaacga gatcagcagc ctctgttcca
    15001 catacacttc attctcagta ttgttttgcc aagttctaat tccatcagaa gctggtcgag
    15061 taactttaaa taattggcat tatttaaagt taggcgcgcc ataacttcgt atagcataca
    15121 ttatacgaag ttatgtcgag atatttaaat attagaccca gctttcttgt acaaagtggt
    15181 tgatatctct atagtcgcag taggcggtct aatgaagggt ttctgtcaat atttggagtt
    15241 tttatgggca gaacaatagc aggctcacct ccagaaaatg cttcttttca aatgaatgtg
    15301 ccgtttatag ctgtcaagct tttgtgttcc ctctaagtat gctattaaca ttgggattat
    15361 ctgtccttgg agaaggagaa accactataa cgaactcccc atgtagctat gccaaaaagc
    15421 agtttctctg tgaacatagc tacaacaatt ataaagagat tctatatgtg aagattaata
    15481 tgtaggcaaa aatatttatt tcctttttta ttacctgtgc aaaaagcatc tgtcatgtcg
    15541 tacctaggaa agagtgtgtc gtacttagga aagagaaaga atcctttatg gctgaatttt
    15601 ctttctcttt ttctggctag gttgcccaga atgtaaacta aaggaaaata aatacttctc
    15661 caagctagga gcccccatct accagtgtat gggctgttgc ttctccaggg catatcccac
    15721 tcccgccagg tccaagaaga caatgctggt tccaaagaat attacctcgg aggccacatg
    15781 ctgtgtggcc aaagcattta ctaaggtaag ggctacctga cagtccatcc agaggcagtg
    15841 tgtactcatg gcaagctgtc tggtgaccag gatttgttgc tccgttgttc aggcgacacc
    15901 tgcatttatc ctctttaatt gaaagagtag accaaatgaa aaatgtttat gaaacagaag
    15961 taaggagagc agtagttgcc acagtctaag agatggcgca acgcaattaa tgataacttc
    16021 gtatagcata cattatacga agttatggtc tgagctcgcc atcagttcat cagtggctag
    16081 gagcactcac tgctctgcca gaagtcccaa gttcaattcc cagccaccac atggtggctt
    16141 acaaccacct gtaaggggat ctggcgccct cttctggcct gcaggtaaat gagcagcaga
    16201 gtgcttatac atttactaaa atttaaaaaa agagatgggc agtgggtgcg catgccttta
    16261 atcacagcac tcgggaggca gagacaggtg gatttctgag ttcgagacca gcctggtcta
    16321 cagagtgagt tccaggacag ccagggctac acagagaaac cctgtcttga aacccccacc
    16381 cccccaaaaa aaagttttca agttctacaa aggcattgac agtttgccac agtgtattat
    16441 atagattcta gagaactaga agaaaaggat tcaaaagttg atagcgccag gaaattatac
    16501 attttgtcag aggaatgaaa acattaccac cctgatttga ttgtaatatt tttatattaa
    16561 accaggctcc atatatatgg gcaattaata tattctaaat agaaagtgtc tgggtgtggt
    16621 ggtgcatccc tgtaatctct gacttttttt ttattttatt ttattttatt ttattttatt
    16681 ttttggtttt tttgagactc atcgtttaga aagcagaggt aggaagacct tacatgtcct
    16741 aagcctgtga ctggagctgg tgagatggat cagtgggtaa agacattagc ctaatctgag
    16801 taggatcccc agggtccaga aaacggaagg agagaacaat tctttcaaac tgtcctctga
    16861 catcctgttc tcgtttgtac ttgcttggcc tagggagtgg ctctattcag aggtgtgacc
    16921 ttgttggaac aggtgtgtca ttgtggatgt gggctctaag accctactcc tagcctcctg
    16981 gaaggcagtc ttccaccagc accttcagat gaagatgttg agctttcgtg cctgcctcga
    17041 tactgtcatg ttctcacctt gatgataatg ggctgacctc tgaacctgta atccagcccc
    17101 aatttaatgt tgtctttata gagttgcctt ggtcatggtg tctgttccca gcagtaaaat
    17161 cctaactaag acaaaagttg gtaccaggga ctgaggtatt atttcaatag gttttggaag
    17221 aatgtggatt ttgggacttt ggatttggaa agccgtggaa tgctgtaagt agggtttgat
    17281 aggccatcct agtaggaatg tggaagactt tggggttttt tttggggggg gtggggttca
    17341 agacagggtt tctcttcgta gccctggctg tcctggaact cactctgtag accaggctgg
    17401 cctcgaactc agaaaaccac ctgcctctgc ctgccaagtg ctggaattaa aggcgcgagt
    17461 caccatgcct ggcagcatag cttatcttgc agtgggaatt tcgctgttag agtgaaagcg
    17521 aacatggaaa ataccaaact tttattaagt gacactggcc gctattttcc ttgacatttt
    17581 gcaattgtgg ctgtctacct acctacctgt ttcttttgcc ctttaggcca cagtaatggg
    17641 aaatgccaga gtggagaatc atacggagtg ccactgtagc acttgctact accacaagtc
    17701 gtagcttctg agcgcgcaga gggctgtgct gacggctgct gagccgagcc attcaatggc
    17761 gctgtgtgtc accacctcct ccctaccaga cttctgacgc gcttcagtta cacactgcct
    17821 ctttacggtt acatccctta tacttcagta caatctgcag tctcttctcg ttagggaaat
    17881 atgtagtatt taccacggtt ccgtcagaaa taaagccttt taaatcatca atcccagatg
    17941 tgccgccttc tttgaaaaca cagctgtttg cttttgacaa ggttgatctc ggggttgaat
    18001 tggggttgaa ttgggactca ggggtctgac gctggagaat ctggcttcca tcttaacttc
    18061 tgccagcaag ctgtccattc tgacagaagg ttgtttatca gacagttcgc tgggatggga
    18121 aaaagtggta tggaagttct gtttttactc acttttcata tcttctttac atctatctct
    18181 attttaatac attgcatcaa aatacagata tgctatgatg acaatgcatg cacataaact
    18241 ataggtatat ataccacaaa tatatcatat attgtagata tttgagttga aatgttttca
    18301 ctggtgaaaa cgtacaatca agaaacaaaa agtagggctg gagagatggc tcagtggtta
    18361 agagcactga ctgttcttcc gagggtcctg agttcaaatc ccagcatggg agaacatagt
    18421 ggcccacaac tatctgtagt gagatttgat gctctcttct cagggatctg aagacagctg
    18481 cagtgtacct acatataata aattagtaaa tctttgggcc agagcaggca gggctggaga
    18541 aagaagaaaa agtacctgaa gacagctaca gtgtacttac ataaaataaa tctttttttt
    18601 tttttaaata atagaaagaa acaaaaagta aagtatattt ctctggggat tggggaaggc
    18661 aaagtctctc taagcccaaa atttctactt ggtttatacc ttcccaaaat aaagtctctg
    18721 ttagtcgggc ggtatgactg gggacaggca tcactgtcct cagcgacaat tagacattgc
    18781 tctaaaattc cagaagacca gatgttttta cattacacaa tcaggcagtt ctgggtgtgc
    18841 atgaatacat gacaagagca agctgtcagt gagcagcctg ggtgcgggag gagcaatacc
    18901 aagcactttc actcagttct agccaagtgc tgaggccaag gacattgctc tgcagaggag
    18961 catgcgcaga gtcttgagtt caaccctcaa taccaaaagc aagcaagcag actaacaaag
    19021 cgcttcagag ttctgtccgg gggttgcagt catatttcct gagctttaat atctactgtg
    19081 aaccttactt tgaggaccgt aagatatcca taaagtccta ttattgatga ttgaaattaa
    19141 agctattgct aagttctgtc cctaaaagaa cctgaaaatt taaaacacaa attaaaagct
    19201 tctttttaaa aagccataac cagccaggca gtggtgatgc acgactttaa tcccagcact
    19261 tgggaggcag aggcaggcag atttctgagt tcgaggccag cctggtctac aaagtgagtt
    19321 ccaggacagt cagggctaca cagagaaacc ctgtctcgaa aaacaaaaac aaaacaaaac
    19381 aaaacaaaac aaaaagccat aaccaccaca ggtaacgttt tcaaaaatta ataccaagct
    19441 gctcatggtg taatatgcgt ctagtttcag cacatgtgac acttggtggg tgggggcaca
    19501 ggagttcaag gctagcttgg accacatgag atcctgcctc aaaagcaaga caagtggaca
    19561 gtggtggtgc acacctttaa ttccagcact tgggaggcag aggcaggcag agttcgaggc
    19621 tagcctggtc tacagagtaa ggtccaggac agccagggct acacagagaa gcactgtctc
    19681 aagaaaacaa aaacaggaac aaaaacaaaa acaaaacaaa aaagcaagac aaaaaagtgg
    19741 aaaatacagc cgggcgtggt ggcgcacgcc tttaatccta gtacttggga ggcagaagca
    19801 ggcggatttc tgaggccagc gtggtctaca gagtgagttc caggacagtc agggctacac
    19861 agagaaaccc tgtctcgaaa aacctaaaaa aacaaacaaa acaaacaaac aaaaaaaaaa
    19921 agtggaaaat acataaatat aaatcttacc cgtcacaaac atattgcttt atcactgact
    19981 aaccaatcag ttccttagta gattggtata cattccaggt gccacagtaa aaggtaaaac
    20041 agctgagtat actttttaag acacatcgag gggccaagca tggtggcaca cacctttaat
    20101 cccagcactt gggaggtaga ggcaggtgaa tttctgagtt caaggccagc ctggtctaca
    20161 gagtgagttc taggacagca aggactgcag agagaaaccc tgtctcgaaa aaaaaccaaa
    20221 aacaataaaa ataaaaataa atttaaaaaa tgtttaagac acatcaaagt acacatgaag
    20281 attggtcatt gtttggtttt ggtattgtaa gggtatgaaa atcactgaag gaagacccca
    20341 gactcagata gtatgcaaaa ataaagaaca tttattcttc agaaaaatcc agcatgctgg
    20401 gagcaaccat tctctagacc ccagacagag gcacacaggc cttcttatag ggaagaacat
    20461 tttatggggc agctctctag aaggattagg taatctaaaa tctcattggt gggtactggg
    20521 ggtgagggtc acaggtgatc ggtggtctgc attcctatat catgtgataa aataggactg
    20581 cccttagaag atggcctatt atgagataag ctatttcccc attcttgagg ttgtccccag
    20641 gctgttcttt gggagggggt tttatgatct ttttctggat tttatggtct gtccctggag
    20701 ctgctgtctt aaggcctagt ttctgggact tagggtagct gacttcagac gcaccagaag
    20761 aggacgtctg atctcatgac aggtggttat gagccaccat gtggtttctg ggatttgaac
    20821 tcaggacctt tggaagagca gtcagtgcct taaccactga gccattcatt tctccagccc
    20881 cagatcatcc tatgtttagg gggaattgaa aggatgctca ggttgtaatg gggcatcatc
    20941 cccatgatca tcctgatcgg ccaagttgac gctgtgacca ttaggaggaa acatcgtacg
    21001 tagccagact ggtctccact gctaggtaca gtggtagatg acactaacag actttgctcc
    21061 ggatcaattc acaca
//