LOCUS       214229#pL1L2_GT1_LF2A_nEGFPO_T2A_CreERT_puro#L3L4_pD223_DTA_T_spec        20849 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT1_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 214229
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000108654
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            10221..10263
                     /db_xref="ENSEMBL:ENSMUSE00001231558"
                     /rank="1"
                     /note="ENSMUSE00001231558"
     misc_feature    4275..10341
                     /note="5 arm"
     misc_feature    10369..10393
                     /note="B1 Gateway"
     misc_feature    10406..10453
                     /note="Frt"
     misc_feature    10454..10953
                     /note="mouse En2 intron"
     primer          complement(10522..10543)
                     /note="LRPCR LAR3_EN2"
     primer          complement(10418..10441)
                     /note="FRT3"
     primer          14619..14637
                     /note="seq primer NF"
     misc_feature    14679..14686
                     /note="RE sites"
     misc_feature    16479..16512
                     /note="loxP"
     misc_feature    complement(14687..14718)
                     /note="Rox"
     misc_feature    complement(16439..16470)
                     /note="Rox"
     misc_feature    14719..14740
                     /note="polylinker"
     CDS             15299..15895
                     /note="puromycin N-acetyl transferase"
     misc_feature    15414..15419
                     /note="Mutated RsrII site"
     intron          10954..11328
                     /note="mouse En2 intron"
     misc_feature    11326..11328
                     /note="Splice Acceptor"
     exon            11329..11382
                     /note="mouse En2 exon"
     primer          11229..11250
                     /note="seq primer FCHK2"
     misc_feature    11383..11505
                     /note="1Dleader"
     misc_feature    11506..11557
                     /note="F2A"
     misc_feature    11653..12369
                     /note="EGFP"
     misc_feature    11563..11652
                     /note="3X NLS"
     misc_feature    14435..14673
                     /note="SV40 polyadenylation site"
     misc_feature    13468..14412
                     /note="ERT2"
     promoter        14751..15262
                     /note="PGK promoter"
     misc_feature    16006..16438
                     /note="PGK pA"
     primer          14493..14516
                     /note="LRPCR RAF5"
     misc_feature    12379..12432
                     /note="T2A"
     misc_feature    12429..12429
                     /note="A to G to kill RsRII site"
     misc_feature    12433..13458
                     /note="Cre"
     misc_feature    11344..11383
                     /note="FCHK lf2a_lacz_gt1"
     misc_feature    11344..11359
                     /note="FCHK QC prefix region"
     misc_feature    11360..11377
                     /note="FCHK QC critical region"
     misc_feature    11378..11383
                     /note="FCHK QC suffix region"
     misc_feature    16535..16555
                     /note="B2 Gateway"
     misc_feature    10342..16585
                     /note="Synthetic Cassette"
     exon            16768..16901
                     /db_xref="ENSEMBL:ENSMUSE00000400512"
                     /rank="6"
                     /note="ENSMUSE00000400512"
     misc_feature    16586..20849
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggagctcgg atactgaaag gcaagatgag gtcgcgacat aacgtgggaa
     4321 agaggcggct gcgcggcccg gagccggctg gtctggctgc ctccctgtgt cgggcagggc
     4381 gtgcgagcga gggttccgga gagttctggc ggtaccactc gcgacaagca ggggtgcccc
     4441 ggtcttcagc gagtccctcc gtcgcgcttg gaatgtgggt cggggaagaa gaaaccggcc
     4501 gatcagggcg ctggccttct tgacggccag gaaagagcct aacccggagg agcggccgcg
     4561 tgaggcacgg ggaacccacc cggcgcctgg cgggcgggtc cattttgccg cacaagccgg
     4621 gcaattggca aactgcggat gggcaggtcc actttccttc gggggtgagc ggcctgaggt
     4681 atgggagggc gacgccattt ctcgacgggg gcgggcgggt tgcggattgt tccatggcgg
     4741 gggagacgcc gccgagtggc ggagcgaatg agcacatggc cgcttgagag attcccctcc
     4801 cccagcctgt ttcgcttgtc tgttgtgcaa tctccccctt tgcgagcgcg gctggggctc
     4861 attgtgcgcg aggccgccac cgcccgcggc ctcccacatc cgggtactgt ggaggggggc
     4921 cggccagagg gagtggggga gggcgcgggc ggcatgacgt ggggggaagg ggatggccca
     4981 cccacggaac tcggaggtgg aaggcgggac ttccggtggc cttggtgttc atccaggagg
     5041 tgcacgcgct ttgatccacc agacctagaa catgtgggcc tggctgcaaa gaccgcatct
     5101 cggcgttttg tgtatgagag agagagagag agtgtgaatg tgtgtgtgaa tgaagtcctt
     5161 tacagccgct tgatcttaaa ggagcgtcac tctatgaagt gcctcttgat ggttctttgg
     5221 gagtacatgg gagttggatc gggaatgggc tggttgcttc cactctggtc aaggcatgaa
     5281 gcaaaactag tgctttgaat caacagatcc agagacaatg gccccgacgg gatggagccg
     5341 gaaggcgtca tcgaggtaag accagaacct gcggttccca ctttattttg tcagaacagg
     5401 cacttcctta gcgtgacagt gaccttagac atttggagtg gtagaacaga gcaacctgta
     5461 ggatgaggtg actccagctt tagtgtaaac acgttactcg aagtgcctaa gttttttttt
     5521 ttcctaatcc tgcagagtaa ctggaacgag attgtggata gctttgatga catgaatctc
     5581 tcagagtccc tcctccgtgg tatttatgcc tatggttttg agaagccctc tgccatccag
     5641 cagcgagcta ttcttccttg tatcaagggt aagacttgta ctggactaga ttggactgga
     5701 cagagtggta gggagttggt gggcatagca accgggagga aacatccttc ttggatcttg
     5761 gatttttagt tttgttagta cataggtcat gccttggatg catgggtttg tttccaatta
     5821 gaggctttgt ttttattgct ctacatctag tctaacaagc tgttgtgagt tcataatatt
     5881 ttggcaaaag tttgtttgca aacctctacc atttcattta caaccttttt tctgttactg
     5941 atgtcatgaa gtaaagtttc aaatatcctc tgatcccaag gtgcacatct aattcttgat
     6001 ctggttgtag gttatgatgt gattgctcaa gcccagtctg ggactgggaa aacagctaca
     6061 tttgccatat caattctgca gcagattgaa ttagatctaa aggccactca ggctttggtt
     6121 ctggcaccca cacgtgaatt ggctcagcag gtgagattgg tgtgtttcct atatgactgg
     6181 tttaagagag aagagtacta gtggtgagtg atacaggtag attgagcact gcaagttcaa
     6241 ggccaccctg gtccatagag ctagttccag gacagccagt actatgacgt ttaccttaaa
     6301 aataagacaa ttagaagaag gttgaggaca ttgtcttaat gccactgggt cttgttgaca
     6361 gcttttagtt ctattgagat ttgctgacct aattaccacc aactttacca gcaaaaagaa
     6421 aaaagaaaat gcatcttttg aggaagggga agaggattgg tagaatctat ctagctggct
     6481 gggcaggttt agctgtttat gacacacccc tggcaatggg caaagaggca ccttgtttgt
     6541 ggtgttgttc ttatatcagt caggggcaga gcaaatggca gtcctttgtt catcctagct
     6601 tgatgcctgg cttggtggcc aaagctgttt catgcctagg gcacataaaa tcctgaagct
     6661 aattaagagg attgacgtct tagagccctg tagattttgg gttttgtttg cacatcagct
     6721 ggggatgttg gctctagtgg aacactaagt cttggttctg tggcagggat gaaataatca
     6781 cacacattgt tggtataagg ctatgttcat aatcccagcc tttggatttg gaatgtggta
     6841 gtagggtgga gagtgatttc taagcctttg tagagctggc ctggtgtttt tttacaaggc
     6901 tgcatgtagg ggtggggaaa ttactgtgaa cctgtgtggt gggattcagc actgccattt
     6961 gtggttgtag tgctgggatt gagagagaat tacgtgggta ccgcccgagt taatgtagat
     7021 gttcttctgc attcagatac aaaaggtggt tatggcatta ggagactaca tgggtgcctc
     7081 ttgtcatgcc tgcattgggg gcaccaatgt gcgtgctgag gtgcagaagc tgcagatgga
     7141 agctccccat atcatcgtgg gtacccctgg ccgggtgttt gacatgctta accggagata
     7201 cctgtgtgag taccatgtaa cagttggtgg gcattcccac atgtcatttt cttggatgca
     7261 agttttcctt tagtttagaa ctgagttgct ctgtgatgaa ccccatgcgt gtcatctgag
     7321 cctggcttcc ctgttgttac cttagcccag gtagtgttct acttaccagg gcttttgcct
     7381 ggcttatcag ggaagctttg ggcaaaggat cagtccttgt attctgagag cagacatctg
     7441 ccctttgact gccctcttac caagagattt ccatgtattt cacatttgtc tgatcactat
     7501 gtgttggtgt tttttcttct gccttctagc ccccaaatac atcaagatgt tcgtactgga
     7561 tgaagcagat gaaatgttaa gccgagggtt caaggatcag atctatgaca tattccagaa
     7621 gctcaacagc aacacacagg tggacaaatg gcgaggttga taggacacaa ggggcttggt
     7681 tgtgatctga ggaatgtaca gtgccctcat catggctgct gttttcccca ttaggtagtt
     7741 ttgttgtctg ctacaatgcc ttctgatgtc cttgaggtga ccaagaaatt tatgagagac
     7801 cctattcgga ttcttgtcaa gaaggaagaa ttgaccctgg agggtatccg ccaattctac
     7861 atcaatgtgg aacgagaggt gaggcccagt gcaggaggca gacctggtag taagttgttg
     7921 agtgtaaccc ctgacttctg aatctccagg agtggaagct tgacacattg tgtgacttgt
     7981 atgagacgct gaccatcacc caggcagtca tctttatcaa caccagaagg aaggtggact
     8041 ggctcaccga gaagatgcat gcccgagatt tcactgtttc tgccatggtg agttttaatg
     8101 gctatgtgtc tctgtgtgtc tgtctgtgtt gggagccttg tgccaaatgc ttctacctct
     8161 ttgttttcca gcacggagat atggaccaaa aggaacgaga tgtgatcatg agggagttcc
     8221 ggtctggctc tagcagagta ttaattacca ctgacctgtt ggtgagtaga tgctttcagg
     8281 gaaagaaagg aagacaagtt aagttagtcc aaggtgattc cctctccaaa gggctgactg
     8341 tgtgcccttt tcaggaacgt agcaacttgg aagaaaacca ggcgtgcctg aggtctctgg
     8401 tggacaagaa actcttgttt cctttgccct cctctggctg cctacatgta tgcctactta
     8461 attaggatag attgtatcct ctgatggcta tattctttcc tgacctaggc cagaggcatt
     8521 gatgtgcagc aggtctcctt agtcatcaac tatgaccttc ccaccaacag ggaaaactac
     8581 atccacaggt aagtctaggt ctgacagact aaacaacccc tatgctgaaa cccaagtgtt
     8641 ccatcatcct ttttaccccc ctcagaatcg gtcgaggtgg tcggtttggt cgtaagggtg
     8701 tggctattaa catggtgacc gaagaagaca agaggactct tcgagacatt gagactttct
     8761 acaacacctc cattgaagag atgcccctca acgttgctga cctcatttga ggggctgtcc
     8821 tgcgacctgg ccctagccca gggttcagtc ctggggtggg gctaaggaag agctggaggg
     8881 gggaggggag ggagccaagg gatggacatc ttgtttttgt tttggctttt tttttttttg
     8941 tttcagtttt ttttctctat gaataaatgt cactttttga ggcaaaaaga aggaaccgtg
     9001 aacattttag acaccctttt ctttggggta ggctctgccc caggcgccgt ctccttcccc
     9061 ccccaaacac taatgcattt ccctaaccta gtcacctcgt tcctaaaggc tttcctaccc
     9121 cagccaaatc tccaaaagtg agtcaagggg ctaaaaaaca aggctggcct catttgctgg
     9181 accaaatcta cagggagaac ccctgagtga ggttgtccag ggaattgtcc cctggtgagg
     9241 ggagcagggg agagaaaatg gtagccattt ttacattgtt ttgtatagta tttattgatt
     9301 caggaaacaa acacaaaatt ctgaataaaa ttacttggaa actgcctgtt tgggcttctt
     9361 gtttctttcc cttccctcct acctgctagt gtggatcacc tgcccccata ctagttgatt
     9421 actgaatttg ccatgttgct tcctgcaata ccaaatgacc cacattacta acatttggta
     9481 atttgcctca gtgttaggca caagacatga gtgaaacttg ctgcattaag acttaaactg
     9541 gctgggtatg gtggcgcaag cctttaatcc cagcattctg ccggaggcag aagcaggtgg
     9601 atttctgagt tcgaggctag cctggtctac aaaagtgagt tccaggacag ccaaggctac
     9661 acaaaatacc tgtcttgaaa aaccaaaaaa aaaaaattca actggttggt taggcctagc
     9721 ccagatgatg taggtgtcaa ctcaccttgg ggatgtaggg agatggttgt tctgtagctc
     9781 ttcctgtcct gcaactaaat aaataggcta agctgacctc ttacatctgc cttcggcctc
     9841 ctgtgctagg attggagtgc atgggtgggg ttgcatttct taccaatgag gaaagggctt
     9901 cccatttctc tgctgcattg taagctccca agtagagcaa gttttgctta ggtaagttcc
     9961 gtgtgagtca gctgcctcat tctcacgagg taaccaaggc tttgtaccgc ccactgagaa
    10021 cgtcactgcc aatcacagcc taattgtgaa aaccaatggc ttgagtgggt tgctaaagct
    10081 gaggtgtctg agtcaggttt ggggtgggat tattttagtt aagggaagtg aggcttttca
    10141 tttcctcttc caagagaagg caaaggggat tggattgagg aaggaactgg tgtagcctag
    10201 ctggtctgag catctctgcc atgcggctcc ctgtgtgtct gatcttgcta ggaccgctta
    10261 taggtaagga gaaatgggag gtgggggagg gagggctcat gggcaggagc ctgcacctgg
    10321 gtggccaacc aatctcttac taaggcgcat aacgatacca cgatatcaac aagtttgtac
    10381 aaaaaagcag gctggcgccg gaaccgaagt tcctattccg aagttcctat tctctagaaa
    10441 gtataggaac ttcgaaccct ttcccacacc accctccaca cttgccccaa acactgccaa
    10501 ctatgtagga ggaaggggtt gggactaaca gaagaacccg ttgtggggaa gctgttggga
    10561 gggtcacttt atgttcttgc ccaaggtcag ttgggtggcc tgcttctgat gaggtggtcc
    10621 caaggtctgg ggtagaaggt gagagggaca ggccaccaag gtcagccccc cccccctatc
    10681 ccataggagc caggtccctc tcctggacag gaagactgaa ggggagatgc cagagactca
    10741 gtgaagcctg gggtacccta ttggagtcct tcaaggaaac aaacttggcc tcaccaggcc
    10801 tcagccttgg ctcctcctgg gaactctact gcccttggga tccccttgta gttgtgggtt
    10861 acataggaag ggggacggga ttccccttga ctggctagcc tactcttttc ttcagtcttc
    10921 tccatctcct ctcacctgtc tctcgaccct ttccctagga tagacttgga aaaagataag
    10981 gggagaaaac aaatgcaaac gaggccagaa agattttggc tgggcattcc ttccgctagc
    11041 ttttattggg atcccctagt ttgtgatagg ccttttagct acatctgcca atccatctca
    11101 ttttcacaca cacacacacc actttccttc tggtcagtgg gcacatgtcc agcctcaagt
    11161 ttatatcacc acccccaatg cccaacactt gtatggcctt gggcgggtca tccccccccc
    11221 cacccccagt atctgcaacc tcaagctagc ttgggtgcgt tggttgtgga taagtagcta
    11281 gactccagca accagtaacc tctgcccttt ctcctccatg acaaccaggc tcccaggtcc
    11341 cgaaaaccaa agaagaagaa cgcagatcgc agatcgcaga tctctagagt caccgagttg
    11401 ctttaccgga tgaagagggc cgaaacatac tgtccaaggc ccttgctggc aatccaccca
    11461 actgaagcca gacacaaaca gaaaattgtg gcaccggtga aacagacttt gaattttgac
    11521 cttctcaagt tggcgggaga cgtcgagtcc aaccccggac cggtgcatgt ggaccccaag
    11581 aaaaagcgga aggtggaccc taaaaagaaa cgcaaagtgg atcctaagaa aaaaagaaag
    11641 gtgcacgtgt ccatggtgtc caagggcgag gaactgttca ccggcgtggt gcccatcctg
    11701 gtggaactgg atggcgacgt gaacggccac aagttcagcg tgtccggcga gggcgaaggc
    11761 gacgccacat acggaaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
    11821 ccttggccta ccctcgtgac cacactgacc tacggcgtgc agtgcttcag cagatacccc
    11881 gaccatatga agcagcacga cttcttcaag agcgccatgc ccgagggcta cgtgcaggaa
    11941 agaaccatct tctttaagga cgacggcaac tacaagacca gggccgaagt gaagttcgag
    12001 ggcgacaccc tcgtgaacag aatcgagctg aagggcatcg acttcaaaga ggacggcaac
    12061 atcctgggcc acaagctgga gtacaactac aacagccaca acgtgtacat catggccgac
    12121 aagcagaaaa acggcatcaa agtgaacttc aagatccggc acaacatcga ggacggctcc
    12181 gtgcagctgg ccgaccacta ccagcagaac acccctatcg gcgacggccc tgtgctgctg
    12241 cctgacaacc actacctgag cacccagagc gccctgagca aggaccccaa cgagaagagg
    12301 gaccacatgg tgctgctgga attcgtgacc gccgctggca tcaccctggg catggacgag
    12361 ctgtacaagc ctgcaggcga gggcagagga agtcttctaa catgcggtga cgtggaggag
    12421 aatcccgggc cgtccaattt actgaccgta caccaaaatt tgcctgcatt accggtcgat
    12481 gcaacgagtg atgaggttcg caagaacctg atggacatgt tcagggatcg ccaggcgttt
    12541 tctgagcata cctggaaaat gcttctgtcc gtttgccggt cgtgggcggc atggtgcaag
    12601 ttgaataacc ggaaatggtt tcccgcagaa cctgaagatg ttcgcgatta tcttctatat
    12661 cttcaggcgc gcggtctggc agtaaaaact atccagcaac atttgggcca gctaaacatg
    12721 cttcatcgtc ggtctgggct gccacgacca agtgacagca atgctgtttc actggttatg
    12781 cggcggatcc gaaaagaaaa cgttgatgcc ggtgaacgtg caaaacaggc tctagcgttc
    12841 gaacgcactg atttcgacca ggttcgttca ctcatggaaa atagcgaccg ctgccaggat
    12901 ataaggaatc tggcatttct ggggattgct tataacaccc tgttacgtat agccgaaatt
    12961 gccaggatca gggttaaaga tatctcacgt actgacggtg ggagaatgtt aatccatatt
    13021 ggcagaacga aaacgctggt tagcaccgca ggtgtagaga aggcacttag cctgggggta
    13081 actaaactgg tcgagcgatg gatttccgtc tctggtgtag ctgatgatcc gaataactac
    13141 ctgttttgcc gggtcagaaa aaatggtgtt gccgcgccat ctgccaccag ccagctatca
    13201 actcgcgccc tggaagggat ttttgaagca actcatcgat tgatttacgg cgctaaggat
    13261 gactctggtc agagatacct ggcctggtct ggacacagtg cccgtgtcgg agccgcgcga
    13321 gatatggccc gcgctggagt ttcaataccg gagatcatgc aagctggtgg ctggaccaat
    13381 gtaaatattg tcatgaacta tatccgtaac ctggatagtg aaacaggggc aatggtgcgc
    13441 ctgctggaag atggcgatct cgagccatct gctggagaca tgagagctgc caacctttgg
    13501 ccaagcccgc tcatgatcaa acgctctaag aagaacagcc tggccttgtc cctgacggcc
    13561 gaccagatgg tcagtgcctt gttggatgct gagcccccca tactctattc cgagtatgat
    13621 cctaccagac ccttcagtga agcttcgatg atgggcttac tgaccaacct ggcagacagg
    13681 gagctggttc acatgatcaa ctgggcgaag agggtgccag gctttgtgga tttgaccctc
    13741 catgatcagg tccaccttct agaatgtgcc tggctagaga tcctgatgat tggtctcgtc
    13801 tggcgctcca tggagcaccc agtgaagcta ctgtttgctc ctaacttgct cttggacagg
    13861 aaccagggaa aatgtgtaga gggcatggtg gagatcttcg acatgctgct ggctacatca
    13921 tctcggttcc gcatgatgaa tctgcaggga gaggagtttg tgtgcctcaa atctattatt
    13981 ttgcttaatt ctggagtgta cacatttctg tccagcaccc tgaagtctct ggaagagaag
    14041 gaccatatcc accgagtcct ggacaagatc acagacactt tgatccacct gatggccaag
    14101 gcaggcctga ccctgcagca gcagcaccag cggctggccc agctcctcct catcctctcc
    14161 cacatcaggc acatgagtaa caaaggcatg gagcatctgt acagcatgaa gtgcaagaac
    14221 gtggtgcccc tctatgacct gctgctggag gcggcggacg cccaccgcct acatgcgccc
    14281 actagccgtg gaggggcatc cgtggaggag acggaccaaa gccacttggc cactgcgggc
    14341 tctacttcat cgcattcctt gcaaaagtat tacatcacgg gggaggcaga gggtttccct
    14401 gccacagctt gatgaagatc tgagctctta attaatgatc ataatcagcc atatcacatc
    14461 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
    14521 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
    14581 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
    14641 gtccaaactc atcaatgtat cttatcatgt ctggatccgt ttaaactaac tttaaataat
    14701 tggcattatt taaagttaca tatggcggcc gccctaaggc tcgacctcga aattctaccg
    14761 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg
    14821 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg
    14881 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag
    14941 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg
    15001 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg
    15061 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg
    15121 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcttc
    15181 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc
    15241 ctcatctccg ggcctttcga cctgcatcca tctagatctc gagcagctga agcttaccat
    15301 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
    15361 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccagatcg
    15421 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
    15481 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
    15541 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
    15601 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
    15661 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
    15721 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
    15781 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
    15841 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg
    15901 cccgccccac gacccgcagc gcccgaccga aaggagcgca cgaccccatg catcgatgat
    15961 atcagatccc cgggatgcag aaattgatga tctattaaac aataaagatg tccactaaaa
    16021 tggaagtttt tcctgtcata ctttgttaag aagggtgaga acagagtacc tacattttga
    16081 atggaaggat tggagctacg ggggtggggg tggggtggga ttagataaat gcntgctctt
    16141 tactgaaggc tctttactat tgctttatga taatgtttca tagttggata tcataattta
    16201 aacaagcaaa accaaattaa gggccagctc attcctccca ctcatgatct atagatctat
    16261 agatctctcg tgggatcatt gtttttctct tgattcccac tttgtggttc taagtactgt
    16321 ggtttccaaa tgtgtcagtt tcatagcctg aagaacgaga tcagcagcct ctgttccaca
    16381 tacacttcat tctcagtatt gttttgccaa gttctaattc catcagaagc tggtcgagta
    16441 actttaaata attggcatta tttaaagtta ggcgcgccat aacttcgtat agcatacatt
    16501 atacgaagtt atgtcgagat atttaaatat tagacccagc tttcttgtac aaagtggttg
    16561 atatctctat agtcgcagta ggcggtcttt ttctagacct ccgcctcctc tacaagactg
    16621 ccttatttcc cctttgctga ctgctctcac ctatgggttg gctcctcagc ctttcttcat
    16681 cccctttcct cttccgtcct cccccacctc ccacctttag cccgtccacc actgcagcaa
    16741 ttctcaggga tcccattatt ctgccaggtt tctcttgcaa ccgtgaccag tccctcttgc
    16801 tgcctctcat cattggcctg gtcctcctcg gcctcctcac cctggtgctc atcgccttct
    16861 gcatcacccg cagacgacaa tcaacctacc agcccctctg agcatctgcc ccagtccact
    16921 gtgtaagcca ccaaggagcg cctcatctct tcaccatgcc actgactcaa cagttacctt
    16981 ccttctctgt ctttaggaac acaatgaaga gattttataa taggggtggg gaatcaataa
    17041 acatgaatgg tacctactac tcctttcttt cctcattaag agtacaaaca cttaatgaaa
    17101 tcttttctct tggcttgcct tgtcctgccg gggctaaaag ccacgagttt cttgtcatat
    17161 gccttcctgt gccttcccgt ggctgagtca ccaagagaca gctggcaaga agggacaaaa
    17221 gtaccagaga gtgaccaaat gccttcattg gttcatccct caatgtcctg tgactgctgg
    17281 tgaagctgcc aaaattccct cctccctctg gggccaatgg ctgcctagtc ccttacagaa
    17341 tgaatcattc cgacctgaca ccctgttgct gaccctcggg gcgcagctag gtacaacaca
    17401 actaaaatat gagtaggtgg ctgtattaat ttatttcaaa agtggtgact gaacaatgag
    17461 attccatgtg cactgtggcc ggggctagtt cactgaggag cccactggtg cagccgactt
    17521 ctatgaggct gtgctacgag gaacaacttc aggcctcgca aacacatccc cgagtaccag
    17581 aaggagaggg agcggtggca attgcaatct gcccttgtcc agatataaaa agggattttt
    17641 cagacagcca cccactgtac caaagtataa attctactgt ttatagtagg acatggttca
    17701 acggatggga gccaaggatg ggtgcctttc caatttaaca gtcccatgag gttacagaaa
    17761 ttcttggacc agagccccca cacagcagag gtacatgtga cattatgccc tgaccagcaa
    17821 ctacattccc ctcaccccca aaaacaagga actgagaaac agtttaggaa tgccctacag
    17881 cctgaaccaa gagtaggtgt cctggcagtg tggtggaatg tagatggctc tcttcgccta
    17941 accctagggg ccctttccca gacaaatact ggaattttgc tcgcaccctc tgggttccca
    18001 cctagacaca gcagactgag gtcccctgtt gtgtcagggg ttgctctcct gcagcggagc
    18061 tcgcccctta cggatggctg tgagccacca tgtggttgct gggatttgaa ctcagtacct
    18121 ttggaagagc aatcggcgct cttaaccgct gagccatctc accagcccca ccgtcatcct
    18181 cttaacatct ggacagagga cgaagcaggg gaccagaaag aaggcccagc tcggaatggc
    18241 tcttttgcgg ctctattccc tctggagaaa ccaagaggaa agataaagag agctgcagtg
    18301 cttctgctag cgccattttg aagactcgaa ctcttttccc tttcaacttc aaacactatc
    18361 aagttggccg agattagaca agatggcgta catgcagggg ggacagggcg agggggcagc
    18421 atggaccttg ctttcagaac aagagcgtaa acaaaagtga ggaagtccta acagcgcagg
    18481 cgccaaacgc ggacacgctt taaaattcga acgtcaaggc gcgcacaaaa gcctgcctgg
    18541 gaaggagacc tgtgtcccct agaggaggcg ggcgagcatc acggagaggg aagagcagcc
    18601 ctggcctcgg ggacagcagg gcggggtgtg cgcggcgcgc atgcgcggcg cggataaatg
    18661 gcgggggttg tcccgcctgg cggaagctta gctttccaac atggcggggg aggccgacgg
    18721 gccgttcaaa gggctgctgg tgccaattct tttacctgag aaatgttacg accagctctt
    18781 cgtgcaatgg gacttgcttc atggtgagtt ttatttcagc cttcaaggtc tagctggagc
    18841 agccttgaac cgtaagttgg cgtgacagtg gcgattaatg tcaccgcgaa gctgggcaga
    18901 aggcggttct gacgagggcg gaagtgtttg gggccctgca taggaaaagt gccatatttg
    18961 ggcatgaagg ggtgtctccc gaaaaaaagt taaatttgtg ttttacaatc tcggaagtct
    19021 gtacccaacc ctcatccctt tctgggctgg gagtcgaact cagggcggca tgtgctactc
    19081 tgctaaccag gctctagccc caggctggaa aactcggagc ttttttgtcg tacccccccc
    19141 cccccccccg acagggtata gctctggctg acctggaact cgctctgtag accaggctgg
    19201 cctcgaactc agaaatccac ctgactctgt ctcccaaatg ctgggattaa aggtgtgtgc
    19261 caccaatcct cggcggaaaa ctcgtatttt ataaaacaaa attaaaaccc cagccaggca
    19321 tgtaggcctc ctttcgcccc aagcactcaa ctgacagagg ctcacaaatt ttttttttta
    19381 tcccctctct ccagcaccag cttctggaat ttactatgtg gcacagactg cccttgaact
    19441 ggcaacagtc ctcctgcctc agtcactaga gtgttgggag tatagcactt ttcctttcct
    19501 ttttctttac aaaggccttt gataagcctt ctccatctct ctattctaag tgacttttgg
    19561 cttacaatag tcaagacgcc ttcctgggcc aaatgcccac caggtcaaag attcagtggt
    19621 agagagattg ccaggatcag ggttttgtag gcagcagctc agctcactcg ctgcaatcta
    19681 ttgaaaatca gccctggaca cagggtttga aagaaaaatg ttttttaaaa aaaattagag
    19741 aaattgccag gaactgtggc ccacaccttt aatcccagcc cttgggaggg acccagaggc
    19801 gggagttcaa ggacagccta ggcaactgga tgagttccaa tacaaccagg gctggctaca
    19861 cagagcagcc ttgtctggaa aaaccaagat aagaagtaaa tgataggggc tggagagatg
    19921 gctcagtggt taagagcact gactgctctc ccagaggttc tgagtttaat tcccagcaac
    19981 cacatggtgg cctacaacca tctgtaatgg gatccaatgc cctcttctgg tgtgtctgaa
    20041 gatagatagc tacaatgtac tcacacacat aaagtaaata aataaataaa tctttaaaaa
    20101 aaagaagtaa atgatagaga tagtggaaga acaaggataa atgtcctgga agagtgggga
    20161 caacctgaag gttcaggaat gtatgaatgt gggaatgggg tgggggggga gagaaaagag
    20221 tcagggaacg cctgtgggac tggaggggtg gcttgcaagg ctggcaacct gggtacaatt
    20281 cccggaaccc acagaaaggc aaaaagggag aactggctcc tcaaagtctc tgacctgaac
    20341 acatgcacct atgtgctcac acacaaaata gggttgtttt attttttatt tattttttaa
    20401 tatttattta ttattataca taagtacacc gtagctgact tcagacacac caaaagaggg
    20461 cgtcagatct cattatgggt ggttgtgagc caccatgtgg ttgctgggat ttgaactcag
    20521 gaccttcaga agagcagtca gctctcttac ccactaagcc atctcgccag ccagggttgt
    20581 tttgaaatgt agattttttt tttttatgtg tgtcttttca cttgtatatg tgtgtgtata
    20641 tgtatactat acatgactgc aagggcctga agatgccact ggagctggag ctacgggcat
    20701 ttgtgagtca ctggagaagg atgctaaaaa ttgaattcag atcttctctg caagagcaga
    20761 gatgtgctct taaccactaa gtcatctttc cagtcccaaa tgtttaaaat gtatttctgt
    20821 tgtggtattc ccttatatac actcaggtc
//