LOCUS       KO-first_condition_ready_39256_MGI:1349165        21638 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Sdc2 targeting project(s): 39256
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_Bact_P
COMMENT     design_id: 45978
COMMENT     backbone: L4L3_pD223_DTA_spec
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    complement(4153..4190)
                     /note="Transcription Terminator"
     misc_feature    3826..4079
                     /note="BGH pA"
     CDS             3045..3701
                     /note="DTA"
     promoter        2519..3031
                     /note="PGK promoter"
     promoter        complement(2368..2518)
                     /note="spectinomycin promoter"
     CDS             complement(1357..2367)
                     /translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQA
                     RYAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLL
                     AVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVE
                     VTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVAL
                     VGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIA
                     PKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVV
                     GK"
                     /note="SpecR"
     misc_feature    complement(617..1209)
                     /note="pUC ori"
     misc_feature    complement(319..346)
                     /note="rrnB T2"
     misc_feature    complement(144..187)
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     misc_feature    4275..8720
                     /note="5 arm"
     misc_feature    8748..8772
                     /note="B1 Gateway"
     misc_feature    8785..8832
                     /note="Frt"
     intron          8833..9707
                     /note="mouse En2 intron"
     misc_feature    9705..9707
                     /note="Splice Acceptor"
     exon            9708..9880
                     /note="mouse En2 exon"
     misc_feature    9892..10485
                     /note="ECMV IRES"
     gene            10486..13546
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    13585..13828
                     /note="SV40 polyadenylation site"
     misc_feature    13832..13865
                     /note="loxP"
     promoter        13892..14411
                     /note="human beta actin promoter"
     gene            14437..15231
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    15431..15672
                     /note="SV40 polyadenylation site"
     misc_feature    15689..15736
                     /note="Frt"
     misc_feature    15743..15776
                     /note="loxP"
     misc_feature    9722..9799
                     /note="FCHK std_gpr"
     misc_feature    15792..15812
                     /note="B2 Gateway"
     misc_feature    8721..15842
                     /note="Synthetic Cassette"
     exon            16254..16390
                     /db_xref="ENSEMBL:ENSMUSE00000125096"
                     /type="targeted"
                     /rank="3"
                     /note="target exon 1 ENSMUSE00000125096"
     misc_feature    15843..16567
                     /note="Critical Region"
     misc_feature    16591..16624
                     /note="Downstream LoxP"
     misc_feature    16568..16647
                     /note="synthetic loxP region"
     exon            20634..20769
                     /db_xref="ENSEMBL:ENSMUSE00000125095"
                     /rank="4"
                     /note="ENSMUSE00000125095"
     misc_feature    16648..21638
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatttaag
       61 actggccgtc gttttacaac gtcgtgactg ggaaaacatc catgctagcg ttaacgcgag
      121 agtagggaac tgccaggcat caaataaaac gaaaggctca gtcggaagac tgggcctttc
      181 gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg
      241 atttgaacgt tgtgaagcaa cggcccggag ggtggcgggc aggacgcccg ccataaactg
      301 ccaggcatca aactaagcag aaggccatcc tgacggatgg cctttttgcg tttctacaaa
      361 ctcttcctgg ctagcggtac gcgtattaat tgcgttgcgc tcactgcccg ctttccagtc
      421 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
      481 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
      541 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
      601 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
      661 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
      721 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      781 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
      841 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
      901 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
      961 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     1021 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     1081 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     1141 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     1201 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     1261 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg acgcgtaact
     1321 cacgttaagg gattttggtc atgggtggct cgagggttat ttgccgacta ccttggtgat
     1381 ctcgcctttc acgtagtgga caaattcttc caactgatct gcgcgcgagg ccaagcgatc
     1441 ttcttcttgt ccaagataag cctgtctagc ttcaagtatg acgggctgat actgggccgg
     1501 caggcgctcc attgcccagt cggcagcgac atccttcggc gcgattttgc cggttactgc
     1561 gctgtaccaa atgcgggaca acgtaagcac tacatttcgc tcatcgccag cccagtcggg
     1621 cggcgagttc catagcgtta aggtttcatt tagcgcctca aatagatcct gttcaggaac
     1681 cggatcaaag agttcctccg ccgctggacc taccaaggca acgctatgtt ctcttgcttt
     1741 tgtcagcaag atagccagat caatgtcgat cgtggctggc tcgaagatac ctgcaagaat
     1801 gtcattgcgc tgccattctc caaattgcag ttcgcgctta gctggataac gccacggaat
     1861 gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg agaatctcgc tctctccagg
     1921 ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc cgcgttgttt catcaagcct
     1981 tacggtcacc gtaaccagca aatcaatatc actgtgtggc ttcaggccgc catccactgc
     2041 ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga tggcgctcga tgacgccaac
     2101 tacctctgat agttgagtcg atacttcggc gatcaccgct tccctcataa tgtttaactt
     2161 tgttttaggg cgactgccct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg
     2221 acccacggcg taacgcgctt gctgcttgga tgcccgaggc atagactgta ccccaaaaaa
     2281 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
     2341 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagcttac gaaccgaaca
     2401 ggcttatgtc cactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac
     2461 cttgggtagc agcgaagtcg aggcatttct gtcctggctg gtctagagct gtttatcgaa
     2521 ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc
     2581 ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc
     2641 ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc
     2701 ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa
     2761 gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta
     2821 ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg
     2881 ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga
     2941 aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc
     3001 tcctcttcct catctccggg cctttcgacc tgcaggtcct cgccatggat cctgatgatg
     3061 ttgttgattc ttctaaatct tttgtgatgg aaaacttttc ttcgtaccac gggactaaac
     3121 ctggttatgt agattccatt caaaaaggta tacaaaagcc aaaatctggt acacaaggaa
     3181 attatgacga tgattggaaa gggttttata gtaccgacaa taaatacgac gctgcgggat
     3241 actctgtaga taatgaaaac ccgctctctg gaaaagctgg aggcgtggtc aaagtgacgt
     3301 atccaggact gacgaaggtt ctcgcactaa aagtggataa tgccgaaact attaagaaag
     3361 agttaggttt aagtctcact gaaccgttga tggagcaagt cggaacggaa gagtttatca
     3421 aaaggttcgg tgatggtgct tcgcgtgtag tgctcagcct tcccttcgct gaggggagtt
     3481 ctagcgttga atatattaat aactgggaac aggcgaaagc gttaagcgta gaacttgaga
     3541 ttaattttga aacccgtgga aaacgtggcc aagatgcgat gtatgagtat atggctcaag
     3601 cctgtgcagg aaatcgtgtc aggcgatctc tttgtgaagg aaccttactt ctgtggtgtg
     3661 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
     3721 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
     3781 ggaactgatg aatgggagca gtggtggaat gcagatccta gagctcgctg atcagcctcg
     3841 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     3901 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     3961 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     4021 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     4081 agaaccagct ggggctcgag atccactagt tctagcctcg aggctagagc ggccgagttt
     4141 aaacagataa acggaaaaaa accgggccaa tgttggcccg gtttttttgg atttaggtga
     4201 cactatagat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggaaaatct tataatacct ttcaaaatag cacctctaca tatgagtcaa
     4321 ggatagaata catgagtcta tgggggcaca tttcatattc aaatcatagt accccatgca
     4381 tgcatacaac tgaacaatgt ttccagcccc aagttgcgcc tcttaattga gacagctatc
     4441 aacatattac agaggatgta ggtacatgga ggaggaaatg tgcttggaca cttttcccca
     4501 atatatctta cctggaaatg tttcacctgt gcaagcaatg gctcagtgga caaaccagaa
     4561 atctttggtg ggtagcacat agaaaagtct tagcaagacc tttctttctt agtgtgttta
     4621 ttgcagatga gatttaacta tggtaatagt tattatcctg aatataaata tgtgccagtg
     4681 cagttgacct aggtttcaga atgtcctaga acaaataaag gtcaattgtt tctggtgaag
     4741 atctagactt ataagggtgt ttcagtttga aaatgaccga tctgaaaata tagaggcagt
     4801 agctgaccta gtaacatcct gctgtaaaaa tagttctagt tattcaaggg agtgtgatgc
     4861 attcacatat gcagaacaag tgtgaaagag ttgctctctg aaacccacaa cactattaat
     4921 atcttacact catgatacct cttttgttta gcatctcaaa agtgccttat aactgtcatt
     4981 tcaaaatgcc ttggggaata ataaatatgc atctttgtgg tccccatttc gtcacagatc
     5041 ttagtattca agggtcgtgg gagatcgtta tgggttgctc aactcatttt gctgacttcg
     5101 cccaagattt tgtttacatt tagtataagg tatatgcctc cctgactcat ccagttattg
     5161 gagtgtcaca tttgaagccc tgaacgtact catcatatgg actagataga aatgagactt
     5221 aaatgtgggc aaatttttct agccacagat ataactgtag gcaagttttt gctgaaaaaa
     5281 acaaacaaaa caaaacaaaa caaaacagaa aaagaattat tcttggcttt agaaactatc
     5341 ttgagctttg ctatggatat ttttccactt actagtctgt gtaagtaaaa tttgtgaagc
     5401 actttgtgct gggaaaagcc ttatccatat aattctgtta taaagatatc cctgattcta
     5461 ttgtcatttc tactttcaat tgatacaatc cacagtgcag agagatgaag gagcttgctc
     5521 tcattgatcc atgtgaggct gtcagaaagt aagggaccag gaagcaagaa gtgtggcttt
     5581 agaaaaggcc ttgcctttca gcccttgggg ttttacctct tatgttgaaa atccccattt
     5641 taccattaag aggctttgcc tcacaaatgt ccactgaaat cagtgtattg ctcaatgtac
     5701 cacctgacct tcctcctctc atgctatcca gtaggaaagc aaatgtccca gaacaggatg
     5761 tctaaaggcc cagagagtcc attccagcaa gaagaaggga cctacattac ataaatatag
     5821 attcagaatg ggaaccaaca aactgagaat cttcacaagc atttgtgaag cctttcacac
     5881 caagggctaa acttttaaaa cacacacaga aaaagatggt tttcaagccc aagagagcaa
     5941 gaattcattc acacaactct agaaatttaa aaaaaaaatc attataatgc tgtgattggt
     6001 gacaacagaa ggtactgacc aacagaaata aataatagac ttctggttaa agatgacaaa
     6061 ttgagtagaa agcctgccac attcaagata aagtgaaatt aaaagagtag agcatgaact
     6121 catcagaatg gtgatgcagt aacccaaaca aaacccaaag atgcagtggt tgtgcagcac
     6181 tggtcttggc agctgtgaaa agacagaact ggcagttcag aactgggaag caccaggacc
     6241 gcgctgcaga aggccacagg ggttcagagg tgatggcata aatggggagt gaaagtgaat
     6301 agcctcaggg tcaatagttg aaaatctctc taagaaacaa atagaccctt ccctccatca
     6361 taagttaacc ctccacagat tgattattga aaataaagta tggtattgag caagggagtc
     6421 caaaagactc ctaaaacaat gtaggttgtc ccacagaagt tcaagttcct attgttgaaa
     6481 ccaccatgca ttacagacat gaaacgcaaa ggatttgagc tggataagac ctgaaagcct
     6541 caaaaggaag agggaacaga aactcggaaa aagaagaaaa tgtacagaaa aactaattcc
     6601 aagaggcgag atgttttaat ggtaaaacaa gaaggggaca caacaaaaca ggaatgttcc
     6661 tgagagtgaa actccagtgt tagaaataag aaacttactg gaaaatgaag acatgcaaaa
     6721 gatagttatt tgttgagcac atatttatta agcaccttct ttaatgtggg aacagacatc
     6781 gttcattcta ggaacactac agtaaacaag caaatgaaca aacaaacaaa taaataaata
     6841 tcaatgcttt ttatcatagg gaatcacttc ccttgatccc tttatctgtt tttccaaatt
     6901 tcattctttt ttttttctag taagaaagag tgttaatcca tctagtgctg agttcttggc
     6961 tactgcacgt ctgcagggag ccacccttga acccttccca atagcactaa ttctcttgca
     7021 ttttcaatct cttccactat atgatatttt tgccatctca aaactcagaa ctaatcaaaa
     7081 catgaaaagt gtaaactctc tttctgactg cctttctcat ggaaattctc tgtttactct
     7141 aacattttat agtcaacctt cccaaaaatg gtgtggtgtg gtccttcatc agctcctgtc
     7201 ttacccatgc cctctgtctg ccttcccctg ccatccacta ctgatacttt ttaagatcat
     7261 caatgtcttc catgttggaa atattcacaa atattttatt tgtaaaacgt gttctaaatc
     7321 atcttgattc atttaggata ctataacaaa tattttagag tgggtaactt aaaaaacaaa
     7381 aacaaaaaac agcctaatta tattggtcac agttgtgcag gctgggaagt caaagattac
     7441 atcagtgaat gtggtatata tgagtgtttt tttttttttt ttctgcttcc aaggtgatac
     7501 cttcttgctg catttttatt taatagaata ggcaaactag tttcctctgg cctcttttat
     7561 aaggtgctaa ttcatgcaca aggcagatag aaccctcatt acctaatcac ctctttttcg
     7621 ttgttgttgt tgtttttcaa gacagggttt ctctatatat ctctggctgt tctggagctc
     7681 actctaaacc aggctggcct cgaactcaga aatctgcctg cctctgcctc ccaggtgctg
     7741 ggattaaagg catgtactac cactgtccag cccctaatca cctccttaat atccctttta
     7801 aaaattgtag cggagacaac attctacata tgaacttggg atgggacata acataaggag
     7861 acatcattgt tattaaacac ataggccctg gtgggcgatc aagcatgtgc agacaccaat
     7921 gcacactaaa aatcttaaaa gaggaggact aaagcttagc catgaaggat acttgttatc
     7981 tgccatttta gtggaggagg ataaatatat ctgtttataa tagcacaaaa gtaggagaaa
     8041 atagggaagt tgtaaactgt caaaagaaat agagtgtatc aaggaggtgg tagttcacag
     8101 tgtacaatgg tgaaaggaat gtgttagtca gatttccatt accatcacaa aatatctaaa
     8161 attagcaact taatgaagag gaaatgttat ttggctccca ggtttgaagg ctttccccaa
     8221 ggtataatgg cctgttgctt tggagcctgt agctaggcaa agcatcatgc ttggagtgcc
     8281 tgatgggggt cagttaagaa aagagacaca ggaagaggtc atggtcacag tatactgttg
     8341 aagggactga tcccaatgac tgatcttcct tgaagcctcg ccttttaaag cagcagttct
     8401 ccacctgtgg gttacaaact gtttgagggt tacatatcat atattctgca tatcagatat
     8461 ttacattaca attcataaca atagcaaaat tacagctata aagcaacatc aaaataattt
     8521 catggttgga ggtcaacaca acatgaggaa ctgtatttaa aagttacagc actaagaagg
     8581 atgagaatca ttgtttgaaa ggtctcacta tcttccaata gcattgtgag ttgatagcca
     8641 gtctaacaca tgggctttgg ggaacattta tccagccaat acaagacaag acaaactctt
     8701 taagtatgtt ttaatataaa aaggcgcata acgataccac gatatcaaca agtttgtaca
     8761 aaaaagcagg ctggcgccgg aaccgaagtt cctattccga agttcctatt ctctagaaag
     8821 tataggaact tcgaaccctt tcccacacca ccctccacac ttgccccaaa cactgccaac
     8881 tatgtaggag gaaggggttg ggactaacag aagaacccgt tgtggggaag ctgttgggag
     8941 ggtcacttta tgttcttgcc caaggtcagt tgggtggcct gcttctgatg aggtggtccc
     9001 aaggtctggg gtagaaggtg agagggacag gccaccaagg tcagcccccc ccccctatcc
     9061 cataggagcc aggtccctct cctggacagg aagactgaag gggagatgcc agagactcag
     9121 tgaagcctgg ggtaccctat tggagtcctt caaggaaaca aacttggcct caccaggcct
     9181 cagccttggc tcctcctggg aactctactg cccttgggat ccccttgtag ttgtgggtta
     9241 cataggaagg gggacgggat tccccttgac tggctagcct actcttttct tcagtcttct
     9301 ccatctcctc tcacctgtct ctcgaccctt tccctaggat agacttggaa aaagataagg
     9361 ggagaaaaca aatgcaaacg aggccagaaa gattttggct gggcattcct tccgctagct
     9421 tttattggga tcccctagtt tgtgataggc cttttagcta catctgccaa tccatctcat
     9481 tttcacacac acacacacca ctttccttct ggtcagtggg cacatgtcca gcctcaagtt
     9541 tatatcacca cccccaatgc ccaacacttg tatggccttg ggcgggtcat cccccccccc
     9601 acccccagta tctgcaacct caagctagct tgggtgcgtt ggttgtggat aagtagctag
     9661 actccagcaa ccagtaacct ctgccctttc tcctccatga caaccaggtc ccaggtcccg
     9721 aaaaccaaag aagaagaacc ctaacaaaga ggacaagcgg cctcgcacag ccttcactgc
     9781 tgagcagctc cagaggctca aggctgagtt tcagaccaac aggtacctga cagagcagcg
     9841 gcgccagagt ctggcacagg agctcggtac ccggaagatc tggactctag agaattccgc
     9901 ccctctccct cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt
     9961 gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg
    10021 aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga
    10081 atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa
    10141 acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc
    10201 tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac
    10261 gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag
    10321 gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc
    10381 acatgcttta catgtgttta gtcgaggtta aaaaacgtct aggccccccg aaccacgggg
    10441 acgtggtttt cctttgaaaa acacgatgat aagcttgcca caaccatgga agatcccgtc
    10501 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
    10561 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
    10621 cagttgcgca gcctgaatgg cgaatggcgc tttgcctggt ttccggcacc agaagcggtg
    10681 ccggaaagct ggctggagtg cgatcttcct gaggccgata ctgtcgtcgt cccctcaaac
    10741 tggcagatgc acggttacga tgcgcccatc tacaccaacg tgacctatcc cattacggtc
    10801 aatccgccgt ttgttcccac ggagaatccg acgggttgtt actcgctcac atttaatgtt
    10861 gatgaaagct ggctacagga aggccagacg cgaattattt ttgatggcgt taactcggcg
    10921 tttcatctgt ggtgcaacgg gcgctgggtc ggttacggcc aggacagtcg tttgccgtct
    10981 gaatttgacc tgagcgcatt tttacgcgcc ggagaaaacc gcctcgcggt gatggtgctg
    11041 cgctggagtg acggcagtta tctggaagat caggatatgt ggcggatgag cggcattttc
    11101 cgtgacgtct cgttgctgca taaaccgact acacaaatca gcgatttcca tgttgccact
    11161 cgctttaatg atgatttcag ccgcgctgta ctggaggctg aagttcagat gtgcggcgag
    11221 ttgcgtgact acctacgggt aacagtttct ttatggcagg gtgaaacgca ggtcgccagc
    11281 ggcaccgcgc ctttcggcgg tgaaattatc gatgagcgtg gtggttatgc cgatcgcgtc
    11341 acactacgtc tgaacgtcga aaacccgaaa ctgtggagcg ccgaaatccc gaatctctat
    11401 cgtgcggtgg ttgaactgca caccgccgac ggcacgctga ttgaagcaga agcctgcgat
    11461 gtcggtttcc gcgaggtgcg gattgaaaat ggtctgctgc tgctgaacgg caagccgttg
    11521 ctgattcgag gcgttaaccg tcacgagcat catcctctgc atggtcaggt catggatgag
    11581 cagacgatgg tgcaggatat cctgctgatg aagcagaaca actttaacgc cgtgcgctgt
    11641 tcgcattatc cgaaccatcc gctgtggtac acgctgtgcg accgctacgg cctgtatgtg
    11701 gtggatgaag ccaatattga aacccacggc atggtgccaa tgaatcgtct gaccgatgat
    11761 ccgcgctggc taccggcgat gagcgaacgc gtaacgcgaa tggtgcagcg cgatcgtaat
    11821 cacccgagtg tgatcatctg gtcgctgggg aatgaatcag gccacggcgc taatcacgac
    11881 gcgctgtatc gctggatcaa atctgtcgat ccttcccgcc cggtgcagta tgaaggcggc
    11941 ggagccgaca ccacggccac cgatattatt tgcccgatgt acgcgcgcgt ggatgaagac
    12001 cagcccttcc cggctgtgcc gaaatggtcc atcaaaaaat ggctttcgct acctggagag
    12061 acgcgcccgc tgatcctttg cgaatacgcc cacgcgatgg gtaacagtct tggcggtttc
    12121 gctaaatact ggcaggcgtt tcgtcagtat ccccgtttac agggcggctt cgtctgggac
    12181 tgggtggatc agtcgctgat taaatatgat gaaaacggca acccgtggtc ggcttacggc
    12241 ggtgattttg gcgatacgcc gaacgatcgc cagttctgta tgaacggtct ggtctttgcc
    12301 gaccgcacgc cgcatccagc gctgacggaa gcaaaacacc agcagcagtt tttccagttc
    12361 cgtttatccg ggcaaaccat cgaagtgacc agcgaatacc tgttccgtca tagcgataac
    12421 gagctcctgc actggatggt ggcgctggat ggtaagccgc tggcaagcgg tgaagtgcct
    12481 ctggatgtcg ctccacaagg taaacagttg attgaactgc ctgaactacc gcagccggag
    12541 agcgccgggc aactctggct cacagtacgc gtagtgcaac cgaacgcgac cgcatggtca
    12601 gaagccgggc acatcagcgc ctggcagcag tggcgtctgg cggaaaacct cagtgtgacg
    12661 ctccccgccg cgtcccacgc catcccgcat ctgaccacca gcgaaatgga tttttgcatc
    12721 gagctgggta ataagcgttg gcaatttaac cgccagtcag gctttctttc acagatgtgg
    12781 attggcgata aaaaacaact gctgacgccg ctgcgcgatc agttcacccg tgcaccgctg
    12841 gataacgaca ttggcgtaag tgaagcgacc cgcattgacc ctaacgcctg ggtcgaacgc
    12901 tggaaggcgg cgggccatta ccaggccgaa gcagcgttgt tgcagtgcac ggcagataca
    12961 cttgctgatg cggtgctgat tacgaccgct cacgcgtggc agcatcaggg gaaaacctta
    13021 tttatcagcc ggaaaaccta ccggattgat ggtagtggtc aaatggcgat taccgttgat
    13081 gttgaagtgg cgagcgatac accgcatccg gcgcggattg gcctgaactg ccagctggcg
    13141 caggtagcag agcgggtaaa ctggctcgga ttagggccgc aagaaaacta tcccgaccgc
    13201 cttactgccg cctgttttga ccgctgggat ctgccattgt cagacatgta taccccgtac
    13261 gtcttcccga gcgaaaacgg tctgcgctgc gggacgcgcg aattgaatta tggcccacac
    13321 cagtggcgcg gcgacttcca gttcaacatc agccgctaca gtcaacagca actgatggaa
    13381 accagccatc gccatctgct gcacgcggaa gaaggcacat ggctgaatat cgacggtttc
    13441 catatgggga ttggtggcga cgactcctgg agcccgtcag tatcggcgga attccagctg
    13501 agcgccggtc gctaccatta ccagttggtc tggtgtcaaa aataataata accgggcagg
    13561 ggggatctaa gctctagata agtaatgatc ataatcagcc atatcacatc tgtagaggtt
    13621 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
    13681 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
    13741 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
    13801 atcaatgtat cttatcatgt ctggatccgg aataacttcg tatagcatac attatacgaa
    13861 gttatgttta aacggcgcgc cccggaattc gccttctgca ggagcgtaca gaacccaggg
    13921 ccctggcacc cgtgcagacc ctggcccacc ccacctgggc gctcagtgcc caagagatgt
    13981 ccacacctag gatgtcccgc ggtgggtggg gggcccgaga gacgggcagg ccgggggcag
    14041 gcctggccat gcggggccga accgggcact gcccagcgtg ggcgcggggg ccacggcgcg
    14101 cgcccccagc ccccgggccc agcaccccaa ggcggccaac gccaaaactc tccctcctcc
    14161 tcttcctcaa tctcgctctc gctctttttt tttttcgcaa aaggagggga gagggggtaa
    14221 aaaaatgctg cactgtgcgg cgaagccggt gagtgagcgg cgcggggcca atcagcgtgc
    14281 gccgttccga aagttgcctt ttatggctcg agcggccgcg gcggcgccct ataaaaccca
    14341 gcggcgcgac gcgccaccac cgccgagacc gcgtccgccc cgcgagcaca gagcctcgcc
    14401 tttgccgatc ctctagagtc gagatccgcc gccaccatga ttgaacaaga tggattgcac
    14461 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
    14521 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
    14581 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg
    14641 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
    14701 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
    14761 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
    14821 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
    14881 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
    14941 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat
    15001 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
    15061 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
    15121 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
    15181 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc
    15241 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca
    15301 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
    15361 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccccggat ctaagctcta
    15421 gataagtaat gatcataatc agccatatca catctgtaga ggttttactt gctttaaaaa
    15481 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
    15541 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
    15601 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
    15661 atgtctggat ccgggggtac cgcgtcgaga agttcctatt ccgaagttcc tattctctag
    15721 aaagtatagg aacttcgtcg agataacttc gtatagcata cattatacga agttatgtcg
    15781 agatatctag acccagcttt cttgtacaaa gtggttgata tctctatagt cgcagtaggc
    15841 ggtataaaat gttagagaag acattttcca tgagaaaagc agtcaggtaa aagagagttt
    15901 acacttttca tgttttgatt agttctgagt tttgagatgg caaaaatacc atataacaga
    15961 agagattgaa catgcaagag aagcagggct attaggaagg gtcaagggtg gctccctgca
    16021 gacatgcagt agccaagaac tcagcactgt gtggagatta acactctttc tcactgaaaa
    16081 aaaaaattac catataccca ggggccctac aaacttactg aagtccatag gtctatttgt
    16141 gctagaaact taagcctggt aacttgagag catgattctg aataataatg cctgataatg
    16201 tacagatctg tgtcgtgagt cacatgataa acttatcttc actttcttcc taggagctga
    16261 tgaagacata gagagtccag ttctgacaac atcccaactg attccaagaa tcccactcac
    16321 tagtgctgct tcccccaaag tggaaaccat gacgttgaag acacaaagca ttacacctgc
    16381 tcagactgag gtgcattgtc tggcattgtg aatactgctg tcttttgcct acacagaact
    16441 gttttctgtg agagaagccc cacataaaat tatatgaggg ctaataaccc ttccactgta
    16501 ttgctgtaaa tggctctttg ctctttcttc caacatttta cagttgatct gcttgcggtt
    16561 tttatcagag atggcgcaac gcaattaatg ataacttcgt atagcataca ttatacgaag
    16621 ttatggtctg agctcgccat cagttcatag ctgagagtcc gattttcttt tcctcatgtt
    16681 aaaaaaacat tgtaaacttg acatccattg gaccaggtta aggagggatg atatgaatct
    16741 ttccttaagg tctcaatgag tgtccacacc caccatcagt gttgcataca cttgccctac
    16801 tctcagatac atgtgtacct ggtgtagctg catttatttg ttgtgagacc ttaataagaa
    16861 tgagaccttg gacttgaaac agggaaaaca cctaagtaat gaaaacactg aatttatgct
    16921 ttgattttgt tagtattata ttacaattag ctagccttcc acttatagat acactgttta
    16981 atatggaaat tggatattag aatccagtga aggcctaagc attgactcct ggatctagtc
    17041 aaatttcttg gatcattaga aagattggat gtgaatatta catagcaggc acctgagctc
    17101 ttgttccttg tttcactgtg taagcattag ttgaatttgg tggagcagat gagatcaagc
    17161 cagttcacca agtgagcatg tgtaataatt tagtggtaga ctttacacac attagggatg
    17221 gcatgttgct tatgaaaact taacaacaca agcacctgtt ttttcagttc acccatctgt
    17281 tgatttacaa ataagatgct gccaagctct tgcagcatgc aggttttgtg gcaggctttg
    17341 tgggataaaa agcacccctg tgttagcagg aacataggca ggcttttctt agtagaatat
    17401 ctgttcctcc actttcttga ttttcctgac tcatgagtcc atcaagtcat agcacataac
    17461 tccctgttga caaagctccc tgcagacatg cagtagccaa gagctcagct ctgtgtggat
    17521 taacactctc tctaggctta caaagcattg gcactattgc cagcagtgcc attccacagc
    17581 caggaaccag aaaggcaagg agatactctg agagcactgg ggcagacata cctagcattt
    17641 cctgggagct gttccattgg tttctaagtt gaattaataa aacagatgag gaaaggagag
    17701 acaaaaagca gcaggtgctt ttggtgatag aatcgctgaa tatgtcagag caggcaggta
    17761 tgccagaatt aaatgaagct tgtggaaagc ctgtggaaag atgagcttgg tctctcccac
    17821 agttttatgt gatcttttat aacaatgttt tcaactcaat gaccgttaag actcaatatt
    17881 gcagagtcag aaactctctt ggtaaagttt tggcttccaa tcttcaatag ttttattcta
    17941 aacaaaccgt tgtttggtat aaatttatgg actaatgaca aatatggatt gaaaattgat
    18001 ttaagtttat agaaattggc cctaaaacat aatgaattgg cctttgctaa cagacatttc
    18061 gtggatgagt taagaaatta aagatctctg aacattttaa tgcaaatatt ttatttttag
    18121 ttctatttaa tgagatgaca agaattcatc cattcatggg agttctattc agtgtgcatc
    18181 aaacattgtt ctaggtgtcc atgatgaaga tattgataat gaataacact agcagcaatg
    18241 taattttctg ttttatactt atgtggtacg gctagattaa accttgccat tagagaaaat
    18301 tttgtgtttt aataggtccc agagtggcat tactgtcatg ggtatagata gtctatccac
    18361 tgtgttgtac agctatgtga ctgaagaata gaaagagacc tttcttcatt ttgtgaagcc
    18421 tagttttttt cacagcctct agaatccagt catggattca ataaactttc aattggcttc
    18481 attcaattat gtgtgctccc ttttcataat tacacaaagc accttgaggc ttatgtactg
    18541 actgaacact cttctaagtg ccatttctgt ggagagcctt ctagatcatc acttgaatta
    18601 attcttttgt gtccttattc agaagttgag tcgaagggaa ctgagcaagt caactcttaa
    18661 atgactttag ggacttaaaa tattaacaag taaaaccttg tagcttatct taataataaa
    18721 gataataagc attacataaa cttggggtca aagcttcagt atctttctca cttgctagtt
    18781 ttcactcaca tctttccctg gatgcaaatg ggtatcattc cattccttga tttttttcct
    18841 cttccctttg ctaatttcta aaagcatagc aaccaaagca atgctaatga gcaagaaagc
    18901 atgtgcttcc ttgatgagga ctcaataagc cattctgctg ccctttctgc cagataacca
    18961 gttactgatg gcaatcctat aagaagaaaa tggaaagaac ctacaaaata ctgccaacag
    19021 gcttctctca tctgcattac ttgggcacag caagacttaa aggaccaacc atgttttcta
    19081 tactcagaca gaaaacagtg aaacttgaat ttatatttat acagttataa aaattataat
    19141 tatttataga aaatatggca gcttaagatt tatgaagtat agggtttctt gtatcttggg
    19201 aaaaacctct atcacatgtt attactgtgt ttggtggaaa ccttgtgtgt tctgatcact
    19261 aaaagtagta ccaattggtt cacattgatc tctagagttc aagtgaatcc acctggtcta
    19321 gactgcggtc ttttctagac ataaagatga ctttgaaaca gtccaagaaa atgaactatt
    19381 ttcttagacc ttttgccttc tttaatagga tgtattccag ccttcacaaa acagctgaat
    19441 gattcacatg actctgctta agatggctgg gaattcatag gaaaaactgg aataggtttg
    19501 gctagggagg agattcagtg tctactgatt ttgtagctgg tattgtgtaa acctgacagt
    19561 agaggtatca gagaggaagg agcctcagtt aaggaattgc ctctacaaga tccagatata
    19621 aggcattttc tcaattcgtg atcaatgaag gagggtgttc ctatccctgg gctggtggtc
    19681 ctgggttcta taaaaaaaag caagctaagc aagccagagg aaacaagtca gtaagcagca
    19741 ctactccata acttctccat cagctcctgc tgctaggatc ctgccaagtt tgagtacctg
    19801 tcctgacttc cttcaataat gaatagcaat atgaaagtgt gagccaaaaa caaaacaaaa
    19861 aacaaacaaa aaaaaaacaa caaaaaaaaa aaaacccttt ctccccaact tgctttctgg
    19921 tcatggtgtt tggccacagc aatagaaacc ctaataaaga cacagcttaa gagttgacaa
    19981 tgttttccta tcagtagtgt tagccaatga gaaagaaact cacctgttag aaaacagcag
    20041 ctcattagaa tcttaacttt tctaaatcag gccttctcaa accacaccat catctgaggg
    20101 actttaaata acaataaaaa tcagcagacc aactgaacag aatactcaaa gggtgtgtgt
    20161 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt atcagtggag attggctgcc tcatggacct
    20221 agaagtcact tagcaagctg gccagagcct taagattcta ccatgccccc aacaccgtgc
    20281 cttcatgtga cttcaagggg acaaactcag gttctcatgc ttgcaaacaa gcaagtactt
    20341 tattgccagg gatcttccca acccagaaag taaagattct tagaattgtc aactaaaaca
    20401 attattcata tcattcttgt catctttgct tcaatgatac tagaaccaca agctacatgg
    20461 gtccaaactg ggactcacca actggcaaaa taaacatcat acacattatt attcatgata
    20521 tttactaaac ttacttttat ggaaaaaaac ctgggaatat acttagtatc gtttcttctt
    20581 cccagcacgt atcaaaagag agataattgt gttttctttc ttccccacac cagtcacctg
    20641 aagaaactga caaggaggaa gttgacattt ctgaggcaga agagaagctg ggccctgcta
    20701 taaaaagcac agatgtgtac acggagaaac attcagacaa tctgtttaaa cggacagaag
    20761 ttctagcagg tgagttgcta ggagtgtcaa gtgggaagga acttcaagag gccttcctgg
    20821 gataggtgac attcagaaag cttgctgcac attactggcc ttcaggaagc aatctccagg
    20881 ctttttattt cagaatgaca ggtgagaagt taggactact ttgtctattt tctgttaact
    20941 cctccagtct gtccctcagc cagccattga taagaggcta acatgggaga caatttcagg
    21001 ttacagtcca tcatggcaag gacatcaaaa tggcaggagc ttgaagtagt aaattgacta
    21061 ggcaaagaac tgttgagtgt ggaggcaggg tgggagatgt cggtcagaca gtcataattc
    21121 aggtgatctt ccaagttcaa aaccaacttc ggagaaggaa aaggtacaaa tgctgctgct
    21181 gctgctgaca tgataagaat tttgcctcta attgaaattc acctgatgaa agtacaggtt
    21241 gagcctcact gacctgagca tctgcaatat tctaaacctc caaaactttt taagacctga
    21301 catgatgcca caaatgtcct caggatgtgt gcctataaca ggagcacact acagaaactt
    21361 tgtcccatga atagaattat ttgttaactt gtgtaagctt tcctatgaac tatgtgtata
    21421 aaatatacgt ggaaatataa atatatttcc tgtttagact tgtatctgat ccctgataac
    21481 ttaatccatg tatgtaaata tttaaaaaaa atccagagat ggaaacactc ctggttccag
    21541 atatttctga aaagaaatag ccactgaagt ctgtactgat atttgtttta aagaggaaga
    21601 tgcgcaagat ttaacaacat ttaatttata caatgctc
//