LOCUS       KO-first_condition_ready_125521:125371_MGI:87889        23208 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Chrna5 targeting project(s): 125521:125371
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT0_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 178345
COMMENT     backbone: L3L4_pD223_DTA_T_spec
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            5947..6098
                     /db_xref="ENSEMBL:ENSMUSE00000266890"
                     /rank="2"
                     /note="ENSMUSE00000266890"
     exon            8387..8431
                     /db_xref="ENSEMBL:ENSMUSE00000534631"
                     /rank="3"
                     /note="ENSMUSE00000534631"
     misc_feature    4275..9844
                     /note="5 arm"
     misc_feature    9872..9896
                     /note="B1 Gateway"
     misc_feature    9909..9956
                     /note="Frt"
     misc_feature    9957..10456
                     /note="mouse En2 intron"
     primer          complement(10025..10046)
                     /note="LRPCR LAR3_EN2"
     primer          complement(9921..9944)
                     /note="FRT3"
     primer          14108..14126
                     /note="seq primer NF"
     misc_feature    14168..14175
                     /note="RE sites"
     misc_feature    15968..16001
                     /note="loxP"
     misc_feature    complement(14176..14207)
                     /note="Rox"
     misc_feature    complement(15928..15959)
                     /note="Rox"
     misc_feature    14208..14229
                     /note="polylinker"
     CDS             14788..15384
                     /note="puromycin N-acetyl transferase"
     misc_feature    14903..14908
                     /note="Mutated RsrII site"
     intron          10457..10831
                     /note="mouse En2 intron"
     misc_feature    10829..10831
                     /note="Splice Acceptor"
     exon            10832..10871
                     /note="mouse En2 exon"
     primer          10732..10753
                     /note="seq primer FCHK2"
     misc_feature    10872..10994
                     /note="1Dleader"
     misc_feature    10995..11046
                     /note="F2A"
     misc_feature    11142..11858
                     /note="EGFP"
     misc_feature    11052..11141
                     /note="3X NLS"
     misc_feature    13924..14162
                     /note="SV40 polyadenylation site"
     misc_feature    12957..13901
                     /note="ERT2"
     promoter        14240..14751
                     /note="PGK promoter"
     misc_feature    15495..15927
                     /note="PGK pA"
     primer          13982..14005
                     /note="LRPCR RAF5"
     misc_feature    11868..11921
                     /note="T2A"
     misc_feature    11918..11918
                     /note=" A to G to kill RsRII site"
     misc_feature    11922..12947
                     /note="Cre"
     misc_feature    10847..10872
                     /note="FCHK lf2a_lacz_gt0"
     misc_feature    10847..10862
                     /note="FCHK QC prefix region"
     misc_feature    10863..10866
                     /note="FCHK QC critical region"
     misc_feature    10867..10872
                     /note="FCHK QC suffix region"
     misc_feature    16024..16044
                     /note="B2 Gateway"
     misc_feature    9845..16074
                     /note="Synthetic Cassette"
     exon            16455..16564
                     /db_xref="ENSEMBL:ENSMUSE00000266843"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 1 ENSMUSE00000266843"
     exon            18386..19214
                     /db_xref="ENSEMBL:ENSMUSE00000534637"
                     /type="targeted"
                     /rank="5"
                     /note="target exon 2 ENSMUSE00000534637"
     misc_feature    16075..19412
                     /note="Critical Region"
     misc_feature    19436..19469
                     /note="Downstream LoxP"
     misc_feature    19413..19492
                     /note="synthetic loxP region"
     exon            20493..21858
                     /db_xref="ENSEMBL:ENSMUSE00000584408"
                     /rank="6"
                     /note="ENSMUSE00000584408"
     misc_feature    19493..23208
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggaagtagg aaatttatgc atttgagaac ttttaggggc aaagtctgtt
     4321 tgactcctga ttatgtaaag cggtgaggag agagtggtgt caagtgtaac acatagtttt
     4381 ttagactatc acacctcgga cggacccgct cactgcgatg ggaaacctgg cagggccatt
     4441 tgggggaagg ttgtgaatat gcccttggaa ttctctttct aatagagagc aaagcttcca
     4501 ggacccccac tgctgagctt cgttaagacc taggacccaa agtagtttta tacaaatata
     4561 tagtaaaaca cacctaaata attctgtcaa tattttaata tataaagtta agccaactaa
     4621 agtacaggcc ttaatatttc tagttaatca atggacttta cttcagtata tctggtcttt
     4681 ttttttttta tgaagcaaag ttagaattta ttaagcctat tcgcttagaa tttttacatc
     4741 aatccctatc tatatccctg acagttgaaa agcctttact ttttgttttg gcagcatgag
     4801 gcattggacc caaagctaca ttcaagttct tgtagtctac cataagaaac aaatcaaata
     4861 aataaataat taaaaaaaaa acccagccac tggtatttaa atgttaagct attctttatt
     4921 aactctgaat gtttattaag gaattcagga tattttgttt ttctcacatt tttatataat
     4981 tttatttgat ttatttttat ttttagtttt taaattattt atttacttta tatccctgtt
     5041 gcaatttccc ttccctcctt tctcccctcc ctccttccct cctagtccct ccctcgcccc
     5101 tgccaccgca atttagaata tttctatagt gactaatctc atttaactaa gaattatatg
     5161 tgaaccagag tatctctaac gtgaccatag tgtttgtatt agtatttcat ttgaaaagtc
     5221 acattagggg actggagaga tggctgttaa ggacatactg tccttgcaga ggacctgagt
     5281 ttgattccag catccacatc aagcagccta caacggtctg caactccagc tctgggagaa
     5341 atctgacacc tctgaccact atgggcactg tattcatgga gtgcacatac cctcgccata
     5401 cgcacacata caccttggac acacacacac acacacacac acacacacac aaatacactc
     5461 tacacacaca cacacacaca cacacacaca cacacacaca cacactccac acacacactc
     5521 tctccattct ctctccctct ccctctccct ctccctctcc ctctccctct ccctctccct
     5581 ctccctctcc ctctccctct ctctttctct ccacaaacat acacactctc cacacacaat
     5641 ttaaaataaa aagaaatctt aaaaattagc tggatggatg caatggtgga tgcctataat
     5701 cccagtactt tggaggtgga ggcaggagga tcaggagtcc aaggctgtac ttggtaacag
     5761 ggaattcagg gccagccaat ttcaaacaaa caaatttatt tgatatcctg tttcaaacaa
     5821 acaaataaac caaaaccaat aataagttaa attttaattg gattattgat aacattgagt
     5881 ttcttagagt atggctattt gggggagagc agccagcaaa ctaattccta aaaacttgtt
     5941 atgcagggtt gcctgagcta tcctctgctg caaaacatga agacagtttg tttagggatt
     6001 tatttgaaga ctatgaaaag tgggttcgtc ctgtggaaca cctgagtgac aagataaaaa
     6061 tcaagtttgg ccttgcaata tctcagttag tggacgtggt aagtatccat gccattacat
     6121 gttccactta gcacttaggg aaaaccaatt tttttcccag gtgtgagtta gccactagag
     6181 gttgccagag tgaccaaatg cagcccttcg ctcaaggggc tcttcaggaa acaattgcta
     6241 gtatgccaca ggcataggaa taccatttat atgttgttgt gaagtttaga aattgtttta
     6301 tttcatttgc tattaatagg tctatcctta atgaaattta aacatatcaa aacattttat
     6361 gttgaagttc cccttaaatg tgtgtaaggc tgttctggga ggtctgagta gtagtctaac
     6421 aatagaatca tagattatct taacattgtt tcaagatcac acttcaatgg acagtggtgg
     6481 cacaggcctt taatcccagc actagggagg cagaagcagg tggatctctg agttcaaggc
     6541 cagcctggtc tacaaatcgt gttccaggat agctagggct aaacagagaa accttgtctc
     6601 aagaaaaaaa aatcatactt caaacatatc aggagggagc actcagaggt taagactgct
     6661 cttgcggagg acctagagtt gcttcccagc acctacatag tggtcacatc cactggcaac
     6721 ccccatttta ggggatctgg tgtcctcttc tgacctccat aggcaccagg catgcacatg
     6781 gtgcacatac atacttgcag gcaaagcact gatacacata aataaaacaa tatttccaga
     6841 gagattttat gtgttgttca tcaggggtga attacctgct tctctgttta ccctaagaaa
     6901 ctcacacttt caaaactaat ttcttcttgg tctgataatg ccaacaattg tttttagtat
     6961 aatgaacaaa tatttactta tattaaaagt aacactagtg tgcctttatg ttatgattac
     7021 agaagtaaca catgattttt agagggtttt taaagtgtaa aaatgtacaa ataaacagat
     7081 tcgtgttcta ccgtccattg gtgactaatg ttaacaattg atagtgaggt aggagcgggg
     7141 ttcacacata taatcccagc actttggagg ttaaatcagg agaatcatga gttctgggtt
     7201 atcctctgct gcacagagag tttgagccag tcctgagacc ctgtatttta aaaaatacaa
     7261 tgtgttgtat ttcttgccat tttttccctt gtgcctgttt tacaaatttg ggattcttat
     7321 ttctttattt tattttgtgc atatgagtgc tttggctgca tacgtttctg tgcatcacga
     7381 gcatgtctgg tgcctaaggg catggtgaac ctctgtgtgg gactgggagt tgaacccagg
     7441 tcccctacaa gaacaacaaa tgctcttacc cactgagcca gctctccagc ccctggcatg
     7501 tgtttttgat atgattttgc tgactgcttt tctctcaaat cctcctataa tttacttgca
     7561 taaacttgtc tagtcttata gtgatgccgt gtttgtacat gaatgtctga attctccttc
     7621 ctttaagaag ggcgggctga gtacggtagc acacactctc ccaccctttt agagctaagg
     7681 gatgttgtac tcatatctta gagcctctct tggatttgag gaaatatagt agttgagacc
     7741 ctgaaaaata aatatagaat cgctagtggt atgctttagg gtagtttacc tgaactacag
     7801 aatgtttaga ggaacaatag cagattaatc ttattttctt gaaaagcaga tgaattagca
     7861 aaatgttcat tccatgtgga gaggaacgtt tgtattgaaa tgtaccgatt gtattttttt
     7921 taagcttaag gaccatatta ttaaaatttg agagaggggg gagaggggag agaggaaaag
     7981 agagagggag gagagagagg agagggagga gagtggggaa ggcggggaga gaagcagggc
     8041 agggcagctg taaatctcaa caggtgcagg gaggagggag atggagagga aagccacacc
     8101 ttttgagtta gggtcagcaa tgaaggtctg tgagcagcta gagagagagg gcagggtctt
     8161 cagagaaggc cagaatgtca gggaaggcat acttgggggt tcagttatta ctcaagaact
     8221 agataggaga aaatgaagtt acattttttt gagccagaac tcagaaaagt agacaggctt
     8281 acgagcgatg cctgtcactg tcagcaactg ccacaaatat acacactctt aaagaattat
     8341 tcctatgtaa cagaaataag caaccttact tctttttttt taataggatg agaaaaacca
     8401 gctaatgacc accaacgtct ggttgaagca ggtgtgtata gcaatcacac aggcacggag
     8461 cgagcagctg gctacactgt tcattacagg ctacagcaag gaggagcttg cgtgtgtgcc
     8521 ctgtggattg tcaggttgag aacgctgaga ataaatagat ggaactattg tactcattgt
     8581 gcatgtatac tgtgcatatt tgttttgaac cagggtcttg ttatacagct tcagtcttcc
     8641 gtcttcatcc tcccaagttc tggaattacc actgtgtctg ctttagttga ctttttttgt
     8701 gggtctgata tgtgttaagc actggagaaa ctgcagtaat ggagcagaca gaactcactg
     8761 ttcccccaga aacctcatcc aagtggaaag agagtgagcc atagtgcctt agagaccgac
     8821 agtctgagag aggtcacgtg gcaaagagga tggagagtgt tggctactat acttgttagc
     8881 aaaaagaaac acccaaccaa gccataaaaa aatgactgaa aaggcaaagt ttgtacaaag
     8941 tctcaaggga gatgatgcaa gccttatggg cagctagggg acaagactct tctgggtaga
     9001 ggggccagca agtcaaaggc ccagaggcag gattgcatct agtaagggaa ccagtgtggc
     9061 tagaggtgtg tgagccatgg ggaggggtat ggagatgggg tcagaggagc aacaagaagc
     9121 cgctagaata ttcaaagcca ctagagtatt agtttatagg acaccacaag gatttggagt
     9181 tttactttca tttgttgtta ttttaaaaaa gatttatttc tatttatgtc tgagttattt
     9241 agtttgcata tatacacaca tacacgaaat gtccaaggag gccagaagaa ggcattgcat
     9301 cccctgggcc tggcattaca gagggttgta agctgctgtg tgggtgctgg gaactggacc
     9361 tgctcttctc caggagcagc gagtgctctt cgccctgagc catctgccta gccccaggat
     9421 tctggctttt aatttgattg caatgggaca ggataaggat gcaactaatt attttattat
     9481 cattgttaac atgctaactg tgcatgttac tgggattcac tgtgatattt tcatgcatgc
     9541 agacctcatg cattgaccgt gtccatccac cctgtaatca ccctcctcct cctccagccc
     9601 cctccgcatt ccccagtgac cccctttgta attaattatt tttatggaca gagcaccttt
     9661 tacatgaagg gcttttaaat tctgggccac cattcttgtg tctctggctg ctgttgagtg
     9721 cccccccccc ccattgttta ttctttctct gtctctgatt atttataccc accaccagtt
     9781 ccctaatccc agacttacaa aagacaggtt gatgttgttt ttttgtctgt ttgctgcgca
     9841 ggggaaggcg cataacgata ccacgatatc aacaagtttg tacaaaaaag caggctggcg
     9901 ccggaaccga agttcctatt ccgaagttcc tattctctag aaagtatagg aacttcgaac
     9961 cctttcccac accaccctcc acacttgccc caaacactgc caactatgta ggaggaaggg
    10021 gttgggacta acagaagaac ccgttgtggg gaagctgttg ggagggtcac tttatgttct
    10081 tgcccaaggt cagttgggtg gcctgcttct gatgaggtgg tcccaaggtc tggggtagaa
    10141 ggtgagaggg acaggccacc aaggtcagcc ccccccccct atcccatagg agccaggtcc
    10201 ctctcctgga caggaagact gaaggggaga tgccagagac tcagtgaagc ctggggtacc
    10261 ctattggagt ccttcaagga aacaaacttg gcctcaccag gcctcagcct tggctcctcc
    10321 tgggaactct actgcccttg ggatcccctt gtagttgtgg gttacatagg aagggggacg
    10381 ggattcccct tgactggcta gcctactctt ttcttcagtc ttctccatct cctctcacct
    10441 gtctctcgac cctttcccta ggatagactt ggaaaaagat aaggggagaa aacaaatgca
    10501 aacgaggcca gaaagatttt ggctgggcat tccttccgct agcttttatt gggatcccct
    10561 agtttgtgat aggcctttta gctacatctg ccaatccatc tcattttcac acacacacac
    10621 accactttcc ttctggtcag tgggcacatg tccagcctca agtttatatc accaccccca
    10681 atgcccaaca cttgtatggc cttgggcggg tcatcccccc ccccaccccc agtatctgca
    10741 acctcaagct agcttgggtg cgttggttgt ggataagtag ctagactcca gcaaccagta
    10801 acctctgccc tttctcctcc atgacaacca ggctcccagg tcccgaaaac caaagaagaa
    10861 gaacgcagat ctctagagtc accgagttgc tttaccggat gaagagggcc gaaacatact
    10921 gtccaaggcc cttgctggca atccacccaa ctgaagccag acacaaacag aaaattgtgg
    10981 caccggtgaa acagactttg aattttgacc ttctcaagtt ggcgggagac gtcgagtcca
    11041 accccggacc ggtgcatgtg gaccccaaga aaaagcggaa ggtggaccct aaaaagaaac
    11101 gcaaagtgga tcctaagaaa aaaagaaagg tgcacgtgtc catggtgtcc aagggcgagg
    11161 aactgttcac cggcgtggtg cccatcctgg tggaactgga tggcgacgtg aacggccaca
    11221 agttcagcgt gtccggcgag ggcgaaggcg acgccacata cggaaagctg accctgaagt
    11281 tcatctgcac caccggcaag ctgcccgtgc cttggcctac cctcgtgacc acactgacct
    11341 acggcgtgca gtgcttcagc agataccccg accatatgaa gcagcacgac ttcttcaaga
    11401 gcgccatgcc cgagggctac gtgcaggaaa gaaccatctt ctttaaggac gacggcaact
    11461 acaagaccag ggccgaagtg aagttcgagg gcgacaccct cgtgaacaga atcgagctga
    11521 agggcatcga cttcaaagag gacggcaaca tcctgggcca caagctggag tacaactaca
    11581 acagccacaa cgtgtacatc atggccgaca agcagaaaaa cggcatcaaa gtgaacttca
    11641 agatccggca caacatcgag gacggctccg tgcagctggc cgaccactac cagcagaaca
    11701 cccctatcgg cgacggccct gtgctgctgc ctgacaacca ctacctgagc acccagagcg
    11761 ccctgagcaa ggaccccaac gagaagaggg accacatggt gctgctggaa ttcgtgaccg
    11821 ccgctggcat caccctgggc atggacgagc tgtacaagcc tgcaggcgag ggcagaggaa
    11881 gtcttctaac atgcggtgac gtggaggaga atcccgggcc gtccaattta ctgaccgtac
    11941 accaaaattt gcctgcatta ccggtcgatg caacgagtga tgaggttcgc aagaacctga
    12001 tggacatgtt cagggatcgc caggcgtttt ctgagcatac ctggaaaatg cttctgtccg
    12061 tttgccggtc gtgggcggca tggtgcaagt tgaataaccg gaaatggttt cccgcagaac
    12121 ctgaagatgt tcgcgattat cttctatatc ttcaggcgcg cggtctggca gtaaaaacta
    12181 tccagcaaca tttgggccag ctaaacatgc ttcatcgtcg gtctgggctg ccacgaccaa
    12241 gtgacagcaa tgctgtttca ctggttatgc ggcggatccg aaaagaaaac gttgatgccg
    12301 gtgaacgtgc aaaacaggct ctagcgttcg aacgcactga tttcgaccag gttcgttcac
    12361 tcatggaaaa tagcgaccgc tgccaggata taaggaatct ggcatttctg gggattgctt
    12421 ataacaccct gttacgtata gccgaaattg ccaggatcag ggttaaagat atctcacgta
    12481 ctgacggtgg gagaatgtta atccatattg gcagaacgaa aacgctggtt agcaccgcag
    12541 gtgtagagaa ggcacttagc ctgggggtaa ctaaactggt cgagcgatgg atttccgtct
    12601 ctggtgtagc tgatgatccg aataactacc tgttttgccg ggtcagaaaa aatggtgttg
    12661 ccgcgccatc tgccaccagc cagctatcaa ctcgcgccct ggaagggatt tttgaagcaa
    12721 ctcatcgatt gatttacggc gctaaggatg actctggtca gagatacctg gcctggtctg
    12781 gacacagtgc ccgtgtcgga gccgcgcgag atatggcccg cgctggagtt tcaataccgg
    12841 agatcatgca agctggtggc tggaccaatg taaatattgt catgaactat atccgtaacc
    12901 tggatagtga aacaggggca atggtgcgcc tgctggaaga tggcgatctc gagccatctg
    12961 ctggagacat gagagctgcc aacctttggc caagcccgct catgatcaaa cgctctaaga
    13021 agaacagcct ggccttgtcc ctgacggccg accagatggt cagtgccttg ttggatgctg
    13081 agccccccat actctattcc gagtatgatc ctaccagacc cttcagtgaa gcttcgatga
    13141 tgggcttact gaccaacctg gcagacaggg agctggttca catgatcaac tgggcgaaga
    13201 gggtgccagg ctttgtggat ttgaccctcc atgatcaggt ccaccttcta gaatgtgcct
    13261 ggctagagat cctgatgatt ggtctcgtct ggcgctccat ggagcaccca gtgaagctac
    13321 tgtttgctcc taacttgctc ttggacagga accagggaaa atgtgtagag ggcatggtgg
    13381 agatcttcga catgctgctg gctacatcat ctcggttccg catgatgaat ctgcagggag
    13441 aggagtttgt gtgcctcaaa tctattattt tgcttaattc tggagtgtac acatttctgt
    13501 ccagcaccct gaagtctctg gaagagaagg accatatcca ccgagtcctg gacaagatca
    13561 cagacacttt gatccacctg atggccaagg caggcctgac cctgcagcag cagcaccagc
    13621 ggctggccca gctcctcctc atcctctccc acatcaggca catgagtaac aaaggcatgg
    13681 agcatctgta cagcatgaag tgcaagaacg tggtgcccct ctatgacctg ctgctggagg
    13741 cggcggacgc ccaccgccta catgcgccca ctagccgtgg aggggcatcc gtggaggaga
    13801 cggaccaaag ccacttggcc actgcgggct ctacttcatc gcattccttg caaaagtatt
    13861 acatcacggg ggaggcagag ggtttccctg ccacagcttg atgaagatct gagctcttaa
    13921 ttaatgatca taatcagcca tatcacatct gtagaggttt tacttgcttt aaaaaacctc
    13981 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
    14041 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
    14101 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
    14161 tggatccgtt taaactaact ttaaataatt ggcattattt aaagttacat atggcggccg
    14221 ccctaaggct cgacctcgaa attctaccgg gtaggggagg cgcttttccc aaggcagtct
    14281 ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct
    14341 cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc
    14401 gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg
    14461 tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc
    14521 gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc
    14581 gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc
    14641 aggggcgggg cgggcgcccg aaggtcttcc ggaggcccgg cattctgcac gcttcaaaag
    14701 cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcatccat
    14761 ctagatctcg agcagctgaa gcttaccatg accgagtaca agcccacggt gcgcctcgcc
    14821 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
    14881 gccacgcgcc acaccgtcga tccagatcgc cacatcgagc gggtcaccga gctgcaagaa
    14941 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
    15001 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
    15061 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
    15121 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
    15181 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
    15241 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
    15301 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
    15361 tgcatgaccc gcaagcccgg tgcctgacgc ccgccccacg acccgcagcg cccgaccgaa
    15421 aggagcgcac gaccccatgc atcgatgata tcagatcccc gggatgcaga aattgatgat
    15481 ctattaaaca ataaagatgt ccactaaaat ggaagttttt cctgtcatac tttgttaaga
    15541 agggtgagaa cagagtacct acattttgaa tggaaggatt ggagctacgg gggtgggggt
    15601 ggggtgggat tagataaatg cntgctcttt actgaaggct ctttactatt gctttatgat
    15661 aatgtttcat agttggatat cataatttaa acaagcaaaa ccaaattaag ggccagctca
    15721 ttcctcccac tcatgatcta tagatctata gatctctcgt gggatcattg tttttctctt
    15781 gattcccact ttgtggttct aagtactgtg gtttccaaat gtgtcagttt catagcctga
    15841 agaacgagat cagcagcctc tgttccacat acacttcatt ctcagtattg ttttgccaag
    15901 ttctaattcc atcagaagct ggtcgagtaa ctttaaataa ttggcattat ttaaagttag
    15961 gcgcgccata acttcgtata gcatacatta tacgaagtta tgtcgagata tttaaatatt
    16021 agacccagct ttcttgtaca aagtggttga tatctctata gtcgcagtag gcggagaata
    16081 cttaagtgta tggggtgtgt aataaatcca actctccttc caccaaatca gggcacagta
    16141 cagctgggaa ggggaacgtg tttcctaaac ctggacttta gggactggtc catggcttca
    16201 ggttttgagt acagtcagct ggtgggagaa gaggggccaa gtagagaagc ttgaatgtat
    16261 cgcatgagcc tcacctgtgc cccatcagga ccacagcgag aggcattgtg cagagaatgg
    16321 cttattatta gtcgaggttt actgaattag agcagaaatg attcatgcct aagttctggg
    16381 tatgacaaaa agtatagttt aaacttattt tattattgct tgattgaaat ggaggtgact
    16441 ttctcatttt aaaggaatgg atagatgtaa agttaagatg gaatcctgat gactatggtg
    16501 gaataaaaat tatacgtgtt ccttcagact ctctgtggat cccagacatc gttttgtttg
    16561 ataagtaagt catattctaa gtattatttc ctatttccaa aagaaaactt gcattgctac
    16621 taggcaccag taattttttt tcctcttgtc tgcaaattgt ttagctatga aatcaaatga
    16681 cttgactttg tgtctctttg ttgcatgtgt gctgtggatc ctttgccttt tttggtgtgt
    16741 tttttttttt tcatcataaa agtgttatct gatttctgaa ataagaaaaa ggacatggcc
    16801 aatgtggagt aaagaaaagt ggtatgcatt atctgataga gataatggct gtggttcatt
    16861 tacacataca gtaaactcat gtgcttggca gtgtgttcac ggcgagcttc cgtgctgggc
    16921 gccacccatt gtactttgcc taaggtagtt acacttgtct atgatcacaa agctctctct
    16981 ctctctttct ctttctctct ctctctctct ctctctctct ctctccctcc ctccctccct
    17041 ccctccctcc ctccctctct ctctctctct ctctctctct ctctctctct ctctctcttt
    17101 gagacagggt ttctctgtat agccctggct gtcctggaac tcactctgta ggccaggctg
    17161 gcctcaaact caaaaatccg cctgcctctg cctcccgagt gctgggatta aaggcgtgcg
    17221 ccaccaggta cagtaaggtt tcaccccaag accctggcat atccacctac cccttattca
    17281 agtgagactt ccaggtttca ggggattttt tttttagttg aggttacaat ttatggtcaa
    17341 ccttgttttc taatattaca taaatacatt gtcatgatgg cacaacccat ttttccacgg
    17401 tgaggagttt ggggttcatt tttgatggta atgtcagtta ccagctgtat tccccctccc
    17461 ctcccctccc agggccatct cagagtaaat gtgagagttt gcatgtatcg taaagcaccc
    17521 tgtctcattt gcaaaaggct tttagagcaa acactatgca aaaaatgatc ttttgaagcc
    17581 tttacttctg tgttccctga tatggaagga tggagaaggc tcgtaaggta acacgaggca
    17641 gcttctggca cattctctca gtaaccacaa gcaccttgtc ctgtttgcac aaaagctttg
    17701 gcatgcagac agatcaaatg ctgtgcaaaa gcaatctttt gaaacctttt gcttccacat
    17761 taatatgaaa gcatgaacaa agcttgcaag gtagcacgat gcagcaccat gagcctcagc
    17821 tgctctctgt atgaaaacat tgttatgact cagcatagat agattgcacg gcacggtgaa
    17881 aagcccacag gacttgaaga tacagagcaa gactatgaag gaatccggtg cttctgcctg
    17941 cagctgtgcc tgcccagcac aggagcatcc caggtgtgcc ctgcatggtg cagatactcc
    18001 ttcctcttag ggtagaaagc atctattatt ttggtggagg agaaagtcta gggatgtgga
    18061 ttcgggctct gcctttctgt gtgcccagta tataaacagc ccgatcctgt ttgtatgtaa
    18121 agtggcatct tttcaatgac taaaatctca acattttgag aaacttggat ttgcccggct
    18181 cacaatacaa agggaggaat aggctcagtc ccagcagaat tcttacatga tagacagatg
    18241 tgaccccgtg ggtggagcag gcattggggc agacagtaat ttcaaactca tttcacacta
    18301 ttacccccag gatcctttta aattaggctt atcaaatgtg tcctcgggct gtttgacttc
    18361 ggcgctatta ttttgtttct tctagtgcag acggacgttt cgaaggggcc agtacgaaaa
    18421 cagttgtcag gtacaatggc actgtcactt ggacgcagcc agcaaactac aaaagttctt
    18481 gcactataga tgtgaccttt ttcccatttg atctccaaaa ttgctccatg aaattcggct
    18541 catggacgta tgatggatcc caggtcgata taatcctaga ggaccaagat gtcgacagaa
    18601 cagacttttt tgacaatgga gaatgggaaa tcatgagcgc aatggggagc aaggggaacc
    18661 ggacggacag ctgctgctgg tacccctgca tcacctactc cttcgtgatc aaacggctgc
    18721 ctctcttcta caccctgttt cttatcatac cctgcatcgg gctctcgttt ctgactgtgg
    18781 ttgtcttcta tctcccttca aacgaaggtg aaaagattag cctgtgcact tccgtgctgg
    18841 tctctctgac tgtcttcctg ctggtgattg aggaaattat accgtcatct tccaaagtca
    18901 tacctctgat cggggagtac ttggtgttca ccatgatctt cgtgacccta tccattatgg
    18961 tgactgtctt tgccatcaac atccaccacc gctcttcttc cacacacaat gctatggcgc
    19021 cgtgggttcg taagatattt ctccacaagc ttcccaaact gctctgcatg agaagtcatg
    19081 ccgataggta cttcactcag agagaagaag ccgagaaaga cggtggacct aaatctcgga
    19141 atactttgga ggccgcgctc gattgcattc gctacatcac gagacacgta gtgaaagaga
    19201 acgacgtccg cgaggtttgt ggcgagcata aacacccccg caaagtgggg tatctacatg
    19261 aatctcaccc tccatttccc cagagcatct tgaatgaccc tgaagaaagt ttagtagtat
    19321 gcatttgaag ggatagtaat attgacttag ttctttggct ctggaatagt ttactgattt
    19381 agatttatct tccataactt tgtttctgaa ttgagatggc gcaacgcaat taatgataac
    19441 ttcgtatagc atacattata cgaagttatg gtctgagctc gccatcagtt cacttaagat
    19501 actttgcata gagatatctt ttgtagcttg tttacaaatg aatattagta aaattgaaaa
    19561 gagcaaggtg catggctata attctaccat tcaggggctg aggcgggagg attgaaagtt
    19621 agagtctata ttgatgtact ttaggaagtt catggttagc atgggctaca tagggagatt
    19681 atgtctaata aaagcaaagt gaaataaata gccataaaaa taaagaaaag gaataaggtg
    19741 tgcccatgtg ttattttata ctttagagtt ttcaggaaag cagtaatagt atccctgcct
    19801 tcctccctcc cttcccctct tcatcccctt ctctcctcct tccttccttc cttccttctt
    19861 tccttttttt tttttttttt gagacagtca ctttatagtc ctggctagac tggaatttgg
    19921 tatgtagacc aggctggctt cagacttaca gaaattcccc cttttggatt aaagtcgtgt
    19981 tccattatgc caaagccccc accgttcact ttttaaagaa aatttcacta tgtagctcag
    20041 gttagccttg aactgtcaat ccttttactc cagcctcctg aatgctgaga tttcagcaaa
    20101 tactgccatg tgcaggttat gactttgaac taactctttg ttattgttcc aaggtgctgg
    20161 ccttgaactt ctgatcctcc tgcctcagtc ttccaagtag ctggtactac atgctcctac
    20221 cactgtggcc agtttacttt taacaaatta gtacttgtta actgtagtat tctatggcag
    20281 tataattgaa ttattgtagg gtttaagata ttgatggaat cttaaaaggt aagaatttat
    20341 tgaatataaa ttccaaattt aaatttagaa tgggatttaa taaattatac tagtctgtct
    20401 tagagatctg ggcataggag acagcaaatg attatctctc tgttttttat cctaaaatac
    20461 agctttatat atgtatttat tttttttaac aggttgttga agattggaaa ttcatagccc
    20521 aagttctcga tcggatgttt ctatggacgt ttcttctggt ttcaatcatt gggacgctag
    20581 ggctttttgt tcctgttatt tataaatggg ccaatataat agtcccagtt cacattggaa
    20641 acacaattaa gtgaaaccaa gaaattacac tggacttagt gagcagtcaa gcagctctta
    20701 ggacacgtat gccattatga aaatatttac aatttgatat aggcagtaac agattagcag
    20761 tttctgacat tggcttaatg ttgtccatta gaactgtagt aatagccttg agtaccaaca
    20821 ctgtccgcct gcactagtga aggcctacca tcgcatcctg gcaaatccta ctaacttgca
    20881 accagtgacg aagggcatct ttggagtact ggaaaatgca actatatttg aagactgttt
    20941 aaagcttttc tccccacatt tagtaaaagc atatatttgt gtggttttga attttcagaa
    21001 tgggtcttgt taaattgtcc aggctggccc taaacctcct gagtagctag gactatgggt
    21061 gtgctccatt ttgccctgtt ctgtattcat ggatataaaa cacatcatac ttttatagga
    21121 gataagccga ttacttggtt taataataac tatttttgtg aagagacacc atgatcaaca
    21181 actcttataa aggaaaacat ttaattgggg ctggcttact ggttctgagg tttagtccat
    21241 tattgtcatg gcagaagcat ggcagcatac aggcagacat aatgttgaag acaagagttt
    21301 tctacatctg gattggctgg cagcaagaag agagagtgac ccactggaca tgacttgagc
    21361 ttctgaaacc tcagaaccca cctcctgtga cacactttct tcaacaaggc cacacctcct
    21421 catagtgcta ctccttggtg atcaagcatt caatctatgg gtctatgaga accatttcta
    21481 ttcacaccac ccttgatagg atgctattct tactgacatt tttaatatgt gaaaaatggt
    21541 aactagaaac tccttaagtc tactttattc ttcatatggc aatatggaat ggcttttgtg
    21601 ctaactaact ttgcagctta ctgtccactg aaaacatata acaggcatct cctgcagaca
    21661 ttctttgtaa tgactttata atcctgcagt gacacatttt ttgtaatgac ttcagtcgcc
    21721 ttgtccatat tctttggtta ggagagctac ttatttatct ctgatttcag aataatactg
    21781 cagttgtaca gattcccgtc tcaaaacggg atgttatgtc tctgtcttgg tagttctgtg
    21841 gaaaaaatca ttctgggctg gatatggtgt ctcatacctg tcatctctac tttcaggagg
    21901 caggggcagg ggaattgttg tgagttgtag gtcatcctgg gttacagtag gacaccctgt
    21961 ctcaaaagcc aaacaaacaa aaattgccct tctgagttga tgtaagcaca ctgtcacatc
    22021 ttataatgtc tcaatcatct ttcaccccaa ttataatggt caaatcttag agcaaaacgc
    22081 tttagatata gtctagctag cctattacta ggtatgatat cagtcagtac tgtgtaatgt
    22141 aacatgggta aagtagttaa acttcatccc tagtgcccca actcagctat aacccaaaag
    22201 cttgcgtttg attcacaact cttagttgta aagttagcaa catctttggt tattagtaat
    22261 atattatcaa caatttaaat tcaggtcagc attctttgaa aggcaggatg gaccctgtca
    22321 agtttaattt tattgtgatt attgtgttta tgactgttag aatgagtgaa agtctgtact
    22381 gtagtttagt aaatgtccct caaaggctca ttgttgaaag cttggtcatg gtggttttgg
    22441 gaggtggtgg gatcttcagg agtataggct ctggcagggg aaagttaggt ctctaggagc
    22501 gtgcccttga ggatattgag accccagctc ttccttttgc ttcccagaca ccatgaggta
    22561 ggcaacttgg ctctgctttg tgtctcccag tgttatatac atcttgacac tgacctcaaa
    22621 gcaagacccc atcatggact aaggcctctg caagcatgag caacaacaaa gctttctctt
    22681 aattagctca agtattttct tgattatctc aagtgttttt cccataatag cagaaagcta
    22741 atagaatatt agtatcctga agtgaaatct ctggtgtgac ttttgctgtg gagtctactt
    22801 ctagtcattc tagactttaa aaaggtagga atagaacaaa gagataattc tgcccaagta
    22861 cagcttggaa accagtgagt ttactgtggc tacttatagg aacatggagg gaggggtttt
    22921 tccatggaag aaaccatgga aagcccacct tagcctgggt aacaactttt acaacctgca
    22981 ggtaacagtc ctgcagtttc ctctttcctg ttgtttgcta tttttataaa ttcaggaatg
    23041 aatcttggga gtctggtaag tcctgtgagt ttcatgaatc tggtgagcat ccctctcccc
    23101 cctagaaggg aatgtttcaa cctggaggaa acagccacac aaaagggtag ttattgtgat
    23161 aataaataat ggggtagctc tgaagccttt gaattttacc cgtagaaa
//