LOCUS       232702#pL1L2_GT0_LF2A_nEGFPO_T2A_CreERT_puro#L3L4_pD223_DTA_T_spec        22411 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT0_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 232702
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000037814
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            9196..9509
                     /db_xref="ENSEMBL:ENSMUSE00000648741"
                     /rank="1"
                     /note="ENSMUSE00000648741"
     misc_feature    4275..10228
                     /note="5 arm"
     misc_feature    10256..10280
                     /note="B1 Gateway"
     misc_feature    10293..10340
                     /note="Frt"
     misc_feature    10341..10840
                     /note="mouse En2 intron"
     primer          complement(10409..10430)
                     /note="LRPCR LAR3_EN2"
     primer          complement(10305..10328)
                     /note="FRT3"
     primer          14492..14510
                     /note="seq primer NF"
     misc_feature    14552..14559
                     /note="RE sites"
     misc_feature    16352..16385
                     /note="loxP"
     misc_feature    complement(14560..14591)
                     /note="Rox"
     misc_feature    complement(16312..16343)
                     /note="Rox"
     misc_feature    14592..14613
                     /note="polylinker"
     CDS             15172..15768
                     /note="puromycin N-acetyl transferase"
     misc_feature    15287..15292
                     /note="Mutated RsrII site"
     intron          10841..11215
                     /note="mouse En2 intron"
     misc_feature    11213..11215
                     /note="Splice Acceptor"
     exon            11216..11255
                     /note="mouse En2 exon"
     primer          11116..11137
                     /note="seq primer FCHK2"
     misc_feature    11256..11378
                     /note="1Dleader"
     misc_feature    11379..11430
                     /note="F2A"
     misc_feature    11526..12242
                     /note="EGFP"
     misc_feature    11436..11525
                     /note="3X NLS"
     misc_feature    14308..14546
                     /note="SV40 polyadenylation site"
     misc_feature    13341..14285
                     /note="ERT2"
     promoter        14624..15135
                     /note="PGK promoter"
     misc_feature    15879..16311
                     /note="PGK pA"
     primer          14366..14389
                     /note="LRPCR RAF5"
     misc_feature    12252..12305
                     /note="T2A"
     misc_feature    12302..12302
                     /note=" A to G to kill RsRII site"
     misc_feature    12306..13331
                     /note="Cre"
     misc_feature    11231..11256
                     /note="FCHK lf2a_lacz_gt0"
     misc_feature    11231..11246
                     /note="FCHK QC prefix region"
     misc_feature    11247..11250
                     /note="FCHK QC critical region"
     misc_feature    11251..11256
                     /note="FCHK QC suffix region"
     misc_feature    16408..16428
                     /note="B2 Gateway"
     misc_feature    10229..16458
                     /note="Synthetic Cassette"
     exon            16821..16973
                     /db_xref="ENSEMBL:ENSMUSE00000385822"
                     /type="targeted"
                     /rank="2"
                     /note="target exon 1 ENSMUSE00000385822"
     exon            17508..17600
                     /db_xref="ENSEMBL:ENSMUSE00000320895"
                     /type="targeted"
                     /rank="3"
                     /note="target exon 2 ENSMUSE00000320895"
     exon            17778..17862
                     /db_xref="ENSEMBL:ENSMUSE00000393749"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 3 ENSMUSE00000393749"
     exon            17959..18182
                     /db_xref="ENSEMBL:ENSMUSE00000648736"
                     /type="targeted"
                     /rank="5"
                     /note="target exon 4 ENSMUSE00000648736"
     misc_feature    16459..18315
                     /note="Critical Region"
     misc_feature    18339..18372
                     /note="Downstream LoxP"
     misc_feature    18316..18395
                     /note="synthetic loxP region"
     misc_feature    18396..22411
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggagagtat tttctgaggg aaagcacatg caaatcagca cacattattc
     4321 tagagacagt gtgtatgagt gtgttggggt ggggcagaag gtccctgggc aaacagagaa
     4381 agacctggag gaagcacact ggccggtctc tgcacagtcg tcccctgcag gctggacctt
     4441 gattggcctg tgtctttgta atctgtcttc tggagtatac acacacccac ctggctaata
     4501 gggccccatg agagtgcaga agctgaatgg gccccctatg gctggagagt agcagctatc
     4561 cttgttgtga gttttgttcc tggggctcta tgaacaggag tgaaacctcc tgctgggtct
     4621 tagagacctc agtgtcctct tctccatgtt ccactctcat ttgtgcagta tagacagtca
     4681 cccacagctt cttccatcac tcttgcccac ccaagcagac aactccttca ggcatatcct
     4741 ggtagcatgg tagggcgaga gctgccttct catcccagga agaggtcagg gaaaatctgg
     4801 ggacagggat gggaacaggg tggcaaagtg agaaggcaaa gggaaattgg gggtggtaat
     4861 ggacagcaag gagaagtcat gctcaaaatg gctgggttta gccaacaaaa ggggatggcg
     4921 gcccaagagg ctgaagagaa ggccagtgtt tattaagaca ggtggtcaga gacatctaag
     4981 aagaagagcg acagccagag gtcagatcct aggtaggaag tcttggaaat ggtgcccttg
     5041 gaaaggagac cacagaacat tctttaattt gaggtagcac acacacatca gggaaattcc
     5101 catcatgcca tcccacagag acagaattgc agatgctatt actacgaccc ccgcccggaa
     5161 agtgcaagga acaaagttta cccaatcagc tgccaggctg ataatgatga tcattacaac
     5221 ttccatgagg gtagcattta atcagctttt gggaacactt tcagggttgg gtttttttgg
     5281 ttgttgttat tggttttatt ttttttttct tttggttttt cttttctttt tttttaatct
     5341 caagtcactc cctctaaaag atagtggaaa taaatttgaa taaacaaaac aggtttgctg
     5401 aaaataaaac caggacacct cagctgctcc agtcagcctc tctcagaagg cctgtcaggc
     5461 aggggtagta cctgtgactg gtgagcagtg tccggcatgt accaggtgag tggccctgat
     5521 gtctgacaga ggaatggctg agagagaaag gagtagagat ggtattgagt gtgtaggaag
     5581 gctaggcttc caggcttcca ggcttctggt ctcagggagc tgcaggaggt agggggtggg
     5641 gcttgtatag tatgggggaa ctgcaagtga cctgcgagta gctttctcat tggcttttgc
     5701 tcataggctg ttgcattctt ggcaatgatc gtgggaaccc acaccgtcag cttgcggatc
     5761 caggagggct gcagtcactt gcccagctgc tgccccagca aagagcaaga acccccggag
     5821 gagtggctga agtggagctc tgcatctgtg tcccccccag agcctctgag ccacacccac
     5881 cacgcagaat cctgcagggc cagcaaggat ggccccctca acagcagggc catctctcct
     5941 tggagctatg agtgagtgtc cgtagtcccc tcctcaatgt tctcatggat cccccagggc
     6001 atggatgagg ggccggggga gttccaccca atttgaccta ttttaattaa ttaaacagac
     6061 cagataggtt tgggagctac acaggcctga atttgactct cagctctgtc ctcattgtgt
     6121 tgggtgatgt tttcaaagtt ccttccttcc ttctttcctc tagattatat tctagaggct
     6181 aatacatagg tcaaatgcaa caatgtatgt aaagcctgta tttatattga ttctgtttct
     6241 tgtttagtct ccaccataac tcatatgtct ctaaagtatc aggtgacata gacacaaagc
     6301 accactatgc caaatgctgt cactcgaccc tggtggggca ggtgggaggc cagatcccta
     6361 ggtttctcat ctttaattaa aaccccagct ctcttaatct tttacacagc gtgtcacata
     6421 cacattgtaa ctctttattt atgacatgat atgaaactgg gacagagaaa ccatttacaa
     6481 attagggtct tagggattcc aaacatgtga tttcattcct gtatactcac atggttatac
     6541 agcccccccc ccgtgtgtct cagtttctta gccttatctt tgaggggtta tgaagggatt
     6601 cctggtatag ggagctggtc agctttaact gatattgtgt ctaagacacc agttcataat
     6661 gccttggagt tgtctcagag gctacgtgga agatctgctt ggggagtggg ggtctcagct
     6721 acacctgtcc actttttgga aaggcagtaa taacccttga tgaccactta gcagaccctt
     6781 tgacatatgc tctcacaaca ctggtcccac acgctgtttc tcaggaaaaa aaaaagtaat
     6841 ggtgtcaagt taaatttgtg aaaggttaca gagccccatc ccttgaagat tcattgctct
     6901 ccatacatta caattggcaa gaagtctaca aaaagaagcc gttagtgtcc cccaaacttg
     6961 ttagatcgca gaagcttttg ttttacagac agacaggcag acaaataaca aaaacaaaac
     7021 ctcaaggtat ttattatgga cgcttgcggc agttggaccc caatttccca gggctcatat
     7081 gggaaatggt tctaccacac tggggcttcc cgctaggcta ctggatgcaa tatatatatt
     7141 tctttccagt ttttgttttg atctgggctt tgttgttgtt gccacaaatc ttttctgaat
     7201 tggtaagaaa aaaaagtctt tcccatgacc taaatgtgcc tgtaatccac tcttgggctg
     7261 tgactggaga gtggacatga agcagctctc cctggggagt acatagaaca aaaggaaaca
     7321 cttgttctct gtgtattctt ttatatgtgc ctgtcgactg tgtggcacag gttagcctca
     7381 aacttatggc tgtctttgcc tcggcctctc aagtgctagg gttacaggca cgtgccacct
     7441 cacagcagtt tttaattttg taatagctgt aatttagata aacatcataa aacggaatac
     7501 cttcctaaga agcctcatgc atatcagacc ggtctgcatt ggtctggtgg aggctggcag
     7561 gacagtcttc gccttcccct gtgctgtaag aagctgtcat gtctatggcc tggcattgag
     7621 agccactagg aaagccacgt ttttgttgcc tacctaaatt aatagtggga ggaaatattt
     7681 ctcagagaga aaggggtagc aaagttacac agtattgggt gccagaacag atttaagaag
     7741 ttgagtcccc caaccacgcc atttgctccc tccagcgtct ctaccccacc tgccccttgc
     7801 ttctggccta atcagctctg tccccacagg ttggacaggg acttgaatcg ggtcccccag
     7861 gacctgtacc acgctcgatg cctgtgccca cactgcgtca gcctacagac aggctcccac
     7921 atggacccgc tgggcaactc cgtcccactt taccacaacc agacggtctt ctaccggcgg
     7981 ccatgccatg gcgaggaagg tacccatcgc cgctactgct tggagcgcag gctctaccga
     8041 gtctccttgg cttgtgtgtg tgtgcggccc cgggtcatgg cttagtcatg ctcaccacct
     8101 gcctgaggct gatgcccggt tgggagagag ggccaggtgt acaatcacct tgccaatgcg
     8161 ggccgggttc aagccctcca aagccctacc tgaagcagca ggctcccggg acaagatgga
     8221 ggacttgggg agaaactctg acttttgcac tttttggaag cacttttggg aaggagcagg
     8281 ttccgcttgt gctgctagag gatgctgttg tggcatttct actcaggaac ggactccaaa
     8341 ggcctgctga ccctggaagc catactcctg gctcctttcc cctgaatccc ccaactcctg
     8401 gcacaggcac tttctccacc tctccccctt tgccttttgt tgtgtttgtt tgtgcatgcc
     8461 aactctgcgt gcagccaggt gtaattgcct tgaaggatgg ttctgaggtg aaagctgtta
     8521 tcgaaagtga agagatttat ccaaataaac atctgtgttt aaagtggtct tatttgtctt
     8581 tggcatctgt ggtacttgag catcctttag tcttcagctg ggtccttcct tctccctagg
     8641 tccttctcca ctctgcaact ttccctactt tccttgggtg tccaccacat ccacccttcc
     8701 ccttcctcct ctcccctccc ctggtccatc cctgaacaca agggtggggg tcacacagct
     8761 caacatggag gagagacaga aggccattga ggcactgcag aaggcacaca gcgccccctt
     8821 gtatccccaa caatcctcct ttgtcctctg gttctggctg ctgcagcttg gtccagcagg
     8881 gtactgcctg ggctgggctc cagttcccac ctcccttaaa ggaaaagaga gaaagaccca
     8941 gccaaataag agggttaagg ttatgggagg gggcatagac atccctcagg ctttggttgc
     9001 tggggactcc ttgctctgcc tgtctctctc ctaacccccc aattcctctg gtttcttccc
     9061 catctctccc tttccttgtt ccttcttccc ttcttcaggg tgggttaggc tggaggaaga
     9121 ggagggttaa ccacctctct ctcacccctc cccatgcctc ctaggggccc tgcaggccag
     9181 ggaagaggca ctctgggcac agctgggtga agcccatccg ggcaagcctt ccctggttac
     9241 tactgccttt ccccagagtc ctacagcagg cccctgcttc actttcaggc tcctgtctgc
     9301 ttctctaacc agatttctgt ttcccttcct gggctgctgg gaatctggtg agggagagtc
     9361 tgtgtcccgt gagcctcaca gagatgttca gtgcttggga tcgccgggaa cggcccccag
     9421 aggaaggagc agctgcaggg ctccagggct tcggagtgga caagaccttc ctgtcttccc
     9481 tcaaaggcat cctgctggaa actgagctgg taacagctgc cttccccaac ctcgtgcccc
     9541 cacctctccc atcccagccc cctggcatga ctctgggctg gcctcctgtg ctcaccattt
     9601 tccacccccc ttctcaggtt ttctgtcgtt ccactcacag gctttacctc ctgcaactcc
     9661 cagctttccg gccgcttctc tatctggagg cagtcttagc atgcatcatt caccgcaaga
     9721 cggaagttag atggaaagat agctttcgag gatactgagg gagtgagctg gggtgagggt
     9781 gggaagagga agaggaagca caggaagaag gccagtctgt ttccctccag cttctaggaa
     9841 ggctacttct tttttttttt tttttttttt tttttttttt ttttccattt tttattaggt
     9901 atttcgctca tttacatttg caatgctata ccaaaagtcc cccatagcca cccaccccct
     9961 ctcccctacc cacccactcc ccttttatgg ccctggcgtt cccctgtact ggggcatata
    10021 aagtttgcgt gtccaatggg ccggaaggct acttcttgac atgtctaagt ttaagtatcc
    10081 aagagggcag atctccggca gccacacttt atctgtcagt agtggcccat tgccatctag
    10141 tctcaggctt gccctggtta ctgagcaaga tcacagtgct cacccaggct gtcgttccta
    10201 tctgccggtc tataagttag caagatccaa ggcgcataac gataccacga tatcaacaag
    10261 tttgtacaaa aaagcaggct ggcgccggaa ccgaagttcc tattccgaag ttcctattct
    10321 ctagaaagta taggaacttc gaaccctttc ccacaccacc ctccacactt gccccaaaca
    10381 ctgccaacta tgtaggagga aggggttggg actaacagaa gaacccgttg tggggaagct
    10441 gttgggaggg tcactttatg ttcttgccca aggtcagttg ggtggcctgc ttctgatgag
    10501 gtggtcccaa ggtctggggt agaaggtgag agggacaggc caccaaggtc agcccccccc
    10561 ccctatccca taggagccag gtccctctcc tggacaggaa gactgaaggg gagatgccag
    10621 agactcagtg aagcctgggg taccctattg gagtccttca aggaaacaaa cttggcctca
    10681 ccaggcctca gccttggctc ctcctgggaa ctctactgcc cttgggatcc ccttgtagtt
    10741 gtgggttaca taggaagggg gacgggattc cccttgactg gctagcctac tcttttcttc
    10801 agtcttctcc atctcctctc acctgtctct cgaccctttc cctaggatag acttggaaaa
    10861 agataagggg agaaaacaaa tgcaaacgag gccagaaaga ttttggctgg gcattccttc
    10921 cgctagcttt tattgggatc ccctagtttg tgataggcct tttagctaca tctgccaatc
    10981 catctcattt tcacacacac acacaccact ttccttctgg tcagtgggca catgtccagc
    11041 ctcaagttta tatcaccacc cccaatgccc aacacttgta tggccttggg cgggtcatcc
    11101 ccccccccac ccccagtatc tgcaacctca agctagcttg ggtgcgttgg ttgtggataa
    11161 gtagctagac tccagcaacc agtaacctct gccctttctc ctccatgaca accaggctcc
    11221 caggtcccga aaaccaaaga agaagaacgc agatctctag agtcaccgag ttgctttacc
    11281 ggatgaagag ggccgaaaca tactgtccaa ggcccttgct ggcaatccac ccaactgaag
    11341 ccagacacaa acagaaaatt gtggcaccgg tgaaacagac tttgaatttt gaccttctca
    11401 agttggcggg agacgtcgag tccaaccccg gaccggtgca tgtggacccc aagaaaaagc
    11461 ggaaggtgga ccctaaaaag aaacgcaaag tggatcctaa gaaaaaaaga aaggtgcacg
    11521 tgtccatggt gtccaagggc gaggaactgt tcaccggcgt ggtgcccatc ctggtggaac
    11581 tggatggcga cgtgaacggc cacaagttca gcgtgtccgg cgagggcgaa ggcgacgcca
    11641 catacggaaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccttggc
    11701 ctaccctcgt gaccacactg acctacggcg tgcagtgctt cagcagatac cccgaccata
    11761 tgaagcagca cgacttcttc aagagcgcca tgcccgaggg ctacgtgcag gaaagaacca
    11821 tcttctttaa ggacgacggc aactacaaga ccagggccga agtgaagttc gagggcgaca
    11881 ccctcgtgaa cagaatcgag ctgaagggca tcgacttcaa agaggacggc aacatcctgg
    11941 gccacaagct ggagtacaac tacaacagcc acaacgtgta catcatggcc gacaagcaga
    12001 aaaacggcat caaagtgaac ttcaagatcc ggcacaacat cgaggacggc tccgtgcagc
    12061 tggccgacca ctaccagcag aacaccccta tcggcgacgg ccctgtgctg ctgcctgaca
    12121 accactacct gagcacccag agcgccctga gcaaggaccc caacgagaag agggaccaca
    12181 tggtgctgct ggaattcgtg accgccgctg gcatcaccct gggcatggac gagctgtaca
    12241 agcctgcagg cgagggcaga ggaagtcttc taacatgcgg tgacgtggag gagaatcccg
    12301 ggccgtccaa tttactgacc gtacaccaaa atttgcctgc attaccggtc gatgcaacga
    12361 gtgatgaggt tcgcaagaac ctgatggaca tgttcaggga tcgccaggcg ttttctgagc
    12421 atacctggaa aatgcttctg tccgtttgcc ggtcgtgggc ggcatggtgc aagttgaata
    12481 accggaaatg gtttcccgca gaacctgaag atgttcgcga ttatcttcta tatcttcagg
    12541 cgcgcggtct ggcagtaaaa actatccagc aacatttggg ccagctaaac atgcttcatc
    12601 gtcggtctgg gctgccacga ccaagtgaca gcaatgctgt ttcactggtt atgcggcgga
    12661 tccgaaaaga aaacgttgat gccggtgaac gtgcaaaaca ggctctagcg ttcgaacgca
    12721 ctgatttcga ccaggttcgt tcactcatgg aaaatagcga ccgctgccag gatataagga
    12781 atctggcatt tctggggatt gcttataaca ccctgttacg tatagccgaa attgccagga
    12841 tcagggttaa agatatctca cgtactgacg gtgggagaat gttaatccat attggcagaa
    12901 cgaaaacgct ggttagcacc gcaggtgtag agaaggcact tagcctgggg gtaactaaac
    12961 tggtcgagcg atggatttcc gtctctggtg tagctgatga tccgaataac tacctgtttt
    13021 gccgggtcag aaaaaatggt gttgccgcgc catctgccac cagccagcta tcaactcgcg
    13081 ccctggaagg gatttttgaa gcaactcatc gattgattta cggcgctaag gatgactctg
    13141 gtcagagata cctggcctgg tctggacaca gtgcccgtgt cggagccgcg cgagatatgg
    13201 cccgcgctgg agtttcaata ccggagatca tgcaagctgg tggctggacc aatgtaaata
    13261 ttgtcatgaa ctatatccgt aacctggata gtgaaacagg ggcaatggtg cgcctgctgg
    13321 aagatggcga tctcgagcca tctgctggag acatgagagc tgccaacctt tggccaagcc
    13381 cgctcatgat caaacgctct aagaagaaca gcctggcctt gtccctgacg gccgaccaga
    13441 tggtcagtgc cttgttggat gctgagcccc ccatactcta ttccgagtat gatcctacca
    13501 gacccttcag tgaagcttcg atgatgggct tactgaccaa cctggcagac agggagctgg
    13561 ttcacatgat caactgggcg aagagggtgc caggctttgt ggatttgacc ctccatgatc
    13621 aggtccacct tctagaatgt gcctggctag agatcctgat gattggtctc gtctggcgct
    13681 ccatggagca cccagtgaag ctactgtttg ctcctaactt gctcttggac aggaaccagg
    13741 gaaaatgtgt agagggcatg gtggagatct tcgacatgct gctggctaca tcatctcggt
    13801 tccgcatgat gaatctgcag ggagaggagt ttgtgtgcct caaatctatt attttgctta
    13861 attctggagt gtacacattt ctgtccagca ccctgaagtc tctggaagag aaggaccata
    13921 tccaccgagt cctggacaag atcacagaca ctttgatcca cctgatggcc aaggcaggcc
    13981 tgaccctgca gcagcagcac cagcggctgg cccagctcct cctcatcctc tcccacatca
    14041 ggcacatgag taacaaaggc atggagcatc tgtacagcat gaagtgcaag aacgtggtgc
    14101 ccctctatga cctgctgctg gaggcggcgg acgcccaccg cctacatgcg cccactagcc
    14161 gtggaggggc atccgtggag gagacggacc aaagccactt ggccactgcg ggctctactt
    14221 catcgcattc cttgcaaaag tattacatca cgggggaggc agagggtttc cctgccacag
    14281 cttgatgaag atctgagctc ttaattaatg atcataatca gccatatcac atctgtagag
    14341 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
    14401 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
    14461 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
    14521 ctcatcaatg tatcttatca tgtctggatc cgtttaaact aactttaaat aattggcatt
    14581 atttaaagtt acatatggcg gccgccctaa ggctcgacct cgaaattcta ccgggtaggg
    14641 gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg
    14701 cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg
    14761 gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc
    14821 ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact
    14881 agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc
    14941 ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc
    15001 gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ttccggaggc
    15061 ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct
    15121 ccgggccttt cgacctgcat ccatctagat ctcgagcagc tgaagcttac catgaccgag
    15181 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
    15241 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccaga tcgccacatc
    15301 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
    15361 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa
    15421 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
    15481 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
    15541 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
    15601 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
    15661 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
    15721 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc
    15781 cacgacccgc agcgcccgac cgaaaggagc gcacgacccc atgcatcgat gatatcagat
    15841 ccccgggatg cagaaattga tgatctatta aacaataaag atgtccacta aaatggaagt
    15901 ttttcctgtc atactttgtt aagaagggtg agaacagagt acctacattt tgaatggaag
    15961 gattggagct acgggggtgg gggtggggtg ggattagata aatgcntgct ctttactgaa
    16021 ggctctttac tattgcttta tgataatgtt tcatagttgg atatcataat ttaaacaagc
    16081 aaaaccaaat taagggccag ctcattcctc ccactcatga tctatagatc tatagatctc
    16141 tcgtgggatc attgtttttc tcttgattcc cactttgtgg ttctaagtac tgtggtttcc
    16201 aaatgtgtca gtttcatagc ctgaagaacg agatcagcag cctctgttcc acatacactt
    16261 cattctcagt attgttttgc caagttctaa ttccatcaga agctggtcga gtaactttaa
    16321 ataattggca ttatttaaag ttaggcgcgc cataacttcg tatagcatac attatacgaa
    16381 gttatgtcga gatatttaaa tattagaccc agctttcttg tacaaagtgg ttgatatctc
    16441 tatagtcgca gtaggcggtg ctcttggcat ctgactttta gccatcagga atagggaaag
    16501 ctttgtaggg cagaggtact gtgggctggt gtgtaccttg tgttctgtgt cccccgggga
    16561 cttccctggg gctctgcgga taccaaactg atggagttgc tgagattcct gggatagcag
    16621 gagtgggagg atggagatgc ccttgatggt ccctgacact agggatgagt cacttctgtg
    16681 tcttcactga gaactcttcc ttctcagtga ggctggggta gaagtaccag ctgagaagag
    16741 gagcccccta accacaggca aaccatggcc tggcctacgc atgtgtctcc tcagaaagct
    16801 ccccgtactt ggtcctgcag gccctgacct tcatcatctt catttgcttc acggcctcca
    16861 tctctgccta catggctgca gcgttgctag agttcctcat cacactggcc ttcctcttcc
    16921 tctgcgccac gcagtactac cagcgcttcg ataggctcaa ctggccttgt ctggtgagga
    16981 acgttgcctc tcccaccccc atcctggtct cttccttctc cttgtttgcc caattttcct
    17041 atagccctgt gactcccctt catttctaca actgagctgt gctttgactc gcgttgtgtc
    17101 ctgtgctcag acgtgaattt ttgacaccca tgagtgtgcc cttgtcaagg gggattgtca
    17161 aggagtatgt acagacctta gacctgtcct cccacacata aaaggcctag ctcagtcccg
    17221 ctccatgtgg tctgtttcct ggtctcccct ctgcctcctg gttgatctag atttatctgc
    17281 ttccatcctc tctgggtaag gttctgagct cacttccttg gaaatatttg acatgtccgg
    17341 aggcatcctt gggcaggaat tcagtctccg aggagcagac aggaaaatag gcaggaattc
    17401 agtctctgag ggccagatag ggaaagctga ctagtttgga agatctccag gtaatgatcc
    17461 cttccagcat cctccgaacc atcttccccc tgcctctggc tccccaggac ttcctccggt
    17521 gtctcagcgc catcgtcatc ttcctggtgg tctcctttgc tgctgtgacc tcgagggagg
    17581 gagctgccat tgctgctttt gtgagttcat tcctacagca ctctccagca cccaggactt
    17641 gaggctggca ttggcacatt tctggtgcct aaaacaaatc gtatgcccgc tcatacatca
    17701 ttctgtcact tctggtcccc attctccctc tcctagtctg atggcctctg ctgccccacc
    17761 cccatccctc tccataggtt tttggcatca tcctggtctc cgtctttgcc tatgatgcat
    17821 tcaagatcta ccgaactgag ctgatgccca gtaccactga gggtgagttg tctgtgccct
    17881 gagagaggag atgtccctac ctcttgtccc agataccaca ggcatacatg gtttccctgc
    17941 cttgctgcct ctccacaggg gaccagcagt gattccaggg ttacctggct cccacgacca
    18001 gacacaagga accgtggagc ccatctttgt catctttgaa ttcaccgaac cccaagtacc
    18061 caccatctat attccacgtt ttcagagatg tgcgcttgag aagtcccagg cacctgtggg
    18121 aactggtgaa gttctggagg ctggggcagg ttgttgctgg gcctccaagt tactcccaaa
    18181 ttaaaaaaaa aaaaatcaaa tcctagtggt gggtcttctt ccttatacag actaggaagc
    18241 caaagagtta ttcatgggag cgtgctttag agagccaaga tccctctgtg gggtgaggtg
    18301 gggcggctta gataggagat ggcgcaacgc aattaatgat aacttcgtat agcatacatt
    18361 atacgaagtt atggtctgag ctcgccatca gttcaacctc agcccgacac atcagaggaa
    18421 tgtgagatta ccaaaatgct atatgccagg ctgttcggga gtgagatgtc taggccttcc
    18481 tgatctacca agcaacagtc taacacacct gttagacacc ggctacagcc cttttctggg
    18541 gtttgtgtag agctccccct tgtggtatgg ggagggctgg gcagcagacc atagtggaga
    18601 ggagtgccaa aggtcttagg agaaattcct aaaataccag cacctgaaag aaaagtcctt
    18661 aggtgtgagc tggtcacgtg ccctcattct acgggaaaga aacctggggc ttaactgtgg
    18721 gggaagtggc ctcctggagg caaagctggc tccagaacat cttgaccttg gaacagtact
    18781 gtcttgatcc ttttttgagt ccttttgaag ctctgaaaac aaggggccca tccctttgcc
    18841 agtgtgcccc cttcctagct tgccacattc tcagaacacg tttgctgagt gtttcctgtc
    18901 tgcagagcct atgctcagga ccggagattt agaggtgacg gttgggcctg aaagggcaaa
    18961 tcgacttgga aaggagtttg agacagaaag ggcctggtcc ctgatagtct acctccccac
    19021 ctggctggag ggacccacca tggttcatct gtgtggctcc gttccagctg acttcttgcc
    19081 ccctctcttt aatgctttac tcttctgaaa tgcctttcct ccttccaatg agcctttctg
    19141 tcgcgggctc tggctgcgag ctctgactgt cccccagatc ttgaacttgg gggaatgtga
    19201 catgctttcc tgtgatgact atacttctct tagttgattt tcctgccttc tgtttcctct
    19261 cttcctatcg ttgtgttctg ttacaccatc gggcctggca gtcattccag ggaaggagcc
    19321 tactggactt tgggtttctc tggattctgt tcatggttct ccggcttcct gcatagtcta
    19381 tgcttgagtc tagatgattg actgcccttg gtcaggagga tggaggtagg gtgggcagag
    19441 tctcagagga gagatggaaa ggctgggctg gggaagtggg gagaagattg taggttttaa
    19501 agggtagaaa agggtctgga aaaccttgtc caaatgccca gaggacggca aggtttgctt
    19561 tccagccaga gatctcagct ccatccctga gcacctgtgt gtctagtcct ccaaggggag
    19621 agagacagag agagagagag acagagagag acagacagat agagacagag acagacagag
    19681 acagacatag agaaagacac agagagagag agagacagat atagagaaag acacacagag
    19741 acagagagac agaaacagag agagacagac atagagaaag atacagagat agagagagac
    19801 atacacagag acatagagag acagacagag acagacattg agaaagacac acagagaggc
    19861 agagagacag agagaaagac acagacagat agacagacat agagagacac acacagaaac
    19921 acacagagag gcacacagat agacagatag acagagagac agacacagag agacatagac
    19981 acacacacag acacacatac agacacacac atacacacac acacacacac acacacacag
    20041 agagagagag aggaaagaga gagttggact tgtgcctagc tgtgggtctg tccatgcacc
    20101 tatgtatgtc catgtgaagg atacaaaatg aagactgtcc ccatttggtc cataccccat
    20161 attgctttgg gcttggcatc atctggtccc tgagtcctgg aatgtcctca tggtgaggcc
    20221 agactcacag agctagggca gatgttgggg tgggaagatg taccctgtgg gctccatcta
    20281 aggagctggg tcgagtgtgg tctcagctgt ttcctgggtg tcccacagca gtttcctgtg
    20341 catccatccc cagcagtgca gagactcttt gggtaccagt ggaaagatca tcttggagag
    20401 aacacatggc ccggggcaac tttaatttct gccagctaag aggacgagct ggggaaggtt
    20461 ggcttccagc tcgtcagcaa acttccatcc ccctggatct gacttggctc gctttcagtg
    20521 cctctgttct ttggactcat tgaggggttg ttgttttctt gtctttgagg tgagctctca
    20581 cgctacccag gctggcttta aacttgcctt ctagctgagc ctgcctcttg cctccacctc
    20641 ccatctgagg cttggccatc cctcacacct ggatttgttt cccccacgtt tggaaagttt
    20701 ccctttctat acttgcacaa cgaccaagtg gagctggggc tgtagctgtg ctgcctgagg
    20761 caagacacct cacttctctc tcggcttcca cagaccatgg ggtgcttcag atcttacagc
    20821 tctgacagtc cctgctgtgg ccaccctcct cctggaccac caaaattccc accccagaga
    20881 gacgcttggt aggaaggaca gggtgcaggc agagtcgaac gtttatgttt attgtggatt
    20941 ggccacagcg agggtctgct ggagaggtta ttcctcgtcg tgcatcttct gctgagaaag
    21001 gagtaagaga tgtgaggtca gtgggtacga tggcctgggg aggaatttct agggatttca
    21061 gtggctctaa taaaatgccc ttgggaaggg aggggttgaa cctcagcagt cttgaagtct
    21121 catgatatca gtgcttgtag tttagaacac agatctgggg cccacttcaa gtgggagagt
    21181 ggactctcag gttacaggtg tctgtgggac ccttcggttg tatgtatgcc tattctatgt
    21241 ctaaatgcac atgggataca cacgtagctg ttcagagcat catgcttgca ggggcatcct
    21301 gatgagcgcg tgactagcag ttccaaggga ggaactcacc ttggcaccaa tgtcccggct
    21361 cttggcccgc agcttgttga cctgggactc ggcgatgtcc gccctctcct ccgcctcatc
    21421 cagctcgtgc tgcaccttgc ggaacttgga caggttggtg ttggcctgct cctcctatga
    21481 gccggtaaag ggggtaaagg ggaggggaga ctcgggtgag gagaactctg ggatgggctc
    21541 tcaggttctc ctggtccatg tccctcagcc actcctcact cagtttctgc aacagccttc
    21601 ccagggatgt cctgtttggc actctcctca aacgtgctca gtgttcaaga cccgtcatcg
    21661 ctacccactg cccggaatga acctgtgctg ccctgcgtgc tctgagaatc cacatccact
    21721 gcatctattc cagttgtgcc tcagacagat gcctgcagac ttgggggttt tctgcacatc
    21781 tgggctcatc cacaccacac ccacatgttc ctgtccaccc aagtgtcttc ttttaccctg
    21841 ggttcctatc caggctcaac gccaactctt ggccccttgc tgtcctgcat ctcaatgctc
    21901 ttccttagtc ttatggtata tggactgtaa ttaactagtg gacagatgta gggcaggaac
    21961 agcactgatc tttggacctt agggtgctag atacaattct gatcatactt gaagaataaa
    22021 tgtctgagct gatggttgga gcagactctg gtctcagccc cactgtggca aatgctcact
    22081 ggttctcact agcaggtgtg cggataaaag acggagacga aactctgaac tcaccgcctc
    22141 ctcagcctgg cgcttgtagg ccttcacctt caactgtagc ttgtccacca ggtcctgcag
    22201 ccgcattaag ttcttcttgt cttcctctgt ctgaaggtgg gtgggaggga tggaggagcc
    22261 agttttgggg agagaagcaa ttgtggttat cagtccctcc tcccttactc tattcctctc
    22321 gccaggcctc agaggcctca ccctccggtg gctcaggtca aggggtcgta ggacgaaagg
    22381 atttttcagt tagagaatca caaattaatg a
//