LOCUS       209632#pL1L2_GT2_LF2A_nEGFPO_T2A_CreERT_puro#L3L4_pD223_DTA_T_spec        22016 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT2_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 209632
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000016323
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            8861..8935
                     /db_xref="ENSEMBL:ENSMUSE00001309516"
                     /rank="4"
                     /note="ENSMUSE00001309516"
     exon            5405..5533
                     /db_xref="ENSEMBL:ENSMUSE00001256541"
                     /rank="3"
                     /note="ENSMUSE00001256541"
     misc_feature    4275..9762
                     /note="5 arm"
     misc_feature    9790..9814
                     /note="B1 Gateway"
     misc_feature    9827..9874
                     /note="Frt"
     misc_feature    9875..10374
                     /note="mouse En2 intron"
     primer          complement(9943..9964)
                     /note="LRPCR LAR3_EN2"
     primer          complement(9839..9862)
                     /note="FRT3"
     primer          14033..14051
                     /note="seq primer NF"
     misc_feature    14093..14100
                     /note="RE sites"
     misc_feature    15893..15926
                     /note="loxP"
     misc_feature    complement(14101..14132)
                     /note="Rox"
     misc_feature    complement(15853..15884)
                     /note="Rox"
     misc_feature    14133..14154
                     /note="polylinker"
     CDS             14713..15309
                     /note="puromycin N-acetyl transferase"
     misc_feature    14828..14833
                     /note="Mutated RsrII site"
     intron          10375..10749
                     /note="mouse En2 intron"
     misc_feature    10747..10749
                     /note="Splice Acceptor"
     exon            10750..10796
                     /note="mouse En2 exon"
     primer          10650..10671
                     /note="seq primer FCHK2"
     misc_feature    10797..10919
                     /note="1Dleader"
     misc_feature    10920..10971
                     /note="F2A"
     misc_feature    11067..11783
                     /note="EGFP"
     misc_feature    10977..11066
                     /note="3X NLS"
     misc_feature    13849..14087
                     /note="SV40 polyadenylation site"
     misc_feature    12882..13826
                     /note="ERT2"
     promoter        14165..14676
                     /note="PGK promoter"
     misc_feature    15420..15852
                     /note="PGK pA"
     primer          13907..13930
                     /note="LRPCR RAF5"
     misc_feature    11793..11846
                     /note="T2A"
     misc_feature    11843..11843
                     /note="A to G to kill RsRII site"
     misc_feature    11847..12872
                     /note="Cre"
     misc_feature    10765..10797
                     /note="FCHK lf2a_lacz_gt2"
     misc_feature    10765..10780
                     /note="FCHK QC prefix region"
     misc_feature    10781..10791
                     /note="FCHK QC critical region"
     misc_feature    10792..10797
                     /note="FCHK QC suffix region"
     misc_feature    15949..15969
                     /note="B2 Gateway"
     misc_feature    9763..15999
                     /note="Synthetic Cassette"
     exon            17348..17471
                     /db_xref="ENSEMBL:ENSMUSE00000161366"
                     /type="targeted"
                     /rank="6"
                     /note="target exon 1 ENSMUSE00000161366"
     exon            16369..16507
                     /db_xref="ENSEMBL:ENSMUSE00001256004"
                     /type="targeted"
                     /rank="5"
                     /note="target exon 2 ENSMUSE00001256004"
     misc_feature    16000..17796
                     /note="Critical Region"
     misc_feature    17820..17853
                     /note="Downstream LoxP"
     misc_feature    17797..17876
                     /note="synthetic loxP region"
     exon            21158..21236
                     /db_xref="ENSEMBL:ENSMUSE00000161362"
                     /rank="9"
                     /note="ENSMUSE00000161362"
     exon            20409..20521
                     /db_xref="ENSEMBL:ENSMUSE00000533198"
                     /rank="8"
                     /note="ENSMUSE00000533198"
     exon            19068..19143
                     /db_xref="ENSEMBL:ENSMUSE00000161367"
                     /rank="7"
                     /note="ENSMUSE00000161367"
     misc_feature    17877..22016
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggacgcaca agtgaatcag aaattatctg ctaagcagcc ctaaactaga
     4321 aagaggtgga gcaggaagcc caggctgaca agagctgatg ctgcgctgtc tcctggaaga
     4381 cattccagag gcagacatgc cttctctcct ggtgctccgg gatgcctgcc accgcaacac
     4441 cgtgcaaacc ttagagggac tcgggagctc tctgagagtt gtggctttag cttttaaagt
     4501 agtttagaca taaacttctc agtcatccct gtatccctta ggtccacacc tgcagattca
     4561 gccatcagaa agtcaaacat aacgaacggg agaaaacggt gtctgttctg gacacatata
     4621 ggtttgctcc tgtttggttc tccagtaaca cagcacagag gagtggtcgg cacagcattc
     4681 acctcaaaag cgaagatgct aacacagcca ccatcaagtt ccagctccca cccttagctt
     4741 agctgaggtt ccaatggcag atgcacaggg cccttcactc ctggagcagg ccaatcctcc
     4801 aggattctgt ccatcatgcc cccaaatcca cagagactgc aggaatggag gagagacggt
     4861 ctgccctgtg ttctggggtc agaatcagga tgatcaccag cagtgcgctg ataaaacaca
     4921 ctggtatttc cagctaccac agaaaccgag gcaagaggat tccatgaccc tagggatttg
     4981 tgagcagtct gaaagtagta agaccccacc aaattaagtt aaatgattca caaataaaag
     5041 ctaatccaac ggatccaggg gttttctttt ctcactgcat gtgagtctgt tctctcctgc
     5101 tactactgcc agccacatgg ccccacggct ctggcgagcc ttgctggggt tgtccatgtg
     5161 gagaaatgtc cagagccaat gcagagtctg tggggttgca tagggaaacg tgactgcctg
     5221 gtcccacgtc tgtcccgtgt gatcctcaag ggagagagca cactggtatg tcagataggc
     5281 agtgagcacc ccactcatct ggaaacttgt cagccataca ttcctcttct acacttcccc
     5341 ttggtttctg gcatgctctc ttccagccag agctacattc tctgtcttgg cctcttccct
     5401 acagaggagc tttctcagag gtgttcctgg tgaagcaaag agtgactggg aaactctttg
     5461 ctctgaaatg tatcaagaag tcaccagcct tccgggacag cagcctagag aatgagatcg
     5521 ctgtgttgaa aaggtgagtt ggcatcatgg ctgaaaggga gttggggtgg ggcaggtgta
     5581 gctgtgagat tcagacctgc ccttcccaaa ttacctttat ggcaaatgag cttgatttcc
     5641 acagctgtgg gtattgtgcc cttcgaattg ctgtgtcagg ttagtcagtt aagcccaggc
     5701 agctgtgcca gatgaaattt tttacccagc aaattgacta gttggactca ctggttcata
     5761 agtgtaggcc agagagtaat tggctcagag agtcaaacgc cagctgatgg ccatcagcag
     5821 gaagaatcaa tagctgagga ggcagcagga atcctgctgg cttccatcca tcctgcccca
     5881 gaattagcct tggttggatc caaagaaaac ccacagctgg gaaactgcag gaggctacac
     5941 ggatgcatca ccagtgttca cagtgagact cttacagtga gcctgtgaca tattaaaggg
     6001 tgacaggtga cccatgatca gagccatttg atgaggcaga cattgcccag gagcccacat
     6061 tgcccagctg aagagaagca tccataacta cgttgtctcc tatggtcttc atcttctgca
     6121 atccagcaga aagtcctgtg tggaagagac aggttcacgc ttgctggccc aggtgcctga
     6181 tgaatgtgga ttattagata atgttttcaa agaagaaaga ctttgatgaa tgagtggaat
     6241 tttgtgcaca aggaggcaga gcagaaagga aattcaagag tttttgcaaa cttttgtgca
     6301 gaatcatgga agtgtatgga gagagagtct atcctagaat caccgaagct ttgagtgggt
     6361 agcatcaagg acaggcagtg agaatagaat gttgagagga acaaggttgg cagagggtcc
     6421 taggatcaag gatgctggct ttattctatg aacaaagaga accaatggag ggtttggggt
     6481 aagaaaacag aatcagaata ataatcacct cccaccacca ccttacgtgt ggaagacggt
     6541 gggttaggag tcagcttaga gggtgggaga ctcattataa aaaccctcac agcaatttac
     6601 acagtgataa aaacatgaat ggcaggattt tgtgaagaca ggaagatcga acacagggcc
     6661 atctcaagga aaaaatgatc cagtttgagg ttagccaaca agagttcaga agagcagagc
     6721 atttaaagtg actctgagac taagccaagt ccctactaag tagaaagtga tgcagttgat
     6781 tagaataaag aacaaaaagc atagacttca aagaaacagg aagaaactaa ttggaacata
     6841 tggattgtag atgtctctgt gttgattatc cctacgtgga agagcctggc gggtacacta
     6901 tagtgacctt tctcactgca ctgtatcaac agcctagatt ttcagcgcac atcagttcat
     6961 tgcagggata atcagaagtt cgtgtggaca ttgccagcat gtgagggtgg tggagtccta
     7021 ggaaccaaca gtaccagctc tgggatttaa atatgcctta aatcataaag ctaatactat
     7081 ctatcccttc ccctgagcac tgtgccttgc attttcttaa aaaaaaaaag atttattttt
     7141 taacaatata tttactactc ttgtgtatgt gcattgtata tgatgagtgg atgcaggtgt
     7201 catggtaggc atgtggaggt ctgaaggcct tcacgtgaat ttctgggatt aaactcaagt
     7261 caagcgcctt aatcactgag ccatctgtgt ttttctcttt taatcccagt gcgaccacat
     7321 gagatcaatg cacttttcaa atcaaaacca accaaccaaa caacaaaagt gaggatttga
     7381 gaaagccaga taaagaaagc taggatattc cagagtcggg taaagtccta ggcccatcta
     7441 actcaatctc agcctcttgg aggcagtctt cctccccagg cctccacaga gtagctcctg
     7501 ggaaagccag acctctcact tgtcataatt cttctgctta actctagggc aggacagcag
     7561 gacaacccgt gggccagacc atcttatgtt gagaatggcc tgtacaccat gggctattta
     7621 gcaggctttc tacctctacc ttataggtga gggtggcagc cccagcctcc actaataaca
     7681 atcaaaatta tgtctctaga ggagtggaac tatggctcat cagttaggag tacatagtga
     7741 tgttaaaaag ggcccaggtt tagttcaaag ctcctctatc aggtgactcg caagcgccta
     7801 taactccagc tccagggaat ctaacaccct ctggcctctg tagtcatctg caggtatatg
     7861 gtgaacataa attcacacag gcaattatgc atgtgtgtgt gtgtgtgtgt gtgtgtgtgt
     7921 gtgtgtacac acacacacat acatgtgcac acacacacac aagtaaatac gtttttaaaa
     7981 gttgttagtt tttaaaaata tgttgctgac tatggtggta aatgaactta gcacttggga
     8041 gacagaggga ggagggtctg cacaagtttg aggtcagcct agtatacatg atgagttcca
     8101 gactagcctg ggtctgagct acatagtaac accctgctaa aagaaggaaa gggagagagg
     8161 gagggagggg cagaaggaaa gaccaaaaac cacaatgcaa agaataatta gaaagataaa
     8221 tagatgtttg gaagattgat ataagataaa aatagatgag ggatagataa gagacagata
     8281 aagatagata aattgattga ttgatagata gatagataga tagatagata gatgatacat
     8341 ggatgcattc acgtccttta aggggtaata tcattgatct aagacattcc acagaaaatc
     8401 agaatctacc tttttcatat ctcaacactc tttttgtggt tacttattgc ttttcctgtt
     8461 actatgacaa tcttaacacc tgaggaaaca aatttaaagg gagaggtttg ttctgactcc
     8521 cagcttgaag gatctagtcc atcatagaac agaactggta ggagcgtaag ccatctgggc
     8581 acactgcatc cacagtcagg aagccgagag gtgaatgcgg ttctcagtcc actgtcttct
     8641 ttattcagtg tggactccca gcccttgggg tggtactacc cacacacaga ggaggtctgt
     8701 catcctcagc tgcttcgcca cctcagcaag gcttctctga gacacggtcc tcttacacat
     8761 tgccatccaa cagtcactac aaacttttga gtgcgagtct gtacttttcg tctggatcac
     8821 ctgaaccctg tacccatcac aggattctta ccttatgcag gatcaagcat gagaacattg
     8881 tgaccctgga ggacatctat gagagcacca cccactacta cctggtcatg cagctgtaag
     8941 tgaagatgac cctcaccccc acagaggcct aggagtgggg ggcaggggaa aatgcctgcc
     9001 agataaagtc aagaactggc ttcagaggct ctgaaatgct atcctgtgga ctactgtgct
     9061 tccggtgcag ggctgagagg tcacactgag ccgactattg agttagttca tgccagcaca
     9121 ggctccaagt tgttggggta ctaactgtga acagacaata gctgttcaca ttcattgcct
     9181 cccataaggt cttctgggac ttcccaagca ctcagccact gaagaggaaa atgtcaggta
     9241 taatgtaaac ccccaaaacc tttctttggc acccttttaa tgtgtccatt tctatggact
     9301 tgatggtaaa catactaaca tcattgtaag ataaactgat tgtaagagta gaaatttcga
     9361 gtagatgatt tcaatgcggt ataaagaaca aatctctcaa gcgcgagctg cttgaattaa
     9421 atgcaggcta tgtgctgtat ccttctgtaa gacagaaatg gttcttcagt agagtggtct
     9481 ctgtcactgt catacctgct tcatgtggta ccaggggtgg atatgctacc actgcaagga
     9541 atcaggctta acatgatatc tagagattcg tgaagaatat actcaaggag aatatgatct
     9601 ttcatcagag attatgggtt ctctagcatg ctaatcttct agtactgatt ggaaactttt
     9661 agaaacatag catacatgat ttcacggata atgggaggtc aggctttatc tttaagctct
     9721 aaagtcctct ttccccatac cattcaagtt taaaatatgt ctaaggcgca taacgatacc
     9781 acgatatcaa caagtttgta caaaaaagca ggctggcgcc ggaaccgaag ttcctattcc
     9841 gaagttccta ttctctagaa agtataggaa cttcgaaccc tttcccacac caccctccac
     9901 acttgcccca aacactgcca actatgtagg aggaaggggt tgggactaac agaagaaccc
     9961 gttgtgggga agctgttggg agggtcactt tatgttcttg cccaaggtca gttgggtggc
    10021 ctgcttctga tgaggtggtc ccaaggtctg gggtagaagg tgagagggac aggccaccaa
    10081 ggtcagcccc ccccccctat cccataggag ccaggtccct ctcctggaca ggaagactga
    10141 aggggagatg ccagagactc agtgaagcct ggggtaccct attggagtcc ttcaaggaaa
    10201 caaacttggc ctcaccaggc ctcagccttg gctcctcctg ggaactctac tgcccttggg
    10261 atccccttgt agttgtgggt tacataggaa gggggacggg attccccttg actggctagc
    10321 ctactctttt cttcagtctt ctccatctcc tctcacctgt ctctcgaccc tttccctagg
    10381 atagacttgg aaaaagataa ggggagaaaa caaatgcaaa cgaggccaga aagattttgg
    10441 ctgggcattc cttccgctag cttttattgg gatcccctag tttgtgatag gccttttagc
    10501 tacatctgcc aatccatctc attttcacac acacacacac cactttcctt ctggtcagtg
    10561 ggcacatgtc cagcctcaag tttatatcac cacccccaat gcccaacact tgtatggcct
    10621 tgggcgggtc atcccccccc ccacccccag tatctgcaac ctcaagctag cttgggtgcg
    10681 ttggttgtgg ataagtagct agactccagc aaccagtaac ctctgccctt tctcctccat
    10741 gacaaccagg ctcccaggtc ccgaaaacca aagaagaaga acgcagatcg cagatctcta
    10801 gagtcaccga gttgctttac cggatgaaga gggccgaaac atactgtcca aggcccttgc
    10861 tggcaatcca cccaactgaa gccagacaca aacagaaaat tgtggcaccg gtgaaacaga
    10921 ctttgaattt tgaccttctc aagttggcgg gagacgtcga gtccaacccc ggaccggtgc
    10981 atgtggaccc caagaaaaag cggaaggtgg accctaaaaa gaaacgcaaa gtggatccta
    11041 agaaaaaaag aaaggtgcac gtgtccatgg tgtccaaggg cgaggaactg ttcaccggcg
    11101 tggtgcccat cctggtggaa ctggatggcg acgtgaacgg ccacaagttc agcgtgtccg
    11161 gcgagggcga aggcgacgcc acatacggaa agctgaccct gaagttcatc tgcaccaccg
    11221 gcaagctgcc cgtgccttgg cctaccctcg tgaccacact gacctacggc gtgcagtgct
    11281 tcagcagata ccccgaccat atgaagcagc acgacttctt caagagcgcc atgcccgagg
    11341 gctacgtgca ggaaagaacc atcttcttta aggacgacgg caactacaag accagggccg
    11401 aagtgaagtt cgagggcgac accctcgtga acagaatcga gctgaagggc atcgacttca
    11461 aagaggacgg caacatcctg ggccacaagc tggagtacaa ctacaacagc cacaacgtgt
    11521 acatcatggc cgacaagcag aaaaacggca tcaaagtgaa cttcaagatc cggcacaaca
    11581 tcgaggacgg ctccgtgcag ctggccgacc actaccagca gaacacccct atcggcgacg
    11641 gccctgtgct gctgcctgac aaccactacc tgagcaccca gagcgccctg agcaaggacc
    11701 ccaacgagaa gagggaccac atggtgctgc tggaattcgt gaccgccgct ggcatcaccc
    11761 tgggcatgga cgagctgtac aagcctgcag gcgagggcag aggaagtctt ctaacatgcg
    11821 gtgacgtgga ggagaatccc gggccgtcca atttactgac cgtacaccaa aatttgcctg
    11881 cattaccggt cgatgcaacg agtgatgagg ttcgcaagaa cctgatggac atgttcaggg
    11941 atcgccaggc gttttctgag catacctgga aaatgcttct gtccgtttgc cggtcgtggg
    12001 cggcatggtg caagttgaat aaccggaaat ggtttcccgc agaacctgaa gatgttcgcg
    12061 attatcttct atatcttcag gcgcgcggtc tggcagtaaa aactatccag caacatttgg
    12121 gccagctaaa catgcttcat cgtcggtctg ggctgccacg accaagtgac agcaatgctg
    12181 tttcactggt tatgcggcgg atccgaaaag aaaacgttga tgccggtgaa cgtgcaaaac
    12241 aggctctagc gttcgaacgc actgatttcg accaggttcg ttcactcatg gaaaatagcg
    12301 accgctgcca ggatataagg aatctggcat ttctggggat tgcttataac accctgttac
    12361 gtatagccga aattgccagg atcagggtta aagatatctc acgtactgac ggtgggagaa
    12421 tgttaatcca tattggcaga acgaaaacgc tggttagcac cgcaggtgta gagaaggcac
    12481 ttagcctggg ggtaactaaa ctggtcgagc gatggatttc cgtctctggt gtagctgatg
    12541 atccgaataa ctacctgttt tgccgggtca gaaaaaatgg tgttgccgcg ccatctgcca
    12601 ccagccagct atcaactcgc gccctggaag ggatttttga agcaactcat cgattgattt
    12661 acggcgctaa ggatgactct ggtcagagat acctggcctg gtctggacac agtgcccgtg
    12721 tcggagccgc gcgagatatg gcccgcgctg gagtttcaat accggagatc atgcaagctg
    12781 gtggctggac caatgtaaat attgtcatga actatatccg taacctggat agtgaaacag
    12841 gggcaatggt gcgcctgctg gaagatggcg atctcgagcc atctgctgga gacatgagag
    12901 ctgccaacct ttggccaagc ccgctcatga tcaaacgctc taagaagaac agcctggcct
    12961 tgtccctgac ggccgaccag atggtcagtg ccttgttgga tgctgagccc cccatactct
    13021 attccgagta tgatcctacc agacccttca gtgaagcttc gatgatgggc ttactgacca
    13081 acctggcaga cagggagctg gttcacatga tcaactgggc gaagagggtg ccaggctttg
    13141 tggatttgac cctccatgat caggtccacc ttctagaatg tgcctggcta gagatcctga
    13201 tgattggtct cgtctggcgc tccatggagc acccagtgaa gctactgttt gctcctaact
    13261 tgctcttgga caggaaccag ggaaaatgtg tagagggcat ggtggagatc ttcgacatgc
    13321 tgctggctac atcatctcgg ttccgcatga tgaatctgca gggagaggag tttgtgtgcc
    13381 tcaaatctat tattttgctt aattctggag tgtacacatt tctgtccagc accctgaagt
    13441 ctctggaaga gaaggaccat atccaccgag tcctggacaa gatcacagac actttgatcc
    13501 acctgatggc caaggcaggc ctgaccctgc agcagcagca ccagcggctg gcccagctcc
    13561 tcctcatcct ctcccacatc aggcacatga gtaacaaagg catggagcat ctgtacagca
    13621 tgaagtgcaa gaacgtggtg cccctctatg acctgctgct ggaggcggcg gacgcccacc
    13681 gcctacatgc gcccactagc cgtggagggg catccgtgga ggagacggac caaagccact
    13741 tggccactgc gggctctact tcatcgcatt ccttgcaaaa gtattacatc acgggggagg
    13801 cagagggttt ccctgccaca gcttgatgaa gatctgagct cttaattaat gatcataatc
    13861 agccatatca catctgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
    13921 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
    13981 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
    14041 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat ccgtttaaac
    14101 taactttaaa taattggcat tatttaaagt tacatatggc ggccgcccta aggctcgacc
    14161 tcgaaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta
    14221 gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca
    14281 tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact
    14341 cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa
    14401 atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag
    14461 cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag
    14521 aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc
    14581 gcccgaaggt cttccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg
    14641 ctgttctcct cttcctcatc tccgggcctt tcgacctgca tccatctaga tctcgagcag
    14701 ctgaagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
    14761 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
    14821 gtcgatccag atcgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
    14881 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
    14941 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
    15001 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
    15061 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
    15121 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
    15181 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
    15241 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
    15301 cccggtgcct gacgcccgcc ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc
    15361 catgcatcga tgatatcaga tccccgggat gcagaaattg atgatctatt aaacaataaa
    15421 gatgtccact aaaatggaag tttttcctgt catactttgt taagaagggt gagaacagag
    15481 tacctacatt ttgaatggaa ggattggagc tacgggggtg ggggtggggt gggattagat
    15541 aaatgcntgc tctttactga aggctcttta ctattgcttt atgataatgt ttcatagttg
    15601 gatatcataa tttaaacaag caaaaccaaa ttaagggcca gctcattcct cccactcatg
    15661 atctatagat ctatagatct ctcgtgggat cattgttttt ctcttgattc ccactttgtg
    15721 gttctaagta ctgtggtttc caaatgtgtc agtttcatag cctgaagaac gagatcagca
    15781 gcctctgttc cacatacact tcattctcag tattgttttg ccaagttcta attccatcag
    15841 aagctggtcg agtaacttta aataattggc attatttaaa gttaggcgcg ccataacttc
    15901 gtatagcata cattatacga agttatgtcg agatatttaa atattagacc cagctttctt
    15961 gtacaaagtg gttgatatct ctatagtcgc agtaggcgga ggtagctcag ttttccctaa
    16021 caaagaaaga agtcccattg taaatgcatg ccattaatct cattttcaca ctttgtccaa
    16081 tatccaatcc tgtgtccagg caggaaacaa aggtattcat aggaaggaat tcctagcttt
    16141 tccaggtgct ctacccaagg ctatttgaat tataatagaa gcttccagaa acaggccagc
    16201 caacacttga accaaatgac ccccagtgat agctcagtta cccagaagga gtgtcctgag
    16261 ccagcaatga atgctcgcac cccacttcag ggtgcctgcc catgcttaca gcccatggct
    16321 acagagaagt gcttggcctg ctatggaatc agattcttgt gcccacagtg tttctggagg
    16381 tgagcttttt gaccggatcc tagagcgtgg tgtctacaca gaaaaggatg ccagcctggt
    16441 catccagcag gtcttgtctg cggtgaaata ccttcatgag aatggcatcg tccacagaga
    16501 tctaaaggtg atggggcagg agtgctgggt ggggaacgca tgtcacatga gagtgttggc
    16561 atggttggac tcacaggctt tttctgccct aagattcctt atgctgagca tagacaacca
    16621 ttgtccaact gcacctgggc ctctgcaagg acctcctact tgtttagttt agtttagttt
    16681 tattttcttt tttatattta ttttttaatt ggatatttta tttgtttaca tttcaaatgt
    16741 tatcctcttt cccggtttcc cctctgcaaa ccccctattc catcttcctt taccctgctt
    16801 ctatgaggtt gctccccaac ctattcccgc ctcactgccc tagcattcct ctacactggg
    16861 gcttcaagcc ttcacaggag caaggacctc ccctcccatg gatgtcagat aaggcctgtt
    16921 cagctctttc agctcttccc ctgactcctc cattggggtc tcagggacct tttaaatgtt
    16981 ccctcattac aatgtcaaag ggatccacag ccacagtcaa attcagatgc taagggtcta
    17041 aaccacagtg ggcactactg cagtaggcat tgggggataa gagacaatta gccaaaggtt
    17101 gggtccctca aaatgcagtc tggagtagtt gggagtaggg aggagcaaag acaagaccat
    17161 aaacaagatg cagaattcta gggtacagaa gcaagggcac agatgggagg gaggtggaaa
    17221 aatagggaaa ggggctgtgg cctctgagtt gggagcccag ggcttccctt cctatgaaag
    17281 cccatatcaa ggacagcaca ctattcagga cgcccctttc tatgctgtat gtcttctcct
    17341 tcggcagcct gaaaacctgc tgtacctcac ccctgaggag aactccaaga tcatgatcac
    17401 tgactttggt ctatccaaga tggagcagaa tggagtcatg tccacagctt gtgggacccc
    17461 aggctacgtg ggtgagtcca ggggcagcag agtggagcca tttgtctgtt taaccactag
    17521 tcttgtagtt agctttctgg tgctgtgata acccaccatg accaaacgag gacaggccat
    17581 tatatcttat cttatagccc atcgtgaagg gaagtcaagg aaggatctca agaaggaagg
    17641 taaacacagg aactgaagca gaggccatgg agggatgctg ccactgactt acaccccatg
    17701 gcttgctcag cctgaattat accacccagg accaattgcc aagggatgga gccacacaca
    17761 ataggctggg tatcacatct cacatcaatc ctaagtgaga tggcgcaacg caattaatga
    17821 taacttcgta tagcatacat tatacgaagt tatggtctga gctcgccatc agttcacaat
    17881 ctgatcgaga caactcctga agtgaagtcc cctctcccta ataactctag cctgggacaa
    17941 actgaccagc agaacagtga tttgagagag aacatagaaa gatgactgtg tatagacata
    18001 ttcatctatc tgattatcta atcctgctct ttacagaggc tgccctcaac cattccaata
    18061 gattctaggc acactcttct accatcacaa ccctgagatt gagcagagag ctgaggggtg
    18121 gggcactcac cccatctttt cttcctacac tagaaccccc aactgctagg agtctggatc
    18181 cacatgatga tgagtgttgt cccaaggggt aactcctgga actccaggaa gcacaggaga
    18241 gtggtggagg ctgggcattg tttctactca gctgccagga ctcctgacca gagaagtacc
    18301 tgaccaggca cttgttgtta gcagacctgg gactcgagaa gaggaaagga aagagaatag
    18361 ttagccagcc atcacctgat gggctcggga aagtgggagg cagagcccag acttccagga
    18421 agaacctgct ctctcccaca ggaagtttag tcactcacct aaagatgtgg aattctgtgg
    18481 atactaaacc cagtactggg gtacattcag aaggcaggtc tctgcctggc tttcccaatc
    18541 caaaggaagc atggcttccc ttactgagcc ctcaagttca tgcactaggc aagtgccaga
    18601 gatgagcaac tcccagtcac tttctgggag cttcctgaga tgactagagt aggaagggag
    18661 cctttgcctt ccagggcttc tgctgctgtg gggaagagac catgctaccc agcagacccc
    18721 tgctttgaat acagagacga cccttgccct gaggggcctc cagatgagca gagtcaggtc
    18781 ttactcttag agagtagtcc attggaaata gcaaagctgc agacttctgg gcttatgagg
    18841 agttagaaac agcttatggg aattcccaga ttgatgaagg aacaggtctc tcctctaagg
    18901 aagtctgctt ggggagatac atctctacct ttggggagct ttcagtctca tggatgttcc
    18961 ctactctacc cttctgtccc ctacttccat ccctttcctg ttctctactc ctctctcggg
    19021 cctcctttct tgtccctagc cctgaaccta cgattgattt tctacagctc cagaagtgct
    19081 ggcccagaag ccctacagta aggctgtgga ctgctggtcc attggtgtca tcacatacat
    19141 actgtgagta aaagctggta tcagtgcatt ccctgagaaa tcacatacac acacacacac
    19201 acacacacac acacacacac acacacacac acggcagggg agggaagaga gggaaagggg
    19261 gagaggggca gggagaggag gcggggaaga gggagagaga ggttcactta tgtgcatagg
    19321 tgtcactgaa ctccatcaag ccatggacag aacccagcct gtgctcattc ttcaggccca
    19381 tggattaccc ttctcctggg cactgcattc ccaccctctg tacaaacgtg aggccagcca
    19441 ctctgtagaa ccatcaagag agtgagagat caaggcctag aacccatgta ggccgaagta
    19501 tctcagctcc tccaacctag cagtgacacc atctgaaggc agcatcgtaa actaaagatt
    19561 ctcagcattt tctctctgcc acacatgctc aggtgctcca tttcctagcc ttcccaaggg
    19621 ttctccccca atatgtgttc ccacccccat agcagccagc tttgttctta gggaagtaac
    19681 agttgccgct cagtgtggat tctgttaaat tattctctaa ctcatttact cagataacat
    19741 ctgagtcatc tcctaggcta gggccaaggg atgtagctca gttggcatgg cgcctgccta
    19801 gaatgcatga aaccctgtgt tcaatcctag cacagtctac aactgagtgg taggaagggg
    19861 gctagagaaa taactcagca gttaagaaca cttactgatc ttccagaaga cccacatcag
    19921 gcctgtaatt ccagctccag gctgatctga cacacagaca cacattaaca ttaaaattaa
    19981 actaaagcat cttttttttt tttttaaata acctgggtat ggtagcacaa tgtacaatcc
    20041 taagaggaga cagaggggag gagccatcct tagctatgca gcaagtttga agttaacttg
    20101 agctacatga gactctgtct caaagagtaa accaaaacag tttaatcccc tgcagtagcg
    20161 ttcctcgttc ttcataagaa tcctttgtag agatcaccca agagccagag gaagtgaggg
    20221 cccagtgggc agatgtcctg caaaggatcc tctcaaagtc tcctctccag gcctcagctc
    20281 cgcatgtgct cttgtagtca cactgccggt ctgagacctt cactgactgt gaattgtgca
    20341 gtgggaggct agatagagag ggtggtccag ggaggaggga actcctcaca gactatgtct
    20401 tcctgcaggc tgtgtggcta tccccctttc tatgaagaaa cagaatcaaa gctttttgag
    20461 aagatcaaag agggttacta cgagtttgag tctccgttct gggatgacat ttctgagtca
    20521 ggtgagcatg aaggacaaag cagaggctgt aagagaaagt atcttaatgg agaagacagt
    20581 ccctggggct catgcggcag aagctgccag ggagagcaga gaagtctctc cccagttcaa
    20641 aggccaggtg aagtacttga ggctaggacc cagagaggag ggttacaggc ggggggcaca
    20701 acgtgaggtc aggtaacaga acagaactga ccctgatgtg gaagaaggtg gcatacacac
    20761 tatgtgagag agagaaggca gcatgcattc tgtattggaa aaaaatgagc caggtctgct
    20821 tgtggagcct ggtaagtggt ggagagagct ggaaggatcc aacactcaca tgctaaccca
    20881 tgtgcttacc agcaaatgta tgcacacggg gtgcaggcac attggcatga cgtaactggc
    20941 aaggctcccc tgtacatgtg ggtgaggctg cctagagctc tcttcttggt ggcttatggt
    21001 gggttgttag gtagtctgga ttttttttta agactcttaa aactgtctct ttagaagatt
    21061 aactaaagat gcactcactg cctcccacca gcccagaagt gaatttcttt tagtcgtgaa
    21121 atctggtcct tagctgaatc tctgatattc cccttagcca aggattttat ttgccatctg
    21181 ctggagaagg acccaaacga acggtacacc tgcgagaaag ccctcagaca cccctggtaa
    21241 gtaagaaatg gggtaggctg gagccagctg ccctgggacg tcaaagccag tgatgttcat
    21301 gaggcccagc tggccagcac acagcactga gttgggccag tgtggcctgc aggggccagc
    21361 tacgtgctct aaccacttct aaaagcaaag gccaagggtt ctatcttcaa gctggatgac
    21421 taaagaggca acctgtcctg aggggcatgt tgctggagat gaagcttggt ggctgagtgc
    21481 ctagcgtgtt tagccatcca ggaggcttaa gtcagtgcct agttccaaag atagtggtgt
    21541 gatagtggtg tatggtacac acacacacac acacacacac acacacacac acacacacag
    21601 agtcacgtga caagtaaact tacaagatgg taaacagact cttgaagcac tacttcctgt
    21661 cgcctgctca ggatcttaag agttgtgccc accttccccc atagcaagca cagaagcctc
    21721 acctctccag gctttccctg acctgatcca gtcttgtttt ttcctcagtg cccacagttc
    21781 tagccaaata tctctgattc agactcacga gctagccctc tcctcctgcc tccctctccc
    21841 ctcaccccaa gctttttctt taccacacct ttggtcacca cagaaatata ttcttcctct
    21901 tgacttacat gtccagatca ctgccctgag ttcagccctg acttcttagt ccccacagat
    21961 cttccattac acagattgcc agatcttcca tttgtccttt taattacaca gcttgc
//