LOCUS       213242#pL1L2_GT0_LF2A_nEGFPO_T2A_CreERT_puro#L3L4_pD223_DTA_T_spec        19586 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT0_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 213242
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000027959
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            6458..6618
                     /db_xref="ENSEMBL:ENSMUSE00000495823"
                     /rank="1"
                     /note="ENSMUSE00000495823"
     exon            7843..7990
                     /db_xref="ENSEMBL:ENSMUSE00000494744"
                     /rank="2"
                     /note="ENSMUSE00000494744"
     misc_feature    4275..9789
                     /note="5 arm"
     misc_feature    9817..9841
                     /note="B1 Gateway"
     misc_feature    9854..9901
                     /note="Frt"
     misc_feature    9902..10401
                     /note="mouse En2 intron"
     primer          complement(9970..9991)
                     /note="LRPCR LAR3_EN2"
     primer          complement(9866..9889)
                     /note="FRT3"
     primer          14053..14071
                     /note="seq primer NF"
     misc_feature    14113..14120
                     /note="RE sites"
     misc_feature    15913..15946
                     /note="loxP"
     misc_feature    complement(14121..14152)
                     /note="Rox"
     misc_feature    complement(15873..15904)
                     /note="Rox"
     misc_feature    14153..14174
                     /note="polylinker"
     CDS             14733..15329
                     /note="puromycin N-acetyl transferase"
     misc_feature    14848..14853
                     /note="Mutated RsrII site"
     intron          10402..10776
                     /note="mouse En2 intron"
     misc_feature    10774..10776
                     /note="Splice Acceptor"
     exon            10777..10816
                     /note="mouse En2 exon"
     primer          10677..10698
                     /note="seq primer FCHK2"
     misc_feature    10817..10939
                     /note="1Dleader"
     misc_feature    10940..10991
                     /note="F2A"
     misc_feature    11087..11803
                     /note="EGFP"
     misc_feature    10997..11086
                     /note="3X NLS"
     misc_feature    13869..14107
                     /note="SV40 polyadenylation site"
     misc_feature    12902..13846
                     /note="ERT2"
     promoter        14185..14696
                     /note="PGK promoter"
     misc_feature    15440..15872
                     /note="PGK pA"
     primer          13927..13950
                     /note="LRPCR RAF5"
     misc_feature    11813..11866
                     /note="T2A"
     misc_feature    11863..11863
                     /note=" A to G to kill RsRII site"
     misc_feature    11867..12892
                     /note="Cre"
     misc_feature    10792..10817
                     /note="FCHK lf2a_lacz_gt0"
     misc_feature    10792..10807
                     /note="FCHK QC prefix region"
     misc_feature    10808..10811
                     /note="FCHK QC critical region"
     misc_feature    10812..10817
                     /note="FCHK QC suffix region"
     misc_feature    15969..15989
                     /note="B2 Gateway"
     misc_feature    9790..16019
                     /note="Synthetic Cassette"
     misc_feature    16020..19586
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggatttccc aaatctgact ctttgattcc acaaccttat ttcctaatgt
     4321 atgctacaga catgcccaca tgccaagtga cgcatgcgca gaggcattgc tgccgtgtct
     4381 tcgggagaag tttctgtgtt cagcacgcag agcagatgag atgcgcacct tcagtggcgc
     4441 acacgtggtt attaaaagaa gtcctctaca ctagcgcatg ctatagcaca catacgtcat
     4501 acatggtttc ttcacggttt tgttttgaga cagaatcttt ctctgtagtc ggttcacagg
     4561 aactctgttg tcttgagggc cgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg
     4621 tgtgtgtgtg tgtgtgtgtt gtatgcttcc caaatcctca atgttacagc tctcattatt
     4681 tggggataat aaaggttata taaatcttgg agagtaggta gggcttttga ggtgagtgta
     4741 cattattggc tagggctgag gtgctgggtg ctggggatgc ttcatttaca tcaagaggaa
     4801 atccaggtgc ttgctggaca tgttgagaga gagagagagg gagtgagaga gagggagtga
     4861 gagagaggga gtgagagaga gggagaggga gagggagagg gagagggaga gggaggagag
     4921 agagggggaa gagagggaga gggagaggga gagggagagg gagagggaga gggagaggga
     4981 gagggagagg gagagggaga gggagaggga gagggagagg gagagggaga gggagaggga
     5041 gagggagagg gagagggaga gggagaggga gaggatgaag ctgagccatc tctccagccc
     5101 aggtgggcac tatcttgcac atctgtctag gtagaggagt gtgtggctta caggtacagt
     5161 tggctttgtc acgtgggttc tgggacaata ctcaggccat caggcttctc agggaagtag
     5221 ttttttaacc cactgagcca tcttgctgat cacctctgga ttctcttttt gttgttgttt
     5281 tgagacacat taagactgtg tagccacagc tgaccgggaa ctcactatgt agagcaggct
     5341 gacctcaaac tcagagatcc accagctctg gctcccaatt tttgggatga aaagtgtgca
     5401 cctcacattc acctcccatc cccaccccag gcactcttaa gccagtaaaa ttcttgagcc
     5461 ctctttgcat gaagtgccta caggccatgt tagcacagtt aatgctctga agtaggacag
     5521 tctacatttc tcatgcaagg tcgctaaagc ccgggctgat gaacttttct aaggcctcat
     5581 tttccaagca cagtcagaat ttcacccaga taatttggct ccaggatgga cattaatggt
     5641 tgtcctcact taggtctgag ttcacacact gtccacagag ctgaggaaaa actgccttga
     5701 tagttaacga agacagcagt tcccagagaa gccatggcta cagagctgtc actttgcctc
     5761 ttgtgggcaa aatggatgtt cgagagcaga gacagccctt gatcctaaaa taactcctgt
     5821 gtcctccatc actctagaga gtaatgagaa ccacagggac agagcacacg gggctgctgg
     5881 cctatgtcac attacccagc tgtgaactgt cagagcctgt aataagagct gttcttaacc
     5941 cattatctgg aagggtcagg agtatcatta tgctgggttg gataggagaa acattttagg
     6001 ggaaaaaaga actagcaatt atctccccac tgaattttgg aggcagcctc aagtaaccca
     6061 aacttggagc catcctcctg cttcagcatc ccaaaggctt ggattgcagg tataaacaac
     6121 cacatgcagc ttgctttctt gaaagaaaca atttgttttt aaatcatatg tgtgagttga
     6181 ggtagctgtt tcagaagagg gcgtcagatt ccacatagct gtggttacag aggctgagag
     6241 ccatgggcac tgggaaagcc agcttggctc tactggatga gcagagagca ctcccagtca
     6301 ctgagccatc tccccagatc ccttccccac cccacgtcct tccttcaaac cagcacctat
     6361 gatctataga ataatgggtt tcattgtgac attttcattc atgtaggatt ctgattgcgt
     6421 tcaccctctt atgcccctcc ccctcccagt ggaccccacc tgcttccccc tataccctct
     6481 tctcacttca cgtggacttt tgttcttgtt gtttggttgt tgagataagg cctttctatg
     6541 catccctgac tggcctggaa cttactatgt aaacctggtt ggctcacatt tagaggtctg
     6601 actcctccaa gttctgaggt tagaggcgtc accatgcttt cgtgtttgag tgtgtgtgtg
     6661 tgtgtgtgtg tgtgtgtgtg tgtgtgtgtc tgagctcaga gatgcttgca cacgtgggag
     6721 gttattccca ggagcatgga ccacctacca gtgtggctgg ttacaacact gaaaagaaga
     6781 tcctgccctc tccagaacta ttaacataaa gggcggggcc ttgtgagccc ctcccccagc
     6841 aacagttaac gcctccatag ctccaggaag gagggggggc ctcatgagtc ccttccctgt
     6901 tttttggaga caggatccta ctacatagcc aaggctggtc ttgaacttga gatcctcctg
     6961 cctccacttc ctggatactg ggattacagg cgtgcacagc catatctggc tcttaagtat
     7021 ctttggacaa cttgggtttt ttcctttttt taaaacttaa gactttgtaa ctgacaagcc
     7081 ctctttagtt ctaacacccc aatagatgag tagatggact taacagaaat ttcaatgagt
     7141 ctctcagggt tccttggttt tatagctaat aggctcaggc cctggtacct actcagtttc
     7201 ccaccctacg ttctgatttg ccatatccta accaacacta tgaagctata tcttgggtcc
     7261 tgtgactgta gggggatgga ggagacagga gtcacttaag gaccaaggat aagacatata
     7321 tttccttttg ggaacaaacc agttggttgc ttcctaagca gtgtgccttt gttatcctgt
     7381 gagcagcatc ttggcagcgc ccaattccca ctgtgaacat ttcattcacc ttattcttca
     7441 gtgtacaaag caggccatca ctacagtgtg tgatggatga ggggttaggc ttagttcatt
     7501 ataccacagt gtctcacaca ctcagctcac gggggttggg gggtctcaaa gtttgtttaa
     7561 atagttgaag atgtcattca aagacaacct ggaaattctg aagcagactt aactatgttg
     7621 gggaaacagc tatttacatg acagttatcc ctgatttgtt ttggacacat gcaaagtata
     7681 gtaactgagg caaatgcagc ggtcctgctc agtggtcctg ggattttgga agcagaggca
     7741 ggaggacggg gtgttcaagg tatccatggc tacttctcag ttcattttct catgtttgtt
     7801 tgggcctcta tactctaaaa tgaaaatgaa cctacgtttc agagagaaag gaagagggta
     7861 gtgtaaacca cagaatgtag cccaacccct gctgaccggg agtaactccc agaaggctgg
     7921 ccttctgcag ttgccaggca acaagtgtaa acagcactgg atcatggcca ctaactacag
     7981 tgccaaccag gtaagcgctt gctcgtcacc cttttacatt tgagtttatg ttccagatgc
     8041 tgcccttgac tgcccgctcc catcagactc tgactgggtt gaggcggtgc ttcatctcat
     8101 gggtgggagg gcccaggaac aagcaaagag gatctgggtt aaatctgtag accgtaagcc
     8161 aggaagcagt gattttattt gtacaggcca gagtaagcag tttaggcttt gtaagtcaag
     8221 tgacaaagtc atatctattt atatgaaagt tagcactaat ttgtttcaga aaaattcaaa
     8281 atataggggt agagatgctc cccagtggtc cccgaggcag gaggatgggt gttcaaggca
     8341 ttctggggct acatactgag tccaaggcta acctgagctt caagagaacc cctccccctc
     8401 cacacacaca cagagagaga gagaaagaga gagagagaga gagagagaga gagagagaga
     8461 gagagagaga gagagagaga gagaatgaaa acaaggacac aaaatagtca ctgatggttc
     8521 gaggccagcc tggtctacat agagagttcc aggccagcca gagtcttgta gtaagaaaag
     8581 aaacaaaata ttgcctacac atttttggag ttcctttgga gaaaatattt tccttcttta
     8641 gtattcccag tttagtgttg ttctttttct tcgtttatgt gtatgtgtgt gagtaggtag
     8701 acaggtaagg ttttgctact acctacctat cccagactgg accacaaagt cacaaccctc
     8761 ctaccttcct ctgctaagcg tgaggcttac aggtgtgtgc caccttggcc aactaccttc
     8821 tgtcaaagca acacaaatgg ttgttttatg tctacaaata tttttacaaa tggtctattt
     8881 ttacaaataa aaaacgttct tgccagggag gcagggacag aagggtggaa cccagcctca
     8941 aagaaagaaa gaaaaagaga gagagagaga gagagagaga gagagagaga gagagagaga
     9001 gagagagaga gagagagaga gagagaggaa agaaagaaag aaagaaagaa agaaagtggc
     9061 gttttctgcc ccttggcctt ctaaaagcag gtagcaatgt ggatttggtt accatccctt
     9121 ggtctaatct agcagtctcc agcagtgtgt cacaaacata atttaaaagg ttttagcagc
     9181 tatgtgaaaa ttaaaaatta aattttgtaa tgctcagtag aatataataa acctcaaaca
     9241 ttaacattta attgcatttt taaaaagtct aaccacacac acacacacac acctatttta
     9301 taccagctcc ttaaaatcca tttttcccca ctagcgctga ggaccatagc tctgtacatg
     9361 ctagataagc actgtaactc agagctacac ccctggtaca catcttgagc aaacagatta
     9421 taatctcata cttacctagc acgcacaaga tcctgggttc aatatgcagc gtggcaaagc
     9481 cagacacaat ctcgtctctc tttgtattta gctctcttct tttcgtgttg cttgtaggtt
     9541 ggcataccaa ctgttgggtt ttgtcagggc atcttcctac acaagagtca gtatactgtt
     9601 ctcaaccctt ccctccagct ggtaggcttt tcttcccaga cttagtgccc aggtcagctc
     9661 tccttatact ctgcaaccca ttgtcttagc acctacctcc cttatgatag cttcctccct
     9721 acataccctt gccacagtga gttcgagttc acctaactgt agagaacagt gtcaccatct
     9781 cacgggggaa aggcgcataa cgataccacg atatcaacaa gtttgtacaa aaaagcaggc
     9841 tggcgccgga accgaagttc ctattccgaa gttcctattc tctagaaagt ataggaactt
     9901 cgaacccttt cccacaccac cctccacact tgccccaaac actgccaact atgtaggagg
     9961 aaggggttgg gactaacaga agaacccgtt gtggggaagc tgttgggagg gtcactttat
    10021 gttcttgccc aaggtcagtt gggtggcctg cttctgatga ggtggtccca aggtctgggg
    10081 tagaaggtga gagggacagg ccaccaaggt cagccccccc cccctatccc ataggagcca
    10141 ggtccctctc ctggacagga agactgaagg ggagatgcca gagactcagt gaagcctggg
    10201 gtaccctatt ggagtccttc aaggaaacaa acttggcctc accaggcctc agccttggct
    10261 cctcctggga actctactgc ccttgggatc cccttgtagt tgtgggttac ataggaaggg
    10321 ggacgggatt ccccttgact ggctagccta ctcttttctt cagtcttctc catctcctct
    10381 cacctgtctc tcgacccttt ccctaggata gacttggaaa aagataaggg gagaaaacaa
    10441 atgcaaacga ggccagaaag attttggctg ggcattcctt ccgctagctt ttattgggat
    10501 cccctagttt gtgataggcc ttttagctac atctgccaat ccatctcatt ttcacacaca
    10561 cacacaccac tttccttctg gtcagtgggc acatgtccag cctcaagttt atatcaccac
    10621 ccccaatgcc caacacttgt atggccttgg gcgggtcatc ccccccccca cccccagtat
    10681 ctgcaacctc aagctagctt gggtgcgttg gttgtggata agtagctaga ctccagcaac
    10741 cagtaacctc tgccctttct cctccatgac aaccaggctc ccaggtcccg aaaaccaaag
    10801 aagaagaacg cagatctcta gagtcaccga gttgctttac cggatgaaga gggccgaaac
    10861 atactgtcca aggcccttgc tggcaatcca cccaactgaa gccagacaca aacagaaaat
    10921 tgtggcaccg gtgaaacaga ctttgaattt tgaccttctc aagttggcgg gagacgtcga
    10981 gtccaacccc ggaccggtgc atgtggaccc caagaaaaag cggaaggtgg accctaaaaa
    11041 gaaacgcaaa gtggatccta agaaaaaaag aaaggtgcac gtgtccatgg tgtccaaggg
    11101 cgaggaactg ttcaccggcg tggtgcccat cctggtggaa ctggatggcg acgtgaacgg
    11161 ccacaagttc agcgtgtccg gcgagggcga aggcgacgcc acatacggaa agctgaccct
    11221 gaagttcatc tgcaccaccg gcaagctgcc cgtgccttgg cctaccctcg tgaccacact
    11281 gacctacggc gtgcagtgct tcagcagata ccccgaccat atgaagcagc acgacttctt
    11341 caagagcgcc atgcccgagg gctacgtgca ggaaagaacc atcttcttta aggacgacgg
    11401 caactacaag accagggccg aagtgaagtt cgagggcgac accctcgtga acagaatcga
    11461 gctgaagggc atcgacttca aagaggacgg caacatcctg ggccacaagc tggagtacaa
    11521 ctacaacagc cacaacgtgt acatcatggc cgacaagcag aaaaacggca tcaaagtgaa
    11581 cttcaagatc cggcacaaca tcgaggacgg ctccgtgcag ctggccgacc actaccagca
    11641 gaacacccct atcggcgacg gccctgtgct gctgcctgac aaccactacc tgagcaccca
    11701 gagcgccctg agcaaggacc ccaacgagaa gagggaccac atggtgctgc tggaattcgt
    11761 gaccgccgct ggcatcaccc tgggcatgga cgagctgtac aagcctgcag gcgagggcag
    11821 aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc gggccgtcca atttactgac
    11881 cgtacaccaa aatttgcctg cattaccggt cgatgcaacg agtgatgagg ttcgcaagaa
    11941 cctgatggac atgttcaggg atcgccaggc gttttctgag catacctgga aaatgcttct
    12001 gtccgtttgc cggtcgtggg cggcatggtg caagttgaat aaccggaaat ggtttcccgc
    12061 agaacctgaa gatgttcgcg attatcttct atatcttcag gcgcgcggtc tggcagtaaa
    12121 aactatccag caacatttgg gccagctaaa catgcttcat cgtcggtctg ggctgccacg
    12181 accaagtgac agcaatgctg tttcactggt tatgcggcgg atccgaaaag aaaacgttga
    12241 tgccggtgaa cgtgcaaaac aggctctagc gttcgaacgc actgatttcg accaggttcg
    12301 ttcactcatg gaaaatagcg accgctgcca ggatataagg aatctggcat ttctggggat
    12361 tgcttataac accctgttac gtatagccga aattgccagg atcagggtta aagatatctc
    12421 acgtactgac ggtgggagaa tgttaatcca tattggcaga acgaaaacgc tggttagcac
    12481 cgcaggtgta gagaaggcac ttagcctggg ggtaactaaa ctggtcgagc gatggatttc
    12541 cgtctctggt gtagctgatg atccgaataa ctacctgttt tgccgggtca gaaaaaatgg
    12601 tgttgccgcg ccatctgcca ccagccagct atcaactcgc gccctggaag ggatttttga
    12661 agcaactcat cgattgattt acggcgctaa ggatgactct ggtcagagat acctggcctg
    12721 gtctggacac agtgcccgtg tcggagccgc gcgagatatg gcccgcgctg gagtttcaat
    12781 accggagatc atgcaagctg gtggctggac caatgtaaat attgtcatga actatatccg
    12841 taacctggat agtgaaacag gggcaatggt gcgcctgctg gaagatggcg atctcgagcc
    12901 atctgctgga gacatgagag ctgccaacct ttggccaagc ccgctcatga tcaaacgctc
    12961 taagaagaac agcctggcct tgtccctgac ggccgaccag atggtcagtg ccttgttgga
    13021 tgctgagccc cccatactct attccgagta tgatcctacc agacccttca gtgaagcttc
    13081 gatgatgggc ttactgacca acctggcaga cagggagctg gttcacatga tcaactgggc
    13141 gaagagggtg ccaggctttg tggatttgac cctccatgat caggtccacc ttctagaatg
    13201 tgcctggcta gagatcctga tgattggtct cgtctggcgc tccatggagc acccagtgaa
    13261 gctactgttt gctcctaact tgctcttgga caggaaccag ggaaaatgtg tagagggcat
    13321 ggtggagatc ttcgacatgc tgctggctac atcatctcgg ttccgcatga tgaatctgca
    13381 gggagaggag tttgtgtgcc tcaaatctat tattttgctt aattctggag tgtacacatt
    13441 tctgtccagc accctgaagt ctctggaaga gaaggaccat atccaccgag tcctggacaa
    13501 gatcacagac actttgatcc acctgatggc caaggcaggc ctgaccctgc agcagcagca
    13561 ccagcggctg gcccagctcc tcctcatcct ctcccacatc aggcacatga gtaacaaagg
    13621 catggagcat ctgtacagca tgaagtgcaa gaacgtggtg cccctctatg acctgctgct
    13681 ggaggcggcg gacgcccacc gcctacatgc gcccactagc cgtggagggg catccgtgga
    13741 ggagacggac caaagccact tggccactgc gggctctact tcatcgcatt ccttgcaaaa
    13801 gtattacatc acgggggagg cagagggttt ccctgccaca gcttgatgaa gatctgagct
    13861 cttaattaat gatcataatc agccatatca catctgtaga ggttttactt gctttaaaaa
    13921 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
    13981 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
    14041 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
    14101 atgtctggat ccgtttaaac taactttaaa taattggcat tatttaaagt tacatatggc
    14161 ggccgcccta aggctcgacc tcgaaattct accgggtagg ggaggcgctt ttcccaaggc
    14221 agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct
    14281 ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc
    14341 ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg
    14401 cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca
    14461 gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct
    14521 ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg
    14581 ggctcagggg cggggcgggc gcccgaaggt cttccggagg cccggcattc tgcacgcttc
    14641 aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca
    14701 tccatctaga tctcgagcag ctgaagctta ccatgaccga gtacaagccc acggtgcgcc
    14761 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact
    14821 accccgccac gcgccacacc gtcgatccag atcgccacat cgagcgggtc accgagctgc
    14881 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
    14941 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
    15001 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
    15061 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
    15121 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
    15181 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
    15241 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
    15301 cctggtgcat gacccgcaag cccggtgcct gacgcccgcc ccacgacccg cagcgcccga
    15361 ccgaaaggag cgcacgaccc catgcatcga tgatatcaga tccccgggat gcagaaattg
    15421 atgatctatt aaacaataaa gatgtccact aaaatggaag tttttcctgt catactttgt
    15481 taagaagggt gagaacagag tacctacatt ttgaatggaa ggattggagc tacgggggtg
    15541 ggggtggggt gggattagat aaatgcntgc tctttactga aggctcttta ctattgcttt
    15601 atgataatgt ttcatagttg gatatcataa tttaaacaag caaaaccaaa ttaagggcca
    15661 gctcattcct cccactcatg atctatagat ctatagatct ctcgtgggat cattgttttt
    15721 ctcttgattc ccactttgtg gttctaagta ctgtggtttc caaatgtgtc agtttcatag
    15781 cctgaagaac gagatcagca gcctctgttc cacatacact tcattctcag tattgttttg
    15841 ccaagttcta attccatcag aagctggtcg agtaacttta aataattggc attatttaaa
    15901 gttaggcgcg ccataacttc gtatagcata cattatacga agttatgtcg agatatttaa
    15961 atattagacc cagctttctt gtacaaagtg gttgatatct ctatagtcgc agtaggcgga
    16021 taattacaag gaaggctggg gaagagggga cagtgaggtt aacaagaatg tcagtgactc
    16081 ccacagcatg accaagagga agctggtttg caccacacaa gggcaaaact tcagaaccaa
    16141 actttgtata gtcaatatct tcagaatcac aagtatggtc acttagtaga gcacacaagc
    16201 ctttattaca cgcattcatt gactttacgt atgcatggag gtgtgcctca agcacgtgga
    16261 agccagagac agcttacgga gggtggatcc ttctgacacg tggatcctgg gatcaaactc
    16321 gcgtcctcag gcttgggggc cagagcctgc acccgctgag ttatcttgac agaccagtga
    16381 agtaaaaatg tctggttact aacctttcct tcccactacc tctgctgggt ggtaggttag
    16441 aaagggaggt taaagccttt aaatgccctt attaaactag gttttaaaag atataagcca
    16501 gggctggaga gatggctcag cagttaagag cactgtctgc tcttctgaag gtcctgagtt
    16561 caaatcctaa caacaacatg gtggttcaca accatctgta atgagatctg atgccctctt
    16621 ctggggtatc tgaagacaac tatagtgtac ttacatataa taaataaata aatcttaaaa
    16681 aaaaatatat atatatatat atataagcct acagaccggc ttacaaagag aaaaataaag
    16741 aagccaggcg acctacttcc ctccaatcac tggttaagaa gccatcaaaa aaaaaaagta
    16801 aaaatgtcca cattaatttc ccagcctgcc aatatgttga cctttgccct gtctatacat
    16861 ctttctggca ctggttgttg aatttaatgc ccttgccaag tctaaagcaa aagtgtttcc
    16921 ccatgaactc tgactctact ctaaggacaa cctgattgac gctggatgaa atctacagga
    16981 catcccagca gtcgcatgtg gtagcatggt cctgtaattc cagctacttg ggaggctgaa
    17041 gcagatgggt ggtcagttcg aggcctgtct gggctgggct gggtacttgg caaggcctga
    17101 ctcaggttca gtgcttgcct agcacggatg ggaccctggg ctgaattcct ggcaccacca
    17161 gacctcccgg ggcctgtcta gaggagagtg gtagtgatcg ttgcttgagg ccaaacacta
    17221 cttgggatca ggaataggct gagggctgag ggtggtggta acatgcaccc gggaggcaga
    17281 agcaagatca caccaactgg aaggtagtct gggtaagact tccaggccat ttggggataa
    17341 atggtgaatc cctttctaca gatttcaaga gactaatgaa acagccctca acccaaccta
    17401 acctgggccc agcccacctc tgctgccacg gatttgagtt catgctgtct tcctgtctaa
    17461 ctgccaccca gcaatcactc cccaatccag tgctctgacc aatctcttgg agtagtgttt
    17521 ttcttggtgg tggggactga accacaggcc ttgggcatat gatcatggaa ccaaatctct
    17581 agtcccaaga tggagctttt actccagagt tggcaaaaat ctatcaaaat agcactcctg
    17641 gctagggaac tggtgtagtg gtacagcgct tgctaacacg cacaaggacc tggctttgac
    17701 ccctagcacc acaaacaaag cacccttggt aaggatccag ggcctcagta ggggtaaaga
    17761 agcaacgttt cccactgtat atccatcttc aagaactctg atgcctccaa tcctgccctg
    17821 cttttaaaaa attctcttct acttccccta cctcccccca aatctattta gcccttagcc
    17881 tccacctcca ctggtgctag gtgtccataa ccaaatggtc tgcttaagta ccaagctggc
    17941 catccacagg cctgggccct acaacacccc aaaagctccc cgcatgctta gctgacttct
    18001 tatgttcttt ccttgaccaa aatctctcca ctacttggtt gtgattacct catttttcgc
    18061 agtggtagtt gttgggcaaa ctatttccct ccatactgga gggtgtaaat gtccaagatc
    18121 caaagggagg ggcagagatt agaccctttc tacccacctg tcactagatt ccaaagagtc
    18181 aagcataaaa tgagtagtac tttaatcgtt tccaaagaag atggtacaag aatttagatt
    18241 agaaaatatg acattaaagg tgtattttcc tagaaatgaa aaacagaaca gaaacttaat
    18301 acaatttttt ttgatcttcc tcatacatca aaagctgtat ctgttcccag gctcgagctc
    18361 caagctccca ggctgctctt tatctccctc tagctgagca ggacaatccc tcctcctcca
    18421 cctctctcct ctcagtcacg cggtgtgggt gctaaaggcc ctaagctgac caatggacag
    18481 aagtgtcctt taagccagca gcccacaggc agtggcactc ggggcggtca cagaactggc
    18541 attgtttcca ctgtctcagc agaaagcagc ggaaagctga ccctcagcac aaatcagtca
    18601 aatgtggaac cggagcaagg gctcccaggc ccagggagga tgaccctttc ttactacaac
    18661 aggtctttaa gccgctctac tgcactccaa ggggattgaa agcatgtggc atctaaacaa
    18721 atcaggagaa cctgggaggc tgggggggag gggtggggat agtgataaac aggaatataa
    18781 atcagtttga tgatgtacag gggtgctggg aactccagct cttcacaggg cggccagccc
    18841 gccagaggac aacacagcaa gaaccacgac cgccactcca gacagccaga cctggggtat
    18901 tgccaggcct tttcctaggt cccatagctg tagaaaaaag aggggaagac aagtttttat
    18961 tttaattttc agcaattaga attccagtca ctcttacaat tcctgtctca gaaatgttct
    19021 acctactgaa cgggtacttc cttccctttt tggggttccg agaaaagcag catatcccta
    19081 tgtcccagtt tcacacttct gaaagcccca gtgatgaaaa tcaagtcatc tcatgtggta
    19141 ctgatgggtg actgctcttc agaatgccaa aggccctcca cacaaaagct tccagactgt
    19201 acagacataa tctcctctct ctagctgaca gcacatcgag atcagtgttt ctggaaaagt
    19261 cttccttcaa aacatagttt tgttttctga gatagaatct tgttattggg gctgggggtt
    19321 tgacttggaa gagagtatct gtctaggaag ccaacggccc tgggctcagc tctcggctgg
    19381 ggtggggaag atcttgttat atgattcaga ttagtgtaga attcatcctg tatcccagga
    19441 tggactcaaa tcaagctcct cctgccagca tttcccaagt gctggggtta caggtgttca
    19501 ccatatctgg accgaatgta cttctgcatc gtgtgggaca ttatgtggat gtggttaaaa
    19561 ttctgtggct ataactgcaa gattct
//